Selenoprotein evolution: introduction

Introduction to selenoprotein evolution

(more selenoprotein classes shortly)

SEPP2: lost in placentals

SEPP2 is a newly discovered paralog of SEPP1 with a UxxC motif (plus a later conserved cysteine and distal CxxC motif) and high-scoring putative SECIS element in 3' UTR that is quite conserved in vertebrates but only through marsupials. No corresponding gene -- or even decayed debris -- can be found in syntentic position in any placental mammal, including Atlantogenata. Also, cysteine has displaced the selenocysteine in Xenopus, a species much depleted in selenoproteins. SEPP2 does not appear at GenBank non-redundant other than mis-annotated SEPP1 chicken mRNAs. SEPP2 transcripts are available only in skate, zebrafish, and echidna (a short 454 read) .

The two paralogs have 4 coding exons with identical intron phases so clearly represent a segmental gene duplication. The gene duplication can be tracked back to before chondricthyes divergence but only one copy can be located in earlier diverging deuterostomes (sea urchin and acorn worm). SEPP2 retains the selenocysteine TGA only in its first exon and lacks the cluster of them in exon 4 of SEPP1. Exon 4 in SEPP2 is diverging very rapidlly, about neutrally, and is difficult to recover from blast searches without transcripts.

It can be surmised the first seleoncysteine in both SEPP1 and SEPP2 has a different functional role from terminal selenocysteines in SEPP1, possibly a conserved redox role distinct from selenostorage function of exon 4 in SEPP1. SEPP2 is very likely functional in all the species in which it occurs because of its conservation. There is no indication it is "on its way out" in marsupials -- the SECIS would be obliterated very quickly if the gene were non-functional.

SELU: 3 paralogs, variable timing losses

SELU: This family consists of three deeply diverged (distinct exon patterns) paralogs. The encoding gene has several average-length exons with anomalously short introns like many selenoproteins. In the SELU1 group, selenocysteine occurs in a UxxC motif already in the earliest deuterostomes but drops out in mammals after monotremes, being replaced by CxxC in marsupials and placentals. Amphibia separately lost selenocysteine.

The second paralog SELU2 has selenocysteine in bilaterans only to the node of sea urchin, suggesting it was lost early in the deuterostome ancestor (note though the early eukaryote Monosigna has cysteine). It is the closer paralog of SelU1, 36% vs 27% percent identity. No vestigal SECIS element persists in living species that encode cysteine. (The decayed SECIS elements still identifiable in 3' UTR of cysteine-containing GPX6 genes in rodents and human GPX5 represent much more recent loss of selenocysteine.)

The third paralog SELU3 has cysteine in all metazoans for which a sequence is available with the exception of the immediate outgroup choanflagellate Monosigna. It might be called virtual selenoprotein in eumetazoans. The pattern suggests a scenario in which selenocysteine was present in an ancestral gene prior to gene duplications followed by conversion to cysteine in different phylogenetic patterns within each gene subfamily.

This family exhibits the "selenocysteine rachet": if selenocysteine happens to be replaced by ordinary cysteine (despite putative catalytic inferiority) in some stem lineage, the unselected 3' UTR SECIS element then deteriorates over a few million years from accrued mutations, for the same reason (lack of purifying selection) the crayfish in the cave loses its imaging opsins. Consequently the whole following clade will contain cysteine -- a reversion to TGA at the cystein codon might occur but it would simultaneously require a multi-step reversion or de novo evolution of a SECIS element, ie all SECIS elements are ancient and selenocysteines cannot wink back on paraphyletically. (However the overall selenoproteome can still increase over time because of gene duplications elsewhere.)

A phylogenetic overview of the occurence of selenocysteine in SELU:

SELU1 SELU2 SELU3 genus species (common)
CFLC  CVVC  CYIC  Homo sapiens (human)
CFLC  CVVC  CYIC  Macaca mulatta (rhesus)
CFLC  CMVC  CVYC  Rattus norvegicus (rat)
CFLC  CMVC  CVYC  Mus musculus (mouse)
CFLC  ....  CVYC  Oryctolagus cuniculus (rabbit)
CFLC  CSVC  CYIC  Canis familiaris (dogs)
CFLC  CMVC  CYIC  Bos taurus (cow)
CFLC  ....  CYIC  Dasypus novemcinctus (armadillo)
CFLC  ....  ....  Echinops telfairi (tenrec)
CFLC  CMVC  CYIC  Monodelphis domesitca (opossum)
CFLC  ....  ....  Macropus eugenii (wallaby)
CFLC  CMVC  ....  Trichosurus vulpecula (opossum)
UFLC  ....  ....  Ornithorhynchus anatinus (platypus)
UFLC  ....  ....  Tachyglossus aculeatus (echidna)
UFLC  ....  CYTC  Gallus gallus (chicken)
UFLC  ....  ....  Taeniopygia guttata (finch)
UFLC  ....  ....  Anolis carolinensis (lizard)
CFLC  CQIC  CYTC  Xenopus tropicalis (frog)
CFLC  CQIC  CYTC  Xenopus laevis (frog)
UFLC  CQVC  CYTC  Danio rerio (zebrafish)
UFLC  CQIC  CYAC  Takifugu rubripes (fugu)
UFLC  CQVC  CSYC  Gasterosteus  aculeatus (stickleback)
UFLC  ....  CHTC  Oncorhynchus mykiss (trout)
UFLC  ....  ....  Ictalurus punctatus (catfish)
UFLC  CQVC  CSYC  Oryzias latipes (medaka)
UFLC  ....  ....  Callorhinchus milii (elephantfish)
UFLC  ....  ....  Squalus acanthias (spiny_dogfish)
....  CVVC  DYVA  Ciona intestinalis (tunicae)
UQLC  CQVC  ....  Branchiostoma floridae (amphioxus)
USLC  USAC  ....  Strongylocentrotus purpuratus (purple
....  ....  CYTA  Saccoglossus kowalevskii (acornworm)
....  UPFC  CFVC  Mytilus californianus (mussel)
....  UPFC  CFIC  Crassostrea gigas (bivalve)
....  UAFC  CFMC  Lottia gigantea (limpet)
....  UQLC  CFMG  Capitella capitatus (annelid)
....  UKLC  ....  Hydra magnipapillata (hydra)
....  UQLC  ....  Nematostella vectensis (anemone)
....  UQLC  ....  Oscarella carmela (sponge)
....  CRLC  UFCK  Monosiga brevicollis (choanoflagellate)

SEPW1: odd paralog SEPV gained in placentals

Selenoprotein SEPW1 is one of the shortest known mammalian proteins at 87 aa. With its CxxU motif, it is likely limited to simple redox reactions. Curiously, despite the small size protein still has 5 coding exons. One of these is of relatively recent origin because chondrichtyes and telost fish have the second and third exons fused (which, given the tree and extreme rarity of intron gain/loss must be the ancestral condition.

Like many genes on the densely packed human chromosome 19, SEPW1 seems to have given rise to a segmental duplication, here during the placental stem (ie, is absent marsupial and earlier diverging vertebrates). The second copy, called SELV, retains the same intron placements and phases but with greatly expanded and compositionally anomalous exon 1 which is highly prone to large indels (from replication slippage).

This long exon retains some conservation at its distal end to the last 5 residues of SEPW1 (unsurprising because the cysteine of the CxxU motif is at the end). There is observable conservation also at the amino terminus but otherwise the exon is evolving unconstrained. Possibly the initial methionine of SEPW1 was lost at the time of duplication but the terminal residues and splice donor were retained. Transcription far upstream lead to a remote methionine serving as replacement.

This is a very odd gene but transcripts in a half dozen species and moderate conservation (75% within placental mammals) in exons 2-5 over hundreds of millions of years witin placentals proves that it is evolving under selective constraint and so is functional. Distal exons however retain only 50% conservation to those of SEPW1, far too little to support ongoing gene conversion.

GPX3, GPX5, and GPX6: mixed retention of seleocysteine

The top image shows that the gene duplication leading to the divergently transcribed tandem pair GPX5 and GPX6 is quite old. Its stability to rearrangement suggests shared promoter and upstream regulatory elements. However the UCSC 28way alignment is misleading already at platypus because the 'tandem' shown really is just two instances of GPX3.

The pair is present in afrothere and xenarthra but missing in marsupial (which has only an otholog to GPX3). Hence the gene duplication event occured during the placental stem. No extant species exhibits the intermediate two gene state GPX3 + GPX4/5.

The lower image shows selenocysteine was lost to cysteine in GPX5 after platypus divergence but retained in earlier diverging lineages. GPX6 exhibits a bit of phylogenetic incoherence in its loss of selenocysteine in various placental subclades. GPX3 retains selenocysteine in all known vertebrate species.

These three paralogs have identical intron locations and phases, so represent a distinct subcluster of the overall set of seven GPX genes.

It is critical in annotating a complex gene family to build up bona fide sets of orthologs for each member validated by blast clustering, indels, intron pattern, and chromsomal synteny. These initial reliable sets then can correctly assign additional sequences to their correct class using them as classifying target database at Multalign.

DIO1, DIO2, and DIO3: a curious history

The iodothyronine deiodinases of selenoproteins have been thoroughly studied because of their role in thyroid hormone metabolism. The three paralogs seen in mammals diverged long ago -- the percent identity today is about 45% and exon breaks and phases are completely unrelated. One scenario is that DIO2 and DIO3 in mammals arose as an initial retroprocessed gene that then duplicated again, with DIO2 subsequently acquiring an intron and DIO3 remaining a single coding exon. The critical era for exon acquisition and loss is poorly represented in the databases.

DIO2 and DIO3 are found on the same chromosome arm of chr 14 in human but separated by 22 Mbp, rather far for a tandom duplication. This structure is conserved in the chicken genome with only 8.7 Mbp intervening. Again, it is hard to sort out coincidence from correlation. Note the SECIS element might well accompany both retroprocessing and tandem duplications.

No 3D structures are available for this family of proteins (which have a single N-terminal transmembrane segment) but deiodinases have thioredoxin-like fold (which includes glutathione peroxidase selenoproteins) according to SuperFamily. This domain might serve to define trimming for purposes of better protein trees as well as serve as a partial template for folding (noting too a short insert with strong similarities to the active site of iduronidase, a GH-A-fold of glycoside hydrolase).

DIO1 catalyzes the outer ring deiodination of T4 to the biologically active hormone T3, as well as the degradation of T3 and sulfated iodothyronines. DIO2 is also involved in outer ring deiodination. DIO3 converts T(4) to T(3) and T(3) to 3, 3'-diiodothyronine (T2) by inner-ring deiodination. This gene is imprinted and has an anti-sense transcript. These genes are of great interest in the evolution of the thyroid (resp. endostyle) and its hormones.

The putative redox motif varies significantly across the three paralogs, with the CxU motif of DIO1 and DIO3 becoming AxU in DIO2 (meaning it cannot form a local seleno-disulfide). A similar observation holds in some basal DIO3: calMil ATU not CTU, braFlo YTU not CTU. All three proteins contain a distal conserved cysteine; DIO2 has an additional conserved cysteine still further downstream.

DIO1 GSCTuPSF...PQCPV
DIO2 GSATuPPF...PQCRV...RVCIV...KRuKK
DIO3 GSCTuPPF...PGCAL

Remarkably, a second selenocysteine may have arisen in DIO2 near-terminal position post-lungfish divergence (ie after teleost fish divergence). This TGA codon is conserved over billions of branch length years and is presumably supported by the same SECIS element that supports the first selenocysteine. It may have arisen from read-through of a TGA stop codon; no cysteine counterpart is seen in this position in earlier diverging species DIO2 (fish, shark, lamprey) or in DIO1 or DIO3 paralogs. Since ancestral CTU in the proximal selenocysteine had already morphed into ATU by lamprey and chondrichthyes divergence, possibly the second selenocysteine now contributes the second half of a redox pair (as alanine has a chemically inert sidechain). Bioinformatics alone cannot be definitive here -- the possibility remains that this TGA is the actual stop codon and became conserved for some other reason, or that multiple losses of selenocysteine occured in the earlier diverging species.

>DIO2_homSap  PFSYNLQEVRHWLEKNFSKRuKKTRLAG*
>DIO2_panTro  PFSYNLQEVRHWLEKNFSKRuKKTRLAG*
>DIO2_ponPyg  PFSYNLHEVRHWLEKNFSKRuKKTRLAG*
>DIO2_macMul  PFSYNLQEVRHWLEKNFSKRuKKTRLAG*
>DIO2_calJac  PFSYNLQEVRHWLEKNVSKRuKKTRLAG*
>DIO2_otoGar  PFFYNLQEVRRWLEKNFSKRuNRLAG* 
>DIO2_micMur  PFFYNLQEVRHWLEKNFSKRuNLD*
>DIO2_tupBel  PFYYNLQEVRRWLEKNFSKRuNSLAG*
>DIO2_musMus  PFSYNLQEVRSWLEKNFSKRuILD*
>DIO2_ratNor  PFSYNLQEVRSWLEKNFSKRuILD*
>DIO2_speTri  PFCYNLQEVRHWLEKNFSKRuILD*
>DIO2_cavPor  PFCYNLQEVRRWLEKNFHKRuNRLDG*
>DIO2_oryCun  PFSYNLQEVRHWLEKNFSKRuKKTRLAG*
>DIO2_ochPri  PFCYNLQEVRRWLEKNFNKRuI*
>DIO2_canFam  PFYYNLQEVRRWLEKNFSKRuNRLAG*
>DIO2_equCab  PFCYNLQEVRHWLEKSFSQRuNRLC*
>DIO2_myoLuc  PFFYNLQEVRHWLEKNFNKRuNLD*
>DIO2_pteVam  PFFYNLQEVRRWLEKNFNKRuNLD*
>DIO2_bosTau  PFFYNLQEVRRWLEKNFSKRuKRLAG*
>DIO2_turTru  PFYYNLQEVRRWLEKNFSKRuKLD*
>DIO2_susScr  PFYYNLQEVRRWLEKNFSKRuKLD*
>DIO2_vicVic  PFHYNLQEVRRWLEKNFSKRuNLD*
>DIO2_eriEur  PFCYNLQEVRHWLEKSFSQRuNLD*
>DIO2_sorAra  PFCYNLQEVRDWLEKNFSKRuNSL*
>DIO2_dasNov  PFCYNLQEVQHWLEKNFSKRuNRLAG*
>DIO2_choHof  PFYYSLQEVRQWLEKNFSKRuNLD*
>DIO2_echTel  PFCYNLQEVRCWLEKNFSKRuTRVAGQ*
>DIO2_monDom  PFFYNLQEVRLWLEKNFSKRuNPG* 
>DIO2_macEug  PFFYNLQEVRLWLE-NFSKRuNPG*
>DIO2_ornAna  PFFYNLQEVRLWLEQNFSKRuNPD*
>DIO2_galGal  PFFYNLQEVRLWLEQNFSKRuNPLSTEDLSTDVSL*
>DIO2_taeGut  PFFYNLQEVRLWLEQNFSKRuNPFSTGVTSIDVSL*
>DIO2_anoCar  PFFYNLQEVRLWLEQNFRKRuNPNLPQEDMLNISPLK**
>DIO2_phiOlf  PFFYNLQEVRLWLEQNFRKRuNPNLPDKKSRGLSF*
>DIO2_xenTro  PFFYNIQEIRRWLELSFGKRuT*
>DIO2_neoFor  PFFYNLKEVRHWLEQTYRKRuVPTCELIM*
>DIO2_danRer  PFFYNLKDVRRWLEKCYGK**
>DIO2_tetNig  PFFYNLKEVRQWLEHSYGKR*
>DIO2_takRub  PFFYNLKEVRQWLEQSYGKR*
>DIO2_gasAcu  PFFYNLKDVQHWLEQNFGKRFSRTSAEKDMSHISKKGIIHQ*
>DIO2_oryLap  PFFYNLKEVRQWLEQLRQTVGPNTEE**
>DIO2_funHet  PFFYSLKDVRQWLELSYGRR*
>DIO2_oncMyk  PFFYNLKDVRQYLEQSYGKR*
>DIO2_parOli  PFFYNLKDVQKWLEQSYGKR*
>DIO2_calMil  PFFYNLNGVREWLERHSGQRWG*
>DIO2_petMar  PFFYRVREVKSFLESVKASR*

The collection of DIO1 below from 49 species illustrates the importance of rare genomic events in defining the topology of the mammalian species tree. Here we see a 5 residue insert in the first exon restricted to Pegasoferea (ie excluding artiodactyls) with no hint of homoplasy. Additionally a longer deletion is restricted to Afrotheres.

DIO1_homSa   MGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQ
DIO1_panTr   ..................................................................................................H.............
DIO1_macMu   .....S...V.K................................................................................................D...
DIO1_calJa   ....G..................A......T.......K......D........N...........................................H.........D...
DIO1_otoGa   ....R..........F.......A...M..........SQ.....QQ.V.AK.............LQHPV.LVCPEGPL.....M..R.........SAS...K....D...
DIO1_musMu   .....LW......VIF.Q..LE.A.....MT...G...QS.....Q....A...R.AP...V.....I................RA.F.......T..C....K....D.I.
DIO1_ratNo   ...S.LW......VIF.Q..LE.AT....MT...E...Q......Q........R.AP...V.....I................RA.Y.......T.......K..V.D.I.
DIO1_oryCu   ....R...........VQ...E.A.....MT...E...Q......Q...IAQ..N.AQ.S........................A..P.......S.......Q.S..D..R
DIO1_cavPo   ...TW...........VQ...E.AM....MT...E.I.KS..............Q..................I.........E.A.......D.S..C...EKRT..D..H
DIO1_canFa   ....R.V...R......Q...Q.A....F.K...A...QH.V..NGN-----K....Y...A..LY.M.........Q......R..P....................D...
DIO1_ursAr   ....R.V...R......Q..M..A..........E...QQV...NK.-----.....Y...L...Y.M................R..P....................D... 
DIO1_felCa   ...S.L....R.....FQ..LQ.A....F.....S...QH.V..NR.-----.....Y...A..LY.V................R..P.................S..D..K
DIO1_equCa   ....RA...........Q..LQ.A......T.......QH.V..NQ.-----.....Y...V..LY...........H........KR......S.............D...
DIO1_myoLu   ..............I..Q..L..TL...Q.K...R...QH....NR.-----.....Y...A..L...P....I..........K..E.S...............H..D...
DIO1_pteVa   .E..W..R.........Q..L..A....Q.T...R...Q..V..NR.-----.....F...L..L...............Q.....KE..........C.........D...
DIO1_sunMu   ....GL..L...FG..VR..LK.A......T.W.SAIRPHL...S.....AK..R.TYED.A...............N..Q...R.KQ.DI..DS...H.....ARL.D...
DIO1_bosTa   ....S...........FQ..L..AI.....T...R...Q...................E..............I..........M..Q..............E..S..D...
DIO1_turTr   ....L...........FQ.GL..AM.....T...R...Q.....S.....AK.....YE........A.....I..........M..Q..R.................D...
DIO1_susSc   .E..L...........FQ..L..AM....MT...G...QD....SQ....AK......E........A................K..E..........S......H..D...
DIO1_vicVi   ...SL...........FQ.VL..AL.....T...G...QD....SQR...AQ.....YE..............I.............Q.....D....C..-D.VH..D...
DIO1_eriEu   ....S...........FQ..L..AI.....T...R...Q...................E..............I..........M..Q..............E..S..D...
DIO1_dasNo   ...S..........I.FQ..L..A...T..T...G...Q....KSQ.SHKAE....PY...G....N......L..IG......K..Q..........H.........D...
DIO1_choHo   ...SW............Q..L..AM..I..T...G...Q.....SRRANN.KD.Q.PY...G....N.................K..Q..........H..R......D...
DIO1_loxAf   ..............IF.K..L..AM.........G...K....Q--------....AY.M.GS.L..IP....I...Y......K..E..P..D....C........SD...
DIO1_proCa   ......V..........R..L..AM.....A...G...K....Q--------....AY.M.CS.L..VP........Y......K..E..........H.....R...D...
DIO1_monDo   .LRLWLW.........Q.VG..LM..LMKM.S...M.QH..G..Q.SSIFQ..N.KYE..G....TLP..L...R........QALQ..P..D....S.R..PRRL.D..HA
DIO1_triVu   . AG.L..VR.F.A..Q..F......L.KT...NMM.KH..SL.QRSSISQ.TQ.AYE..G.....I...F............QALQ......P...T.K.ESRH..D..H
DIO1_anoCa   . FKA.RLVLKT.L..Q.CLSTA...LFM....ATA..Y..KQS.RSS.G...N.VYE..G.....F..LL.....K.K....KALQ.CP...T...DFD.KIHH.LD...

SELH: rapid evolution

SELH is another small selenoprotein with a conserved CxxU redox motif split by a phase 21 intron. The introns in mammalian SELH are exceedingly short (eg, 93 and 162 bp in human with gene coding span very short at 619 bp) but the level of transcription is not remarkable so provides no explanation for the lack of retroposons. Some species like orangutan have processed pseudogenes, implying transcription in germ-line tissues. Zebrafish has a diverged duplicate gene with tryptophan at the seleocysteine site in addition to an intronless transcribed gene with TGA selenocysteine -- it appears that this has displaced the normal three-intron gene (seen in other fish). That can work in selenoproteins since retropositioning begins at the 3' end of transcripts, meaning the SECIS likely accompanies the coding region.

Protein conservation is below average -- SELH percent identities (to human) drop to the low 80's within Laurasiatheres, to 72% with marsupial, and 57% with chicken. Further, rodents exhibit significant residue loss upstream of the CxxU motif, very unusual in such a short protein but indicative of an inessential structural region. Indeed observed conservation is primarily centered in the middle of the protein. No phylogenetic conversion of selenocysteine to cysteine is observed within vertbrates

SELM: retained in ER

Another selenoprotein of unknown function, SELM with CxxU motif, surfaces during Blastp searches using selenoprotein SEP15 (which oddly has a CxU motif) as query. A 3' UTR SECIS element cannot be located with SECISearch 2.19 yet it seemingly must be located in the comparative genomic peaks of conservation lying between SELM and the neighboring gene.

Since the protein is quite short with 5 introns, complete SELM sequences are best recovered from cDNAs and genomic alignments in the UCSC 28way. This is an ancient protein recoverable from vertebrates, amphioxus, sea urchin, shrimp, mite, moths, and hydra, and plants. Moths (silkworm and hawkmoth) -- but not their outgroups -- encode cysteine in a CxxC motif in place of CxxU. No loss of selenocysteine is seen in 22 species of phylogenetically dispersed vertebrates.

SELM may begin with a signal peptide. There are no glycosylation sites nor additional conserved cysteines beyond the CxxU motif. SuperFamily finds no similarity to proteins of known 3D structure. The terminal residues appear to be a phylogenetically conserved KDAL-class endoplasmic retention signal

In whole-mount zebrafish embryos, SELM is expressed within the notochord and anterior somites, axial fin fold, dorsal spinal chord neurons, then in lateral line neuromasts. SelM is located in the ER/Golgi as is its distant homolog Sep15 (found associated with UDP-glucose glycoprotein glucosyltransferase, an ER-resident protein involved in quality control of protein folding).

MSRB123: methionine sulfoxidases

MSRB1 is a short odd protein, rich in cysteines, with two CxxC motifs, a near-amino terminal cysteine and a more distal selenocysteine in a motif with serine. MSRB is now known to be a stereospecific methionine-R-sulfoxide reductase repairing oxidative damage to methionine in native proteins. The two pairs of cysteines bind a zinc atom. The all-beta structure has been determined in a bacterial homolog with a internal structural duplication so weak it needs an xray determination to be revealed. Humans have 9 such domains in 9 proteins according to SuperFamily. These could potentially encode other selenoproteins at least in some species.

The fold exists as a small family of paralogs, three in mammals, with specialization to cell compartment -- cytosol, mitochondria and endoplasmic reticulum (via KAEL* signal), respectively.). All contain catalytic zinc. This multiplicity of MSRB contrasts with a single non-homologous methionine-S-sulfoxide reductase (not a selenoprotein in any species).

MSRB1 has selenocysteine in its active site in conserved motif UxxS, whereas MSRB2 and MSRB3 contain Cys in the well-conserved motif CINS. The cysteine in the Ux53xC motif of prokaryotes is replaced by serine or threonine in all eukaryotic MSRBs as U/CxxS/T. While serine and threonine have polar hydroxyl groups reminiscent of a cysteine, whether they form a covalent bond with the selenocysteine or otherwise contribute to catalysis remains unresolved. Thus this protein has a defunct CxxU motif that was unusual among selenoproteins in having 53 intervening residues.

The exon structures, relative to reliably locatable CxxC anchors, show MSRB2 and MSRB3 more closely related (having a distinctive break within the second CxxC motif), whereas MSRB1 introns are placed altogether differently. This suggests two rounds of gene duplication with intronation of MSRB2/3 prior to the second round. A tree based on the alignable conserved core of these proteins indicates the same result.

The phylogenetic distribution of orthologs of MSRB1 is orderly, with selenocysteine back through fish, with no counterpart currently locatable in amphioxus or tunicates (which otherwise have 3 MSRB genes). Further, there is no sporadic appearance of selenocysein in MSRB2/3 -- cysteine is always found, even prior to bilatera, suggesting though selenocysteine is ancestral (viz prokaryotes) but became cysteine prior to the second duplication giving rise to MSRB2/3.

Care must be taken at greater depth because of possible confusion among paralog members (and lineage-specific expansions and contractions). Synteny is lost so intron phasing and siting must be used along with blast clustering. The selenoprotein rachet predicts as more species are sequenced, some clades may exhibit lost selenocysteine in MSRB1 but none will acquire it in MSRB2 or MSRB3 lineages.

This raises the question that if selenocysteine is not necessary, why is it retained in MSRB1? Possibly that has some connection to localization in the reduced cytosol whereas more oxidizing mitochondrial and ER compartments utilize cysteines. In this view, selenocysteine will not be lost in any vertebrate clade no matter how many species are sequenced for MSRB1; whereas in MSRB23 cysteine was an adaptive change rather than mere rift, allowing wider intracellular distribution. Evidently methionine sulfoxide forms in all three compartments.

32 vertebrate MSRB1 aligned in exon 3:
VSCGKCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFVPK	homSap Homo sapiens (human)
VSCGKCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFVPK	panTro Pan troglodytes (chimp)
VSCGKCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFVPK	ponPyg Pongo pygmaeus (orang_sumatran)
VSCGKCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFVPK	macMul Macaca mulatta (rhesus)
VSCGRCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFIPK	otoGar Otolemur garnettii (bushbaby)
VSCGRCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	micMur Microcebus murinus (mouse_lemur)
VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFVPK	musMus Mus musculus (mouse)
VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	ratNor Rattus norvegicus (rat)
VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	cavPor Cavia porcellus (guinea_pig)
VSCGKCGHGLGHEFLNDGPKPGQSRFuIFSSSLKFIPK	oryCun Oryctolagus cuniculus (rabbit)
VFCGKCGHRFGHEFLNDGLKPGQSRFuIFSNTLKFVPK	ochPri Ochotona princeps (pika)
VSCGKCGNGLGHEFLNDGPKPGKSRFuIFSSSLKFIPK	canFam Canis familiaris (dog)
VSCGRCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFIPK	felCat Felis catus (cat)
VSCGRCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	bosTau Bos taurus (cow)
VSCGRCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	susScr Sus scrofa (pig)
VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFVPK	equCab Equus caballus (horse)
VSCGRCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPR	eriEur Erinaceus europaeus (hedgehog)
VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	loxAfr Loxodonta africana (elephant)
VSCGKYGHGLGHEFLNDGPNWGQSRFuIFSSSLKFIPK	echTel Echinops telfairi (tenrec)
VSCGKCGNGLGHEFLNDGPKKGQSRFuIFSNTLKFVPK	triVul Trichosurus vulpecula
VSCGKCGNGLGHEFLNDGPRRGQSRFuIFSSSLKFIPK	ornAna Ornithorhynchus anatinus (platypus)
VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK	galGal Gallus gallus (chicken)
VLCGKCGNGLGHEFINDGPKKGQSRFuIFSSSLKFVPK	anoCar Anolis carolinensis (lizard)
VSCGKCGNGLGHEFINDGPKKGQSRFuIFSSSLKFIPK	xenTro Xenopus tropicalis (frog)
VRCGKCGNGLGHEFVNDGPKHGLSRFuIFSSSLKFIPK	danRer Danio rerio (zebrafish)
VRCGKCGNGLGHEFLNDGPSRGLSRFuIFSSSLKFIPK	tetNig Tetraodon nigroviridis (pufferfish)
VRCGKCGNGLGHEFVNDGPSRGLSRFuIFSSSLRFIPK	takRub Fugu rubripes (fugu)
VRCGKCGNGLGHEFVNDGPAKGVSRFuIFSSSLKFIPK	gasAcu Gasterosteus  aculeatus (stickleback)
VRCGKCGNGLGHEFVNDGPSKGLSRFuIFSSSLKFIPK	oryLap Oryzias latipes (medaka)
IRCGKCNNGLGHEFLNDGPKHGLSRFuIFSSSLKFV..	ictFur Ictalurus furcatus (fish)
VRCGKCGNGLGHEFVGDGPKKGLSRFuIFSSSLKFV..	oncMyk Oncorhynchus mykiss (trout)
VRCGKCGNGLGHEFVGDGPKKGLSRFuIFSSSLKFV..	salSal Salmo salar (salmon)
.SCGKCGNGLGHEFLNDGLKAGQSRYuIFSNSLKFVPK	calMil Callorhinchus milii (elephantfish)

SEPHS1 and SEPHS2: selenophosphate synthetases

Selenophosphate synthetases are biosynthetic enzymes that capture selenium in a chemical form suitable for selenoprotein biosynthesis. One of the two mammalian selenophosphate synthetase paralogs, SEPHS2, itself contains a selenocysteine in a conserved UGCK motif several dozen residues in from the amino terminus. This motif contains threonine as TGCK in SEPHS1 which apparently functions in a selenium salvage system recycling catabolic selenocysteine (tRNA is charged with precursor serine), whereas the SEPHS2 enzyme functions in selenite assimilation (selenophosphate from selenide and ATP). Unsurprisingly only SEPHS2 has a selenocysteine insertion sequence (SECIS) element in 3' UTR.

This gene family has a curious evolutionary history: every additional level of phylogenetic resolution adds another twist -- even 29 genomes from cnidarian to placental do not provide adequate genomic saturation. The original single-copy gene (itself an ancient internal tandem duplicatation fold with active site on dimeric cleft) procedes uneventfully from ur-bacteria to hagfish/lamprey divergence.

However, prior to teleost fish divergence, block duplication of the 8 exon gene took place. No flanking synteny persists in extant species. The copy that would remain closest to the ancestral gene then lost its selenocysteine to nucleophile threonie, its 3' UTR SECIS insertion element decayed, and the old function strayed to salvaging selenide from free selenocysteine (arising from selenoprotein catabolism or diet). The copy we call SEPHS2 diverged rapidly ( rather unconstrained amino terminus in both length and sequence) but with conservation about the selenocysteine active site motif and beyond.

Between monothere and marsupial divergence, a fully processed retrogene (no introns) carrying the 3' UTR SECIS insertion element became functionally established, remarkably displacing the previous 8-exon gene (now an undetectable decayed pseudogene or deletion), to the extent that every species of placental mammal retains only the processed retrogene and threonine copies. Opossum, but not platypus nor elephant or tenrec, retains all 3 gene copies.

Here the processed retrogene mechanism (begin at 3' end of UTR but not extend necesarily to initial methionine or 5' regulatory regions) brings along the SECIS selenocysteine insertion loop making a new selenoprotein feasible and while explaining non-homologous start peptides. Humans have an assortment of fragmentary and processed pseudogenes for which SEPHS1 is usually the parent, most notably a mis-spliced processed pseudo omitting exon 7 (build 35, chr7:63756925-63757879).

SELO: rapid change in last exon

The terminal exon containing the selenocysteine is evolving very erratically in length, suggesting that is unimportant.

VRRVLKLLETPYHCEAGAATDAEATEADGADGRQR                   SYSSKPPLWAAELCVTuSS*  SELO_homSap Homo sapiens (human)
VRRVLKLLETPYHCESGAATDAEATEANGADGRQR                   SYSSKPPLWAAELCVTuSS*  SELO_panTro Pan troglodytes (chimp)
VRRVLKLLETPYHCEAGAATDAEATEADGADGRQR                   SYSSKPPLWAAELCVTuSS*  SELO_ponPyg Pongo pygmaeus (orang_sumatran)
VRRVLKLLETPYHCEAGAATDAEATEADGADGRQR                   SYSSKPPLWAAELCVTuSS*  SELO_macMul Macaca mulatta (rhesus)
VQRVLKLLETPYDNGGGAAAEPKDGSRAASRRP                     SYSSKPPLWAAELCVTuSS*  SELO_otoGar Otolemur garnettii (bushbaby)
VRRVLKLLESPYHSEEEATGPEAVARSTEEQS                      SYSNRPPLWAAELCVTuSS*  SELO_musMus Mus musculus (mouse)
VRRVLKLLESPYHSEEEATGPEAVARTTDEQS                      SYSSRPPLWAAELCVTuSS*  SELO_ratNor Rattus norvegicus (rat)
VRRVLKLLESPYQHEGEHAEALEVAGPEGAATGASRRP                SYSSKPPLWAAELCVTuSS*  SELO_cavPor Cavia porcellus (guinea_pig)
VRRVLELLETPYHRAEEAARVPEATEPEGASGADSGGH                SYSSKPPLWAAELCVTuSS*  SELO_canFam Canis familiaris (dog)
VRRVLKLLETPYGGEAEAEAAEPAEASEAAEETGGAAGRRR             SYSSKPPLWAAELCVTuSS*  SELO_bosTau Bos taurus (cow)
VRRVLKLLETPYHREGEAAEPAEPEAAEGRL                       SYSSKPPLWAAELCVTuSS*  SELO_susScr Sus scrofa (pig)
VRRVLKLLEAPYHRAEEAAEVSEAAEPEGAGGASGRRR                SYSSKPPLWAAELCVTuSS*  SELO_equCab Equus caballus (horse)
VRRVLKLLEAPYHREGEAAEVLEAAEPEGAGSTAERRR                SYSSKPPLWAAELCVTuSS*  SELO_myoLuc Myotis lucifugus (microbat))
VRRVLKLLETPYPQEREAPEALEAAEPQGAGRQD                    SYSSRPPLWAAELCVTuSS*  SELO_eriEur Erinaceus europaeus (hedgehog)
VRRVLKLLEAPYSREEPTEALELGEAAGAVGLQR                    SYSSRPPPWAAELCVTuSS*  SELO_loxAfr Loxodonta africana (elephant)
VQRVLRLLEKPYGEPWEDDADGLLAAAAAADSGEAESRR               SYGRKPPLWAAELCVTuSS*  SELO_monDom Monodelphis domestica (opossum)
VRKVAKLLEHPYREEEGPDDGEGTADRSVRGL                      AYGGKPPHWAAQLCVTuSS*  SELO_ornAna Ornithorhynchus anatinus (platypus)
VRNVLKLLENPFQETEDSTEMETKEEEATATAAACAQATRSRL           SYCSKPPLWASELCVTuSS*  SELO_galGal Gallus gallus (chicken)
VKRVLQMLENPYQEGESCQSIADKSPEEDVAVAASSVSTNPSRL          PYNSKPPLWATELCVTuSS*  SELO_xenTro Xenopus tropicalis (frog)
VQRVLKVLEKPFSVQEGLEQPGWMGRGGAAIPGERDETEEEGSNSSGAGARGLVPYDSKPPVWANEICVTuSS*  SELO_danRer Danio rerio (zebrafish)
VQEERLRVMEGTNPEFPAWVGGSGEAANQGERDEGEEQQQAMASSSTPRNP  VSYDSKPPAWAGEICVTuSS*  SELO_takRub Takifugu rubripes (fugu)
VHLLQKTLRHPFHKQREAEEA                                 GYSSRPPLWARELRVSCSS*  SELO_calMil Callorhinchus milii (elephantfish)

Selenoprotein sequences available from genome project species

...... DIO1 DIO2 DIO3 GPX1 GPX2 GPX3 GPX4 GPX5 GPX6 GPX7 MSRB1 MSRB2 MSRB3 SELH SELI SELK SELM1 SELM2 SELO SELS SELT SELU1 SELU2 SELU3 SELV SEPHS1 SEPHS2 SEPN SEPP1 SEPP2 SEPW TXNRD1 TXNRD2 TXNRD3
homSap DIO1 DIO2 DIO3 GPX1 GPX2 GPX3 GPX4 GPX5 GPX6 GPX7 MSRB1 MSRB2 MSRB3 SELH SELI SELK SELM1 SELM2 SELO SELS SELT SELU1 SELU2 SELU3 SELV SEPHS1 SEPHS2 .... SEPP1 ..... SEPW TXNRD1 TXNRD2 TXNRD3
panTro DIO1 .... .... GPX1 GPX2 .... .... .... .... .... ..... ..... ..... .... .... SELK ..... ..... .... SELS .... ..... ..... ..... SELV ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
ponPyg DIO1 .... .... .... .... .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... SEPW ...... ...... ......
macMul DIO1 DIO2 DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 ..... ..... SELH .... SELK ..... ..... .... SELS SELT ..... ..... ..... SELV ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
calJac DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... ..... ..... SEPW ...... ...... ......
tarSyr .... .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
otoGar DIO1 DIO2 .... .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... SELK ..... ..... .... SELS SELT ..... ..... ..... SELV ...... ...... .... ..... ..... .... ...... ...... ......
micMur DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... SEPW ...... ...... ......
tupBel DIO1 DIO2 .... GPX1 GPX2 .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
musMus DIO1 .... DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 MSRB2 MSRB3 SELH .... SELK ..... SELM2 SELO SELS SELT ..... ..... ..... SELV SEPHS1 SEPHS2 .... SEPP1 ..... SEPW ...... ...... ......
ratNor DIO1 DIO2 DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 ..... ..... SELH .... SELK ..... SELM2 SELO SELS SELT ..... ..... ..... SELV ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
cavPor DIO1 .... DIO3 .... GPX2 .... .... .... .... .... MSRB1 ..... ..... .... .... SELK ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... ..... ..... SEPW ...... ...... ......
speTri DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
dipOrd DIO1 .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
oryCun DIO1 .... .... GPX1 GPX2 .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... ..... ..... SEPW ...... ...... ......
ochPri DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... SEPW ...... ...... ......
canFam DIO1 DIO2 DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 MSRB2 MSRB3 SELH .... SELK ..... SELM2 .... SELS SELT ..... ..... ..... SELV ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
felCat DIO1 .... DIO3 GPX1 GPX2 .... .... .... .... .... ..... ..... ..... .... .... SELK ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... ..... ..... SEPW ...... ...... ......
equCab DIO1 DIO2 .... GPX1 GPX2 .... .... .... .... .... MSRB1 ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... SEPP1 ..... .... ...... ...... ......
myoLuc DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... SELK ..... ..... .... SELS .... ..... ..... ..... SELV ...... ...... .... ..... ..... .... ...... ...... ......
pteVam DIO1 .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... ..... ..... .... ...... ...... ......
bosTau DIO1 DIO2 DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 MSRB2 ..... SELH .... SELK ..... SELM2 .... SELS SELT ..... ..... ..... SELV ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
oviAri .... .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
susScr DIO1 DIO2 DIO3 .... .... .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... SELM2 .... SELS .... ..... ..... ..... SELV ...... ...... .... ..... ..... SEPW ...... ...... ......
turTru DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
vicVic DIO1 .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
eriEur DIO1 .... .... GPX1 GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... SELS .... ..... ..... ..... .... ...... ...... .... ..... ..... SEPW ...... ...... ......
sorAra .... DIO2 .... GPX1 GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
dasNov .... .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
choHof DIO1 .... .... .... GPX2 .... .... .... .... .... ..... ..... ..... SELH .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... SEPP1 ..... SEPW ...... ...... ......
loxAfr DIO1 .... .... .... GPX2 .... .... .... .... .... MSRB1 ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... SELV SEPHS1 SEPHS2 .... ..... ..... .... ...... ...... ......
proCap DIO1 .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... SELV ...... ...... .... ..... ..... .... ...... ...... ......
echTel DIO1 DIO2 DIO3 .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
monDom DIO1 DIO2 DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 ..... ..... SELH .... .... ..... ..... .... SELS SELT SELU1 ..... ..... .... SEPHS1 SEPHS2 .... ..... SEPP2 SEPW ...... ...... ......
macEug .... .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... SELU1 ..... ..... .... ...... SEPHS2 .... ..... SEPP2 .... ...... ...... ......
ornAna DIO1 DIO2 DIO3 GPX1 GPX2 .... GPX4 .... .... GPX7 MSRB1 MSRB2 MSRB3 SELH SELI SELK SELM1 SELM2 SELO SELS SELT SELU1 ..... ..... .... SEPHS1 SEPHS2 .... SEPP1 SEPP2 SEPW ...... ...... ......
tacAcu .... .... .... GPX1 .... .... GPX4 GPX5 .... GPX7 ..... ..... ..... SELH .... SELK SELM1 ..... SELO SELS .... SELU1 ..... ..... .... SEPHS1 SEPHS2 .... SEPP1 ..... .... ...... ...... ......
galGal .... DIO2 DIO3 GPX1 GPX2 GPX3 .... .... .... .... MSRB1 ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... ..... .... ...... ...... ......
taeGut .... .... .... .... .... .... .... .... .... .... ..... ..... ..... .... .... SELK ..... ..... .... .... .... SELU1 ..... ..... .... ...... ...... .... ..... SEPP2 .... ...... ...... ......
anoCar DIO1 DIO2 .... GPX1 .... .... .... .... .... .... MSRB1 ..... ..... .... .... SELK ..... ..... .... .... .... SELU1 ..... ..... .... ...... ...... .... ..... SEPP2 SEPW ...... ...... ......
xenTro DIO1 DIO2 DIO3 GPX1 GPX2 .... .... .... .... .... MSRB1 MSRB2 ..... .... .... SELK ..... SELM2 .... SELS .... ..... ..... ..... .... SEPHS1 SEPHS2 .... SEPP1 SEPP2 SEPW ...... ...... ......
danRer DIO1 DIO2 DIO3 GPX1 .... .... .... .... .... .... MSRB1 ..... ..... SELH .... .... ..... SELM2 .... SELS .... ..... ..... ..... .... SEPHS1 ...... .... SEPP1 SEPP2 SEPW ...... ...... ......
tetNig .... DIO2 DIO3 GPX1 .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... SEPP2 .... ...... ...... ......
takRub DIO1 DIO2 DIO3 GPX1 .... .... .... .... .... .... ..... ..... ..... SELH .... .... ..... ..... .... .... .... SELU1 ..... ..... .... ...... ...... .... ..... ..... SEPW ...... ...... ......
gasAcu .... DIO2 DIO3 GPX1 .... .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... SEPP2 .... ...... ...... ......
oryLap .... DIO2 DIO3 GPX1 .... .... .... .... .... .... ..... ..... ..... SELH .... SELK ..... ..... .... .... .... ..... ..... ..... .... ...... ...... .... ..... SEPP2 .... ...... ...... ......
calMil DIO1 DIO2 DIO3 .... GPX2 .... .... .... .... .... ..... ..... ..... .... .... .... ..... ..... SELO SELS .... SELU1 ..... ..... .... SEPHS1 ...... .... ..... SEPP2 SEPW ...... ...... ......
petMar .... DIO2 .... .... GPX2 .... .... .... .... .... ..... ..... MSRB3 .... .... .... SELM1 ..... SELO SELS SELT ..... ..... SELU3 .... SEPHS1 ...... SEPN ..... ..... SEPW ...... ...... ......
braFlo .... .... DIO3 .... .... .... .... .... .... .... ..... ..... ..... .... .... SELK ..... ..... .... .... .... SELU1 ..... ..... .... SEPHS1 ...... .... ..... ..... .... ...... ...... ......

Reference set of vertebrate selenoproteins

SELH: 25 vertebrate sequences

>SELH_homSap Homo sapiens (human) NP_734467 Selenoprotein H exons chr11 80% identity musMus
0 MAPRGRKRKAEAAVVAVAEKREKLANGGEGMEEATVVIEHC 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNPTKPRRGSFEVTLLRPDGS 1
2 SAELWTGIKKGPPRKLKFPEPQEVVEELKKYLS* 0

>SELH_ponPyg Pongo pygmaeus (orang_sumatran)
0 MAPRGRKCKAEATVVAVAEKrEKLTNGGEGMEEATIVIEHC 2
1 TSuRVYGRNAAALSQVLCLEAPELPVKVNPTKPRRGSFEVTLLRPDGS 1
2 SVELWTGIKKGPPCKLKFPEPQEVVEKLKKYLS* 0

>SELH_macMul Macaca mulatta (rhesus) 
0 MAPRGRKRKAEAAMVAAAEKQEKLANSGEGMEETTVVIEHC 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNPSKPRRGSFEVTLLRPDGS 1
2 SAELWTGIKKGPPRKLKFPEPQEVVEELKKYLS* 0

>SELH_micMur Microcebus murinus (mouse_lemur)
0 MAPRGRKRKAEASVVATAEKREKLENGGEAVEEATVVIEHC 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNPAKPRRGSFEVTLQRPDGS 1
2 SAELWTGIKKGPPRKLKFPEPQVVVKELKKYL.* 0

>SELH_tupBel Tupaia belangeri (tree_shrew)
0 MAPRGRKRKAEAAVVATAEKQEKLQNGGEGVKEASIVIEHC 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNSAKPRRGSFEVTLLRPDGS 1
2 SVELWTGIKKGPPRKLKFPEPQEVVEELKKYLS* 0

>SELH_musMus Mus musculus (mouse)
0 MAPHGRKRKAGAAPMETVDKREKLAEGATVVIEHC 2
1 TSuRVYGRHAAALSQALQLEAPELPVQVNPSKPRRGSFEVTLLRSDNS 1
2 RVELWTGIKKGPPRKLKFPEPQEVVEELKKYLS* 0

>SELH_ratNor Rattus norvegicus (rat) TGA verified 77%
0 MAPLGRKRKAGAAPIESADKREKLAEGAAVVIEHC 2
1 TSuRVYRRHAAALSQALQLEAPEISVQVNRSKPRRGSFEVTLLRPDNS 1
2 RVELWTGIKKGPPRKLKFSEPQEMVEELKKYLS* 0

>SELH_speTri Spermophilus tridecemlineatus (squirrel)
0 MAPRVRKRKAEAAAVSTSEKREKLENGKEQVEEAVVIEHc 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNPSKPRRGSFEVTLLRRDGTS 1

>SELH_oryCun Oryctolagus cuniculus (rabbit)
0 MAPGKRKRKAEAAPVASAEKREKLANGGQGVEEIVIEHc 2
1 tSuRVYGRNAAALSQALRLQAPELPVTVNPSKPRRGSFEVTLLRPDGS 1
2 gAELWTGIKKGPPRKLKFPEPQQVVEELKKYLS* 0

>SELH_ochPri Ochotona princeps (pika)
0 MAPNRRKRKAEAVADAAAEKREKQAKQANGVGGGEEIVIEHc 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNPAKPRRGSFEVTLQRPDGS 1
2 SAELWTGIKKGPPRKLKFPEPQQVVEELKKYLS* 0

>SELH_canFam Canis familiaris (dog)
0 MASRGRKRKAEAAGVAAAEKRDKPASGRKAVEEATVVIEHC 2
1 TSuRVYGRNAAALSQALRLETPELPVEVNPAKPRRGSFEVTLLRPDGS 1
2 SVELWTGIKKGPPRKLKFPEPQEVVKALKQHLS* 0

>SELH_bosTau Bos taurus (cow) TGA verified
0 MASRGRKRKAEAALAAAAEKREKPAGGQEGGVEGPSVVIEHC 2
1 TSuRVYGRNAAALSQALRLQAPELTVKVNPARPRRGSFEVTLLRADGS 1
2 SAELWTGLKKGPPRKLKFPEPHVVLEELKKYLS* 0

>SELH_oviAri Ovis aries (sheep)
0 MASRGRKRKAEAALAAAAEKREKPAGSREGEVAGPSVVIEHC 2
1 TSuRVYGRNAAALSQALRLQAPELAVKVNPSRPRRGSFEVTLLRADGS 1
2 AELWTGLKKGPPRKLKFPEPHVVLEELKKYLS* 0

>SELH_susScr Sus scrofa (pig)
0 MASRGRKRKAETALGAAAEKQETPASGRKGMEEPSVVIEHC 2
1 TSuRVYGRNAAALSQALRVEAPELPVRVNPTKPRRGSFEVTLMRPDGS 1
2 SAELWTGIKKGPPRKLKFPEPQEVVEELKKYLS* 0

>SELH_equCab Equus caballus (horse)
0 MASRGRKRKAEAVVAVAEKREKLTSGGKGVEEVTVVIEHc 2
1 TSuRVYGRNAAALSQALRLEAPELPVKVNPAKPRRGSFEVTLLRPDGS 1
2 sAELWTGIKKGPPRKLKFPEPQEVVEELKKYLS* 0

>SELH_choHof Choloepus hoffmanni (sloth)
0 MASRGRKRKAEAVVLAAAEKQEKVASGKEGEKEAVVVIEHC 2
1 TSuRVYGRNAAALSQALRLEAPEIPVKVNFAKPRRGSFEVTLLRPDGS 1
2 TELWTGIKKGPPRKLKFLQPLKVVEELKKYLS* 0

>SELH_loxAfr Loxodonta africana (elephant)
0 MASRGRKRKAEAAVAAAAAEKREKPVGGQAAAEEVVVIIEHC 2
1 TSuRVYGRNAAALSQALRLEAPELSVKVNPSKPRRGSFEVTLQRPDGS 1
2 GAELWTGIKKGPPRKLKFPEPQEVVEELKKYL* 0

>SELH_monDom Monodelphis domestica (opossum)
0 MAPRGRKRKADVAAAALTEKPEKLAQGGEEGAGEARVVIEHC 2
1 QSuRVYARHAEAVGQALRLARPGLPVLLNPAKPRRSSFEVTLLRPDGS 1
2 RVELWSGIKKGPPRKLKFPEPAQVVEELKARLV* 0

>SELH_ornAna Ornithorhynchus anatinus (platypus) E5DI3CH10F50JR
0 DAGGAEVGEGLHVVIEHC 2
1 RSuGVYGRRAEALSRALSLAAPDLPVLLNPTKPRRNSFEVTLLRPDGT 1
2 RTELWSGIKKGRP

>SELH_galGal Gallus gallus (chicken) tga confirmed
0 MAPRGRKRAARRPAEPEARADPPEKRPRDEAEGSPGDAGGPRVVIEHC 2
1 RSuRVYGRNAAALSEALRGAVASLAVEINPRQPRRNSFEVSLVKEDGS 1
2 TVQLWSGIGKGPPRKLKFPEPAAVVEALRSSLA* 0

>SELH_danRer Danio rerio (zebrafish) AI877878 BM037625 CKSU tga verified exons fused
0 MATRGKSARKRKADSDEKEKLDDAKKEKLEDKDEETGLRVVIEHC 2
1 KSuRVYGRNADVVREALADSHPELKVMINPHKPRRNSFEITLMDGERADVLWSGIKKGPPRKLKFPEPAEVVTALKQALEKE* 0

>SELH_takRub Takifugu rubripes (fugu) tga confirmed, no Ests 3 exons
0 MTPRVLMTGRRGTKRKAEEDEKPKEEKKEKQREDDQGGPRVAIEHC 2
1 KSuRVYGRNAEAVKSALLAAHPGLTVVLNPEKPRRNSFEITLLDEGK 1
2 ETSLWTGIKKGPPRKMKFPQPDVVVTALQEALKTE* 0

>SELH_ictPun Ictalurus punctatus (catfish) CB940790 tga verified 
0 MATRAKAGRGAKRKADVIAAAEPVAKQDKGNKGEREDDEGQRVIIEHC 2
1 KSuRVYGRNADAVREALLSAHPELHVVLNPEKPRRNSFEVTLIEGK 1
2 KELVLWTGLKKGPPRKLKFPEPAEVVTALEEALKSK* 0

>SELH_salSal Salmo salar (salmon) CA043802 tga verified 
0 MASRNKAGRVLKRKASVKEESVEEKRGKGEDDQPEIVTEGRRVVIEHC 2
1 KSuRVYGRNAEGVRVALLAACPDLTVVLNPQKPRSKSFEVILVEGE 1
2 KEVCLWSGIKKGPPRKLKFPEPEVVVSALEKALKTE* 0

>SELH_oryLat Oryzias latipes (medaka) BJ026077 tga verified 
0 MASKAGRRGTKRKVEAKKEEDKTSTEEKKARGENAHEEAGLKVLIEHC 2
1 KSuRVYGRNAEEVKSALLAARPELTVVCNPEKPRRNSFEITLLDGAK 1
2 ETSLWTGIKKGPPRKLKFPQPDDVVAAFKDALKTE* 0

>SELH_oncMyk Oncorhynchus mykiss (trout) BX312781
0 MASLTKAGRVLKRKVETEESSVEGKRGKGEDDHPEIVTEGQRVVIEHC 2
1 KSuRVYGRNAEGVRVALLAACPDLTVVLNPQKPRSKSFEVILFEGE 1
2 KEVCLWSGIKKGPPRKLKFPEPEVVVSALEKALKTE* 0

SELI: 2 mammalian sequences

>SELI_homSap Homo sapiens (human) chr2
0 MAGYEYVSPEQLAGFDKYK 0
0 YSAVDTNPLSLYVMHPFWNTIVK 0
0 VFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYAS 1
2 APGHKHVPDWVWIVVGILNFVAYTL 1
2 DGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQV 0
0 TISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMII 1
2 GCALCVTLPMSLLNFFR 2
1 SYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANST 0
0 CQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTLTTAFTLAHIHYGVRV 0
0 VKQLSSHFQIYPFSLRKPNSDuLGMEEKNIGL* 0

>SELI_ornAna Ornithorhynchus anatinus (platypus) 53% LOC100086265
0 MAGYEYVSAEQLAGFDKYK 0
0 YSALDTNPLSLYVMHPFWNTIVKV 0
0 IFPTWLAPNLITFSGFLLLVFNFLLMAYFDPDFYAS 1
2 APGQKHVPDWVWIVVGILNFTAYTL 1
2 DGVDGKQARRTNSSTPLGELFDHGLDSWACVYFVVTVYSIFGRGRTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDMSQV 0
0 TISIVYIVTAVVGVEAWYEPFLFNFLYRDLFTTMII 1
2 GCAVTVTLPMSLYNF 2
1 AYRNKTLKYSSVYETMLPFVSPCLLFTLSTTWIFLSPSNILETHPRLFYFMVGTLFANIT 0
0 CQLIVCQMSNTRCQPLNWLLMPLALVILVVHSGLAPHSETFLLYSLTALVTVAHIHYGIRV 0
0 VNQLSKHFKILPFSLRKPSSDuLGLEEEKIGL* 0

SELK: 32 vertebrate sequences

>SELK_homSap Homo sapiens (human) chr3 last coding bp and stop codon split off into 5th exon
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRSYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_panTro Pan troglodytes (chimp)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRSYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_ponPyg Pongo pygmaeus (orang_sumatran)
0 MVYISN 1
2 AQVLDSWSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDLKKRRSYGNSSDFRYDDDGRG 2
1 PPGNPPGRMGPINHLGGPSPPPMAGGuGR 2
1* 0

>SELK_macMul Macaca mulatta (rhesus)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRSYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_otoGar Otolemur garnettii (bushbaby)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRGYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLQGPSPPPMAGGuGR 2
1* 0

>SELK_micMur Microcebus murinus (mouse_lemur)
0 MSYISN 1
2 GQLLDSRSQSPWRLSLITDFFWRKPEFVVLF 2
1 FKTLLQQDVKKRRGCGNSSDSRYDDGRG 2
1 PPGNPFQRMGQINHLHGPSPPMAGGuGR 2
1* 0

>SELK_tupBel Tupaia belangeri (tree_shrew)
0 MVYISN 1
2 GRVSDSWSQSPWRLSLITDFFWGMAKFVVLS 2
1 FKTLLQQDVRKRKGYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRISHLRGPSPPPVAGGuGR 2
1* 0

>SELK_musMus Mus musculus (mouse)
0 MVYISN 1
2 GQVLDSRNQSPWRVSFLTDFFWGIAEFVVFF 2
1 FKTLLQQDVKKRRGYGSSSDSRYDDGRG 2
1 PPGNPPRRMGRISHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_ratNor Rattus norvegicus (rat)
0 MVYISN 1
2 GQVLDSRNQSPWRLSFITDFFWGIAEFVVFF 2
1 FKTLLQQDVKKRRGYGGSSDSRYDDGRG 2
1 PPGNPPRRMGRISHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_speTri Spermophilus tridecemlineatus (squirrel)
0 MVYISK 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVILF 2
1 FKTLLQQDVKKRRGYGNSSDSRYDDGRG 2
1 PPGNPPRRMGGINHLQGPSPPPMGGGuGR 2
1* 0

>SELK_cavPor Cavia porcellus (guinea_pig)
0 MVYISN 1
2 GQVLDNRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKGRGYRNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLQGPSPPPMAGGuGR 2
1 * 0

>SELK_oryCun Oryctolagus cuniculus (rabbit)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLL 2
1 FKTLLQQDVKRGRGYRNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_ochPri Ochotona princeps (pika)	
0 MVYISN 1
2 GQVLDSRSQSPERLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKRRRDYRSSSDSRYDDGRG 2
1 LPGNPPRRMGRIYHLRCPSPPPPPGGGuGR 2
1* 0

>SELK_canFam Canis familiaris (dog)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRGYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_neoVis Neovison vison (mink)	
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRGYGSSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1* 0

>SELK_felCat Felis catus (cat)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVVLL 2
1 FQTLLQQDVKKRRGYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_equCab Equus caballus (horse)
0 MVYISN 1
2 GQVLDSRSQSPWRLSFITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRRGYGSSSDSRYDDGRG 2
1 PPGNPRRRMGRINHLHGPSPPPMAGGuGR 2
1 * 0

>SELK_myoLuc Myotis lucifugus (microbat)
0 MVYIEN 1
2 RQVLDSRSQSPwRLSLITDFFWGIAEFVVLF 2
1 FKTLLQKDMKKGRGYGNSSDSRYDDGRG 2
1 PPGNPPRRMGRIDHLRGPSPPPMAGGuGR 2
1* 0

>SELK_bosTau Bos taurus (cow)
0 MVYISN 1
2 GQVLDSRSQSPWRLSFITDFFWGIAEFVVLF 2
1 FRTLLQQDVKKRRGYGSSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLQGPNPPPMAGGuGR 2
1 * 0

>SELK_susScr Sus scrofa (pig)
0 MVYISN 1
2 GQVLDSRSQSPWRLSFITDFFWGIAEFVVLF 2
1 FRTLLQQDVKKRRGYGGSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPNPPPMAGGuGR 2
1 * 0

>SELK_dasNov Dasypus novemcinctus (armadillo)
0 MVYISN 1
2 GQVLDSRSQSPWRLSLITDFFWGIAEFVILL 2
1 FRTLLQQDVKKKRGYGSSSDSRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_loxAfr Loxodonta africana (elephant)
0 MVYISN 1
2 GQVLDSRSQSPWRLSFITDFFWGIAEFVVLF 2
1 FKTLLQQDVKKRGGYGSSSDFRYDDGRG 2
1 PPGNPPRRMGRINHLRGPSPPPMAGGuGR 2
1 * 0

>SELK_ornAna Ornithorhynchus anatinus (platypus) EUEMSW407EBWK3 454
0 MVYISN 1
2 GHVLNGQNRSLWSLSFIKDFFWGILDFIIMF 2
1 FKSMIHPNVKRGCRNSSSDSKYDDGRG 2
1 PPGYPRRGMGRINHSNGPSPPPMAGGGuGR 2
1 * 0

>SELK_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01DCGU7 454
0 MVYISN 1
2 GHVLNGQNRSPWSLSYIKDFFWGILDFIIMF 2
1 FKSMIHPNVKRGCRNSSSDSKYDDGRG 2
2 PPGYPRRGMGRINHSNGPNPPPMAGGuGR 2
1 * 0

>SELK_anoCar Anolis carolinensis (lizard)
0 MVYISN 1
2 GQVLDNQSRAPWSFSSITDFFWGIADFVVMF 2
1 FQSIIHPDLTRGSTSSSSSRYDDGRG 2
1 PPGLPRRRMGRISHFGGPSPPPMAGGuGR 2
1 * 0

>SELK_galGal Gallus gallus (chicken)
0 MVYISN 1
2 GQVLDNRSRAPWSLSAITDFFWSIADFVVMF 2
1 FQSIIQPDLRRRGYTSSSYLGQSDGRG 2
1 PPGNPRRRMGRINHWGGGPSPPPMAGGGuGR 2
1 * 0

>SELK_taeGut Taeniopygia guttata (finch)
0 MVYISN 1
2 GQVLDSQSRAPWRLSSITDFFWSIADFVVLF 2
1 FQSIIQPDLRRRGYTSSSYSGYHDRRG 2
1 PPANPRRRMGRINHWGGGPSPPPMAGGGuGR 2
1* 0

>SELK_xenTro Xenopus tropicalis (frog)
0 MVYISN 1
2 GQVLDGQSRSPWRLSFLTDMFWGITDFVVMF 2
1 FQSIIHPNVTRRGCQNSSSSTRYDDGRG 2
1 PPGHPRRMGRINHGSGPSAPPMAGGGGuGR 2
1 * 0

>SELK_oncMyk Oncorhynchus mykiss (trout)
0 MVYVAN 1
2 GQVLDNRSQSPWRMSFLTDLFWGAVEFIGLF 2
1 FQSLVQPDLTKRGNSGSSSTGYSDGRG 2
1 PPGPPGGRRRMGRINHGGGPSAPPMGGGGuGR 2
1 * 0

>SELK_salSal Salmo salar (salmon)
0 MVYVAN 1
2 GQVLDNRSQSPWRMSFLTDLFWGAVEFIGLF 2
1 FQSLVQPDLTKRGNSGSSSAGYSDGRG 2
1 PPGPPGGRRRMGRINHGGGPSAPPMGGGGuGR 2
1 * 0

>SELK_oryLap Oryzias latipes (medaka) BJ003636  tag confirmed
0 MVYVSN 1
2 GQVLDSRAQSPWRLSLLVDLFWDALEFFRLF 2
1 FKTMFHPDLTKDGNSASSRFSDGRG 2
1 PPGPPGGRRRIGRINHGAGPNAPPMGGGGuGR 2
1 * 0

>SELK_braFlo Branchiostoma floridae (amphioxus) tga confirmed
0 MVYVTG 1
2 GQMLENRSPWRLSIIPEIFWGFINFIVLF 2
1 FQTMYNPSLSKHGSDTPSSYSRGDGRG 2
1 PPPPPPRRRFGGVRRGGAPGPPPMAGGGuGR 2
1 * 0

SELO: 5 vertebrate sequences

>SELO_homSap Homo sapiens (human) tga-sel taa-stop
0 MAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRWLAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLR
QPRLVALSEPALALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSR 2
1 QADGRKVLRSSIREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIR 2
1 FGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFRE 0
0 VTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDR 2
1 YDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPELPLELGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLT 1
2 GADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPR 2
1 QLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYR 2
1 ARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSE 0
0 VRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVTuSS* 0

>SELO_musMus Mus musculus (mouse) 
0 MAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRWLAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLR
QPRLVALSEPALALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSR 2
1 QADGRKVLRSSIREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIR 2
1 FGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFRE 0
0 VTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDR 2
1 YDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPELPLELGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLT 1
2 GADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPR 2
1 QLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYR 2
1 ARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSE 0
0 VRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVTuSS* 0

>SELO_ratNor Rattus norvegicus (rat)
0 MASFRAAFGASLAVARTRPQCVGLELQSSAPWSAWAAAMEPTPRWLARLRFDNRALRALPVETPPPGPEDSLSTPRPVPGACFSRARPAPLR
QPRLVALSEPALALLGLEVSEEAEVEAEAALFFSGNALLPGTEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTAAGERWELQLKGAGPTAFSR 2
1 QADGRKVLRSSIREFLCSEAMFHLGIPTTRAGACVTSESTVMRDVFYDGNPKYEKCTVVLRIAPTFIR 2
1 FGSFEIFKPPDELTGRAGPSVGRNDIRVQMLDYVISSFYPEIQAAHTCDTDNIQRNAAFFRE 0
0 VTRRTARMVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFLDR 2
1 YDPDHVCNASDNAGRYTYSKQPQVCRWNLQKLAEALEPELPLVLAEAILKEEFDTEFQRHYLQKMRKKLGLVRVEKEDETLVAKLLETMHQT 1
2 GADFTNTFCVLSSFPAEPSDTAEFLTQLTSQCASLEELKLAFRPQMDPR 2
1 QLSMMLMLAQSNPQLFALIGTQANVTKELERVEHQSRLEQLSPSELQSKNRDHWETWLQEYR 2
1 ERLDKEKEGVGDIAAWQAERVRIMHANNPKYVLRNYIAQKAIEAAENGDFSE 0
0 VRRVLKLLESPYHSEEEATGPEAVARTTDEQSSYSSRPPLWAAELCVTuSS* 0

>SELO_ornAna Ornithorhynchus anatinus (platypus) uc003bjx.1
0 2
1 HADGRKVLRSSIREFLCSEAMFHLGIPTTRAGTCVTSESAVIRDVHYDGNPKYEKCAVVLRIASTFLR 2
1 FGSFEIFKPRDEHTGRQGPSVGRNDIRIRMLDYVIGTFYPEIEEANADDTVRRNAAFFRE 0

>SELO_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01DJRM3 454
0 YGGHQFGTWAGQLGDGRAHLIGIYTNRHGEQWELQLKGSGRTPYSR 2
1 NGDGRAVFRSSVREFLGSEAMHYLRIPTSR

>SELO_galGal Gallus gallus (chicken)
0 MQRSGGVLRRGRADTERGETGGGWLSALRFDNLAMRSLPVDPFEDCAPRAVPGACFARVRPTPLRNPRLVAMSAPALALLGLEAGGPEAERE
AEAALYFSGNRLLPGSEPAAHCYCGHQFGSFAGQLGDGAAIYLGEVRGPRGARWELQLKGAGITPFSR 2
1 QADGRKVLRSSIREFLCSEAMFHLGIPTTRAGTCVTSDSEVVRDIFYDGNPKKERCTVVLRIASTFIR 2
1 FGSFEIFKPPDEYTGRKGPSVNRNDIRIQMLDYVIGTFYPEIQEAHADNSIQRNAAFFKE 0
0 ITKRTARLVAEWQCVGFCHGVLNTDNMSIVGLTIDYGPFGFMDR 2
1 YDPEHICNGSDNTGRYAYNRQPEICKWNLGKLAEALVPELPLEISELILEEEYDAEFEKHYLQKMRKKLGLIQLELEEDSKLVSELLETMHLT 1
2 GGDFTNIFYLLSSFSVDTDPSRLEDFLEKLISQCASVEELRVAFKPQMDPR 2
1 QLSMMLMLAQSNPQLFALIGTKANINKELERIEQFSKLQQLTAADLLSRNKRHWTEWLEKYR 2
1 VRLHKEVESISDVDAWNTERVKVMNSNNPRYILRNYIAQNAIEAAENGDFSE 0
0 VRNVLKLLENPFQETEDSTEMETKEEEATATAAACAQATRSRLSYCSKPPLWASELCVTuSS* 0

>SELO_calMil Callorhinchus milii (elephantfish) frag tgt-cys not sel tag-stop? tga-stop? taa-stop
0                 LNFDNLALRSLPVDSSGERSCRRVPGACFSLAGATPVDNPRLVASSR    0
0 QSDGRKVLRSSIREFLCSEAMFHLGIPTTRAGTCVTSDTKVLRDVFYDGNSKHENCTIILRIAPTFLRF 2
1 FGSFEILKPEDELTGRQGPSSNRNDIRIQMLDYVIGTFYPEAQQAHPENQVQRNAAFFRE 0
0 VTQRTARLVAEWQCVGFCHGVLNTDNMSIMGLTIDYGPFGFMDR 2 
1 FDPSYICNASDNRGRYAYNQQPEICKWNLGKLAEVLVPELPLKDSQSIIDEEYDTEFQRHYLQKMRKKLGLLQCEQEDDDKLVSELLDIMYRT 1
2 GADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPR 2
1 QLSILLMLSQSNPQLFEVIGSKEGIAKELDLIERSSKLQQATAEDIHSNNAKVWTEWLQKYR 2
1 SRLATEAEGVDDVDEQNAERVKVMNLNNPKFILRNYIAQNAIEAAEKGDFSE 0
0 VHLLQKTLRHPFHKQREAEEAGYSSRPPLWARELRVSCSS-R-P* 0

>SELO_petMar Petromyzon marinus (lamprey) frag
0        GASMCSLYVLPLGRN 1
2 APRQVPGAVFSRVRPSPVERPRVVAISVPALRLLGLRDPEAEAARPEAAEFLSGNRVPPGAQPAAHCYCGHQFGSFAGQLGDGAAMYLGEVQPGPGQRWEVQLKGAGPTPYS 2
1 HSDGRKVLRSSLREFLCSEAMHHLGVPTTRAGSCVTSHSTVLRDVHYDGNARPEQCSVVLRIAPSFLR 0
1 FGSFEIFKSTDKDTGRTGPSAGREDIKVTMLDYVIDTFYPELLEGHGDGASHKYTAFFRE 0
0 VVRRTAHLVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFMDR 2
1 GADFTNSFRLLSRLTLEPGSVEELATLLCQQCATVEEMKRAYKPRIDPR 2

SEPN: 2 vertebrate sequences

>SEPN_homSap Homo sapiens (human)
0 MGRARPGQRGPPSPGPAAQPPAPPRRRARSLALLGALLAAAAAAAVRVCARHAEAQAAARQ 0
0 ELALKTLGTDGLFLFSSLDTDGDMYISPEEFKPIAEKLT 1
2 GSTPAASCEEEELPPDPSEETLTIEARFQPLLPETMTKSKDGFLG 0
0 VSRLALSGLRNWTAAASPSAVFATRHFQPFLPPPGQELGEPWWIIPSELSMFTGYLSNNRFYPPPPKGKE 0
0 VIIHRLLSMFHPRPFVKTRFAPQGAVACLTAISDFYYTVMFR 2
1 IHAEFQLSEPPDFPFWFSPAQFTGHIILSKDATHVRDFRLFVPNHR 2
1 SLNVDMEWLYGASESSNMEVDIGYIPQ 0
0 MELEATGPSVPSVILDEDGSMIDSHLPSGEPLQFVFEEIKWQQELSWEEAARRLEVAMYPFKK 0
0 VSYLPFTEAFDRAKAENKLVHSILLWGALDDQSCu 1
2 GSGRTLRETVLESSPILTLLNESFISTWSLVKELEELQ 0
0 NNQENSSHQKLAGLHLEKYSFPVEMMICLPNGTV 0
0 VHHINANYFLDITSVKPEEIESNLFSFSSTFEDPSTATYMQFLKEGLRRGLPLLQP* 0

>SEPN_petMar Petromyzon marinus (lamprey) tga-sel taa-stop frag
0 0
0 EMALRTLGNDGLFLFTSLDTNMDMQISPEEFRPIVDKII 1
2 GPPPSEYEGTQEADPQGEGLTMLARFEPLLMETMSKSRDGFLG 0
0 VGQSCLAGLRGWKKAEAPSQHFGANQFKVFLPPKSDLELGEAWWLVPNDLNLFTGYLPNSRYYPPPPVAKE 0
0 IIIFKLLSMFHPRPFVKSRFAPQGSVACIRAQSDMYYDIVFR 2
1 VHAEFQLNEPPAFPFWFTPAQFTGHVTIARDSSHVRAFHMFVPNNR 2
1 SLNVDMEWLFGSMDQGNMEVDIGFMPK 0
0 MELVAEGPSVPALIYDENGNAINTSDPDVEPIQFVFENIEWRSEISFQEAYRQMEVAMYPFKK 0
0 IQYHPFTEAFEKAKAEDKLVHSILLWGALDDQSCu 1
2 KVLTQSLKFDFNITSPVLALLSENFISSWSLVKDLEDL 0
0 KQEEQAEHAKWATLHLAKYTFPVEMMIALPNGTV 0
0 VHCINANDFLDATAVKAEDLTPDLPAEFLDPTSTTYLKFLKEGLQKAQTYLQA* 0

SELS: 17 vertebrate sequences

>SELS_homSap Homo sapiens (human)
0 MERQEESLSARPALETEGLRFLHTT 1
2 VGSLLATYGWYIVFSCILLYVVFQKLSARLRALRQRQLDRAAAAV 1
2 EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQ 0
0 LEEEKRRQKIEMWDSMQEGKSYKGNAKKPQ 0
0 EEDSPGPSTSSVLKRKSDRKPLRGG 1
2 GYNPLSGEGGGACSWRPGRRGPSSGGuG* 0

>SELS_panTro Pan troglodytes (chimp)
0 MERQEESLSARPALETEGLRFLHTT 1
2 VGSLLATYGWYVVFSCILLYVVFQKLSARLRALRQRQLDRAAAAV 1
2 EPDVVVKRQEAVAAARLKMQEELNAQVEKHKEKLKQ 1
0 LEEEKRRQKIEMWDSMQEGKSYKGNAKKPQ 0
0 EEDSPGPSTSSVLKRKSDRKPLRGG 1
2 GYNPLSGEGGGACSWRPGRRGPSSGGuG* 0

>SELS_macMul Macaca mulatta (rhesus)
0 MEREEDSLSARPALETEGLRFLHVT 1
2 VGSLLASYGWYIVFSCILLYVVFQKLSARLRALRQRQLERAAASV 1
2 EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQ 0
0 LEEEKRRQKIEMWDSMQEGKSYKGNAKKPQ 0
0 EEDSPGPSTSSVVKRKSDRKPLRGG 1
2 GYNPLSGEGGGSCSWRPGRRGPSSGGuS* 0

>SELS_otoGar Otolemur garnettii (bushbaby)
0 MEEEAEPLPARPALEAEGLRFLHVT 1
2 VGSLLAAYGWYIVFSCVLLYVVFQKFSTRLRALRQRQLDRAAAAV 1
2 EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQ 0
0 LEEEKRRQKIEMWDSMQEGKSYKGNTKKPQ 0
0 EEDNSGPSVIPKRKSDRKPLRGG 1
2 GYNPLSGEGGGACSWRPGRRGPSSGGuG* 0

>SELS_musMus Mus musculus (mouse)
0 MDRDEEPLSARPALETESLRFLHVT 1
2 VGSLLASYGWYILFSCILLYIVIQRLSLRLRALRQRQLDQAETVL 1
2 EPDVVVKRQEALAAARLRMQEDLNAQVEKHKEKLRQ 0
0 LEEEKRRQKIEMWDSMQEGRSYKRNSGRPQ 0
0 EEDGPGPSTSSVIKGKSDKKPLRGG 1
2 GYNPLTGEGGGTCSWRPGRRGPSSGGuG* 0

>SELS_ratNor Rattus norvegicus (rat)
0 MDRGEEPLSARPALETESLRFLHVT 1
2 VGSLLASYGWYILFSCVLLYIVIQKLSLRLRALRQRQLDQAEAVL 1
2 EPDVVVKRQEALAAARLRMQEDLNAQVEKHKEKLRQ 0
0 LEEEKRRQKIEMWDSMQEGRSYKRNSGRPQ 0
0 EEDGPGPSTSSVIPKGKSDKKPLRGG 1
2 GYNPLTGEGGGTCSWRPGRRGPSSGGuS* 0 

>SELS_canFam Canis familiaris (dog) tga confirmed   
0 MERDGQQLSARPALETEGLRYLHVT 1
2 VGSLLATYGWYIVFSCILLYVVFQKLSTRLRALRQRQLDRAEAAV 1
2 EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLRQ 0
0 LEEQKRRQKIEMWDSMQEGKSYKGNARKHP 0
0 EEDSPGPSTSSVLPKRKPDRKPLRGG 1
2 GYNPLSGEGGGACSWRPGRRGPSSGGuG* 0

>SELS_equCab Equus caballus (horse)
0 MEPDEERLSARPAVEAEGLRFLHVT 0
2 GSLLAAYGWYLVFSCILLYVLFQKLSSRLRALRQRRLDRAAAAV 1
2 EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLRQ 0
0 LEEEKRRRKIEMWDSMQEGKSYKRNARGPQ 0
0 EEDSPGPSTSSVIKRKSDRKPLRGG 1
2 GYNPLSGEGGGACSWRPGRRGPSSGGuG* 0

>SELS_bosTau Bos taurus (cow) tga confirmed     
0 MERDGDQLSARPTLETEGLRFLHVT 1
2 VGSLLATYGWYIVFSCILLYVVFQKLSTRLRALRQRHLDQAAAAL 1
2 EPDIVVKRQEALAAARLKMQEELNAQVEKHKEKLRQ 0
0 LEEEKRRQKIEMWDSMQEGKSYKGNTRKPQ 0
0 EEDSPGPSTSSVIPKRKSDRKPLRG 1
2 GGYNPLSGEGGGTCSWRPGRRGPSSGGuG* 0

>SELS_susScr Sus scrofa (pig)
0 MEQDGDQLSARPALETESLRFLHVT 1
2 VGSLLATYGWYIVFCCILLYVVFQKLSTRLRALRQRHLDGAAAAL 1
2 EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLRQ 0
0 LEEEKRRQKIERWDSVQEGRSYRGDARKRQ 0
0 EEDSPGPSTSSVIPKRKSDKKPLRGG 1
2 GYNPLSGEGGGACSWRPGRRGPSSGGuG* 0 

>SELS_eriEur Erinaceus europaeus (hedgehog)
0 MEADTEPLPLRPALETEGLRLLHAT 1
2 VGSLLASYGWYIVLCGVVLYVAFQKLSPRLRALRRRQLDRAEAAV 1
2 EPDVVVKRQEALAAARLKMQEELNAQAEKHKEKLKQ 0
0 LEEERRRQKIEMWDSMQEGKSYRGNSRRAQ 0
0 EDDSPGPSTSSAVKRKPDRKPLRGG 1
2 GYNPLSGEGGGSCSWRPGRRGPSSGGuG* 0

>SELS_dasNov Dasypus novemcinctus (armadillo)
0  1
2 LGSLLATYGWYIVFSCVLLYVVFQKLSTRLRALRQRQLDRAAAAV 1
2 EPDIVVKRQEAVAAARLKMQEELNAQAEKHKEKLKQ 0
0 LEEEKRRQKIEMWDSIQEGKYGNAKKP 0
0 KGDCPGSSTSLVMKCQSAKLRGG 1
2 GYNPLSGEGGGTCSWRPGRRGPSAGGuG* 0

>SELS_monDom Monodelphis domestica (opossum)
0 MDLDEEEQQQLLAKPALELEGIRFLQET 1
2 VGWLLSNYGWYLLFSFIILYVIFQKLFFRVMRQRALESVASSI 1
0 EPDTVVKQQEALAASRLRMQEELNTQAEKYKEKLKQ 0
2 LEEEKRKQKIEMWESMQEGKSYKGKAKKPQ 0
0 QEPEPGPSTSSNIPKPKPDRKSLRGS 1
2 GYNPLTGEGSGTCSWRPGRRGPSSGGuG* 0
 
>SELS_ornAna Ornithorhynchus anatinus (platypus)
0 LFI 1
2 VGSVLSAYGWYILFGCAVLYLIFQKLSGSLRVMRRRYSDTTGAAI 1
2 DPEVVVKRQEALAASRLRMQEELNAQAEKYREKQKQ 0
0 LEEAKRRQKIEIWESMQEGKSYKGNSRLQPQ 0
0 QETDPGPSTSSVIPKPKPARKPLRG 1
2 GYNPLSGEGGGTCSWRPGRRGPSSGGuG* 0

>SELS_tacAcu Tachyglossus aculeatus (echidna) EUEMSW404CHB0V 454
0 1
2                               LRVMRQRYSDTTGAAI 1
2 DPEVVVKRQEALAASRLRMQEELNAQAEKYREKQKQ 0
0 LEEAKRRQKIEIWESMQEGKSYKGNSRLRPK 0
0 QETDPGPSTSSVIPKPKPARKPLRGG 1
2 SYNPLSGEGGGTCSWRPGRRGPSSGGuG* 0

>SELS_galGal Gallus gallus (chicken)
0 MELGDRGGAGPGPGKPALEREGLRFLHVT 1
2 VGSLLATYGWYIVFSCILLYVVFQKLSTRLRALRQRHLDQAAAAL 1
2 EPDIVVKRQEALAAARLKMQEELNAQVEKHKEKLRQ 0
0 LEEEKQRRQKIEMWDSMQEGKSYKGNTRKPQ 0
0 EEDSPGPSTSSVIPKRKSDRKPLRGG 1
2 GYNPLSGEGGGTCSWRPGRRGPSSGGuG* 0 

>SELS_xenTro Xenopus tropicalis (frog) tga confirmed 
0 MELGNQPGPGNRPEIELEWYQYLQNT 1
2 VGVVLSSYGWYILLGCILIYLLIQKLPQNFTRAGTSNHSTVT 1
2 DPDEIVRRQEAVTAARLRMQEELNAQAELYKQKQVQ 0
0 LQEEKRQRNIETWDRMKEGKSSKVACRLGQEPS 0
0 PSTSTSAATKPKQEKQERKTLRGS 1
2 GYNPLTGDGGGTCAWRPGRRGPSSGGuG* 0

>SELS_danRer Danio rerio (zebrafish)
0 MEAEDGARVRNEDVPPQNQDLSFLQPS 1
2 VTAFMSEYGWYLLFGCVGVYLLIQHLRKSRSSTQTRSSSGSAEAH 1
2 DVGSVVRRQEALEASRRRMQEEQDARAAEFREKQRM 0
0 LEEEKRRQKIEMWDSMQEGKSYKGSAKVAQ 0
0 QNTEEAASSSSLRPKTEKKPLRSS 1
2 GYSPLSGDAGGSCSWRPGRRGPSAGGuG* 0

>SELS_calMil Callorhinchus milii (elephantfish) 
0 1
2 1
2 EPLLIVKQQEAMEAARRKMQAELDTQAVKYKEKQNL 0
0 LEEAKRRQKIEAWESIQEGRSYRANTRFNQ 0
0 QEPGQPSSSNLTKPKSGKKSLAT 1
2 GFNALTGDGGGSCTWRPNRRGPSSGGuG* 0

>SELS_petMar Petromyzon marinus (lamprey) cDNA EB082976 39% identity homSap tga-sel taa-stop full
0 MDPRNEEPAVIRGVSDAVSELLARFGWPL 1
2 LLFCALLYFTVRRAAPWLRWGRSSSSSTSISNP 1
2 DLLMNMHSAMERSRIRMQQELDARAAEHQARVKQ 0
0 LEEERQKQKMESWERGRSLRPRRDPQSQQ 0
0 EDNSTPSTSLPRTERQRLRDN 1
2 NYSPLSGGGGPTCLWRPGRRGPASGGGuG* 0

SELT: 11 vertebrate sequences

>SELT_homSap Homo sapiens (human) 5 exons recent pseudogenes on chr 5, chr9
0 MQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_macMul Macaca mulatta (rhesus)
0 MRLLLLLLVAASAMVRSEASANLGGVPSKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_otoGar Otolemur garnettii (bushbaby)
0 MRLLLLLLVAASAVVRSEASANLGGVPSKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIIGKIYSLHHSYR 2
1 HIASFLSIFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_musMus Mus musculus (mouse)
0 MRLLLLLLVAASAVVRSEASANLGGVPSKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_ratNor Rattus norvegicus (rat)
0 MRLLLLLLVAASAVVRSEASANLGGVPSKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_bosTau Bos taurus (cow)
0 MRLLLLLLVAASAVVRSDASANLGGVPGKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_canFam Canis familiaris (dog)
0 MRYLLVLLVAASAVIRSDASANLGGVPSKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_monDom Monodelphis domestica (opossum)
0 MRFLLLLLMAAAAL-QGEASADTGGVPGKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 IYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHH*SQ * 0

>SELT_ornAna Ornithorhynchus anatinus (platypus)
0 MRLLLLVVVAAAAGGRSEASADLGGLPSKRLKMQYATGPLLKFQIC 2
1 VSuGYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYR 2
1 HIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENK 0
0 VYACMMVFFLSNMIENQCMSTGAFEITLN 1
2 DVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS* 0

>SELT_petMar Petromyzon marinus (lamprey) cDNA EE737658 tga-sel tag-stop full
0 MRTHLFAGPVLKVEYC 2
1 VSuGYRRVFEEYSRVIAERFPDIRVEGDNYLPQPLYR 2
1 YIASFFSVFKLVLIGLVLSGKNLFPMLGVDTPGVWTWSQENK 0
0 LYACLMIFFVSNMVETQCMSTGAFEVSLN 1
2 DVPVWSKLQSGRVPSPQEILQILDNHVKLSGGSAGRMQPS* 0

>SELT_eptBur Eptatretus burgeri (hagfish) cdna BJ650136 tga-sel tag-stop full
0 MKSKMYSGPELLFQYC 2
1 ISuGYRRVFEEYSQALRERYPDIRIEGSNYPPPPLYS 2
1 TCASVLSVLKVMLIVLVVSGRNPFPLLGLDTPNAWNWSQNNK 0
0 IYACLMIFFLTNMIENQCLSTGAFEVVFN 1
2 DVPIWSKLQSGRVPSLPELAQILDNHLAMGGQASPSNTHGPQ* 0

SEPHS1: 13 chordate sequences

>SEPHS1_homSap Homo sapiens (human) PHYH- SEPHS1- DUF1172- PRFF18+ FRMD4A-
0 MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRL 1
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDR 0
0 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS* 0

>SEPHS1_musMus Mus musculus (mouse)
0 MSTRESFNPETYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRL 1
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDR 0
0 ERDKVIPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS* 0

>SEPHS1_loxAfr Loxodonta africana (elephant) frag
0 MSVRESFNPESYELDKSFRLTKFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGM 1
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGISNKMTDR 0
0 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2  1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTP 1

>SEPHS1_monDom Monodelphis domestica (opossum)
0 MSVRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRL 1
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGISNKMTDR 0
0 ERDKVVPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPEMAATALYTLDNNVLTLEQRQFYEDNGFLVIGATS* 0

>SEPHS1_ornAna Ornithorhynchus anatinus (platypus) virtual selenocys
0 MSVRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLEALQENHFQEDEQFLGAVMPRL 1
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGISNKMTDR 0
0 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS* 0

>SEPHS1_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01BJUY7 454
0                 AEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQ             0
2                             AKQQRSEVSFVIHNLPIIAKMAAITKACGNRFGLLQGTSSETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPRIIEVTPRGAAAPPPDNNSSA

>SEPHS1_galGal Gallus gallus (chicken)
0 MSvREtFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRL 1
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDR 0
0 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS* 0

>SEPHS1_xenTro Xenopus tropicalis (frog) frag
0 MSVRESFNPESYELDKSFRLTRFAELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRL
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKLTDR 0
0 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWVVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGSCPETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVASQNVNPTPGATS* 0

>SEPHS1_danRer Danio rerio (zebrafish) first occurence of threonine form
0 MSVRESFNPESYELDKNFRLTRFTELKGTGCKVPQDVLQKLLESLQENHYQEDEQFLGAVMPRL
2 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKLTEK 0
0 ERGKVMPLVIQGFKDASEEAGTSVTGGQTVINPWIVLGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMLNMARLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLARQQRTEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETS
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNTTPGATS* 0

>SEPHS1_calMil Callorhinchus milii (elephantfish) frag
0 MSVRETFNPENYELDKNFRLTRFAELKGTGCKVPQDVLHKLLEALQENHYQEDEQFLGAVMPRL 1
2 GIGMDSCVIPLRHGGLSLVQTTDFFYPLVDDPYMM 0
0 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMADK 0
0 ERDKVMPLIIQGFKDAADEAGTAVTGGQTVLNPWIILGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMFNMARLNRT 1
2 1
2 GGLLICLPREQAARFCAEIKSPKYGEGYQAWIIGIVEKGNRTARIIDKPRIIEVAPTVATQNVNPTPGATS*

>SEPHS1_petMar Petromyzon marinus (lamprey) cDNA LyEST8076 FD729689 tga-sel tgc-cys tag-stop not so virtual selenocys full
0 MSVHRYFDPEDHDLDKSFRLTKFSELKGuGCKVPQETLLKLLEGLEQDGPYQDEHQQFMGAVMPRL 1
2 GIGMDACVIPLRHGGLSLVQTTDFFYPLVDDPYMM 1
2 GKIACANVLSDLYAMGVTECDNMLMLLAISQKLSEK 0
0 ERDKVIPLMIRGFKDAAEEAGTTVTGGQTVVNPWIVIGGVATTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVNAHQWLDM 0
0 PEKWNKIKLVVTQEDVELAYQEAMFNMARLNRTAAGLMHTFNAH 2
1 AATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVIAKMAAISKACGNLFGLLQGTSAETSG 1
2 GLLICLPREQAARFCAEIaKYGEGHQAWIIGIVEKGGRTARIIDKPRIIEVAPRGASPTGVASPDTPTGPPLT* 0

>SEPHS1_eptBur Eptatretus burgeri (hagfish) cdna BJ649814 BJ654390 tga-sel taa-stop full
0 MSVHRYFDPEDHELDKSFRLTKFSDLKGuGCKVPQESLLKLLEGLEQDSPFQDEHQQFMGAVMPRL 1
2 GIGMDSCVIPLRHGGLSLVQTTDFFYPLVDDPYMM 0
0 GKIACANVLSDLYAMGVTDCDNMLMLLGISQKLSEK 0
0 ERDKVVPLMVRGFKDAAEEAGTTVTGGQTVMNPWIIIGGVASTVCQPNEFIM 2
1 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI 0
0 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAH 1
2 AATDITGFGILGHAQNLARQQRNEVAFVIHNLPRNEVAFVIHNLPVVAKMAAISKACGNLFGLLQGTSAETSG 1
2 GLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGARTARIIDKPRIIEVAPRGAPPPTVTTPTTPTAPLS* 0

>SEPHS1_braFlo Branchiostoma floridae (amphioxus) tga confirmed 73% hsa BW738890 BW748096 frag
0 MAAPEQVQQAVRPGRVFDPVAHGLDKSFRLTRFADLKuCCKVPQEVLLNLLEGLQNQTIPEEQGQFLPPHFAPI 1
2 GIGMDSCVVPLRQGGLSLVQTTDFFYPLVDDPYMQ 0
0 GKIACANVLSDLYAMGVKTCDNMLMLLGVSNKMSDK 0
0 ERDTVVPLIIRGFKDLAEEAGTMVQGGQTVLNPWVTIGGVATTVCPPNEFIE 2
1 PGNAVVGDVLVLTKPLGTQVAVNAHQWLDE 0
0 PERWNRIRLVVSEEDVERAYQESMFLMSKLNRT 1
2 AAKLMHKYNAHGATDITGFGLLGHAKNLVQHQKNEVSFTIHNLPVIAKMAAVAKACGNMFQLLQGYSAETS 1
2 * 0

SEPHS2: 8 vertebrate sequences

>SEPHS2_homSap Homo sapians (human) 1 exon but intronated like SEPHS1 for comparison 
MAEASATGACGEAMAAAEGSSGPAGLTLGRSFSNYRPFEPQALGLSPSWRLTGFSGMKGuGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPAL
GIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM
GRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEE
EKVTPLMVKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFIM
PDSAVVGDVLVLTKPLGTQVAVNAHQWLDN
PERWNKVKMVVSREEVELAYQEAMFNMATLNRT
AAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQGTSAETS
GGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAAVLAPDSSNASSEPSS*

>SEPHS2_musMus Mus musculus (mouse) 1 exon 
MAEAAAAGASGETMAALVAAEGSLGPAGWSAGRSFSNYRPFEPQTLGFSPSWRLTSFSGMKGuGCKVPQETLLKLLEGLTRPALQPPLTSGLVGGQEETVQEGGLSTRPGPGSAFPSL
SIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM
GRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEKER
EKVTPLMIKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQQNEFIM
PDSAVVGDVLVLTKPLGTQVAANAHQWLDN
PEKWNKIKMVVSREEVELAYQEAMFNMATLNRT
AAGLMHTFNAHAATDITGFGILGHSQNLAKQQKNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETS
GGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGASAAAAAAPDNSNAASEPSS*

>SEPHS2_loxAfr Loxodonta africanus (elephant) 1 exon
MAEAAATGASGEAMAVAEGSSGPAGFSLGRGFSSYRPFEPQALGLNPSWRLTGFSGMKGuGCKVPQETLLKLLAGLTRPDMRPPLGRTLVEGHEEAIQEAGLPAGSGPSPTLPSL
GIGLDSCVIPLRHGGLSLIQTTDFFYPLVEDPYMM
GRIACANVLSDLYAMGITECDNMLMLLSVSQNMSEE
EREKVTPLMIKGFRDAAEEGGTAVTGGQTVINPWIIIGGVATVVCQPNEFIM
PDSAVVGDVLVLTKPLGTQVAVNAHQWLDN
PERWNKIKMVVSREEVELAYQEAMFNMATLNRT
AAGLMHTFNAHAATDITGFGILGHSQNLARQQRNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETS
GGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGTTATTLVPDNFNASSEPTL*

>SEPHS2a_monDom Monodelphis domestica (opossum) processed 1 exon processed
MAAAAAATVGNGACTPATGGSLAGSYRPFEPQALGLSPNWRLTSFSDMKG uGCK VPQETLLTLLAGLTRPEERPPRAPGLNLGFGDVVAEEAAGPAVLEPGPDAGPFLPSAAGPSLPSPTL
GIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM
GRIACANVLSDLYAMGITECDNMLMLLSVSQKMNEE
EREKVMPLMIKGFRDAAEEGGTSVTGGQTVVNPWIIIGGVATVVCQPNEFIM
PDSAVPGDVLVLTKPLGTQVAVNAHQWLDN
PEKWNKIKLVVTREDVELAYQEAMFSMAMLNRT
AAGLMHTFNAHAATDVTGFGILGHAQNLAKQQRSQVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETS
GGLLICLPREHAARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPGIIEVTPRGATVPLHPNNSHASLEPGS*

>SEPHS2a_macEug Macropus eugenii (wallaby) 1 exon processed
MRRRQRTVRTEAVTPAVGGSFAGSYSPFEPQSLGLSPNRRLTSFSDMKGuGCKVPQEALLTLLAGLTRPEERPPRAPGLGLGFGDSAAEEAAGPAAPEPGPDAGPFLAPAAGASPSPPTL
GIGMDSCVIPLRHGGLSLVQTTDFFYPLMEDPYMM
GRIACANVLSDLYAMGITECDNMLMLLSVSQKMNEE
EKEKIMPLMIKGFRDAAEEGGTSVTGGQTVINPWIIIGGVATVVCQPNEFIM
PDSAVPGDVLVLTKPLGTQVAVNAHQWLDNPERWNKIKLVVTREDVELAYQEAMFNMAMLNRT
AAGLMHTFNAHAATDVTGFGILGHAQNLAKQQRSQVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETS
GGLLICLPREHAARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPGIIEVTPRGATVPLHPNNSHASLEPGS* 0

>SEPHS2b_monDom Monodelphis domestica (opossum) unprocessed 8 exons no syntenty to 1 frag
0 MASVPPRAPAAEGAGAASPGAYRPFEPKALGLSPSWRLTGFSELKGuGCKVPPEALLTLLASLTAAPAPAADRGN 1
2 GIGMDSCVVPLRHGGLSLVQTTDFFYPLVEDPYMM 0
0 GRIACANVLSDLYAMGITECDNMLMLLSVSQKMSEE 0
0 EREKIMPLMVRGFRDAAEQGGTSVTGGHTVINPWLIIGGVATSVCQPNEFIM 2
1  0
0 PEKWNKIKLVVSKEDVELAYQEAMFSMAMLNRT 1
2 AARLMHTFNAHAATDITGFGILGHAQNLAKQQRSEVSFVIHNLPIIAKMAAVSKASGCFGLLQGTSAETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPRIIEVMPRGATLHQDNSNTSPEPAL* 0

>SEPHS2b_macEug Macropus eugenii (wallaby) unprocessed
0 MAAAPPPPPPPLAAEGASAGAGARSQGAYRPFEPQALGLSPSWRLTGFSELKGuGCKVPQEALLTLLAGLARPPVAPAPDGGD 1
2 GIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM 0
0 GRIACANVLSDLYAMGITDCDNMLMLLSISQKMNEE 0
0 EREKIMPLMVKGFRDAAEEGGTSVTGGHTVINPWIIIGGVATVVCQSNEFIM 2
1  0
0 PERWNKIKLVVSKEDVELAYQEAMFSMAMLNRT 1
2 AARLMHTFNAHAATDITGFGILGHAQNLAKQQRSEVSFVIHNLPIIAKMAAVSKASGCFGLLQGTSAETS 1
2 GGLLICLPREQ AARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPRIIEVMPRGAALHHDSSNTSPEPAS* 0

>SEPHS2_ornAna Ornithorhynchus anatinus (platypus) tga confirmed
0                 FDPELLGLSPSWRLTGFSELKGuGCKVPQETLLKLLAGLTHPDPRPGAAPPDPQDPPPDPQDRTPHHAGPAPPAL 1
2 GIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM 0
0 GRIACANVLSDLYALGITECDNMLMLLSVSQKMNEE 0
0 EREKIMPLMIKGFRDAAEEGGTSVTGGQTVVNPWIIIGGVATVVCQPNEFIM 2
0 PDNAVPGDVLVLTKPLGTQVAVNAHQWLDN 0
0 PEKWNKIKLVVSREDVELAYQEAMFSMAMLNRT 1
2 AAGLMHTFNAHAATDITGFGILGHAQNLAKQQRSEVSFVIHNLPIIAKMAAITKACGNRFGLLQGTSSETS 1
2 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNQTARIIDKPRIIEVTPRGAAAPPPDNNSSASPETGS* 0

>SEPHS2_tacAcu Tachyglossus aculeatus (echidna) EUPZL4S02I1W5B 454
0           KLVVSREDVELAYQEAMFSMAMLNRT 1
2 AAGLMHTFNAHAATDI

>SEPHS2_galGal Gallus gallus (chicken) frag
0 MAAPPGPSVPSPTPGPSPGSGCALGPSTSYRPFDPTSLGLDPNWRLTSYSELRGuGCKVPEETLLRLLEGLGGPRSGAAAAAAAAGGGDGEGAEPGGAPTL 1
2 GIGTDCCVIPLRHGGLSLVQTTDFFYPLVDDPYMM 0
0 GRIACANVLSDLYAMGITECDNMLMLLSVSQKMTDE 0
0 ERDKVMPLIVRGFRDAAEAAGTSVTGGQTVLNPWVIVGGAASVVCQRQEFIV 2
1 PDSAVPGDVLVLTKPLGTQVAVTAHQWLDN 0
0 PERWNKIKLVVTREEVEAAYREAMFSMATLNRT 1
2 AAGLMHTFNAHAATDITGFGILGMHKNLAKQQRNEVSLLFTSLPVLAKNGCCQMAAVSKGLWQYCLGLYSLELVPETS 1
2 GGLLICLPREQAARFCAE

>SEPHS2_xenTro Xenopus tropicalis (frog) 8 exons in genome
0 maAPGASHLSAACPFFPGYRPFDPVSVGLEASFRLTSFSDLKGuGCKVPRETLLRLLTGLTEEEAAVSGGAIRDPDVPCAGQDGGQNRL 1
2 GIGLDSCVIPLRHRGLSLVQTTDFFYPSVEDPYMQ 0
0 GRIACANVLSDLYAMGITECDNMLMLLSVSQKMTEE 0
0 EREKVTPLMIKGFRDAAEEGGTSVTGGQTVVNPWIIIGGVATVVCQANEFIM 2
1 PQNAVAGDVLVLTKPLGTQVAVNAHQWLDN 0
0 PERWNKIRLVVSREDVELAYQEAHVNMATLNRT 1
2 AAALMHSFNAHAATDVTGFGILGHAQNLAQEQQNEVSFVIHNLPIIAKAAITKACGNRFgLLQGTSPETS 1
2 GGLLICLPREQAARFCAEIKSPKHGEGHQAWIIGIVEKGNRSARIIEKPRiiEVTPRGAaTSDNTA* 0

TXNRD1 (TR1): 27 chordate terminal exons

>TXNRD1_homSap Homo sapiens (human) 13 exons tga-sel taa-stop
0 MNGPEDLPKSYDYDLIIIGGGSGGLAAAK 0
0 EAAQYGKKVMVLDFVTPTPLGTRW 1
2 GLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEET 1
2 VKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIK 0
0 ATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISS 0
0 DDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIK 0
0 VEQIEAGTPGRLRVVAQSTNSEEIIEGEYNT 0
0 VMLAIGRDACTRKIGLETVGVKINEK 2
1 TGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVK 0
0 CDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIE 0
0 VYHSYFWPLEWTIPSRDNNKCYAKIICNTKDN 0
0 ERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAE 0
0 VFTTLSVTKRSGASILQAGCuG* 0

VFTTLSVTKRSGASILQAGCuG TXNRD1_homSap Homo sapiens (human)
VFTTLSVTKRSGASILQAGCuG TXNRD1_panTro Pan troglodytes (chimp)
VFTTLSVTKRSGASILQAGCuG TXNRD1_ponPyg Pongo pygmaeus (orang_sumatran)
VFTTLSVTKRSGASILQAGCuG TXNRD1_macMul Macaca mulatta (rhesus)
VFTTLSVTKRSGGSVLQAGCuG TXNRD1_otoGar Otolemur garnettii (bushbaby)
VFTTLSVTKRSGGSILQAGCuG TXNRD1_tupBel Tupaia belangeri (tree_shrew)
IFTTLSVTKRSGGDILQSGCuG TXNRD1_musMus Mus musculus (mouse)
IFTTLSVTKRSGGDILQSGCuG TXNRD1_ratNor Rattus norvegicus (rat)
VFTTLSVTKRSGASVLQSGCuG TXNRD1_cavPor Cavia porcellus (guinea_pig)
VFTTLSVTKRSGGDILQAGCuG TXNRD1_oryCun Oryctolagus cuniculus (rabbit)
VFTTLSVTKRSGASILQAGCuG TXNRD1_canFam Canis familiaris (dog)
IFTTLSVTKRSGGNILQTGCuG TXNRD1_equCab Equus caballus (horse)
VFTTLSVTKRSGGNILQTGCuG TXNRD1_bosTau Bos taurus (cow)
VFTTLSVTKRSGASILQAGCuG TXNRD1_susScr Sus scrofa (pig)
VFTTLSVTKRSGGSILQAGCuG TXNRD1_eriEur Erinaceus europaeus (hedgehog)
VFTTLSVTKRSGGSILQTGCuG TXNRD1_echTel Echinops telfairi (tenrec)
VFTTLSVTKRSGGSILQAGCuG TXNRD1_monDom Monodelphis domestica (opossum)
IFTTLTVTKRSGGNVIQAGCuG TXNRD1_ornAna Ornithorhynchus anatinus (platypus)
VFTTLSITKRSGENTLQSGCuG TXNRD1_galGal Gallus gallus (chicken)
IFTTLSVTKRSGENILQSGCuG TXNRD1_anoCar Anolis carolinensis (lizard)
IFTTLTVTKRSGGNILQSGCuG TXNRD1_xenTro Xenopus tropicalis (frog)
IFTTMEVTKSSGGDITQSGCuG TXNRD1_danRer Danio rerio (zebrafish)
IFTTLEVTKSSGKSIIQTGCuG TXNRD1_tetNig Tetraodon nigroviridis (pufferfish)
IFTTMEVTKSSGGNINQSGCuG TXNRD1_takRub Takifugu rubripes (fugu)
VFTTLDVTKRSGGNITQSGCuG TXNRD1_gasAcu Gasterosteus  aculeatus (stickleback)
IFTTLQVTKSSGGDIKQAGCuG TXNRD1_oryLap Oryzias latipes (medaka)
IFTMLAVTKRSGADIKQTGCuG TXNRD1_petMar Petromyzon marinus (lamprey)
IFTTMDITKGSGEDPTKTGCuG TXNRD1_braFlo Branchiostoma floridae (amphioxus)

TXNRD3 (TR3): 29 chordate terminal exons

>TXNRD3_homSap Homo sapiens (human) 12 exons tga-sel tga-stop
0 MVLDFVVPSPQGTSW 1
2 GLGGTCVNVGCIPKKLMHQAALLGQALCDSRKFGWEYNQQ 1
2 VRHNWETMTKAIQNHISSLNWGYRLSLREKAVAYVNSYGEFVEHIK 0
0 ATNKKGQETYYTAAQFVIATGERPRYLGIQGDKEYCITS 2
1 DDLFSLPYCPGKTLVVGASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYMEQHGVKFLRKFIPVM 0
0 VQQLEKGSPGKLKVLAKSTEGTETIEGVYNT 0
0 VLLAIGRDSCTRKIGLEKIGVKINEK 2
1 SGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLFGASLEK 0
0 CDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLE 0
0 IYHTLFWPLEWTVAGRENNTCYAKIICNKFDH 0
0 DRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGE 0
0 VFTTLEITKSSGLDITQKGCuG* 0

VFTTLEITKSSGLDITQKGCuG TXNRD3_homSap Homo sapiens (human)
VFTTLEITKSSGLDITQKGCuG TXNRD3_panTro Pan troglodytes (chimp)
VFTTLEITKSSGLDITQKGCuG TXNRD3_macMul Macaca mulatta (rhesus)
VFTTLEITKSSGLDITQKGCuG TXNRD3_musMus Mus musculus (mouse)
VFTTMEITKSSGLDITQKGCuG TXNRD3_ratNor Rattus norvegicus (rat)
VFTTLDITKSSGLDITQRGCuG TXNRD3_cavPor Cavia porcellus (guinea_pig)
VFTTLEITKSSGLAVTQQGCuG TXNRD3_oryCun Oryctolagus cuniculus (rabbit)
VFTTLEITKSSGLDITQKGCuG TXNRD3_canFam Canis familiaris (dog)
VFTTLEITKSSGLDITQKGCuG TXNRD3_felCat Felis catus (cat)
VFTTLEITKASGLDITQKGCuG TXNRD3_equCab Equus caballus (horse)
VFTTLEITKSSGLDITQKGCuG TXNRD3_myoLuc Myotis lucifugus (microbat)
VFTTLEITKASGLDITQKGCuG TXNRD3_bosTau Bos taurus (cow)
IFTTLEITKASGLDILQRGCuG TXNRD3_sorAra Sorex araneus (shrew)
VFTTLEITKSSGLDITQKGCuG TXNRD3_dasNov Dasypus novemcinctus (armadillo)
VFTTLEITKSSGLDITQKGCuA TXNRD3_loxAfr Loxodonta africana (elephant) A=single trace 
VFTTLEITKSSGLDITQKGCuG TXNRD3_proCap Procavia capensis (hyrax)
VFTSLEITKSSGLDISQKGCuG TXNRD3_echTel Echinops telfairi (tenrec)
VFTTLEITKSSRTVISPRGCuG TXNRD3_monDom Monodelphis domestica (opossum)
IFTTLTVTKRSGGNVIQAGCuG TXNRD3_ornAna Ornithorhynchus anatinus (platypus)
VFTTMDITKSSGQDITQRGCuG TXNRD3_galGal Gallus gallus (chicken)
VFTTMDITRASGKDIAQSGCuG TXNRD3_anoCar Anolis carolinensis (lizard)
VFTTMDVSKSSGGDITQKGCuG TXNRD3_xenTro Xenopus tropicalis (frog)
IFTTMEVTKSSGGDITQSGCuG TXNRD3_danRer Danio rerio (zebrafish)
IFTTLEVTKSSGKSIIQTGCuG TXNRD3_tetNig Tetraodon nigroviridis (pufferfish)
IFTTMEVTKSSGGNINQSGCuG TXNRD3_takRub Takifugu rubripes (fugu)
VFTTLDVTKRSGGNITQSGCuG TXNRD3_gasAcu Gasterosteus  aculeatus (stickleback)
IFTTLQVTKSSGGDIKQAGCuG TXNRD3_oryLap Oryzias latipes (medaka)
VFTTLSVSKRSGGNIQQSGCuG TXNRD3_calMil Callorhinchus milii (elephantfish)
IFTMLAVTKRSGADIKQTGCuG TXNRD3_petMar Petromyzon marinus (lamprey)
IFTTMDITKGSGEDPTKTGCuG TXNRD3_braFlo Branchiostoma floridae (amphioxus)

TXNRD2 (TGR): 33 vertebrate terminal GCUG* exons

>TXNRD2_homSap Homo sapiens (human) 17 exons tga-sel taa-stop
0 MAAMAVALRGLGGRFRWRTQAVAGGVRGAARGAA 1
2 AGQRDYDLLVVGGGSGGLACAKE 1
2 AAQLGRKVAVVDYVEPSPQ 1
2 GTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPVPHDW 2
1 RKMAEAVQNHVKSLNWGHRVQLQDR 2
1 KVKYFNIKASFVDEHTVCGVAKGGKE 0
0 ILLSADHIIIATGGRPRYPTH 0
0 IEGALEYGITSDDIFWLKESPGKT 2
1 LVVGAS 1
2 YVALECAGFLTGIGLDTTIMMRSIPLRGFDQ 0
0 QMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAI 1
2 GRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVE 0
0 GRPELTPIAIMAGRLLVQRLFGGSSDLMDYDN 0
0 VPTTVFTPLEYGCVGLSEEEAVARHGQEHVE 0
0 VYHAHYKPLEFTVAGRDASQCYVK 0
0 MVCLREPPQLVLGLHFLGPNAGEVTQGFALGIK 2
1 CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0

1 CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_homSap Homo sapiens (human)
1 CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_ponPyg Pongo pygmaeus (orang_sumatran)
1 CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_macMul Macaca mulatta (rhesus)
1 CGASYVQVMQTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_calJac Callithrix jacchus (marmoset)
1 CGASYAQVMRTVGIHPTCSEEVVRLRISKRSGLDPTVTGCuG* 0  TXNRD2_otoGar Otolemur garnettii (bushbaby)
1 CGASYAQVTRTVGIHPTCAEEVVRLRISKRSGLDPTVTGCuG* 0  TXNRD2_micMur Microcebus murinus (mouse_lemur)
1 CGASYAQVMQTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_tupBel Tupaia belangeri (tree_shrew)
1 CGASYAQVMQTVGIHPTCSEEVVKLHISKRSGLEPTVTGCuG* 0  TXNRD2_musMus Mus musculus (mouse)
1 CGASYAQVMQTVGIHPTCSEEVVKLHISKRSGLDPTVTGCuG* 0  TXNRD2_ratNor Rattus norvegicus (rat)
1 CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_cavPor Cavia porcellus (guinea_pig)
1 CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_ochPri Ochotona princeps (pika)
1 CGASYAQVMRTVGIHPTCAEEVAKLRITKRSGLDPTVTGCuG* 0  TXNRD2_canFam Canis familiaris (dog)
1 CGASYAQVMRTLGIHPTCAEEVAKLRITKRSGLDPTVTGCuG* 0  TXNRD2_equCab Equus caballus (horse)
1 CGASYAQVMRTVGIHPTCAEEVTKLRISKRSGLDPTVTGCuG* 0  TXNRD2_myoLuc Myotis lucifugus (microbat)
1 CGASYQQLMRTVGIHPTCAEEVAKLRISKRSGLDPTVTGCuG* 0  TXNRD2_bosTau Bos taurus (cow)
1 CGASFQQVIRTVGIHPTCAEEVAKLRISKRSGLDPTVTGCuG* 0  TXNRD2_oviAri Ovis aries (sheep)
1 CGASYEQVMRTVGIHPTCAEEVAKLRISKRSGLDPTVTGCuG* 0  TXNRD2_susScr Sus scrofa (pig)
1 CGASYTQVMQTVGIHPTCAEEVAKLRISKRSGLDPTVTGCuG* 0  TXNRD2_eriEur Erinaceus europaeus (hedgehog)
1 CGASYAQVMQTVGIHPTCAEEVAKLHISKRSGLDPTVTGCuG* 0  TXNRD2_sorAra Sorex araneus (shrew)
1 CGASYAQLMQTVGIHPTCAEEVAKLHISKRSGQDPTVTGCuG* 0  TXNRD2_dasNov Dasypus novemcinctus (armadillo)
1 CGASYAQMMRTVGIHPTCAEEVVKLRISKRSGLDPTVTGCuG* 0  TXNRD2_loxAfr Loxodonta africana (elephant)
1 CGATYSDLMKTVGIHPTCAEEVTKLKITKRSGLSAVITGCuG* 0  TXNRD2_monDom Monodelphis domestica (opossum)
1 CGATYPQMKKTVGIHPTSAEEVTKLWITKRSGLPATVTSCuG* 0  TXNRD2_macEug Macropus eugenii (wallaby)
1 CGATYSQMMKTVGIHPTCAEEVTKLHITKRSGLDPTVTGCuG* 0  TXNRD2_galGal Gallus gallus (chicken)
1 CGATYSQMMKTVGIHPTCAEEVTKLHITKRSGLDPTVTGCuG* 0  TXNRD2_anoCar Anolis carolinensis (lizard)
1 CGATYHQLMRTVGIHPTCAEEVTKLHITKRSGLDPTVTGCuG* 0  TXNRD2_xenTro Xenopus tropicalis (frog)
1 CGLTYEHLRNTVGIHPTCAEELTKLNITKRSGLDATVTGCuG* 0  TXNRD2_danRer Danio rerio (zebrafish)
1 CGATYSHLLQTVGIHPTCAEEVIKVNITKRSGLDATVTGCuG* 0  TXNRD2_tetNig Tetraodon nigroviridis (pufferfish)
1 CGATYSHLLQTVGIHPTCAEEVIKVNITKRSGLDATVTGCuG* 0  TXNRD2_takRub Takifugu rubripes (fugu)
1 CGATYSHLLQTVGIHPTCAEEVVKVNITKRSGLDPAVTGCuG* 0  TXNRD2_gasAcu Gasterosteus  aculeatus (stickleback)
1 CGATYRQLMQTVGIHPTSAEELVKINITKRSGLDPTVTGCuG* 0  TXNRD2_oryLap Oryzias latipes (medaka)
1 CEATYSQLVATVGIHPTCAEDVNKLNITKRSRLDPTVTGCuG* 0  TXNRD2_calMil Callorhinchus milii (elephantfish)
1 CNATYSQLASTVGIHPTCAEDVNKLNITKRSGQDATVTGCuG* 0  TXNRD2_squAca Squalus acanthias (spiny_dogfish)

 CLRVQIFTMLAVTKRSGADIKQTGCLAVTKRSGADIKQTGCuG* 0  TXNRD2_petMar Petromyzon marinus (lamprey)*
  SGLTYQQLASSVGIHPTCAEEVVKMGITKRSGLDPTVTGCuG* 0  TXNRD2_braFlo Branchiostoma floridae (amphioxus)*

*may not be orthologs: TXNRD2_braFlo has last 3 exons fused though correct phase 0
0 IYHAFYKPLEYYIPERDASQCYIKAVCKRVGDQEIVGLHFLGPNAGEVTQGFAVALRSGLTYQQLASSVGIHPTCAEEVVKMGITKRSGLDPTVTGCuG* 0

not available TXNRD2_ornAna Ornithorhynchus anatinus (platypus)

SEPP1 (SELP): 15 vertebrate sequences

>SEPP1_homSap Homo sapiens (human) chr5 4 exons 10 selenocysteines
0 MWRSLGLALALCLLPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSNGSVTVVALLQASuYLCILQASK 2
1 LEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLNGSKDDFLIYDR 2
1 CGRLVYHLGLPFSFLTFPYVEEAIKIAYCEKKCGNCSLT 0
0 TLKDEDFCKRVSLATVDKTVETPSPHYHHEHHHNHGHQHLGSSELSENQQPGAPNAPTHPAPPGLHHHHKHKGQHRQGH
PENRDMPASEDLQDLQKKLCRKRCINQLLCKLPTDSELAPRSuCCHCRHLIFEKTGSAITuQCKENLPSLCSuQGLRAEEN
ITESCQuRLPPAAuQISQQLIPTEASASuRuKNQAKKuEuPSN* 0

>SEPP1_panTro Pan troglodytes (chimp)
0 MWRSLGLALALCLLPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSSGSVTVVALLQASuYLCILQASK 2
1 LEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLNGSKDDFLIYDR 2
1 CGRLVYHLGLPFSFLTFPYVEEAIKIAYCEKKCGNCSLT 0
0 TLKDEDFCKRVSLATVDKTVETPSPHYHHEHHHNHGHQHLGSSELSENQQPGAPNAPTHPAPPGLHHHHKHKGQH
RQGHPENQDMPGSEDLQDLQKKLCRKRCINQLLCKLPKDSELAPRSCCCHCRHLIFEKTGSAITuQCKENLPSLCSu
QGLRAEENITESCQuRLPPAAuQISQQLIPTEASTSuCuKNQAKKuEuPSN* 0

>SEPP1_ponPyg Pongo pygmaeus (orang_sumatran)
0 MWRSLGLALALCLLPLGGTESQDQSSLCKQPPAWSIRDQGPMLNSNGSVTVVALLQASuYLCILQASK 2
1 LEDLRVKLKKEGYSNISHIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLNGSKDDFLIYDR 2
1 CGRLVYHLGLPFSFLTFPYVEEAVKIAYCEKKCGYCSLT 0
0 TLKDEDFCKSVSLATVDKTVEAPSPHYHHEHHHNHRHQHLGSSELSENQQPGAPDAPTHPAPPGLHHHHKHKGQH
RQGHPENRDMPGSEDIQDLQKKLCRKRCINQLLCKLPKDSELAPRSCCCHCRHLIFEKTGSAITuQCKENLPSLCSu
QGLRAEENITESCQuRLPPPAuQISQQLIPTEASTSuRuKNQAKKuEuPSN* 0

>SEPP1_macMul Macaca mulatta (rhesus)
0 MWRSLGLALALCLLPSGGTESQDQSSFCKQPPAWSIRDQDPMLDSNGSVTVVALLQASuYLCILQASK 2
1 LEELRVKLEKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLNGSKDDFLIYDR 2
1 CGLLVYHLGLPFSFLTLPYVEEAIKIVYCEKKCGNCSLT 0
0 TLKDEDFCKSVSLATVDETVEAPQAHYHHEHHHNQGHQSSELSENQQPGAPGAPTHPAPPGLHHHHKHKGQHRQGH
PESuDMPGSEGLQHLQKKLuRKRCINQLLCKLPKDSELAPRSuCCHCRHLIFEKTGSAITuQCKENLPSLCSuQGLLAEET* 0

>SEPP1_musMus Mus musculus (mouse)
0 MWRSLGLALALCLLPYGGAESQGQSSACYKAPEWYIGDQNPMLNSEGKVTVVALLQASuYLCLLQASR 2
1 LEDLRIKLESQGYFNISYIVVNHQGSPSQLKHSHLKKQVSEHIAVYRQEEDGIDVWTLLNGNKDDFLIYDR 2
1 CGRLVYHLGLPYSFLTFPYVEEAIKIAYCEERCGNCNLT 0
0 SLEDEDFCKTVTSATANKTAEPSEAHSHHKHHNKHGQEHLGSSKPSENQQPGPSETTLPPSGLHHHHRHRGQHRQGH
LESuDTTASEGLHLSLAQRKLuRRGCINQLLCKLSKESEAAPSSCCCHCRHLIFEKSGSAIAuQCuENLPSLCSuQGLF
AEEKVTESCQCRSPPAAuQNQPMNPMEANPNuSuDNQTRKuKuHSN* 0

>SEPP1_ratNor Rattus norvegicus (rat)
0 MWRSLGLALALCLLPYGGAESQGQSPACKQAPPWNIGDQNPMLNSEGTVTVVALLQASuYLCLLQASR  2
1 LEDLRIKLENQGYFNISYIVVNHQGSPSQLKHAHLKKQVSDHIAVYRQDEHQTDVWTLLNGNKDDFLIYDR 2
1 CGRLVYHLGLPYSFLTFPYVEEAIKIAYCEKRCGNCSFT 0
0 SLEDEAFCKNVSSATASKTTEPSEEHNHHKHHDKHGHEHLGSSKPSENQQPGALDVETSLPPSGLHHHHHHHKHKGQH
RQGHLESuDMGASEGLQLSLAQRKLuRRGCINQLLCKLSEESGAATSSCCCHCRHLIFEKSGSAITuQCAENLPSLCSuQ
GLFAEEKVIESCQCRSPPAAuHSQHVSPTEASPNuSuNNKTKKuKuNLN* 0

>SEPP1_canFam Canis familiaris (dog)
0 MWRSLGLALALCLLPWGGAESQGQSSFCKQPPAWSIRDQNPMLNSSGSVTVVALLQASuYLCILQASR 2
1 LEDLRVKLEKEGFLNISYVVVNHQGLSSQLKYMYLKNKVSEHIPVYQQEENQTDVWTLLNGKKDDFLIYDR 2
1 CGRLVYHLGLPYSFLTFPYVEEAIKRAYCEEKCGNCSLT 0
0 VLEDEEVCKMVSSGTVESTTEAPQPHPHDHHLHHHHHHHHKHWHRLMPHGNDELSENQQPEEPDVSEHPAPQGLHRHHKHKDHQRQGH
PDNuDMPAGSESLQLSVPQNQLuRKGCRNQLLCKLPRDSGLAPSSuCuHuRHLIFEKTGSAITuQCKETLPSLCSuQGLWAEENVIESuQ
uRWPPAAuQASQQLRPTEASTNuSuKYKTKMuKuLTY* 0

>SEPP1_equCab Equus caballus (horse)
0 MWRSLGLALALCLLPWGGTESQGQSSFCKQPPAWSIRDQDPMLNSYGSVTVVALLQASuYLCLLQASR 2
1 LEDLRVKLEKEGYSNISYVVVNHQEISARLKYIHLKNKVSEYIAVYQQEENQTDIWTLLNGSKDDFLIYDR 2
1 CGRLVYHLGLPYSFLTFPYVEEAIKIAYCEKKCGNCSLM 0
0 TLEDEDFCKTLSLATVEKTTEDSQPHHHHHHQHHHKHGHQHVGNSQLSENQQPEATGAPEHPPPPGLHHHHKHKGQQ
RRGHPENuDMPGGESLQLSLPQKKLuQKGCINQLLCKMPKDSKLAPSSuCuHCRHLIFENTRSAITuQCTENLPSLCSuQ
GLWAEENVIESCQuRLPPAAuQPS-QQLKPTEASTNuSuKYKAAMuKuPSN* 0

>SEPP1_bosTau Bos taurus (cow) 12 sel
0 MWRGLGLALALCLLLTGGTESQGQSSYCKQPPPWSIKDQDPMLNSYGSVTVVALLQASuYLCILQASR 2
1 LEDLRVKLEKEGYSNISYVVVNHQGISSRLKYVHLKNKVSEHIPVYQQEENQPDVWTLLNGNKDDFLIYDR 2
1 CGRLVYHLGLPYSFLTFTYVEDSIKTVYCEDKCGNCSLS 0
0 RPQDEDVCKNVFLATKEKTAEASQRHHHPHPHSHPHPHPHPHPHPHPHPHHGHQLHENAHLSESPKPDTPDTPENPPTSGLHHHHHRHKGPQRQGH
SDNCDTPLGSESLQPSLPQKKLuRKRCINQLLuQFPKYSESALSSCCCHCRHLVFEKTGSAITuQCTEKLPSLCSuQGLLAEENVIESuQuRLPPAAu
QAAGQQLNPTEASTKuSuKNKAKMuKuPSN* 0

>SEPP1_choHof Choloepus hoffmanni (sloth)
0 MWKSLGLALALCLLPWGGTESQEQSPFCKRPPAWTIRGQDPMLNSNGSVTVVALLQASuYLCILQASR 2
1 LEDLRVKLEKEGYSNISYIIVNHHEITSQLKYIHLKNKVSEHISVYQQKENERDVWALLNGKKDDFFIYDR 2
1 CGHLVYHFRLPYSFLTFPYVEEGIKIAYCENKCGNCPPT 0
0  FINLFQALKDEDVCENVSLAITEKTTEVSQPHPHHNHHHKHPGNSQLPENQQTGAPDAPQQPPPPGLHHHHKHQGQQREGHPDNCDMPESEGLQLSPHQKKLuRKRCINQLFCKVSKDSE
LAPRSCCuHCRHLVFEKTGSAITCQCKENLPSLCSuQGLWAEENVIESuQuRLPPAAuQISQQLLKPTEVSTNuEuNNKADMuKuPAN* 0

>SEPP1_ornAna Ornithorhynchus anatinus (platypus)
0 MWQGLGLALALCLLPGGGAESQSASSHCKEAPRWQIRDQDPMLNSLGTVTVVALLQASuYLCILQASR 2
1 LEDLRVKLENEGYSNISYIIVNHQGMPSQLNHKTLKEKVSEHIPVYQQDEKQTDVWSTLKGNKDDFLIYDR 2
1 CGRLVYHLSLPYTFLSFSYVEDSIKTTYCEQNCGNCSYT 0
0 MPEAEEFCTNTSSAAKEKATEAPLPHNDRPHHHHHHHHHHGHKPHPSGTEQAPADPDGPLRSPAPQGLHKRLRPAGQPRQGQ
GGSREAAEGRGEELPSPRKKAuRKGNASCQNQLLuDWHKRSGPAPSSuCuHCRHLLFGSKATATALuQCRDALPALCSuQGRQS
GEDVIESuQuRSPLPAuPPAAQLPSPSPTDPNAAuKuENTAGMuKuPTR* 0

>SEPP1_galGal Gallus gallus (chicken) reqd traces tga-sel taa-stop
0 MWAGLGLALVLCLLPGGGTESQRCQEPPEWHIGEESPMLNARGSVAVVALLQASuYLCLLQASR 2
1 LEDLRVKLENEGLVNISYVVVNHQSPHSQKKFHLLQESVSDHITVYQQDDHQADVWTTLNGNKDDFLIYDR 2
1 CGRLVYHLGLPYSFLSFQYVEEAIKIAYCENNCGNCSYT 0
0 EPDIDNICENITKKEDENLAGIEPEPEPSGQHSHHHHQLHRHRHHHHHREGGRHSKNQNHQAPSESQRRHPHNGRRHRVFNHNRHDQ
IGSHEQVETLPPGEGVENLPRVTKLuKKGKTICKNQLTuNWQTASDSTTSSuCCHCRHLLFEELGNSITuQ
CRGALPNSCRuHGQLLAEDITESuQuRLLTAAuESAAGGGSETSDTuLuERAGNuAuKTN* 0

>SEPP1_xenTro Xenopus tropicalis (frog) frag
0 MWKGFALALALCLLPWGGAESQGHRKRCKEPPEWSIGDQNPMMQSAGKVTVVALLQASuSLCLLQASR 2
1 LEDLRLKLEKEKLVGISYVVVNHQGRQSRAKYDLLKSKVSEHIPVYQQEEYQPDVWSLLKGDKDDFFVYDR 2
1 CGRLVQHLELPYSLLHFPYVEEAIRIAYCEDKCGECEHK 0
0 ILDADVCKKPEEQQEQDKPVEEKVERPRHHRNHHRHHRPKHSGHRHRHHHSEDGQVAELDVLRSSAQANNRAGSQNGQGQ
SVVQVVPQSEVLFVPQREADIPVLARQPuKKAKSuKKQYLuDWREEGGKAFNSuCuHuRQLSFEVTENEVAuHuQEALPSSC
SuQELLSDSLPESuQuRLSAAAuHSESTGLPETKLESEPNAPuAuPQEAENuQuKELuRFLM* 0

>SEPP1_xenTro Xenopus laevis (frog)
0 MWKGFALALALCLLPWGGAESQGHRSRCKQPPDWSIGDQNPMIQSAGKVTVVALLQASuYLCLLQASR 2
1 LEDLRLKLEKEKLVGISYVVVNHQGRHSRAKYDLLKSKVSEHIPVYQQEENQPDVWSLLKGDKDDFFIYDR 2
1 CGRLVQHLELPYSLLHFPYVEEAVRIAYCGDKCGECEHK 0
0 IPDADVCKKPEEQPELEKPVEEKVERPRPHRNHHRHHRPKHSGHRHRHHNNEGGQAAEVDAFQTNNRAGSHNGQGQSVVPQSEVVFVPQ
READVPVLALQPuKKAKSuKKQYLuEWREDAGKAFNSuCuHuRQLSFEIAQNEVTuRCQEALPASuQuQELLSDSLSESuQuRLSAAAuHSHS
TGLPELDTESETNAPuAuPQEAENuQuKELuRFLM* 0

>SEPP1_danRer Danio rerio (zebrafish) 17 sel
0 MWKALSLTLALCLLVGCSAESETEGARCKLPPEWKVGDVEPMKNALGQVTVVAYLQASuLFCLEQASK 2
1 LNDLLLKLENQGYPNIAYMVVNNREERSQRLHHLLQERLLNITLYAQDLSQPDAWQAVNAEKDDILVYDR 2
1 CGRLTYHLSLPYTILSHPHVEEAIKHTYCDRICGECSLES 0
0 SAQLEECKKATEEVNKPVEEEPRQDHGHHEHGHHEHQGEAERHRHGHHHPHHHHHHHRGQQQVDVDQQVLSQVDFGQVAVETPMMKRPuAKHSRuKVQYSuQQGADSPVASu
CuHuRQLFGGEGNGRVAGLuHCDEPLPASuPuQGLKEQDNHIKETuQuRPAPPAEuELSQPTuVuPAGDATuGuRKK* 0

>SEPP1_torCal Torpedo californica (electric_ray) 1 tga-sel taa-stop
0 MQQGLGVALALCLLLVGQAESQGQCDKPSEWTIGDKNPMKDSLGKVTVLALFQASuHFCLEQAGS 2
1 LKNLKRKLDQDGLEHISYMVVNHQGNDSRKRYQELKRNVQGKLPVYQQEIDQPDVWNLLQGQKDDFFIYDR 2
1 CGQQTFHLELPFTILSQMYVEHAIKQTYCQEICTNCS 0
0 LAELPFGMQQHKMQKSQRRWEEDGSPVPKHRGSTMGHKTTPPQSPR*

SEPP2 (SELPB): 16 vertebrate sequences

>SEPP2_monDom Monodelphis domestica (opossum) chr2:9,451,774-9,455,977 1 selenocys tga stop taa
0 MPPPGLSLAVLLGLLGASLAFENRTRFCQPAPPWQVGGGRAPMEEALGNVTVVALLKASuHFCLKQAAS 2
1 MGSLQERLARMGAPDVRFIIVNEKSPQSRALHGELELHAPPGVPVYGQPELGPDIWSILGGAKDDFLIYDR 2
1 CGRLTFHIRLPFSFLHFPYVESAIRFTHRQDSCGNCSFYPAQ 0
0 VNSTDERKGESKHSPGLEGEGQEPLGEKPDSRGLTLGSSAPTAHAHDPVHGGMEKPSPSLPSPALEPSLHEGEAPG* 0

>SEPP2_macEug Macropus eugenii (wallaby) trace archive tga stop taa
0 MGLPGLAAALLLGLVGATLASENGTRICQPAPKWQVDRGASPMEEALGQVTVVALLKASuHFCLKQAAS 2
1 IGNLQERLARVGATGVRFIIINEKSPLSQALYGELELHAPSGVLVYNQQGPGPDIWSILGGAKDDFLIYDR 2
1 CGRLTFHLPLPLSFLYFPYVESAIRFTHQQDHCGNCSFYPAQ 0
0 VNNTDKGKAESLTQSPRLEGEGQESLAEGPSTHGPTLWPSTPHPAHARGPVHSPGSKRLSPSLPPPSLEWPPHEEANK* 0

>SEPP2_ornAna Ornithorhynchus anatinus (platypus) synteny last exon uncertain
0 MAGSGLLGPALTLATLLAAAGALPDLENGTRICQPAPRWTVNGVAPMEGTEGQVIVVALLKASuHFCLKQAAR 2
1 LAGLRERLAGHGAGNVSFLIVNQRDPTAQLLHTELERHAPPGVPVYAQDGPDPDVWSVLGGDKDDFFVYDR 2
1 CGRLTFHIQLPFSFLHFPYVEAAVRFTHRRDFCGNCSYYFPQVGT 0
0 VNDTTTQESELEKSPGAPGEEPEGSPVREPDRPQSQDPTGPFSGVLLQGKENKIIPWKTPLQAAPRKPSHPPGAHD* 0

>SEPP1_tacAcu Tachyglossus aculeatus (echidna) EUEMSW408ERBZB length=227 run=R_2007_08_22_12_11_10_ 59%
1         VPVYAQDGPDPDVWSILGGGKDDFLVYDR 2
1 CGRLTFHIRLPFSFLHFPYVEAAVLFSHRHDFCGNCS

>SEPP2_galGal Gallus gallus (chicken) taa stop  synteny Zswim5 HPDL MUTYH
0 MGSLLLALASCLGLAVASEGATNGSRLCHEAPAWRINGSSPMEGAAGQVTVVALLKASuHFCLLQARS 2
1 LGALRERLGQQGVSDVRYVIVNEQAPLSRAMFGELQRHAPPGVPVLQQQPHEPDVWQLLGGDKDDFLVYDR 2
1 CGRLAFHIQLPYSFLHLPYVESAIRFTHRKDFCGNCSLYPNSTQE 0
0 ANSTMEVPATLTPLPKQEEKESETPAHHQPNHLHPHHRAVGNGTAPEPSGDHRPAHAHHHHGAHGKLHPKGQTPEGRDP* 0

>SEPP2_taeGut Taeniopygia guttata (finch)
0 MGLLVLALATWLGLGLASASEETANSSRICQEAPAWTINGSSPMEGAAGQVTVVALLKASuQFCLRQAHS 2
1 LGGLRERLARQGMADVRYMIVNEKAPLSRAMLPELQRHAPPGVPVFQPEREDPDVWQVLGGDKDDFLVYDR 2
1 CGRLAFHIQLPFSFLHFPYVEAAIRSSHIKDFCGNCSLYPNTTQE 0
0 ANSTMEGPATPSSLPEHEGMESETPVHQHKPLHPHRHHEVNSERDTNPSEDHKPATHAHHHHGDHGQLHHKGRKQKEGDEH* 0

>SEPP2_anoCar Anolis carolinensis (lizard) scaffold_4:5,993,109-5,994,846
0 MDYSLATRILLLGLVVISATQAEVTENKTRICQPAPLWKINGTAPMAGMEGQVTVVALLKASuPFCLKQAAK 2
1 IGGLQKKLSNEGVANVSFLIVNEKTPLSRAMYWELKRNAPQGIPVYQQQILEPDVWQILDGDKDDFLIYDR 2
1 CSRLTFHIQLPYSFLHLPYVEAAVHYTHRKDYCGNCSRYYSE 0
0  * 0

>SEPP2_xenTro Xenopus tropicalis (frog) NM_001006907 misannotated, no selenocysteine
0 MHNLALTVSILMGLLGQVSSSEQTNSSICKPPPKWSIEGEVPMAEALGKVTVVALLQASCGFCLVQAAR 2
1 MGPLRYKLSLQGMTDIKYMIVNDQSLHSANMFPELKRWAPEGIPVYQQTPGQDDVWELLDGNKDDFLIYDR 2
1 CGRLTFHVRLPLSFLHFPYVEAAILSTYNESFCGNCSFTSNSTLIPM 0
0 NGTTVSPSGDDSSSPLQNKDEPVNKEPSPTLEKHNDQRKLDSELRLHDHSQHHPINSHKRQENQNNHPRNLIKNGKQN* 0

>SEPP2_danRer Danio rerio (zebrafish) 3 possible tga-sel taa-stop
0 MQALWPLLLSALPALLGASSLFVEKESNGSRICKPAPQWEIDGKTPMKELLGNVVVVALLKASuHFCLTQAAR 2
1 LGDLRDKLANGGLTNISFMVVNEQDSQSRAMYWELKRRTAQDIPVYQQSPLQNDVWEILEGDKDDFLVYDR 2
1 CGYLTFHIVLPFSFLHYPYIEAAIRATYHKNMCNCS 0
0 LNANFSISESSDSTKNEPAGENNQRPNSTEPVTAAHHHHHQQHEPHHHHNPYPNNHKKSGDSDVTGKPKEPTHHSHQEHVHNHRFYTVFSFIILFYIMFFFFVNIWSCSCVRTSYLPQRG
VFGCLQCIFVLNEVYTLNLRQGSVCGCuTQESFIYTKLLISPLFGKMuLCuTKWLLGLLC* 0

>SEPP2_tetNig Tetraodon nigroviridis (pufferfish) from cDNA, genome misassembled
0 MSSPWLLWLQVALTGLLWASQGQSATSRICKAAPRWEVGGQAPMEALVGRVVVVALLKASuQFCHIQASK 2
1 IGPLREKLSRRNVTEVSFVIVNEQEPVSRALYWQLRRRAPPGVPVYQQAPLQDDVWEALDGDKDDFLIYDR 2
1 CGQLTFHIGLPYSSLRYTYVEAAVIATHQGNICNCS 0
0 ANFTSLSISNSSGSGGMPSQTNQTVTAETDGPHTTHHHPHPHRHHHHHQHLSPEQPTPTAMPGQATPTPA*

>SEPP2_gasAcu Gasterosteus aculeatus (stickleback) tga-sel taa-stop
0 MQALWPLLYAALPGLLWASHVSLLVEGDNDASRICKPAPHWTIKERAPMQELLGNVVVVALLKASuQFCLTQASK 2
1 IGGLRDKLNRSNLTDVSFMIVNEREPHSRAMYWELKRRAPPGVPVYQQAALQSDVWEALDGDKDDFLVYDR 2
1 CGLLTFHIVLPYSFLHNVYVEAAIRATYLKNICNCT 0
0 VDSVVSSLNNSVMNNETDFNVSQTNATPRIQPDTNDPEGAGTPPPPTHHHSHHHHHHHQHHHPHHPHLNQPLHDTSHPLSVHHHHHPHHHHHQQQQPGNHSGTAVDH* 0

>SEPP2_oryLat Oryzias latipes (medaka)
0 MLRLCSALPALLWASLSVLSAEGDSNASKICKPAPYWDIEGHVPMQEHLGNVVVVALLKATuEFCLTQASK 2
1 IGNLRDKLNRNNITEVSFMIVNELEALSQTMHWKLKKKAPTGVPVYQQSSLQKDVWEILDGDKDDFLIYDR 2
1 CGLLTFHIVLPNSFLQNADVENAITATYTQDICNCS 0
0 GNSTLSGGGNNFTRNSSQSHSGVQRPADEPENVTTTLEASPNDSRHEHAHHHHPHHQHLHHHHPHHQHLHHHHPHHQHLHHHHPHLQQHSKHQNDDSY* 0

>SEPP2_cypCar Cyprinus carpio (carp)
0 MQALWALWLSAFPALLWASPLFLERESNASRICKPAPKWEIDGEIPMKNLLGNVVVVALLKASuQFCLTQAAR 2
1 LGKLRDKLALGKLTDISFLVVNEQDAQSRAMYWELKRRTTEGIPVYQQSPLQNDVWDILEGDKDDFLVYDR 2
1 CGHLTFHIVLPFSFLHYPYIEAAIRATYHKNICNCS 0
0 LHANSSISEGFKNNTNNANKQTEVKNQKTNNTEPVTVEHHHHRHEHHHQHVHYHYHQHVHHHHQNKNNSTGDSDVTSVPKEPIQHSHQEHS

>SEPP2_oncMyk Oncorhynchus mykiss (trout)
0 MQGLLTLWLCAALPGLLCASPLLVEGDNDASKICKPAPHWEIKGHEAPMKELLGNVVVLALLKASuHFCRTQASK 2
1 LGGLRDKLLRSNLTDVSFLIVNEREAQSRAMYWELKRRAPPGIPVYQQAPLQDDVWEALDGDKDDFLVYDR 2
1 CGRLTFHIVLPYSFLHYPYIEAAVRATYHKDICGNCT 0
0 VDSNTTSSAGWNSTRRNETLSSSQMRVNETDSTVRSIDVDTVSNPVPSDGPQMSAEGGW* 0

>SEPP2_squAca Squalus acanthias (spiny_dogfish)
0 MGLEMPLLMILLGLTITVAVAKNETRICQVAPHWGIANQSPIEEQFGHVVVVALLKASuQFCLTQAAK 2
1 LGSLRDKLARQGLKDVRYMIVNEKTPRSRAMLWELKRHAPKDVPVYQQSPFQPDVWRILQGNKDDFLVYDR 2
1 CGKLTFHIVLPYSYLHFRYIEAAIRATYNKDICGNCS 0
0 VSDITLERERNITTTYKVTQSNTTSL

>SEPP2_leuEri Leucoraja erinacea (skate)
0 MGLQRSLLIILVGAAITLAAANNDTRICQVAPHWEIGNQSPMEQLSGQLVVVALLKASuQFCLTQAAK 2
1 LGILRDKLSLQGLKNIHYIIVNEKTLESRAMFWKLKLKTPKNITVYQQSAFQPDVWRILRGNKDDFLIYDR 2
1 CGKLTFHITSPYSYLNFRYVEAAIMATYNTDYCGNCM 0
0 GSSTTLEATSYISTGPNWEYSQHHSRSLKuRYFKIGEuWKKFPSQIRAKCQY

>SEPP2_calMil Callorhinchus milii (elephantfish)
1 LGSLQDKLARQGLKDVHYMIVNEKAPESRAMLWELKRHVPNNVSVYQQSPIQPDVWHSLQGGKDDFLIYDR 2
1 CGRLTFHVVLPYSSLQYPYIEAAIRATHKRDICGECT 0

SELU1: 13 vertebrate sequences

>SELU1_homSap Homo sapiens (human) chr10 processed pseudogenes chr8 and chr12
0 MSFLQDPSFFTMGMWSIGAGALGAAALALLLANTDVFLSKPQKAALEYLEDIDLKTLEK 1
2 EPRTFKAKELWEKNGAVIMAVRRPGcFLCREE 0
0 AADLSSLKSMLDQLGVPLYAVVKEHIRTEVKDFQPYFKGEIFLDEK 0
0 KKFYGPQRRKMMFMGFIRLGVWYNFFRAWNGGFSGNLEGEGFILGGVFVVGSGKQ 0
0 GILLEHRENEFGDKVNLLSVLEAAKMIKPQTLASEKK* 0

>SELU1_monDom Monodelphis domestica (opossum) tgt-cys
0 MSFLDLNFFSMSMWSLGAGALGAAALSLILANTDLFLTKSVDATLEFLEEIQLKTLDN 1
2 ESPKTFKARELWEHRGAVIMAVRRPGCFLCREV 0
0 AADLSALKPQLDLLGVPLYAVVKEKIGSEVENFQPYFKGKIFLDER 0
0 KKFYGPQKRKMMFMGFVRLGVWQNFFRARSKGFSGNLEGEGFVLGGVYVIGPGKQ 0
0 GILLEHREKEFGDKVNPASVLEAAKKIKPHTSTSEGK* 0

>SELU1_macEug Macropus eugenii (wallaby) EX196548 full
0 MSFLDLSFLSMGMWSLGAGALGAAVLSLILANTDVFLTKSVTATLEFLEDIELKTLDN 1
2 KTFKARELWEHRGAVIMAVRRPGCFLCREE 0
0 AADLSALKPQLDQLGIPLYAVVKEKIGSEVEDFQPYFKGKIFLDER 0
0 KKFYGPQKRKMMFMGFVRLGVWQNFFRARSKGFSGNLEGEGFILGGVYVIGPRKQ 0
0 GILLDHREKELGDKVNPASVLEACKKIKLHA* 0

>SELU1_triVul Trichosurus vulpecula (opossum) EC360881
0 MSFLDLSFFSMGMWSLGAGALGAAVLSLILANTNLFLTKSVTATLEFLEEIELKTLDN 1
2 ESPKTFKARELWEHRGAVIMAVRRPGCFLCREE 0
0 AAELSALKPQLDQLGIPLYAVVKEKIGSEVENFQPYFKGKIFLDER 0
0 KKFYGPQKRKMMFMGFVRLGVWQNFFRARSKGFSGNLEGEGFILGGVYVIGPGKQ 0
0 GILLEHREKEFGDKVDPASVLEAA   * 0

>SELU1_ornAna Ornithorhynchus anatinus (platypus) taa early stop full
0 MPLPPDLGLFNLGMWSVGVGALGAAAVGLLLANTDLLLTKPEKATLEYLEDTELKTLGK 1
2 EPRTFKARELWQRNGAVIMAVRRPGuFLCREE 0
0 AAELSSLKPQLDRLGVPLYAVVKEKIGTEVEDFQPYFKGEIFLDER 0
0 KKFYGPHKRKMLFLGFIRLGVWQNFLRARNRGFSGNLEGEGLILGGVYVLGAGKQ 0
0 GILLEHREREFGDKVSPASVLEAAQRIKPQPL* 0

>SELU1_tacAcu Tachyglossus aculeatus (echidna) 454:EUEMSW405C31QQ (74%) tSASEKK terminus? frag
0 1
2 EPRTFKARELWQRNGAVIMAVRRPGuFLCREE 0
0 AAELSSLKPQLDQLGVPLYAVVKENIGTEVEDFQPYFKGEIFLDER 0
0 KRFYGPHKRKMLFLGLIRLGVWQNFIRARNKGFPPVTWEGEG     0
0 GVLLEHREREFGDKVSPASVLEAAQKIKPQ* 0

>SELU1_galGal Gallus gallus (chicken)
0 MSFLPDFGIFTMGMWSVGLGAVGAAITGIVLANTDLFLSKPEKATLEFLEAIELKTLGS 1
2 EPRTFKASELWKKNGAVIMAVRRPGuFLCREE 0
0 ASELSSLKPQLSKLGVPLYAVVKEKIGTEVEDFQHYFQGEIFLDEK 0
0 RSFYGPRKRKMMLSGFFRXGVWQNFFRAWKNGYSGNLEGEGFTLGGVYVIGAGRQ 0
0 GVLLEHREKEFGDKVSLPSVLEAAEKIKPQAS* 0

>SELU1_taeGut Taeniopygia guttata (finch)
0 msflpdfgiFTMGMWSVGLGAIGAAVTGIVLANTDLFLSKPEKATLEFLEEIELKTLGS 1
2 EKRTFKAGELWKQNGAVIMAVRRPGuFLCREE 0
0 ASELSSLKPQLSKLGVPLYAVVKENIGTEVEDFQHYFKGEIFLDEK 0
0 KGFYGPRRRKMMLSGFFRLGVWQNFVRAWRSGYSGNLEGEGFTLGGVYVIGAGRQ 0
0 GVLLEHREKEFGDKVSLPSVLEAAEKIKPQAS* 0

>SELU1_anoCar Anolis carolinensis (lizard)
0 MWTIGLGAIGAAVTGIILANTDLFLSKAEQASLDFLEAIDLKTLGE 1
2 NQRTFKAEELWKKNGAVIMAVRRPGuFLCREV 0
0 AAELSSLKPQLDKLGVPLYAVVKENLGTEVMDFQPYFKGEIFLDEK 0.
0 KQFYGPQKRKMLFMGFIRCSVWRNFFRAWKSGYTGNIDGEGFVLGGVFVVGPGKQ 0
0 GVLLEHREKEFGDKVSLDAVLEAVKNIQPQPSEKDK* 0

>SELU1_takRub Takifugu rubripes (fugu)
0 MGLLAKLLAAVGGFVTAVMNSVTDAFLTPPLRATLEHLEETDLKTLSG 1
2 ALVIRLIPTRTETKTFKAKSLWENSGAVVMAVRRPGuFLCRE 0
0 EAAELSSLKPRLDQLGVPLYAVVKEDVGTEIQNFRPYFQGEIFLDEK 0
0 RRFYGPRERKMGLLGFLRVGVWMNGLRAFRSGFMGNVLGEGFVLGGVFVIGREQQ 0
0 GILLEHREREFGDKVNIEDVIQAVDRIAQELMPVTQN* 0

>SELU1_calMil Callorhinchus milii (elephantfish) frag
2 ENRTFRASELWAGRGAVIMAVRRPGuFLCRE 0
0 AAALSSLRPSLAQLGVPL
0 GHLLEHREKEFGDAVNLTAVMEAAGKISPRQSAE* 0	
	
>SELU1_squAca Squalus acanthias (spiny_dogfish) also selenocysteine
0 MVVVVEDFHMGLWTLGLGALGAAITGVILANTDLLLPKAETASLAYLSGAELRTLDR 1
2 EERTLKAGDLWSRSGAVIMVVRRPGuFLCREE 0
0 AAEISSLRPQLDELGVPLYGVIKENINNELKNFQPFFKGEIFLDVE 0
0 MRFYGPKPRTMGLMGFMRLGVWKNFVRAWQKGFSGNTDGEGFILgGVFVIGAGQQ 0
0 GVLLEHREKEFGDVVNISSVLEARRKIETQRTEP* 0

>SELU1_braFlo Branchiostoma floridae (amphioxus) 8.9 m traces somewhat different intronation
0 MGWRLLVA 00 TMGWVVNAVGVAVVGMVAVNLNFWLPKHIPAQLVHIA 1
2 DAALEELDGNKKKLQAQELWKNHGAVIMAVRRPGuQLCREE 0
0 ALGLSSLKPQLDRAGVPLYAVVHERKGVPEFQPYFQGKVFLDLE 0
0 RRFYGPHERWMSLAGLLRVNFWLNIRRVEKKVEGNYEGEGRLLGGVFVVGSGNQGILMQHAEQEFGHHANLTDIMKAVGEIQHINAKSKI* 0

SELU2: 1 vertebrate sequence

>SELU2_homSap Homo sapiens (human) 7 exons chr1p36.32 36% id NM_152371
0 MSTVDLARVGACILKHAVTGE 0
0 AVELRSLWREHACVVAGLRRFGCVVCRWIAQDLSSLAGLLDQHGVRLVGVGPEALGLQEFLDGDYFAG 1
2 ELYLDESKQLYKELGFKR 2
1 YNSLSILPAALGKPVRDVAAK 0
0 AKAVGIQGNLSGDLLQSGGLLVVSK 1
2 GGDKVLLHFVQKSPGDYVPKEHILQVLGISAEVCASDPPQ 0
0 CDREV* 0

>SELU2_strPur Strongylocentrotus purpuratus (sea_urchin)
0 MAFDLSKIASNLVKNFATGE 0
0 MVAISSLWEKQACVIHFMRRFGuSACRLGASELDSLRPQLDEADVRLVGIGLEDLGAQEFLDGGFWKG 1
2 DLFIDQQQKSYQGLGFKKYSLMSIMKAVFSAKTRAALSR 0
0 ASDKKIKANLKGDKLQIGGTLIISK 1
2 GGEKVLVDFKQEAPGDHIPLQTVLDAFGIKGETPKTEGAEGGTVVCNDDVCQMK* 0

SELU3: 3 eukaryote sequences

>SELU3_homSap Homo sapiens (human) 6 exons chr9q22.32 25% id processed pseudogene chrX
0 MAAPAPVTRQVSGAAALVPAPSGPDSGQPLAAAVAELPVLDARGQRVPFGALFRERRAVVVFVR 0
0 HFLCYICKEYVEDLAKIPRSFLQ 0
0 EANVTLIVIGQSSYHHIE 0
0 PFCKLTGYSHEIYVDPEREIYKRLGMKRGEEIASS 1
2 GQSPHIKSNLLSGSLQSLWRAVTGPLFDFQGDPAQQGGTLILGP 1
2 GNNIHFIHRDRNRLDHKPINSVLQLVGVQHVNFTNRPSVIHV* 0

>SELU3_petMar Petromyzon marinus (lamprey) cDNA EE741479 ti|1430987375 tga-sel taa-stop frag
             VRPDPYIYKALELKMGEDVDKIY 1
2 KSPHVHCGLWWGVARGLWRAMWSESFDFQGDPKQQGGALVLGP 1
2 GGRVLFSHRDEAVLDHTPINRLLAVAGIPPVDFTHKHMVKHVuPRPLTPTG* 0

>SELU3_monBre Monosiga brevicollis (choanoflagellate) tga-sel
MATGAGVGASWEDVAKMTVYDHQQEPCSLGSLCSPGQMTAIGTLARDNSARVPPPLITHHATLPPRVCRLVFVR
HFLuFVCKDYVTDLARVPDEHWA
GARVVVIGCANPVFID
RFAADTGFPASNVYCDPKREVYQALQLPSVLAAINA
SKSIHIHSSALGGTMNSIYRGLRAMRAQGNVKQQGGAFVVNG
QGQLIYAHRDQRPEDHCPINTLLEQVGGRPFF*

SELV: 19 placental mammal sequences

>SELV_homSap Homo sapiens (human) chr 19 uc002oly.1  66 pro + 32 thr +21 ser first exon
0 MNNQARTPAPSSARTSTSVRASTPTRTPTPLRTPTPVRTRTPIRTLTPVLTPSPAGTSPLVLTPAPAQIPTLVPTPALARIPRLVPPPAPAWIPTPVPT
PVPVRNPTPVPTPARTLTPPVRVPAPAPAQLLAGIRAALPVLDSYLAPALPLDPPPEPAPELPLLPEEDPEPAPSLKLIPSVSSEAGPAPGPLPTRTPL
AANSPGPTLDFTFRADPSAIGLADPPIPSPVPSPILGTIPSAISLQNCTETFPSSSENFALDKRVLIRVTYC 2
1 GLuSYSLR 0
0 YILLKKSLEQQFPNHLLF 0
0 EEDRAAQATGEFEVFVNGRLVHSKK 0
0 RGDGFVNESRLQKIVSVIDEEIKKR* 0

>SELV_panTro Pan troglodytes (chimp) essentially identical to humna
MNNQARTPAPSSARTSTSVRASTPTRTPTPLRTPTPVRTRTPIRTLTPVLTPSPAGTPPLVLTPAPAQIPTLVPTPALARIPRLVPPPAPAWIPTPVPTPV
PVRNPTPVPTPARTLTPPVRVPAPAPAQLLAGIRAALPVLDSYLAPALPLDPPPEPAPELPLLPEEDPEPAPSLKLIPSVSSEAGPAPGPLPTRTPLAANS
PGPTLDFTFRADPSATGLADPPIPSPVPSPILGTIPSAISLQNSTETFPSSSENFALDKRVLIRVTYC 2
1 GLuSYSLR 0
0 YILLKKSLEQQFPNHLLF 0
0 EEDRAAQATGEFEVFVNGRLVHSKK 0
0 RGDGFVNESRLQKIVSVIDEEIKKR* 0

>SELV_ponPyg Pongo abelii (orang_borneo)
0 MNNQARTPAPSSARTSTSVRASTPTRTPTPLRTPTPVRTRTPIRTLTPVLTPSPAGTPPLVLTPAPAQIPTLVPTPALARIPRLVPPPAPAWIPTPVPTPVPVRNPTPVPTPARTLTTPV
RVPAPAPAPPPAQVLAGIRAALPILDSYLAPALRLHPPPEPAPELPLSPEEDPEPAPSLKLIPSVSSEAGPAPGPLPARTPQAANSPGPTLDFTFRADLSATGLADPPIPSPVPSPILGT
TSSAISLQNSTENFASSSENFALDKRVLIRVTYc 2
1 GLuSYSLR 0
0 YILLKKSLEQQFPNHLLF 0
0 EEDRAAQATGEFEVFVNGRLVHSKK 0
0 RGDGFVNESTLQKIVSVIDEEIKKR* 0

>SELV_macMul Macaca mulatta (rhesus) distal seq from M. nemestrina transcript
0 MNNQARTPAPSSARTSTSVRASTPTRTPTPLRTPTPVRTRTPIRTQTPVLTPSPARLPALVQPPAPAHIPTQVPTPALARIPGLVPPPARAWIPTPVPTPARVRNPTPVPTPARTLTPPV
RVPAPAPDPAQVLAGIRTALPALDSYPAPALSLDSPPEPAQEPPLSPEEDPEPAPSLKLIPSVSSEAGPALGPLPAHTPLAAKSSGPTLDFTFRADPSATGLADPHIPSPVPAPILGTIP
SALSLQNFTETLVSTSENFALDKRVLIRVTYC 2
1 GLuSYSLR 0
0 YILLKNSLEQQFPSHLLF 0
0 EEDRAAQATGEFEVFVNGRLVHSKK 0

>SELV_calJac Callithrix jacchus (marmoset)
0 MNNQARTPAPSSARTSTSVRASTPTRTPTPVRTPTAVRIRTPIRTLTPSLAGTPALVPTPTPARISRLVPTPAPARTPTPIPTLVRTLTPVPLPAPARIPAPAPAPAPAPAPAPSPALVP
AGIRATLPVLDSYPALALPWDPPPEPVPEPLVSVSSEEDPEPAPSLKLVPSVSGETGPAPGPLPACTPLATNPPEPTLDFTADSSATELAVPTIPGSVPAPILGTIPLAASLLNSTESFL
SASENFALDKRVPIRVTYc 2
1 GLuSYSLR 0
0 YILLKKSLEQRFPDCLLF 0
0 EEDRAAQATGDFEVFVDGRLVHSKK 0
0 RGDGFVDEASLQKIVSVIDEEIKKR* 0

>SELV_otoGar Otolemur garnettii (bushbaby)
0 MNSQARASVHPSRTSTAVRASIPARVHPRARTTPVQPRTLITQDRIPAPVRVPP
1 GLuSYGLQ 0
0 YILLRQNLEHHFPNRLLF 0
0 EEGRAAQATGEFEVFVDGKLVHSKK 0
0 NGDGFVDEIRLQKIVNIIDEEIKKRQ 0

>SELV_musMus Mus musculus (mouse) AV279316 58 Pro, 25 Thr, 25 Ser syntenic chr 7 46% hsa!
MNNKARVPAPSSVRANTPARTPAPIRTATPVRAPNPAHNSTPVRTSIRVRAPAQVPNPVPIRFPTPAPVPAPTLTPAPTPAPVRHAAPVRTPAPVRAPNLGRV
FPKISPGRFFPSLASPTAQPLSSRAASALLKDPTLAQNQKPSIHSLAEAIQGPLPVLTPSSSKTQGSIPDTASPIDSLASTAMASSTLGPIPGPNPTLEFLAS
PLKETPGLGKLSTISPAPSFGSTKEIPSTSEDVPTPNRILIRVMYC 2
1 GLuSYGLR 0
0 YIILKRTLEHQFPNLLEF 0
0 EEERATQVTGEFEVFVDGKLIHSKK 0
0 KGDGFVDESGLKKLVGAIDEEIKKR* 0

>SELV_ratNor Rattus norvegicus (rat) chr 1 synteny 83% mmu
MNNKARNPAPSSVRANTPSRTPTPVRTATPVRASTPAHNRSPVRTSIRVRTPANPVPIRFPTPAPAPAPTPTPAPTPAPVPAAAPVRTPAPVRASIQGRSFPTIS
PVRFLRNLALPAAQPLSSGGAGSLSKDLTLAQKQKPSIHSLAEAIQGPFPVLTPSASSETHGSIPDPAPPTDSLASTAMASSTLDPIPGPKPTLEFLASPLKETP
DLGKLSTISPAQNFVSTKEVPSTSEDVPTANRILIRVMYC 2
1 GLuSYGLR 0
0 YILLKKTLEHQFPNLLEF 0
0 EEERATQVTGEFEVFVDGKLIHSKK 0
0 KGDGFVDETSLKKLVGAIDEEIKKR* 0

>SELV_cavPor Cavia porcellus (guinea_pig)
1 GLuSYGFQ 0
0 YTLLKMSLKQQFPNLLRF 0
0 EDERATQVTGEFEVFVEGKLVHSKK 0
0 QGDGFVDDNRMQTIVNAINEEIKRR* 0

>SELV_oryCun Oryctolagus cuniculus (rabbit)
0 MNNQARTPAPAQARTSSVVRASAPTRVSTQIRTPATGWTPTPVQASTPVRTQTPVRTPTLVQASTPVRTPTLGQASTPVRTPTPVQALTPVRTPTPVSGPDSGRTPTPVGTPVGTL
1 GLuSYGLR 0
0 YILLKKNLEELFPDCLLF 0
0 EEERATQASGEFEVFVNGKLVHSKK 0
0 KGDGFVDEVKLRKIVTAINEEIK... 0

>SELV_canFam Canis familiaris (dog) CO599097
0 MNNQARAPPRTSARVLAWVRASTPVRTSIPVRTPTPARIPTSSRAPTSVQTPAPARTPNPVQIPTPVQTSTSARIPNPARTLTPVQTPASAWTPNPVQMLTPARTPTPVPTP
VPTPIPARTPTPARTPTPVEAAAAAPASESFGSSALPLEPPPEPASEPTTSPHQDLSPTPSVKPLPSVTNGFGSTQEPLPDLTPPATDFLGPTLGSTSRADSSATKLTDSSESVR
VPIPGTPSATALATSTNTFAPVGESCSVKIAVRVIYC
1 GLuSYGLR 0
0 YILLKKSLEQQFPNCLLF 0
0 EEERAAQATGEFEVFVDGKLVHSKK 0
0 KGDGFVDEARLQKIMNVIEEEVRKR* 0

>SELV_felCat Felis catus (cat)
0 MNNQARAPNPSPARVLALVRASTPVRSSIPVRIPTPARIPARTRNPQTPVRAPNPVQIPNPVQISARASNPARAPRPVQIPARTPNPPQTLTPGRAPNPVPTPVQTPTLVQTP
TPVQTPTPVWTETPVQTPTAVGTPTRVQTLTPVIPRVRTPTPIRPLWPDPSPNLIPSGPDHPAPESSLRNSAPSFWINSPDSLPAPILETPSTAFASTFENLPEDSKILIRVIYC
1 GLuSYSLR 0
0 YILLRKSLELQFPNCLRF 0
0 EEERSAQATGEFEVFVDGKLVHSKK 0
0 KGDGFVDEARLQKIVSTIDEEIRKR* 0

>SELV_bosTau Bos taurus (cow) DT829759
0 MNNQPRTPAPTPARASTPVRGSTLHRTSIQVRTPTPGPDSGPTRITTLLRTPALIRTLTPIRTPTPVRTPTPVPPSTPVRSPIPVRTPTPVPPSTPVRTPTPVPLSTPVRPPTPVRPPTPVRPPTPVRPPTP
IGTPTLIRSPTPVQIPIPEPIPTPIPSRVLIPPLESFPDSALPSGPPLELEPTLTVSPAKNLEPSPAKNLEPSPRVKQVSSAANGFPPIQEPLPALTPLATDLRSPSLGSPLRTDTSTTNLIASSSGHVPGTPILGAIQAILPVPATALASISGNLKEENKIMIRVVYC
1 GLuSYSLR 0
0 YILLRKSLEQQFPNSLIF 0
0 EEEISAQATGEFEVFVDGKLIHSKK 0
0 NGDGFVDEVKLQTIVNLLNEEFKKR* 0

>SELV_susScr Sus scrofa (pig) ti|851198642 CX061656
0 MNNPARAPAPSPVRPSASLRPLASVRASTPARGSTLARTSLLVRTPRTPNLVPASGPGPIRTPTPVRTPTPVRTPTPVRTLTPVRTPTPVRTPTPVRTPTPVRTPTPVRTPTPVQVPTPVRVPTTVGIPTP
TLSQVLVPALESLPNPALPSLDPPLEPDPELTLSPDEDPAPTPRAKHLPLVANGFVPVQEPLPALSPLATNLLESTPGAGTDSSTTKLTDSTSGHVPGTPILATIPLAVALPVSTNALASTSENIQVEQQILIRVVYC 2
1 GL*SYGLR 0
0 YILLKKSLEQQFPNCLVF 0
0 EEDISAQATGAFEVFVDGKLVHSKK 0
0 KGDGFVDETKLQKIVSHINEEIKTKVAGSC* 0

>SELV_equCab Equus caballus (horse)
1 GLuSYGLR 0
0 YILLRKSLDQQFPNRLVF 0
0 EEDVGAQASGEFEVFVEGKLVHSKK 0
0 KGDGFVDEARLRKIVSAINEEIKRR* 0

>SELV_myoLuc Myotis lucifugus (microbat)
0 MNHQARASHPFPARTPASVRASIPVRPSNQTPAPSSAPTRTSIPVPASTAVRTPTLVRTPIPFQAVSPVRTSTRFSASVPVQFPTPVSASTPVQTPTRVPAPVRTSTRFPASV
PVQFPTPVPASTPVRTSTPVPASTPVQTPTRVPAPAQVRTSIPVPAPAPARTSTPVPAPAPVRTSTTVPAPVPFRTPTPVPAPAPVRTSTPVPAPAPVRTSTPVPAPGPNPDSSPCPGPG
1 GLuSYNLR  0
0 YILLKKSLEQQFPNCLYF 0
0 EEDISEQATGEFEVFVDGKLVHSKK 0
0 KGDGFVDEVKMQKIVNIIDEEIRKN* 0

>SELV_pteVam Pteropus vampyrus (macrobat)
0 MNNKARASAPSLPGTSALVRASAPVRPSTPVRAPTLIRTPTPVRTPNPVPASTPARVPTPVRTLTPVRTPTPIRTPTPVRTQIPFRAANPVRAANPVRTPTPLRTPTPVRTSIPVRTL
APVSTPTPIPPPTPPAVPAVVPAAVPGLEFFPSPPPEPVPEPALSPDKDPAPTQSVKHVPSVANEFGLTQEPLSALAPLATDLLGPTRASIRRADPEATKLTGSTAEPSPASILELISSAHIGIASEYLQVDTGSVIRVILL
1 GLuSYNFR 0
0 YILLRKNLEQQFPHGLLF 0
0 EEEVSAEGTGEFEVLVDGKLIHSKK 0
0 RGDGFVDEARLQKIVNVINE IRKR* 0

>SELV_dasNov Dasypus novemcinctus (armadillo) ti|593909023
1 GLuSYALR  0
0 YILLKKSLEMQFPNRLIF 0
0 EEARSSQAVGEFEVFVDGALVHSKK 0
0 RGDGFVDDSKMEKIVSSIEEAVKKT* 0

>SELV_loxAfr Loxodonta africana (elephant)
0  2
1 GLuSYGLR 0
0 YILLRKSLEQQFPNHLIF 0
0 EEDISSQATGEFEVFVDGKLVHSKK 0
0 KGDGFVDDTRLQKIVNSINETIKKE* 0

>SELV_proCap Procavia capensis (hyrax)
0  2
1 GLuSYSLR 0
0 YILLRKNLEQQFPNHLLF 0
0 EVDISSQATGEFEVFVDGKLVHSKK 0
0 KGDGFVDDAQLQKIVNSINETIQRR* 0

SEPW: 27 vertebrate sequences

>SEPW_homSap  Homo sapiens (human) Selenoprotein W  chr19 87 aa uc002phn.1 has retroprocessed pseudogene
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 CGEGTPQATGFFEVMVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_panTro Pan troglodytes (chimp)
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 RGEGTPQATGFFEVMVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_ponPyg Pongo pygmaeus (orang_sumatran) CR926472
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 CGEGTPQATGFFEVMVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_macMul Macaca mulatta (rhesus)
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 CGEGTPQATGFFEVMVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_macFas Macaca fascicularis (cynomolgus_monkey) AB169486
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 CGEGTPQATGFFEVMVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_papAnu Papio anubis (baboon) EY285690
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 CGEGTPQATGFFEVMVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_calJac Callithrix jacchus (marmoset)
0 MALTVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 SGEGTPQATGFFEVTVAGKLIHSKK 0
0 KGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_micMur Microcebus murinus (mouse_lemur)
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGCLDI 0
0 CGEGTPQATGFFEVMVAGKLVHSKK 0
0 GDGYVDTESKFLKLV

>SEPW_musMus Mus musculus (mouse)
0 MALAVRVVYC 2
1 GAuGYKPK 0
0 YLQLKEKLEHEFPGCLDI 0
0 CGEGTPQVTGFFEVTVAGKLVHSKK 0
0 RGDGYVDTESKFRKLVTAIKAALAQCQ* 0

>SEPW_ratNor Rattus norvegicus (rat) BC087625
0 MALAVRVVYC 2
1 GAuGYKPK 0
0 YLQLKEKLEHEFPGCLDI 0
0 CGEGTPQVTGFFEVTVAGKLVHSKK 0
0 RGDGYVDTESKFRKLVTAIKAALAQCQ* 0

>SEPW_cavPor Cavia porcellus (guinea_pig)
0 MALAVRVVYC 2
1 GAuGYKPK 0
0 YLQLKEKLEDEFPGCLDI 0
0 CGEGTPQTTGFFEVTVAGKLVHSKK 0
0 GGDGFVDTEGKFRKLVAAIKAALAQG* 0

>SEPW_oryCun Oryctolagus cuniculus (rabbit)
0 MALAVRVVYC 2
1 GAuGYKPK 0
0 YLQLKKKLEDEFPGCLDI 0
0 CGEGTPQVTGFFEVTVAGKLVHSKK 0
0 RGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_ochPri Ochotona princeps (pika)
0 MALSVRVVYW 2
1 GAuGYKPK 0
0 YLQLKKRLEDEFPGCLDI 0
0 GEGTPQVTGFFEVMVAGKLVHSKK 0
0 SGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_canFam Canis familiaris (dog)
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGCLDI 0
0 RGEGTPQATGFFEVTVAGKLVHSKK 0
0 RGDGYVDTESKFLRLVAAIKTALAQG* 0

>SEPW_felCat Felis catus (cat)
0 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGCLDI 0
0 RGEGTPQATGFFEVMVGGKLVHSKK 0
0 RGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_bosTau Bos taurus (cow)
0 MAVVVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPSRLDI 0
0 RGEGTPQVTGFFEVFVAGKLVHSKK 0
0 GGDGYVDTESKFLKLVAAIKAALAQA* 0

>SEPW_oviAri Ovis aries (sheep)
0 MAVVVRVVYC 2
1 GAuGYKPK 0
0 YLQLKKKLEDEFPSRLDI 0
0 CGEGTPQVTGFFEVFVAGKLVHSKK 0
0 GGDGYVDTESKFLKLVAAIKAALAQA* 0

>SEPW_susScr Sus scrofa (pig) AF380118
0 MGVAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGRLDI 0
0 CGEGTPQVTGFFEVLVAGKLVHSKK 0
0 GGDGYVDTESKFLKLVAAIKAALAQG* 0

>SEPW_eriEur Erinaceus europaeus (hedgehog)
0 MALAVRVVYC 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGCLDI 0
0 RGEGTPQGTGFFEVLVAGKLVHSKK 0
0 KGDGYVDTETKFLKLVTAIKAALAQG* 0

>SEPW_sorAra Sorex araneus (shrew)
0 2
1 GAuGYKSK 0
0 YLQLKKKLEDEFPGCVDV 0
0 CGEGTPQVTGFFEVMVAGKLVHSKK 0
0 RGDGYVDSESKYVRLVTAIKTALAQA* 0

>SEPW_choHof Choloepus hoffmanni (sloth)
0 MALAVRVVYW 2
1 GAuGYKPK 0
0 YVQLKKKLEDEFPGCLDI 0
0 SGEGTPQTTGFFEVMVAGKLVHSKK 0
0 QKGDGFVDTESKFLRLVAAIKAALAQG* 0

>SEPW_monDom Monodelphis domestica (opossum) diverged
0 MAIQVRVVYW 2
1 GAuGYKPK 0
0 YLLLKKKLEDEYPGLLRH 0
0 NGEGTPEVTGFFEVTVAGKLVHSKK 0
0 AGHGFVDTADKYLQIVAEIKAALA* 0

>SEPW_ornAna Ornithorhynchus anatinus (platypus)
0 MASLEAFPRGVVPVHVVYC 2
1 GAuGYKPK 0
0 FLQLKKKLENEFPGQVEI 0
0 SGEGTPQVTGWFEVTVAGKLVHSKK 0
0 EGDGFVDSESKFAKIRMAIKAALVPGY* 0

>SEPW_galGal Gallus gallus (chicken) tga confirmed
0 MPLRVTVLYC 2
1 GAuGYKPK 0
0 YERLRAELEKRFPGALEM 0
0 RGQGTQEVTGWFEVTVGSRLVHSKK 0
0 NGDGFVDTNAKLQRIVAAIQAALP* 0

>SEPW_anoCar Anolis carolinensis (lizard)
0 2
1 GAuGYSPK 0
0 YQQLKRGLEKEFPGKLEI 0
0 TGEGTPQVTGWFEVTVAGKLVHSKK 0
0 NGDGFVDNDTKLHKILMAIKAALA* 0

>SEPW_xenTro Xenopus tropicalis (frog) tga confirmed
0 MPDTMVKVNVVYC 2
1 GAuGYLSK 0
0 FRRLKKELEQRFPGKLSI 0
0 DGEGTERMTGWFEVSINGKLVHSKK 0
0 NGDGYVDNDAKLQKIILAIEAALKQ* 0

>SEPW_danRer Danio rerio (zebrafish) tga confirmed
0 MTVKVHVVYC 2
1 GGuGYRPK 0
0 FIKLKTLLEDEFPNELEI 0
0 TGEGTPSTTGWLEVEVNGKLVHSKK 0
0 NGDGFVDSDSKMQKIVTAIEQAMGK* 0

>SEPW_takRub Takifugu rubripes (fugu)
0 MGVTIRVEYC 2
1 GGuGYGPR 0
0 YEELARVVRAEFPDADVSGFVGRM 1
2 GSFEIQINEQLIFSKLETGGFPYEDD 0

>SEPW_calMil Callorhinchus milii (elephantfish)
1 GAuGYEPRYQKLAIVIKDEFPDADVSGKVGRT 1
2 GSFEIEINGQLIFSKLETGGFPYEND 0
0 ISEAVQKANNGEELQKIENSRPPCVIL* 0

>SEPW_petMar Petromyzon marinus (lamprey) cDNA FD700531 tga-sel tag-stop full
0 MPLKIHIVYC 2
1 GAuGYRSR 0
0 FHRLKDELETEFPGELEI 0
0 TGEGTPTQTGFLEVQIVGGKLLHSKA 0
0 NGDGFVDSDEKLQKIFSGVEKALKK* 0

>SEPW_eptBur Eptatretus burgeri (hagfish) cdna BJ662449 tgn-sel tag-stop
0 MPLKIHVVYC 2
1 GSuGYASK 0
0 FRALKVKLDHEFPGKLEI 0
0 TSEGTPGLTGKFEVQVGEKLVHSKK 0
0 NGDGFVDSPEKLQKIFKAVENALKGQ* 0

DIO1: 50 vertebrate iodothyronine deiodinases

>DIO1_homSap Homo sapiens (human) iodothyronine deiodinase type I 4 exons chr1 uc001cwb.1
0 MGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQ 1
2 GNRPLVLNFGSCTuPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHAS 1
2 DGWAFKNNMDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYIIQEGRILYK 0
0 GKSGPWNYNPEEVRAVLEKLHS* 0

>DIO1_panTro Pan troglodytes (chimp)
0 MGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVHLSGQRCNIWEFMQ 1
2 GNRPLVLNFGSCTuPSFMFKFDQFKRLIEDFSSIADFLVVYIEEAHAS 1
2 DGWAFKNNMDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRILYK 0
0 GKSGPWNYNPEEVRAVLEKLHS* 0

>DIO1_ponPyg Pongo pygmaeus (orang_sumatran)
0 MGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTETGGLAPNCPVVRLSGQRCNIWEFMQ 1

>DIO1_macMul Macaca mulatta (rhesus)
0 MGLPQSGLWVKKLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWDFMQ 1
2 GNRPLVLNFGSCTuPSFMFKFDQFKRLIEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNMDIRNHRNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRILYK 0
0 GKSGPWNYNPEEVRAVLEKLYS* 0

>DIO1_macFas Macaca fascicularis (crab_macaque)
0 MGLPQSGLWVKKLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWDFMQ 1

>DIO1_macNem Macaca nemestrina (pigtailed_macaque)
0 MVLPQSGLWVKKLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGPSCNIWDFMQ 1

>DIO1_calJac Callithrix jacchus (marmoset)
0 MGLPGPGLWLKRLWVLLEVAVHVAVGKVLLTLFPDRVKKNILAMGDKTGMTRNPNFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVHLSGQRCNIWDFMQ 1
2 GNRPLVLNFGSCTuPSFMFKFDQFKRLTEDFSSVADFLIIYIEEAHAS 1
2 DGWAFKNNVDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSELYAALPERLYIIQEGRILYK 0
0 GKSGPWNYNPEEVRDVLEKLHS* 0

>DIO1_otoGar Otolemur garnettii (bushbaby)
0 MGLPRPGLWLKRLWVFLEVAVHVAVGKMLLILFPDRVKSQILAMGQQTVMAKNPHFSHDNWIPTFLQHPVFLVCPEGPLQRLEDMTERGGLAPNCPVSASSGQKCNIWDFMQ 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLVEDFSSVADFLIIYIEEAHAS 1
2 DGWAFKNNVDIRTHRNLQDRLRAAHLLLARSPQCPVVVDTMENQSSQLYAALPERLYVLQEGRILYK 0
0 GKSGPWNYQPEEVRAVLEKFDN* 0

>DIO1_micMur Microcebus murinus (mouse_lemur)
0 MGLPCPGLWLKRLWVLLQVAVHVAVGKVLLTLFPERVMQHILSIGQKTGMARNPHFTPDNWVPTFFSTQYFWFVLKVRWQQLEDVTERGGLAPNCPVVRLSGQRCNIWDFMQ 1

>DIO1_tupBel Tupaia belangeri (tree_shrew)
0       RAGLWLKRLWVFLQLTVQVAVGKVLLTLFPERVKQHILALGQKTGIARNPNFAHDNWIPTFFSTQYFWFVLKVYWQRLEDTTEPGGLAPNGPVVHLSGQRCDIWDFMQ 1
2 GDRPLVLNFGSCTuPSFLFKFDQFKRLIEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVNIRNHQNLQDRLQAAHLLLDRSPQCPVVVDTMQNQSSQLYAALPERLYVLQEGRILYK 0
0 GKPGPWNYDPEEVRAVLEKLRS* 0

>DIO1_musMus Mus musculus (mouse)
0 MGLPQLWLWLKRLVIFLQVALEVAVGKVLMTLFPGRVKQSILAMGQKTGMARNPRFAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEFGGLAPNCTVVCLSGQKCNIWDFIQ 1
2 GSRPLVLNFGSCTuPSFLLKFDQFKRLVDDFASTADFLIIYIEEAHAT 1
2 DGWAFKNNVDIRQHRSLQERVRAARMLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYK 0
0 GKAGPWNYNPEEVRAVLEKLCTPPRHVPQL* 0

>DIO1_ratNor Rattus norvegicus (rat)
0 MGLSQLWLWLKRLVIFLQVALEVATGKVLMTLFPERVKQNILAMGQKTGMTRNPRFAPDNWVPTFFSIQYFWFVLKVRWQRLEDRAEYGGLAPNCTVVRLSGQKCNVWDFIQ 1
2 GSRPLVLNFGSCTuPSFLLKFDQFKRLVDDFASTADFLIIYIEEAHAT 1
2 DGWAFKNNVDIRQHRSLQDRLRAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYK 1
0 GKPGPWNYNPEEVRAVLEKLCIPPGHMPQF* 0

>DIO1_speTri Spermophilus tridecemlineatus (squirrel)
0 MGLLRPGLWLKKLWILLLVIVEVAMGKVLMTLFPERATQNILAMGQKTGMTRNPQFSPDNWVPTFFSIQYFWFVLKVRWQRLEDKAELGGLAPNCPVIRLSGEKCNIWDFIQ 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLIEDFSPIADFLIIYIEEAHAS 1
2 1
0 * 0

>DIO1_dipOrd Dipodomys ordii (kangaroo_rat)
0 MELSRLGLWLKRLWIFLQVVVEVAMGKMLMILFPERVKKHILAMGQKTGMTRNPRFSPDNWVPTFFSTQYFWFVLKVRWQRLEDKAMYGGLAPNCSVISLSGQRCSIWDFMQ 1
2 GNRPLVLNFGSCTuTSFLFKFDQFKRLVEDFDSTADFLIIYIEEAHAS 1
2 DGWAFKNNVNISHHRNLQDRLQAAQLLLDQKPQCPVVVDTMENQSSQLYAALPERLYVLQEGRILYK 1
0 GKPGPWNYNPEEVRAVLEKLCT* 0

>DIO1_ochPri Ochotona princeps (pika)
0 MAWPQVRLWLRRLWVLVQVAVEVAVGKVLMTLFPERVKQSILAMGQKTGLAQNPLFTHDNWIPTFFSIQYFWFILKVRWQRLEDATQPGGLAPNCPVVCLSGQECHIWDFMQ 1
2 GNRPLVLNFGSCTuPSFLSKFNQFKRLIEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVDIRNHRNLQERLQAAHLLLPRSPQCPVVVDTMQNQSSQHYAALPERLYVLQQGRILYK 1
0 GQPGPWNYDPEEVRAVLLELHS* 0

>DIO1_cavPor Cavia porcellus (guinea_pig)
0 MGLTWPGLWLKRLWVLVQVAVEVAMGKVLMTLFPERIKKSILAMGEKTGMTRNPQFSHDNWIPTFFSTQYFWFILKVRWQRLEETAELGGLAPDCSVVCLSGEKRTIWDFMH 1
2 GNRPLVLNFGSCTuPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHAS 1
2 DGWAFKNNVDIRQHQNLQDRMRAAHLLLAKSPQCPVVVDTMQNESSQLYAALPERLYVQEGRILYK 1
2 GKSGPWNYNPEEVRGVLEKLHT* 0

>DIO1_oryCun Oryctolagus cuniculus (rabbit)
0 MGLPRPGLWLKRLWVLVQVAVEVAVGKVLMTLFPERVKQNILAMGQKTGIAQNPNFAQDSWIPTFFSTQYFWFVLKVRWQRLEDATEPGGLAPNCSVVRLSGQQCSIWDFMR 1
2 GNRPLVLNFGSCTuPSFLSKFDQFKRLIQDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVDIKNHRNLQDRLRAASLLLARSPQCPVVVDTMQNQSSQLYAALPERLYVLRQGRILYK 0
0 GESGPWNYNPEEVRAVLEELHS* 0

>DIO1_canFam Canis familiaris (dog)
0 MGLPRPVLWLRRLWVLLQVAVQVAVGKVFLKLFPARVKQHIVAMNGNKPHFSYDNWAPTLYSMQYFWFVLKVQWQRLEDRTEPGGLAPNCPVVRLSGQRCNIWDFMQ 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLIEDFCSTADFLIIYIEEAHAS 1
2 DGWAFKNNVNIRTHQTLQDRLQAARLLLDRAPPCPVVVDTMRNQSSQFYAALPERLFVLQEGRILYK 0
0 GKPGPWNYHPEEVRAVLEKLHS* 0

>DIO1_ursArc Ursus arctos (bear) AM748338
0                   VAMHVAVGKVLLILFPERVKQQVLAMNKKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVRLS

>DIO1_melUrs Melursus ursinus (bear) AM748341
                     AMHVAVGKVLLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVRLSG

>DIO1_ursAme Ursus americanus (bear) AM748339
                     AMHVAVGKVLLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVRLSG

>DIO1_ursMar Ursus maritimus (bear) AM748337
                     AMHVAVGKVLLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVRLSG

>DIO1_ailMel Ailuropoda melanoleuca (bear) AM748344
                     AMHVAVGKVFLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYGMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVQLSG

>DIO1_treOrn Tremarctos ornatus (bear) AM748343
                     AMHVAVGKVLLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVQLSG

>DIO1_helMal Helarctos malayanus (bear) AM748340
                            GKVLLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAPNCP

>DIO1_ursthi Ursus thibetanus (bear) AM748342
                      AMHVAVGKVLLILFPERVKQQVLAMNQKNPHFSYDNWLPTFYSMQYFWFVLKVRWQRLEDRTEPGGLAP

>DIO1_felCat Felis catus (cat)
0 MGLSQLGLWLRRLWVLFQVALQVAVGKVFLILFPSRVKQHIVAMNRKNPHFSYDNWAPTLYSVQYFWFVLKVRWQRLEDRTEPGGLAPNCPVVRLSGQRCSIWDFMK 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLIEDFCSIADFLIIYIEEAHAS 1
2 DGWAFKNNVNIRNHRNLQDRLQAACLLLDRSPRCPVVVDTMKNQSSRLYAALPERLYVLQAGRILYK 0
0 GKPGPWNYHPEEVRAVLEKLHS* 0

>DIO1_equCab Equus caballus (horse)
0 MGLPRAGLWLKRLWVLLQVALQVAVGKVLLTLFPDRVKQHIVAMNQKNPHFSYDNWVPTLYSTQYFWFVLKVHWQRLEDTTKRGGLAPNSPVVRLSGQRCNIWDFMQ 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLIEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVVIRNHRNLQDRLRAARLLLDRSPPCPVVVDSMENRSSQLYAALPDRLYVLQARRILYK 0
0 GKPGPWNYHPEEVRAVLEKLHS* 0

>DIO1_myoLuc Myotis lucifugus (microbat)
0 MGLPQPGLWLKRLWILLQVALHVTLGKVQLKLFPRRVKQHILAMNRKNPHFSYDNWAPTLFSTPYFWFILKVRWQRLEDKTEEGSLAPNCPVVRLSGQRCHIWDFMQ 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLIEDFSAIADFLVIYIEEAHAS 1
2 DGWAFKNNVVIKNHRNLQDRLQAAHLLLDRSPRCPVVVDTMKNQSSQLYAALPDRLYVLQEGRILYK 0
0 GKPGPWNYHPEEVRAVLEKLRS* 0

>DIO1_pteVam Pteropus vampyrus (macrobat)
0 MELPWPGRWLKRLWVLLQVALHVAVGKVQLTLFPRRVKQNIVAMNRKNPHFSFDNWLPTLFSTQYFWFVLKVRWQQLEDTTKEGGLAPNCPVVCLSGQRCNIWDFMQ 1
2 GKRPLVLNFGSCTuPSFLLKFDQFKKLIEDFSSIADFLVIYIEEAHAS 1
2              ERLQAARMLLDRSPPVPVVVDTMKNQSSHLYAALHERLYVLQEGRILYK 0
0 GKPGPWNYHPEEVHAVLEKLHS* 0

>DIO1_bosTau Bos taurus (cow)
0 MGLPSPGLWLKRLWVLFQVALHVAIGKVLLTLFPRRVKQNILAMGEKTGMTRNPHFSHENWIPTFFSTQYFWFILKVRWQRLEDMTEQGGLAPNCPVVRLSGERCSIWDFMQ 1
2 GNRPLVLNFGSCTuPSFIFKFDQFKRLIEDFGSVADFLIIYIEEAHAS 1
2 DGWAFKNNVDIKNHRNLQDRLRAAHLLLDRSPPCPVVVDTMTNQSSSCYAALPERLYVLQEGRVLYK 0
0 GKPGPWNYHPEEVRAVLEKLHS* 0

>DIO1_turTru Tursiops truncatus (dolphin)
0 MGLPLPGLWLKRLWVLFQVGLHVAMGKVLLTLFPRRVKQNILAMSEKTGMAKNPHFSYENWIPTFFSAQYFWFILKVRWQRLEDMTEQGGRAPNCPVVRLSGQRCNIWDFMQG 1
2 GNRPLVLNFGSCTuPSFIFKFDQFKRLIEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVDIKNHQHLQDRLRAARLLLDRSPQCPVVVDTMKNQSSQLYAALPERLYVLQDGRILYK 0
0 GKPGPWNYRPEEVRAVLEKLHS* 0

>DIO1_susScr Sus scrofa (pig)
0 MELPLPGLWLKRLWVLFQVALHVAMGKVLMTLFPGRVKQDILAMSQKTGMAKNPHFSHENWIPTFFSAQYFWFVLKVRWQRLEDKTEEGGLAPNCPVVSLSGQRCHIWDFMQ 1
2 GNRPLVLNFGSCTuPSFIFKFDQFKRLIEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVDIKNHQNLQDRLRAAHLLLDRSPQCPVVVDTMKNQSSRLYAALPERLYVLQAGRILYK 0
0 GKPGPWNYHPEEVRAVLEKLHS* 0

>DIO1_vicVic Vicugna vicugna (vicugna)
0 MGLSLPGLWLKRLWVLFQVVLHVALGKVLLTLFPGRVKQDILAMSQRTGMAQNPHFSYENWIPTFFSTQYFWFILKVRWQRLEDTTEQGGLAPDCPVVCLSDRVHIWDFMQ 1
2                     FDQFKRLIEDFNSIADFLIIYIEEAHAS 1
2 DGWAFKNNVDIKNHRNLQDRLRAAHLLLDRSPQCPVVVDTMKNQSSQLYAALPERLYVLQKGRILk 0
0 GKPGPWNYHPEEVRAVLEKLHS* 0

>DIO1_eriEur Erinaceus europaeus (hedgehog)
0 MGLPSPGLWLKRLWVLFQVALHVAIGKVLLTLFPRRVKQNILAMGEKTGMTRNPHFSHENWIPTFFSTQYFWFILKVRWQRLEDMTEQGGLAPNCPVVRLSGERCSIWDFMQ 1
2 1
2 DRWAFKNNVDIRTHRNLQDRMRAALLLLDRDPQCPVVVDTMENQSSQLYAALPERLYVLQEGRILYK 0
0 GKPGPWDYQPQEVRAVLEKLRGKCGQTLPKL* 0

>DIO1_sunMur Suncus murinus (shrew)
0 MGLPGLGLLLKRFGVLVRVALKVAVGKVLLTLWPSAIRPHLLAMSEKTGMAKNPRFTYEDWAPTFFSTQYFWFVLKVNWQQLEDRTKQGDIAPDSPVVHLSGQRARLWDFMQ 1
2 GNRPLVLNFGSCSuPSFLFKFDQFKRLVEDFSSVADFLTVYIEEAHAS 1
2 DGWAFKNNVDIRRHRDLQERLQAARLLLDRNPGCPVVVDTMENRSSQLYAALPERLYVLQEGRILYK 0
0 GGPGPWNYHPEEVHAVLEQLCRSSAQSPRL* 0

>DIO1_loxAfr Loxodonta africana (elephant)
0 MGLPQPGLWLKRLWIFLKVALHVAMGKVLLILFPGRVKKNILAQNPHFAYDMWGSTLFSIPYFWFILKVYWQRLEDKTEEGGPAPDCPVVCLSGQRCNISDFMQ 1
2 GIRPLVLNFGSCTuPSFLSKLDQFKRLVEDFSSMADFLIIYIEEAHAT 1
2 DGWAFKNNVAIRNHRNLQDRLQAAHLLLDRSPQCPVVVDTMQNVSSQLYAALPERLYVLQEGRILYK 0
0 GKPGPWNYHPEEVRAVLEKLNS* 0

>DIO1_proCap Procavia capensis (hyrax)
0 MGLPQPVLWLKRLWVLLRVALHVAMGKVLLALFPGRVKKNILAQNPHFAYDMWCSTLFSVPYFWFVLKVYWQRLEDKTEEGGLAPNCPVVHLSGQRRNIWDFMQ 1
2 GIRPLVLNFGSCTuPSFLSKLDQFKRLVEDFSSMADFLIIYIEEAHAT 1
2 DGWAFKNNVAIRTHRNLQDRLQAARLLLDRSPQCPVVVDTMQNVSSQFYAALPERLYVLQEGRILYK 0
0 GKPGPWNYHPEEVRAFLEKFGS* 0

>DIO1_echTel Echinops telfairi (tenrec)
0 1
2 GTRPLVLNFGSCTuPSFLLKFDQFKRLMDDFHATADFLI 1
2 DGWAFKNNVTIRTHQNLEDRLRAARLLLDRGPQCPVVVDTMENESGRLYAALPERLYVLQEGRILYK 0
0 GKPGPWNYRPEEVRAVLEKLDS* 0

>DIO1_choHof Choloepus hoffmanni (sloth)
0 MGLSWPGLWLKRLWVLLQVALHVAMGKILLTLFPGRVKQNILAMSRRANNTKDPQFPYDNWGPTFFNTQYFWFVLKVRWQRLEDKTEQGGLAPNCPVVHLSRQRCNIWDFMQG 1
2 GNRPLVLNFGSCTuPSFLFKLDQFKRLVDDFSSIADFLIIYIEEAHAS 1
2                    RRRAARLLLDRSPQCPVVVDTMQNQSSQLYAALPERLYMLQEGRILYK 0
0* 0

>DIO1_dasNov Dasypus novemcinctus (armadillo)
0 MGLSQPGLWLKRLWILFQVALHVAVGKTLLTLFPGRVKQNILAKSQKSHKAENPHFPYDNWGPTFFNTQYFWFLLKIGWQRLEDKTEQGGLAPNCPVVHLSGQRCNIWDFMQ 1
2 GNRPLVLNFGSCTuPSFLFKFDQFKRLVEDFSSIADFLIIYIEEAHAS 1
2 DGWAFKNNVDIRNHRNLQDRQRAACLLLDRSPQCPVVVDTMQNQSSQLYAALPDRLYVLQEGRILYK 0
0 GKPGPWDYQPEEVRAVLETLNG* 0

>DIO1_monDom Monodelphis domestica (opossum)
0 MAELLRLWLWLWLRLQRLWVLLQVVGHVLMGKLMKMLSPDRMKQHILGMGQKSSIFQNPNFKYENWGPTFFTLPYFLFVLRVRWQRLEDQALQGGPAPDCPVVSLRGQPRRLWDFMH 1
2 ANRPLVLSFGSCTuPSFIFKFDQFHRIMEDFSSVADFLIIYIEEAHAT 1
2 DGWAFANNIDIKQHRTLQDRMEAARLLMDRKPLCPIVVDTMDNLATRKYAALPERFYILLEGHILYK 0
0 GGPGPWSYNPEEVRAVLETLSR* 0

>DIO1_triVul Trichosurus vulpecula (possum)
0 MAGPLLWVRRFWALLQVAFHVVVGKLLKTLFPNMMKKHILSLGQRSSISQNTQFAYENWGPTFFSIQYFFFVLKVRWQRLEDQALQGGLAPNPPVVTLKGESRHIWDFMH 1
2 GNRPLVLNFGSCTuPSFILKFDQFRKLIEDFSSIADFLIIYIEEAHAA 1
2 DGWAFENNIDIKQHRTLQDRRETAQLLMDRKPLCPIVLDTMDNLTSKKYAALPERFYVLLEGRILFK 0
0 GDPGPWNYHPEEVQAVLQ* 0

>DIO1_ornAna Ornithorhynchus anatinus (platypus)
0 1
2 GSRPLVLTFGSCTuPSFLFKFDQFNQLVQDFNSIADFLIIYIEEAHPT 1
2 DGWAFANNVDIPSHRSLQERQEAARRLLARGPRCPVVVDTMDNASSRQYAALPERLYLLREGKVVYK 0
0 GGPGPWNYNPGEVRAVLEKLS 0*

>DIO1_galGal Gallus gallus (chicken)
0 MLSIRVLLHKLLILLQVTLSVVVGKTMMILFPDTTKRYILKLGEKSRMNQNPKFSYENWGPTFFSFQYLLFVLKVKWRRLEDEAHEGRPAPNTPVVALNGEMQHLFSFMR 1
2 DNRPLILNFGSCTuPSFMLKFDEFNKLVKDFSSIADFLIIYIEEAHAV 1
2 DGWAFRNNVVIKNHRSLEDRKTAAQFLQQKNPLCPVVLDTMENLSSSKYAALPERLYILQAGNVIYK 0
0 GGVGPWNYHPQEIRAVLEKLK* 0

>DIO1_anoCar Anolis carolinensis (lizard)
0 MFKAGRLVLKTWLLLQVCLSTAVGKLFMILFPATAKRYILKQSERSSMGRNPNFVYENWGPTFFSFQYLLFVLKVKWKRLEDKALQGCPAPNTPVVDFDGKIHHILDFMQ 1
2 DNRPLVLAFGSCTuPSFMFRFGEFKKLIEDFSFAADFLVIYIEEAHAS 1
2 DGWAFKNNIVIKSHRTLHDRMQAAEILLKQYPLCSVVMDTMENLSSSTYAALPERLYVLQGGNIVYK 0
0 GGVGPWNYNPQEVREVLEKL* 0

>DIO1_xenTro Xenopus laevis (frog) tga confirmed
0 MLRYIQKALILFFLFLYVVVGKVLMFLFPQTMASVLKSRFEISGVHDPKFQYEDWGPTFFTYKFLRSVLEIMWMRLEDEAFVGHSAPNTPVVDLSGELHHIWDYLQ 1
2 GTRPLVLSFGSCTuPPFLFRLGEFNKLVNEFNSIADFLIIYIDEAHAA 1
2 DEWALKNNLHIKKHRSLQDRLAAAKRLMEESPSCPVVLDTMSNLCSAKYAALPERLYILQEGKIIYK 0
0 GKMGPWGYKPEEVCSVLEKKK* 0

>DIO1_danRer Danio rerio (zebrafish)
0 MGSAVGFALRKLFVYISAVLMVCAAILQMSMLKLLSFISPGRMRKIHMKMGERSTMTQNPKFRYEDWGPAFFSLAFIKTLFFVNWCSLGLEAFEGHAAPDSALITLDRQKTSVHRFLK 1
2 GNRPLVLSFGSCTuPPFLYKLDEFKQLVKDFSNVADFLIVYLAEAHAT 1
2 DAWAFKNNVDISVHKNLEERLAAARTLLKEDPPCPVVVDEMNNITASKYGALPERLYVIQSGKVIY 0
0 QASDLGGQA* 0

>DIO1_takRub Takifugu rubripes (fugu) genome glitches
0 MLLQKLAMYLSTAGLFCFMITLNVVLWILNIVAPALAKKIALKMGEKATMTQDPLFKYEDWGLTFASTALVKTASRHMWLSLGQEAFAGLEAPDSPVVTMERKRSSIGEFMK 1
2 TNRPLVLNFGSCTuPPFMFKLEEFKQLVRDFSDVADFLVVYIAEAHST 1
2 DGWAFKNNFDINQHRNLEDRLSAAQILVQKDPLCPVVVDDMNNSCAIKYGALPERLYVLQAGKVLYK 0
0 GAVGPWGYDPREVRSYLEKMK* 0

>DIO1_calMil Callorhinchus milii (elephantfish) weak match but introns ok
0 MWRRVVVYMQTVLLLVCICVRVAVGRVMLTLFPATTRRLELRNGLKTTMTLNPRFRFEDWGPSMFSLSSLRAVTTSIIANSGDRAFPGQPAPDTTLIDLDNTAHTIRSFIR 1
2 1
2 0
0 SGLGPWGYKPEEVRGVLHTLE* 0

>DIO1_braFlo Branchiostoma floridae (amphioxus) fused terminal exons frag
0              FTSTSFWTMWESEVMKDLQCETRVGGKAPDAPLLHMDGLTQARLMDFAMK 1
2 PGRPLVVNFGSCTuPPFMAHLAKFNDMVAEFADRADFLTVYIAEAHAS 1
2 RPHSSVTFTVFRKHQILEERLVAAKMLVDSNVSGGPLLVDTMDDVANQKYGAWPERLYILLDGRVMYK 00 GMPGPHGYHVHQVRDWLEKYLRNN* 0

DIO2: 27 vertebrate iodothyronine deiodinases

>DIO2_homSap Homo sapiens (human) iodothyronine deiodinase type II 2 exons chr14 uc001xuu.1
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSSAEGGDNSGNGTQEKIAEGATCHLLDFASPERPLVVNFGSATuPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDG
WAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKRuKKTRLAG* 0

>DIO2_macMul Macaca mulatta (rhesus)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSSAEGGDNSGHGTQEKIAEGAACHLLDFASPERPLVVNFGSATuPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDG
WAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKRuKKTRLAG* 0

>DIO2_otoGar Otolemur garnettii (bushbaby)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 0
0 VKLGEEAPNSSVVHVSNPEKGDSSGNGAPENAAAECHLLDFASPERPLVVNFGSATuPPFTSQLPAFRKLVEEFSAVADFLLVYIDEAHPSDG
WAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFFYNLQEVRRWLEKNFSKRuNRLAG* 0

>DIO2_tupBel Tupaia belangeri (tree_shrew)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSNLEGGHNGGNGTQEKTADGAECHLLDFANSERPLVVNFGSATuPPFTSQLPAFSKLLEESSVADFLVVYIDEAHPSDG
WAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFYYNLQEVRRWLEKNFSKRuNSLAG* 0

>DIO2_ratNor Rattus norvegicus (rat)
0 MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGLRCVWNSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSNPEAGNNCASEKTADGAECHLLDFACAERPLVVNFGSATuPPFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDG
WAVPGDSSMSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKRuILD* 0

>DIO2_canFam Canis familiaris (dog)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGMRCIWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSNSEGGDNSRNGAQVKIVDGAECHLLDFASPERPLVVNFGSATuPPFTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGD
SSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFYYNLQEVRRWLEKNFSKRuNRLAG* 0

>DIO2_bosTau Bos taurus (cow)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGQWRRMLTSEGMRCIWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSSPEGGDTSGNGAQEKTVDGTECHLLDFASPERPLVVNFGSATuPPFTNQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGD
SSLFFEVKKHRNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFFYNLQEVRRWLEKNFSKRuKRLAG* 0

>DIO2_susScr Sus scrofa (pig) tga tag
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGMRCIWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSNPEGSNNHGHGTQEKTVDGAECHLLDFANPERPLVVNFGSATuPPFTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDG
WAVPGDSSLSFEVKKHQNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFYYNLQEVRRWLEKNFSKRuKLD* 0

>DIO2_equCab Equus caballus (horse)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGMRCIWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSNPDRGDNRGNGAQGKTVDGTECHLLDFASSERPLVVNFGSATuPPFISQLPAFSKLVEEFSSVADFLLVYIDEAHPSDG
WAVPGDSSLSFEVKKHQNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFYYNLQEVRRWLEKNFSERuNKLG* 0

>DIO2_sorAra Sorex araneus (shrew)
0 MGNLSVHLLITLQILPVFFSNCLFLALYDSVILLKHVVLMLSRSKSTRGEWRRMLTSEGMRCIWKSFLLDAYKQ 0
0 VKLGEDAPNSSVVHVSSSEDGSRNGAHEKTVDGAECHLLDFASPERPLVVNFGSATuPPFTSQLPAFSKLVEEFSSVADFLLVYIDEAHPSDG
WAVPRDSSLSFEVKKHRSQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFCYNLQEVRDWLEKNFSKRuNSL* 0

>DIO2_echTel Echinops telfairi (tenrec)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVLLLKHVMLLLSRSKSTCGEWRRLLTFEGLRCVWKSFLLDAYKQ 0
0 VKLGEDAPNSGVVHVSNFEGGGNRGPGAQEKTADGAQCHLLDFARAERPLVVNFGSATuPPFTSQLPAFSQLVEEFSSVADFLLVYIDEAHPSDG
WAVPGDSSSSFEVKKHRNQEDRCAAAHRLLERFSLPPQCLVVADRMDNNANVAYGVAFERVCIVQQKIAYLGGKGPFCYNLQEVRCWLEKNFSKRuTRVAGQ* 0

>DIO2_dasNov Dasypus novemcinctus (armadillo)
0 MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 0
0 VKLGEDAPNSGVVHVSNPEGGDNSGNSAQEKTEDGAECHLLDFASPERPLVINFGSATuPPFTSQLPAFSKLIEEFSSVADFLLVYIDEAHPSDG
WAVPGGSSLSFEVKKHRNQEDRCAAVHKLLDRFSLPPQCHVVADRMDNNANIAYGVAFERVCIVQRQKIVYLGGKGPFCYNLQEVQHWLEKNFSKRuNRLAG* 0

>DIO2_monDom Monodelphis domestica (opossum) tga tag
0 MGLLSIDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 0
0 VKLGGDAPNSSVIHITSAEVGSGQQNNSRWKRFDGAECHLLDFANPERPLVVNFGSATuPPFTSQLPAFSKLVEEFSTVADFLLVYIDEAHPSDG
WAVPGNSSVSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANIAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEKNFSKRuNPG* 0

>DIO2_ornAna Ornithorhynchus anatinus (platypus) tga tga then tag
0 MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWNSFLMDAYKQ 0
0 VKLGGDAPNSSVVHVANANGETSGGNSPKWKNFSGRYGAECHLLDFASSERPLVVNFGSATuPPFISQLPAFSKLVEEFSAVADFLLVYIDEAHPSDG
WAVPGEFSLPFEVRKHQNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNFSKRuNPD* 0

>DIO2_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01A7SZJ 454
GAECHLLDFASSERPLVINFGSATuPPQPAAGLQQAGEEFSTVADFLLVYI

>DIO2_galGal Gallus gallus (chicken) 2 exons
0 MGLLSADLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGLRCVWNSFLLDAYKQ 0
0 VKLGGEAPNSSVIHIAKGNDGSNSSWKSVGGKCGTKCHLLDFANSERPLVVNFGSATuPPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDG
WAAPGISPSSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNFSKRuNPLSTEDLSTDVSL* 0

>DIO2_anoCar Anolis carolinensis (lizard)
0 MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGLRCVWNSFLLDAYKQ 0
0 VKLGGEAPNSSVIHIAKGNDGSNSSWKSVGGKCGTKCHLLDFANSERPLVVNFGSATuPPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDG
WAAPGISPSSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPPFFYNLQEVRLWLEQNFRKRuNPNLPQEDMLNISPLK* 0

>DIO2_xenTro Xenopus tropicalis (frog) tga tag
0 MGLLSVDLLITLQILPGFFSNCLFLALYDSVVLVKHVLLQLSRSKSSQSQWRRMLTPEGLRCVWNSFLLDAYKQ 0
0 VKLGQDAPNSNVIQVSNKICKSVQRKLVGKCHLLDFASSERPLVVNFGSATuPPFISQLPAFSKLVEEFSSVADFVLVYIDEAHPSDG
WAAPGTTSYEVKKHRNQEERCAAASKLLEHFSIPPQCQVVADCMDNNANVAYGVSFERVCIVQKQKIVYLGGKGPFFYNIQEIRRWLELSFGKRuT* 0

>DIO2_neoFor Neoceratodus forsteri (lungfish) exons unknown
0 MGLLSVDLLITLQILPWFFSNCLFLALYDSVVLLKHVILLLSCSKSSRGEWRRMLTSEGLRTVWNSFLLDAYKQ 0
0 VKLGGDAPNSKVVRVTSGCCRRRSFSGKGESECHLLDFASSNRPLVVNFGSATuPPFISQLPTFRKLVEEFSDVADFLLVYIDEAHPADG
WAAPGVATKSFEVKKHRSQEERCVAAHKLLEHFSLPPQCQVVADCMDNNTNVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLKEVRHWLEQTYRKRuVPTCELIM* 0

>DIO2_gasAcu Gasterosteus  aculeatus (stickleback) cDNA taa stop
0 MGMASGGLRVTLQILPGFFSNCLFLALYDSVVLLKAVVSLLSCSRAAGRGARRRMLTSAGLRSVWRSFLLDAYKQ 0
0 VKVGLEAPNSKVVKVPGSPQRSSNLSNLISLPPGAMIANEVECHLLDFESSDRPLVVNFGSATuPPFISHLPAFRQLVEDFSDVANFLLVYIDEAHPSDG
WVAPALGSCSFDVPKHQNLEERLGAARKLIEHFSLPPQCQLVADCMDNNANVAYGVSNERVCIVKQRKIAYLGGQGPFFNLKDVQHWLEQNFGKRFSRTSAEKDMSHISKKGIIHQ* 0

>DIO2_oryLap Oryzias latipes (medaka) tga stop
0 MGSAGAELLVTLQILPGFFSNCLFMALYDSVVLLKRVVSLLSCSRSVSCGEWRRMLTSAGLRAIWNSFLLDAYKQ 0
0 VKVPESPRWSSSIKSMTSVPRGARAQTGDECRLLDFESSDRPLVVNFGSATuPPFISHLPAFRQLVEDFSDVANFLLVYIDEAHPSDG
WVAPQMGPCSFSFRKHQNLEERMGAARQLTEHFSLPPQCQLVADCMDNNANVAYGVSNERVCIVHQRKIAYLGGKGPFFYNLKEVRQWLEQLRQTVGPNTEE* 0

>DIO2_danRer Danio rerio (zebrafish) cDNA tga taa
0 MGLLSVDLLVTLQILPGFFSNCLFFVLYDSIVLVKRVVSLLSCSGSTGEWQRMLTTAGVRSIWNSFLLDAYKQ 0
0 VKLGEAAPNSKVVKVTGINRCWSISGKTHNQCHLLDFESPDRPLVVNFGSATuPPFISQLPVFRRMVEEFSDVADFLLVYIDEAHPSDG
WVGPPMENFSFEVRKHRNLEERMFAARTLLEHFSLPPQCQLVADCMDNNANIAYGVSYERVCIVQKNKIAYLGGKGPFFYNLKDVRRWLEKCYGKu* 0

>DIO2_tetNig Tetraodon nigroviridis (pufferfish) tag
0 MGMASEDLLITLQILPGFFSNCLFLALYDSVVLVRRVVSRLSCSRSAGAKEWRPMLTSAGLRSIWNSFLLDAYKQ 0
0 VKVPNGPRWSSISNMLPGANLRNGIECHLLDFESSNRPLVVNFGSATuPPFISHLPAFRQLVEDFSDVADFLLVYIDEAHPSDG
WEAPPMGPCSFNVRKHQNLEERLGAARKLIEHFSLPPQCQLVADCMDNNANVAYGVSNERVCIVQQRKIAYLGGKGPFFYNLKEVRQWLEHSYGKR* 0

>DIO2_takRub Takifugu rubripes (fugu) taa
0 MGMASEDLLITLQILPGFFSNCLFLALYDSVVLVRRVVSRLSCSRSAGPKEWRPMLTSAGLRSIWNSFLLDAYKQ 0
0 VKLGCEAPNSKLVKVPDGARWSSINNITNMLPGASLCNGIECHLLDFESSNRPLVVNFGSATuPPFISHLPAFRQLVEDFSDVADFLLVYIDEAHPSD
GWKAPPMGPISFNVRKHQNLEERIGAAQKLIEHFSLPPQCQLVADCMDNNANVAYGVSNERVCIVQQRKITYLGGKGPFFYNLKEVRQWLEQSYGPHLI* 0

>DIO2_funHet Fundulus heteroclitis (mummichog) tag tag
0 MGSASEDLLVTLQILPGFFSNCLFLALYDSVVLVKRVVALLSRSRSAGCGEWRRMLTSEGLRSIWNSFLLDAHKQ 0
0 VKLGCEAPNSKVVKVPDGPRWSSTVVPCGSRIQTGGECRLLDFESSDRPLVVNFGSATuPPFISHLPAFRQLVEDFSDVADFLLVYIDEAHPSDG
WVAPQMGACSFSFRKHQNLEERIGAARKLIEHFSLPPQCQLVADCMDNNANVAYGVANERVCIVHQRKIAYLGGKGPFFYSLKDVRQWLELSYGRR* 0

>DIO2_calMil Callorhinchus milii (elephantfish) no intron tga stop many extra aa subsequent
0 MGLLSMDLIMKLQILPGFSNCLFLAAYDSFVLLRQAVSLLSCSGLGPDPQHRMLTAEGMQVVWQSFLLDALKQ 0
0 VKVGLEAPNSAVARLDGGAPCRLLDFASRDRPLVVNFGSATuPPFVSRLPAFRQMVERYAEVADFLLVYVDEAHPSDGWALRSRFQLRRHRSQEER
CSAAGLLAREFGLPAACGVVADLMDNNANRAYGVAFERLCVVQSQKIAYLGGKGPFFYNLNGVREWLERHSGQRWG* 0

>DIO2a_petMar Petromyzon marinus (lamprey)
0 MTAELNVSVFVALRILPGFFTNCLFLGLRDVLALLARSTRALFARHVPASCCPCPPEACRRVLTRAGMRAVWRSFLLDARRE 0
0 AQRAGDPAPNPRVALLADARSSSPAAAAAIPCRLQELSREGRPLVINMGSASuPPFVGRLPEFRRVVDDFSHAADFLLVYVEEAHPSDGWAVPGALQVSRARSLENNNSMA

>DIO2b_petMar Petromyzon marinus (lamprey) frag
0 SRPVVDSLLILPGVFSNCLFLALYDAVSFLRRALQASLTHSAKGDAQHPRMLAGQGMLSVWRSYVLDAHKK
VRLGGEAPNSSV RPSPPQPPPPQLRQAPPCRLLDFARAHRPLVVNFGSASuPPFVEQLGEFCDLVRDFADVADFLVVYIEEAHPSDAWPAPGGLEVPRHLALGDRCVAASQLRGLMPPLGRCPVVADAMDNNANIDYGVSYERLYVIQDG
RIRYLGGKGPFFYRVREVKSFLESVKASR* 0

DIO3: 36 vertebrate iodothyronine deiodinases

>DIO3_homSap Homo sapiens (human) 1 exon chr14
0 MLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYAQGNRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGARPRRV* 0

>DIO3_macMul Macaca mulatta (rhesus)
0 MLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQRILDYAQGNRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGARPRRV* 0

>DIO3_musMus Mus musculus (mouse)
0 MLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLRRRHPDHPEPEVELNSEGEEMPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVRPDGFQSQRILDYAQGTRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYVIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGTRPHRF* 0

>DIO3_ratNor Rattus norvegicus (rat)
0 MLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLRRRHPDHPEPEVELNSEGEEMPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVRPDGFQSQRILDYAQGTRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYVIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGTRPRRL* 0

>DIO3_cavPor Cavia porcellus (guinea_pig)
0 MLRSLLLHSLRLCAQIASCLVLFPRFLGTAFMLWLLDFLCIRKHFLRRRGLGQPEPEAELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQRILDYEHGNRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGPRHGRF* 0

>DIO3_canFam Canis familiaris (dog)
0 MLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPAVELDSDGEELPPDDPPVCVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPNGFQNQHILDYARGNRPLVLNFGS
CTuPPFMARMSAFHRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAAQVLQQGAPSCSLVLDTMANSSSSAYGAYFERLYVIQNGTVMYQGGRGPDGYQVSELRTWLERYDEQLHGAQPRRV* 0

>DIO3_felCat Felis catus (cat)
0 MLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPAAELDSDGEEVPPDDPPVCVSDDNRLCTLASLKAVWHGQKuDIFKQAHEGGPAPNSEVVLPNGFQNQHILDYARGNRQLVLNFGR
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAAEVLQQGAPSCSLVLDTMANSSSSAYGAYFERLYVVQNGTIMYQGGRGPDGYQVSELRTWLERYDAQLRGGQPRRV* 0

>DIO3_bosTau Bos taurus (cow)
0 MLRSLLLHSLRLCSQTASCLVLFPRFLGTAFMLWLLDFLCIRKHLLGRRRRGQPEIEVELNSDGEEVPPDDPPVCVSDDNRLCTLASLRAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQNQHILDYARGNRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAARVLQQGAPECALVLDTMTNSSSSAYGAYFERLYIIQSGTIMYQGGRGPDGYQVSEVRTWLERYDEQLHGPQPRRV* 0

>DIO3_susScr Sus scrofa (pig)
0 MLHSLLLHSLRLCAQTASCLVLFPRFLGTACMLWLLDFLCIRKHLLGRRRRGEPETEVELNSDGDEVPPDDPPICVSDDNRLCTLASLRAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQNQHILDYARGNRPLVLNFGS
CTuPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAARVLQQGAPECSLVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDQQLHGPQPRRV* 0

>DIO3_loxAfr Loxodonta africana (elephant)
0 MLRCMLLHSLRLCAQTASCIVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPETEVELNSDGEEVPPDDPPISVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQRILDYVRGNRPLVLNFGS
CTuPPFMARMSAFQRLVTKYRRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQNGTIVYQGGRGPDGYQVAKLRTWLERYDEQLHSAQTRRV* 0

>DIO3_echTel Echinops telfairi (tenrec)
0 MLRSPVLHSLVLCAQDAYSIVLFPCLLGTAFMQRQDFLCIRKHFLGLRRRGQPEPEVELNSDGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYARGSRPLVLNFGS
CTuPPFMARMSAFQRLVSKYRGEVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAARVLQQGAPDCALVLDTMTNSSSSAYGAYFERLYVIQNGTVMFQGGRGPDGYQVAELRTWLQQYSEQQHRAQPRRV* 0

>DIO3_monDom Monodelphis domestica (opossum) tga taa
0 MRSRREGDTEEPRKAGKGGGGGMGDEEDGAAVIQSPGLPPDDPPVWVSDSNRLCTLESLKAVWHGQKMDFFKTARKGSLAPNPEVIQLDGLKRLRILDFARGSRPLVLNFGS
CTuPPFMARLRAFQRLASTFLDIADFLLVYIEEAHPSDGWVSSDAAYDIPKHQCLQDRLRAARLLQEGVPGCLLAVDTMDNASSAAYGAYFERLYIIQDARVMYQGGRGPEGYKISELRHWLDQYKDRLQSPEASVVLPV* 0

>DIO3_ornAna Ornithorhynchus anatinus (platypus)
DCLLVYIEEAHPSDGWVSSDAPYDIPKHRCLQDRLRAARLMQRGAPGCRLAVDTMDNAASAAYGAYFERLYVVQDARVVYQGGRGPEGYKISELRLWLEQYRLRLRGPGPATAAAAAVLDV* 0

>DIO3_galGal Gallus gallus (chicken)
0 MLHSLGAHTLQLLTQAAACILLFPRFLLTAVMLWLLDFLCIRKKMLTMPTAEEAAGAGEGPPPDDPPVCVSDSNRMFTLESLKAVWHGQKLDFFKSAHVGSPAPNPEVIQLDGQKRLRILDFARGKRPLILNFGS
CTuPPFMARLRSFRRLAADFVDIADFLLVYIEEAHPSDGWVSSDAAYSIPKHQCLQDRLRAAQLMREGAPDCPLAVDTMDNASSAAYGAYFERLYVIQEEKVMYQGGRGPEGYKISELRTWLDQYKTRLQSPGAVVIQV* 0

>DIO3_xenTro Xenopus laevis (frog)
0 MLHCAGPHTGKLVKQVAACCLLLPRFLLTGLMLWLLDFQCIRRRVLLTAREESTAEHEDPPLCVSDSNRMCTVESLRAVWHGQKLDYFKSAHLGCSAPNTEVVMLEGRRLCKILDFSQGKRPLVVNFGS
CTuPPFMARLQAYRRLAAQHVGIADFLLVYIEEAHPSDGWLSTDASYQIPQHQCLQDRLAAAQLMLQGAPGCRVVVDTMDNSSNAAYGAYFERLYIVLEGKVVYQGGRGPEGYKISELRMWLEQYQQGLMGTKGSGQVVIQV* 0

>DIO3_ranCat Rana catesbiana (bullfrog)
0 MLPAPHTCCRLLQQLLACCLLLPRFLLTVLLLWLLDFPCVRRRVIRGAKEEDPGAPEREDPPLCVSDTNRMCTLESLKAVWYGQKLDFFKSAHLGGGAPNTEVVTLEGQRLCRILDFSKGHRPLVLNFGS
CTuPPFMARLQAYQRLAAQRLDFADFLLVYIEEAHPCDGWLSTDAAYQIPTHQCLQDRLRAAQLMLQGAPGCRVVADTMTNASNAAYGAYFERLYVILDGKVVYQGGRGPEGYKIGELRNWLDQYQTRATGNGALVIQV* 0

>DIO3_neoFor Neoceratodus forsteri (lungfish) 1 exon
0 MYQSSGVHTMNEVLKQAFACFILLPRFLVTALMLWLLDFLCVRRRVLLHMSRRQEASDLPDEPELCVSDSNRMFTLKSLRAVWHDQKLDFFKAAHIGLVAPNTEVIKLEGQRKAKILEFGGGKRPLILNFGS
CTuPPFMARLKAFRGVATQYKDVADFLLIYIEEAHPSDGWVSTDAPYQIPKHQCLEDRLKAAQLMNLEIPGCLVVVDTMDNASNAAYGAFFERLYIVQQERVVYQGGRGPEGYKISELKNWLDQYKSQLQNSSAVVIQV* 0

>DIO3a_danRer Danio rerio (zebrafish)
MEMLQGSAGVQSALKNAAVCVLLLPRFLLAALMLCLLDFLCIRRKLLLKMQEGAFSSPDDPPLRVSDSNKMFTLESLRAVWYGQKLDFFKSARLGGAAPNTEVFPLDGDARAAERILDYARGRRPLILNFGS
CSuPPFMTRLSAFQRVARQYADIADSLLVYIEEAHPSDGWVSSDAPVQIPRHRCLEDRLRAAQMLHREAAGSAVVVDSMQNSCNAAYGAYFERLYIVKDATVVYQGGRGPEGYRIAELRDWLERYRSGLQESAVLHV* 0

>DIO3bb_danRer Danio rerio (zebrafish)
0 MNTGRALKNALVCLLILPRFLVAAFMLWCLDFLCVRKRVLVHLQERAEEEEEDAEEEEEPLCISDSSRMFSWESLKAVFHGHKLDYMKSARLGHAAPDSEVFPLAEPRRGRVLEFARGHRPLVLSFGS
CSuPPFMRRLKAFRRLVLRYADVADALLIYIEEAHPSDGWRSSDAPHQIRRHRSLEERLSAARLMEREAPGCAVVADGMENAANSAYGAYFDRLYIVQDGRVVYQGGRGPEGFRISELRHWLDRYRERLRDAVIPV* 0

>DIO3bbb_danRer Danio rerio (zebrafish) bizarre ... matches opossum yet in both genomes
VHTLGFLTQVAACCFLGPRFLATAVTLWLLDFLCIRKKVLMRHRREGDAEDPDDPPIWVSDSNRFCTIESLKAVWHGQKMDFFKTAHKGSPAPNPEVIQLDGLKRLRILDFARGSRPLVLNFGS
CTuPPFTARLRAFQHLASTFLDIADFLLVYIEEAHPSDGWVTSDAAYDIPKHQCLQDRLRAARLLQEGAPGCLLAVDTMDNASSAAYGAYSERLYIIQEARVMYQGGRGPEGYKISELRHWLDQYKDRLQSPPASVVLPV* 0

>DIO3b_danRer Danio rerio (zebrafish)
0  GALKNALVCLLILTRFLVAAFMLWCLDFLCVRKRVLVHLQERAYAEQEEEPLCISDSSRMFSWESLKAVFHGHKLDYMKSARLGHAAPDSEVFPLAEPRRGRVLEFARGHRPLVLSFGS
CSuPPFMRRLKAFRRLVLRYADVADALLIYIEEAHPSDGWRSSDAPHQIRRHRSLEERLSAARLMEREAPGCAVVADGMENAANSAYGAYFDRLYIVQDGRVVYQ* 0

>DIO3a_takRub Takifugu rubripes (fugu) 1 exon
0 MLDSGGVQMATALKHAALCLMLLPRFLLTAVMLWLLDFLCIRRKVLLQMGQRQKSPDDPPVCVSDSNRMFTLESLGAVWYGQKLDFFKSAHLGSAAPNTEVMLVQERRQVRILDCMKGKRPLILNFGS
CSuPPFMTRLAAFQRVVRQYADIADFLVVYIEEAHPSDGWVSTDAPYQiPKHRCLEDRLRAAQLMLAEVPESNVVVDNMDNSSNAAYGAYFERLYIVRDERVVYQGGRGPEGYRISELRSWLEQYRNDVARSQTAVLHV* 0

>DIO3a_tetNig Tetraodon nigroviridis (pufferfish)
0 MLLPRFLLTAVALWLLDFLCIRRKVLLQMGQRQKSADDPPVCVSDSNKMFTLESLRAVWYGQKLDFLKSAHLGSAAPNTEVMLVQERRQVRILDCMKGKRPLVLNFGS
CSuPPFMTRLAAFQRIVRQYADIADFLVVYIEEAHPSDGWVSTDAPHQIPKHRCLEDRLRAAQLMLAEVPESNVVVDNMDNSSNAAYGAYFERLYILRDERVVYQGGRGPEGYRISELRTWLEQYRNDVAESQTAVLHV * 0

>DIO3a_gasAcu Gasterosteus aculeatus (stickleback)
0 MHDSGGGVRTARALKHAALCLMLLPRFLLAAVMLWLLDFLCIRKVLLKMGERQDSPDDPPLCVSDSNRMFTLESLRAVWYGQKLDCLKAAHLGRAAPNTEVMLVQQRRRVPILDCTKGKRPLILNFGS
CSuPPFMTRLAAFQRVVSQYADIADFLLVYIEEAHPSDGWVSSDAPYQIPKHRCLEDRLRAARLMLAEVPGSDVVVDNMDNSSSAAYGAYFERLYVVRDERVVYQGGRGPEGYRISELRAWLDEYRSELARSQTAVLHV* 0

>DIO3a_oryLap Oryzias latipes (medaka)
0 MDDSSGVQMARALKQAALCLMLLPRFLLAAVMLWLLDFLCIRRKLLLKMGERQDSPDDPPLCVSDSNKMFTLESLRAVWHGQKLDILKTAHLGQTAPNTEVVLVQERRQVRILDCMKGNRPLILNFGS
CSuPPFMMRLAAFQRVVSQYADIADFLVVYIEEAHPSDGWVSSDAPHQIPKHRCLEDRLRAAALMLTEVPGSKVVVDNMDNSSNAAYGAYFERLYVVRDETVVYQGGRGPEEYRISELKTWLQQYRKELLHSQNAVLHV* 0

>DIO3a_pimPro Pimephales promelas (minnow)
0 MEMLHGSAGVQTAKALKNAAVCLMLLPRFLLAALTLWLLDFLCIRRKLLMKMRESDIASPDDPPMCVSDSNKMFTLESLRAVWYGQKLDFFKTARLGGAAPNTEVVPLDSTRLRGTRRILDYARGRRPLIVNFGS
CSuPPFMTRLSAFQRVARQYADIADSLLVYIEEAHPSDGWVSSDAPYQIPRHRCLEDRLRAAELMNQKVPECAIVVDTMENSSNSAYGAYFERLYILKDEKVVYQGGRGPEGYRISELRDWLERYRNELEAS* 0

>DIO3a_ictPun Ictalurus punctatus (catfish)
0 MPDVQDFVKALKNALVSLMLLPRFLLTAVLLWFLDFVCIRRKVLVKMREREGSSPDDPSVTVSDSNRMFTVESLRAVWYSQKLDFCKTAHLGLTAPNSEVVPLGERKRARILDYARGSRPLILNFGS
CSuPPFMTRLAAFRRVADQYADIADSLLIYIEEAHPSDGWVSTDAPYQIPRHRCIKDRLRAARLMTATVPGSIVVVDTMDNSSNAAYGAYFERLYVVKDEKVVYQGGRGPEGYRIFEL NWLEPYRQKARGSRPIVGHV* 0

>DIO3a_spaAur Sparus aurata (seabream)
0 MHDSGGVQMARALKHAALCLMLLPRFLLAAVMLWLLDFLCIRKKVLLKMGERQDGPDDPPVCVSDSNKMFTLESLRAVWYGQKLDFLKSAHLGRTAPNTEVMLVQERRQVRILDCMKGKRPLILNFGS
CSuPPFMTRLAAFQRVVSQYADIADFLVVYIEEAHPSDGWVSSDAPYQIPKHRCLEDRLRAAQLMLAEVPGSNVVVDNMDNSSNAAYGAYFERLYIVRDERVVYQGGRGPEGYRISELRNWLEQYRNGLVNSQTAVLHV* 0

>DIO3a_parOli Paralichthys olivaceus (halibut) mRNA
MVHDSGGVQMARALKHAALCLMLLPRFLLAAVMLWLLDFLCIRKKVLLKMGERQDGPDDPPVCVSDSNKMFTLESLRAVWYGQRLDFFKSAHLGRAAPNTEVVLVQEGRQVRILDCMKGKRPLILNFGS
CSuPPFMTRLAAFQRVVSQYADIADFLVVYIEEAHPSDGWVSSDAPFQIPKHRCLEDRLRAAQLMLSEVPGGNVVVDNMDNSSNAAYGAYFERLYIVRDERVVYQGGRGPEGYQISGLRDWLEQYRSDLVNSKTPVLHV* 0

>DIO3b_takRub Takifugu rubripes (fugu) 1 exon N's
0 MNTIKSIKNALVFFVLLPRFLVAAVMFWLLDFLCIRKRVFFRMKEQEDDAVDPPLCISDSNRLFTVESLKAVWHGHKLDFLKAAHLGQGAPNTEVVQLEDQRRSRILDYAKDKRPLILNFGS
CTuPPFMARLKAFRGSWSKTQTSRLCSCVIEEAHPSDGWVSTDAPYQIPKHRCLADRLGAAQLMRLEVPGCLIVVDSMENSSNVAYGAYFDRLYILQEGKIVYQGGRGPEGYRITELRDWLVEYRESLKSSNDLVIHL* 0

>DIO3b_tetNig Tetraodon nigroviridis (pufferfish)
0 MNTMKSIKNALVFFVLLPRFLMAAVMFWLLDFLCIRKRVFFRMKEQEDDAVDPPLCISDSNRLFTVESLKAVWHGHKLDFLKAAHLGQGAPNTEVVQLEDQRRSRILDYAKDKRPLILNFGS
CTuPPFMARLKAFQGVVEQNADIADTVVVYIEEAHPSDGWVSTDAPYQIPKHRCLEDRLSAAQLMRLEVPGCLVVVDSMENSSNAAYGAYFDRLYILQEGKIVYQGGRGPEGYRITELRDWLEKYRKSLETSNNLVIHL* 0

>DIO3b_gasAcu Gasterosteus aculeatus (stickleback)
0 MNTIKAIKNAIVCLALLPRFLMAAFMFWLLDFLCIRRRVFFRMEQQEGDAIDPPLCMSDSNRLFSLESLKAVWHGHKLDFLKAAHLGHGAPNTEVVHLGDQRRGHILDYAKEKRPLVLNFGS
CTuPPFMARLKAFQGVVQQNADIADSVVVYIEEAHPSDGWMSTDAPYQIPKHRCLEDRLNAAQLMHLEVPGCPLVVDSMENSSNAAYGAYFDRLYILQEGTIVYQGGRGPEGYRVTELRDWLDRYRQTLGKSSQLVMSV** 0

>DIO3b_oryLap Oryzias latipes (medaka)
0 MNTMKALKNAIVCLLLLPRFLLAAVVFWLLDFSCIRKRVFLRMKEQGGDATDPPLCISDSNRLFSVESLKAVWHGHKLDFLKAAHLGRGAPNTEVVHLEDQRCSRILDYAKDKRPLILNFGS
CTuPPFMARLKAFQGVVQQNADIADSLVVYIEEAHPSDGWMSTDAPYQIPKHRRLEDRLNAAQLMHLEVPGCLVVVDSMENSSNAAYGAFFDRLYILQEGKVVYQGGRGPEGYRISELRDWLDQYRKGMEKSNNLVINM* 0

>DIO3_squAca Squalus acanthias (spiny_dogfish) note CTU
0 MNLLKEAVAVLILLPRFLVTALMLWLLDILCIRKRLLSKSREQTEGNADDPPLCVSDTNRMFTLESLKAIWHGQKLDFFKSAHVGSPAPNPEVVQLQEQRKVRLLDYSRGARPLILNFGS
CTuPPFMARLKAFQRVAIQYADIADFLLVYIEEAHPSDGWVSTDAPYDIPRHRCLEDRLKAARLMHKENPSCLVVADTM

>DIO3_calMil Callorhinchus milii (elephantfish) note ATU not CTU
0 MKLLKEAMAVLVLLPRFIVTALTLWLLDILCIRKRLLCKLRARYEE AAELLGPGAGPPAPD HRMLTAEGMQVVWQSFLLDALKQVKVGLEAPNSAVARLDGGAPCRLLDFASRDRPLVVNFGS
ATuPPFVSRLPAFRQMVERYAEVADFLLVYVDEAHPSDGWALRSRFQLRRHRSQEERCSAAGLLAREFGLPAACGVVADLMDNNANRAYGVAFERLCVVQSQKIAYLGGKGPFFYNLNGVREWLERHSGQRWG* 0

>DIO3_braFlo Branchiostoma floridae (amphioxus) taa 1 exon closer match to DIO3 note YTU not CTU 10x trace coverage
0 MGCGHHSSLSPHSVERPNAGMLCEAVRPLKALAAPILFVVLCFAFFLLKTASIVLTFVAPAKKEDLLAKLLGREEQEQTSSFQYDIDEFLTWNSLKGLVYSQMVGIMKRARHGKPAPDPTLVLLDSVTEKTLLSFAVADRPLFVNFGS
YTuPPFVPDLAVFDEIVAEFGERVDFLLVYIEEAHPTDGWSFRAGPDITSHQSIGQRCTAARAMLANRTVLYNVAVDSMTNSANYQYGAFPDRVYIIRHGQVVYEGGKGPFKYSLQEARTWLQENIGQQKN* 0

SELM1 (SEP15): 6 vertebrate sequences

>SELM1_homSap Homo sapiens (human)
0 MAAGPSGCLVPAFGLRLLLATVLQA 0
0 VSAFGAEFSSEACRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKK 0
0 LYAGAILEVCGuKLGRFPQVQ 1
2 AFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI* 0

>SELM1_susScr Sus scrofa (pig)
0 MAAEPGGWLGPALGLRLLLATALQM 0
0 VSAFGAEFSSESCRELGFSSNLLCGSCDLLGQFDLLQLDPDCRGCCQEEAQFETKK 0
0 LYAGAILEVCGuKLGRFPQVQ 1
2 AFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLQR* 0

>SELM1_ornAna Ornithorhynchus anatinus (platypus) EG339469
0 MAAAEAAVMAPWLRLFLAATLQA 0
0 ISAYGTELSSEACRDLGFSSNLLCSSCDLLGQFSLTQLDPVCRGCCQEEAQFESRK 0
0 LYAGAILEVCGuKLGRFPQVQ 1
2 AFVRSDKPKLFRGLQIKYVRGSDPVLKLLDESGNIAEELSILKWNTDSVEEFLSEKLERI* 0

>SELM1_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01BKAWJ EUEMSW404CBHS5 454
0                 LGFSSNLLCSSCDLLGQFSLTQLDPSCRGCCQEEAQFESRK 0
0 LYAGAILEVCGuKLGRFPQVQ 1
2 AFVRSDKPKLFRGLQIKYVHGSDPVLKLLDESGNIAEELSILKWNTDSVEEFLSEKLERI* 0

>SELM1_petMar Petromyzon marinus (lamprey) cDNA EG333213 tga-sel taa-stop full
0 MAAVRPGLLLLLLKAVS 0
0 VYATELTSEACRDLGFSSNLLCSSCDLLSQFGLDQLDPGCKRCCQLEVEESALK 0
0 LYPGAVLEVCGuKLGRFPQVQ 1
2 AFVRSNKPSTFKGLTIKYVRGSDPVLKLLDESGNVAEELSITKWNTDSVEEFLSEKLERL* 0

>SELM1_eptBur Eptatretus burgeri (hagfish) BJ647169 tga-sel taa-stop
0         FGTRLGAGSFLALCLVS 0
0 VSAAELSSEVCRDRGFSSGLVCSSCDLLAQFDLHRLDPDCRSCCQPEVEEQEIKR 0
0 YAGAVLEVCGuKLGRFPQVQ 1
2 AFVKSNKPSTFKGLTTK 0
0 YVRGADPVLKLLDQDGNVAEELSITKWNTDSVEEFLSENLERL* 0

SELM2: 22 vertebrate sequences

>SELM2_homSap Homo sapiens (human) NM_080430 Selenoprotein M (uc003ajq.1) chr22
0 MSLLLPPLALLLLLAALVAPATAATAYRPDWNRLSGLTRARVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTQDIPFY 2
1 HNLVMKHLPGADPELVLLGRRYEELE 0
0 RIPLSEMTREEINALVQELGFYRKAAPDAQVPPEYVWAPAKPPEETSDHADL* 0

>SELM2_musMus Mus musculus (mouse)
0 MSILLSPPSLLLLLAALVAPATSTTNYRPDWNRLRGLARGRVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTEDIQLY 2
1 HNLVMKHLPGADPELVLLSRNYQELE 0
0 RIPLSQMTRDEINALVQELGFYRKSAPEAQVPPEYLWAPAKPPEEASEHDDL* 0

>SELM2_ratNor Rattus norvegicus (rat)
0 MNILLSPPPLLLLLAALVAPATSITTYRPDWNRLRGLARGRVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTQDIQLY 2
1 HNLVMKHLPGADPELVLLSRNYQELE 0
0 RIPLSQMTRDEINALVQELGFYRKSAPEAKVPPEYLWAPAKPPEDASDRADL* 0

>SELM2_canFam Canis familiaris (dog)
0 MRLPLPPPPLLLLLAALAAAVTTFRPDWNRLHGLARARVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTQDIPLY 2
1 HNLVMKHLPGADPELVLLGHHYEELE 0
0 RIPLSEMTREEINELVQELGFYRKAAPDEAVPPEYLRAPARPAEGAPDRADL* 0

>SELM2_bosTau Bos taurus (cow)
0 MHLPLPPPPLLLLLAAVAAATTTFRPDWNRLQGLARARVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTQDIPLY 2
1 HNLVMKHLPGADPELVLLGHRFEELE 0
0 RIPLSDMTREEINALVQELGFYRKASPDEPVPPEYLRAPARPAGDAPDHADL* 0

>SELM2_susScr Sus scrofa (pig)
0 MHLPPLSLPLLLLLAALAAATTTFRPDWNRLQGLARARVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTQDIPLY 2
1 HNLVMKHLPGADPELVLLGHRFEELE 0
0 RIPLSDMTREEINALVQELGFYRKAAPDDPVPPEYMRAPARPAEGAPDRADL* 0

>SELM2_ornAna Ornithorhynchus anatinus (platypus)
0 MLVCPERRSWVLIPPLSLLLLLPGLLAAFQPDWSRLQGLARGKVE 0
0 TCGGuQLNRLKE 0
0 VKAFVTEDIPLY 2
1 HNLVMKHLPGADPELVLLNFRYEELE 0
0 RIPLSHMTRAEINQLVQDLGFYRKAERDAPVPPEFQQAPAKTSDLREKVQPQETPKSEEQNHPDL* 0

>SELM2_galGal Gallus gallus (chicken)
0 MRRAALAALLLLLAAAAGIERRPPRGLARGKVE 0
0 TCGGuRLSRLPE 0
0 VKAFVSQDIPLY 2
1 HNLEMKHLPGADPELVLLSFRYEELE 0
0 RIPLSDMTREEINQLVQELGFYRKETPEAPVPEEFQFAPAKPLPTLTPRRAPAADGKTLSEQDKKDHPDL* 0

>SELM2_botIns Bothrops insularis (snake) PVPDAFQMA
..PLLWLPLLLLGLLSAVAPLRAVQLDRSRLQWLARGKVE 0
0 SCGGuRLNRLPE 0
0 VKAFLNEDLPLY 2
1 HNMDLKYLAGADPELILLNIKFEELQ 0
0 RIPLSDMSREEINQLMQELGFYRKDTPDSLFRCFPNGAC* 0

>SELM2_xenTro Xenopus laevis (frog)
0 MWLPLPLLLGLLQLQPILSYQIDWNKLERINRGKVE 0
0 SCGGuQLNRLKE 0
0 VKGFVTEDLPLY 2
1 HNLEMKHIPGADPELVLITSRYEELE 0
0 RIPLSDMKRDEINQLLKDLGFYRKSSPDAPVPAEFKMAPARASGDTKEDL* 0

>SELM2_danRer Danio rerio (zebrafish)
0 MWPLIFTALLPSVILTYEVNIEKLSGLARARVE 0
0 TCGGuQLNRMRE 0
0 VKAFVTQDIPLY 2
1 HNLVMKHIPGADPELVLLNHYYEELD 0
0 RIPLSEMTRAEINKLLAELGFYKKDHPEDQVPEEFRFSPAKDSPFEGRQSSTAAPETTEPSDSQHTDL* 0

>SELM2_ictPun Ictalurus punctatus (catfish)
0 MFSTWPLLWAAFLPCISLAYEVDWKKLDGLARAKVE 0
0 SCGGuQLNRLRE 0
0 VKAFVTQDIPFY 2
1 HNLVMKHIPGADPELVLLNHYYEELD 0
0 RIPLSHMTRTDINGLLEELGFYKKARAEDDVPEEFRFSPAKDSPFKEHHTRRAPANSDLAQEPQPENESPHKDL* 0

>SELM2_oncMyk Oncorhynchus mykiss (trout)
0 MWFFFFVSLLNCVSAYDVDLKKLDGLAKAKVE 0
0 SQSCGGuQLNRLRE 0
0 VKAFVTQDIPLY 2
1 HNLVMKHIPGADPELVLLNHYYEELD 0
0 RIALSDMTRSEINELLEKLGFYKKAQAEDQVPEEFRFSPAKDSPFKATPADNASSDSDAEAKHSDL* 0

MSRB1: 17 vertebrate sequences

>MSRB1_homSap Homo sapiens (human) SEPX1 (uc002cng.1) SELX SELR human chr16
0 MSFCSFFGGEVFQNHFEP 1
2 GVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRSEALK 0
0 VSCGKCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFVPK 1
2 GKETSASQGH* 0

>MSRB1_macMul Macaca mulatta (rhesus)
0 MSFCSFFGGEVFQNHFEP 1
2 GVYACAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRPGALK 0
0 VSCGKCGNGLGHEFLNDGPKPGQSRFuIFSSSLKFVPK 1
2 GKETSTSQGH* 0

>MSRB1_musMus Mus musculus (mouse) exon break just at stop codon NP_038787
0 MSFCSFFGGEVFQNHFEP 1
2 GVYVCAKCSYELFSSHSKYAHSSPWPAFTETIHPDSVTKCPEKNRPEALK 0
0 VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFVPK 1
2 GKEAAASQGH* 0

>MSRB1_ratNor Rattus norvegicus (rat)
0 MSFCSFFGGEVFQNHFEP 1
2 GVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHEDSVAKCPEKNRPEALK 0
0 VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK 1
2 GKEAPASQGD* 0

>MSRB1_cavPor Cavia porcellus (guinea_pig)
0 MSFCSFFGGEVFQNHFES 1
2 GIYVCAKCGYELFSSRSKYAHSSPWPAFTDTIHADSVAKCPEHNRPGALK 0
0 VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK 1
2 DKETSASQGH* 0

>MSRB1_canFam Canis familiaris (dog) frag
0 MSFCSFFGGEVFQNHFEP 1
2 GVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPERNSPEALK 0
0 VSCGKCGNGLGHEFLNDGPKPGKSRFuIFSSSLKFVPK 1
2 GKGTSGSQEA* 0

>MSRB1_bosTau Bos taurus (cow)
0 MSFCSFFGGEIFQNHFEP 1
2 GIYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRPGAIK 0
0 VSCGRCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK 1
2 AEETSASQGQ* 0

>MSRB1_equCab Equus caballus (horse)
0 MSFCSFFGGEIFQNHFEP 1
2 GIYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRPEALK 0
0 VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFVPK 1
2 GKESSASQGQ* 0

>MSRB1_loxAfr Loxodonta africana (elephant)
0 MSFCSFFRSEVFQNHFEP 1
2 GVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVGKHPEHNRPEALK 0
0 VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK 1
2 GKETSASQGK* 0

>MSRB1_monDom Monodelphis domestica (opossum) frag
2 GVYVCAKCGYELFSSRSKYHHSSPWPAFTETIHADSVSKRPESGRSEALK 0
0 VSCGKCGNGLGHEFINDGPKKGQSRFuIFSSSLKFVPKG 1

>MSRB1_ornAna Ornithorhynchus anatinus (platypus)
0 1
2 GTYVCARCGYELFSSRSKYEHSSPWPAFTETIHPDSVAKREEPGRPNAFK 0
0 VSCGKCGNGLGHEFLNDGPRRGQSRFuIFSSLKFIPK 1
2 GKDSQAAQDK* 0

>MSRB1_galGal Gallus gallus (chicken)
0 MSFCSFFGGEVFKDHFEP 1
2 GVYVCARCGYELFSSRAKYEHSSPWPAFTETIHEDSVAKRKERPGALK 0
0 VSCGKCGNGLGHEFLNDGPKRGQSRFuIFSSSLKFIPK 1
0 GKSPQEN* 0

>MSRB1_anoCar Anolis carolinensis (lizard)
0 MSFCAFSGGEIYQGHFEA 1
2 GMYVCSKCGFELFSSKSKYAHSSPWPAFTETIHDDSITKYLERPNAFK 0
0 VLCGKCGNGLGHEFINDGPKKGQSRFZIFSSSLKFVPK 1

>MSRB1_xenTro Xenopus tropicalis (frog)
0 MSFCSFFGGEVYKDHFKS 1
2 GIYVCSECNYELFSSRSKYQHSSPWPAFTETVHKDSISKYLERPNAYK 0
0 VSCGKCGNGLGHEFINDGPKKGQSRFuIFSSSLKFIPK 0
0 DKVDGEVQRE* 0

>MSRB1_danRer Danio rerio (zebrafish) tga confirmed
0 MSFCSFSGGEIYKDHFES 1
2 GMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWGAYK 0
0 VRCGKCGNGLGHEFVNDGPKHGLSRFuIFSSSLKFI
0 PKVKNEQQ* 0

>MSRB1_ictFur Ictalurus furcatus (fish)
0 MAFCSFKGGEIFKDHYEP 1
2 GIYVCVKCGYELFSSTSKYKLSSPWPAFTTTIHEDSVSKQEERPGALK 0
0 IRCGKCNNGLGHEFLNDGPKHGLSRFuIFSSSLKFV 0
0 PKDKGGQ* 0

>MSRB1_oncMyk Oncorhynchus mykiss (trout)
0 MSFCSFFGGEVFKDHFKT 1
2 GLYMCAQCGHQLFSSRSKYEHSSPWPAFTETILQDSVSKHEERPGAFK 0
0 VRCGKCGNGLGHEFVGDGPKKGLSRFuIFSSSLKFV 0
0 PKDKVDGQ* 0

>MSRB1_salSal Salmo salar (salmon)
0 MSFCSFFGGEVFKDHFKT 1
2 GLYVCAQCGHQLFSSRSKYEHSSPWPAFTETVLQDSVSKHEERPGAFK 0
0 VRCGKCGNGLGHEFVGDGPKKGLSRFuIFSSSLKFV 0
0 PKDKVDGQ* 0

MSRB2: 6 vertebrate sequences

>MSRB2_homSap Homo sapiens (human) CBS-1 PILB 5 exons chr10
0 MARLLWLLRGLTLGTAPRRAVRGQAGGGGPGTGPGLGEA 1
2 GSLATCELPLAKSEWQKKLTPEQFYVTREKGTEP 0
0 PFSGIYLNNKEAGMYHCVCCDSPLFS 2
1 SEKKYCSGTGWPSFSEAHGTSGSDESHTGILRRLDTSLGSARTEVVCKQ 0
0 CEAHLGHVFPDGPGPNGQRFCINSVALKFKPRKH* 0

>MSRB2_musMus Mus musculus (mouse)
0 MARLLRALRGLPLLQAPGRLARGCAGS 1
2 GSKDTGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEA 0
0 PFSGMYLNNKETGMYHCVCCDSPLFSSEKKYCSGTGWPSF 2
1 SEAYGSKGSDESHTGILRRLDTSLGCPRMEVVCKQ 0
0 CEAHLGHVFPDGPKPTGQRFCINSVALKFKPSKP* 0

>MSRB2_bosTau Bos taurus (cow)
0 MARLLRALRGLTLREAPGWAVRGRADCGGFRAGA 1
2 GSPQAAHPDTFPFHRGSKSEWQKKLTPEQFHVTREKGTEP 0
0 PFSGIYLNNKEPGMYHCVCCDSPLFS 2
1 SEKKFCSGTGWPSFSEAHGTSGSDESNTGILRRADTSLGPARTEVVCKQ 0
0 CEAHLGHVFPDGPGPAGQRFCINSVALRFKPRKH* 0

>MSRB2_canFam Canis familiaris (dog)
0 MGDSSTTLRFLIRKRHSPYDFVVKIDKSKPKEERSLTKHEL 0
0 PLTKSEWQKKLTPEQFYVTREKGTEP 0
0 PFSGVYLNNKESGMYHCVCCDSPLFS 2
1 SEKKYCSGTGWPAFSEAHGTSGSDERDTGILRRVDTSLGLTRTEVVCKQ 0
0 CEAHLGHVFPDGPGPSGQRFCINSVALKFKPRKH* 0

>MSRB2_ornAna Ornithorhynchus anatinus (platypus)
0 0
0      DWKKKLTPEQYCVTRGKGTEP 0
0 PFSGIHLNNTERGMYHCLCCDMPLFS 2
1 SEKKFCSGTGWPSFLEAHGTQGTDESQTGILRRPDHSLGLARTEVVCKR 0
0 CEAHLGHVFDDGPPPTGQRFCINSVALTFKPS* 0

>MSRB2_xenTro Xenopus laevis (frog)
0 MSRLLTGFRLLLRVREISFPSVTAQRLQAWSRVRMAQTGADL 1
2 GSLTRYDDSAVSTDWQKKLTPEQYYVTREKGTEL 0
0 PFSGIYLNNTEKGMYHCVCCSAPLFS 2
1 SEKKYNSGTGWPSFSEAYGAQGADESNTNVLRRLDNSLGSTGTEVICKE 0
0 CDAHLGHVFEDGPPPYGQRFCINSVALTFAPSSM* 0

MSRB3: 5 vertebrate sequences

>MSRB3_homSap Homo sapiens (human) ER retention signal KAEL* AY358229
0 MSPRRTLPRPLSLCLSLCLCLCLAAALGSAQS 1
2 GSCRDKKNCKVVFSQQELRKRLTPLQYHVTQEKGTES 2
1 AFEGEYTHHKDPGIYKCVVCGTPLFK 2
1 SETKFDSGS 1
2 GWPSFHDVINSEAITFTDDFSYGMHRVETSCSQ 0
0 CGAHLGHIFDDGPRPTGKRYCINSAALSFTPADSSGTAEGGSGVASPAQADKAEL* 0

>MSRB3_musMus Mus musculus (mouse)
0 MSAFNLLHLVTKSQPVAPRACGLPS 1
2 GSCRDKKNCKVVFSQQELRKRLTPLQYHVTQEKGTES 2
1 AFEGEYTHHKDPGIYKCVVCGTPLFK 2
1 SETKFDSGS 1
2 GWPAFHDVISSEAIEFTDDFSYGMHRVETSCSQ 0
0 CGAHLGHIFDDGPRPTGKRYCINSASLSFTPADSSEAEGSGIKESGSPAAADRAEL*

>MSRB3_canFam Canis familiaris (dog)
0 MSAFNLLHLVTKSQPVALRACGLPS 1
2 GSCRDKKNCKVVFSQQELRKRLTPLQYHVTQEKGTES 2
1 AFEGEYTHHKDPGIYKCVVCGTPLFR 2
1 SESKFDSGS 1
2 GWPSFHDVISSDAITFTDDFSYGMHRVETSCSQ 0
0 CGAHLGHIFDDGPRPTGKRYCINSASLAFTPAGGGTQGSSGPGGPAAGGRAEL*

>MSRB3_ornAna Ornithorhynchus anatinus (platypus) (uc001ssm.1
0 MSAFNLLHLVTKSQPGSLQACGLPS 1
2 GSRRDKRSCKVVFSQEELRKRLTPLQYHVTQEKGTES 2
1 AFEGEFTHHKAPGTYKCVVCGTPLFKSETKFDSGS 1
1 1
2 GWPSFYDVIGSDAITLTDDFSYGMHRVETSCSQ 0
0 CGAHLGHIFEDGPRPTGKRYCINSASLSFAAVEENIDKENGSGDSPIQPGKTEL*

>MSRB3_petMar Petromyzon marinus (lamprey) cDNA FD728382 tga-sel taa-stop full
0 MSWAARLLGCAAVRAAGRAFGSVHARRHIALSTTLV 1
2 ASCKDTRTCRVNFAEEELRKKLSPLEFHVTQEKGTEP 1
2 AFTGKFAEHKGKGTYGCVVCQAPLFA 1
2 GWPSFYNIIRANAVSHSKDRSHGMHRTEVTCGQ 0
0 CGAHLGHVFDDGPPPTGKRFCINSASLNFNPSAEEEGGAEAVEEAPVKPELuGIGGIV* 0

GPX3: 32 chordate sequences

>GPX3_homSap Homo sapiens (human) chr5 5 exons plasma glutathione peroxidase 3
0 MARLLQASCLLSLLLAGFVSQSRGQEKSK 0
0 MDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYuGLTGQYI 1
2 ELNALQEELAPFGLVILGfpcnqfgKQEPGENSEILPTLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRRQAALGVKRK* 0

>GPX3_panTro Pan troglodytes (chimp)
0 MARLLQASCLLSLLLAGFVSQSRGQEKSK 0
0 MDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYuGLTGQYI 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRRQAALGVKRK*  0

>GPX3_macMul Macaca mulatta (rhesus)
0 MARLLQASCLLSLLLAGFLPQSRGQDKSK 0
0 MDCHGGVSGTIYEYGALTIDGEEYIPFKQYIGKYVLFVNVASYuGLTGQYI 1
2 ELNALQEELAPFGLVLLGFPCNQFGKQEPGENSEILPSLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPVMRWHHRTTISNVKMDILSYMRRQAALGVKRK*  0

>GPX3_otoGar Otolemur garnettii (bushbaby)
0 MARLLRASCLLSLLLAGFVPPGRGQEKSK 0
0 TDCHGGMSGTVYEYGALTIDGEEYIPFKQYTGKYILFVNVASYuGLTGQYV 1
2 ELNALQEELAPFGLVILGFPSNQFGKQEPGENSEILATLK 2
1 HVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGSPGRLFWEPMKVHDIRWNFEKFLVGPDGIPVMRWHHRTTVSNVKMDILSYMRRHAAQGGTGK*  0

>GPX3_tupBel Tupaia belangeri (tree_shrew)
0 MARLLRASCLLSLLLAGFVPPGRGQEKSK 0
0 TDCHGGVSGTIYEYGALTLDGEEYIPFKQYAGKYILFVNVASYuGLTGQYI 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLK 2
1 YVRPGGGFVPNFQLFEKGDVNGDKEQKFYTFLK 0
0 NSCPPTSELLGTPSRLFWEPMKIHDIRWNFEKFLVGPDGIPIMRWYHRTTISNVKMDILAYMRRQAALGLRGK*  0

>GPX3_musMus Mus musculus (mouse)
0 MARILRASCLLSLLLAGFVPPGRGQEKSK 0
0 TDCHGGMSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTDQYL 1
2 ELNALQEELGPFGLVILGFPSNQFGKQEPGENSEILPSLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTAELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPVMRWYHRTTVSNVKMDILSYMRRQAALSARGK*  0

>GPX3_ratNor Rattus norvegicus (rat)
0 MARILRASCLLSLLLAGFVPPGRGQEKSK 0
0 TDCHGGMSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTDQYL 1
2 ELNALQEELGPFGLVILGFPCNQFGKQEPGENSEILPSLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTAELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPIMRWYHRTTVSNVKMDILSYMRRQAALGARGK* 0

>GPX3_cavPor Cavia porcellus (guinea_pig)
0 MARLLRASCLLPLLLAGLAPPGRGQEKAK 0
0 TDCHSAVSGTIYEYGALTIDGEEYIPFNKYRGKYILFVNVASYuGLTDQYL 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPSLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELVGSPNRLFWEPMKVHDIRWNFEKFLVGPDGRPIMRWYHRTTVSNVKMDILACMRRQASLMALGN* 0

>GPX3_oryCun Oryctolagus cuniculus (rabbit)
0 MARLLRASCLLSLLLAGFVPPGRGQEKSK 0
0 TDCHDGVSSTIYEYGALTIDEKEYIPFKQYADKYVLFVNVATYuGLTGQYV 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPALK 2
1 YVRPGGGFVPNFQLFEKGDVNGDKEQKVYTFLK 0
0 NSCPPTSELLGSPNRLFWEPMKMHDVRWNFEKFLVGPDGVPIMRWYHRATVSNVKMDILAYMRRQAAMGAKGK*  0

>GPX3_ochPri Ochotona princeps (pika) 3rd exon again ends in potential PALKu 0
0  0
1 tDCHGGMSGTIYDYGALTIDGEEYIPFKQYAGKYVLFVNVATYuGLTGQYV 1
2 ELNALQEEFAPFGLVILGFPCNQFGKQEPGTNSEILPALK 0
0 HVRPGGGFVPNFQLFEKGDVNGDKEQKVYTFLK 0
0 NSCPPTSELLGSHSSLFWDPMKNHDIRWNFEKFLVGPDGVPIMRWHHRTTISNVKMDILTYMRRQAALGVRGK*

>GPX3_canFam Canis familiaris (dog)
0 MARLLRASCLLSLLLAGFVPPSRGQEKSK 0
0 TDCHGGVSGTIYEYGALTINGEEYIPFKQYAGKYILFVNVASYuGLTGQYL 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPSLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGSPGRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVSTVKMDILAYMRRQAALAIKGK*  0

>GPX3_felCat Felis catus (cat)
0 MARLLRASCLLSLLLAGFVPPSRGQEKSK 0
0 TDCHAGGSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTGQYV 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPSLK 2
1 HVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGSPNRLFWEPMKVHDIRWNFEKFLVGPDGVPLMRWYHRTTVSTVKMDILAYMRRQAALTVNGK*  0

>GPX3_oviAre Ovis aries (sheep)
0 MARLLRASCLLSLLLAGFIPPSQGQEKSK 0
0 MDCHAGVGGTIYEYGALTIDGEEYIPFKRYAGKYILFVNVASY*GLTGQYV 1
2 ELNALQEELEPFGLVILGFPCNQFGKQEPGENSEILATLK 1
2 YVRPGGGFTPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGSPGRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVNSVKMDILTYMRRRAVLEARGSN* 0
 
>GPX3_myoLuc Myotis lucifugus (microbat) frag
0 DCHAGVSGTIYEYGALTLNGEEYIPFKQYAGKYVLFVNVASFuGLTSQY 1
2 ELNALQEELQPFGLVILGFPCNQFGKQEPGENSEILPILK 2
1 VRPGGGFVPNFQLFEKGDVNGEKEQKVYTFLK 0

>GPX3_equCab Equus caballus (horse)
0 MARLLRASCLLSLLLAGFVPPSRGQEKSK 0
0 TDCHAGVSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTGQYV 1
2 ELNALQEEFAPFGLVILGFPSNQFGKQEPGENSEIPLTLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NACPPTSELLGSPDRLFWEPMKIHDIRWNFEKFLVGPDGKPIMRWYHRTTVNTVKMDILAYMRREAALAARGK*  0

>GPX3_myoLuc Myotis lucifugus (microbat)
0 TDCHAGVSGTIYEYGALTLNGEEYIPFKQYAGKYVLFVNVASFuGLTSQYV 1

>GPX3_pteVam Pteropus vampyrus (macrobat)
0 VDCHAGMSGTIYDYGALTIDGEEYIPFKQYAGKYVLFVNVATYuGLTGQYI 1

>GPX3_bosTau Bos taurus (cow)
0 MARLFRASCLLSLLLAGFIPPSQGQEKSK 0
0 TDCHAGVGGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTGQYV 1
2 ELNALQEELEPFGLVILGFPCNQFGKQEPGENSEILATLK 2
1 YVRPGGGFTPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELLGSPDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVNSVKMDILTYMRRRAVWEAKGK*  0

>GPX3_eriEur Erinaceus europaeus (hedgehog)
0 MARLLRVSCLLPLLLAGLVSPGQGQEKSK 0
0 TNCHPDVSGTIYDYGALTIDGEEYIPFKQYAGKYVLFVNVASYuGLTGQYI 1
2 ELNALQEELGPFGLVILGFPSNQFGKQEPGENSEILATLK 2
1 HVRPGGGFVPKFQLFEKGDVNGDKEQKVFTFLK 0
0 NSCPPTSELLGTSGRLFWEPMVHDIPSNSVNVLVGPKGINIIYLYHRITFTNVEWDILCYMNPQPSMGVKGQL 0

>GPX3_dasNov Dasypus novemcinctus (armadillo)
0 MALRAPCLLLLLLAGLVSPSQQEKSR 0
0 TDCHGGTSGSIYEYGALTLNGEEYIPFKQYAGKYILFVNVASFuGLTSQYL 1
2 ELNALQEELAPFGLVVLGFPCNQFGQQEPGENSEILPTLR 2
1 YVRPGQGFVPNFQLFEKWDVNADKEHKIYTFLN 0

>GPX3_loxAfr Loxodonta africana (elephant)
0 MARLLRASCLLSLLLAGFVPPSWGQEKSK 0
0 TDCHGGMSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTGQYI 1
2 ELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILASL 2

>GPX3_echTel Echinops telfairi (tenrec)
0 TDCHGGISGTIYEYGALTLDGEEYVPFKKYAGKYILFVNVASYuGLTGQYV 1
2 ELNALQEELEPFGLVILGFPSNQFGKQEPGENSEILASLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPTSELVGSPGRLFWEPMKIHDIRWNFEKFLVGPDGLPIMRWYHRTTVNTVKLDILHYMRQRAELRAQKK* 0

>GPX3_monDom Monodelphis domestica (opossum)
0 MAGGTLQVSWLLPLVLAAFVQPIWGQEKMK 0
0 TDCYPGITSTIYDYGALTIDGDEYIPFKQYAGKYILFVNVATYuGLTAQYL 1
2 ELNALQEDMKSSGLVILGFPCNQFGKQEPGQNSEILSALK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLK 0
0 NSCPPPSELIGTSSRLFWEPMRVHDIRWNFEKFLVGPDGIPIMRWYHRATVSTVKADILEYLQKQAA*  0

>GPX3_ornAna Ornithorhynchus anatinus (platypus) frag
2 TDCYSGVSGTVYEYGALTINGEEYIPFKQYMGKPILFVNVATYuGLTLQYL 2
1 ELNALQEELKAYGLVILGFPCNQFGKQEPGDNSEILPGLK 0
0 HVRPGGGFVPNFLLFEKGDVNGDKEQKVFTFLK 0
0 NSCPPPAELLGDPSRLFWSPMKTHDIRWNFEKFLVGPDGVPVMRWYHRATVSTVKADIVRYLRKLNNEQVEEEGRWKK* 0

>GPX3_galGal Gallus gallus (chicken)
0 MGCRAACVLAVLLAGLVPLGQGQEREK 0
0 VKCYDSVRGTIYDYGALTIDGDEYIPFRKYAGKMVLFVNVATYuGLTLQYL 1
2 ELNALQNELGPYGLVVLGFPSNQFGKQEPGQNSEILPALK 2
1 YVRPGGGFVPNFQLFQKGDVNGAKEQKVYSFLK 0
0 NSCPPVAEEFGNPKNLFWEPLRNHDIKWNFEKFLVGTDGVPVMRWYHRANIATVKNDIIAYMRQQRGQ* 0

>GPX3_anoCar Anolis carolinensis (lizard) frag
0 MRGRLKGPWIFPLLLAGLIQPNSAQERQKVRNHRT 0
0 VDCYDSIPGTVYNYGALSIHGDEYIPFQKYAGKLLLFVNVATYuGLTMQYL 1
2 ELNALQTELGRYGLVVLGFPCNQFGKQEPGENSEILPGLR 2
1 YVRPGGGFTPNFQLFQKGDVNGETEQRVYTFLK 0
0 NSCPPVIENFGDPNKLFWSPLKIHDIKWNFEKFLVGTDGKPVMRWYHRTNVATVKSDILRYMRKQLLYG* 0

>GPX3_xenTro Xenopus tropicalis (frog) frag
0 MGVKLRGLLMLPCFLAALIHAQNEMDQKS 0
0 VDCYSSIDGTIYDYGATTLDGTQFIPFKAYQGKYILFVNVATYuGLTMQYQ 1
2 ELNALQEELKNNNFVILGFPSNQFGMQEPGRNDEILPGLR 2
1 YVRPGGKFVPNFQLFEKGDINGRKEQKFYTFLK 0
0 NSCPPVGDNFGSATNRLMWEPIKVNDVKWNFEKFLVGPDGRPVKRWLPRTPVAQVRREIMSYMKLQPGTQRLLMLGLEQK* 0

>GPX3_danRer Danio rerio (zebrafish) frag
0 MGTQSNPWTSVVLLLALMHKIAALSNT 0
0 QACNSAAGDSFHNYGAKTINGTQFIPFSHYAGKHVLVVNVATYuGLTFQYV 1
2 ELNALHEELRHLGFTILGFPCDQFGKQEPGENNEILSALK 2
1 YVRPGNGFVPNFQLFEKGDVNGDGEQALFTFLK 0
0 NACPPVGESFGASNRLFWEPLKVNDIKWNFEKFLLDPDGRPVMRWFPRVNVSEVRADILKYFHQLLATAQ* 0

>GPX3_tetNig Tetraodon nigroviridis (pufferfish)
0 MADFFLQVALFALLGGCSSLETPLKQLCDSTS 0
0 PGTIYDYNAQVLNEDHCVDFSKFRGKPILFVNLATYuRYTHQYK 1
2 DLNALQEELGYTGFLILGFPSNQFGKQEPGAPSEILPGLK 2
1 YVRPGNGFVPNFQLFEKNDVNGENEQGVYTFLK 0
0 NSCPPVGGDLGNSGRLFWEPMKISDIKWNFEKFLVGPDGKPVMRWHPSIQVSEVRAD

>GPX3_takRub Takifugu rubripes (fugu) frag
0 0
0 NGTIYDYSAQVLNGPLKVDFSDFRGKFVLFVNVATYuSYTYQYK 1
2 DLNALQEELRDCGFIILGFPSNQFGKQEPGAPAEILPGLK 2
1 YVRPGKGFVPNFQLFSKDDVNGENEQGVYTFLK 0
0 NSCSPVGGDLGDHVGRLFWEPMRISDIKWNFEKFLVGPDGKPIMRWHPSIEVPEVRADIYKHLHENKILD* 0

>GPX3_gasAcu Gasterosteus aculeatus (stickleback)
0 MRPFLTLLLLGLLRPCGAETPLTQRCEF 0
0 TDGTIYNYHAKTLNESYTVNFSDLKGRSVLFINVATYuGYTFQYA 1
2 ELNALHEQMKPLGLTILGFPCNQFGKQEPGQPHEILPGLK 2
1 HVRPGNGFVPNFPLFEKGDVNGKDEQEVFTFLK 0
0 SSCPPVGDLLGDPARLFWGPIKLSDIKWNFEKFLVGPDGKPVMRWHPSVSVSEVKADIHKHLIQLYTQQL* 0

>GPX3_oryLap Oryzias latipes (medaka) frag
0 MRLHFLPQQLLLMMMMMRPMAHGIPLTQKCDSS 0
0 QKCDSSTDGSIYKYHAKTLNGSQTVNFSDYIGKSVLFVNVATYuGYTYQYV 1
2 ELNALHEELKPLGFTIVGFPCNQFGKQEPGQNHEILPGLR 2
1 HVRPGNGFVPNFLLFNKGDVNGKYEQDVFTFLK 0
0 GSCPPVGEDFGNTDRLFWEPLKIGDIKWNFEKFLVGPEGKPVMRWHPSVNVSTVRTDINFLLKLYTQDLFN* 0

>GPX3_calMil Callorhinchus milii (elephantfish) frag
2 ELNALHKELQPHGFTILAFPSNQFGKQEPGNNREILLSLK 2
1 YVRPGKGFVPNFLMFQKGDVNGVDEQEHYTYLK 0

>GPX3_leuEri Leucoraja erinacea (skate)
0 MKDTSKEWVLLSLLLAWIHNATSQT 0
0 AYCNSSASGSIYDYGAMAIDGSRYIPLREYMGKTVLILNVASFuGLTSQYV 1
2 ELNALHTDLKSHGFTILAFPCNQFGKQEPGTNQEILPLLK 2
1 YVRPGKGYVPNFQLFQKGDVNGEKEQGLFTYLK 0
0 GSCPPVGDNFGNMDRLFWQTLKISDIKWNFEKFLVGPDGRPVQRWHPLMPVPSVREDVTRYIRSLHRQQTIG* 0

>GPX3_braFlo Branchiostoma floridae (amphioxus) frag fused exons 2-3
0 EQCSCAAEQGSLHDHHAMLLDGSRNVSLAEYRGTTLLVVTVATFuGFTQQYVGLNALRN 12 KMVEENGHRFEILGFPTNQFGLEEPARNDEILNGIR 2
1 YVRPGNDYVPNFTMFQKGDCNGENEQSLFTYLK 0
0 SCCPPISDVMGISGNKDRLYWKPLKVNDVRWNFEKFLVDPEGRGVKR* 0

GPX5: 23 vertebrate sequences

>GPX5_homSap Homo sapiens (human)
0 MTTQLRVVHLLPLLLACFVQTSPKQEKMK 0
0 MDCHKDEKGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYP 1
2 ELNALQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPGLK 2
1 YVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLK 0
0 HSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK* 0

>GPX5_panTro Pan troglodytes (chimp)
0 MTTQLRVVHLLPLLLACFVQTSPKQEKMK 0
0 MDCHKDEKGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYP 1
2 ELNAPuEELKPYGLVVLGFPCNQFGKQEPGDNKEILPALK 2
1 YVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLK 0
0 HSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK* 0

>GPX5_macMul Macaca mulatta (rhesus)
0 MTTQLRVVHLLPLLLACFVQTSPKQETMK 0
0 MDCHKDEKGTIYDYEAIALNKNEYVPFKQYVGKHILFVNVATYCGLTAQYP 1
2 ELNALQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFSFLK 0
0 HSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK* 0

>GPX5_otoGar Otolemur garnettii (bushbaby)
0 MTAQLRVGYLFPFLLAGFVQTNPRMEKIK 0
0 MDCYKDVKGTIYDYDAFALNGKHYIQFKQYTGKHVLFVNVATYCGLTAQYP 1
2 ELNALQEELKPSGLVVLGFPCNQFGKQEPGDNAEILPGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGERELKVFTFLK 0
0 HACPHPSEILSSFKYISWEPIKVHDVRWNFEKFLVGPNGVPVMRWSHWATVSTVKSDILAYLKQFKTK* 0

>GPX5_musMus Mus musculus (mouse)
0 MVTELRVFYLVPLLLASYVQTTPRPEKMK 0
0 MDCYKDVKGTIYDYEALSLNGKEHIPFKQYAGKHVLFVNVATYCGLTIQYP 1
2 ELNALQEDLKPFGLVILGFPCNQFGKQEPGDNLEILPGLK 2
1 YVRPGKGFLPNFQLFAKGDVNGENEQKIFTFLK 0
0 RSCPHPSETVVMSKHTFWEPIKVHDIRWNFEKFLVGPDGIPVMRWFHQAPVSTVKSDIMAYLSHFKTI* 0

>GPX5_ratNor Rattus norvegicus (rat)
0 MAIQLRVFYLVPLLLASYVQTTPRLEKMK 0
0 MDCYKDVKGTIYNYEALSLNGKERIPFKQYAGKHVLFVNVATYCGLTIQYP 1
2 ELNALQDDLKQFGLVILGFPCNQFGKQEPGDNTEILPGLK 2
1 YVRPGKGFLPNFQLFAKGDVNGEKEQEIFTFLK 0
0 RSCPHPSETVVTSKHTFWEPIKVHDIRWNFEKFLVGPNGVPVMRWFHQAPVSTVKSDILAYLNQFKTI* 0

>GPX5_perMan Peromyscus maniculatus (deer_mouse)
0 MTMQLRVFYLVPLLLASYVQTTPRPEKMK 0
0 MNCYKDVKGTIYDYEALALNGKDRIQFKQYGKHVLFVNVATYCGLTIQYP 1
2 ELNALQEELKPAGLVVLGFPCNQFGKQEPGENIEILPGLK 2
1 YVRPGKGYVPNFQLFAKGDVNGEKEQKIFTFLK 0
0 RCPHPSEIVITHKHISWEPVKVHDIRWNFEKFLVGPDGVPVMRWFHQAPVSTIKADILAYLKQFRTKQG* 0

>GPX5_cavPor Cavia porcellus (guinea_pig) AAKN02051715
0 MTMQLRVFYLVPLLLASYVQTTPRPEKMK 0
0 MDCFKDVDDTIFNYEAVTLGRKELVHFSQFAGKLILVVNVATYCGLTSQYP 1
2 ELNALQEELKPFGLVVLGFPCNQFGKQEPGDNSEILPGLK 2
1 HVRPGGGFVPNFQLFEKGDVNGENEQKIFTFLK 0
0 RSCPHPSELVGSLRRVSWDPIRVHDIRWNFEKFLVGPDGVPVARWFHQAPVSAVREDILAYLKRPPRARGV* 0

>GPX5_oryCun Oryctolagus cuniculus (rabbit)
0 MAAQLRASHLVPFLLAGLVLTDSKPEKMK 0
0 MNCYKDVKGTIYNYEAFTLNGKQRIQFKQYAGKYVLFVNVATYCALTSQYP 1
2 ELNALQEELKPFGLVVLGFPCNQFGKQEPGQNSEILPALK 2
1 YVRPGKGFVPNFQLFEKGDVNGGKEQIFTFL 0
0 HSCPHPSGILGSLRHISWEPIEVHDIRWNFEKFLVGPDGVPVMRWFHLAEVSTVKSDILAYLKQSKPKK 0

>GPX5_ochPri Ochotona princeps (pika)
0 MAAQVQAFCLVPLLLAGFVQTNPNPEKMK 0
0 MDCDKGVEGTIFDYEAFTLDGTEKIRFRQYAGKYVLFINVATYCALTDQYP 1
2 ELNALQDELKPFGLVVLGFPCNQFGKQEPGQNSEILPALK 2
1 VRPGRGFVPNFQLFEKGDVNGRNEQSIFTFLK 0
0 HSCPHPSGTLGLLKHISWEPIKVHDIRWNFEKFLVGPDGVPVMRWFHLAEVSTVKSDILAYLKQSKPTEDAGAEGS* 0

>GPX5_canFam Canis familiaris (dog)
0 MTAWLGASYVLPILLVSFVQTNAKPEKTK 0
0 MDCYKDVKGTIYEYEALTLNGNERIQFKQYAGKHVLFVNVATYCGLTAQYP 1
2 ELNSLQEELKPLGLVVLGFPCNQFGKQEPGENSEILPGLK 2
1 YVRPGRGYVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 LSCPHPSEVLGSFRHISWDPVKVHDIRWNFEKFLVGSDGVPVLRWFHRTPISTVKEDILVYLKQLKMK* 0

>GPX5_equCab Equus caballus (horse)
0 MTAQLRASYLFLLLLAGFVQTENNAEKMK 0
0 TDCYKDVKDTIYEYSAVTLNGKEHIQFNQYAGKPVLFVNVATYCGLTAQYP 1
2 ELNTLQEELKPFDLVVLGFPCNQFGKQEPGENSEILPGLK 2
1 YVRPGGGYVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 HSCPHPSDVLGSLKHISWEPIKVHDIRWNFEKFLVGPDGVPVMRWFHKTPVSTVKSDILAYLKQFKIKK* 0

>GPX5_bosTau Bos taurus (cow)
0 MTIQLRASCLFLFFLAGFVQTNSNLEK 0
0 MDCYKDVKGTIYDYDAFTLNGKEHIQFKQYAGKHVLFVNVATYCGLTAQYP 1
2 ELNALQEELKPFGLVVLGFPCNQFGKQEPGENSEILPGLK 2
1 YVRPGGGYVPNFQLFEKGDVNGETEQKVFTFLK 0
0 QSCPHPSEIMGSIKHISWEPIMVRDIRWNFEKFLVGPDGIPVMRWFHRTPVSTVKTDILAYMKQFKTK* 0

>GPX5_susScr Sus scrofa (pig) CU313307
0 MTVQLGAFYLFPLFMAGFVQT 0
0 NSNLEKMDCYKDVTGTIYDYDAFTLNGNEHIQFKQYAGKHVLFVNVATYCGLTAQYP 1
2 ELNTLQEELKPFGLVVLGFPCNQFGKQEPGENSEILLGLK 2
1 YVRPGGGYVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 HSCPHPSELIGSIGYISWEPIRVHDIRWNFEKFLVGPDGVPVMRWVHETPISTVKSDILAYLKQFKTE* 0

>GPX5_eriEur Erinaceus europaeus (hedgehog)
0 MTVYLKASYLFPLLIASFVRASPKPEKMK 0
0 MDCYKDIKGSIYDYDALTLNGKERIQFKQYASKHVLFVNVATYCGLTTQYL 1
2 ELNALQEELKPLGLVVLGFPCNQFGNQEPGENSEILGLK 2
1 YVRPGRGYVPNFQLFEKGDVNRENEQKVFTLI 0
0 HSCPHPSQLLGSLKNISWEPVKVHDIRWNFEKFLVGPDGVPVMRWYHQTAVNTVKSDILTYMKQFKTK* 0

>GPX5_felCat Felis catus (cat)
0 MMAQLPASSLFPLLLVGVTCTNPKPEKVK 0
0 MDCYNDVKGTIYEYDALTLNGKEHIQFKQYAGKHVLFVNVATYCGLTAQYP 1
2 ELNALQDELKPWGLVVLGFPCNQFGNQEPGENSEILPGL 2
1 YVRPGRGYVPNFQLFEKGDVNGKTEQKVFSFL 0
0 LSCPQPSEVLGFTGHISWSPVKGHDIRWNFEKFLVGPNGVPVMRWFHQAPISTVRSDILEYLKQPKTK* 0

>GPX5_dasNov Dasypus novemcinctus (armadillo)
0 0
0 MYCYKDVKGTIYDYGAATLNGREYIQFRQYAGKHVLFVNVATYCGLTAQYP 2
1 2
0             SQNFEKGEWDSEGEQRIFTLK 0
0 HSCPHPSEILSSSRYISWEPMKVHDIRWNFEKFLVGPDGVPVMRWYHRTPVSTVRADILEYLKQFRTI* 0

>GPX5_loxAfr Loxodonta africana (elephant) gappy tandem
0  2
0 MNCYKDVKGTIYDYDALTLNGKEHIQFKKYAGKHVLFVNVATYCGLTAQYP 1
2 ELNALQEELKPFGLVILGFPCNQFGKQEPGENSEILPGLR 2
1 YVRPGGGYIPNFQLFEKGDVNGEEEQKIFTFLK 0
0 HSCPHPSDILGSSRYIFWEPMKVHDIRWNFEKFLVGSDGIPVMRWSHRAPVSTVKADILEYLKQLKTK* 0

>GPX5_echTel Echinops telfairi (tenrec)
0 MAGQLGASFLLPLFLAGFVQPSPKPEKMK 0
0 MHCDKDVKETIYNYGAFTLLGREYVNFNKFSGKLVLFVNVATYCGLTAQYL 1
2  2
1 YVRPGAGYVPNFQLFEKGDVNGINEQKVFSFLK 0
0 HSCPHPSFILGSPKYIFWEPVKVHDIRWNFEKFLVGPDGVPVMRWYHRAPVSAVRADILHYLKQLKTK*

GPX6: 26 vertebrate sequences

>GPX6_homSap Homo sapiens (human)
0 MFQQFQASCLVLFFLVGFAQQTLKPQNRK 0
0 VDCNKGVTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYuGLAAQYP 1
2 ELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLK 2
1 YVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDILEYLKQFNTH* 0

>GPX6_panTro Pan troglodytes (chimp)
0 MFRQFQASCLVLLFLVGFAQQILKPQNRK 0
0 VDCNKGVTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVATYuGLAAQYP 1
2 ELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLK 2
1 YVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSDLLGSSSQLFWEPMKVHDICWNFEKFLVGPDGVPVMRWFHQAPVSTVKSDILEYLKQFNTH* 0

>GPX6_macMul Macaca mulatta (rhesus)
0 MIRQFQACCLVLLFLVGFAQQTLKPQNRK 0
0 VDCNKRVTGTIYEYGALTLNSEEYIQFKQFAGKHVLFVNVATYuGLAAQYP 1
2 ELNALQEELKNFGVVVLAFPCNQFGKQEPGTNSEILLGLK 2
1 YVRPGSGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHRAPVSTVKSDILEYLKQFNAH* 0

>GPX6_otoGar Otolemur garnettii (bushbaby)
0 MIWQFWASCLFSLFLVGFTQQILKPEQMK 0
0 VDCNKDVAGTIYEYGALTLDGEYIQFKQYAGKHVLFVNVATYuGLAVQYP 1
2 ELNALQEDLRDFGVIILGFPCNQFGKEEPGKSPEILSGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGENEQKVFTFLK 0
0 NSCPPTSDVLGSSKHLFWEPMKVHDIRWNFEKFLVGPNGVPVMRWYHRAPVSKVKSDIQEYLKQDT* 0

>GPX6_tupBel Tupaia belangeri (tree_shrew) frag
0 ACSKGVAGTIYEYGALTLSGEEYIPFRQFAGKHVLFVNVATYuGLAAQYP 1
2 eLNALQEELGSRGLVVLGFPCNQFGKQEPGSNSEILSGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0          ELLGTSKQLWEPMKVDIRWNFEKFLVGPDGVPALRWFHKVPMSKVRSDILEYLERSDSQ* 0

>GPX6_musMus Mus musculus (mouse)
0 MAQKLWGSCLFSLFMAALAQETLNPQKSK 0
0 VDCNKGVTGTVYEYGANTIDGGEFVNFQQYAGKHILFVNVASFCGLTATYP 1
2 ELNTLQEELKPFNVTVLGFPCNQFGKQEPGKNSEILLGLK 2
1 YVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLK 0
0 NSCPPTSELFGSPEHLFWDPMKVHDIRWNFEKFLVGPDGVPVMRWFHHTPVRIVQSDIMEYLNQTSTQ* 0

>GPX6_ratNor Rattus norvegicus (rat)
0 MTQQFWGPCLFSFVMAVLAQETLDPQKSK 0
0 VDCNKGVAGTVYEYGANTLDGGEYVQFQQYAGKHILFVNVASFCGLTATYP 1
2 ELNTLQEELRPFNVSVLGFPCNQFGKQEPGKNSEILLGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGDNEQKVFSFLK 0
0 SSCPPTSELLGSPEHLFWDPMKVHDIRWNFEKFLVGPDGAPVMRWFHQTPVRVVQSDIMEYLNQTRTQ* 0

>GPX6_speTri Spermophilus tridecemlineatus (squirrel) frag
0  0
0 MDCNKGVTGTIYEYGALTLSGEEYIQFKKYAGKHVLFVNVATYuGLTAQYP 1
2 ELNTLQEELKPFNVTVLGFPCNQFGKQEPGKNSEILLGLK 2
1 YVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLK 0
0 NSCPPTSELLGSSKQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHQIPVSTVKLDILEYLKQFDTQ* 0

>GPX6_dipOrd Dipodomys ordii (kangaroo_rat) frag
0 MVQQFWAFYLFPLFLAGFAQETLKPQEMK 0
0 VDCDKGVTGTIYEYGALTLNGEEYIKFQQYAGKHVLFVNVATFuGLTAQYP 1
2 ELNALQEELKQSNVIVLGFPCNQFGKQEPGKNSEILSGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGENEQKVYTFLK 0
0 NSCPPTSELLGSSKQLFWEPMKIHDIRWNFEKFLVGPDGVPVMRWFHKIPVSTVKSDIIQYLNMSDTQ* 0

>GPX6_cavPor Cavia porcellus (guinea_pig) AAKN02051715
0 MHALLRAAGLGFPLLLLSLAQDTPEAQRMK 0
0 TDCNKTVPGTIYEYGALTLLGEEYIQFKQFAGKHVLFVNVATYuGLTAQYP 1
2 ELNALQDELRSFGVVLLGFPCNQFGRQEPRKNSEILSRLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSELLGSPRLLFWDPIRVHDIRWNFEKFLVGPDGVPVMRWFHKAPVSVVKSDILEYLKQFEAQ* 0

>GPX6_oryCun Oryctolagus cuniculus (rabbit) frag
0 MIPQFWASCLFLLFLVSFAQETPEPQKMK 0
0 MDCNKEVSGTIYEYGARTLNGEEDIQFQQYAGKYVLFVNVATYCANTAQYP 1
2  2
1 YVRPGGGFVPNFQLFEKGDVNGDKEQKFYTFLK 0
0 NSCPPTSELLGSPRHLFWDPLKIHDIRWNFEKFLVGTDGIPVMRWFHAAPVSTVKADILKYVEQFNTQ* 0

>GPX6_canFam Canis familiaris (dog)
0 MVPQLWASALLPLLLAGLAQPTQNTQKMK 0
0 VDCYNGVMGTIYEYGALTLNNEEYVQFKQYAGKHVLFVNVATYuGLTAQYP 1
2 ELNALQEELKSFGVVVLGFPCNQFGKQEPGKNSEILSGLK 2
1 HVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSDLLGSSNQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHQAPVSTVKADILAYLKQLKTE* 0

>GPX6_felCat Felis catus (cat)
0 MAPPLWASCLFPLFLGGLAQPAPKPQTMR 0
0 MDCYTGVTGTIYEYGALTLSDGDYIQFKQYAGKVVLFVNVASYCGLTAQYP 1
2 ELNALQDELKRYGVVVLGFPCNQFGKQEPGKNSEILSGLK 2
1 YVRPGGGFVPSFQLFEKGDVNGENEQKVFTFLK 0
0 SDILEYLKQLKTK* 0

>GPX6_equCab Equus caballus (horse)
0 MIRQLWASSLFPLFLVGFAQLTPESQKMK 0
0 MDCYKGVTGTIYEYGALTLNGEEYIQFKQYAGKHVLFINVATYuGLTAQYP 1
2 ELNALQEELKPFGVVLLGFPCNQFGKQEPGKNSEILSGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSDLLGSPKQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHRASVSTVKSDILEYLKQFTPE* 0

>GPX6_bosTau Bos taurus (cow)
0 MILQLWASCLFPLFLVGLAQLTPKQQQMK 1
0 VDCYKGVTGTIYEYGALTLNGEEYIQFKQYVGKHVLFVNVATYuGLTAQYP 1
2 ELNALQEELKPFGVVVLGFPCNQFGKQEPAKNSEILMGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NACPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWYHRASVSTVKSDMLEYLKQFKSE* 0

>GPX6_turTru Tursiops truncatus (dolphin) frag
0 MDCSKGVTGTICEYGALTLNGEEYIQFKQYAGKHVLFVSVATYuGLAAQYP 1

>GPX6_susScr Sus scrofa (pig) CU313307
0 MTPQFWASCLFSLCLVGFAQLIPKEQKM 0
0 MDCYKGVTGTIYEYGALTLNGEEYIPFKQYAGKHVLFVNVATYuGLTAQYP 1
2 ELNALQEELKPFGVVVLGFPCNQFGKQEPAKNSEILLGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPTSDLLGSSNQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWYHRASVSTVKSDIMEYLKQFKSE* 0

>GPX6_vicVic Vicugna vicugna (vicugna) frag
0 MDCYKGVTGTIYEYGALTLNGEEYIQFKQYSGKPVLFVNVATYuGLAAQYP 1

>GPX6_eriEur Erinaceus europaeus (hedgehog)
0 MIKQFWVSGLFPLFLAGLAQSTLEPQKMK 0
0 VDCYKEVTGTIFEYGALTLNGEEYIKFDQYAGKHVLFINVATYuGLTAQYI 1
2 ELNALQEELKPFNVVLLGFPCNQFGKQEPGKNSEILSGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGENEQKIFTFLK 0
0 HSCPHPSQLLGSLKNLSWEPVKVHDIRWNFEKFLVGPNGVPVMRWFHRAPVSTVKSDILEYLKQFN 0

>GPX6_sorAra Sorex araneus (shrew) frag
0 VDCYEGVTGTIYEYGALTLNSEEYIQFKQYAGKHVLFINVATFuGLTAQYI 1
2 ELNALQEELKPLGVVLLGFPCNQFGKQEPGKNSEILAGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKIFTFLK 0
0 NSCPPTSDVLGSPDHLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHQAPVSTVKSDILEYLKR

>GPX6_dasNov Dasypus novemcinctus (armadillo)
0 MIRHLHLFPLLLVGFVQPNPNEPTMK 0
0 TDCYKGVTGTIYEYGALTLNGEEYVQFKQYAGKHVLFVNVATYuGLTAQYP 1
2 ELNALQEELRPFGVVVLGFPCNQFGKQEPGKNSEILFGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLK 0
0 NSCPPPSELFGSSSQLFWEPLKVHDIRWNFEKFLVGPDGVPVMRWYHRASVSTVKSDMLAYLKQSKTK* 0

>GPX6_choHof Choloepus hoffmanni (sloth)
0 0
0 TDCYKGVTGTIYEYGALTLNGEEYIQFKQYAGKHVLFVNVATYuGLTAQYP 1
2 ELNALQEELKPFGVVVLGFPCNQFGKQEPGKNSEILPGLR 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKIFTFLK 0
0 NSCPPPSELLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWYHRAPVSTVKSDVLDYLKQ* 0

>GPX6_loxAfr Loxodonta africana (elephant)
0 MIRQVWASYLFSLLLVGFVQPSPNQEKMK 0
0 TDCYKGVTGNIYEYGALTLNGEEYIQFKQYVGKHVLFVNVATYuGLTAQYP 1
2 ELNALQEELKPFGLVVLGFPCNQFGKQEPGKNSEILSGLK 2
1 YVRPGGGFVPNFQLFEKGDVNGEKEQKIFTFLK 0
0 NSCPPPSEILGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWYHKASVSTVKSDILEYLKQFEIQ* 0

>GPX6_echTel Echinops telfairi (tenrec) frag
0  QTFWASYLFLLFLVGFVHLSLSQKKMK 0
0 tDCYKGVTSTTYESLTRNGEECFQLKWYTGKHVHGVGVATYuGLALQ 1
2 eLNVLQEQMKSFSLVVLAFACSRLEEQEPGRDS  2
1          PQFQLFEKGDVNGEKEQKVYTFLK 0
0 NSCPPPSEILGSSSQLFWEPLKVHDIRWNFEKFLVGPDGVPVMRWYHRAPVSAVRADILEYLKQFEDQ* 0

GPX1 glutathione peroxidase from 25 vertebrates

>GPX1_homSap Homo sapiens (human) chr3 2 exons glutathione peroxidase pseudo:chrX
0 MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA* 0

>GPX1_panTro Pan troglodytes (chimp)
0 MCAARLAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA* 0

>GPX1_macMul Macaca mulatta (rhesus)
0 MCAARLAAAAYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFQTIDIEPDIEALLSQGPSSA* 0

>GPX1_tupBel Tupaia belangeri (tree_shrew)
0 MCAARRAAAAQAVYTFSARPLGAEEPMSLGSLRGKVLLIENVASLuGTTVRDYTQMNDLQRRLGIRSLVVLGFPCNQFRHQ 0
0 KSKNEEILKSLKYVRIGDGFEPNFTVFEKCEVNGAKAHPLFAFLREALPAPSDDPCALMTDPKFITWSPVCRNDIAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLSQGP* 0

>GPX1_musMus Mus musculus (mouse)
0 MCAARLSAAAVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLuGTTIRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQ 0
0 ENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPTPSDDPTALMTDPKYIIWSPVCRNDIAWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIETLLSQQSGNS* 0

>GPX1_ratNor Rattus norvegicus (rat)
0 MSAARLSAVAVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTTRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQ 0
0 ENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPAPSDDPTALMTDPKYIIWSPVCRNDISWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIEALLSKQPSNP* 0

>GPX1_oryCun Oryctolagus cuniculus (rabbit)
0 MCAARMQSVYSFSAHPLAGGEPVNLGSLRGKVLLIENVASLuGTTVRDYTQMNELQERLGPRGLVVLGFPCNQFGH. 0
0 ENAKNEEILNSLKYVRPGGGFEPNFMLFQKCEVNGAKAHPLFAFLREALPAPSDDPTALMTDPKFITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIQALLSKGSGGA* 0

>GPX1_canFam Canis familiaris (dog)
0 MCAAALAAAAPSVYAFSARPLAGGEPMSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLRESLPAPSDDTTALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLSQGPSCA* 0

>GPX1_felCat Felis catus (cat)
0 MCAAPLAAAAPSVYSFSARPLAGGEPMSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLRQALPAPSDDATALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLSQGPSCA* 0

>GPX1_equCab Equus caballus (horse)
0 MCAAQLAAARSVYAFSARPLAGGEPLSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLREALPAPSDDATALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLTQGPSCA* 0

>GPX1_bosTau Bos taurus (cow)
0 MCAAQRSAAAPTVYAFSARPLAGGEPFNLSSLRGKVLLIENVASLuGTTVRDYTQMNDLQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNCLKYVRPGGGFEPNFMLFEKCEVNGEKAHPLFAFLREVLPTPSDDATALMTDPKFITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFLTIDIEPDIETLLSQGASA*  0

>GPX1_eriEur Erinaceus europaeus (hedgehog)
0 MCAAPAAAQSVHAFSARPLVGGEPISLGSLQGKVLLIENVASLuGTTVRDYTQMNELQQRFGPRGLVVLGFPCNQFGHQ 0
0 ENTKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAQAHPLFAFLREALPAPSDDATSLMTDPKLITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFPTIDIQPDIEALLAKQPLSS* 0

>GPX1_sorAra Sorex araneus (shrew)
0 MCAAPAAAAMAAQRSVHAFSARPLVGGEPISLGSLQGKVLLIENVASLuGTTVRDYTQMNELQQRFGPRGLVVLGFPCNQFGHQ 0
0 ENTKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAQAHPLFAFLREALPAPSDDATSLMTDPKLITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFPTIDIQPDIEALLAKQPLSS* 0 

>GPX1_dasNov Dasypus novemcinctus (armadillo)
0 MCAAALSAAQSVHAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENAKNEEILNCLKYVRPGGGFEPNFMLFEKCDVNGAQAHPLFAFLREALPAPSDDATALMTDPKFIIWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIESDIEALLSQGPSCA* 0

>GPX1_loxAfr Loxodonta africana (elephant)
0 MCAAPVAAALSVHAFSARPLAGGEPVNLGSLRGKVLLIENVASLuGTTVRDYTQMNDLQRRLGPRGLVVLGFPCNQFGHQ 0
0 ENGNKEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLREALPAPSDDATALMTDPKFIIWSPVCRNDISWNFEKFLVGPDGVPVRRYSRRFPTIDIEADIEALLSKGPSCA* 0

>GPX1_monDom Monodelphis domestica (opossum)
0 MCAARSVHAFSARLLGSGELLSLASLRGKVLLIENVASLuGTTIRDYTQMNELQKRHGGRGFMVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKFVRPGSGFEPNFMLFEKCEVNGEKAHPLFTFLREALPAPSDDTNSLMTDPKLIIWSPVCRNDISWNFEKFLVGPDGVPVKRYSRRFETINIEKDIEDLLTKMPKEV* 0

>GPX1_ornAna Ornithorhynchus anatinus (platypus)
0 MCASQSVYGFSGRLLRSGEPFSLAALRGKVLLIENVASLuGTTVRDYTQMNELQSLYGPRGLAVLGFPCNQFGHQ 0
0 ENAKNEEILNSLKYVRPGNGFEPNFTMFEKCEVNGEKAHPLFAFLRESLPTPSDDPTSLMNDPKFITWSPVCRNDISWNFEKFLVGPDGVPVKRYSRRFETINIKEDIEVLLGQMSSYFQ* 0

>GPX1_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01BQVNH 454
0                                 NGEKAHPLFAFLRESLPTPSDDPTSLMNDPKFIIWSPVCRNDISWNFEKFLVGPDGVPLRYSRRFETINIKEDIAMLLDQ

>GPX1_galGal Gallus gallus (chicken)
0 MAATGLAGILARPLGAAEPLALSSLRGKVLLVVNVASLuGTTTRDFLQLNELQQRYGPRGLRVLGFPCNQFGHQ 0
0 ENATNEEILRSLEYVRPGNGFKPNFTMFEKCEVNGKGAHPLFAFLREALPFPHDDPSALMTNPQYIIWSPVCRNDVSWNFEKFLVGPDGVPFRRYSRHFETIKLQDDIELLQ* 0

>GPX1_anoCar Anolis carolinensis (lizard) taa-stop
0 MAGLPVRGLAGLLARPLGAKDPVDLGSSRGRVVLVSNVASLuGTTARDFTQFSELQRRFGPRGFVVLAFPCNQFGHQ 0
0 ENATNEEILNSLKYVRPGNGYEPNFTVFEKCEVNGENAHPLFKFLKDELPYPHDDAVSLMANPQNIIWSPVCRNDISWNFEKFLIGPDGKPFKRYSRRFETIKIQDDIEKLQ* 0

>GPX1_xenTro Xenopus tropicalis (frog)
0 MRLAMVSRTVYEFSARLLSAGENTALSQYKGRVLLIENVASLuGTTIRDYTQMSRLQSMYGPRGLQVLAFPCNQFGHQ 0
0 ENSGNQEILNILKHVRPGGGFEPNFPLFEKVDVNGEKEHPLFTFLKGQLPYPSDDSISLMQDPKSIIWSPVRRNDIAWNFEKFLIARNGVPYKRYGRRFETFNIQQDIEKLLSLSSAP. 0

>GPX1b_danRer Danio rerio (zebrafish)
0 MAGIKSFYDITAKTLTGEEFKFSSLQGKVVLIENVASLuGTTSRDYTQMNELHERFAEKGLVVLGVPCNQFGYQ 0
0 ENCTNEEILLSLKYVRPGNGYEPKFQLLEKVDVNGKNAHPLFTFLKEKLPFPSDEPMPFMSDPKFIIWSPVCRNDIAWNFEKFLIGSDGVPFKRYSRRFLTSGIDGDIKKLLSIP* 0

>GPX1_tetNig Tetraodon nigroviridis (pufferfish)
0 MAANFYDLTAKLLTGEAFNFSSLQGKVVLIENVASLuGTTTRDYTQMNELHERYASKGLVVLGVPCNQFGHQ 0
0 ENCKNEEILLSLKHVRPGHGFEPKFQLLEKVDVNGKDAHPLFQFLREKLPSPSDDPSALISDPKLIIWSPVCRNDVAWNFEKFLVGPDGVPFKRYSRKFLTSSIESDIKKLLSQA* 0

>GPX1_takRub Takifugu rubripes (fugu)
0 METQFYDLTAKLLTGEAFNFSSLQGKVVLIENVASLuGTTSRDYTQMNELHERYAGQGLVILGVPCNQFGHQ 0
0 ENCKNEEILSSLKYVRPGNGFEPKFQLFEKVDVNGKDAHPLFQFLREKLPFPSDDPTALMSDPKLINWSPVCRNDVSWNFEKFLIGPDGVPFKRYSRKFLTSSIEGDIKKLLSQA* 0

>GPX1_gasAcu Gasterosteus  aculeatus (stickleback)
0 MARMAAKFYDLSAKLLTGETFNFSSLQGKVVNVASLuGTTSRDYTQMNELHERYAGKGLVILGVPCNQFGHQ 0
0 ENCKNEEILLSLKYVRPGNGFEPKFQLLEKVDVNGKDAHPLFVFLKGKLPYPSDEPGALMNDPKLILWSPVCRNDVSWNFEKFLIGSDGVPFKRYSRRFLTSDIEGDIKKLLSQA* 0

>GPX1_oryLap Oryzias latipes (medaka)
0 MASKFYSLTAKLLSGEIFNFSVLQGKVVLIENVASLuGTTTRDYTQMNELHERYASKGLVILGVPCNQFGHQ 0
0 ENCKNEEILLSLKHVRPGNGFEPKFQLLEKVDVNGKDAHPLFAFLREQLPTPSDDPAPLMTDPKFIIWSPVCRNDVAWNFEKFLVGPDGTPFKRYSRRFLTSDVEGDIKKLLSQT* 0

>GPX1_eptBur Eptatretus burgeri (hagfish) BJ648558 tga-sel tag-stop frag
0       SARGIAKSFYELSARNLAGuELVQFSKFRDKVVLIENVASLuGTTSRDFTQMNQLHQRLGVHGLVVLGFPCNQFGHQ 0
0 ENATNEELLQSLKYVRPGRGFEPNFPIFDKCEVNGANAHPLFTFLKEHLPLPSDNPTCFMSDCKSIIWSPVQRSDIAWNFEKFLIAPNGEPFRRYSKLYQTIDLEPDICKLLGL* 0

GPX2: 32 Vertebrate Sequences

>GPX2_homSap Homo sapiens (human) chr14 2 exons gastrointestinal glutathione peroxidase 2, U in cow pig rat chicken frog trout scorpion
0 MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_panTro Pan troglodytes (chimp)
0 MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_macMul Macaca mulatta (rhesus)
0 MAFIAKSFYGVSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPHDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_calJac Callithrix jacchus (marmoset)
0 MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 LQCGGIRQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_tarSyr Tarsius syrichta (tarsier)
0 MACIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQVSERQCRFPRRLVVLGFPCKQFGHQ 0
0 ...SRTVVDSYVRPGGGYQPTFTLLQKCDVNGQNEHPVFAYLKDKLPYPQDDPHSLMTDPKFIIWSPVRRSDVAWNFEKFLVGPEGEPFRRYSRTFPTISIEPDIKRLLKVAI* 0

>GPX2_otoGar Otolemur garnettii (galago)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLQYVRPGGGFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPLTLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_micMur Microcebus murinus (mouse_lemur)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKFVRPGGGYQPTFTLIQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFHTINIEPDIRRLLKVAI* 0

>GPX2_tupBel Tupaia belangeri (tree_shrew)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLQYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_musMus Mus musculus (mouse)
0 MAYIAKSFYDLSAIGLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYNQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI* 0

>GPX2_ratNor Rattus norvegicus (rat)
0 MAYIAKSFYDLSAIGLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFSLTQKCDVNGQNQHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI* 0

>GPX2_cavPor Cavia porcellus (guinea_pig)
0 MAYIAKSFYDLSAISLDGEKIDFNTFRGRAVLIENVASL&GTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ...AQCGGFSDVSWYFEKFLIGPEGEPFRRYSRTFHTINIEPDIKRLLKVAI* 0

>GPX2_speTri Spermophilus tridecemlineatus (squirrel)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNDLQCRFPRRLVVLGFPCNQFGHQ 0
0 0

>GPX2_ochPri Ochonta princeps (pika)
0 MAYIAKSFYDLTAISLDGEKVDFNTFRGRAVLIENVASLuGTTSRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLQYVRPGGGYQPTFTLVQKCEVNGQNQHPVFAYLKDKLPYPYDDPFSFMTDPRFILWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKLAI* 0

>GPX2_oryCun Oryctolagus cuniculus (rabbit)
0 MAYLAKSFYDLTAVSLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRYPRRLVVLGFPCNQFGHQ 0
0 ENCQDEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNQHPVFTYLKDKLPYPHDDPFSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_canFam Canis familiaris (dog)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPLSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_felCat Felis catus (cat)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_equCab Equus caballus (horse)
0 MAYIAKSFYDLSAISLDGEKIDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNQHPVFAYLKDKLPYPYDDPLSLMTDPKFIIWSPVCRSDVSWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0 

>GPX2_myoLuc Myotis lucifigus (microbat)
0 MAFIAKSFYDLSAISLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRYPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGDGFQPTFTLAQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKYIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI* 0

>GPX2_turTru Tursiops truncatus (dolphin)
0 MAYVAESFYDLSAISLDGEKVDFNTFRGMAVLIENVSSLuHNHRDFTQLNELQCRFPAPVILL... 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLIQKCDVNGQNEHPVFTYLKDKLPYPYDDPFSLMTDPKFIIWSPVHRSDVSWNFEKFLVGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_bosTau Bos taurus (cow)
0 MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLVQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI* 0

>GPX2_vicVic Vicugna vicugna (vicugna)
0 ...LDGEKVDFNTSRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLVQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_eriEur Erinaceus europaeus (hedgehog)
0 MAYIAKSFYDLSAISLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFTLAQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLLTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEADIKRLLKVAI* 0

>GPX2_sorAra Sorex araneus (shrew)
0 MGKKIDFNTFRGRVVLIENVASLuGTTTRDYTQLNELQARFPRRLVVLGFPCNQFGHQ 0
0 0

>GPX2_dasNov Dasypus novemcinctus (armadillo)
0 MGKKIDFNTFRGRVVLIENVASLuGTTTRDYTQLNELQARFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGNDFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKYIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_loxAfr Loxodonta africana (elephant)
0 MTHIAKSFYDLSAISLEGDKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCKNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPLSLMTDPKFIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI* 0

>GPX2_echTel Echinops telafair (hedgehog)
0 MAYIAKSFYDLSATSLDGDKIDFNAFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENSQNEEILNSLKYVRPGGGFQPTFTLAQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSFMTDPKFFIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFQTISIEPDIKRLLKVAI* 0

>GPX2_monDom Monodelphis domestica (opossum)
0 MAHIAKSFYDLSATSLEGERIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVILGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGAGFQPNFVLVQKCEVNGQNEHPVFAYLKDKLPYPDDDPFSLMTDPKHIIWSPVRRSDIAWNFEKFLVGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI* 0

>GPX2_ornAna Ornithorhynchus anatinus (platypus)
0 MARIAKSFYDLSATSLNGEKVDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQRLFPRRLVILGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGNGFEPHFVLIQKCEVNGQNEHPVFSYLKAKLPYPEDDPSALMTDPKLIIWSPVRRSDVAWNFEKFLVGPEGEPFRRYSRTFPTIDIEPDIKRLLKVAI* 0

>GPX2_galGal Gallus gallus (chicken) chicken cDNA missing genome
0 MSVPIAKSFYDLSATSLQGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQ 0
0 ENCQNEEILNSLKYVRPGGGFQPTFSLTQKCDVNGQNQHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI* 0

>GPX2_xenTro Xenopus tropicalis (frog) cDNA
0 MAYIAKSFYDLYATNIDGEKVDFNVFRGRVVLIENVASLuDTTVRDYTQLNELQTKYPRRLVVLGFPCNQFGYQ 0
0 ENCKNEEILNSLKYVRPGKGFVPGFTLFQKCDVNGKDTHSVFAYLKDKLPVPDNEPAALISDPRYIVWNPVHRSDISWNFEKFLIGPEGEPFKRYNKNFQTISIEPDIQRLLKLTKHLELCVCMYATDNYYMAAHKKI* 0

>GPX2_calMil Callorhinchus milii (elephantfish) tga-cys tag-stop full
0 MANSRKFYGFSTKLLNGQTLNFSKFKGKVVLIENVASLuGTTARDYTQMNELQSRYSREGFAVLGFPCNQFGFQ 0
0 ENCKNDEILKTLKYVRPGGGYEPNFTMFQKSIVNGDGTHPLFAYLKEKLPYPDDDPVSFMKDPQSINWSPVCRSDISWNFEKFLIGPDGEPFKRYSKKFETIQIEPDIQRLLKVAK* 0

>GPX2_petMar Petromyzon marinus (lamprey) cDNA LyEST1379 tga-sel tag-stop full
0 MKSFYELSAKTLGGELVSFSRYRGKVVLVENVASLuGTTTRDFTQLNELQGRYGAQGLAVLGFPCNQFGHQ 0
0 ENSQNEEILNTLKYVRPGSGYEPNFTMFAKCEVNGKDTHPVFEFLKEKLPLPSDDPISFLQDPKHIIWSPVSRSDIAWNFEKFLVGPDGQPFKRYSKKFQTINIEEDLKFLLKQVK* 0

GPX4: 3 vertebrate sequences

>GPX4_homSap Homo sapiens (human) at chr19:10549651057778 7 exons glutathione peroxidase 4
0 MSLGRLCRLLKPALLCGALAAPGLAGTM 0
0 CASRDDWRCARSMHEFSAKDIDGHMVNLDKYR 2
1 GFVCIVTNVASQuGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQ 0
0 EPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGN 2
1 AIKWNFTK 0
0 FLIDKNGCVVKRYGPMEEPL 0
0 VIEKDLPHYF* 0

>GPX4_ornAna Ornithorhynchus anatinus (platypus) uc002lrg.1 EG341043
0 MSLFRLARLAKPLLLGGAVAVPALRRTM 0
0 CASPDDWRCANSIYEFSAEDIDGKLVSLEKYR 2
1 GKVCIITNVASKuGKTEVNYTQLVDLHAQYAGQGLRILGFPCNQFGRQ 0
0 EPGTNSEIKEFAAGYNVKFDMFSKVCVNGDEAHPLWKWLKDQPKGKGTLGK 2

>GPX4_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01DM9S9 454
0   LFRLARFAKPLLLGGAVAVPALRRTM 0
0 CASPDDWRCANSIYDFSAEDIDGNSVSLEKYR 2
1 GKVCIITNVASKuGKTEVNYTQLVDLHAQYVEQGLRILGFPCNQFGKQ 0
0 EPGTNSEIKEFAAGYNVKFDMFSKVCVNGDEAHPLWKWLKDQPKGKGTLGN 2
1 AIKWNFTK 0
0 FLIDREGQVVKRYGPMDEPR 0
0 VIEKDLPCY 

>GPX4_eptBur Eptatretus burgeri (hagfish) BJ646422 tga-sel frag
0                          GTM 0
0 ASNGEWQTAKNMFEFSAMDIDGNNVSLEKYR 2
1 GHVSIVVNVASKuGKTTVNYTQLSAMHAKYADSHGLRILAFPCNQFGKQ 0
0 EPGTDAEIKAFAAGYDVHFDLFSKIMVNGDDAHPLWKWMKSQRYGHGTLGN 2
1 AIKWNFTK 0
0 FLIDKEGQVVKRYGPIDDPV 0
0 VI

GPX7: 3 mammalian sequences

>GPX7_homSap Homo sapiens (human) chr1:5243796152444640 3 exons 7 no genomic U
0 MVAATVAAAWLLLWAAACAQQEQDFYDFKAVNIRGKLVSLEKYRGS 0
0 VSLVVNVASEcGFTDQHYRALQQLQRDLGPHHFNVLAFPCNFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYL 1
2 QTSGKEPTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALVRKLILLKREDL* 0

>GPX7_ornAna Ornithorhynchus anatinus (platypus) LOC100090031 
0 MVAATVAAAWLLLWAAACAQQEQDFYDFKAVNIRGKLVSLEKYKGR 0
0 VSLVVNVASECGFTDQHYRALQQLQKDLGPSHFNVLAFPCNQFGQQEPDGNKEIESFVRKTYGVSFPMFSKIAVSGAGANAAFKFL 1
2 TESSGEEPTWNFWKYLVSPDGKVVNSWDSTVSVEEVRPQITALVRKLILLKREDL* 0

>GPX7_tacAcu Tachyglossus aculeatus (echidna) EUPZL4S02GXFL4
0                      QLQKDLGPSHFNVLAFPCNQFGQQEPDSNKEIESFVRKTYGVSFPMFSKIAVSG

ALL GPX: Not formatted or ordered the way we'd like, but thrown out in this format temporarily

>human_5
MTTQLRVVHLLPLLLACFVQTSPKQEKMKMDCHKDEKGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPELNALQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPGLKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK
>chimp_5
MTTQLRVVHLLPLLLACFVQTSPKQEKMKVMDCHKDEKGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPELNAPQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPALKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK
>Horse_5
MTAQLRASYLFLLLLAGFVQTENNAEKMKTDCYKDVKDTIYEYSAVTLNGKEHIQFNQYAGKPVLFVNVATYCGLTAQYPELNTLQEELKPFDLVVLGFPCNQFGKQEPGENSEILPGLKYVRPGGGYVPNFQLFEKGDVNGEKEQKVFTFLKHSCPHPSDVLGSLKHISWEPIKVHDIRWNFEKFLVGPDGVPVMRWFHKTPVSTVKSDILAYLKQFKIKKGG
>Cow_5
MTIQLRASCLFLFFLAGFVQTNSNLEKMDCYKDVKGTIYDYDAFTLNGKEHIQFKQYAGKHVLFVNVATYCGLTAQYPELNALQEELKPFGLVVLGFPCNQFGKQEPGENSEILPGLKYVRPGGGYVPNFQLFEKGDVNGETEQKVFTFLKQSCPHPSEIMGSIKHISWEPIMVRDIRWNFEKFLVGPDGIPVMRWFHRTPVSTVKTDILAYMKQFKTK
>Dog_5
MTAWLGASYVLPILLVSFVQTNAKPEKTKMDCYKDVKGTIYEYEALTLNGNERIQFKQYAGKHVLFVNVATYCGLTAQYPELNSLQEELKPLGLVVLGFPCNQFGKQEPGENSEILPGLKYVRPGRGYVPNFQLFEKGDVNGEKEQKVFTFLKLSCPHPSEVLGSFRHISWDPVKVHDIRWNFEKFLVGSDGVPVLRWFHRTPISTVKEDILVYLKQLKMK
>Rat_5
MAIQLRVFYLVPLLLASYVQTTPRLEKMKMDCYKDVKGTIYNYEALSLNGKERIPFKQYAGKHVLFVNVATYCGLTIQYPELNALQDDLKQFGLVILGFPCNQFGKQEPGDNTEILPGLKYVRPGKGFLPNFQLFAKGDVNGEKEQEIFTFLKRSCPHPSETVVTSKHTFWEPIKVHDIRWNFEKFLVGPNGVPVMRWFHQAPVSTVKSDILAYLNQFKTI
>mouse_5
MVTELRVFYLVPLLLASYVQTTPRPEKMKMDCYKDVKGTIYDYEALSLNGKEHIPFKQYAGKHVLFVNVATYCGLTIQYPELNALQEDLKPFGLVILGFPCNQFGKQEPGDNLEILPGLKYVRPGKGFLPNFQLFAKGDVNGENEQKIFTFLKRSCPHPSETVVMSKHTFWEPIKVHDIRWNFEKFLVGPDGIPVMRWFHQAPVSTVKSDIMAYLSHFKTI
>Opossum_3
MAGGTLQVSWLLPLVLAAFVQPIWGQEKMKTDCYPGITSTIYDYGALTIDGDEYIPFKQYAGKYILFVNVATYuGLTAQYLELNALQEDMKSSGLVILGFPCNQFGKQEPGQNSEILSALKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPPSELIGTSSRLFWEPMRVHDIRWNFEKFLVGPDGIPIMRWYHRATVSTVKADILEYLQKHSSLDMSA
>human_3
MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYuGLTGQYIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRRQAALGVKRK
>chimp_3
MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYuGLTGQYIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRRQAALGVKRK
>Monkey_3
MARLLQASCLLSLLLAGFLPQSRGQDKSKMDCHGGVSGTIYEYGALTIDGEEYIPFKQYIGKYVLFVNVASYuGLTGQYIELNALQEELAPFGLVLLGFPCNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPVMRWHHRTTISNVKMDILSYMRRQAALGVKRK
>Rat_3
MARILRASCLLSLLLAGFVPPGRGQEKSKTDCHGGMSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTDQYLELNALQEELGPFGLVILGFPCNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPIMRWYHRTTVSNVKMDILSYMRRQAALGARGK
>Dog_3
MARLLRASCLLSLLLAGFVPPSRGQEKSKTDCHGGVSGTIYEYGALTINGEEYIPFKQYAGKYILFVNVASYuGLTGQYLELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGSPGRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVSTVKMDILAYMRRQAALAIKGK
>mouse_3
MARILRASCLLSLLLAGFVPPGRGQEKSKTDCHGGMSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTDQYLELNALQEELGPFGLVILGFPSNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPVMRWYHRTTVSNVKMDILSYMRRQAALSARGK
>Cow_3
MARLFRASCLLSLLLAGFIPPSQGQEKSKTDCHAGVGGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTGQYVELNALQEELEPFGLVILGFPCNQFGKQEPGENSEILATLKYVRPGGGFTPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGSPDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVNSVKMDILTYMRRRAVWEAKGK
>Horse_3
MARLLRASCLLSLLLAGFVPPSRGQEKSKTDCHAGVSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASYuGLTGQYVELNALQEEFAPFGLVILGFPSNQFGKQEPGENSEIPLTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKMPVPPTSELLGSPDRLFWEPMKIHDIRWNFEKFLVGPDGKPIMRWYHRTTVNTVKMDILAYMRREAALAARGK
>human_6
MFQQFQASCLVLLFLVGFAQQTLKPQNRKVDCNKGVTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYuGLAAQYPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDILEYLKQFNTH
>chimp_6
MFRQFQASCLVLLFLVGFAQQILKPQNRKVDCNKGVTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVATYuGLAAQYPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPMKVHDICWNFEKFLVGPDGVPVMRWFHQAPVSTVKSDILEYLKQFNTH
>Monkey_6
MIRQFQACCLVLLFLVGFAQQTLKPQNRKVDCNKRVTGTIYEYGALTLNSEEYIQFKQFAGKHVLFVNVATYuGLAAQYPELNALQEELKNFGVVVLAFPCNQFGKQEPGTNSEILLGLKYVRPGSGFVPNFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHRAPVSTVKSDILEYLKQFNAH
>Dog_6
MVPQLWASALLPLLLAGLAQPTQNTQKMKVDCYNGVMGTIYEYGALTLNNEEYVQFKQYAGKHVLFVNVATYuGLTAQYPELNALQEELKSFGVVVLGFPCNQFGKQEPGKNSEILSGLKHVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSNQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHQAPVSTVKADILAYLKQLKTE
>Horse_6
MIRQLWASSLFPLFLVGFAQLTPESQKMKMDCYKGVTGTIYEYGALTLNGEEYIQFKQYAGKHVLFINVATYuGLTAQYPELNALQEELKPFGVVLLGFPCNQFGKQEPGKNSEILSGLKYVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSPKQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHRASVSTVKSDILEYLKQFTPE
>Cow_6
MILQLWASCLFPLFLVGLAQLTPKQQQMKVDCYKGVTGTIYEYGALTLNGEEYIQFKQYVGKHVLFVNVATYuGLTAQYPELNALQEELKPFGVVVLGFPCNQFGKQEPAKNSEILMGLKYVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFLKNACPPTSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWYHRASVSTVKSDMLEYLKQFKSE
>Galago_6
MIWQFWASCLFSLFLVGFTQQILKPEQMKVDCNKDVAGTIYEYGALTLDGEYIQFKQYAGKHVLFVNVATYuGLAVQYPELNALQEDLRDFGVIILGFPCNQFGKEEPGKSPEEILSGLKYVRPGGGFVPNFQLFEKGDVNGENEQKVFTFLKNSCPPTSDVLGSSKHLFWEPMKVHDIRWNFEKFLVGPNGVPVMRWYHRAPVSKVKSDIQEYLKQDT
>Cow_1
MCAAQRSAAALAAAAPRTVYAFSARPLAGGEPFNLSSLRGKVLLIENVASLuGTTVRDYTQMNDLQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNCLKYVRPGGGFEPNFMLFEKCEVNGEKAHPLFAFLREVLPTPSDDATALMTDPKFITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFLTIDIEPDIETLLSQGASA
>Dog_1
MCAAALAAAAVAAAPRSVYAFSARPLAGGEPMSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLRESLPAPSDDTTALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLSQGPSCA
>Horse_1
MCAAQLAAAARRSVYAFSARPLAGGEPLSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLREALPAPSDDATALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLTQGPSCA
>Armadil_1
MCAAALSAAALAAQRSVHAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNCLKYVRPGGGFEPNFMLFEKCDVNGAQAHPLFAFLREALPAPSDDATALMTDPKFIIWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIESDIEALLSQGPSCA
>Cat_1
MCAAPLAAAALAAAAPRSVYSFSARPLAGGEPMSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLRQALPAPSDDATALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLSQGPSCA
>human_1
MCAARLAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA
>Eleph_1
MCAAPVAAAALRSVHAFSARPLAGGEPVNLGSLRGKVLLIENVASLuGTTVRDYTQMNDLQRRLGPRGLVVLGFPCNQFGHQENGNKEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLREALPAPSDDATALMTDPKFIIWSPVCRNDISWNFEKFLVGPDGVPVRRYSRRFPTIDIEADIEALLSKGPSCA
>Monkey_1
MCAARLAAAAVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFQTIDIEPDIEALLSQGPSSA
>Opossum_1
MCAARSVHAFSARLLGSGELLSLASLRGKVLLIENVASLuGTTIRDYTQMNELQKRHGGRGFMVLGFPCNQFGHQENAKNEEILNSLKFVRPGSGFEPNFMLFEKCEVNGEKAHPLFTFLREALPAPSDDTNSLMTDPKLIIWSPVCRNDISWNFEKFLVGPDGVPVKRYSRRFETINIEKDIEDLLTKMPKEV
>mouse_1
MCAARLSAAAQSTVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLuGTTIRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPTPSDDPTALMTDPKYIIWSPVCRNDIAWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIETLLSQQSGNS
>Rabbit_1
MCAARMAAAAQSVYSFSAHPLAGGEPVNLGSLRGKVLLIENVASLuGTTVRDYTQMNELQERLGPRGLVVLGFPCNQFGHLENAKNEEILNSLKYVRPGGGFEPNFMLFQKCEVNGAKAHPLFAFLREALPAPSDDPTALMTDPKFITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIQALLSKGSGGA
>chimp_1
MCAARLAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA
>Rat_1
MSAARLSAVAQSTVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLuGTTTRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPAPSDDPTALMTDPKYIIWSPVCRNDISWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIEALLSKQPSNP
>Shrew_1
MCAAPAAAAMAAQRSVHAFSARPLVGGEPISLGSLQGKVLLIENVASLuGTTVRDYTQMNELQQRFGPRGLVVLGFPCNQFGHQENTKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAQAHPLFAFLREALPAPSDDATSLMTDPKLITWSPVCRNDVSWNFEKFLVGPDGVPVRRYSRRFPTIDIQPDIEALLAKQPLSS
>Cat_2
MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI
>human_2
MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI 
>chimp_2
MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI
>Monkey_2
MAFIAKSFYGVSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPHDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI
>Dog_2
MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPLSLMTDPKFIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI
>Cow_2
MAYIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGFQPTFTLVQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKFIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI
>mouse_2
MAYIAKSFYDLSAIGLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYNQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI
>Horse_2
MAYIAKSFYDLSAISLDGEKIDFNTFRGRAVLIENVASLuGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGFQPTFTLVQKCEVNGQNQHPVFAYLKDKLPYPYDDPLSLMTDPKFIIWSPVCRSDVSWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI
>Hedgehog_2
MAYIAKSFYDLSATSLDGDKIDFNAFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQENSQNEEILNSLKYVRPGGGFQPTFTLAQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSFMTDPKFFIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFQTISIEPDIKRLLKVAI
>Rat_2
MAYIAKSFYDLSAIGLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGFQPTFSLTQKCDVNGQNQHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI
>Bat_2
MAFIAKSFYDLSAISLDGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRYPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGDGFQPTFTLAQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKYIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI
>Opossum_2
MAHIAKSFYDLSATSLEGERIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVILGFPCNQFGHQENCQNEEILNSLKYVRPGAGFQPNFVLVQKCEVNGQNEHPVFAYLKDKLPYPDDDPFSLMTDPKHIIWSPVRRSDIAWNFEKFLVGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI
>Platypus_2
MARIAKSFYDLSATSLNGEKVDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQRLFPRRLVILGFPCNQFGHQENCQNEEILNSLKYVRPGNGFEPHFVLIQKCEVNGQNEHPVFSYLKAKLPYPEDDPSALMTDPKLIIWSPVRRSDVAWNFEKFLVGPEGEPFRRYSRTFPTIDIEPDIKRLLKVAI
>human_4
MSLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQuGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF
>Cow_4
MSFSRLYRLLKPALLCGALAAPGLASTMCASRDDWRCARSMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQuGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNAEIKEFAAGYNVKFDLFSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPCYL
>mouse_4
MSWGRLSRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQuGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVKFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL
>Opossum_4
MSFSRLYRLVKPALLCAALAAPGLSSSMCASRNDWRCARSMHDFSAKDIDGRLVSLDKYKGYVCIVTNVASQuGKTDVNYTQLVDLHARYAENGLRILAFPCNQFGRQEPGSNAEIREFAAGYNVKFDMYSKICVNGDDAHPLWKWMKIQPRGKGILGNAIKWNFTKFLIDKDGCVVKRYGPMEEPQVIEKDLPCYL
>Rat_4
MSWGRLSRLLKPALLCGALAVPGLAGTMCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQuGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL
>chimp_4
MSLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQuGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF
>Monkey_4
MNLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQuGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF
>fish_1a
MAGTMKKFYDLSAKLLSGDLLNFSSLKGKVVLIENVASLuGTTVRDYTQMNELHSRYADQGLVVLGAPCN
QFGHQENCKNEEILQSLKYVRPGNGFEPKFQILEKLEVNGENAHPLFAFLKEKLPQPSDDPVSLMGDPKF
IIWSPVCRNDISWNFEKFLIGPDGEPFKRYSRRFLTIDIDADIKELLKRTK
>fish_1b
MAGIKSFYDITAKTLTGEEFKFSSLQGKVVLIENVASLuGTTSRDYTQMNELHERFAEKGLVVLGVPCNQ 
FGYQENCTNEEILLSLKYVRPGNGYEPKFQLLEKVDVNGKNAHPLFTFLKEKLPFPSDEPMPFMSDPKFI 
IWSPVCRNDIAWNFEKFLIGSDGVPFKRYSRRFLTSGIDGDIKKLLSIPK
>frog_?
MVSRTVHEFSARLLTSGENTALSQYKGRVLLIENVASLuGTTIRDYTQMSKLQSTYGSRGLQVLAFPCNQFGHQENSNNQEILNLLKYVRPGGGFEPNFPLFEKVDVNGEKEHPLFTF
LKEQLPYPSDDSISLMQDPKSIIWSPVRRNDIAWNFEKFLITKNGVPYKRYGRRFETVNIQQDIEKLLHE
NCA
>frog_??
MVSRTVYEFSARLLANGENAALSQYRGRVLLIENVASLuGTTIRDYTQMSRLQDMYGSRGLQVLAFPCNQFGHQENSGNQEILNILQHVRPGGDFKPNFPLFEKVDVNGEKEHPLFTF
LKQQLPYPSDDSLSLMQDPKSIIWSPVRRNDIAWNFEKFLITKNGVPYKRYGRRFETFNIQQDIEKLLDE
KCA
>human_8
MEPLAAYPLKCSGPRAKVFAVLLSIVLCTVTLFLLQLKFLKPKINSFYAFEVKDAKGRTV
SLEKYKGKVSLVVNVASDCQLTDRNYLGLKELHKEFGPSHFSVLAFPCNQFGESEPRPSK
EVESFARKNYGVTFPIFHKIKILGSEGEPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKF
WRPEEPIEVIRPDIAALVRQVIIKKKEDL
>chimp_8
MEPLAAYPLKCSGPRAKVFAVLLSMVLCTVTLFLLQLKFLKPKINSFYAFEVKDAKGRTVSLEKYKGKVS
LVVNVASDCQLTDRNYLGLKELHKEFGPSHFSVLAFPCNQFGESEPRPSKEVESFARKNYGVTFPIFHKI
KILGSEGEPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKFWRPEEPIEVIRPDIAALVRQVIIKKKEDL
>monkey_8
MEPLAAYPLKCSGPRAKVFAVLLSMVLCTVTLFLLQLKFLKPKINSFYAFEVKDAKGRTVSLEKYKGKVS
LVVNVASDCQLTDRNYLALKELHKEFGPSHFSVLAFPCNQFGESEPRPSKEVESFARKNYGVTFPIFHKI
KILGSEGEPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKFWRPEEPIDVIRPDIAALVRKLIIKKKEDL
>cow_8
MEPLTAYPLRCSGPKAKAFAVLLSMVLCTVMLFLLQLKFLKPKINSFYTFEVKDANGRVVSLEKFKGKVA
LVVNVASDCQLTDRNYLALQELHKEFGPFHFSVLAFPCNQFGESEPRPSKEVVSFARNNFGVTFPIFHKI
KILGSEAEPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKTWRPEEPIEVIRPEIAALIRQMIIKKKEDL
>dog_8
MEPLTAYPLRCSGSKAKVFAVLLSMVLCTVMLFLLQLKFLKPKINSFYTFEVKDAKGRTVSLEKFKGKVT
LVVNVASDCQLTDRNYLALQELHKEFGPFHFSVLAFPCNQFGESEPRPSKEVVSFARNNYGATFPIFHKI
KILGSEADPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKSWRPEEPIEVIRPEIAALVRQMILRKKDDL
>mouse_8
MEPFAAYPLKCSGPKAKIFAVLLSMVLCTVMLFLLQLKFLKPRTNSFYSFEVKDAKGRTVSLEKFKGKAS
LVVNVASDCRFTDKSYQTLRELHKEFGPYHFNVLAFPCNQFGESEPKSSKEVESFARQNYGVTFPIFHKI
KILGPEAEPAFRFIVDSSKKEPRWNFWKYLVNPEGQVVKFWRPEEPLEAIRPHVSQMIGQIILKKKEDL
>opossum_8
MEPFAAPTPLKCSIPKARVIAVLLSMVLCSVTLFLLQLKYLKPRIKDFYSFEVKDSRGRTVSLEKYRGKA
SLIVNVATNCQHTDRNYLALQELHREFGSFHFNVLAFPCNQFGESEPGLSHEIEAFVKANYGVTFPIFHK
IKILGSEGEPAFKFLVDSSKKEPRWNFWKYLVNPEGKVVKYWRPEESLEAIRPDVAALVRQIIIKKKEDL
>chicken_8
MEPLTAAYPLKYSVPKARVFVVFLSMVFCTAVLCLLQLRFFKPKTKDFYSFEIKDSRGRIVSLEKYRGKA
TLVVNVASYCQHTDKNYIALQDLHREFGPSHFTVLAFPCNQFGESEPGSSQEIEAFAKGNYGVTFPVFHK
IKILGSEAEPAFKFLIDSSKKEPRWNFWKYLVSPEGKVVKFWRPEEPIESIKPEIASLIRQIIMKKREDL
>fish_8
MEALGGYPSKSSASRAGLFKVLLSVALCMGSLYLLQNKLSKSRKTKDFYSYEVKDARGRTVSLEKYRGK
ATLVVNVASGSELTEQSYRALQELHRELGTSHFNVLAFPCSQYGDTESGTSREIEAFA
KSNYGVTFPIFNKIKIMGSEAEPAFRFLTDSVQKIPKWNFWKFLVSPEGQVVRFWKPEEPVSDIRKEATTLVRNIILKKRQEL
>platypus_8
MEPLLSSPSKCSVAKARVLAVLLSLVLCTAILCLLQLRSIKPKIKTDFYSFQVKDSKGRTVALEKFRGKVSLIV
NVASNCQYTERNYLALQELHREFGPSHFSVLAFPCSQFGDSEPGPSQEIEAFAKGNYGATFPIFHKIKILGSEAEPAFRYLI
DSSKKEPRWNFWKYLVNPEGKVVKFWRPEEPFEIIKPDIAALVRQIIMKKKEDL
>human_7
MVAATVAAAWLLLWAAACAQQEQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQL
QRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTSGKEP
TWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALVRKLILLKREDL
>mouse_7
MVAAVATAWLLLWAAACAQSEQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQ
RDLGPHHFNVLAFPCNQFGQQEPDTNREIENFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTSGKEPT
WNFWKYLVDPDGKVVGAWDPTVPVAEIKPRITEQVMKLILRKREDL
>rat_7
MVAAVATAWLLLWAAACTQSEQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQ
RDLGPYHFNVLAFPCNQFGQQEPDSNREIENFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTSGKEPT
WNFWKYLVAPDGKVVGAWDPTVPVEEIKPRITEQVMKLILQKREDL
>chimp_7
MVAATVAAAWLLLWAAACAQQEQDFYDFKAVNIRGKLVSLEKYRGSVSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARR
TYSVSFPMFSKIAVTGTGAHPAFKYLARTSGKEPTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITAL
VRKLILLKREDL
>monkey_7
MVAAAAWLLLWAVACAQREQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAR
TSGKEPTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALVRKLILRKREDL
>Platypus_7
MPTPGGLALSLALTLALAGPGSEPRRAQREPDFYSFKVVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQKDLGPSHFNVLAFPCNQFGQQEPDGNKEIESFVRK
TYGVSFPMFSKIAVSGAGANAAFKFLTGSSGEEPTWNFWKYLVSPDGKVVNSWDSTVPVEEIKPHITALLRRMIMKKKDEL
>Opossum_7
MPSPLWCSPEAHCKARKHLARPDRTHKRHCIEADCKPVSLVVNVASECGYTDQHYRALQQLQRELGPYHF
NVLAFPCNQFGHQEPDTNKEIENFARKTYGVSFPMFSKIAVRGTGANAAFKYLTESSGEEPTWNFWKYLV
APNGKVVGTWDSTVPVEEIRPKIHELVGKLILGKKDEL
>Cow_7
MVAARAAAWLLLAAAACAPREQDFYDFKAVNIRGKLVSLEKYRGSVS
LVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTG
TSGKEPTWNFWKYLVAPDGKVIGAWDPTVSVEEIRPQITALVRKLILKKREDL
>Chick_7
MLLAITALLLLAFSATQQKETDFYTFKVVNIRGKLVSLEKYRGSVS
LVVNVASECGFTDSHYKALQQLQKDLGPYHFNVLAFPCNQFGQQEPDTNKEIESFARKTYGASFPMFSKVAVSGAGAIPAFKYL
DSTGEEPTWNFWKYLVDPNGKVVKAWDSTVSVEEIRPHVTELVRKIILKKKDEL
>fish_7
MGMFLRAFTLITLVCLLEAKQKDFYTFKVVNSRGRLVSLEKYRGSVS
LAVNVASECGYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQEPGSDKEIDSFVRRVYGVSFPIFSKIAVVGIGANNAYKYL
ASRKEPTWNFWKYLIDTDGKVVDAWGPEVSVKEIRPRITEMVRKLIIKRKEEL
>lizard?
MTPIAKSFYDLNATDLQGEKVDFNNFRGRVVLIENVASLuGTTVRDYTQMNLLQSKYPRRLVVLGFPCNQFGYQ
ENCTNEEILNSLKHVRPGSGFEPNFTLCQKCEVNGKNTHSVFAYLKDKLPYPDDNPSCL
MMEPKFILWSPVSRNDISWNFEKFLVGPEGEPFKRYSPRFLTTNIEPDIQRLLKLA
>lizard??
MMTPIAKSFYDLNATDLQGEKVDFNNFRGRVVLIENVASLuGTTVRDYTQMNLLQSKYPRRLVVLGFPCNQFGYQ
ENCTNEEILNSLKHVRPGSGFEPNFTLCQKCEVNGKNTHSVFAYLKDKLPYPDDNPSCL
MMEPKFILWSPVSRNDISWNFEKFLVGPEGEPFKRYSPRFLTTNIEPDIQRLLKLAK
>lizard???
WRGRWARRTPWTWAPSRGRVVLVSNVASLuGTTARDFTQFSELQRRFGPRGFVVLAFPCNQFGHQ
ENATNEEILNSLKYVRPGNGYEPNFTVFEKCEVNGENAHPLFKFLKDELPYPHDDAVSL
MANPQNIIWSPVCRNDISWNFEKFLIGPDGKPFKRYSRRFETIKIQDDIEKLLQ
>platypus_1
MCASPNSVYGFSGRLLRSGEPFSLAALRGKVLLIENVASLuGTTVRDYTQMNELQSLYGPRGLAVLGFPCNQFGH
QENAKNEEILNSLKYVRPGNGFEPNFTMFEKCEVNGEKAHPLFAFLRESLPTPSDDPTSL
MNDPKFITWSPVCRNDISWNFEKFLVGPDGVPVKRYSRRFETINIKEDIEVLLGQMSRE
>mouse_6
MAQKLWGSCLFSLFMAALAQETLNPQKSKVDCNKGVTGTVYEYGANTIDGGGFVNFQQYAGKHILFVNVA
SFCGLTATYPELNTLQEELKPFNVTVLGFPCNQFGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFEKGDV
NGDNEQKVFSFLKNSCPPTSELFGSPEHLFWDPMKIHDIRWNFEKFLVGPDGVPVMRWFHHTPVRIVQSD
IMEYLNQTSTQ
>armadil_2
MCAAALSAAALAAQRSVHAFSARPLAGGEPVSLGSLRGKVLLIENVASLu
GTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNCLKYVRPGGGFEPNFMLFEKCDVNGAQAHPLFAFLREALPAPSDDATAL
MTDPKFIIWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIESDIEALLSQGPSCA
>fish_3
ACNSAAGDSFHNYGAKTINGTQFIPFSHYAGKHVLVVNVATYuGLTFQYVELNALHEELRHLGFTILGFPCDQFGKQEPGENNEILSALKYVRPGNGFVPNFQLFEKGDVNGDGEQALFTFLKNACPPVGESFGATSNRLFWEPLKVNDIKWNFEKFLLDPDGRPVMRWFPRVNVSEVRADILKYFHQLLQTAQ
>chicken_2
MSVPIAKSFYDLSATSLQGEKIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVVLGFPCNQFGHQ
ENCQNEEILNSLKYVRPGGGFQPTFSLTQKCDVNGQNQHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRTFQTINIEPDIKRLLKVAI
>elFish_2
MANSRKFYGFSTKLLNGQTLNFSKFKGKVVLIENVASLuGTTARDYTQMNELQSRYSREGFAVLGFPCNQFGFQ
ENCKNDEILKTLKYVRPGGGYEPNFTMFQKSIVNGDGTHPLFAYLKEKLPYPDDDPVSFMKDPQSINWSPVCRSDISWNFEKFLIGPDGEPFKRYSKKFETIQIEPDIQRLLKVAK
>lamprey_2
MKSFYELSAKTLGGELVSFSRYRGKVVLVENVASLuGTTTRDFTQLNELQGRYGAQGLAVLGFPCNQFGHQ
ENSQNEEILNTLKYVRPGSGYEPNFTMFAKCEVNGKDTHPVFEFLKEKLPLPSDDPISFLQDPKHIIWSPVSRSDIAWNFEKFLVGPDGQPFKRYSKKFQTINIEEDLKFLLKQVK
>fish_2
MTFIAKTFYDLHATTLEGDTIDFNIYRGRVVLIENVASLuGTTTQDYTQLNELQSRYPHRLVVLGFPCNQFGYQENCSDGEILNSLKYVRPGEGYKPSFTIFQKCVVNGSDAHPVFSYLKDKLPYPDDDPVTLIQDPKYLVWNPVSRNDISWNFEKFLIGPEGEPFKRYSKKFQTINIEPDIQRLLKLTKN
>platypus_2
MARIAKSFYDLSATSLNGEKVDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQRLFPRRLVILGFPCNQFGHQENCQNEEILNSLKYVRPGNGFEPHFVLIQKCEVNGQNEHPVFSYLKAKLPYPEDDPSALMTDPKLIIWSPVRRSDVAWNFEKFLVGPEGEPFRRYSRTFPTIDIEPDIKRLLKVAI
>opossum_2
MAHIAKSFYDLSATSLEGERIDFNTFRGRAVLIENVASLuGTTTRDYTQLNELQCRFPRRLVILGFPCNQFGHQENCQNEEILNSLKYVRPGAGFQPNFVLVQKCEVNGQNEHPVFAYLKDKLPYPDDDPFSLMTDPKHIIWSPVRRSDIAWNFEKFLVGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI
>frog_2
MAYIAKSFYDLYATNIDGEKVDFNVFRGRVVLIENVASLuDTTVRDYTQLNELQTKYPRRLVVLGFPCNQFGYQENCKNEEILNSLKYVRPGKGFVPGFTLFQKCDVNGKDTHSVFAYLKDKLPVPDNEPAALISDPRYIVWNPVHRSDISWNFEKFLIGPEGEPFKRYNKNFQTISIEPDIQRLLKLTKHLELCVCMYATDNYYMAAHKKI

Basal selenoproteins: platypus (21), echidna (16), elephantshark (9), lamprey (11) and hagfish (7)

Outgroups are exceedingly important in determining ancestral characters along the evolutionary stem leading to placental mammals. The most important species are hagfish, lamprey, coelocanth/lungfish, and platypus/echidna. However only a fraction of the likely selenoprotein repertoire is available because of incomplete data.

These sequencs are current as of 6 May 2008. They derive from cdna at GenBank (both hagfish and lamprey selenoproteins were surprisingly well represented), at NCBI WGS contigs for cartilaginous fish and at the UCSC lamprey genome browser. Hagfish has no genome project underway so its transcripts have been intronated by homology. Platypus has both a genome assembly and a 454 transcrip project; echidna has only the latter. One interesting finding -- in addition to startling differences in degree of conservation of different selenoprotein -- is that SEPHS1 from both hagfish and lamprey have (ancestral) selenocysteine unlike mammals which have cysteine.

Summary of availablity by species:

>DIO1_ornAna Ornithorhynchus anatinus (platypus)
>DIO2_ornAna Ornithorhynchus anatinus (platypus)
>DIO3_ornAna Ornithorhynchus anatinus (platypus) 
>GPX4_ornAna Ornithorhynchus anatinus (platypus)
>GPX7_ornAna Ornithorhynchus anatinus (platypus)
>MSRB1_ornAna Ornithorhynchus anatinus (platypus)
>MSRB1_ornAna Ornithorhynchus anatinus (platypus)
>MSRB3_ornAna Ornithorhynchus anatinus (platypus)
>SELH_ornAna Ornithorhynchus anatinus (platypus)
>SELI_ornAna Ornithorhynchus anatinus (platypus)
>SELK_ornAna Ornithorhynchus anatinus (platypus)
>SELM_ornAna Ornithorhynchus anatinus (platypus)
>SELO_ornAna Ornithorhynchus anatinus (platypus)
>SELS__ornAna Ornithorhynchus anatinus (platypus)
>SELT_ornAna Ornithorhynchus anatinus (platypus)
>SELU1_oan data Ornithorhynchus anatinus (platypus)
>SEP15_ornAna Ornithorhynchus anatinus (platypus)
>SEPHS1_ornAna Ornithorhynchus anatinus (platypus)
>SEPHS2_ornAna Ornithorhynchus anatinus (platypus)
>SEPP1_ornAna Ornithorhynchus anatinus (platypus)
>SEPP2_ornAna Ornithorhynchus anatinus (platypus)  
>SEPW1_ornAna Ornithorhynchus anatinus (platypus) 

>DIO2_tacAcu Tachyglossus aculeatus (echidna)
>GPX1_tacAcu Tachyglossus aculeatus (echidna)
>GPX4_tacAcu Tachyglossus aculeatus (echidna)
>GPX5_tacAcu Tachyglossus aculeatus (echidna)
>GPX7_tacAcu Tachyglossus aculeatus (echidna)
>MSRB1_tacAcu Tachyglossus aculeatus (echidna)
>SELH_tacAcu Tachyglossus aculeatus (echidna) 
>SELK_tacAcu Tachyglossus aculeatus (echidna)
>SELM__tacAcu Tachyglossus aculeatus (echidna) 
>SELO_tacAcu Tachyglossus aculeatus (echidna) 
>SELS_tacAcu Tachyglossus aculeatus (echidna) 
>SELT_tacAcu Tachyglossus aculeatus (echidna)
>SELT_tacAcu Tachyglossus aculeatus (echidna)
>SELU1_tacAcu Tachyglossus aculeatus (echidna)
>SEP15_tacAcu Tachyglossus aculeatus (echidna)
>SEPHS1_tacAcu Tachyglossus aculeatus (echidna)
>SEPHS2_tacAcu Tachyglossus aculeatus (echidna)
>SEPP1_tacAcu Tachyglossus aculeatus (echidna)

>DIO1_calMil Callorhinchus milii (elephantfish)
>DIO2_calMil Callorhinchus milii (elephantfish)  
>DIO3_calMil Callorhinchus milii (elephantfish)
>GPX2_calMil Callorhinchus milii (elephantfish)
>SELO_calMil Callorhinchus milii (elephantfish)
>SELU1_calMil Callorhinchus milii (elephantfish) frag
>SEPHS1_calMil Callorhinchus milii (elephantfish) frag
>SEPP2_calMil Callorhinchus milii (elephantfish)
>SEPW2_calMil Callorhinchus milii (elephantfish)

>DIO2a_petMar Petromyzon marinus (lamprey) frag
>DIO2b_petMar Petromyzon marinus (lamprey) frag
>GPX2_petMar Petromyzon marinus (lamprey)
>MSRB3_petMar Petromyzon marinus (lamprey)
>SELO_petMar Petromyzon marinus (lamprey) frag
>SELS_petMar Petromyzon marinus (lamprey)
>SELT_petMar Petromyzon marinus (lamprey)
>SELU3_petMar Petromyzon marinus (lamprey)
>SELW_petMar Petromyzon marinus (lamprey)
>SEP15_petMar Petromyzon marinus (lamprey)
>SEPHS1_petMar Petromyzon marinus (lamprey)
>SEPN1_petMar Petromyzon marinus (lamprey)

>GPX1_eptBur Eptatretus burgeri (hagfish)
>GPX4_eptBur Eptatretus burgeri (hagfish)
>SELT_eptBur Eptatretus burgeri (hagfish)
>SELT_eptBur Eptatretus burgeri (hagfish)
>SELW_eptBur Eptatretus burgeri (hagfish)
>SEP15_eptBur Eptatretus burgeri (hagfish)
>SEPHS1_eptBur Eptatretus burgeri (hagfish)

GPX sites in lamprey browser needing evaluation:
GPX	Contig4536:10087-19433
GPX	Contig4536:13614-13712
GPX	Contig4536:17923-18132
GPX	Contig33644:113-3872
GPX	Contig41170:1909-3454
GPX	Contig3803:603-6660
GPX	Contig3036:20426-22172

These fragmentary sequences establish that echidna has the indicated gene but not whether selenocysteine is present.

>MSRB1_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01EKBYH 454
FCSFFGGEVFQNHFETGIYVCARCGYELFSSRSKFEHSSPWPAFTETIHPDSVAKREEPGRPGAFKVSCGKCGNGL

>SELH_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01B810Z 454
RALSLAAPHLPILLNPRQPRRNSFEVTLFGPDGTRTELWSGIKKGPPRRLKFPEPEMLADLLRSSLA

>SELK_tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01DCGU7 454
VMVYISNGHVLNGQNRSPWSLSYIKDFFWGILDFIIMFFKSMIHPNVKRGCRNSSSDSKYDDGRGPPGYPRRGMGRINHSNGPNPPPMAGG

>SELM__tacAcu Tachyglossus aculeatus (echidna) EUGXWLM01AWAS8 454
LNFRYEELERIPLSHMTRAEINQLVQDLGFYRKADRDAPVPPEFQQAPAK

>SELT_tacAcu Tachyglossus aculeatus (echidna) EUEMSW402BA71P 454
VFFLSNMIENQCMSTGAFEITLNDVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS

>SELT_tacAcu Tachyglossus aculeatus (echidna)  EUGXWLM01DXGYB 454
YRHIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVYACMMVFLLSNMIENQCMSTVAFEITLNDVPVWSKLESGHLPSMQQLVQILDNEMKLLNVHMDSIPHHRS

>SEPHS2 Tachyglossus aculeatus (echidna) EUPZL4S02I1W5B
QRIRRGKLVVSREDVELAYQEAMFSMAMLNRTAAGLMHTFNAHAATDI

>SEPP1_tacAcu Tachyglossus aculeatus (echidna) EUEMSW408ERBZB 454
GELERHAPPGVPVYAQDGPDPDVWSILGGGKDDFLVYDRCGRLTFHIRLPFSFLHFPYVEAAVLFSHRHDFCGNCSYY