Marsupial phyloSNPs

Introduction to Marsupial phyloSNPs

In this project, new genomic data from the Tasmanian devil (Sarcophilus harrisii), Tasmanian tiger (Thylacinus cynocephalus), and echidna (Tachyglossus aculeatus) are analyzed for significant changes at the protein coding level. The goal is to find single amino acid changes in one of these species at a highly invariant residue in a well-conserved exon in a gene with known or predictable tertiary structure. Such changes are thought to enrich for genetic changes with significant, adaptive biochemical or phenotypic consequences (1,2,3,4), in contrast to ordinary SNPs at positions of low conservation. Thus phyloSNPs are informative to the distinctive biology of the species carrying them and suggest a focus for subsequent experiment.

It is also of particular interest to determine the levels of variation within the Tasmanian devil population as a whole because the number of individuals have become low and possibly inbreed with adverse sequelae. For this it will be necessary to first determine sites of variation and then to genotype them across a large number of individuals.

Marsupial genomic and cDNA data to date has been quite limited compared to placental mammal. Yet as outgroup, metatheran animals provide important context to placentals and represent important context in understanding human protein evolution. The monotheres are inevitably limited by the paucity of extant species (basically platypus and echidna) and dim prospects for fossil DNA. Consequently echidna provides an important adjunct to the existing but incomplete platypus assembly. While extant birds and reptiles -- the preceding divergence node -- are abundant it must be remembered that a very considerable time elapsed (from 310 mry to 175 mry) prior to divergence of mammals with living representatives. This gap of 135 myr is comparable to the whole evolutionary record of theran mammals.

Assumed vertebrate phylogenetic tree

Marsupial relationships are taken from a 2009 paper establishing the mitochondrial genome sequences of the Tasmanian tiger (Thylacinus cynocephalus) and numbat (Myrmecobius fasciatus).

Newick tree that generates a marsupial-centric vertebrate phylogenetic tree:

((((((((((((sarHar,smiCra),myrFas),thyCyn),(macEug,triVul)),monDom),
((((loxAfr,proCap),echTel),(dasNov,choHof)),
((((((bosTau,turTru),susScr),vicPac),((equCab,(felCat,canFam)),(myoLuc,pteVam))),(eriEur,sorAra)),
(((((((((homSap,panTro),gorGor),ponPyg),macMul),calJac),tarSyr),(micMur,otoGar)),tupBel),
(((((musMus,ratNor),dipOrd),cavPor),speTri),(oryCun,ochPri)))))),
(ornAna,tacAcu)),
((galGal,taeGut),anoCar)),
xenTro),
(((tetNig,takRub),(gasAcu,oryLap)),danRer)),
calMil),
petMar);

Newick tree that generates the homo-centric vertebrate phylogenetic tree:

((((((((((((((((((homSap,panTro),gorGor),ponPyg),macMul),calJac),tarSyr),(micMur,otoGar)),tupBel),
(((((musMus,ratNor),dipOrd),cavPor),speTri),(oryCun,ochPri))),
(((((vicPac,susScr),turTru),bosTau),((equCab,(felCat,canFam)),(myoLuc,pteVam))),(eriEur,sorAra))),
(((loxAfr,proCap),echTel),(dasNov,choHof))),
(monDom,((macEug,triVul),(sarHar,thyCyn)))),
(ornAna,tacAcu)),
((galGal,taeGut),anoCar)),
xenTro),
(((tetNig,takRub),(gasAcu,oryLap)),danRer)),
calMil),
petMar);

Phylo-sorting data

This tab-delimited table enables four different sort orders. These are needed because data can be missing from species in a manner that varies by gene, making data alignment difficult. Some alignment tools also lose input order, so that needs to be recovered. The ordering here flattens the phylogenetic tree by taking human (arbitrarily) at the top and resolving ambiguous situations (eg mouse, rat) by putting species with the best assemblies first.

The first two columns provide sort order number for the 44 species alignment at UCSC as phylogenetic and alphabetic order respectively. The third and fourth columns do this for a larger set of 53 species for which data is commonly available (notably in marsupials). The fifth column supplies the genSpp acronym and the sixth the Newick tree format syntax. These two columns by themselves will correctly draw the vertebrate phylogenetic tree in all online software without further editing. The final columns provide genus, species, and common name.

..	..	..	..	......	((((((((((((			
46	10	54	10	anoCar	)),	Anolis	carolinensis	(lizard)
29	11	22	11	bosTau	,	Bos	taurus	(cow)
15	12	38	12	calJac	),	Callithrix	jacchus	(marmoset)
62	54	61	13	calMil	),	Callorhinchus	milii	(elephantfish)
32	13	28	14	canFam	)),(	Canis	familiaris	(dog)
23	14	46	15	cavPor	),	Cavia	porcellus	(guinea_pig)
41	15	21	16	choHof	)),((((((	Choloepus	hoffmanni	(sloth)
52	16	60	17	danRer	)),	Danio	rerio	(zebrafish)
40	17	20	18	dasNov	,	Dasypus	novemcinctus	(armadillo)
22	18	45	19	dipOrd	),	Dipodomys	ordii	(kangaroo_rat)
39	19	19	20	echTel	),(	Echinops	telfairi	(tenrec)
30	20	26	21	equCab	,(	Equus	caballus	(horse)
35	21	31	22	eriEur	,	Erinaceus	europaeus	(hedgehog)
31	22	27	23	felCat	,	Felis	catus	(cat)
44	23	52	24	galGal	,	Gallus	gallus	(chicken)
50	24	58	25	gasAcu	,	Gasterosteus	aculeatus	(stickleback)
12	25	35	26	gorGor	),	Gorilla	gorilla	(gorilla)
10	26	33	27	homSap	,	Homo	sapiens	(human)
37	27	17	28	loxAfr	,	Loxodonta	africana	(elephant)
58	56	14	29	macEug	,	Macropus	eugenii	(wallaby)
14	28	37	30	macMul	),	Macaca	mulatta	(rhesus)
17	29	40	31	micMur	,	Microcebus	murinus	(mouse_lemur)
42	30	16	32	monDom	),((((	Monodelphis	domestica	(opossum)
20	31	43	33	musMus	,	Mus	musculus	(mouse)
33	32	29	34	myoLuc	,	Myotis	lucifugus	(microbat)
56	57	12	35	myrFas	),	Myrmecobius	fasciatus	(numbat)
26	33	49	36	ochPri	)))))),(	Ochotona	princeps	(pika)
43	34	50	37	ornAna	,	Ornithorhynchus	anatinus	(platypus)
25	35	48	38	oryCun	,	Oryctolagus	cuniculus	(rabbit)
51	36	59	39	oryLap	)),	Oryzias	latipes	(medaka)
18	37	41	40	otoGar	)),	Otolemur	garnettii	(bushbaby)
11	38	34	41	panTro	),	Pan	troglodytes	(chimp)
53	39	62	42	petMar	)	Petromyzon	marinus	(lamprey)
13	40	36	43	ponPyg	),	Pongo	pygmaeus	(orang)
38	41	18	44	proCap	),	Procavia	capensis	(hyrax)
34	42	30	45	pteVam	))),(	Pteropus	vampyrus	(macrobat)
21	43	44	46	ratNor	),	Rattus	norvegicus	(rat)
54	58	10	47	sarHar	,	Sarcophilus	harrisii	(tasmanian_devil)
55	59	11	48	smiCra	),	Sminthopsis	crassicaudata	(dunnart)
36	44	32	49	sorAra	)),(((((((((	Sorex	araneus	(shrew)
24	45	47	50	speTri	),(	Spermophilus	tridecemlineatus	(squirrel)
60	60	24	51	susScr	),	Sus	scrofa	(pig)
61	61	51	52	tacAcu	)),((	Tachyglossus	aculeatus	(echidna)
45	46	53	53	taeGut	),	Taeniopygia	guttata	(finch)
49	47	57	54	takRub	),(	Takifugu	rubripes	(fugu)
16	48	39	55	tarSyr	),(	Tarsius	syrichta	(tarsier)
48	49	56	56	tetNig	,	Tetraodon	nigroviridis	(pufferfish)
57	62	13	57	thyCyn	),(	Thylacinus	cynocephalus	(tasmanian_tiger)
59	63	15	58	triVul	)),	Trichosurus	vulpecula	(bushytail_possum)
19	50	42	59	tupBel	),(((((	Tupaia	belangeri	(tree_shrew)
28	51	23	60	turTru	),	Tursiops	truncatus	(dolphin)
27	52	25	61	vicPac	),((	Vicugna	pacos	(lama)
47	53	55	62	xenTro	),(((	Xenopus	tropicalis	(frog)
								
44	44	53	53	genSpp	tree_syntax	genus	species	common
ph	al	ph	al

Candidate analysis

The first issue is error within the primary reads themselves; the second is whether the default 454 Newbler assembler correctly identified overelapping reads and put them together properly to give exon-spanning reads. Those issues are discussed elsewhere -- here it is assumed the reads at the PSU blast site are correct, so the entire focus is on subsequent bioinformatics. In some cases that results in retrospective identification and correction of errors, notably introduced frameshifts that are far too common.

After thorough evaluation, candidates are given a final heuristic score based on awarding 0,1,or 2 points for the following 13 critera:

• the change is real: multiple reads support each of the two amino acid values

• quality coverage: the entire exon can be recovered from multiple reads without manual frameshift correction

• processed pseudogenes can be recognized by reads long enough at flanks to identify neighoring exons now adjacent (resp GT-AG splice donors)

• non-processed pseudogenes can be distinguished by recovery of additional exons of the gene with expected levels of conservation

• paralogs and internal repeats are readily distinguishable from the exon under stead

• phylogenetic depth: multiple marsupials, monotremes, all placental branches, fish, chondrichthyes, possibly lamprey available

• homoplasy: the reduced alphabet consists of a single amino acid with the exception of Sarcophilus

• appropriate character of the change in amino acid properties

• amenability to accurate rapid scoring in many individual animals

• interpretability of structural significance of change within 3D structure or characterized domain

• interpretability of functional role of overall gene and of region containing the amino acid change

• previous relevant publications, animal kockout models, known human ortholog disease SNPs

• plausible relevancy of the change to cancer or facial tumor

When scoring is finished, the dummy table below will be filled in with real data and genes will become sorted by highest overall score (or by preferred columns appropriate to specialized purposes).

.....		valid	cover	psgen	paral	depth	alpha	AAcha	popul	struc	funct	pubmd	tumor	
ERN2  		1	1	1	1	1	1	1	1	1	1	1	1	12
MGAT5 		1	1	1	1	1	1	1	1	1	1	1	1	12
ACTL6B		1	1	1	1	1	1	1	1	1	1	1	1	12
IPO7  		1	1	1	1	1	1	1	1	1	1	1	1	12
PPFIA3		1	1	1	1	1	1	1	1	1	1	1	1	12
WDFY3 		1	1	1	1	1	1	1	1	1	1	1	1	12
XYLT1 		1	1	1	1	1	1	1	1	1	1	1	1	12
ATP4A 		1	1	1	1	1	1	1	1	1	1	1	1	12
VPS72 		1	1	1	1	1	1	1	1	1	1	1	1	12
ABCC1 		1	1	1	1	1	1	1	1	1	1	1	1	12
ACOT12		1	1	1	1	1	1	1	1	1	1	1	1	12
FLI1  		1	1	1	1	1	1	1	1	1	1	1	1	12
SPON1 		1	1	1	1	1	1	1	1	1	1	1	1	12
.....		13	13	13	13	13	13	13	13	13	13	13	13	.....

Case of ERN2

chr6_5971 ERN2 4
contig00001  length=355   numreads=5
KLPFTIPELVHASPCRSSDGVLYT
.....................F..
               ^        
15      R=3(75) H=2(50

Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5
and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in
tasmanian  devil (in this case, both individuals differ from Monodelphis by L->F), then differences between the two tasmanian devils
(here one individual has R at position 15, the other has H), and finally the number of experimental reads that confirm the nucleotide
difference and the sum of the quality scores. The sequences were assembled by Newbler (the official 454 assembler) which uses 
lower-case letters for less confident calls.

Pseudogene issues: ERN2 has not generated potentially confusing recent processed pseudogenes in mammals (lack of human, opossum or platypus genome Blat matches to ERN2 query). The variation observed here between individual tasmanian devils is implausibly an early stage in the loss of parent gene because of ERN2 functional essentiality; the exon cannot come from a decaying segmental duplication because coverage is high enough to also detect the main gene.

Paralog issues: The GeneSorter tool at UCSC shows a single significant full-length paralog in human, ERN1, also with 22 coding exons. The genes reside on different chromosomes but in regions with local homology of synteny. However this particular exon is a good match (3 differences out of 23), so there is potential for experimental difficulties in distinguishing them in short reads (including the following exon readily resolves them bioinformatically). In any event, at positions 15 and 20, ERN1 is identical at the amino acid level to ERN2. The gene duplication appears to have occured subsequent to amphioxus divergence earlier diverging metazoans are single-copy.

Homoplasy (recurrent mutation) issues: This exon is very conserved and does not exhibit repetitive sequence, compositional simplicity, or indels in any species in either paralog that could foster experimental error or alignment ambiguity. At position 15, the ancestral value is arginine in both paralogs. The G--> A transition to histidine in one individual is conservative under most circumstances (still basic) and arises from an arginine codon CpG hotspot conserved back to lamprey in 30 of 32 species with available data, yet histidine is not observed part of a reduced alphabet (ie R/H) at this position over many billions of years of branch length. Consequently R-->H is a significant change in this individual tasmanian devil.

Known variations: No human disease variants have been reported for either ERN2 or ERN1, probably attributable to essentiality. Site-specific mutation close to the exon here have been generated for K121P, D123P, W125A, and Q105E but only for ERN1. Naturally occuring coding SNPs in the human population relevent to the ERN2 exon are not known but low frequency alleles could emerge from the 1000 Genomes Project.

Side issues: a very ancient conserved leucine at position 21 appears to be transitioning to phenylalanine at marsupial node but has not been fixed, so settles out as L or F depending on lineage-sorting on each terminal marsupial leaf whereas placentals are all changed to phenylalanine (a phyloSNP caught in mid-air). While L and F might seem about the 'same' as amino acids, the branch length conservation totals say both are important but for different reasons: this is not a waffle codon nor reduced alphabet situation. This raises the question -- given the extreme conservation of this exon otherwise -- of whether the L-->F change at position 21 in both individuals has 'enabled' (made neutral or adaptive) an otherwise unfavorable R-->H change at position 15 in one individual.

Structural significance: By good fortune, the crystal structure of ERN1 (alternately called IRE1) has been published. The PDB 2HZ6 structure has good coverage of this particular exon. Consequently the marsupial ERN2 could be very accurately modelled and the structural effects of L-->F with or without R-->H computed by submission to online SwissProt modelling service.

Monodelphis ERN2 (key exon: sarHar2) aligned to human ERN1 luminal domain 
 Expect = 5.8e-65 Identities = 109/180 (60%), Positives = 141/180 (78%)

ERN2_monDom   1  PESLLFISTLDGSLHAVSKKTGDIQWTLKDDPIIQGPVYATEPAFLPDPSDGSLYILGEE  60
                 PE+LLF+STLDGSLHAVSK+TG I+WTLK+DP++Q P +  EPAFLPDP+DGSLY LG +
ERN1_homSap   8  PETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSK  67

ERN2_monDom  61  SKQGLMKLPFTIPELVHASPCHSSDGVFYTGRKQDTWFMVDPKSGKKQTMLSTETWDGLY  120
                 + +GL KLPFTIPELV ASPCRSSDG+LY G+KQD W+++D  +G+KQ  LS+   D L 
ERN1_homSap  68  NNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLC  127

ERN2_monDom  121 PSAPLLYIGRTQYTVTMYDPRSQALRWNTTYRGYSAPLLDHLPGYQVGHFTCSGEGLVVT  180
                 PS  LLY+GRT+YT+TMYD +++ LRWN TY  Y+A L +    Y++ HF  +G+GLVVT
ERN1_homSap  128 PSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVT  187

Functional significance: A considerable amount is known about the paralog ERN1. Annotation transfer is likely applicable to ERN2. The two gene products differ primarily in expression -- ERN1 ubiquitious but ERN2 restricted to intestinal epithelial cells:

"The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1 or ERN1) and PKR-related ER kinase (PERK) are two type I transmembrane ER-localized protein kinase receptors that signal the UPR through a process that involves homodimerization and autophosphorylation... The monomer of the luminal domain comprises a unique fold of a triangular assembly of beta-sheet clusters. Structural analysis identified an extensive dimerization interface stabilized by hydrogen bonds and hydrophobic interactions... Mutations that disrupt the dimerization interface produced ERN1 protein that failed to either dimerize or activate the UPR upon ER stress."

"ERN1 is a type I transmembrane protein kinase receptor that also has a site-specific RNase activity that, upon activation, initiates a site-specific unconventional splicing reaction. The substrate for IRE1 RNase in metazoans is Xbp1 mRNA, which encodes a basic leucine zipper transcription factor of the ATF/CREB family. XBP1 controls expression of genes containing an X-box element or a UPR element in their promoter regions. The IRE1-mediated splicing reaction introduces into XBP1 an alternative C terminus, thereby generating an XBP1 molecule that is a more potent transcriptional activator. Therefore, activation of IRE1 and its RNase increases the transcription of genes encoding ER chaperones and folding catalysts... the ERN1 N-terminal luminal domain (NLD) functions as an ER stress sensor... under normal conditions IRE1 is maintained in a monomeric state through interaction of the NLD with the ER resident chaperone BiP. Upon ER stress, Grp78 binds to unfolded proteins as they accumulate, permitting the released NLD to form homodimers. Dimerization of the NLD in turn leads to the activation of the protein kinase and RNase activities in the cytosolic domain of ERN1."

ENR2 is readily distinguished from its ERN1 paralog at tBlastn by including the two following exons which bring percent identity to 62%:

ERN2_monDom KLPFTIPELVHASPCRSSDGVLYTGRKQDTWFMVDPKSGKKQTMLSTETWDGLYPSAPLLYIGRTQYTVTMYDPRSQALRWNTTYRGYSA
            KLPFTIPELV ASPCRSSDG+LY G+KQD W++VD  +G+KQ  LS+   + L PS  LLY+GRT+YT+TM+D +S+ LRWN TY  Y+A
ERN1_monDom KLPFTIPELVQASPCRSSDGILYMGKKQDIWYVVDLMTGEKQQTLSSAFAESLCPSTSLLYLGRTEYTITMFDTKSRELRWNATYFDYAA

The first alignment shows ERN2 orthologs in vertebrates, the second as difference relative to opossum, the third ERN1 orthologs.
The ancestral nature of the CpG hotspot is shown in nucleotides in the final columns.

                            ^     *                                ^     *                                 ^     *  
ERN2_homSap  KLPFTIPELVHASPCRSSDGVFYT   ERN2_homSa  .....................F..   ERN1_homSap  KLPFTIPELVQASPCRSSDGILYM  CG Human
ERN2_panTro  KLPFTIPELVHASPCRSSDGVFYT   ERN2_panTr  .....................F..   ERN1_panTro  KLPFTIPELVQASPCRSSDGILYM  CG Chimp
ERN2_ponAbe  KLPFTIPELVHASPCRSSDGVFYT   ERN2_ponAb  .....................F..   ERN1_ponAbe  KLPFTIPELVQASPCRSSDGILYM  -- Gorilla
ERN2_rheMac  KLPFTIPELVHASPCRSSDGVFYT   ERN2_rheMa  .....................F..   ERN1_rheMac  KLPFTIPELVQASPCRSSDGILYM  CG Orangutan
ERN2_calJac  KLPFTIPELVHASPCRSSDGVFYT   ERN2_calJa  .....................F..   ERN1_calJac  KLPFTIPELVQASPCRSSDGILYM  CG Rhesus
ERN2_tarSyr  KLPFTIPELVHASPCRSSDGVFYT   ERN2_tarSy  .....................F..   ERN1_tarSyr  KLPFTIPELVQASPCRSSDGILYM  CG Marmoset
ERN2_micMur  KLPFTIPELVHASPCRSSDGVFYT   ERN2_micMu  .....................F..   ERN1_micMur  KLPFTIPELVQASPCRSTDGILYM  CG Tarsier
ERN2_tupBel  KLPFTIPELVHASPCRSSDGVFYT   ERN2_tupBe  .....................F..   ERN1_otoGar  KLPFTIPELVQASPCRSSDGILYM  CG Mouse_lemur
ERN2_musMus  KLPFTIPELVHASPCRSSDGVFYT   ERN2_musMu  .....................F..   ERN1_tupBel  KLPFTIPELVQASPCRSSDGILYM  -- Bushbaby
ERN2_ratNor  KLPFTIPELVHASPCRSSDGVFYT   ERN2_ratNo  .....................F..   ERN1_musMus  KLPFTIPELVQASPCRSSDGILYM  CG TreeShrew
ERN2_cavPor  KLPFTIPELVHTSPCRSSDGVFYT   ERN2_cavPo  ...........T.........F..   ERN1_ratNor  KLPFTIPELVQASPCRSSDGILYM  CG Mouse
ERN2_speTri  KLPFTIPELVHASPCRSSDGVFYT   ERN2_speTr  .....................F..   ERN1_dipOrd  KLPFTIPELVQASPCRSSDGILYM  CG Rat
ERN2_oryCun  KLPFTIPELVHASPCRSSDGVFYT   ERN2_oryCu  .....................F..   ERN1_cavPor  KLPFTIPELVQASPCRSSDGILYM  -- Kangaroo_rat
ERN2_ochPri  KLPFSIPELVHASPCRSSDGVFYT   ERN2_ochPr  ....S................F..   ERN1_speTri  KLPFTIPELVQASPCRSSDGILYM  CG Guinea_pig
ERN2_turTru  RLPFTIPELVHASPCRSSDGVFYT   ERN2_turTr  R....................F..   ERN1_oryCun  KLPFTIPELVQASPCRSSDGILYM  CG Squirrel
ERN2_bosTau  RLPFTIPELVHASPCRSSDGVFYT   ERN2_bosTa  R....................F..   ERN1_vicPac  KLPFTIPELVQASPCRSSDGILYM  CG Rabbit
ERN2_equCab  KLPFTIPELVHASPCRSSDGVFYT   ERN2_equCa  .....................F..   ERN1_turTru  KLPFTIPELVQASPCRSSDGILYM  CG Pika
ERN2_felCat  RLPFTIPELVHASPCRSSDGVFYT   ERN2_felCa  R....................F..   ERN1_bosTau  KLPFTIPELVQASPCRSSDGILYM  -- Alpaca
ERN2_canFam  KLPFTIPELVHASPCRSSDGVFYT   ERN2_canFa  .....................F..   ERN1_equCab  KLPFTIPELVQASPCRSSDGILYM  CG Dolphin
ERN2_myoLuc  KLPFTIPELVHASPCRSSDGVFYT   ERN2_myoLu  .....................F..   ERN1_canFam  KLPFTIPELVQASPCRSSDGILYM  CG Cow
ERN2_eriEur  KLPFTVPELVHTSPCRSSDGVFYT   ERN2_eriEu  .....V.....T.........F..   ERN1_myoLuc  KLPFTIPELVQASPCRSSDGILYM  CG Horse
ERN2_sorAra  KLPFTIPELVHASPCRSSDGVFYT   ERN2_sorAr  .....................F..   ERN1_pteVam  KLPFTIPELVQASPCRSSDGILYM  CG Cat
ERN2_loxAfr  KLPFTIPELVHASPCRSSDGVFYT   ERN2_loxAf  .....................F..   ERN1_eriEur  KLPFTIPELVQASPCRSSDGILYM  CG Dog
ERN2_echTel  KLPFTIPELVLASPCRSSDGVFYT   ERN2_echTe  ..........L..........F..   ERN1_sorAra  KLPFTIPELVQASPCRSSDGILYM  CG Microbat
ERN2_dasNov  KLPFTIPELVHTSPCRSSDGIFYT   ERN2_dasNo  ...........T........IF..   ERN1_loxAfr  KLPFTIPELVQASPCRSSDGILYM  -- Megabat
ERN2_monDom  KLPFTIPELVHASPCRSSDGVLYT   ERN2_monDo  KLPFTIPELVHASPCRSSDGVLYT   ERN1_proCap  KLPFTIPELVQASPCRSSDGILYM  CG Hedgehog
ERN2_macEug  KLPFTIPELVHASPCRSSDGVFYT   ERN2_macEu  .....................F..   ERN1_echTel  KLPFTIPELVQASPCRSSDGILYM  CG Shrew
ERN2_sarHar1 KLPFTIPELVQASPCRSSDGIFYM   ERN2_sarHa  ..........Q.........IF.M   ERN1_dasNov  KLPFTIPELVQASPCRSSDGILYM  -- Elephant
ERN2_sarHar2 KLPFTIPELVQASPCHSSDGIFYM   ERN2_sarHa  ..........Q....H....IF.M   ERN1_choHof  KLPFTIPELVQASPCRSSDGILYM  -- Rock_hyrax
ERN2_ornAna  KLPFTIPELVQSSPCRSSDGILYT   ERN2_ornAn  ..........QS........I...   ERN1_monDom  KLPFTIPELVQASPCRSSDGILYM  CG Tenrec
ERN2_anoCar  KLPFTIPELVQSSPCRSSDGIIYT   ERN2_anoCa  ..........QS........II..   ERN1_ornAna  KLPFTIPELVHASPCRSSDGILYM  CG Armadillo
ERN2_taeGut  KLPFTIPELVQSSPCRSSDGVLYT   ERN2_taeGu  ..........QS............   ERN1_galGal  KLPFTIPELVQASPCRSSDGILYM  CG Opossum
ERN2_galGal  KLPFTIPELVQASPCRSSDGILYM   ERN2_galGa  ..........Q.........I..M   ERN1_taeGut  KLPFTIPELVQASPCRSSDGILYM  CG Platypus
ERN2_xenTro  KLPFTIPELVQSSPCRSSDGILYT   ERN2_xenTr  ..........QS........I...   ERN1_anoCar  KLPFTIPELVQASPCRSSDGILYM  CG Lizard
ERN2_xenLae  KLPFTIPELVQSSPCRSSDGILYT   ERN2_xenLa  ..........QS........I...   ERN1_xenTro  KLPFTIPELVQSSPCRSSDGILYT  CG Tetraodon
ERN2_tetNig  KLPFTIPELVQASPCRSSDGVLYM   ERN2_tetNi  ..........Q............M   ERN1_tetNig  KLPFTIPELVQASPCRSSDGVLYM  CG Fugu
ERN2_takRub  KLPFTIPELVQASPCRSSDGVLYM   ERN2_takRu  ..........Q............M   ERN1_takRub  KLPFTIPELVQASPCRSSDGVLYM  CT Stickleback
ERN2_gasAcu  KLPFTIPDLVQSAPCRSSDGILYT   ERN2_gasAc  .......D..QSA.......I...   ERN1_gasAcu  KLPFTIPELVQASPCRSSDGVLYM  CT Medaka
ERN2_oryLat  KLPFTIPELVQSAPCRSSDGILYT   ERN2_oryLa  ..........QSA.......I...   ERN1_oryLat  KLPFTIPELVQASPCRSSDGVLYM  CG Lamprey
ERN2_calMil  KLPFTIPELVQSSPCRSSDGILYT   ERN2_calMi  ..........QS........I...   ERN1_danRer  KLPFTIPELVQASPCRSSDGILYM  
ERN2_petMar  KLPFTIPELVHASPCRTSDGVLYT   ERN2_petMa  ................T.......    
ERN_braFlo   KLPFTIPELVNASPCKSSDGILYT   ERN_braFlo  ..........N....K....I...

Case of MGAT5

chr4_4859 MGAT5 12 
>contig00001  length=538   numreads=5 21 C=2(61) Y=2(56)
LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE
.................................................
                     ^
Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5
and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in two
tasmanian  devil (here one is identical and the other differs from Monodelphis by C->Y) and finally the number of experimental reads that confirm the nucleotide
difference and the sum of the quality scores. The sequences were assembled by Newbler (the official 454 assembler).

Pseudogene issues: No processed pseudogenes relevent to this exon are seen by Blat of human and opossum sequence. Some questionable sequence occurs in tarsier and sloth but may be due to low coverage read or assembly error. These fragmentary sequences also have cysteine at the position in question.

Paralog issues: This gene has a moderately similar paralog, MGAT5B, with a similar enzymatic role (beta1,6-N-acetylglucosaminyltransferase). The opossum MGAT5B protein differs at 12 positions out of 49 from opossum MGAT5, whereas human and marsupial MGAT5A differ at one residue. Consequently the two paralogs are readily distinguished within vertebrates. This is moot because 33 of 33 available MGAT5B also have cysteine at the position in question (data not shown).

Homoplasy (recurrent mutation) issues: The alignments below show tyrosine has never replaced cysteine in any other species. This cysteine is extremely invariant in both paralogs, tracing back to lophotrochozoa and cnidaria.

Known variations: No human disease alleles have been mapped to either paralog. None of 9 SNP tracks at the UCSC browser show human variation in this exon.

Side issues: The column marked with an asterisk in the difference alignment below indicates a non-conservative phyloSNP K-->I that occured in the theran mammal stem after platypus divergence. All three marsupial sequences including tasmanian devil have isoleucine in this position as do all 30 of the available placental mammal sequences, suggesting that both the lysine and the isoleucine continue to be under strong selection. No comparable shift occured in the theran stem for MGAT5B where the residue is arginine in all species, a basic residue similar to lysine.

Structural significance: The MGAT5 gene supposedly encodes a conventional enzyme, mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Yet surprisingly, no determined 3D structure exists at PDB relevent to the configuration of this exon -- nor indeed the large 741 residue protein. This is very peculiar because glycosyl transerfases are a well-studied group of enzymes (nearly 100 loci in human) and might be expected to bind UDP-GlcNAc (like MGAT4A or MGAT3).

Only a small region of the protein have a prediction at ModBase using 2f9fA, a remote mannosyltransferasee from Archaeoglobus fulgidus. Luckily the model covers the cysteine at issue, showing two helices and a beta sheet.

SwissProt does not annotate the cysteine at position 532 as part of a disulfide or active site; the predicted location (Golgi) can have homodimer disulfides of similar enzymes, though this is a complex topic. Although all 20 cysteines in this protein are conserved human to opossum, this could be a consequence of the overall sequence identity of 90%. Twelve of the cysteines, not including the Sarcophilus variant, are found in the last 140 residues, perhaps forming a disulfide knot. All but 1 of these cysteines is conserved in the pre-Bilateran anemone Nematostella (which enriches relative to overall percent identity of 43%).

Highest MGAT5 expression occurs in brain, heart, kidney, and placenta. No domains other than a signal peptide and 6 of its own glycosylation target sites are found by online tools such as SMART.

Although the bulky tyrosine substitution is conservative in the sense of polar nature and perhaps hydrogen-bonding capacity, it cannot replace these specialized functions of cysteine. Considering the extreme conservation of this cysteine, this substitution must have a substantial-- perhaps even disabling -- impact on enzymatic function.

Functional significance: In view of the facial tumor situation in tasmanian devils, OMIM's account of prior research in mouse on this gene is quite interesting. Less is known about MGAT5B though it also functions in the synthesis of complex cell surface N-glycans.

" Malignant transformation is accompanied by increased beta-1,6-GlcNAc branching of N-glycans attached to Asn-X-Ser/Thr sequences in mature glycoproteins... The amount of MGAT5 products correlates with disease progression... Mgat5-deficient mice, which are born healthy but develop various abnormalities as adults...Mgat5-deficient mice showed kidney autoimmune disease, enhanced delayed-type hypersensitivity, and increased susceptibility to experimental autoimmune encephalomyelitis...The Golgi enzyme beta1,6 N-acetylglucosaminyltransferase V (Mgat5) is up-regulated in carcinomas and promotes the substitution of N-glycan with poly N-acetyllactosamine, the preferred ligand for galectin-3 (Gal-3)...inhibitors of MGAT5 might be useful in the treatment of malignancies by targeting their dependency on focal adhesion signaling for growth and metastasis."

                                   ^                                                  ^                   *
MGAT5_homSap  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  homSap
MGAT5_panTro  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  panTro
MGAT5_gorGor  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  gorGor
MGAT5_ponAbe  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  ponAbe
MGAT5_rheMac  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  rheMac
MGAT5_calJac  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  calJac
MGAT5_micMur  LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE  .............................S...................  micMur
MGAT5_otoGar  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  otoGar
MGAT5_tupBel  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  tupBel
MGAT5_musMus  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  musMus
MGAT5_ratNor  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  ratNor
MGAT5_criGri  LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE  .............................S...................  criGri
MGAT5_dipOrd  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  dipOrd
MGAT5_cavPor  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  cavPor
MGAT5_speTri  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  speTri
MGAT5_oryCun  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  oryCun
MGAT5_ochPri  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  ochPri
MGAT5_vicPac  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  vicPac
MGAT5_susScr  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  susScr
MGAT5_turTru  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  turTru
MGAT5_bosTau  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  bosTau
MGAT5_equCab  LFAGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  ..A..............................................  equCab
MGAT5_felCat  lfvgLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE  .............................S...................  felCat
MGAT5_canFam  LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE  .............................S...................  canFam
MGAT5_myoLuc  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  myoLuc
MGAT5_eriEur  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  eriEur
MGAT5_sorAra  LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE  .............................S...................  sorAra
MGAT5_loxAfr  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  loxAfr
MGAT5_proCap  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  proCap
MGAT5_echTel  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE  .................................................  echTel
MGAT5_monDom  LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE  ..........................V......................  monDom
MGAT5_macEug  LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE  ..........................V......................  macEug
MGAT5_sarHar1 LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE  ..........................V......................  sarHar1
MGAT5_sarHar2 LFVGLGFPYEGPAPLEAIANGYAFLNVKFNPPKSSKNTDFFIGKPTLRE  .....................Y....V......................  sarHar2
MGAT5_ornAna  LFVGLGFPYEGPAPLEAIANGCAFLNLKFNPPKSSKNTDFFKGKPTLRE  ..........................L..............K.......  ornAna
MGAT5_galGal  LFVGLGFPYEGPAPLEAIANGCAFLNLRFNPPKSSKNTEFFKGKPTLRE  ..........................LR..........E..K.......  galGal
MGAT5_taeGut  LFVGLGFPYEGPAPLEAIANGCAFLNLRFNPPKSSKNTDFFKGKPTLRE  ..........................LR.............K.......  taeGut
MGAT5_anoCar  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFKGKPTLRE  .........................................K.......  anoCar
MGAT5_xenTro  LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSRNTDFFKGKPTLRE  ...................................R.....K.......  xenTro
MGAT5_tetNig  VFVGLSFPYEGPAPLEALANGCIFLNPRLKPPQSSLNSEFFKEKPNIRE  V....S...........L....I....RLK..Q..L.SE..KE..NI..  tetNig
MGAT5_takRub  LFVGLSFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFKGKPTLRE  .....S...................................K.......  takRub
MGAT5_gasAcu  LFVGLSFPYEGPAPLEAIANGCAFLNPKFSPAKSSKNTDFFKGKPTLRE  .....S.......................S.A.........K.......  gasAcu
MGAT5_oryLat  LFVGLSFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFKGKPTLRE  .....S...................................K.......  oryLat
MGAT5_danRer  LFVGLSFPYEGPAPLEAIANGCAFLNPRFDPAKSSKNTDFFKGKPTLRE  .....S.....................R.D.A.........K.......  danRer
MGAT5_oncMyk  LFVGLSFPYEGPAPLEAIANGCAFLNPKFTPPKSSKNTDFFKGKPTLRE  .....S.......................T...........K.......  oncMyk
MGAT5_pimPro  LFVGLSFPYEGPAPLEAIANGCAFLNPRFDPSKSSKNTDFFKGKPTLRE  .....S.....................R.D.S.........K.......  pimPro
MGAT5_calMil  LFVGLGFPYEGPAPLEAIANGCAFLNPRFNPPKSSKNTEFFKGKPTLRE  ...........................R..........E..K.......  calMil
MGAT5_petMar  LFVGLGFPYEGPAPLEAIANGCVFLNPRFRPPKSSKNTDFFKGKPTLRE  ......................V....R.R...........K.......  petMar
MGAT5_braFlo  LFVGLGFPYEGPAPLEAIASGCVFLNPKFTQPKSRLNTKFFEGKPTFRE  ...................S..V......TQ...RL..K..E....F..  braFlo
MGAT5_strPur  LFIGLGFPYEGPAPLEAVANGCVFLNPKFNPPKNYQNTKFFQGKPTSR.
MGAT5_helRob  LFIGLGFPYEGPAPLEAIAAGCVFINPKFNPPHSSLNTKFFKGKPTARE
MGAT5_nemVec  VFIGLGFPYEGPAPLEAIQSGCVFLNAKFDPPHDRVNTPFFKNKPTLRK

Note: the species with unfamiliar genSpp acronyms are Cricetulus griseus, Oncorhynchus mykiss, Pimephales promelas , Callorhinchus milii, Branchiostoma floridae, Strongylocentrotus purpuratus, Helobdella robusta, Nematostella vectensis, and Acropora millepora.

Here the opossum protein is broken into its 16 coding exons with phases (base overhangs at split codons) shown:

>MGAT5_monDom length=743
0 MAFFAPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQHESSSMLREQILDLSKRYIKALAEENRNVVDGPYVGVMTAY 1
2 DLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTTTTAAPSSIAAFEKISVA 1
2 DIINGAQEKCELPPMDGFPHCEGKIK 0
0 WMKDMWRTDPCYANYGVDGSTCSFFIYLSE 0
0 VENWCPHLPWRAKNPYEEPDQNSM 0
0 AEIRTDFNLLYGMMKRHEEFRWMILRIRRMADAWIEAIKSLAEKQNLEKRKRKK 0
0 ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE 2
1 IMKRVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQ 2
1 CMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMF 1
2 PHTPDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDSFWK 0
0 NKKEYLDIIHTYMEVHATVYGSSTNHMPSYVKNHGILSGRDLQFLLRETK 0
0 LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE 0
0 LTSQHPYAEVFIGRPHVWTVNPTDHREVENAVKAILNQK 0
0 IEPYMPYEFTCEGMLQRMNAFIEKQ 0
0 DFCHGQVMWPPLNALQVKLSEPGKSCKQVCQENQLICEPSFFQHLNKDKDVLK 2
1 YEVICHTTELANDILVPSYDDKKKHCVFQGDLLLFSCAGAHPKHKRICPCRDYIKGQVALCQDCL* 0

>MGAT5_sacHar Sarcophilus harrisii (tasmanian_devil) one match to exon 1: FPUIIJ301C96S1
0 MAFFAPWKLSSQN*GFSWLTFGFIWGMMLLHFTIQQRTQHESSSMLREQILDLSKRYIKALAEENRNVVDGPYVGVMTAY 1
2 DLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTTTTAVPSSIAAFEKIsVA 1
2 DIINGAQEKCELPPMDGFPHCEGKIK 0
0  0
0  0
0 AEIRTDFHLLYGMMKRHEEFRWMILRIRRMADAWIEAIKSLAEKQNLEKRKRKK 0
0 ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE 2
1 IMKRVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQ 2
1 CMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMF 1
2 AHTPDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDNFWK 0
0 NKKEYLDIIHTYMEVHATVYGSSTNHMPSYVKNHGILSGRDLQFLLRETK 0
0 LFVGLGFPYEGPAPLEAIANGYAFLNVKFNPPKSSKNTDFFIGKPTLRE 0
0  0
0 IEPYMPYEFTCEGMLQRMNAFIEKQ 0
0  2
1 YEVVCHTTELANDILVPSYDDRKKHCVFQGDLLLFSCAGAHPKHKRICPCRDYIKGQVALCQDCL* 0

The premature stop codon in the first exon is likely read error (1 bp dropped, 1 bp later added):

atggctttctttgctccatggaaattatcctctcagaaactagggtttttcctggtgact
 M  A  F  F  A  P  W  K  L  S  S  Q  K  L  G  F  F  L  V  T    correct monDom frame
  W  L  S  L  L  H  G  N  Y  P  L  R  N  -  G  F  S  W  -  L   6 residue observed frameshifts in sarHar N*GFSWL
   G  F  L  C  S  M  E  I  I  L  S  E  T  R  V  F  P  G  D  F  irrelevent 3rd reading frame

MGAT5 has 16 exons. The key one here is 12. Alignment of MGAT5_sarHar to opossum shows only 5 differences in 589 residues available for comparison. 
Alignment of Monodelphis to human establishes that MGAT5 is better conserved than the average gene: 

Identities = 673/744 (90%), Positives = 708/744 (95%), Gaps = 2/744 (0%)

monDo  1     MAFFAPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQHESSSMLREQILDLSKRYIKA  60
             MA F PWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQ ESSSMLREQILDLSKRYIKA
homSa  146   MALFTPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSSMLREQILDLSKRYIKA  325

monDo  61    LAEENRNVVDGPYVGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTT  120
             LAEENRNVVDGPY GVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLV+   G  + +T 
homSa  326   LAEENRNVVDGPYAGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVV--NGTGTNSTN  499

monDo  121   TTAAPSSIAAFEKISVADIINGAQEKCELPPMDGFPHCEGKIKWMKDMWRTDPCYANYGV  180
             +T A  S+ A EKI+VADIINGAQEKC LPPMDG+PHCEGKIKWMKDMWR+DPCYA+YGV
homSa  500   STTAVPSLVALEKINVADIINGAQEKCVLPPMDGYPHCEGKIKWMKDMWRSDPCYADYGV  679

monDo  181   DGSTCSFFIYLSEVENWCPHLPWRAKNPYEEPDQNSMAEIRTDFNLLYGMMKRHEEFRWM  240
             DGSTCSFFIYLSEVENWCPHLPWRAKNPYEE D NS+AEIRTDFN+LY MMK+HEEFRWM
homSa  680   DGSTCSFFIYLSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWM  859

monDo  241   ILRIRRMADAWIEAIKSLAEKQNLEKRKRKKILVHLGLLTKESGFKIAENAFSGGPLGEL  300
              LRIRRMADAWI+AIKSLAEKQNLEKRKRKK+LVHLGLLTKESGFKIAE AFSGGPLGEL
homSa  860   RLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGEL  1039

monDo  301   VQWSDLITSLYLLGHDIRISASLAELKEIMKRVVGNRSGCPTVGDRIVELIYIDIVGLAQ  360
             VQWSDLITSLYLLGHDIRISASLAELKEIMK+VVGNRSGCPTVGDRIVELIYIDIVGLAQ
homSa  1040  VQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQ  1219

monDo  361   FKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHT  420
             FKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHT
homSa  1220  FKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHT  1399

monDo  421   PDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDSFWKNKKEYLDIIHTYMEVHAT  480
             PDNSFLGFVVEQHLNSSDI HINEIKRQNQSLVYGKVDSFWKNKK YLDIIHTYMEVHAT
homSa  1400  PDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHAT  1579

monDo  481   VYGSSTNHMPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNVK  540
             VYGSST ++PSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLN K
homSa  1580  VYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPK  1759

monDo  541   FNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVNPTDHREVENAVKAILNQK  600
             FNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTV+  +  EVE+AVKAILNQK
homSa  1760  FNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQK  1939

monDo  601   IEPYMPYEFTCEGMLQRMNAFIEKQDFCHGQVMWPPLNALQVKLSEPGKSCKQVCQENQL  660
             IEPYMPYEFTCEGMLQR+NAFIEKQDFCHGQVMWPPL+ALQVKL+EPG+SCKQVCQE+QL
homSa  1940  IEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQL  2119

monDo  661   ICEPSFFQHLNKDKDVLKYEVICHTTELANDILVPSYDDKKKHCVFQGDLLLFSCAGAHP  720
             ICEPSFFQHLNKDKD+LKY+V C ++ELA DILVPS+D K KHCVFQGDLLLFSCAGAHP
homSa  2120  ICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHP  2299

monDo  721   KHKRICPCRDYIKGQVALCQDCL*  744
             +H+R+CPCRD+IKGQVALC+DCL 
homSa  2300  RHQRVCPCRDFIKGQVALCKDCL*  2371

Full length genes appear available from GenBank and genome projects for mouse, rat (NM_001107068), dog (wgs exons), horse (XM_001489091), wallaby (wgs exons), and platypus (XM_001520380). Because this gene is 90% conserved at marsupial, placental mammals will not be informative -- indeed it is necessary to go to greater phylogenetic depth than lamprey to define the ultra-conserved residues in this protein:

>MGAT5_macEug nearly identical to monDom; 3 exons are missing, 2 partial exons, exon 4 has frameshifts 
0 MAFFAPWKLSSQKLGFFL   1
2 DLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTTTTAVPSSIAAFEKISVA 1
2 DIINGAQEKCELPPMDGFPHCEGKIK 0
0 WMKDiWRTDPCYANYGVDGSTCSFFIYLSE 0
0 VENWCPHLPWRAKNPYEEPDQNSM 0
0 0
0 ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE 2
1 2
1    GTEPEFNHANYAQSKGHKTP    1
2 aHTPDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDSFWK 0
0 NKKEYLDIIHTYMEVHATVYGSSTNHMPSYVKNHGILSGRDLQFLLRETK 0
0 LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE 0
0 LTSQHPYAEVFIGRPHVWTVNPTDHREVENAVKAILNQK 0
0 IEPYMPYEFTCEGMLQRMNAFIEKQ 0
0 DFCHGQVMWPPLNALQVKLSEPGKSCKQVCQENQLICEPSFFQHLNKDKDVLK 2
1 YEVICHTTELANDILVPSYDDKKKHCVFQGDLLLFSCAGAHPKHKRICPCRDYIKGQVALCQDCL* 0

>MGAT5_galGal 87% identical to opossum
MAFPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQQTQHESSSVLREQILDLSKRYIKALAEENKNVVDGPYVGTVTAY
DLKKTLAVLLDNILQRIGKLESKVENLVLNGTGANSTNTTTPAPSLGAVEKLNVA
DLINGAQEQCELPPMDGFPHCEGKIK
WMKDMWRSDPCYASYGVDGSTCSFFIYLSE
VENWCPRLPWRAKNPNEETDQKTV
AEIRINFDPLYKMMSRHEEFRWMTLRIRRMADTWIEAIKSLAEKQNLENRKRKK
ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE
IMKKVVGNRSGCPTQGDKVVELIYIDIVGLTQFKKTLGPSWVHYQ
CMLRVLDSFGTEPEFNHAHYAQSKGHKTPWGKWNLNPQQFYTMF
PHTPDNSFLGFVVEQHLNSSDIKHINDIKRQNQSLVYGKVDNFWK
DKKAYLDVIHTYMEVHGTVHGTSTIYIPGYVKNHGILSGRDLQFLLRETK
LFVGLGFPYEGPAPLEAIANGCAFLNLRFNPPKSSKNTEFFKGKPTLRE
LTSQHPYAEVYIGKPHVWTVDINNLSEVEKAVKSILNQK
IDPYLPYEFTCEGMLQRMNAFIERQ
DFCHGQVMWPPLSALQVKLAEPGKSCKQVCQESQLICEPSFFQHLNKDKALLK
HNIECLTTESANDILVPSFDGRRKHCVFQGDLLLFSCAGSHPTHRRICPCRDYIKGQVALCKDCL*

>MGAT5_nemVec Nematostella vectensis (sea anemone) XM_001641404 43% identical to opossum 19 of 20 cysteines conserved
MIATKGRPTFKLSAHRIGIVFIIISFIWGLYLIKIQLDERNSQPDYLKGRIIHLSKEYIRALAREKGVYGIDGQPSTQQGVGDLKKATAVLLQSMLERIHVL
EKQVEGVIVNSTLEFEILASQIKSLNTTFSLHLSNHSYVSANSCVIPDDPSYPECRQKVMWMRNFWKTHECYAKDHGVNGTICSFLVYLSEVENWCPKFPGRMKPTSRATTEGADL
HRSDVQGLLGLLNDQDPIKFKWIKNRINQMWPQWLSALEDLKKKRDLKKIKQKKILVHIGLLANERALHFAANADKGGPLGELVQWSDLIASLYLLGHDVTVTADIPRLQGIFGKL
RGPAKKPCPTTIKNDYDLIYLDYYGVKQMQTKVGQFTQSFKCKFRIVDSFGTEAQFNYAGFTEKVPGGSMALWGRHNLNLKQFMTMFPHSPDNSFLGFVVGEEPTPDPHPKKKKAR
ALVYGKHYYMWKDLKQRSFLDVINKYMEIHATVGGGIKKWVPSYVINHGVLPSLEVQKLLQDSMIFVGLGFPYEGPAPLEAIAHGCFFLNTKYHPPRNRINTPFFKDKPTLRQITS
QHPYAEDYIGQPYVYTVDINDLNKIEAVMKEIMMAEPVSPYLPYEFTHKGMLERLHVFIENQNFCGQNLWPPLNALQARKGAMGSSCKETCHSLGLVCEPQYFPAINTKERMTRSG
FPCNTTRVEDMPSLVAPGYRDDPPVCLRQAQNLLFSCTANSPTTKRLCPCRDFKKGQVALCSKC*

Case of ACTL6B

chr2_18546 ACTL6B 11  
>contig00001 length=502 numreads=11
GLSGNTMLGVGHVVTTSIGMCDIDIRP
...........................
   ^
3 G=4(94) R=7(213)

Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5
and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in
tasmanian  devil (in this case, one individual differs from Monodelphis by G->R), then differences between the two devils, and finally the number of experimental reads that confirm the nucleotide
difference and the sum of the quality scores. The sequences were assembled by Newbler.

The change from small non-polar glycine to bulky positively charged lysine is highly non-conservative, especially at a highly conserved residue such as this. Again the change in Sarcophilus is at a CpG hotspot, this time with a mildly unusual transversion of the C to the purine G.

The well-studied protein here is a member of a family of actin-related proteins (ARPs) which have significant homology to conventional actins, in particular sharing the actin fold (an ATP-binding cleft) as common feature. ACTL6B and its 83% identical paralog ACTL6A are involved in diverse cellular processes such as vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. Both have 14 coding exons. The entire exon containing the G-->R is highly conserved including the glycine.

Pseudogene issues: Blat of full length sequence to human shows no recent processed or segmental pseudogenes. However more sensitive methods show a half dozen processed pseudogenes on different chromosomes plus one for ACTL6A. And opossum assembly, which has all 14 exons, also contains a fairly recent processed pseudogene with 91.5% identity. This locus has internal stop codons and ELSD in place of GLSG for the key glycine. This pseudogene arose from ACTL6A, not ACTL6B.

Retroposed Genes, Including Pseudogenes (retroMrnaInfo UCSC track):

ACTL6B at chrX:53188763-53189824     ACTL6B at chr9:110656744-110657692    ACTL6A at chr14:49217726-49219292
ACTL6B at chr7:5533936-5535808       ACTL6B at chr6:46280879-46281761 
ACTL6B at chr17:77092347-77093972    ACTL6B at chr1:227633849-227635482

Sarcophilus also has one or more processed pseudogenes which considerably complicates the interpretation of tblastn output. However reads FP1I63R01ARR6N etc show two consecutive exons, the first of which is the G-->R version of the exon and the second identical to the following exon from opossum. The spacing between the two exons is 132 bp, more than adequate for a mammalian intron (whose lower limit is about 78 bp). Other reads span two exon for the normal version of the exon such as FKUJDAX01DZSZO etc again with same intron spacing. (Processed pseudogenes may later acquire pseudo-introns in the form of retroposons so RepeatMaskers needs to be run on the intervening sequence.)

>FP1JAYN01EIJD3 length=493 xy=1734_1049 region=1 run=R_2009_01_29_12_22_00_

monDo: 37  VKGLSGNTMLGVGHVVTTSIGMCDIDIRP 65
           ++GLS NTMLGVGHVVTTSIGMCDIDIRP
sacHa: 386 LQGLSRNTMLGVGHVVTTSIGMCDIDIRP 300

monDo: 66  GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP 97
           GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP
sacHa: 168 GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP 73

Newbler has a bad tendency to create faux frameshifts:

Query: 82  ggtctctacggcagtgtcattgtcactggagggaacacactcttgcaaggctttactgac 141 
           |||||||||||||||||||||| |||||||||||||||||| |||||||| |||||||||
Sbjct: 167 ggtctctacggcagtgtcattg-cactggagggaacacactgttgcaaggttttactgac 109 FP1I63R01APY7E 

Query: 82  ggtctctacggcagtgt-cattgtcactggagggaacacactcttgcaaggctttactga 140
           ||||||||||||||||| |||||||||||||||||||||||| |||||||| ||||||||
Sbjct: 268 ggtctctacggcagtgttcattgtcactggagggaacacactgttgcaaggttttactga 327 FKUJDAX01AWWZ3

Query: 82  ggtctctacggcagtgtcattgtcactggagggaac-acactcttgcaagg 131
           |||||||||||||||||||||||||||||||||||| ||||| ||||||||
Sbjct: 268 ggtctctacggcagtgtcattgtcactggagggaacgacactgttgcaagg 318 FKUJDAX01DZSZO
 

Paralog issues: There is potential for confusion with the paralog ACTL6A. This wouldn't normally matter because all species in this gene too have glycine at the arginine-substituted site. However its pseudogene could present problems because its decay may have taken a different path in Sarcophilus than in Monodelphis giving the R (instead of D), assuming the pseudogene was formed prior to divergence of these species. Indeed, Macropus eugenii appears to have two processed pseudogenes; one of this has R in place of a glycine 4 residues earlier. It will prove necessary to consider adjacent regions in Sarcophilus reads to determine whether the feature is a pseudogene.

To summarize, this appears to be a valid coding SNP but the situation with paralogs, pseudogenes, and errors intrinsic to the 454 platform makes it unfavorable for rapid screening. It would be necessary to require matches of flanking intronic regions on both sides to be sure that the right locus is being investigated.

Comparison of gene to pseudogene in opossum:

000000889  E  R  L  R  I  P  E  G  L  F  D  P  S  N  V  K  G  L  S  G  000000948
<<<<<<<<<  |  X  |  K  |  |  |  |  |  |  |  |  |  |  |  |  E  |  |  D  <<<<<<<<<
250390825 gagtgactcaagattcctgaagggttatttgacccatctaatgtgaaggaattgtcagac 250390766

000000949  N  T  M  L  G  V  G  H  V  V  T  T  S  I  G  M  C  D  I  D  000001008
<<<<<<<<<  |  |  |  |  |  |  S  |  |  |  |  |  |  F  |  |  |  |  |  |  <<<<<<<<<
250390765 aacacaatgttgggagtcagtcatgttgttaccacaagctttgggatgtgtgacattgac 250390706

000001009  I  R  P  G  L  Y  G  S  V  I  V  T  G  G  N  T  L  000001059
<<<<<<<<<  F  |  |  |  |  |  D  N  M  L  G  A  |  |  |  I  |  <<<<<<<<<
250390705 tttagaccgggactttatgacaatatgttaggggcgggaggaaacattctg 250390655

Comparison of ACTL6A_homSap gene to pseudogenes in wallaby:

macEu:  1063 FPVGYNCNFGVEQLKITERLFDPSNVKRLSGNPMLGVSHVVTTRIGMCDIDIRPGLYGTV 1242
             FP GYNC+FG E+LKI E LFDPSNVK LSGN MLGVSHVVTT +GMCDIDIRPGLYG+V
homSa:   289 FPNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDIRPGLYGSV 348
 
macEu:    48 PNVYKCGFGAEHFKIPEGLFDRSNMKGLSGNTMLGISHVVTKSTGMCDIDIRPGFYISVI 227
             PN Y C FGAE  KIPEGLFD SN+KGLSGNTMLG+SHVVT S GMCDIDIRPG Y SVI
homSa:   290 PNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDIRPGLYGSVI 349

Homoplasy (recurrent mutation) issues:

Known variations:

Side issues:

Structural significance:

Functional significance:

                  *                            *                                            *
ACTL6B_homSap  GLSGNTMLGVGHVVTTSIGMCDIDIRP  GLSGNTMLGVGHVVTTSIGMCDIDIRP    ACTL6B_homSap GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_panTro  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_panTro GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_gorGor  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_gorGor GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_ponAbe  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_ponAbe GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_rheMac  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_rheMac GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_calJac  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_calJac GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_tarSyr  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_tarSyr GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_micMur  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_micMur GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_otoGar  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_otoGar GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_tupBel  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_tupBel GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_musMus  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_musMus GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_ratNor  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_ratNor GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_dipOrd  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_dipOrd GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_cavPor  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_cavPor GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_ochPri  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_ochPri GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_turTru  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_turTru GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_bosTau  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_bosTau GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_equCab  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_equCab GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_felCat  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_felCat GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_canFam  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_canFam GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_myoLuc  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_myoLuc GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_pteVam  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_pteVam GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_eriEur  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_eriEur GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_loxAfr  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_loxAfr GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_proCap  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_proCap GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_echTel  GLSGNTMLGVGHVVTTSIGMCDNDIRP  ......................N....    ACTL6B_echTel GLSGNTMLGVGHVVTTSIGMCDNDIRP
ACTL6B_monDom  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_monDom GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_ornAna  GLSGNTMLGVSHVVTTSVGMCDIDIRP  ..........S......V.........    ACTL6B_ornAna GLSGNTMLGVSHVVTTSVGMCDIDIRP
ACTL6B_galGal  GLSGNTMLGVSHVVTTSVGMCDIDIRP  ..........S......V.........    ACTL6B_galGal GLSGNTMLGVSHVVTTSVGMCDIDIRP
ACTL6B_taeGut  GLSGNTMLGVSHVVTTSVGMCDIDIRP  ..........S......V.........    ACTL6B_taeGut GLSGNTMLGVSHVVTTSVGMCDIDIRP
ACTL6B_anoCar  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_anoCar GLSGNTMLGVGHVVTTSIGMCDIDIRP
ACTL6B_xenTro  GLSGNTMLGVSHVVTTSVGMCDIDIRP  ..........S......V.........    ACTL6B_xenTro GLSGNTMLGVSHVVTTSVGMCDIDIRP
ACTL6B_tetNig  GLSGNTMLGVSHVVTTSVGMCDIDIRP  ..........S......V.........    ACTL6B_tetNig GLSGNTMLGVSHVVTTSVGMCDIDIRP
ACTL6B_takRub  GLSGNTMLGVSHVVTTSVGMCDIDIRP  ..........S......V.........    ACTL6B_takRub GLSGNTMLGVSHVVTTSVGMCDIDIRP
ACTL6B_gasAcu  GLSGNTMLGVGHVVTTSVGMCDIDIRP  .................V.........    ACTL6B_gasAcu GLSGNTMLGVGHVVTTSVGMCDIDIRP
ACTL6B_oryLat  GLSGNTMLGVGHVVTTSVGMCDIDIRP  .................V.........    ACTL6B_oryLat GLSGNTMLGVGHVVTTSVGMCDIDIRP
ACTL6B_danRer  GLSGNTMLGVGHVVTTSIGMCDIDIRP  ...........................    ACTL6B_danRer GLSGNTMLGVGHVVTTSIGMCDIDIRP
                  *                            *                                            *
Consensus      gLsGnTMlgvgHVVTts!g$CDi.Ir.  gLsGnTMlgvgHVVTts!g$CDi.Ir.  

>ACTL6B_homSap
MSGGVYGG
DEVGALVFDIGSFSVRAGYAGEDCPK
ADFPTTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHVPRDGAEVMSPLKNGM
IEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAP
WNTRAKREKLTELMFEQYNIPAFFLCKTAVLTA
FANGRSTGLVLDSGATHTTAIPVHDGYVLQQ
GIVKSPLAGDFISMQCRELFQEMAIDIIPPYMIAAK
EPVREGAPPNWKKKEKLPQVSKSWHNYMCN
EVIQDFQASVLQVSDSPYDEQ
VAAQMPTVHYEMPNGYNTDYGAERLRIPEGLFDPSNVK
GLSGNTMLGVGHVVTTSIGMCDIDIRP
GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP
SMRLKLIASNSTMERKFSPWIGGSILASL
GTFQQMWISKQEYEEGGKQCVERKCP*

>ACTL6B_monDom
MSGGVYGG
DEVGALVFDIGSFSVRAGYAGEDCPK
ADFPTTVGLLTLEEGGGLELDGEKEKKGKTFHIDTNALHVPRDGAEVMSPLKNGM
IEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAP
WNTRAKREKLTELMFEQYNIPAFFLCKTAVLTA
FANGRSTGLVLDSGATHTTAIPVHDGYVLQQ
GIVKSPLAGDFISMQCRELFQEMAIDIIPPYMIAAK
EPVREGAPPNWKKKEKLPQVSKSWHNYMCN
EVIQDFQASVLQVSDSPYDEQ
VAAQMPTVHYEMPNGYNTDYGAERLRIPEGLFDPSNVK
GLSGNTMLGVGHVVTTSIGMCDIDIRP
GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP
SMRLKLIASNSTMERKFSPWIGGSILASL
GTFQQMWISKQEYEEGGKQCVERKCP*

Case of IPO7

chr5_9037 IPO7 23 
>contig00001  length=680   numreads=8
SSQVEKHSCSLTEELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ
....*N.....................................................F.....................
                                                           ^
59 F=2(72) S=3(53)

Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5
and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in
tasmanian  devil (in this case, both individuals differ from Monodelphis by  -> ), then differences between the two devils
(here one individual has S at position 59, the other has F), and finally the number of experimental reads that confirm the nucleotide
difference and the sum of the quality scores. 

Here the Ensembl-predicted sequence for opossum IPO7 is wrong. The exon begins with EELGSD... and the preceding residues are rubbish. The stop codon and N are thus extraneous.

Pseudogene issues: Human has 4 processed pseudogenes originating at various dates. However opossum lacks any detectable by Blat.

Retroposed Genes, Including Pseudogenes (from pseudoGeneLink and retroMrnaInfo UCSC tracks)
 IPO7 at chr1:209097616-209101414
 IPO7 at chr13:23593176-23594670
 IPO7 at chr20:25520871-25521227
 IPO7 at chrX:51680122-51682234

Paralog issues: IPO8 is somewhat similar but not sufficiently in this exon to engender confusion.

monDom7 EEIPSDEEDTNEARQALHE---RGGGEDEEEDDDDWDEEVLEETALEGFSTPLDLDDG-VDEYQFFT---QALLSRS
        EE+ SDE+D +E  Q   E   +  GED   DD++W+E+  EETALEG+ST +D ++  VDEYQ F    QA+ SR+  
monDom8 EELGSDEDDIDEDGQEYLEILAKQAGEDG--DDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQAIQSRN

sacHar7 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIF
        EE+ SDE+D +E  Q   E       ++ +D++W+ED  EETALEG+ST +D E++ VDEYQ F
sacHar8 EEIPSDEEDTNETSQTMHENNGGGDEDEEEDDDWDEDVLEETALEGFSTPLDLEDS-VDEYQFF 

Homoplasy (recurrent mutation) issues: It can be seen from the 44-species alignment that the serine here is quite invariant, being conserved in all amniotes. In frog and all earlier diverging species, threonine is utilized. However serine is used in all vertebrates at the comparable position in the paralog IPO8 except for tetraodon which again uses threonine, as do weaker homologs in the protostomes Tribolium and Ixodes and cnidarians Nematostella and Acropora. This could be described as a reduced alphabet situation where the residue is strongly restricted to a small residue with hydroxyl side chain. Phenylalanine here, as in Sarcophilus, is thus an immensely non-conservative change as it is bulkly, unable to hydrogen bond, and unsuitable for the protein surface.

Query  2     ELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYFTIIDDEENPVDEYQIFKAIFQ IPO7_sarHar
             EL SDED+I+ED  +Y+E LA +A E  DD++  E   EETALE + T +D EE  +DE+  F+   Q
Sbjct  1788  ELASDEDEINEDDVQYIESLALKAAEHLDDDDVCE---EETALENFTTSVDTEE--IDEFIAFRTSLQ Acropora millepora
 
Query  3      LGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYFTIIDDEENPVDEYQIFK IPO7_sarHar 
              L SDED+ +ED  EY+E LAK+A  D  D+E ++DD EET LE Y T ID E   +DEY  FK 
Sbjct  2611   LASDEDEFNEDDVEYIENLAKKAA-DHFDDEDDDDDDEETPLEEYTTSIDGEN--MDEYIAFK Nematostella vectensis 

Known variations: No disease variants are known according to OMIM for either IPO7 or IPO8. No relevent structure at PDB has been determined for the central or distal region of the protein. The protein is quite large and thus it will be very difficult to predict the environment of the serine, much less the impact of phenylalanine substitution.

Side issues: Importin IPO7 has a broad and extremely important function in nuclear protein import, either autonomously as nuclear transport receptor or as an adapter in association with KPNB1. Havilng a receptor for nuclear localization signals, it can promote translocation of import substrates through the nuclear pore complex (NPC) by the energy requiring RAN-dependent mechanism. It mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5, but in association with KPNB1 the import of five histones. The role of the paralog IPO8 is similar.

The question here is to what extent could IPO8 compensate for the S-->F change observed in Sarcophilus. It seems implausible given the divergence of the two proteins and the great conservation of IPO7 in the enveloping exon -- what selective force could maintain this if an auxillary gene is available to take on the nuclear import role?

                                                            ^                                                                       ^             

IPO7_homSap  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ      
IPO7_panTro  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_ponAbe  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_rheMac  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_calJac  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_tarSyr  EELGSDEDDIDEDGQEYLEILAKQAGEDGDEEEWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   ................................E.....................E..............      
IPO7_micMur  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_tupBel  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_musMus  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_ratNor  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_dipOrd  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_cavPor  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_speTri  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDDDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   ...............................D.....................................      
IPO7_oryCun  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_ochPri  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_vicPac  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_turTru  EELGSDEDDIDVDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   ...........V.........................................................      
IPO7_bosTau  EELGSDEDDIDEDGQEYLEILAKQAGEDGDEEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   ..............................E......................................      
IPO7_equCab  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_canFam  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_myoLuc  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDDEWEENDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   ...............................DE...N................................      
IPO7_pteVam  EELGSDEDDIDEDGQEYLEILAKQA-EDGDDEDWR-DDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .........................-........R-.................................      
IPO7_eriEur  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_sorAra  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_loxAfr  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_proCap  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_echTel  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_dasNov  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_choHof  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_monDom  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ   ................................E.....................E..............      
IPO7_sarHar1 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ   ................................E.....................E..............      
IPO7_sarHar2 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYFTIIDDEENPVDEYQIFKAIFQ   ................................E..............F......E..............      
IPO7_ornAna  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ   .....................................................................      
IPO7_galGal  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKTIFQ   .................................................................T...      
IPO7_taeGut  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPIDEYQIFKTIFQ   .........................................................I.......T...      
IPO7_anoCar  EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPIDEYQIFKAIFQ   .........................................................I...........      
IPO7_xenTro  AELGSDEDDIDEEGQEYLEILAKQAGEDGDDEDWEDDDAEETALEGYTTLIDDEDTPIDEYQIFKAIFQ   A...........E......................D...........T.L.....T.I...........      
IPO7_tetNig  AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDEDWEEDDAEETALEGYTTTVDDEDNFVDEYQIFKAILQ   A...........E......M...........................T.TV.....F..........L.      
IPO7_takRub  AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDEDWEDDDAEETALEGYTTNIDDEDNFVDEYQIFKAILQ   A...........E......M...............D...........T.N......F..........L.      
IPO7_gasAcu  AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDEDWEEDDAEETALEGYTTAVDDEDNLVDEYQIFKAILQ   A...........E......M...........................T.AV.....L..........L.      
IPO7_oryLat  AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDDDWEEDDAEETALEGYTTAIDDEDNFVDEYQIFKAVLQ   A...........E......M...........D...............T.A......F.........VL.      
IPO7_danRer  AELGSDEDDIDDEGQEYLEMLAKQAGEDGDDEDWEEDDAEETALEGYTTLVDDEDNLVDEYQIFKAIMQ   A..........DE......M...........................T.LV.....L..........M.      
                                                            ^                                                                       ^           
                                                            ^
IPO8_hg18_23 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI
IPO8_panTro2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI
IPO8_gorGor1 -EISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI
IPO8_ponAbe2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI
IPO8_rheMac2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI
IPO8_calJac1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDEDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI
IPO8_tarSyr1 EEISSDEEETTVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPIDLDHSVDEYQFFTQALL
IPO8_micMur1 -EIASDEEEMNVNAQAMQSSNGRGEDEEEDDDDWDDEVVEETALEGFSTPLDLDSSVDEYQFFTQALL
IPO8_otoGar1 KEISSDEEESNVKAQAMQSNNGRGDDEEEEEDDWDEEVLEETALEGFSTPLDLDSSVDEYQFFTQALL
IPO8_dipOrd1 EEISSDEEEKSVSVQAMQSVNRRGADEEDEDEDWEEEILEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_cavPor3 EEISSDEEETNANAQAMQSNTRKG--EEEEDDDWDEEVLEETALEGFSTPLDLDDSVDEYQFFTQALL
IPO8_speTri1 EEISSDEEDTNITAQAMQANNGRSGDEEEEQDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_oryCun1 EEISSDEEETNVASQAVQSSSGRGEDEEEDDDDWADEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_ochPri2 -EISSDEEETNPSTQAMQSSTGRGEDEDEEEEEWDDEVLEETALESFSTP----ECVDEYQFFTQALL
IPO8_vicPac1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_turTru1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_bosTau4 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_equCab2 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_felCat3 EEISSDEEETNVTAQAMQSNNGRGEDEEEEEDDWDEEVLEETALEGFSTPLDLDNSVDEYQIFTQALL
IPO8_canFam2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_myoLuc1 EEISSDEEEANITAQAMQSKNGRGEEEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL
IPO8_pteVam1 E-ISSDEE-ANVTAQAMQPNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYLFFTQALL
IPO8_eriEur1 EEISSDEEETTVGVQAKQPSNGRVEAEEDDDDDWEEELLEETTLEGFSTPLDLDGSVDEYQFFTQALL
IPO8_loxAfr2 -EISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDSSVDEYQFFTQALL
IPO8_proCap1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDSSVDEYQFFTQALL
IPO8_echTel1 EEISSDEEETNVTAQAMQSTNGRGDNEEEEEDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFAQALL
IPO8_choHof1 EEISSDEEETSVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDSNVDEYQFFTQALL
IPO8_monDom4 EEIPSDEEDTNEARQAL--S-GGGEDEEEDDDDWDEEVLEETALEGFSTPLDLDDGVDEYQFFTQALL
IPO8_ornAna1 EEIPSDEEETNETGQLMQENLGGDEEEDDEDDDWDEDVLEETALEGFSTPLDLENSVDEYQFFTQALL
IPO8_galGal3 EEIPSDEEETNEVSQAMQENHGEEEDDDDDDDDWDEDALEETALEGFSTPLDLENGVDEYQFFTQALL
IPO8_taeGut1 EEIPSDEDETNEVSQAMQENHGEEEDEDDDDDDWDEDALEETALEGFSTPLDLENGVDEYQFFTQALL
IPO8_anoCar1 EEIPSDEEEANEVTQEMQENHVGDEDDDDDDDDWDDDALEETALEGFSTPIDLEDAVDEYQFFTQALI
IPO8_xenTro2 EEIASDEEEAN---QAMQQN---GEDAEEEDEDWDDEVLEETALEGFSTPLDCEDALDEYQFFTNALL
IPO8_tetNig1 QEIPSDEDEVNENH-A-QQASRNGAEDEEEDDYWEDDCFEGTALEEYTTPLDFDNGEDEYLFFTSTLL
IPO8_fr2_23_ QEIPSDEDEVSENHSA-PLPNMSGEDDEEEDDYWDDDGFEGTPLEEYSTPLDFENGEDEFHFFTSTLL
IPO8_gasAcu1 QEIPSDEDEVTENRKAVQHANR-EEEEEDDEDDWDNDCFEGTPLEEYSTPLDYDNGEDEYQFFASALL
IPO8_oryLat2 EEIPSDEDEVNENREAVQHHSR-EDDDDDEEDYWEEDGFEGTPLEEYSTSLDYDNGEDEYEFFTCALL
IPO8_danRer5 EEIPSDEDEVGEKGVAIRRSHREDDDDEDDDEYWDDEGLEGTPLEEYSTPLDCDNGEDEYQFFTASLL
                                                            ^                                                                      ^           
>IPO7_homSap
MDPNTIIEALRGTMDPALREAAERQLNE
AHKSLNFVSTLLQITMSEQLDLPVRQA
GVIYLKNMITQYWPDRETAPGDISPYTIPEEDRHCIRENIVEAIIHSPELIR
VQLTTCIHHIIKHDYPSRWTAIVDKIGFYLQSDNSACWLGILLCLYQLVKNYE
YKKPEERSPLVAAMQHFLPVLKDRFIQLLSDQSDQSVLIQKQIFKIFYALVQ
YTLPLELINQQNLTEWIEILKTVVNRDVPN
ETLQVEEDDRPELPWWKCKKWALHILARLFER
YGSPGNVSKEYNEFAEVFLKAFAVGVQQ
VLLKVLYQYKEKQYMAPRVLQQTLNYINQGVSHALTWKNLKPHIQ
GIIQDVIFPLMCYTDADEELWQEDPYEYIRMKF
DVFEDFISPTTAAQTLLFTACSKRKE
VLQKTMGFCYQILTEPNADPRKKDGALHMIGSLAEILLK
KKIYKDQMEYMLQNHVFPLFSSELGYMRAR
ACWVLHYFCEVKFKSDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEK
AKEYITPFIRPVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHL
AMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKE
ITQQLEGICLQVIGTVLQQHVL
EFYEEIFSLAHSLTCQQVSPQMWQLLPLVFEVFQQDGFDYFT
DMMPLLHNYVTVDTDTLLSDTKYLEMIYSMCKK
VLTGVAGEDAECHAAKLLEVIILQCKGRGIDQ
CIPLFVEAALERLTREVKTSELRTMCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLG
1 LHDRKMCVLGLCALIDMEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDDDDEAEDDDET 1
2 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ 1
2 TIQNRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAH 1
ESKMIEKHGGYKFSAPVVPSSFNFGGPAPGMN*

>IPO7_monDom
MDPNTIIEALRGTMDPALREAAERQLNE
AHKSVNFVSTLLQITMSEQLDLPVRQA
GVIYLKNMITQYWPDRETTPGEIPPYTIPEEDRHCIRENIVEAIIHSPELIR
VQLTTCIHHIIKHDYPSRWTAVVDKIGFYLQSENSACWLGILLCLYQLVKNYE
YKKPEERSPLVAAMQHFLPVLKDRFIQLLPDQSDQSVLIQKQIFKIFYALVQ
YTLPLELINQANLTEWIEILKTVVNRDVPP
ETLQVEEDDRPELPWWKCKKWALHILARLFER
YGSPGNVSKEYNEFAEVFLKAFAVGVQQ
VLLKVLYQYKEKQYMAPRVLQQTLNYINQGVSHAVTWKNLKPHIQ
GIIQDVIFPLMCYTDADEELWQEDPYEYIRMKF
DVFEDFISPTTAAQTLLFTACSKRKE
VLQKTMGFCYQILTEPNADPRKKDGALHMIGSLAEILLK
KKIYKDQMEYMLQNHVFPLFSSDLGYMRAR
ACWVLHYFCEVKFKSDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEK
AKEYITPFIRPVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHL
AMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKE
ITQQLEGICLQVIGTVLQQHVL
EFYEEIFSLAHSLTCQQVSPQMWQLLPLVFEVFQQDGFDYFT
DMMPLLHNYVTVDTDTLLSDTKYLEMIYSMCKK
VLTGVAGEDAECHAAKLLEVIILQCKGRGIDQ
CIPLFVEAALERLTREVKTSELRTMCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLG
LHDRKMCVLGLCALIDLEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDEDDEADDDEET
EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ
AIQSRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAH
ESKMIEKHGGYKFNAPVVPSSFNFGGPAPGMN*

>IPO7_sarHar
LHDRKMCVLGLCALIDLEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDEDDEADDDEET
EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ
AIQSRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAH

>IPO8_sarHar
EEIPSDEEDTNETSQTMHENNGGGDEDEEEDDDWDEDVLEETALEGFSTPLDLEDS-VDEYQFF

Case of PPFIA3

chr4_22002 PPFIA3 15  'anomalous mapping from monDom5 to human'
>contig00001  length=298   numreads=4
LIQEEKETTEQRAEELESRVSGSGLDSLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNTAEKP
........................................................F..................G.V.
                                                        ^
 56 F=2(43) S=2(37)

Here both individuals differed from Monodelphis by S->F at position 56 of PPFIA3 with a confusing end to the exon.

Pseudogene issues: Not applicable.

Paralog issues: PPFIA3 (liprin) has 3 paralogs with considerable (but readily differentiable) sequence identity in this exon. These latter genes are more similar to each other than to PPFIA3, yet all 4 have S at the position occupied by F in tasmanian devil. The ancestral gene duplications must be quite old because lamprey has at least two copies and PPFIA3 itself is readily traced to shark.

PPFIA3 is missing in chicken and finch (proving it is not an essential gene in vertebrates) though present in lizard and frog. These latter species have a one residue mid-exon insert relative to mammals compensated for well past the key residue with a one residue deletion. All three species of marsupials with available data have an 8 residue insert three residues from the end of the exon (which still ends in phase 0 like all other orthologs). These indels have seriously affected the UCSC 44-species alignment quality. The batch of sequences immediately below are hand-curated directly from trace reads but otherwise are provided 'as is.'

The S-->F changed observed in tasmanian devil is likely very significant to protein function given the immense conservation of this residue and its flanking environment. However given the numerous independent indels still within this exon -- especially the 8 residue insert in the marsupial stem -- it would be difficult to argue that S-->F could not somehow be compensated with material impact on function. The complete loss of the gene in two birds (together these species have overwhelming trace coverage and many transcripts) establishes either that PPFIA3 lost its importance important or that one of its three paralogs can assume its function.

No structural data relevent to this exon exists at PDB. The entry at SwissProt shows two predicted phosphoserines within the exon but not at the serine here. Predicted domains and secondary structure coils are not applicable to this exon either. The function is somewhat understood: it may regulate the disassembly of focal adhesions, localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane and forms homodimers and heterodimers with other liprins.

                                  p                             *       p
homSap LIQEEKETTEQRAEELESRVSSSGLD-SLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGT--------DKA
sarHar LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRASCSLPPSLTTSTLASPSPPSSGHFTPRPAPPSPAREAPANSTGNVADKP
monDom LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNTAEKP
macEug LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNAADKP
ornAna LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRGGSALPASLTSSTLASPSPPSSGHSTPRLAPPSPAREGS--------EKT
anoCar LIQEEKESTEQRAEEIESRVTSASLDGSLGRYRSGASIPPSVTSSTLASPSPPSSGHSTPRLAPHSPARDG---------EKM
xenTro LIQEEKETTELRAEEIESRVTSGTLDGSLGRYRSASSIPTSVTTSTLASPSPPSSGHSTPRITPHSPAREG---------DKF  PPFIA3

monDom LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTL      PPFIA2
monDom LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSLSSLPPHPSSCLSG--SSPPGSGRSTPRRHPHSPAREVDRLGIMTL      PPFIA1
monDom MIQEEKESTELRAEEIETRVTSGSMEALNLQLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL      PPFIA4

Homoplasy (recurrent mutation) issues:

Known variations:

Side issues:

                                                        *


>PPFIA3_sarHar FHGHM9L01BYK1T length=435 xy=0686_3455 region=1 
aggcttatccaggaggaaaaggagaccacggagcagcgggccgaggaactggagagccgc
    L  I  Q  E  E  K  E  T  T  E  Q  R  A  E  E  L  E  S  R 
gtgtccggctctggcctggactccctgggacgctaccgggccagctgctccctcccgcct
 V  S  G  S  G  L  D  S  L  G  R  Y  R  A  S  C  S  L  P  P 
tccctgaccacgtccaccctggctagcccttccccccccagctctgggcactccacgccc
 S  L  T  T  S  T  L  A  S  P  S  P  P  S  S  G  H  S  T  P 
cgccctgctccccccagtcccgcccgggaagccccggccaacagcaccggcaacgtggca
 R  P  A  P  P  S  P  A  R  E  A  P  A  N  S  T  G  N  V  A 
gataagcccgtgagt
 D  K  P   

>PPFIA3_monDom phase 0
ggccactccacccctcgccctgccccgcccagccctgctcgggaagctccagccaacagcactagcaacactgcagaaaagcctgtgagt
 G  H  S  T  P  R  P  A  P  P  S  P  A  R  E  A  P  A  N  S  T  S  N  T  A  E  K  P  V  S  

>PPFIA3_macEug Macropus eugenii phase 0, assembly has early frameshift due to extra G
aggctcatccaggaggagaaggagacgacggaacagcgggcagaggagctggagagccgg
 R  L  I  Q  E  E  K  E  T  T  E  Q  R  A  E  E  L  E  S  R 
gtgtctggctctggcctggactccttgggacgctaccgggccagctgctcccttccacct
 V  S  G  S  G  L  D  S  L  G  R  Y  R  A  S  C  S  L  P  P 
tccctgactacatccaccctggccagcccttcaccccccagctctggtcactccacaccc
 S  L  T  T  S  T  L  A  S  P  S  P  P  S  S  G  H  S  T  P 
cgccctgccccacccagccctgcccgagaagccccagccaacagcactagcaacgctgca
 R  P  A  P  P  S  P  A  R  E  A  P  A  N  S  T  S  N  A  A 
gataagcctgtgagt
 D  K  P  V  S  

>PPFIA3_xenTro
aggttaatccaagaggaaaaggagacaacagagttgcgggctgaagaaatagagagtcga
    L  I  Q  E  E  K  E  T  T  E  L  R  A  E  E  I  E  S  R 
gtgaccagcggcactctggacggatcactgggacgctaccgttctgccagttccatcccc
 V  T  S  G  T  L  D  G  S  L  G  R  Y  R  S  A  S  S  I  P 
acctccgtcaccacatcaactctagccagtccctcaccacccagcagtgggcattccacc
 T  S  V  T  T  S  T  L  A  S  P  S  P  P  S  S  G  H  S  T 
ccgcgcatcacgccacacagccctgccagagaaggagacaaatttgtaagttcctttcaa
 P  R  I  T  P  H  S  P  A  R  E  G  D  K  F  V  S  

>PPFIA3_ornAna platypus phase 0
ctgatccaggaggaaaaggagacgacagagcagcgggccgaggagctggagagccgggtg
 L  I  Q  E  E  K  E  T  T  E  Q  R  A  E  E  L  E  S  R  V 
tccggctcggggttggactccctgggccggtaccggggcggcagtgccctgcccgcctcc
 S  G  S  G  L  D  S  L  G  R  Y  R  G  G  S  A  L  P  A  S 
ctcacctcctccaccctggccagcccctctccccccagcagcggccactccaccccccgc
 L  T  S  S  T  L  A  S  P  S  P  P  S  S  G  H  S  T  P  R 
ctggcgccccccagccccgcccgcgaggggtccgaaaaaaccgtaagtggaaaaggccgc
 L  A  P  P  S  P  A  R  E  G  S  E  K  T   
 
>PPFIA3_anoCar
aggttgatccaggaggaaaaagaatccacagaacaacgggcagaggaaatcgagagccga
    L  I  Q  E  E  K  E  S  T  E  Q  R  A  E  E  I  E  S  R 
gtgactagtgccagcttggacggttccctcggccgctaccgctcaggcgcttccatccct
 V  T  S  A  S  L  D  G  S  L  G  R  Y  R  S  G  A  S  I  P 
ccctccgtcaccagctccaccctggccagcccttctccccccagcagtggccactccacc
 P  S  V  T  S  S  T  L  A  S  P  S  P  P  S  S  G  H  S  T 
ccccgcttggcgccccatagccctgctcgcgatggggaaaaaatggtatgtcatgactgt
 P  R  L  A  P  H  S  P  A  R  D  G  E  K  M   

>PPFIA3_homSap phase 0
aggctgatccaagaggagaaggagacaacagaacagagggcagaggagctggagagtcgg
 R  L  I  Q  E  E  K  E  T  T  E  Q  R  A  E  E  L  E  S  R 
gtgtccagctctggcttggactcgttgggccgctaccgcagcagctgctccctgcccccc
 V  S  S  S  G  L  D  S  L  G  R  Y  R  S  S  C  S  L  P  P 
tccctcaccacctctacccttgccagcccctcccctcccagctctggccactcaacaccc
 S  L  T  T  S  T  L  A  S  P  S  P  P  S  S  G  H  S  T  P 
cgcctggcaccccctagccctgcccgtgagggcaccgacaaggctgtgagtgctctgaag
 R  L  A  P  P  S  P  A  R  E  G  T  D  K  A  V  S  A  L  K 
tctccccagcctagt
 S  P  Q  P  S 

>PPFIA3_calMil Callorhinchus milii
MIQEEKETNELRAEEIESRVGSGTLEGPQGGGYRSAASLSHSVTASTLASPSPPNSGHSPRMAPHSPAREGDRVGIGNTVS
atggcacctcacagcccagccagggagggggacagggtcggcatcggcaacacagtgagt
 M  A  P  H  S  P  A  R  E  G  D  R  V  G  I  G  N  T  V  S 


PPFIA3_homSap  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_panTro  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_rheMac  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_calJac  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_tarSyr  LIQEEKDTTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT
PPFIA3_micMur  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_mm9_15_ LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT
PPFIA3_rn4_15_ LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPARE-TDKT del verified
PPFIA3_dipOrd  LIQEEKETTEQRAEELESRVSSSGLDSLSRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_cavPor  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_ochPri  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_turTru  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_bosTau  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_equCab  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_canFam  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_sorAra  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT
PPFIA3_loxAfr  LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA
PPFIA3_proCap  LIQZEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT
PPFIA3_monDom  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNTAEKP
PPFIA3_ornAna  LIQEEKETTEQRAEELESRVSGSGLDSLGRYRGGSALPASLTSSTLASPSPPSSGHSTPRLAPPSPAREGSEKT
PPFIA3_anoCar  LIQEEKESTEQRAEEIESRVTSASLGSLGRYRSGASIPPSVTSSTLASPSPPSSGHSTPRLAPHSPARDGEKM
PPFIA3_galGal  missing
PPFIA3_taeGut  missing
PPFIA3_xenTro  LIQEEKETTELRAEEIESRVTSGTLGSLGRYRSASSIPTSVTTSTLASPSPPSSGHSTPRITPHSPARE--DKF
PPFIA3_tetNig  LIQEEKENTELRAEEIENR--SVALATLGRDAAGRFLPSSITSSTLASPSPPSSGHSTPRL-PHSPAREPSDR-
PPFIA3_fr2_15  LIQEEKESTELRAGEIESRVSSVALASLGRDSIGRYMTPSITSSTLASPSPPSSGHSTPRL-PHSPARETTDR-
PPFIA3_gasAcu  LIQEEKENTELRAEEIESRVSSVALASLGGDSVGRYMTPSITSSTLASPSPPSSGHSTPRL-PHSPARETTDR-
PPFIA3_oryLat  LIQEEKENTELRAEEIESR--SVALASLGRDSAGRFIPSSITSSTLASPSPPSSGTSTPRL-PHSPAREMTDR-
PPFIA3_danRer  LIQEEKESTELRAEEIESRVSSVALASLGRDSTGRFIPPSLTSSTLASPSPPSSGHSTPRL-PHSPARETTDR-

PPFIA2_homSap  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_panTro  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_ponAbe  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_rheMac  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_calJac  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_tarSyr  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_micMur  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_otoGar  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_tupBel  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_mm9_16  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_rn4_16  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_cavPor  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_speTri  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_oryCun  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHQGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_vicPac  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_turTru  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_bosTau  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_equCab  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_felCat  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_canFam  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTTITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_pteVam  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_eriEur  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_sorAra  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_proCap  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_echTel  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_dasNov  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_choHof  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_monDom  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTL
PPFIA2_ornAna  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_galGal  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHQGTSITGSVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_taeGut  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHQGTSITGSVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_anoCar  LIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASITASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTL
PPFIA2_xenTro  LIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL
PPFIA2_tetNig  LIQEEKESTELRAEEIEHRVASVSLEGLNLARIHHGASITASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL
PPFIA2_fr2_16  LIQEEKESTELRAEEIENRVASVSLEGLNLARIHHGASITASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL
PPFIA2_gasAcu  LIQEEKESTELRAEEIENRVASVSLEGLNLARIHHGASITASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL
PPFIA2_oryLat  LIQEEKESTELRAEEIENRVASVSLEGLNLARIHHGVSMTASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL
PPFIA2_danRer  LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASATASSLASSSPPSGHSTPKLTPRSPARDMERMGVMTL

PPFIA1_homSap  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_panTro  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_gorGor  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_ponAbe  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_rheMac  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_calJac  LIQEEKENTEQRAEEIENRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPSSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_tarSyr  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRPVSSIPPCPASSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_micMur  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLGGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_otoGar  LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_tupBel  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRMPHSPAREVDRLGIMTL
PPFIA1_mm9_15_ LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTL
PPFIA1_rn4_15_ LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTL
PPFIA1_dipOrd  LIQEEKESTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRVPHSPAREVDRLGVMTL
PPFIA1_cavPor  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRMVPHSPAREVDRLGVMTL
PPFIA1_speTri  -------------EEIESRVGSGSLDNLGRFRSMSSLPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTL
PPFIA1_turTru  LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSVSSIPPYPASSRASSSPPSSGRPTPRRAPHSPAREVDRLGVMTL
PPFIA1_bosTau  LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSMSSIPPYPASSLAGSSPPSSGRSTPRRMPHSPAREVDRLGIMTL
PPFIA1_equCab  LIQEEKENTEQRAEEIESRVGSGSFGNLR-FRSVSSIPLYPASSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_felCat  LIQEEKESAEQRAEEIESRVGSVFLDSPGRFRPAGSGAPHPASPLAGPSPPHSGRSTPRRGPHSPAREVDRLGVMTL
PPFIA1_canFam  LIQEEKESTEQRAEEIESRVGSGSLDSPGRFRSLGDAPPHPTSVLTGPSPPHSGRSTPRRGPHSPAREVDRLGVMTL
PPFIA1_myoLuc  LIQEEKESTEQRAEEIESRVGSGSLDNLGRFRSMSS--PYPGSSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_pteVam  LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSMSAIPPYPASSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_sorAra  LIQEEKESTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_loxAfr  LIQEEKESAEQRAEEIESRVGSGSLDNLDRFRSMSSIPPYPAPSLAGSSPPGSGRSTPRRIPQSPAREVDRLGIMTL
PPFIA1_proCap  LIQEEKESAEQRAEEIESRVGSGSLDNLDRFRSVSSIPPYPASSLAGSSPPGSGRSTPRRIPQSPAREVDRLGIMTL
PPFIA1_echTel  LIQEEKENAEQRAEEIESRVGSGSLSDLGHFRPLGSVPPHPSSALAGSSPPGSGRSTPRRIPQSPSREVDQLGIMTL
PPFIA1_dasNov  LIQEEKENTEQRAEEIESRVGSGTLDNLGRFRSLSSIPPYPASSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_choHof  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSLSAIPPYPASSLASSSPPGSGRSTPRRMPHSPAREVDRLGVMTL
PPFIA1_monDom  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSLSSLPPHPSSCLSGSSPPGSGRSTPRRHPHSPAREVDRLGIMTL
PPFIA1_ornAna  LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPGSSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL
PPFIA1_galGal  LIQEEKENTEQRAEEIESRVGSGSLDAHGRFRSMSSIPPPYGGSLAGSSPPGSGRSTPRRIPHSPTREVDRLGIMTL
PPFIA1_taeGut  LIQEEKENTEQRAEEIESRVGSGSLEAHGRFRSLGSIAPALGGALAGSSPPGSGRSTPRRIPHSPAREVDKLGIMTL
PPFIA1_anoCar  LIQEEKENTEQRAEEIESRVGSGSLENLGRFRSMSSLPAPFRGSLSGTSPPGSGRSTPRRMPHSPAREVDRLGIMTL
PPFIA1_xenTro  LIQEEKETTEQRAEEIESRVGSGSLDNLGRFRSITSIPPFTGTSLAGSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA1_tetNig  MIQEEKESTAIRAEEIECRVGSEGLG--GRFRSMSSIPPCMGSSLGG-SPPGSGHSTPRRIPCSPNRELDRMGVMTL
PPFIA1_fr2_15  MIQEEKESTAIRAEEIECRVGSDGLG--GRFRSMSSIPPCMGSSVGG-SPPGSGHSTPRRIPRSPNRELDRMGVMTL
PPFIA1_gasAcu  MIQEEKENTVIRAEEIECRVGSDSLG--GRFRSMGSIPPCPGSSLGG-SPPGSGHSTPRRVPRSPNRELDRMGVMTL
PPFIA1_oryLat  MIQEEKESTAIRAEEIECRVGSDSIG--GRFRSLSSIPPCAGSSLGG-SPPSSGHSTPRRIPRSPNRELDRMGVMTL
PPFIA1_danRer  LIQEEKESTELRAEEIENRVASVSLE--GRIWHESTIPPSTASSLAS-SSPPSGHSTPKLTPRSPARDMERMGVMTL
PPFIA1_petMar  LIQEEKESTEQLAEEIEIRVGGSSGGGGGRLRSARSIPGSATATLATNSAPVSGYATPKRLTHSPAHDPDRHGAMTL

PPFIA4_homSap  MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_gorGor  MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTAKLTSRSAAQDLDRMGVMTL
PPFIA4_ponAbe  MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_rheMac  MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_calJac  MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASTSPPLSGRSTPKLTSRSAAQGLDRMGVM--
PPFIA4_micMur  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_tupBel  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_musMus  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_ratNor  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_dipOrd  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPSLSGRSTPKLTSRSPAQDLDRMGVMTL
PPFIA4_cavPor  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGIMTL
PPFIA4_ochPri  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSTAQDLDRMGVMTL
PPFIA4_vicPac  M-QEEKESTELRA-EIDTEVTSGSLEVLKLXLKLQCGGI------------SPPLSGRSAPKLTSRSAAQDLDRMGVMTL
PPFIA4_turTru  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPFSGRSTPKLTSRSATQDLDRMGVMTL
PPFIA4_bosTau  MIQEEKESTELRAEELETRVTSGSMEALDLTQLHKRGSIPTSLTALSLASASPPLSGRATPKLTSRSAAQDLDRMGVMTL
PPFIA4_equCab  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_felCat  MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_canFam  MIQEEKESTELRAEEIETRVSSGSVEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLASRSAAQDLDRMGVMTL
PPFIA4_myoLuc  MIQEEKESTELRAEEIETRVTSGSMEALNLTQPHRRGPIPTSLTALSLASGSPAFSGRSTAKCASRSAVQDLDRMGVMTL
PPFIA4_eriEur  MIQEEKESTELRAEENETRVTSGSMEALNLSQRRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_sorAra  MILEEKEATELRAEEIETRMNSASIE-LDSSQLRKRASITTPZMPLSLARASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_loxAfr  MIQEEKESTELRAEEIETQVTSGSMEALNL-QLRKRASIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_proCap  MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRASIPTSLTALSLASTSPQLSGRSTPKLTSRSTAQDLDRMGVMTL
PPFIA4_echTel  MIQEEKESAELRAEEIETRVTSGSMEALNL-QLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_dasNov  MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_choHof  MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_monDom  MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL
PPFIA4_ornAna  LIQEEKESTELRAEEIENRVASVSLEGLNL-RVHPGTSITASVTASSLASSSP--SGHSTPKLTPRSPAREMDRMGVMTL
PPFIA4_galGal  MIQEEKESTELRAEELETRVTSGSMEGLNL-QLCKRASIPTSLTALSLASSSPPLSGRSTPKLTSRSAAQDLDRMGIMTL
PPFIA4_taeGut  MIQEEKESTELRAEELETRVTSGSMEGLNL-QLCKRASIPTSLTALSLASSSPPLSGRSTPKLSSRSAAQDLDRMGIMTL
PPFIA4_anoCar  MIQEEKESTELRAEQLESRVTSGSMEALNL-QLRKRASIPTSLTALSLASSSPPISGRSTPKLSSRSAAQDLDRICSMTL
PPFIA4_xenTro  LIQEEKETTEQRAEEIESRVGSGSLDNLG---FQVHHFNSPFZVV-SLAGSSPPGSGRSTPRRIPHSPAREVDRLGVMTL
PPFIA4_tetNig  LIQEEKESTELRAEEIEHRVASVSLEGLNL--PPPRR--PASATASSLASSSP--SGHSTPKLDPRSPARDMERMGVMTL
PPFIA4_takRub  MIQVERESADLRSDEIESRVNSGSMDGLNV--LRPRA--PTSATAQSLASSCSPHSGHSTPKHHSRNAGHH---LGIMTL
PPFIA4_gasAcu  MIQVERESADLRSDEIESRVNSGSMDGLNV--LRPRA--PTSATAQSLASSSSPPSGHSTPKHHSRNASHH---LGIMTL
PPFIA4_oryLat  MIQVERESADLRSGDIESRVNSGSMDGLNV--LRPRA--PTSATAQSLASSSSPHSGHSTPKHHGRNASHH---LGIMTL
PPFIA4_danRer  MIQVERESAELRADEIESRVNSGSMDGLNV--LRPRSSIPTSVTALSLASSSP--SGRSTPKLTSGSTAHE---LGIMTL
PPFIA4_petMar  LIQ-EKESTEQRAEEIESRVGSGSLDSLSL-QQRDGGSLPVSLTGSSLASSSPPVSGRSTPKFTPRSPARDADRAGA---

Case of WDFY3

chr5_2532 WDFY3 19
>contig00001  length=482   numreads=8
DDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK
................T..............................T..L.....N...
                ^
16      T=3(117)        A=5(138)

Tasmanian devil differs from Monodelphis by A->T at position 16. The variation observed later in the exon
is largely in opossum rather than other marsupials and occurs at positions with tight reduced alphabets.

Pseudogene issues: None.

Paralog issues: Some weak paralogs exist in human. These are poorly conserved in the region in question even about the key residue. While this weakens the overall invariance of the key residue, it also eliminates any possibility of cross-alignment to inappropriate homologs.

WDFY3	0	 WD repeat and FYVE domain containing 3 isoform
WDFY4	0	 WDFY family member 4
LYST	3e-114	 lysosomal trafficking regulator
NBEAL1	6e-109	 neurobeachin-like 1 isoform 1
NBEAL2	7e-109	 neurobeachin-like 2
LRBA	5e-100	 LPS-responsive vesicle trafficking, beach and
NBEA	3e-98	 eurobeachin
NSMAF	1e-78	 neutral sphingomyelinase (N-SMase) activation

Homoplasy (recurrent mutation) issues: None.

Known variations: Not a known disease gene; no relevent human variants known.

Side issues: None.

Structural significance: WDFY3 encodes a very large peripheral membrane protein of 3526 aa and 65 codinbg exons containing two leucine-rich repeats, a BEACH doman, five WD domains, a FYVE-domain,3 phosphotyrosines, 2 phosphoserine, and 1 phosphothreonine. However none of these are immediately relevent to the three exons centered on the SNP-containing exon. SuperFamily identifies the key exon significant matches (4e-09) as a concanavalin A-like lectin/glucanase domain. It co-localizes with autophagic structures in starved cells. The few transcripts that cover this region arise from testes (Xenopus), heart (chicken), early embryo (pig), and colon and hypothalamus (human), not informative as to function.

Functional significance: The substitution of threonine for alanine in proteins in general has quite mild effects. Alanine is the most generic amino acid and never catalytically active; threonine is polar but not charged and only somewhat bulkier. However the comparative genomics of this alanine in WDFY3 says this alanine is very different -- it is completely invariant over immense branch length back to chondrichthyes with the sole exception of Sarcophilus.

The embedding exon has more nearby variability than some of the other candidates. Its rather diverged paralog WDFY4 has leucine in place of alanine; this leucine is quite well conserved but has some exceptions. Note the alanine is not one of the better conserved residue patches in the overall region. Thus it appears that the substitution A-->T will have a significant effect on function but not a catastrophic one on core properties.

WDFY3 VSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVKLHYVHSTPG
      VST+E+  Q  +D    E        C  + RCG+L+  GQWHHL +V++K M ++ T +  +DGQ++ + K+ Y+ + PG 
WDFY4 VSTEEKEFQP-LDVMEPEDDSEPSAGCQLQVRCGQLLACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAKMLYIQALPG
                        *                                                             *                                                
homSap VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK homSap
panTro VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. panTro
gorGor VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. gorGor
ponPyg VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. ponPyg
macMul VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. macMul
calJac VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. calJac
otoGar VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK .......................D..V.................................. otoGar
musMus VDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYLDGQLVSTVK .........................VV.......A.L..R...........L.....S... musMus
ratNor VDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYIDGQLVSTVK .........................VV.......A.L..R.................S... ratNor
dipOrd VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYLDGQLVSTVK ..........................V........................L.....S... dipOrd
cavPor VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLALVMSKGMLKNSTATLYIDGQLVSTVK ................................................T........S... vicPac
speTri VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. speTri
ochPri VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK ..........................V..............................S... ochPri
vicPac VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTATLYIDGQLVSTVK ..........................V.....................T........S... bosTau
turTru VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSRGMLKNSTAALYIDGQLVSTVK .......................................R.................S... turTru
bosTau VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTATLYIDGQLVSTVK .......................D..V........................L......I.. taeGut
equCab VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. equCab
felCat VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. felCat
canFam VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. canFam
echTel VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. echTel
dasNov VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQPVTTVK ..........................V............................P.T... dasNov
choHof VDDFSEESSFYEILPCCAHFRCGELIVEGQWHHLVLVMSRGMLKNSTAALYIDGQLVNTVK ..........................V.......A.............T........S... cavPor
monDom VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK .......................D..V..............................S... monDom
macEug VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYLDGQLVNTVK .......................D..V........................L......... macEug
sarHa1 VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTATLYLDGQLVNTVK .......................D..V.....................T..L......... sarHar
sarHa2 VDDFSEESSFYEILPCCTRFRCGDLIVEGQWHHLVLVMSKGMLKNSTATLYLDGQLVNTVK .................T.....D..V.....................T..L......... sarHar
ornAna ADDFSEESSFYELLPCCAHFRCGDLIAEGQWHHLVLVMSKGMLKNSTATLYIDGQLVNTVK A...........L.....H....D..A.....................T............ ornAna
galGal VDDFSEESSFYEILPCCARFRCGELIAEGQWHHLVLVMSKGMLKNSTAALYLDGQLVNTVK ..........................A........................L......... galGal
taeGut VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYLDGQLVNIVK ..................H.......V............R..................... choHof
anoCar VDDFGEESSCYEILPCCARFRCGDHIVEGQWHHMVLVMSKGMLKNSTAALYIDGQLINTVK ....G....C.............DH.V......M......................I.... anoCar
xenTro VDDFSEEASFYEILPCCARFRCSDLIMEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK .......A..............SD..M..............................S... xenTro
tetNig SDESSEEASFYEILPCCARFRCGEAIAEGQWHHLVLVMSKGMLKNSMATLYIDGQLINTVK S.ES...A................A.A...................M.T.......I.... tetNig
takRub SDESSEEASFYEILPCCARFRCGEVIAEGQWHHLVLVMSKGMLKNSMATLYLDGQLINTVK S.ES...A................V.A...................M.T..L....I.... takRub
gasAcu SDDSREDSFFYEILPCCARFRCGELIAEGQWQHLVLVMSKGMLKNSMATLYLDGQLVNTVK S..SR.D.F.................A....Q..............M.T..L......... gasAcu
oryLap SDESSEEASFYEILPCCARFRCADLIAEGQWHHLVLVMSKGMLKNSMATLYIDGQLVNTVK S.ES...A..............AD..A...................M.T............ oryLap
danRer VDDFSEESSFYEILPCCARFRCADLITEGQWHHLLLVMSKGMLKNSMATLYIDGQMVSTVK ......................AD..T.......L...........M.T......M.S... danRer
calMil VDDFSEESSFYEILPCCARFRCTDLINEGQWHHLVLVMSKGMLKNSTATLYVDGQHVNTVK ......................TD..N.....................T..V...H..... calMil
                        *                                                             *                                                
Less conseervation of this position in paralog WDFY4:

WDFY4_hg18_18 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK
WDFY4_panTro2 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK
WDFY4_gorGor1 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK
WDFY4_ponAbe2 DVMEPEDDSEPSAGCQLQVRCGQ L LTCGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK
WDFY4_rheMac2 DVMEPEDDSEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK
WDFY4_calJac1 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLNGQVIGSAK
WDFY4_micMur1 DVMEPEDDSEPSGGRQLLVRWSQ L LTWGQGHHLGGVVTKEMKRHCTISTYLDGQGIGSAK
WDFY4_otoGar1 -IMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGSAK
WDFY4_tupBel1 -VMEPEDDAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEVKRSCTVSTYLDGQGIGSAK
WDFY4_mm9_18_ DAMEPEDEAEPSAGRQLQVRCSQ L LTCGQWYHLAVVVSKEMKRNCSVTTYLDGQAIGSAK
WDFY4_rn4_18_ DIMEPEDEAEPSAGRQLQVRCSQ L LACGQWYHLAVVVSKEMKRNCTVTMYLDGQAIGSAK
WDFY4_dipOrd1 DIMEPEDEGEPSAGRQLQVRCGQ H LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGLAK
WDFY4_cavPor3 DFMEPEDTIEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGSAK
WDFY4_speTri1 DIMEPEDESEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCIISTYLDGQVIGSAK
WDFY4_oryCun1 DVMEPEDDAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQLTGSAK
WDFY4_ochPri2 DVMEPEDDAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQLTGSAK
WDFY4_turTru1 DVMEPEGDPEPSAGRQLRVRCGQ M LACAQWHHLAVVVTKEMKRNCTVSTYLDGQVVGSAK
WDFY4_bosTau4 DVMELEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVFTYLDGQVIGSAK
WDFY4_equCab2 DIMEPEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAVGSAK
WDFY4_felCat3 DIMEPEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQIVGSAK
WDFY4_canFam2 DVMEPEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQITGSAK
WDFY4_myoLuc1 DVMEPEDNAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGSAK
WDFY4_pteVam1 DVMEPEDDSEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKKNCTVSTYLDGQVIGSAK
WDFY4_sorAra1 DVMEPEEDFEPSAGRQLRVRCGQ L LTCGQWHHLTVVVTKEMKRNCTISAYLDGQVIGSAK
WDFY4_loxAfr2 -AMEPEDVAEPSAGRQLQIRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQIIGSAK
WDFY4_proCap1 DTMEPEDVAEPSAGCQLQVRCGQ L LACGQWHHLAVVVNKEMKRNCTVSTYLDGQIIGSAK
WDFY4_echTel1 DAMEPEGDAEPSAGCQLQVKCGQ L LACGQWHHLAVVITKEMKRNCIVSTYLDGQIIGSAK
WDFY4_dasNov2 -AMEPEDAAEPSAGCQLQVRCGQ Q LTCGKWYHLVVVVTKEMKRNCTVSTYLDGQIIGSAK
WDFY4_choHof1 DVMEPEDDTEPSAGRQLQVRCGQ L LACGQWYHLVVVVTKEMKRNC-ISTYLDGQLIGSAK
WDFY4_monDom4 DVMEPEDIHEPSAGSRLQFHCGN L LSSGQWHHLAVVVSKEMKRNCAVSTYINGQLIGSAK
WDFY4_ornAna1 DIMEPEETSEPPAGSRVQFKCVK L ITTGQWHHLAIVVAKEMKRTCVVRAFIDGQLVGSAK
WDFY4_galGal3 DIMEPEGEVQPFPE-QVQFGCGK L LVTGQWHHLTVTVAKEAKKNCTVSAFINGQMLGSAK
WDFY4_taeGut1 DIMEPEGEVLPFPG-QVKFGCGK L LVTGQWHHLTVTVAKEAKKSCIVAAYINGQMLGSAK
WDFY4_tetNig1 DIMEAEVYSDITA-R-LRFRCSS M LIPGQWHHLVVVMTKDVKKSCVTSVYFNGKAFGSGK
WDFY4_fr2_18_ NIMEPEVHSYITP-R-LRFRCSN M LVPGQWHHLAVVMSKDVKKSCVTSVYFNGKAFGSRK
WDFY4_gasAcu1 DMMEPEVLPHPFD-R-LRFQCSS M LVPGQWHHLAVVLSKDVKKSCIASAYFNGKAVGTGK

Case of XYLT1

chr6_2360 XYLT1 5  61 D=3(110) A=5(107)
>contig00001  length=488   numreads=10
RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPI
....L........................................................D.....
                                                             ^

This non-conservative change A-->D is backed by three Sarcophilus reads. However all three are fairly near the end of a minus strand read so none cover the whole exon (raising mild concerns over read quality, given the unusual c-->a base transversion), yet none are long enough to span the next intron to reach the short following exon (leaving some mild pseudogene and paralog issues). Although blastn of extended opossum dna shows that the expected downstream phase 2 splice donor is present, that would also be expected in a close paralog or segmental duplication.

Pseudogene issues: None observed in any mammal using tblastn at wgs database. The detection technique here is a multi-exon query. Because the target database is genomic, recent processed pseudogenes actually give stronger matches because of longer contiguous matches, whereas ortholog matches are weakened by the attempt by blast to extend them. Hence processed pseudogenes surface at the top of match list.

Only a fragment of the gene can be recovered from current Sarcophilus reads, about 8 of 12 exons. However it cannot be determined without genomic assembly which exons 'belong' to the D containing exon, nor can the risk of including matches from the paralog be excluded. This gene has so-so conservation between human and opossum (270 myr roundtrip), 78% identity. which is somewhat puzzling in view of its enzymatic importance. However within marsupials conservation of most exons is in the mid-90's.

Paralog issues: XYLT2 (xylosyltransferase II) gives a moderate match but is not an issue in terms of accurately scoring tasmanian devil populations for the A-->D change. It does create problems in conserved exons in recovering full length genes in species where reads span only single exons. Note XYLT2 also has a conserved A at this position in all 34 available species back to lamprey, proving it an important invariant. Adjacent residues however are only moderately conserved.

XYLT1_homSap RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNAR
             RS+YLHR+V+++++ Y NVRVTPWRM TIWGGASLL+ YL+SMRDLLE+  W WDFFINLSA DYP RTN++LVAFLS+ RD NFLKSHGRDN+R
XYLT2_homSap RSDYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYPTRTNEELVAFLSKNRDKNFLKSHGRDNSR

Homoplasy (recurrent mutation) issues: None.

Known variations: Not a known disease gene; no relevent human variants known.

Side issues: None.

Structural significance: The region enveloping the key residue has a weak match that may nonetheless adequate for structural modeling. The determined structure is a somewhat similar enzyme, 6-N-Acetylglucosaminyltransferase, a product of the GCNT1 gene. A glycine has replaced the alanine, showing the latter is not a deep invariant critical to this class of enzyme. The domain match is pfam02485, defined as 'core-2/I-branching' reflecting the branch the added carbohydrate introduces to the growing chain in chondroitin and heparan sulfate and post-translational protein modifications.

Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 1/59 (1%)

XYLT1_homSap YSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLV  74
             + NV V     + ++   + +   L  M+DL  M +  W + INL   D+PI+TN ++V
GCNT1_musMus FDNVFVASQLESVVYASWTRVKADLNCMKDLYRM-NANWKYLINLCGMDFPIKTNLEIV  193

Functional significance: The protein has been the subject of about a dozen publications. Xylosyltransferases I and II are the chain-initiating enzymes in the biosynthesis of glycosaminoglycans. XYLT1 is the initial and rate-limiting enzyme, transfering UDP-xylose to specific serine residues of a target protein. It is localized to the endoplasmic reticulum and Golgi apparatus as a single-pass membrane protein, but with some fraction also secreted to the extracellular space. The precise function of XYLT2 has not been established.

Note the immediately preceding residues NLS constitute a potential glycosylation site. This is likely realized given the localization of the enzyme. NLS is invariantly conserved in both XYLT1 and XYLT2. While adjacent residues are not normally considered part of the NxT/S motif, potentially the substitution of D could interfer with this post-translational modification. In any event, the glycosylated serine would be at the surface of the protein and so predict the location of the A/D as well.

Natural human polymorphisms in XYLT1 have been observed, P325R, P766A, V8391 and R892Q but these do not include changes at the locus under consideration here. Some 19 residues have been subject to experimental mutation though none of the glycosylation sites.

It is known that formation of abdominal aortic aneurysms is caused by a destructive remodeling of the extracellular matrix in the vascular wall -- A115S enhances this risk. This bears no apparent relation to the A424D allele (human numbering) in tasmanian devil. The 745DWD747 motif has been shown essential to catalytic activity but again lacks immediate relevence. Reduced XYLT1 activity is a known contributor to male sterility.

In summary, this putative change in tasmanian devil needs additional validation. While not likely linked to facial tumors, the A-->D allele is very undesirable in an inbreed population in view of its role in aortic aneurisms and male sterility. The several billion years of branch length invariance of the alanine argues for no tolerance for variation at this position.

        exon 5                                                        ^       exon 6   
hg18_5_ RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
panTro2 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
gorGor1 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSXGRDNA-
ponAbe2 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
rheMac2 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
calJac1 RSNYLHRQVLQFSRQYGNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
tarSyr1 RSNYLHRQVLQFARQYDNIRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNA-
otoGar1 RSNYLHRQVLQFARQYGNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR ---------------------------
tupBel1 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
mm9_5_1 RSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
rn4_5_1 RSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
dipOrd1 RSNYLHRQVLQFATQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLQMPDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
cavPor3 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMQDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
speTri1 RSNYLHRQVLQFAGQYGNVRVTPWRMATIWGGA SLLATYLQSMRDLLEMTDWPWDFFINLSAADYPIR ---------------------------
ochPri2 RSNYLHRQVLQMARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMPDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
vicPac1 rSDYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR ---------------------------
bosTau4 rSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
equCab2 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
felCat3 RSNYLHRQVLQFARQYDNVRVTPWRMATIWGGA SLLSTYLQGMRDLLEMTDWPWDFFINLSAADYPIR ---------------------------
canFam2 RSNYLHRQVLQFARQYGNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
myoLuc1 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLATYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
pteVam1 RSNYLHRQVVQVARQYDNVRVTPWRRATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR ---------------------------
loxAfr2 RSNYLHRQVLZFARQYANVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
proCap1 RSNYLHRQVLQLARQYPNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTSWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
echTel1 RSNYLHRQVLQFTGQYDNVRVTPWRMATIWGGA SLLTTYLQSMRDLLEMADWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
dasNov2 RSNYLHRQVLQFARQYANVRITPWRMATIWGGA SLLSTYLQSMRDLLEMSDWPWDFFINLSAADYPIR ---------------------------
monDom4 RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
macEug  RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
sacHar1 rSNYLHRQVLQFAGQYQNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIr TNDQLVAFLSRYRDMNFLKSHGRDNAR
sacHar2                                   SLLSTYLQSMRDLMEMTDWPWDFFINLSDADYPIr TNDQLVAFLSRYRDMNFLKSHGRDNAR
ornAna1 RSNYLYRQVLQFAGQYPNVRVTSWRMATIWGGA SLLTTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYREMNFLKSHGRDNAR
galGal3 RSNYLHRQVLQFANQYPNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMNDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
taeGut1 RSNYLHRQVLQFASQYPNVRVTSWRMATIWGGA SLLTTYLQTMKDLMEMSDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR
xenTro2 RSHYLHRQVLQFASQYPNVRVTSWRMSTIWGGA SLLSTYLQSMRDLLEMSDWSWDFFINLSAADYPVR ---------------------------
tetNig1 RSNYLHRQVQALAALYPNVRVTPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR ---------------------------
fr2_5_1 RSNYLHRQVQALAALYPNVRVTPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR TNDQLVAFLSKYRNMNFIKSHGRDNAR
gasAcu1 RSNYLHRQVLSLAAQYSNVRATPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR ---------------------------
oryLat2 -SNYLHRQVQIMAMKYPNVRVTPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR ---------------------------
danRer5 RSNYLHRQMVALAHQYPNVRVTSWRMSTIWGGA SLLTMYLQSMKDLLAMRDWSWDFFINLSAADYPIR ---------------------------
squAca1 RSNYLHREAMQLAQRYSNIRITPWRMVTIWGGA SLLKMYLHCMKDLLEMTDWQWDYFINLSATDYPTR TNDELMGFLSKYRGKNFLKSHGRDNAR
leuEri1 RSNYLHREVMQLAQQYPNVRVTPWRMVTIWGGA SLLKMYLNCMKDLLEMTDWHWDYFINLSATDYPTR TNDELVGFLSRYREKNFLKISR-----
petMar1 RSNYLQRQVLQVAERYPNVRVTPWRMATIWGGA SLLTMYLRTMKDLLDMADWAWDFFINLSATDYPIR TNDQLVAFLTKYRDKNFLKSHGRDNNR

The A is also conserved in the paralog XYLT2:
                                                                           ^
XYLT2_hg18_4_ RSDYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_gorGor1 RSNYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_ponAbe2 RSNYLHREVVELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_rheMac2 RSDYLHREVVELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_calJac1 RSNYLHREVAELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_tupBel1 RSNYLHREVVELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_mm9_4_1 RSNYLYREVVELAQHYENVRVTPWRMVTIWGGASLLRMYLRSMKDLLEIPGWTWDFFINLSATDYPTR
XYLT2_rn4_4_1 RSNYLYREVVELAQHYDNVRVTPWRMVTIWGGASLLRMYLRSMKDLLEIPGWTWDFFINLSATDYPTR
XYLT2_dipOrd1 RSDYLHREVVELAKQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR
XYLT2_cavPor3 RSNYLHREVVALAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_speTri1 RSNYLHREVVELAQRYENVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_ochPri2 ---YLHREVVELAQQYENVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWTWDFFINLSATDYPTR
XYLT2_turTru1 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPDWAWDFFINLSATDYPTR
XYLT2_bosTau4 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR
XYLT2_equCab2 RSNYLHREVVELARQYDNVQVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR
XYLT2_felCat3 RSNYLHREVVELARRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_canFam2 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR
XYLT2_myoLuc1 RSNYLHREVVELARQYDNIRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR
XYLT2_pteVam1 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_eriEur1 RSNYLHREVVELARHYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR
XYLT2_proCap1 RSNYLHREVVELARQYDNMRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR
XYLT2_monDom4 RSNYLHREVVALAQHYANVRVTPWRMGTIWGGASLLKMYLRSMQDLLEAPGWTWDFFINLSATDYPTR
XYLT2_macEug  RSNYLHREVVALAQHYANVRVTPWRMGTIWGGASLLKMYLRSMQDLLEAPGWTWDFFINLSATDYPTR
XYLT2_sarHar  RSNYLHREVVALAQHYANVRVTPWRMGTIWGGASLLKMYLRSMQDLLEAPGWTWDFFINLSATDYPTR
XYLT2_galGal3 RSNYLHREAVELAQHYPNIRVTPWRMVTIWGGASLLKMYLRSMKDLLELTEWPWDFFINLSATDYPTR
XYLT2_taeGut1 RSSYLHREAVELARHYPNIRVTPWRMVTIWGGASLLKMYLRSMKDLLELSEWPWDFFINLSATDYPTR
XYLT2_anoCar1 RSTYLHREVVEMAQHYPNIRVTPWRMVTIWGGASLLKMYLHSMKDLLEMTDWTWDYYINLSATDYPTR
XYLT2_xenTro2 RSNYLHREVVRLAQSYENMRVTPWRMVTIWGGASLLTMYLRSMKDLLEVPDWPWDFFINLSATDYPTR
XYLT2_tetNig1 RSGYMHREVLQVAQQYPNIRATPWRMVTIWGGASLLKAYLHSMQDLLSMLDWKWDFFINLSATDFPTR
XYLT2_fr2_4_1 RSNYLHRQVQALAALYPNVRVTPWRMATIWGGASLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR
XYLT2_gasAcu1 RSNYLHRQVLSLAAQYSNVRATPWRMATIWGGASLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR
XYLT2_oryLat2 RCSYMHREVLQMAKHYPNIRATPWRMVTIWGGASLLKAYLRSMQDLLSMAEWKWDFFINLSATDFPTR
XYLT2_danRer5 RSNYLHRQMVALAHQYPNVRVTSWRMSTIWGGASLLTMYLQSMKDLLAMRDWSWDFFINLSAADYPIR
XYLT2_petMar1 RSNYLQRQVLQVAERYPNVRVTPWRMATIWGGASLLTMYLRTMKDLLDMADWAWDFFINLSATDYPIR

>XYLT1_homSap
MVAAPCARRLARRSHSALLAALTVLLLQTLVVWNFSSLDSGAGERRGGAAVGGGEQPPPAPAPRRERRDLPAEPAAARGGGGGGGGGGGGRGPQARARGGGPGEPRGQQPASRGALPARAL
DPHPSPLITLETQ
DGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPPSRQKELLKRKLEQQEKGKGHTFPGKGPGEVLPPGDRAAANSSHGKDVSRPPHARKTGGSSPETKYDQPPKCDISGKEAISALSRAKSKHCRQEIGETYCRHKLGLLMPEKVTRFCPLE
GKANKNVQWDEDSVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDK
RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR
TNDQLVAFLSRYRDMNFLKSHGRDNAR
FIRKQGLDRLFLECDAHMWRLGDRRIPEGIAVDGGSDWFLLNRRFVEYVTFSTDDLVTKMKQFYSYTLLPAE
SFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQ
QTARPTFFARKFEAVVNQEIIGQLDYYLYGNYPAGTPGLRSYWENVYDEPDGIHSLSDVTLTLYHSFARLGLRRAETSLHTDGENSCR
YYPMGHPASVHLYFLADRFQGFLIKHHATNLAVSKLETLETWVMPKKVFKIASPPSDFGRLQFSE
VGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSNRQPIKP
EEALKLHNGPLRNAYMEQSFQSLNPVLSLPINPAQVEQARRNAASTGTALEGWLDSLVGGMWTAMDICATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR*

>XYLT1_monDom
MVAALCARRLARRSHSALIAALTVLLLQTLIVWNFSSLDSGAGDHRGGAAAGGPPPAPRRERRDLPLEPAAAGEGERGPAGGQLLRERGGGHGEHRAQHPPRRGGLPGRAL
EPPPSPFTSLETQ DGYFSHRPKEKMRTDSNNENSVPKDFENIDNSNFAPRTQRQKHQPDLGKKPLSKQKEHLKKKLEQDEKVKENSLLGKGSNEALQYSNQAAQNSSQGKKSSRLPHSRKNGAGSPELKYDQPPRCDISGKEAISALSRSKSKQCRQEIAEIYCQHKVGKLMPEKVTRFCPLE
GKANNNVRWDEDSVEYMPANPVRIVFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDK
RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR
TNDQLVAFLSRYRDMNFLKSHGRDNAR
FIRKQGLDRLFLECDTHMWRLGDRKIPEGITVDGGSDWFLLNRKFVEYVTFSNDDLVTKMKQFYSYTLLPAE
SFFHTVLENSPHCGTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPADFHRFQ
QTARPTFFARKFEAVVNQEIIGQLDYYLYGNYPSGTPGLRSYWENVYDEPDGIHSISDVVLTMYHSFTRLGLRRAETSLHTDGENSCR
YYPMGHPVSVHLYFLADHFQGFLIKHHATNLAVSKLETLETWVMPKKVFKIANPPSDFGRLQFSE
IGTEWDAKERIFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNIIAATYDILIESSAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVTPLTFSNKQPIKP
DESLKLHNGPPRNAYMEQSFQGLNPVLNIPINLAHVEQARRNAATTGAKLESWVDSLVGGIWSAVDICAIGPTACPVMQTCSQTSWSSLSPDPKSELGAIKPDGRLR*

>XYLT1_macEug fragment
MVAALCARRLARRSHSALIAALTVLLLQTLIVWNFSSLDSGAGDHRGGEQHAGGEPPPAPRRERRDLAPESRAAAGEEGGGGGRGPQPRGYKLPLERGGGGGGGHREHRPQQTPRRGGPAAGAAQLPGQAL
...
DGYFSHRPKEKMRTDSNNENSVPKDFENIDNSNFAPRTQRQKHQPDLGKKSLSKQKEQLKKKLEQEEKAKENSLLGKSSNEAMQYSNQAAQNSSAAKASPKSSKQPHTRKNGAGSPELKYDQLPRCDISGKEAISALSRSKSKQCRQEIAEIYCQHKVGKLMPEKVTRFCSLE
GKANNNVRWDEDSVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDK
RSNYLHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR
...
FIRKQGLDRLFLECDTHMWRLGDRKIPEGITVDGGSDWFLLNRKFVEYVTFSNDDLVTKMK...
SFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPADFHRFQ
...
YYPMGHPVSVHLYFLADRFQGFLIKHHATNLAVSKLETLETWVMPKKVFKIANPPSDFGRLQFSE
IGTDWDAKERIFRNFGGLLGPKDEPVGMQKWGKGP...
DESLKLHGGPPHNAYMEQSFQGLNPVLNIPINLAHVEQARRNAATTGPKLESWVDSLVGGVWSAMDICAIGPTACPVMQTCSQTSWSSLSPDPKSELGAVKPDGRLR*

>XYLT1_sarHar fragment missing 5-6 exons
...
...
DGYFSHRPKEKMRTDSNNENSVPKDFENIDNSNFAPRTQRQKHQPDLG...PHVRKNGVGSPELKYDQPPRCDISGKEAISALSRSKSKQCRQEIAEIYCQHKVGKLMPEKVTRFCPL.
rSNYLHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR
TNDQLVAFLSRYRDMNFLKSHGRDNAR
FIKKQGLDRLFHECDSHMWRLGERQIPEGIVVDGGSDWFALTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAE
SFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPADFHRFQ
...
YYPMGHPVSVHLYFLADRFQGFLIKHHATNLAVS...
IGTDWDAKERIFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESSAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVTPLTFSNRQPIKP
DESLKLHNGPPRNAYMEQSFQGLNPVLNIPINLAHVEQARRNAAITGPKLENWVDSLVGGIWSAVDICAIGPTACPVMQTCSQTSWSSLSPDPKSELGAIKPDGRLR*




<pre>

=== Case of ATP4A === 

 chr4_18550 ATP4A 6 16 C=4(130) R=3(74)
 >contig00001  length=906   numreads=10
 TAQGLVVNTGDRTIIGRIASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFIVAMCIGYTFLRAMVFFMAIVVAYVPEGLLATVT
 ................C........................................................................
                 ^

=== Case of VPS72 ===

 chr2_30280 VPS72 5  15 R=3(59) K=2(51)    
 >contig00001  length=591   numreads=6
 NYERLEADKKKQVHKKRKCPGPVITYHSMTVPLLAEPGPKEENVDVE
 ...............R..................T............
                ^
 
=== Case of ABCC1 ===

 chr6_5144 ABCC1 23  4 Q=2(69) P=2(80) looks like a frame-shift problem in monDom5
 >contig00001  length=802   numreads=10
 HLCFPRLHLDLLHNVLRSPMSFFERTPSGNLVNRFSKEMDTVDSMIPQIIKMFMGSLFNVIGACIIILLATPIAAIIIPPLGLIYFFVQ
 ....Q....................................................................................
     ^

== Discarded candidates ==

Below are three initial candidates that had to be discarded without detailed followup. One arose from repeated frameshifts in the critical region, another exhibited homoplasy with marsupials, and the third too extensive of an accepted reduced alphabet at the site. Thus while these three genes do not meeet the search criteria, they are nonetheless instructive in illustrating those criteria and making clear these are quite restrictive.

=== Case of ACOT12 ===

 chr3_5872 ACOT12 14 14 I=3(95) V=3(110) 'wobbly'
 >contig00001  length=472   numreads=6
 NTYVVAVKSVTLASIPPSPQYNRSEITCAGFLIRAVDSNSCT
 .................................Q....S...
               ^
Here an I-->V change is seen in some tasmanian devils reads relative to opossum and wallaby. Here V is more typical of a theran mammal. Note I is also seen in armadillo, a placental, and A occurs in platypus and various other mammals. ACOT12, a acyl-CoA thioesterase, does not track back well in earlier diverging species. Because of the observed homoplasy, this locus is an unsuitable example of a significant amino acid change in Sarcophilus. However it illuminates the nature of suitable candidates and so is retained here.
                               ^
 ACOT12_hg18_14 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI
 ACOT12_panTro2 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI
 ACOT12_gorGor1 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI
 ACOT12_ponAbe2 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI
 ACOT12_rheMac2 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSSSCI
 ACOT12_calJac1 NTYTVAVKSVMLPS V PPSPQYIRSEIICAGFLIHAIDSNSCI
 ACOT12_micMur1 NTYTVAVKSVILPS V PPSPQHVRSEIICAGFLIHAADSNSCT
 ACOT12_otoGar1 NTYMVAAKSVILPS V PPSPQYIRSEIICAGFLIHTIDSTSCT
 ACOT12_tupBel1 NTYTVAVKSVTLPS V PPSPQYIRSDIICAGFLIRPVDSSSCT
 ACOT12_mm9_14_ NTYTVALRSVVLPS V PSSPQYIRSEVICAGFLIQAVDSNSCT
 ACOT12_rn4_14_ NTYIVALMSVVLPS V PPSPQYIRSQVICAGFLIQPVDSSSCT
 ACOT12_dipOrd1 NTYVVATKSVILPS V PPSPAYIRSEAVCSGFLIKAVDSSSCT
 ACOT12_cavPor3 DTYLVAVKSVVLPA V PPSPGYTRSEVALAGFLIQPTDHSSCT
 ACOT12_oryCun1 HAYTVAAKSVMLPS A PPSPDHTRSEIICAGFLIHAIDSHSCT
 ACOT12_ochPri2 HAYVVAVKSVVLPS A PPSPEYIRGEIVCAGFLIHAIDSHACT
 ACOT12_vicPac1 NTYTVAVKSVILPS V PPSPQYVRSEITCAGFLIHAIDNSSCT
 ACOT12_turTru1 HTYTVAVRSVILAS V PPSPQYSRSEIISAGFLIRAIDSSSCT
 ACOT12_bosTau4 HTYVVAVRSVILPS V PPSPQYVRSEIECAGFLIHATDSSSCT
 ACOT12_equCab2 KTFSVAAKSVILPS V PPSPQYMRSEIRCAGFLICAIDNSSCT
 ACOT12_felCat3 STYTVAVKSVLLPS V PPCPHYIRSEIICAGFLIRAIDSSSCT
 ACOT12_canFam2 NTYTVAVKSVTLPS V PPSPQYSRSEILCAGFLIHAIDSSSCT
 ACOT12_myoLuc1 NTYTVAVKSVILPS V PPSPQYVRSEIICAGFLIHAIDSSSCT
 ACOT12_pteVam1 NTYTVAVKSVILPS V PPSPZYVRSEIVCAGFLIHAIDGSLCI
 ACOT12_eriEur1 STFTVAMKSVLLAS V PSSPQYIRSEITCAGFVIHAVSSNSCI
 ACOT12_sorAra1 NAFTVAVKSVILPS V PPSPQYMRSEIICAGFLIHATDSNSCI
 ACOT12_loxAfr2 D--TVAVKSVLLPS V PPCPQYIRSEIIRAGFLIHTIDSNSCT
 ACOT12_echTel1 TTYTVALRSVLLPS V PSSPNYVRGEIICAGFLVHPIDSSACT
 ACOT12_dasNov2 NTYTVAVKSVVLPS I PPSPQYIRSEIICAGFLIHAIDSSSCT
 ACOT12_choHof1 NSYTVAAKSVVLPS V PPSPQYIRSETICAGFLINAIDSSSCT
 ACOT12_monDom4 NTYVVAVKSVTLAS I PPSPQYNRSEITCAGFLIRAVDSNSCT
 ACOT12_macEug  NTYVVAMKSVTLAS I PPSPQYNRSEITSAGFLIQAVDSNSCT
 ACOT12_sacHar1 NTYVVAVKSVTLAS I PPSPQYNRSEITCAGFLIQAVDSSSCT
 ACOT12_sacHar2 NTYVVAVKSVTLAS V PPSPQYNRSEITCAGFLIQAVDSSSCT
 ACOT12_ornAna1 DSYLVAVKSVILAS A PPSHQYIRSEIPCAGFLVEALDSSSCK

=== Case of FLI1 ===

 chr4_11174 FLI1 3  32 N=2(63) K=3(47)
 >contig00001  length=575   numreads=9
 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEKNGPPPNMTTNERRVIVPA
 ..................................................
                                 ^
Here the N-->K change is a non-conservative substitution in the sense asperagine is merely polar whereas lysine is bulkier and negatively charged. The N is highly invariant at this position back to teleost fish. FLI1 is a transcription factor associated with a leukemia virus integration site and Ewing sarcoma.

This would be a promising candidate except for the fact that the three reads establishing K clearly are plagued by frameshifts at the critical region. Possibly anomalous base composition is responsible here (ggatgagaagaacggcccccctcc) -- which is no doubt giving rising to transcriptional slippage generating homoplasic deletions of polyP -- or perhaps low coverage. This change is unlikely to be validated upon additional bulk or targeted sequencing because these lack motivating evidence.

 >FP1JAYN01BA7O5 and FP5M7SR01ERAQP  Frame = +1        Frame = +2
 
 Query: 1  ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEKNGPP 36   KNGPPPNMTTNERRVIVPA 50
           ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK   P      KNGPPPNMTTNERRVIVPA
 Sbjct: 37 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEKKRSP 144  KNGPPPNMTTNERRVIVPA 187
 
 >FP1JAYN01DX0A1 length=254
 
 Query: 1  ESPVDCSVNKCSKLVGGNESNPMN-YNTYMDEKNGPPPNMTTNERRVIVPA 50
           ESPVDCSVNKCSKLVGGNESNPMN  + +  EK  PPPNMTTNERRVIVPA
 Sbjct: 37 ESPVDCSVNKCSKLVGGNESNPMNLQHLHG*EKTVPPPNMTTNERRVIVPA 189
 
   N  P  M  N  Y  N  T  Y  M  D  E  K  N  G  P  P  P  N  M  T  
   |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  
  aatcctatgaattacaatacctacatggatgagaagaacggcccccctcctaacatgacc 
 
 FLI1_hg18_3_ ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_panTro2 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_gorGor1 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_ponAbe2 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_rheMac2 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_calJac1 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_tarSyr1 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_micMur1 ESPVDCSVSKCGKLIGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_otoGar1 ESPVDCSVSKCSKLIGGGEANPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_mm9_3_9 ESPVDCSVSKCNKLVGGGEANPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_rn4_3_9 ESPVDCSVSKCNKLVGGGEANPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_dipOrd1 ESPVDCSVSKCSKLVGGGESNPMNYNSYIDEK N GPPPPNMTTNERRVIVPA
 FLI1_cavPor3 ESPVDCSVSKCSKLVGTGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_speTri1 ---VDCSVSKCSKLVFGGESNPMNYNSYLDEK N GPPPPNMTTNERRVIVPA
 FLI1_oryCun1 ESPVDCSISKCGKLVGGGEANAMSYNNYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_vicPac1 ESPVDCSVSKCGKLVGGGESNTMSYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_turTru1 ESPVDCSVSKCGKLVGGGESNAMSYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_bosTau4 ESPVDCSVSKCGKLVGGGESNTMSYTSYVDEK N GPPPPNMTTNERRVIVPA
 FLI1_equCab2 ESPVDCSVSKCSKLVGGGESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_canFam2 ESPVDCSVSKCSKLVGGSESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_myoLuc1 ESPVDCSVSKCSKLVGGGESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_pteVam1 ESPVDCSVSKCSKLVGGGESNAMNYNSYIDEK N GPPPPNMTTNERRVIVPA
 FLI1_eriEur1 ESPVDCSVSKCSKLVGGGESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_proCap1 ESPVDCSVSKCSKLAGGGESNPMNYNTYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_dasNov2 ESPVDCSVSKYSKLVGGGESNPMTYSTYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_choHof1 ESPVDCSVSKCSKLVGGGEATPMTYNTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_monDom4 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_macEug  ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_sarHar1 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_ornAna1 ESPVDCSVSKCGKLVGSGESNPMNYNSYMEEK N GPPPPNMTTNERRVIVPA
 FLI1_galGal3 ESPVDCSVNKCSKLVGAGESNPMSYSTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_taeGut1 ESPVDCSMNKCGKLVGAGESNPMSYSTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_anoCar1 ESPVDCSVSKCNKLVPAGESNSLNYGTYMDEK N GPP-PNMTTNERRVIVPA
 FLI1_xenTro2 ESPVDCSISKCSKLIGGSENNAVTYNSYMDEK N GPPPPNMTTNERRVIVPA
 FLI1_tetNig1 ESPVDCSVGKCNKLVGGNDVSQMSYGSYMDEK N APP-PNMTTNERRVIVPA
 FLI1_fr2_3_9 ESPVDCSVGKCNKLVGGNDVSQMNYGSYMDEK N APP-PNMTTNERRVIVPA
 FLI1_gasAcu1 ESPVDCSVGKCNKLVGSNDTSQMNYGNYMDEK N APP-PNMTTNERRVIVPA
 FLI1_oryLat2 ESPVDCSVGKCNKLVGGNDTSQMTYGNYMDEK S APP-PNMTTNERRVIVPA
 FLI1_danRer5 ESPVDCSVGKCNKMVGGTEASQMNYTGYMDEK C APP-PNMTTNERRVIVPA

=== Csae of SPON1 ===
 
 chr5_8347 SPON1 11  20 V=3(65) I=2(66) wobbly
 >contig00001  length=433   numreads=5
 GSTCTMSEWITWSPCSISCGVGMRSRERYVKQFPEDGSVCTVPTEETEKCTVNEEC
 ......................................I.N...............
                     ^
Here two Sarcophilus reads show V-->I following residue 20 while three are V like opossum. It quickly emerges that wallaby also has I. Thus the change in tasmanian devil is within the normal reduced alphabet of this residue position. Various placentals show that T and M and even P are also accepted substituents here. Note too these are used clade-incoherently (eg primates alone are variable). Consequently this site is not under strong selection for V to begin with so SPON1 does not meet the selection criteria being used here.

                                     ^
 SPON1_hg18_13 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_panTro2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_gorGor1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_ponAbe2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_rheMac2 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_calJac1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_tarSyr1 -GSTCTMSEWITWSPCCLSCV P GMRSREYYLK-FFEDGSVCSLTPKKTQNRTV-EZC
 SPON1_micMur1 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_otoGar1 DGSTCTMSEWITW-PCSISCG T GMRSRERYVKQFPEDVSVCTLPTEETEKCTVNEEC
 SPON1_tupBel1 EGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_mm9_13_ DGSTCTMSEWITWSPCSVSCG M GMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEEC
 SPON1_rn4_13_ DGSTCTMSEWITWSPCSVSCG M GMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEEC
 SPON1_dipOrd1 -GSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_cavPor3 DGSTCTMSEWIIWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_speTri1 EHSTCTMSEWITWSPCCISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_oryCun1 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_ochPri2 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_turTru1 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPT-ETEKCTVNEEC
 SPON1_bosTau4 DGSTCTMSEWITWSPCSISCG T GTRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_equCab2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_canFam2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_myoLuc1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_pteVam1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_eriEur1 DGSACTMSEWITWSPCSLSCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_sorAra1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_proCap1 -GSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_echTel1 ----CPMSEWITWSPRSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_dasNov2 -GSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC
 SPON1_monDom4 DGSTCTMSEWITWSPCSISCG V GMRSRERYVKQFPEDGSVCTVPTEETEKCTVNEEC
 SPON1_macEug   GSTCTMSEWMTWSPCSISCG I GMRSRERYVKQFPEDGSVCTVPTEETEK
 SPON1_sacHar1  GSTCTMSEWITWSPCSISCG V GMRSRERYVKQFPEDGSICNVPTEETEKCTVNEEC 
 SPON1_sacHar2  GSTCTMSEWITWSPCSISCG I GMRSRERYVKQFPEDGSICNVPTEETEKCTVNEEC
 SPON1_ornAna1 DGSTCTMSEWITWSPCSVSCG M GMRSRERYVKQFPDDGSMCKVPTEETEKCVVNEDC
 SPON1_anoCar1 DGSTCMMSEWITWSPCSVSCG M GMRSRERYVKQFPDDGSMCKVPTEETEKCIVNEEC
 SPON1_xenTro2 EASTCMMSEWITWSPCSASCG M GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEEC
 SPON1_tetNig1 DASTCMMSEWITWSPCSASCG M GSRSRERYVKQFPDDGSICTLPTEETEDCVVNEEC
 SPON1_fr2_13_ DASTCMMSEWITWSPCSASCG M GSRSRERYVKQFPDDGSICTLPTEETEDCVVNEEC
 SPON1_gasAcu1 DASTCMLSEWITWSPCSLSCG M GTRSRERYVKQFPDDGSLCSLPTEETDNCVVNEEC
 SPON1_oryLat2 DGSTCMMSEWITWSPCSMSCG A GIRSRERYVKQFPDDGSICTLPTEETENCVVNEEC
 SPON1_danRer5 DSSTCMMSEWITWSPCSVSCG S GLRSRERYVKQFPDDGFACTHPTEETEPCTVNEEC

== Other marsupial genes of interest ==

The collections below contain well-understood genes with very extensive comparative genomics. They can serve as a test bed for  Sarcophilus assembly quality, a place where genuine anomalies or distinct adaptive features might surface (perhaps as phyloSNPs) and where marsupial phylogeny might be refined using rare genomic events in nuclear genes. The gene sets contain all available marsupial orthologs plus for context one flanking gene each from placentals and monotremes. These genes are available in much broader hand-curated sets elsewhere on this site.

Scattered new data is available for other marsupials and monotremes from 454 reads, Sanger trace data and transcripts:

 Didelphis virginiana       88,207 traces 248 nuc
 Trichosurus vulpecula     169,115 traces 321 nuc  147,199 ests
 Sminthopsis crassicaudata                 59 nuc    1,669 ests
 Sminthopsis macroura        3,411 traces  89 nuc
 Isoodon macrourus           6,144 traces, 70 nuc    1,319 ests
 Tachyglossus aculeatus     93,653 traces 243 nuc
  
 SRX000015  Baylor  454 sequencing of Monodelphis domestica genomic fragment library
 SRX000086  WUGSC   454 sequencing of Macropus eugenii genomic fragment library
 SRX000186  WUGSC   454 sequencing of Ornithorhynchus anatinus transcript 
 SRX000122  WUGSC   454 sequencing of Tachyglossus aculeatus transcript
 SRX000121  WUGSC   454 sequencing of Tachyglossus aculeatus transcript

The running estimate of coverage of Sarcophilus genome combining all runs for 11 expected genes on different chromosomes:
 
 59 of 68 exons found (87%)
 3883 of 4339 amino acids available (89%)

Newbler has a bad tendency to create non-existent frameshifts as seen in these three reads for the same query gene:

 Query: 82  ggtctctacggcagtgtcattgtcactggagggaacacactcttgcaagg
            |||||||||||||||||||||| |||||||||||||||||| ||||||||
 Sbjct: 167 ggtctctacggcagtgtcattg-cactggagggaacacactgttgcaagg  FP1I63R01APY7E 
 
 Query: 82  ggtctctacggcagtgt-cattgtcactggagggaacacactcttgcaagg
            ||||||||||||||||| |||||||||||||||||||||||| ||||||||
 Sbjct: 268 ggtctctacggcagtgttcattgtcactggagggaacacactgttgcaagg FKUJDAX01AWWZ3
 
 Query: 82  ggtctctacggcagtgtcattgtcactggagggaac-acactcttgcaagg
            |||||||||||||||||||||||||||||||||||| ||||| ||||||||
 Sbjct: 268 ggtctctacggcagtgtcattgtcactggagggaacgacactgttgcaagg FKUJDAX01DZSZO

=== IRBP (96 marsupials) ===

Interphotoreceptor retinol-binding protein, poorly named by IGNC as RBP3 despite its complete lack of paralogs, is a 4 exon 1247 residue glycoprotein that shuttles retinoids between the photoreceptor cells and the retinal pigment epithelium. The protein's size results from four ancient internal tandem duplictions that became established prior to the intronation era. 

The first three homology domains and part of the fourth are all encoded by the first large exon of 1090 amino acids. This exon has been much used in marsupial phylogeny (along with the first intron of transthyretin). Indeed the 96 marsupial species in 51 genera having determined IRBP sequences at GenBank include a Dec 2008 partial sequence for [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=158668312 Thylacinus cynocephalus], as well as for [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=47117971 Sarcophilus harrisii].

The closest matches to the thylacine IRBP are shown in the difference alignment of the first 60 residues below. These species all lie with the Dasyuromorphia. The indicated E-->K may be one of several phyloSNPs breaking this group into <font color="blue">blue</font> and <font color="green">green</font> subclades. 

The numbat <font color="red">Myrmecobius</font> fits implausibly (its amino terminal sequence [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=124054062 EF028750] needs verification) -- its affinities seem to lie with the <font color="brown">Didelphimorphia</font>. Thylacinus is not basal within Dasyuromorphia relative to Myrmecobius using IRBP. However this may be a case of mis-comparison of genes.
   *           *                                     *
 <font color="blue">STSKAPQHDSKFTNATQEELLALFQQIIKYQVLEGNVGYLRVDYIPGREMIEEVGEFLVN EU091365  0 Thylacinus cynocephalus
 .........P..A..................I............................ AY532676  3 Myoictis wallacei
 ........NP..A............................................... AY532687  3 Neophascogale lorentzii
 ........NP..A........T...................................... AY532686  4 Phascolosorex dorsalis
 .........P..V............................................... AY532670  2 Parantechinus apicalis
 ....V....P..A..................I.....................L...... AY532675  5 Myoictis melas
 .........P..A...................................D........... AY532679  3 Dasyurus hallucatus
 ...E.....P..A............K........D.............D........... AY532685  6 <font color="magenta">Sarcophilus harrisii</font>
 ...E.......RA..........L............................Q..K.... EF028748  6 Sminthopsis crassicaudata
 .......R.P.LA.........SL.......................Q....Q....... EF028749  8 Planigale ingrami</font>
 <font color="green">..A......P.LA.V.....................................K....... EF028736  6 Antechinus stuartii
 ..A......P.L..V.....................................K....... EF028743  5 Micromurexia habbema
 ..A......P.LA.V.....................................K....... EF028744  6 Murexchinus melanurus
 ..A......P.L..V....V................................K....... EF028746  6 Paramurexia rothschildi
 ..A......P.LA.V.....................................K....... EF028747  6 Phascogale calura
 ..A......P.LA.V.....................................K....... EF028745  6 Phascomurexia naso
 .SA......P.LA.V.....................................K....... AY532667  7 Murexia longicaudata</font>
 <font color="red">......K..PNLA........T.L..R....................Q.VV.K....... EF028750 12 Myrmecobius fasciatus</font>
 <font color="brown">..PET...VP..A.V........L..M....................Q.VV.K....... AY233765 13 Caluromys philander
 ..PET...VP.LA.V.......QL..M....................Q.VV.K....... AF257675 15 Caluromysiops irrupta
 ..PET...VP.LA.V......T.L..M....................Q.VV.K....... AF257688 15 Glironia venusta  
 .IPET...VP..A.V.R....T.L..M....................Q.VV.K....... AF257683 16 Didelphis albiventris
 .IPE....VP.LA.I......T.L..M....................Q.VV.K....... AF257686 15 Gracilinanus microtarsus
 .IPET...VP..A.V......T.L..M....................Q.VV.K....... AF257676 15 Marmosops noctivagus
 .IPET...VP.LA.V........L..M....................Q.VV.K....... AY233788 15 Philander opossum
 .IPET...VP.LA.I......T.L..M....................Q.VV.K....... AF257689 16 Thylamys pallidior</font>

Using Sarcophilus as probe in a different region, 721-900, we find this peculiar outcome: what appears to be a second very odd gene, XY difference, pseudogene, weird balanced polymorphism, nonhomologous recombination, sequence submission error, frameshifts, or systemic experimental error (eg Dasyurus maculatus AY532680 is identical to AY243439 outside the 15 amino acid block). However the genomic reads from individual Sarcophilus used in this project show no sign of this gene despite excellent coverage of the second type of gene.

Macropus and Monodelphis genomes only contain the second type of gene. All Didelphimorphia and Diprotodontia are of this type, as are platypus and all placentals. With the Sarcophilus genome, this can be resolved as it should have both and be the such first genome. Perhaps the alignment above is a mixture of type 1 and type 2 genes (resp. alleles). The Myrmecobius anomaly makes it more likely two distinct genes are present.

A definite pecularity seen in blast searches is the occurence earlier in the sequence of a very homologous segment for this very block, likely the homologous part of another of the internal tandem repeats. It is seen in both types of genes. Possibly internal non-homologus recombination or gene conversion has inserted first repeat sequence again in this distal block in place of what was relatively diverged sequence. Internal gene conversion would make IRBP extremely difficult to use in alignment-based phylogeny. As rare genomic event, it unites the species that have it but species that don't have it would have to be re-examined to exclude the possiblity that only the type 2 gene happened to be sequenced.

It emerges from direct tblastn that the Sacrophilus individual sequenced was female. That is, ATRX is well represented but not ATRY (though the situation is somewhat confused due to additional paralogs). Marsupial XY are [http://www.ncbi.nlm.nih.gov/pubmed/11173870,12508115,16209912,17333539,18185981,9215558 quite different] from placentals: 
<blockquote>
"Many or most genes on the mammal Y chromosome evolved a testis-specific function after diverging from an X-borne copy with a general function in both sexes. In marsupial but not eutherian mammals, a testis-specific orthologue (ATRY) of the widely expressed X-borne ATRX gene lies on the Y chromosome. Since mutations in human ATRX cause sex reversal, it is possible that one function of ATRY in marsupials is testicular differentiation. We report here the isolation and sequencing of the tammar wallaby (Macropus eugenii) ATRY cDNA, and comparison of its sequence with that of tammar ATRX. The evolution of a testis-specific function for the ATRY protein distinct from the general role of ATRX in both sexes has been accompanied by sequence changes in many protein domains that would alter protein binding partners. A large open reading frame encodes a 1771 amino acid ATRY protein that has diverged extensively from ATRX. The conservation and loss of particular motifs identify those required for testicular function (ATRY) and function in other tissues (ATRX)."</blockquote>
 
 AY532685 MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE <font color="blue">Sarcophilus harrisii</font>
 AY532684 ....E................................S....................P. Dasyurus geoffroii
 AY532681 ....E................................S....................P. Dasyurus albopunctatus
 AY532683 ....E................................S....................P. Dasyurus viverrinus
 AY532682 ....E........................P.......SE...................P. Dasyurus spartacus
 AY532680 ....E..............R.................SR...................P. <font color="red">Dasyurus maculatus</font>
 AY532678 ..V..................................S....................P. Dasycercus cristicauda
 AY532669 ..V..................................S....................P. Dasykaluta rosamondae
 AY532676 ..V..................S...............S....................P. Myoictis wallacei
 AY532675 ..V..................S...............S....................P. Myoictis melas
 AY532687 ..V........N.L.......................S....................P. Neophascogale lorentzii
 AY532671 ..V..................................S....................P. Parantechinus bilarni
 AY532670 ..V.................................TS.........RG.........P. Parantechinus apicalis
 AY532686 ..V..................................S........P...........p. Phascolosorex dorsalis
 AY532674 ..V.......................................................P. Pseudantechinus ningbing
 AY532672 ..V..................................S....................P. Pseudantechinus woolleyae
 AY532673 ..V........N..R......................S...................SP. <font color="magenta">Pseudantechinus roryi</font>
 454 read MEILQKYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAMLQAVSEDP           <font color="blue">Sarcophilus harrisii</font>
 EF028739 ............................V.TEEDLAAKLNAMLQA.............P. Antechinus minimus
 AY243439 ....E..............R........V.TEEDLAAKLNAMLQA.............P. <font color="red">Dasyurus maculatus</font>
 EF028750 ....K................KT.....I.TEEDLAAKLNAILQA.............P. Myrmecobius fasciatus
 EF028737 ..V.........................V.TEEDLAAKINAMLQA.............P. Antechinus flavipes
 EF028748 ..V.........................V.TEEDLAAKLNA.LQA.............P. Sminthopsis crassicaudata
 AY243438 ..V.........................V.TEEDLAAKLNA.LQA.............P. Planigale sp.
 EF028749 ..V.........................V.TEEDLAAKLNA.LQA.............P. Planigale ingrami
 AY532679 ..V.........................V.TEEDLAAKLNAMLQA............... Dasyurus hallucatus
 AF025382 ..V.........................V.TEEDLAAKLNAMLQA.............P. Phascogale tapoatafa
 EF028741 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus godmani
 AY532666 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus swainsonii
 EF028736 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus stuartii
 EF028742 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus agilis
 EF028738 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus bellus
 EF028740 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus leo
 EF028747 ..V.........................V.TEEDLAAKLNAMLQA.............P. Phascogale calura
 EF028744 ..V.........................V.TEEDLAAKLNAMLQA.............P. Murexchinus melanurus
 EF028743 ..V.........................V.TEEDLAAKLNAMLQA.............P. Micromurexia habbema
 EU086688 ..V.........................V.TEEDLAAKLNAMLQA.............P. Pseudantechinus macdonnellensis
 EU086689 ..V.........................V.TEEDLAAKLNAMLQA.............P. <font color="magenta">Pseudantechinus roryi</font>
 EU086686 ..V.........................V.TEEDLAAKLNAMLQA............SP. Pseudantechinus macdonnellensis
 EU086687 ..V.........................V.TEEDLAAKLNAMLQA..........G..P. Pseudantechinus mimulus
 AY532667 ..V.........................V.TEEDLAAKLNAMLQA.............P. Murexia longicaudata
 EF028746 ..V.........................V.TEEDLAAKLNAMLQA.............P. Paramurexia rothschildi
 AY532677 ..V.........................V.TEEDLAAKLNAMLQA.............P. Dasyuroides byrnei
 EF028745 ..V..........I..............V.TEEDLAAKLNAMLQA.............P. Phascomurexia naso
 
 Macropus eugenii assembly         
 sacHar   MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE 
          ME+LQ YYTLVDRVPALLHHLTAIDYSS L  +   ++       VSEDPRLLVRVLR E
 macEug   MEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPE  
 
 Monodelphis domestica assembly     TSSLVLDLQHSSGGEISG 
 sacHar   MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE  
          ME+LQ YYTLVDRVPALLHHLTAIDYSS L  +   ++       VSEDPRLLVRVLR E
 monDom   MEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPE  
 
 Ornithorhynchus anatinus assembly
 sacHar    EILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE 
           ++L+ YY LVDRVPALL HL A+D SS L  +   SR        SEDPRLLVR L  E
 ornAna    DLLRDYYALVDRVPALLRHLAALDLSSVLSEEDLTSRLNAGLQAASEDPRLLVRRLEPE  
 
 Equus caballus assembly
 sacHar    EILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE  
           E LQ YYTLVDRVPALLHHL ++D+SS +  D   ++       VSEDPRLLV V+RS+
 equCab    EALQDYYTLVDRVPALLHHLASMDFSSVVSEDDLVAKLNAGLQAVSEDPRLLVWVVRSK

=== Rod rhodopsin RHO1 (4+ marsupials) ===

The optimal wavelength for scotopic (dim light) vision of Sarcophilus is easily predictable provided key tuning residues are covered by the assembly. The 97% match to Sminthopsis and agreement at tuning residues suggests this aspect of vision will be nearly identical between the two species.

<pre>
>RHO1_homSap Homo sapiens (human)   
0 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 1
2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSR 2
1 YIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQ 0
0 FRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA* 0

>RHO1_monDom Monodelphis domesticus (opossum) Didelphimorphia
0 MNGTEGPNFYVPFSNKTGTVRSPFEEPQYYLADPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTMTLYTSLHGYFVFGPTGCNLEGFFATLG 1
2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIIGVAFTWVMALACAFPPLIGWSR 2
1 YIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPLIVIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPIFMTIPAFFAKSSSVYNPVIYIMMNKQ 0
0 FRTCMITTLCCGKNPLGDDEASATASKTETSQVAPA* 0

>RHO1_macEug Macropus eugenii (wallaby) Diprotodontia frag, traces not yet consulted
0 MNGTEGPNFYVPFSNKTGVVRSPFEEPQYYLAEPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLADADLFMDFGGFT       1
2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACSTPPLLGWSR 2
1 0
0       ESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTLPAFFAKTSAVYNPVIYIMMNKQ 0
0 FRNCMITTLCCGKNPLGDDEASATTSKTETSQVAPA* 0

>RHO1_smiCra Sminthopsis crassicaudata (fat-tailed dunnart) Dasyuromorphia
0 MNGTEGPNFYVPYSNKSGVVRSPYEEPQYYLAEPWMFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAVADLFMVICGFTTTLVTSLNGYFVFGTTGCLVEGFFATTG 1
2 GEVALWALVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWIMALACSVPPIFGWSR 2
1 YIPEGMQCSCGIDYYTLNPEFNNESFVIYMFVVHFIIPLTVIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQ 0
0 FRNCMITTLCCGKNPLGDDEASTTASKTETSQVAPA* 0

>RHO1_sacHar Sarcophilus harrisii (tasmanian_devil) 97% identity Sminthopsis crassicaudata
0 MNGTEGPNFYVPHSNKTGVVRSPYEEPQYYLAEPWMFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAVADLFMVICGFTTTLVTSLNGYFVFGTTGCQIEGFFATTG 1
2 GEVALWALVVLAIERYIVVCKPMSNFRFGENHAIMGVVFTWIMALACSVPPLFGWSR 2
1 YIPEGMQCSCGIDYYTLNPEFNNESFVIYMFVVHFTIPLTVIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQ 0
0 FRTCMITTLCCGKNPLGDDEASATVSKTETSQVAPA* 0

>RHO1_calPhi Caluromys philander (woolly opossum) Didelphimorphia abstract:14659889
0 MNGTEGPNFYVPFSNKTGVVRSPFEEPQYYLAEPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTTTLYTSLHGYFVFGPTGCDLEGFFATLG 1
2 GEIALWSLVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSR 2
1 YIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMVVIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPILMTLPAFFAKTSAVYNPVIYIMLNKQ 0
0 FRTCMLTTLCCGKIPLGDDEASATASKTETSQVAPA*

>RHO1_ornAna Ornithorhynchus anatinus (platypus) 
0 MNGTEGQDFYIPMSNKTGVVRSPFEYPQYYLAEPWQYSVLAAYMFMLIMLGFPINFLTLYVTIQHKKLRTPLNYILLNLAFANHFMVLGGFTTTLYTSLHGYFVFGPTGCNIEGFFATLG 1
2 GEIALWSLVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWIMALACALPPLVGWSR 2
1 YIPEGMQCSCGIDYYTLRPEVNNESFVIYMFVVHFTIPMTIIFFCYGRLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTVPAFFAKSSAIYNPVIYIMMNKQ 0
0 FRNCMLTTICCGKNPLGDDEASATASKTEQSSVSTSQVSPA* 0

Cone rhodopsin SWS2 (9+ marsupials)

Cone rhodopsin RHO2 has been lost in all mammals and no debris from this gene is expected in Sacrophilus). The short wavelength cone opsin SWS2, while still present in platypus, has also been lost in all theran opsin too long ago to leave detectable remnants in syntenic position. Cone opsin SWS1 has this turned around, being present in theran mammals but only as debris in platypus. A nearly full length gene, most simiilar to Sminthopsis, can be recovered from Sarcophilus read coverage.

>SWS1_homSap Homo sapiens (human) Gt -FAM137A -CALU -NAG6 -FLNC 1385866 NP_990769 cone short   
0 MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVA 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSR 2
1 FIPEGLQCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNPIIYCFMNKQ 0
0 FQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN* 0

>SWS1_monDom Monodelphis domesticus (opossum) Didelphimorphia
0 MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHFQTVFMGFVFCAGTPLNAVVLVATLRYKKLRQPLNYILVNVSLCGFIFCIFAVFTVFISSSQGYFIFGRHVCAMEAFLGSVA 1
2 GLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWVIGIGVSIPPFFGWSR 2
1 FIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIMPLFLICFSYSQLLRALRA 0
0 VAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNQNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0
0 FHACIMEMVCRKPMTDDSDVSSSQKTEVSAVSSSQVGPT* 0

>SWS1_thyEle Thylamys elegans (fat-tailed opossum) Didelphimorphia 
MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHLQTVFMGFVFC
AGTPLNAVVLVATLRYKKLRQPLNYILVNVSFSGFIFCIFAVFTVFISSSQGYFIFGH
HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWVIGI
GVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLFLICFSY
SQLLGALRAVAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNRNH
GLDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMVCRKPMTDDSDVSSSQKTE
VSAVSSSQVGPS

>SWS1_didAur Didelphis aurita (big-eared opossum) Didelphimorphia
MSGDEEFYLFKNISSVGPWDGPQHHIAPAWAFHFQTVFMGFVFC
AGTPLNAVVLVATLRYKKLRQPLNYILVNVSLSGFIFCIFAVFTVFISSSRGYFVFGR
HVCAMEAFLGSVAGLVMGWSLAFLAFERFVVICKPFGNFRFNAKHAMMVVLATWVIGI
GVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYAWFLFLSCFIGPLFLICFSY
AQLLGALRAVAAQQQESTTTQKAEREVSRMVVMMVGSFCLCYVPYAALGMYMINNRNH
GLDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMVCRKPMADDSDITSSQKTE
VSTVSSSQVGPS

>SWS1_macEug Macropus eugenii (wallaby) Diprotodontia
MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHCQTVFMGFVFFAGTPLNAVVLIATFRYKKLRQPLNYILVNISLAGFIYCIFSVFTVFISSSQGYFIFGR
HVCAMEGFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFNSKHSMMVVLATWVIGIGVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFILCFIMPLSLICFSY
SQLLGALRAVAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNRNHGIDLRLVTIPAFFSKSSCVYNPIIYCFMNKQFHACIMEMVCRKPMTDDSEASSSQKTEVSTVSSSQVGPS*

>SWS1_setBra Setonix brachyurus (quokka) Diprotodontia 
MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHCQTVFMGFVFF
AGTPLNAVVLIATFRYKKLRQPLNYILVNISLAGFIYCIISVFTVFISSSQGYFIFGR
HVCAMEGFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFNSKHSMMVVLATWVIGI
GVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYHSEYYTWFLFILCFIMPLSLICFSY
SQLLGALRAVAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNRNH
GIDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMVCRKPMTDDSEASSSQKTE
VSTVSSSQVGPS

>SWS1_tarRos Tarsipes rostratus (honey possum) Diprotodontia
MSGDEEFYLFKDISSVGPWDGPQYHIAPAWAFHFQTTFMGFVFF
AGTPLNAVVLIATLRYKKLRQPLNYILVNISLAGFIFCVISVFTVFISSSQGYFIFGR
HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFSSKHAMMVVLATWVIGI
GVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYHSEYYTGFLFIFCFIVPLSLICFSY
SQLLGALRAVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNH
GLDLRLVTIPAFFSKSACVYNPIVYWFMNKQFHACIMEMVCRKPMTDDSEISSSQKTE
VSTVSSSQVGPS

>SWS1_cerCon Cercartetus concinnus (pygmy possum) Diprotodontia
MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHFQTAFMGFVFF
VGTPLNAVVLVATLCYKKLRQPLNYILVNVSLAGFIFCIISVFTVFISSSQGYFIFGR
HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFSSKHAMMVVLATWVIGI
GVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLICFSY
SQLLGALRAVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNH
GLDLRLVTIPACFSK 

>SWS1_smiCra Sminthopsis crassicaudata (dunnart) Dasyuromorphia
0 MSGDEEFYLFKNISLVGPWDGPQYHLAPAWAFHFQTAFMGFVFFAGTSLNGVVLIATLRYKKLRQPLNYILVNISLAGFIFCVFSVFTVFVSSSQGYFVFGRHVCAMEGFLGSVA 1
2 GLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWIIGIGVSIPPFFGWSR 2
1 YIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLICFSYSQLLGALRA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAAMAMYMVNNRNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0
0 FHACIMEMICKKPMTDDSETTSSQKTEVSTVSSSQVGPS* 0

>SWS1_sacHar Sarcophilus harrisii (tasmanian_devil) part of last exon missing 96% identity Sminthopsis crassicaudata
0 MSGDEEFYLFKNISPVGPWDGPQYHIAPAWAFHLQTAFMGFVFFAGTPLNGVVLIATLRYKKLRQPLNYILVNISLAGFIFCVFSVFTVFVSSSQGYFVFGRHVCAMEGFLGSVA 1
2 SGLVTGWSLAFLAFERFIVICKPFGNFRFHSKHATMVVLATWVIGIGVSIPPFFGWSR 2
1 YIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLICFSYSQLLGALRAVS 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNQ 0
0            KPMTDDSETTSSQKTEVSTVSSSQVGPS* 0
 
>SWS1_isoObe Isoodon obesulus (bandicoot) Peramelemorphia
MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHCQTVFMGFVFF
AGTPLNAVVLIATLRYKKLRQPLNYILVNISLAGFIFCIFSVFTVFISSSQGYFIFGR
HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFHSKHAMMVVLATWVIGI
GVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIIPLSLICFSY
SQLLRALRTVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNH
GLDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMICRKPMTDDSETSSSQKTE
VSTVSSSQVSPS

>SWS1_galGal Gallus gallus (chicken) Gt 0...2.1.0.0 indel x x x x 348 aa 000 nm no_ref genome cone short1 violet   
0 MSSDDDFYLFTNGSVPGPWDGPQYHIAPPWAFYLQTAFMGIVFAVGTPLNAVVLWVTVRYKRLRQPLNYILVNISASGFVSCVLSVFVVFVASARGYFVFGKRVCELEAFVGTHG 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFSSRHALLVVVATWLIGVGVGLPPFFGWSR 2
1 YMPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0
0 FRACIMETVCGKPLTDDSDASTSAQRTEVSSVSSSQVGPT* 0

Cone rhodopsin LWS (9+ marsupials)

This basal long wavelength imaging opsin is available from 97 vertebrates and has already been analyzed for phyloSNPs and rare genomic events. The Didelphimorphia experienced a 3-4 residue insert in exon 1 that separates them from all other marsupials. Note this region has quite a complicated indel history. The extra residues have repeat character DVNE DDND suggesting replication slippage. The gene is present and intact in Sarcophilus though two exons are not currently available. LWS in tasmanian devil is identical to the Sminthopsis ortholog.

LWS_loxAfr  MAQQWGPHRLTGARLQDASE---DSTQASIFVYTNTNT  elephant
LWS_echTel  MAQRWGAHRLTGGQLQDTYE---GSTRTSIFVYTNSTS  tenrec
LWS_monDom  MTQAWDPAGFLARRRDVNEDDNDETTRSSLFVYTNSNN Didelphimorphia
LWS_didAur  MTQAWDPVGFLARRRDENEDDHDDTTRASLFVYTNSNN Didelphimorphia
LWS_tarRos  MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Diprotodontia
LWS_macEug  MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Diprotodontia
LWS_smiCra  MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Dasyuromorphia
LWS_sacHar  MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Dasyuromorphia
LWS_setBra  MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Diprotodontia
LWS_cerCon  MTQAWDPAGFLAWQEDENE----ETTRASLFVYTNSNN Diprotodontia
LWS_myrFas  MTQAWDPAGFLAWRREENE----ETTRASLFTYTNSNN Dasyuromorphia
LWS_isoObe  MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Peramelemorphia
LWS_ornAna  MTPAWNSGVYAARRRFEDEE---DTTRTSVFVYTNSNN  platupus
LWS_tacAcu  MTQAWDPAGFLAWRRDENEE---TTRASLFVYTNSNNT  echidna

>LWS_homSap Homo sapiens (human)   
0 MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTR 1
2 GPFEGPNYHIAPRWVYHLTSVWMIFVVIASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISVVNQVYGYFVLGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWMVVCKPFGNVRFDAKLAIVGIAFSWIWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCITPLSIIVLCYLQVWLAIRA 0
0 VAKQQKESESTQKAEKEVTRMVVVMVLAFCFCWGPYAFFACFAAANPGYPFHPLMAALPAFFAKSATIYNPVIYVFMNRQ 0
0 FRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA* 0

>LWS_monDom Monodelphis domesticus (opossum) Didelphimorphia 4aa insert  
0 MTQAWDPAGFLARRRDVNEDDNDETTRSSLFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETVIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAAVWTAPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMATCCIFPLSIILLCYVQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYSFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSKTEGSSVSSVAPA* 0

>LWS_didAur Didelphis aurita (big-eared opossum) Didelphimorphia 4aa insert
0 MTQAWDPVGFLARRRDENEDDHDDTTRASLFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETVIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAAVWTSPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDLGVQSYMIVLMATCCIFPLSIILLCYIQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSKTEVSSVSSVAPA* 0

>LWS_tarRos Tarsipes rostratus (honey possum) Diprotodontia ENED insert
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVFNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAIWTSPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGNSDPGIQSYMIVLMSTCCILPLSIILLCYVQVWRAIRA 2
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0

>LWS_macEug Macropus eugenii (wallaby) Diprotodontia  
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1
2 GPFEGPNYHIAPRWVFNLTSLWMIFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGNSDPGVQSYMIVLMSTCCILPLSVIFLCYIQVWLAIRS 2
0 VAKQQKESESTQKAEKEVSRMVVVMILAFCFCWGPYAIFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0

>LWS_setBra Setonix brachyurus (quokka) Diprotodonti  
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1
2 GPFEGPNYHIAPRWVFNLTSLWMIFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETMIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGNSDPGVQSYMIVLMSTCCILPLSVILLCYIQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYAIFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVPA* 0

>LWS_cerCon Cercartetus concinnus (pygmy possum) Diprotodontia 
0 MTQAWDPAGFLAWQEDENEETTRASLFVYTNSNNTK 1
2 GPFEGPNYHIAPRWVFNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAIADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAIWTSPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGNSDPGIQSYMIVLMSTCCILPLSIILLCYIQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTFFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA*

>LWS_smiCra Sminthopsis crassicaudata (dunnart) Dasyuromorphia
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1
2 GPFEGPNYHIAPRWVYNLTSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIILCYIQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVMVMILAFCFCWGPYALFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0

>LWS_sacHar Sarcophilus harrisii (tasmanian_devil) half of exon 2, all of exon 4 missing frag 100% identical to Sminthopsis
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1
2                                            FKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIILCYIQVWLAIRA 0
0 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0

>LWS_myrFas Myrmecobius fasciatus (numbat) Dasyuromorphia
0 MTQAWDPAGFLAWRREENEETTRASLFTYTNSNNTK 1
2 GPFEGPNYHIAPRWVYNLTSFWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSVILLCYIQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYAIFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0

>LWS_isoObe Isoodon obesulus (bandicoot) Peramelemorphia  
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNLTSFWMFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMTTCCILPLSIILLCYVQVWLAIRA 0
0 VAKQQKDSESTQKAEKEVSRMVVVMIRAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSGTSRTEVSSVSSAPA* 0

>LWS_ornAna Ornithorhynchus anatinus (platypus)  
0 MTPAWNSGVYAARRRFEDEEDTTRTSVFVYTNSNNTR 1
2 DPFEGPNYHIAPRWAYNVTSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIFGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLSIISWERWIVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIVLCYLQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTIFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCIMQLFGKKVDDGSELSSTSRTEVSSVSSVSPA* 0

>LWS_tacAcu Tachyglossus aculeatus (echidna)  
0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVFNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAVWTSPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMATCCIFPLSIILLCYIQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSKTEVSSVSSVAPA* 0

Encephalopsin (2+ marsupials)

Pinopsin, parapinopsin, parietopsinand VA opsin all terminate in sauropods and are missing in all mammals. Encephalopsin has a very peculiar history of gene loss in tetrapods, requiring some seven independent and asynchronous events including platypus. While this limits the phylogenetic utility of any gene loss within marsupials, the status of the gene within Sarcophilus is still informative. A full length gene can be recovered with 94% identity to opossum, strongly indicating that encephalopsin is fully functional within Sarcophilus.

>ENCEPH_homSap Homo sapiens (human) OPN3 
0 MYSGNRSGGHGYWDGGGAAGAEGPAPAGTLSPAPLFSPGTYERLALLLGSIGLLGVGNNLLVLVLYYKFQRLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLF 1
2 GIVSIATLTVLAYERYIRVVHARVINFSWAWRAITYIWLYSLAWAGAPLLGWNRYILDVHGLGCTVDWKSKDANDSSFVLFLFLGCLVVPLGVIAHCYGHILYSIRM 0
0 LRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVNGHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRK 0
0 FRRSLLQLLCLRLLRCQRPAKDLPAAGSEMQIRPIVMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVDDSDKTNGSKVDVIQVRPL* 0

>ENCEPH_monDom Monodelphis domestica (opossum) 
0 MYSDNSSDDGGGGYWGSGRAGGASGTGVTGEPGPEGSPRQAPLFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFNDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF 1
2 GIVSIMTLTVLAYERYNRIVHAKVINFSWAWRAITYIWLYSLVWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVIFLFFGCLMLPVGVMAYCYGHILYAIRM
0 LRCVEELQTIQVIKILRYEKKVAKMCFLMIAIFLFCWMPYAVICLLVANGYGSLVTPTVAIIASLFAKSSTAYNPIIYIFMSRK 0
0 FRRCLLQLLCFRLLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDENDKNSGTKVNVIQVRPL* 0

>ENCEPH_sacHar Sarcophilus harrisii (tasmanian_devil) 94% identity monDom
0 MYSGNSSDDAGGGYWGSGGTGGAGGTGVAGEPAPEGSPRPAPLFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFSDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF 1
2 GIVSIMTLTVLAYERYNRIVHAKVINFSWAWRAITYIWLYSLIWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVLFLFLGCLVLPVGVMAYCYGHILYAIRM 0
0 FRCVEELQTLQVIKILRYEKKVAKMCFLMIATFLFCWMPHAVICFLVANGYGSLVTPTVAIIPSLFAKSSTAYNPIIYIFMSRK 0
0 FRRCLLQLLcFRQLKFQQPKKDRAIIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDDNDKNSETKVNVIQVRPL* 0

>ENCEPH_macEug Macropus eugenii frag
0                         GALGCREPGQREPSSSAPFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLLLVNISFSDLLVSLFGVTFTFVSCLRSGWVWHTVGCAWDGFSNSLF 1
2 GIVSIMTLTVLAYERYHRIVHAKVINFSWTWRAITYIWLYSLVWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVLFLFLGCLVLPVGVMAYCYGHILYAIRM 0
0 0
0 FRRCLLQLLCFRQLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDDNDKNNGTKVNVIQVRPL* 0

>ENCEPH_ornAna Ornithorhynchus anatinus pseudo
0 MVPWNGS-GRHLGAVR---GPE--SLPATPGAARPSRPGAGDGRL--LGLF-P-GVGGNLLVLLL--ALPGPPTTTDLYLASVAVSDLL--LL---LPFVYRLWRSRPWVFVCRLLGE-GGSLA 1
2 GIVSLISLAVLSYERYTLTLHPKQSNYQKAVLAVGASWIYSLIWTIPPLLGWSSYGTEGAGTSCSVHWSSKSVC-SYIVCLFI--CLVIPVLVMIYCYGRLLYAVKQ 0
0 LHCVKELQNIQVIGSLRYER*VTEMYFFTIAQFLVCQSPSALVSYPAAH-----VSPVVAKISPVFANSSFVYNPVISIFVRRK 0
0 KASR*KVNVIQVQPPS* 0

>ENCEPH_galGal Gallus gallus (chicken) 71%=homSap encephalopsin OPN3 full
0 MHSGNGTGATSRPQLAAAGHEVPGERPLFSAGTYELLALLIATIGTLGVCNNLLVLVLYYKFKRLRTPTNLFLVNISLSDLLVSVCGVSLTFMSCLRSRWVWDAAGCVWDGFSNSLF 1
2 GIVSIMTLTVLAYERYIRVVHAKVIDFSWSWRAITYIWLYSLAWTGAPLLGWNRYTLEIHGLGCSMDWKSKDPNDTSFVLLFFLGCLVAPVVIMAYCYGHILYAVRM 0
0 LRCVEDFQTSQVIKLLKYEKKVAKMCFLMISTFLICWMPYAVVSLLVTYGYSNLVTPTVAIIPSFFAKSSTAYNPVIYIFMSRK 0
0 FRQCLLQLLCFRLMRFQRIMKEPSGAGNVKPIRPIVMSQKVGDRPKKKVTFSSSSIIFIIASDDTQQIDDNSKHNGTKVNVIQVKPL* 0

TMT opsin (2+ marsupials)

TMT is an ancient locus that is present in monotremes and marsupials but lost in all placentals.

>TMT_monDom Monodelphis domestica shortened final exon 
0 MSNNLTTNLSLEALLSASEDKQRNGLSRTGHTIVAVFLGIILIFGSISNFIVLVLFCKFKVLRNPVNMLLLNISISDMLVCLSGTTLSFASSIQGRWIGGKHGCRWYGFANSCF 1
2 GIVSLISLAILSYERYRTLTLCPGQGADYQKALLAVAGSWLYSLVWTVPPLIGWSSYGTEGAGTSCSVHWTSKSVESVSYIMCLFIFCLVIPILVMVYFYGRLLYAVKQ 0
0 VGKIRKTAARKREYHVLFMVVTAVICYLICWVPYGMIALLATFGPPGVVSPVANVVPSILAKSSTVCNPIIYVLMNKQ 0
0 FYKCFLILFHCQPAQSGPDVSLCPSNVTVIQLGQRKNKDAPGSI*

>TMT_macEug Macropus eugenii frag
0 MSINLTANLSFGTLLPDSEEKQRSGLSRTGHTVTAVFLGLILILGVINNFIVLVLFCKFKVLRNPVNMLLLNISISDMLVCLTGTTLSFASSIRGRWIAGYHGCRWYGFANSCF 1
2 GIVSLISLAVLSYERYRTLTLCPRQGTDYHKALLAVAGSWLYSLIWTVPPLIGWSSYGTEGAGTSCSVHWTSKSVESVSYIMCLFIFCLVIPILFMVYFYGRLLYTVKQ 0
0 VGKIRKSAARKREYHVLFMVVTAVICYLICWVPYGMIALLATFGPPGVVSPVANVVPSILAKSSTVCNPIIYILMNKQ 0
0 FYKCFLILFHCQPASSASDASLCPSKMTVIQLGQRKDKEVPCAIQDLPEVSKKQLCLLSPESNVAPSSGHPQEKMEEKPLSE*  0

>TMT_sacHar Sarcophilus harrisii (tasmanian_devil) FP5MBH101BETOZ needed to finish
0 MSINLTTNLSFGPLLIDSEEKPRSGLSRTGHTVVAVFLGIILILGFINNFIVLILFCKFKVLRNPVNMLLLNISISDMLVCLSGTTLSFASSIRGRWIGGYHGCRWYGFANSCF 1
2 GIVSLISLAILSYERYRTLTLCPRRGADYQKALLAVAGSWLYSLIWTVPPLIGWSSYGTEGAGTSCSVHWTSKSVQSVSYIMCLFIFCLVIPILIMIYFYGRLLYTVKQ 0
0 VGKIRKTAARKREYHVLFMVVTAVICYLICWVPYGLIALVATFGPPGVVSPVANIVPSILAKSSTVCNPIIYILMNKQ 0
0 FYKCFLILFHCQPASSAPDASLCPSKVTVIQLGQR   * 0

>TMT_ornAna Ornithorhynchus anatinus frag
0                        GLSRTGHTMVAVFLGIILVFGFMNNLIVLILFCKFKALRNPVNMIMLNISASDMLVCVSGTTLSFASNISGRWIGGDPGCRWYGFVNSCL 1
2 GIVSLISLAVLSYERYRTLTLHPKQSTDYQKAVLAVGASWIYSLIWTIPPLLGWSSYGTEGAGTSCSVHWSSKSPVSVSYIVCLFIFCLVIPVLVMIYCYGRLLYAVKQ 0
0 IGKARKTAARKREYHVLFMVITTVICYLVCWMPYGVTALLATFGQPGTVSPEASVIPSILAKSSTVCNPIIYILMNKQ 0
0 FYKCFLILFHCQPPRAADAPSTYPSQVMVIQLNQRRSRETAGAPQVLLEMKHQTLHLLGPQLHETPSWERSTPVHPE* 0

>TMT_galGal Gallus gallus 
0 MNHTWTYNLSFGAPTDPVEPRAGLSRNGHTVVAVFLGFILFFGFLNNLIVLILFCKFKTLRNPVNMLLLNIhttp://genomewiki.ucsc.edu/index.php?title=Opsin_evolution:_Encephalopsin_gene_loss&action=editSISDMLVCISGTTLSFASNIHGKWIGGEHGCRWYGFVNSCF 1
2 GIVSLISLAVLSYERYSTLTLCNKRSDDYRKALLAVGGSWVYSLLWTVPPLLGWSSYGIEGAGTSCSVRWSSETAESTSYIICLFIFCLVIPVMVMMYCYGRLLYAVKQ 0
0 VGKIHKNTARKREYHVLFMVITTVICYLVCWIPYGVIALLATFGKPGVVTPVASIIPSILAKSSTVCNPIIYILMNKQ 0
0 FYKCFRQLFHCQPPSSTDGEPTCHSKVTVIQLNQKTDGGKLCNNKPRPETDNKVTSLLHPEPGLEPAAKTVPPM*  0

>TMT_taeGut Taeniopygia guttata 
0 MNHTWMYNLSFGAPAHPVEPRAGLSRSGHTVVAVFLGLILFFGFLNNLIVLILFCKFKTLRNPVNMLLLNISVSDMLVCISGTTLSFASNIRGKWIGGDHACRWYGFVNSCF 1
2 GVVSLISLAVLSYERYNTLTLCHKRSDDFRKALLAVAGSWIYSLVWTVPPLLGWSSYGVEGAGTSCSVRWSSESAESTSYIICLFVFCLVVPVMVMMYCYGRLLYAVKQ 0
0 VGKIHKNAARKREYHVLFMVIPTVICYLVCWIPYGVIALLATFGKPGAVTPITSIIPSILAKSSTVCNPIIYILMNKQ 0
0 FYKCFRQLFHCQPPSSTDGEPTCHSKVTVIQLDQRADGGNMCNNEPHPETDSKMTSLLCPETTSKATPPTS* 0

>TMT_anoCar full +TMT -ST6GAL2 (overlap) +SLC5A7
0 MSELSSNLTFNMSTSIEEPGSGLSRMGHNIVAVFLGLILVFGFLNNLVVLILFCKFKTLRNPVNMLLLNISASDMLVCISGTTLSFVSNIYGRWIGGEHGCRWYGFVNSCF 1
2 GIVSLISLAILSYERYSTLTQTNKRGSDYQKALLGVGGSWLYSLIWTVPPLIGWSSYGLEGAGTSCSVRWTSETLESVTYIICLFIFCLAIPVLVMIYCYARLFYAVKQ 0
0 VGKLRKTSARKREFHVLFMIITTIICYLICWMPYGVIALLATFGRPGLVSPVASVIPSILAKSSTVFNPIIYILMNKQ 0
0 FYKCFLMLLHCQPSSVADGETICQSKVMAIHQNQKAQGGVILKSQVVPQMDEKAICLLSPESSLDPVLESTPQLSKENSFL* 0

>TMT_xenTro full -UXS1 +TMT -ST6GAL2 (overlap) +SLC5A7
0 MSTIKNWTTNISVENSMSYIENDLSLPTEAVLSRTGHTVVAIFLGFILIFGFLNNFVVLILFCKFKTLRTPVNMMLLNISASDMLVCVSGTTLSFTSSIKGKWIGGEYGCQWYGFVNSCF 1
2 GIVSLISLAILSYERYSTLTLYNKGGPNFKKALLAVASSWLYSLVWTVPPLLGWSSYGREGAGTSCSVRWTSESVESVSYIICLFIFCLALPVFVMLYCYGRLLYAVKQ 0
0 VGKIRKIAARKREYHVLFMVITTVICYLLCWLPYGVVALLATFGRPGVISPVASVVPSILAKSSTVFNPIIYILMNKQ 0
0 FYKCFLILFHCHPTSSADGKSICQSNYTVIQLNQKLNNIVAIPGQTQIPESVDKMPCIHRQNNESPSDQMPQSTTEHLISGT* 0

RGR opsin (0 marsupials)

This gene has apparently been lost specifically in the marsupial clade, though support for that is only provided by the Monodelphis and Macropus genome projects. It would be of considerable interest to find the gene or a fragment thereof in syntenic position in Sarcophilus. However nothing can be found with tblastn of current reads.

>RGR1_homSap Homo sapiens (human) +PCDH21 -LRIT1 -GRID1 -WAPAL NM_001012720 retinal epithelium Mueller    
0 MAETSALPTGFGELEVLAVGMVLLVE 1
2 ALSGLSLNTLTIFSFCKTPELRTPCHLLVLSLALADSGISLNALVAATSSLLR 2
1 RWPYGSDGCQAHGFQGFVTALASICSSAAIAWGRYHHYCT 1
2 RSQLAWNSAVSLVLFVWLSSAFWAALPLLGWGHYDYEPLGTCCTLDYSKGDR 2
1 NFTSFLFTMSFFNFAMPLFITITSYSLMEQKLGKSGHLQ 0
0 VNTTLPARTLLLGWGPYAILYLYAVIADVTSISPKLQM 0
0 VPALIAKMVPTINAINYALGNEMVCRGIWQCLSPQKREKDRTK* 0

>RGR_dasNov Dasypus novemcinctus (armadillo) 
0 MAGSGVLPPGFGELEVLAVGTVLLVE 1
2 ALSGLVLNGLAIISFCKTPELRSPSRLLVLSLALADSGVSLNALVAATSSLLR 2
1 RWPYGSGGCQAHGFQGFVTALASISSSAAIAWERCHRHCI 1
2 GRRLAWSTAGCLVLCLWMAAAFWAALPLLGWGLYDYEPLGTCCTLDYSRGDR 2
1 NFISFLVTLALFNFFLPLLIMLTSYRLMAQKLKRSGHVQ 0
0 VSTALPGRLLLLGWGPYALLYLYAAVADATSLSPRLQM 0
0 VPALIAKTMPTVNALYYALGRESVHRNA* 0

>RGR_loxAfr Loxodonta africana (elephant) 
0 MAEPGHLPAGFQELEVLTVGTVLLLE 1
2 ALSGLSLNGLTILSFCKIPELRTPGHLLVLSLALADSGISLNALVAAMSSLRR 2
1 RWPYGSDGCQAHGFQGFVTALASICSCAAIAWERYHHYCT 1
2 RSRLAWSSASALVLFVWLSSAFWAALPLLGWGRYNYEPLGTCCTLDYSRGDR 2
1 NSTSFLLTMAFFNFLLPLFITLTSYRLMEQKLKKKGPLQ 0
0 VNTTLPARTLLLGWGPYALLYLCAAATDMTSISPRLQM 0
0 VPALVAKAVPVINACHYALGSEVVRGGIWQYLSRQRGESPLRARDRTH* 0

>RGR1_ornAna Ornithorhynchus anatinus (platypus) missing exon 1 DRY motif, afros ERY, other placentals GRY
0 1
2 ALLGLCLNGLTIASFRKIKELRTPSNLLVVSLALADSGICLNALMAALSSFLR 2
1 HWPYGAEGCRLHGFQGFATALASISLSAAIGWDRYLRHCS 1
2 RSKPQWGTAVSTVLFAWGFSAFWSMMPILGWGQYDYEPLRTCCTLDYSKGDR 2
1 NFTTYLFAVAFFNFVIPLFIMLTSYQSIEQRFKKSGLFK 0
0 LNTRLPTRTLLFCWGPYALLCFYATVENVTFISPKLRM 0
0 IPALIAKTVPVIDAFTYALRNEDYRGGIWQFLTGQKIERVEVENKIK* 0

>RGR1_galGal Gallus gallus (chicken) +PCDH21 -LRIT1 +CHAT -PARG 14985289 NM_001031216  
0 MVTSHPLPEGFTEIEVFAIGTALLVE 1
2 ALLGFCLNGLTIISFRKIKELRTPSNLLVLSIALADCGICINAFIAAFSSFLR 2
1 YWPYGSEGCQIHGFQGFLTALASISSSAAVAWDRYHHYCT 1
2 RSKLQWSTAISMMVFAWLFAAFWATMPLLGWGEYDYEPLRTCCTLDYSKGDR 2
1 NYITFLFALSIFNFMIPGFIMMTAYQSIHQKFKKSGHYK 0
0 FNTGLPLKTLVICWGPYCLLSFYAAIENVMFISPKYRM 0
0 IPAIIAKTVPTVDSFVYALGNENYRGGIWQFLTGQKIEKAEVDSKTK* 0

>RGR1_xenTro Xenopus tropicalis (frog) ?? 0.2.1.2.1.0.0 indel +PCDH21 -LRIT1 +CHAT -PARG 296 BC135113  
0 MVTSYPLPEGFTETEVFAIGTTLLVE 0
0 ALLGLLLNGLTLLSFYKIRELRTPSNLFIISLAVADTGLCLNAFVAAFSSFLR 2
1 YWPYGSEGCQIHGFQGFVAALSSIGSCAAIAWDRYHQYCT 1
2 RSKLHWSTAVSVVFFIWGFSAFWSAMPLFGWGEYDYEPLRTCCTLDYSKGDR 2
1 NYISYLFTMAFFEFLVPLFILMTAYQSIYQKMKKSGQIR 0
0 FNTSMPVKSLVFCWGPYCLLCFYAVIQDATILSPKLRM 0
0 IPALLAKTSPAVNAYVYGLGNENYRGGIWQYLTGQKLEKAETDNKTK* 0

Peropsin (2+ marsupials)

Sarophillus can be expected to have this gene. Further, the protein sequence should substantiate the 4 previously defined phyloSNPs characteristic of the marsupial/placental transition.

>PER_homSap Homo sapiens (human)
0 MLRNNLGNSSDSKNEDGSVFSQTEHNIVATYLIMA 1
2 GMISIISNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQ 0
0 VYAGLNIFFGMASIGLLTVVAVDRYLTICLPDV 1
2 GRRMTTNTYIGLILGAWINGLFWALMPIIGWASYAPDPTGATCTINWRKNDR 2
1 SFVSYTMTVIAINFIVPLTVMFYCYYHVTLSIKHHTTSDCTESLNRDWSDQIDVTK 0
0 MSVIMICMFLVAWSPYSIVCLWASFGDPKKIPPPMAIIAPLFAKSSTFYNPCIYVVANKK 2
1 FRRAMLAMFKCQTHQTMPVTSILPMDVSQNPLASGRI* 0

>PER_loxAfr Loxodonta africana (elephant)
0 MLRNSLDNSSDSKNEDASVFSQTEHNIVATYLIMA 1
2 GMISILSNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLHGRWKFGYTGCQ 0
0 IYAGLNIFFGMASIGLLTVVAVDRYLTICHPHI 1
2 GRRMTSNTYVSMILGAWINGLLWALLPITGWASYAPDPTGATCTINWRKNDA 2
1 SFVSYTMTVIVINFVVPLAVMFYCYYHVTRSIKRHTASNCAEYLNRDWSDQLDVTK 0
0 MSVIMILMFLVAWSPYSIVCLWASFGDSKKIPPSMAIIAPLFAKSSTFYNPCIYVVANKK 2
1 FRRAMFAMFKCQTHQAEPVTCILPMNVSQNPLAAGRI* 0

>PER_monDom Monodelphis domestica (opossum)
0 MFKNNSVKTLAPEKEGPSVFSPIEHKIVAAYLITA 1
2 GVISIVSNVIVLGIFVKYKALRTATNTIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYDGCQ 0
0 IYAGLNIFFGMASIGLLTAVAIDRYLTICQPDL 1
2 GGRMTSYNYTLMILTAWVNGFFWALMPIVGWAGYAPDPTGATCTINWRKNDV 2
1 SFVSYTMTVITINFAMPLGVMFYCYYNVSQKMKQYSPSNCPDHINRDWSNQVAVTK 0
0 MSVVMILMFLLAWSPYSIVCLWASFGDPKEIPPAMAIVAPLFAKSSTFYNPCIYVAANKK 2
1 FRRAISAMIRCQTHQSMPISNALPMN* 0

>PER_macEug Macropus eugenii (wallaby)
0 MFQNDSLEPEKESYSVFSPTEHNIVAAYLITA 1
2 GVISIPSNIIVLGIFVKYKELRTATNTIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQ 0
0 IYAGLNIFFGMASIGLLTAVAIDRYLTICQPDL 1
2 2
1 SFVSYTMTVIAINFVMPLVVMFYCYYNVSLKMKQYTRSSCPEHINRDWSNQVDVTK 0
0 MSVIMILMFLLAWSPYSVVCLWASFGDPKEIPPAMAIIAPLFAKSSTFYNPCIYVAANKK 2
1 FRRAISAMMRCETHQSMPVSNALPLNLT* 0

>PER_sarHar Sarcophilus harrisii (tasmanian_devil) 5.5 of 7 exons
0 MFKNDSFRSLEPEKEGHSVFSPAEHNIVAAYLITA 1
2   SILSNVIVLGIFVKFKELRTATNAIIINLA   0
0 1
2 GRRMTSFNYTIMILTAWVNGFFWALMPIVGWASYAPDPTGA   2
1 SFVSYTVTVIAINFVMPLVVMIYCYYNVSQKIKQYTPSNCPEYINRDWSNEVAVTK 0
0 MSVIMILMFLLAWSPYSVVCLWASFGDPKEIPPAMAIIAPLFAKSSTFYNPCIYVAANKK 2
1 FRrAISAMIQCQTHQSMSVSKALPMN* 0

>PER_ornAna Ornithorhynchus anatinus (platypus)
0 MRRNDSANLLESEHHDRSAFSQTDHNIVAAYLITA 1
2 GIMSIVSNVIVLGIFVKFEELRTATNAIIINLAVTDIGVSGIGYPMSAASDLHGSWKFGHAGCQ 0
0 IYAGLNIFFGMSSIGLLTVVAVDRYLTICRPAI 1
2 GRKMTRSNYTAMILAAWMNGFFWASMPLLGWASYASDPTGATCTINWRKNDA 2
1 SFISYTMTVIAVNFAVPLIVMFYCYYNVSKAMRQYPASRVLENLNIDWSEQVDVTK 0
0 MSVVMILMFLMAWSPYSIVCLWSSFGDPKKISPAVAIMAPLFAKSSTFYNPCIYVVANKK 2
1 FRRAMLSMVQCQTHREITITDVLPMNRSRSPLTL* 0

>PER_galGal Gallus gallus (chicken)
0 MHWNDSANSSESDAEAHSVFTQTEHNIVAAYLITA 1
2 GVISIFSNIVVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0
0 IYAALNIFFGMASIGLLTVVAVDRYLTICRPDI 1
2 GRRMTTRNYAALILAAWINAVFWASMPTVGWAGYASDPTGATCTANWRKNDV 2
1 SFVSYTMSVIAVNFVVPLTVMFYCYYNVSRTMKQYTSSNCLESINMDWSDQVDVTK 0
0 MSVVMIVMFLVAWSPYSIVCLWSSFGDPKKISPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRRAILAMVRCQTRQEITISNALPMTVSLSALTS* 0

>PER_taeGut Taeniopygia guttata (finch)
0 MHWNDSSNSSESDDEAHSAFTQTEHNIVAAYLITA 1
2 GVISIFSNIVVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0
0 IYAALNIFFGMASIGLLTVVAVDRYLTICRPDI 1
2 GRRMTTRSYATLILAAWINAVFWSSMPTAGWASYAPDPTGATCTVNWRKNDA 2
1 SFISYTMSVIAVNFVVPLTVMFYCYYNVSRTMKQYASSNCLESINIDWSDQVDVTK 0
0 MSVVMIIMFLVAWSPYSIVCLWSSFGDPKKISPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRRAILAMVRCQTRQEITINNALPMSVSQSALTSQNSSHLPA* 0

>PER_anoCar Anolis carolinensis (lizard)
0 MFLNDSANSSESDDEPHSAFSQAEHNIVAAYLITA 1
2 GVISLLSNIVVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0
0 IYAALNIFFGMASIGLLTVVAIDRYLTICKPHI 1
2 GSRLTATNYTTLILAAWINALFWASMPVVGWASYAPDPTGATCTVNWRKNDT 2
1 SFVSYTMSVIAVNFVIPLSVMFYCYYNVSKTMKYYMRNSCLENINIDWSDQVDVTK 0
0 MSVVMIIMFLLAWSPYSIVCLWSSFGDPKKISPAMAIVAPLFAKSSTFYNPCIYVIANKR 2
1 FRRAILAMIRCQTRQEITINNVLPMSVSQSTIA* 0

>PER_xenTro Xenopus tropicalis (frog)
0 METLAEVSTLLPAGTGTVNISDASSEVHSVFSQSEHNIVAAYLITA 1
2 GVISILSNIIVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYVGCQ 0
0 IYAGLNIFFGMASIGLLTVVAIDRYLTICRPDI 1
2 GRRISGRHYTAMILAAWINAVFWSVMPVVGWSSYAPDPTGATCTINWRKNDV 2
1 SFVSYTMSVVAVNFVVPLMVMFYCYYNVSRTMKGYGSRSSLGGINADWSDQTDVTK 0
0 MSMVMIVMFLVAWSPYSIVCLWSSFGDPRKIPPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRRAILSMVQCKSRQEVTLDNHFPMNVSQSTLTT* 0

Neuropsin (2+ marsupials)

Here Sarcophilus can be predicted to contain only NEUR1 because the ancient vertebrate genes NEUR2 and NEUR3 appear to terminate in sauropods and NEUR4 in platypus.

>NEUR1_homSap Homo sapiens (human) OPN5
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR1_dasNov
0 MALNHTALPQDDRLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 gILSTFGNGYVLYMSSKRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRATKKKSLEDFR 2
1 LHTVTTVRESSAVLEVHQEV* 0

>NEUR1_monDom
0 MALNHSVSPQDDYIPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKAAKKESLRTYR 2
1 LHTVTTVRRSSAVLEIHQEv* 0

>NEUR1_macEug
0  1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSy 1
2 GTWLKRHHAYICLVIIWAYATFWATMPLAGLGNYAPEPFGTSCTLDWWLAQASVTGQTFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQSSHVLEMKLTK 0
0  2
1 RHTVSTIRKSSSVSETYQEV* 0

>NEUR1_sarHar Sarcophilus harrisii (tasmanian_devil) 4 of 6 exons
0 1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAYICLVIIWAYATFWATMPLAGLGNYAPEPFGTSCTLDWWLAQASVTGQTFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKAAKKESLRDYR 2
1 * 0

>NEUR1_ornAna
0 MTNYSAPQLGDYLPHYLREGDPFVSKLSWEADLVAGVYLVII 1
2 GVLSTLGNGYVIYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIVSCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAYICLAIIWAYASFWATMPLVGLGNYAPEPFGTSCTLDWWLAQASVAGQAFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPIQFSVVPTLLAKSAAMYNPIIYQVIDCRISCCRLGGPKTGKKESLKNSR 2
1 SHSMSTIRKPSAVSGPHQEV* 0

>NEUR1_galGal
0 MASDCNSSSQEEYLPHYMQQEDPFASKLSREADIIAGFYLTVI 1
2 GILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIISFFSHRWIFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLAY 1
2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCRSGGPKTLQKKSSLKESR 2
1 MYTISSHRDSAALSGTQLEV* 0

>NEUR2_galGal Gallus gallus GenBank 5'UTR mistranslated as coding -B4GALT6 -NEUR2_galGal -KIAA1012
0 MDPSFANSTFQSKITEAADIVVGTCYMVF 1
2 GICSLCGNSILLYISYKKKHLLKPAEYFIINLAISDLAMTLTLYPLAVTSSLSHR 2
1 WLYGKHICLFYAFCGLFFGICSLSTLTLLSVVCCLKICFPAY 1
2 GNRFRRKHGQILIACAWTYAAIFACSPLAHWGEYGEEPYGTACCIDWQSTNVDVMSMSYTVVLFVLCFILPCGVIVTSYSLILVTVKESRKAVEQHVSGPTRINNVQTITAK 0
0 LSIAVCIGFFAAWSPYAIIAMWAAFGSIDKIPPLAFAIPAVFAKSSTLYNPIIHLLLKPNFRSNIAKDFTVIQQLCVRCCFCVKELQTYRSTFNTGLRTFKGKNESSCNALPIMEG
CSYFPSEKGSHTFECFKSYPNCFQERLSTMGCHLQDCESLENDLQVEVTQGSRNSMKVVEQEEKSTELDNLEITLEAVPVSCTFTDL* 0

>NEUR3_galGal Gallus gallus cOpn5L2 mRMA for Opsin 5-like 2 AB368183 chr3 XM_420056 CN231992 testis exon 2^3 rel NEUR1/2
0 MEEQYISKLHPVVDYGAGVFLLII 1
2 AILTILGNSAVLATAVKRSSLLKSPELLTVNLAVADIGMAISMYPLAIASAWNHAWLGGDASCIYYALMGFLFGVCSMMTLCAMAVIRFLVTNSSKSN 1
2 SNKISKNTVHILITFIWLYSLLWAILPLVGWGYYGPEPFGISCTIAWSKFHSSSNGFSFILSMFLLCTVLPALTIVACYLGIAWKVHKAYQEIQNINRIPHAAKLEKKLTL 0
0 MAVLISVGFLSAWTPYAAASFWSIFNSSDSLQPIVTLLPCLFAKSSTAYNPFIYYIFSKTFRHEIKQLQCCWGWRVHFFSADNSAENSVSMMWSGRDNIRLSPTAKVESQGAARH*

>NEUR4_ornAna Ornithorhynchus anatinus (platypus) XM_001508128  
0 MSLSHSLQVPWRNNLTFLNKEAQVSEQGETIIGIYLLAL 1
2 GWMSWFGNSMVIFILHRQRGILNPTDYLTFNLAVSDASVSVFGYSRGIIEIFNVFRDDGFLITSIWTCQ 0
0 VDGFLTLLFGLASINTLAMISVTRYIKGCHPHR 1
2 GHFINTANISVALILIWVSALFWSAGPVLGWGSYT 1
2 DRMYGTCEIDWAEANFSSICKSYIISIFFCCFFLPVSIMFFSYVSIIKMVKSSHTLAGADDPTDRQRRLDRDVTR 0
0 VSVVICTAFIVAWSPYAVISMWSAFGHSVPNLTSVLASLFAKSASFYNPIIYFGMNSKFRKDILVLLPCAKESKEPVKLKKFKNLRQKQGFTLQKPEKAHVLQVPDSGPMSLINTPPLGNRNSFDLACDNSDFECVRL* 0

Melanopsin (3+ marsupials)

Here Sarcophilus can be expected to have the main melanopsin but not the paralog MEL2 which terminates in sauropods

>MEL1_homSap Homo sapiens (human) Gq -GRID1 -WAPAL +LDB3 +BMPR1A 483 aa NM_033282 melanopsin OPN4   
0 MNPPSGPRVPPSPTQEPSCMATPAPPSWWDSSQSSISSLGRLPSISPT 0 
0 APGTWAAAWVPLPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGNGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTLTSHTSNLSWISIRRRQESLGSESEV 0
0 GWTHMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGHEAETPGK 0
0 TKGLIPSQDPRM* 0

>MEL1_proCap
0 MNPPWGPRVPSRPAQEPSCMSTPASAGRWDSSQATASSLAELPPSSPT 0
0 EARTQTADWVPFPTVDVPDYAHYTLGTVILLVGLTGVLGNLMVIYIFFR 2
1 SRGLRTPANMFIINLAISDFLMSLTQAPVFFASSLYKRWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRTALVLLGTWLYALAWSLPPFFGW 1
2 SAYVPDGLLTSCSWDYKSFMPSARTYTMLLCCFVFFLPLLVIIYCYVFIFKAIRETGR 2
1 ALQTFGACEGASETPRQWQRLQSEWKMAKIALLAILLYVLSWAPYSTVALVGFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAQHLPCLGVLLGVSDQHTRPYTSYRSTHHSTLSSQASDISWISGRRRQASLGSESEV 0
0 GWTDTEAAAAWEGAQQVSGRASCSQVLESMEANTPPRPQGWGPETPRK 0
0 VKGLPLLDPRA* 0

>MEL1_smiCra Sminthopsis crassicaudata (dunnart) DQ383281
0 MNPSPMLRHLSCPAQDSNCTKIMASISEWNNTEVDAYHLVDLPPITPT 0
0 AVVLPPYSQKVFPTADVPDYAHYTIGATILVVGFTGVLGNLLVIYTFCR 2
1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFASSLYERWIFGEK 2
1 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYTTFTPSVRAYTILLFCFVFFIPLTVIIYCYIFIFRAIKDTNK 2
1 AVQNIGSSEHTPSLRHFQRMKNEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2
1 YSHVLTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2
1 MAIAQNFPCLRAVLGIRHPRTQSFSSYRFTHRSTTASQASDISWQSRGRRQLSLGSESEA 0
0 GWNNIETGLTLRSLEGSCGMDEETMDTRELSASTKAKGQSWETLAKTLEE 0
0 MDDLSLLEAGTLLSSLDLQI* 0

>MEL1_sarHar Sarcophilus harrisii (tasmanian_devil)96% identity smiCra last exon missing FKUJDAX01C1KMN needed
0 MNPSPMLRHLSCSAQDTNCTKIMASISEWNNTEVDAYHLVDLPPITPT 0
0 AVVLPPYSQNVFPTADVPDYAHYTIGATILVVGFTGVLGNLLVIYTFCR 2
1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFASSLYKRWIFGEK 2
1 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYTTFTPSVRAYTILLFCFVFFIPLIVIIYCYIFIFRAIKDTNK 2
1 AVQNIGSRASTPSPRHFQRMKNEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2
1 YSHVLTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2
1 MAIAQNFPCLRAVLGIRHPRTQSFSSYRFTHRSTTASQASDISWQSRGRRQLSLGSESEA 0
0 GWNNIEAGIEGLTLRSLEGYCGMDEETMETREPSASAKAKGQ    0 
0 * 0

>MEL1_macEug Macropus eugenii frag
0 AVVLPPHSRNIFPTADVPDHAHYTVGAIILVVGFTGVLGNLLVIYTFCR 2
1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFANSLYKRWIFGEK 2
2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGVVSKKKTGLILLGVWLYSLAWSLPPFFGW 1
2 AYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFKAIQDTNK 2
1 ALQNIRSSESTASPRHFQRMKSEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2
1 SHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2

>MEL1_monDom Monodelphis domestica (opossum) Gq -GRID1 -WAPAL +LDB3 +BMPR1A
0 MNPSPMLRGLSCPAQDTNCTKIMASMSEWNNTEEDAYHLVDLPSIAPT 0
0 AVVLPPSSQNIFPTVDVPDHAHYTIGAIILAVGITGMLGNFLVIYTFCR 2
1 SHSLRTPANMFIINLAISDFFMSFTQAPVFFASSMYKRWIFGEK 1
2 ACEFYAFCGALFGITSMITLMAIALDRYFVITRPLASIGVISKKKTGFILLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFRAIQDTNK 2
1 AVHSIGSGESTASPRHCQRMKNEWKMAKIALVVILLYVLSWAPYSTVALVAFAG 2
1 YSHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2
1 MAIAQNFPCLRALLCVRHPRTRSFSSYRFTRRSTMTSQASDISWLPRGRRQLSLGSESEI 0
0 GWNNMEAGTTSLTSRNQQGSCRMDQETMETRELAAIAKAKGRSWETLEK 0
0 TLEEMDDSSLLEVSVDMEQ* 0

>MEL1_ornAna Ornithorhynchus anatinus (platypus) fragment
0 0
0   FPTADVPDHAHYTIGATILAVGFTGVLGNLLVIYTFCR 2
1 SRSLRTPANMFIINLSISDFFMSLTQAPVFFASSLHKRWIFGEK 1
2 GCQLYAFCGALFGITSMITLTVIALDRYFVITRPLASIGVISKKRALLILTGVWFYSLAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYMTFTPPVRAYTMLLFCFVFFIPLIMIIYCYFFIFRAIRGTNK 2
1 AVETIGSDDCRGSQRQCQRMKNEWKTAKIALMVILLYVISWCPYSVVALVAFAG
1 YSHLLTPYMNSVPAVIAKSSAIHNPIIYAITHPKYR 2
1 MAITKYIPCLGPLLRVSRQDSRSSSHYASSRRSTVTSQSLDGSWLPGRRRPLSSASDSES 0
0 0
0 * 0

>MEL1_anoCar Anolis carolinensis diverged frag
0 0
0 ERTMFNLPDPFPTVDVPTHAHYTIGAVILVVGITGTLGNLLVIYVFFR 2
1 IRGLRTPANMFVINLAVSDFL 1
2 GCELYAFCGALFGIASMITLTVIALDRYFVITRPLASIGAMSTKKALLILSGVWLYSLAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYITFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFIAIKNSNR 2
1 AVQRTNSDNSKEGQKLYQKLKNEWKMAKVALIVILLVISWSPYSVVALVAFAG 2
1 YSHLLTPYMNSVPAVIAKASVIHNPIIYAIVHPKYR 2
1 MAIAKFLPCLGSLLRVPRKDSSYPSTRRPTVTSQSSDINGVPRGHRRLSSVSDSES 0
0 DWTDTEADISSQNSRVASGSISYRIYEDTTETIKVKSKMRSHDSGIFER 0
0 0
0 TGEDLNAFGWRREESYSGPSTSSQIPSIIVTFSNVQRTDLPLESSSGALCSRNSSYSWEKDSNS* 0

>MEL1_galGal Gallus gallus (chicken) Gq short exon 1 -GRID1 -WAPAL +LDB3 +BMPR1A 529 aa 16856781 AY88294 melanopsin OPN4m   
0 MDLPPRAPT 0
0 KMTVKDVRGAFPTVDVPDHAHYTIGTVILIVGITGTLGNFLVIYAFCR 2
1 SRTLQKPANIFIINLAVSDFLMSITQSPVFFTNSLHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVRVMSKKKALIILVGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFEAIKKANK 2
1 SVQTFGCKHGNRELQKQYHRMKNEWKLAKIALIVILLYVISWSPYSVVALVAFAG 2
1 YSHVLTPFMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 TAIATYVPCLGFLLRVSPKESRSFSSYPSSRRTTITSQSSETSGLQKGKRRLSSISDSES 0
0 GCTDTETDITSMISRPASSQVSYEMGEDTTQTSDLGGKPKVKSHDSGIFRK 0
0 TVVDADEIPMVEINDTEHSATSTCKTSEKCNVEEIQ 0
0 RSESLSGIGLREGESRHRTSASQIPSIIITYSNVQGVELHSGYSAGFLHPKNKSHKQNKSSNS* 0

>MEL2_galGal Gallus gallus (chicken) Gq 0.0.1.2.2.1.1.1.0.0 indel +GRID2 +SMARCAD1 -PGDS -SEC24B +COL25A1 544 aa 000 nm 17977531 NM_204625 full
0 MGTQPHSVTKSEIPDHVLYTVGTCVLVIGSIGIIGNLLVLYAFYS 2
1 NKKLRTPQNFFIMNLAVSDFLMSASQAPICFVNSLHREWILGDI 1
2 GCDLYAFCGALFGITSMMTLLAISVDRYLVITKPLRSIQWTSKKRTIQIIAAVWLYSLGW 1
2 SVAPLLGWSSYVPEGLMISCTWDYVTYSPANRSYTMILCCCVFFIPLIIILHCYLFMFLAIRSTGR 2
1 DVQKLGSCSRKSFLSQSMKNEWKLAKIAFVVIIVYVLSWSPYACVTLIAWAG 2
1 RGNTLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2
1 KTIHNAVPCLRFLIRISKNDLLRGSINESSFRTSLSSHQSLAGRTKNTCVSSVSTGEA 0
0 NWSDVELDTVEPAHEKLQPRRSHSFSSSLRQKRDLLPDSYSCSEETEEK 0
0 VSLSSSYLEKVLGRSAFPSSPVALVTSSLRAASLPVGLNSSSASRGAGSDISQMKTEESHNNGGLDSIVSNTVPQIIIIPTSETNLFQEEPEEEETELFHFHDKKNNLLDLEGLSSSTEFLEAVEKFLS* 0

PRNP (3+ marsupials)

The Sarcophilus repeat region is of considerable interest -- the high GC content of this region makes it difficult to sequence and so provides a test of the 454 technology and Newbler assembler. This region consists in placentals a five octapeptide repeat, in marsupials and platypus a five nona- or decapeptide residue repeat that may resolve fine details of the marsupial phylogenetic tree, which in birds, lizards, turtles, frogs and fish is a hexapeptide repeat with trimeric internal substructure. Even though the single exon gene is clearly orthologous in all these species, the repeat regions within it are not directly comparable because they have expanded and contracted through replication slippage, plus experienced the odd repeat length change in marsupials and another in placentals.

The Sarcophilus prion gene has very high coverage that overcomes the occasional problem with frameshifts and allows the gene to be accurately tiled. However familiarity with the gene and reliable fiducial sequences are key to rapid assembly of the full length gene. No sequencing difficulties were observed in the high GC repeat region. The gene is very normal and has no indications whatsoever of abnormal numbers of repeats (4) or prion disease disposition.

Dasypus         MVRSRVGCWLLLLFVATWSELGLC KK.RPKPGGGWNTGG  SRYPGQ GSPGG NRYP     PQGGG  WGQ PHGGG  WGQ PHGGG  WGQ PHGGG  WGQ PHGGG  WGQ  GGAHGQ                
Trichosurus     MGKIQLGYWILVLFIVTWSDLGLC KKPKPRPGGGWNSGGS NRYPGQPGSPGG NRYPGWGH PQGGGTNWGQ PHPGGSNWGQ PHPGGSSWGQ PH GGSNWGQ             GG YN  
Sarcophilus     MGKIRLGYWILALFIVTWSDLGLC KKPKPRPGGGWNSGGS NRYPGQPGSAGG NRYPGWGH PQGGGTNWGQ PHPGGSSWGQ PHAGGSNWGQ PH.GGSNWGQ            SGSSYNQ
Monodelphis     MGKIHLGYWFLALFIMTWSDLTLC KKPKPRPGGGWNSGG  NRYPGQ    SG     GWGH PQGGGTNWGQ PHAGGSNWGQ PRPGGSNWGQ PHPGGSNWGQ PHPGGSNWGQ AGSSYNQ 
Macropus        MAKIQLGYWILALFIVTWSELGLC KKPKTRPGGGWNSGGS NRYPGQPGSPGG NRYPGWGH PQGGGTNWGQ PHPGGSSWGQ PHAGGSNWGQ PH.GGSNWGQ            GGGSYG
Ornithorhynchus ------------------------ -------GGGWNSG   NRYPGQPANPG      GWGH PQGGGASWGH PQGGGASWGH PQGGGSNWGH PQGGGASWGH PQ          GGGYS  

Dasypus         WNKPSKPKTNM KHVAGAAAAGAVVG LGGYLVGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRSVEQYSSEKNFVHD CV                         MERVVEQMCITQYQ 
Trichosurus     KWKPDKPKTNL KHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFGNEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN
Sarcophilus     KWKPDKPKTNM KHMAGAAAAGAVLGSLGGYVLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN
Monodelphis     KWKPDKPKTNM KHVAGAAAAGAVVGGLGGYMLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYNNQNNFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN
Macropus        KWKPDKPKTNL KHVAGAAAAGAVVGGLGGYMLGSAMSRPVMHFGNEYEDRYYRENQYRYPNQVMYRPIDQYGSQNSFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN
Ornithorhynchus KYKPDKPKTGM KHVAGAAAAGAVVGGLGGYMIGSAMSRPPMHFGNEFEDRYYRENQNRYPNQVYYRPVDHFCSQDGFVRD CVNITVTQHTVTTT.EGKNLNETDVKIMTRVLEQMC 

The signal region of Sarcophilus PRNP is expected to show the same length as the other 3 known marsupial sequences, which is confirmed by the sequence. Placentals exhibit a one residue deletion relative to this ancestral length.

MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Homo sapiens
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Pan troglodytes
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Gorilla gorilla
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Pongo pygmaeus
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Nomascus leucogenys
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Hylobates lar
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Symphalangus syndactylus
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca arctoides
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca fascicularis
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca fuscata
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca mulatta
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca nemestrina
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Papio hamadryas
MA--NLGCWMLFLFVATWSDLGLCKK--RPKPG Callithrix jacchus
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Cebus apella
MA--NLGCWMLVVFVATWSDLGLCKK--RPKPG Cercopithecus aethiops
MA--NLGCWMLVVFVATWSDLGLCKK--RPKPG Cercopithecus dianae
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Colobus guereza
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Presbytis francoisi
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Saimiri sciureus
MA--KLGYWLLVLFVATWSDVGLCKK--RPKPG Tarsius syrichta
MA--NLGCWMLVVFVATWSDVGLCKK--RPKPG Microcebus murinus
MA--RLGCWMLVLFVATWSDIGLCKK--RPKPG Otolemur garnettii
ME--NLGCWMLILFVATWSDIGLCKK--RPKPG Cynocephalus variegatus
MA--QLGCWLMVLFVATWSDVGLCKK--RPKPG Tupaia belangeri
MA--NLGYWLLALFVTMWTDVGLCKK--RPKPG Mus musculus
MA--NLGYWLLALFVTTCTDVGLCKK--RPKPG Rattus norvegicus
MA--NAGCWLLVLFVATWSDTGLCKK--RPKPG Cavia porcellus
MA--NLGCWLLVLFVATWSDLGLCKK--RTKPG Dipodomys ordii
MV--NPGCWLLVLFVATLSDVGLCKK--RPKPG Spermophilus tridecemlineatus
MA--HLGYWMLLLFVATWSDVGLCKK--RPKPG Oryctolagus cuniculus
MA--HLSYWLLVLFVAAWSDVGLCKK--RPKPG Ochotona princeps
MVKSHIGSWILVLFVAMWSDVGLCKK--RPKPG Bos taurus
MVKSHIGGWILVLFVAAWSDIGLCKK--RPKPG Sus scrofa
MVKSHMGSWILVLFVVTWSDMGLCKK--RPKPG Vicugna vicugna
MVKSHVGGWILVLFVATWSDVGLCKK--RPKPG Equus caballus
MVRSHVGGWILVLFVATWSDVGLCKK--RPKPG Diceros bicornis
MVKSLVGGWILLLFVATWSDVGLCKK--RPKPG Myotis lucifugus
MVKNYIGGWILVLFVATWSDVGLCKK--RPKPG Pteropus vampyrus
MVKSHIANWILVLFVATWSDMGFCKK--RPKPG Tursiops truncatus
MVKSHIGGWILLLFVATWSDVGLCKK--RPKPG Canis lupus familiaris
MVKSHIGSWILVLFVAMWSDVGLCKK--RPKPG Felis catus
MVKSHIGSWLLVLFVATWSDIGFCKK--RPKPG Mustela putorius
MVKSHIGSWLLVLFVATWSDIGFCKK--RPKPG Mustela vison
MVKSHIGSWILVLFVAMWSDVGLCKK--RPKPG Ailuropoda melanoleuca
MVKNHVGCWLLVLFVATWSEVGLCKK--RPKPG Erinaceus europaeus
MVTGHLGCWLLVLFMATWSDVGLCKK--RPKPG Sorex araneus
MVKSHLGCWIMVLFVATWSEVGLCKK--RPKPG Cyclopes didactylus
MVRSRVGCWLLLLFVATWSELGLCKK--RPKPG Dasypus novemcinctus
MVKGTVSCWLLVLVVAACSDMGLCKK--RPKPG Echinops telfairi
MVKSSLGCWILVLFVATWSDMGLCKK--RPKPG Loxodonta africana
MVKSSLGCWMLVLFVATWSDVGLCKK--RPKPG Procavia capensis
MAKIQLGYWILALFIVTWSELGLCKKP-KTRPG Macropus eugenii
MGKIHLGYWFLALFIMTWSDLTLCKKP-KPRPG Monodelphis domestica
MGKIRLGYWILALFIVTWSDLGLCKKP-KPRPG Sacophilus harrisii
MGKIQLGYWILVLFIVTWSDLGLCKKP-KPRPG Trichosurus vulpecular
MARLLTTCCLLALLLAACTDVALSKKG-KGKPS Gallus gallus
MAKLPGTSCLLLLLLLLGADLASCKKG-KGKPG Taeniopygia guttata
MARLLTTCCLLALLLAACTDVALSKKG-KGKPG Meleagris gallopavo
MGKHQMTCWLAIFLLLIQANVSLAKK--KPKPS Anolis carolinensis
MRRFLVTCWIAVFLILLQTDVSLSKKG-KNKPG Gekko gekkko
MGRYRLTCWIVVLLVVMWSDVSFSKKG-KGKGG Trachemys scripta (turtle)
MGRHLISCWIIVLFVAMWSDVSLAKKG-KGKTG Pelodiscus sinensis (turtle)
MPQSLWTCLVLISLICTLTVSSKKSGGGKSKTG Xenopus laevis
MLRSLWTSLVLISLVCALTVSSKKSGSGKSKTG Xenopus topicalis

>PRNP_sacHar Sarcophilus harrisii (tasmanian_devil) single exon gene YVLG like Dasypus
MGKIRLGYWILALFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGSAGGNRYPGWGHPQGGGTNWGQPHPGGSSWGQPHAGGSNWGQPHGGSNWGQ
SGSSYNQKWKPDKPKTNMKHMAGAAAAGAVLGGVGGYVLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTTTT
KGENFTETDIKIMERVVEQMCITQYQNEYRAAQYSYNMAFFSAPPVTLLLLGFLIFLIVS*

>PRNP_mdo Monodelphis domestica opossum, from frameshifted genomic
MGKIHLGYWFLALFIMTWSDLTLCKKPKPRPGGGWNSGGNRYPGQSGGWGHPQGGGTNWGQPHAGGSNWGQPRPGGSNWGQPHPGGSNWGQPHPGGSNWG
QAGSSYNQKWKPDKPKTNMKHVAGAAAAGAVVGGLGGYMLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYNNQNNFVHDCVNITVKQHTTT
TTTKGENFTETDIKIMERVVEQMCITQYQNEYRSAYSVAFFSAPPVTLLLLSFLIFLIVS*

>PRNP_tvu Trichosurus vulpecular brushtail opossum
MGKIQLGYWILVLFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGSPGGNRYPGWGHPQGGGTNWGQPHPGGSNWGQPHPGGSSWGQPHGGSNWGQGGY
NKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFGNEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTTTTKGENFTETDIKIMERVVEQM
CITQYQAEYEAAAQRAYNMAFFSAPPVTLLFLSFLIFLIVS*

>PRNP_meu Macropus eugenii (tammar wallaby)
MAKIQLGYWILALFIVTWSELGLCKKPKTRPGGGWNSGGSNRYPGQPGSPGGNRYPGWGHPQGGGTNWGQPHPGGSSWGQPHAGGSNWGQPHGGSNWGQ
GGGSYGKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVMHFGNEYEDRYYRENQYRYPNQVMYRPIDQYGSQNSFVHDCVNITVKQHTTTTTT
KGENFTETDIKIMERVVEQMCITQYQNEYQAAQRYYNMAFFSAPPVTLLLLSFLIFLIVS*
 
>PRNP_oan  Ornithorhynchus anatinus platypus fragment
PHWGKSPVHHWIIDICVVHLERRCRGHLHPNPCPGGRCVQQQPNRYPGQPATPGGWGHPQGGGASWGHPQGGGSNWGHPQGGGASWGHPQGGGYSKYKPDKPKTG
MKHVAGAAAAGAVVGGLGGYMIGSAMSRPPMHFGNEFEDRYYRENQNRYSNQVYYRPVDQYGSQDGFVRDCVNITVTQHTVTTTEGKNLNETDVKIMTRVLEQMCVNLY

PRND (2+ marsupials)

Sarcophilus sequence for this intronless gene is a welcome addition to a limited existing set of early-diverging mammalian orthologs. With more data, the relative rates of divergence of PRND from its parental paralog PRNP could be compared in marsupial and placentals. It appears from the mere 75% identity between tasmanian devil and wallaby that doppels are diverging quite rapidly both from PRNP and from each other in the marsupial lineage, indicating some selectional pressure but not a hugely important function (that is, many residue positions have an increased reduced alphabet).

>PRND_hsa Homo sapiens (human) full
MRKHLSWWWLATVCMLLFSHLSAVQTRGIKHRIKWNRKALPSTAQITEAQVAENRPGAFIKQGRKLDIDFGAEGNRYYEANYWQFPDGIHYNGCSEANVTKEAFVTGCINATQAANQGEFQKPDNKLHQQVLWRLVQELCSLKHCEFWLERGAGLRVTMHQPVLLCLLALIWLTVK*

>PRND_dno Dasypus novemcinctus
MRKHLGGWRLAIVCVLLSGHLSMVKARGIKHRIKWNRKAAPGAAQVTEARVAEQRPGAFVRQGRRLDIDFGAEGNRYYEANYWQLPDGILYDGCAEANVTKEALVAGCVNATQLANQAELAHEGQDTLHRRVLGRLIRELCALKRCKFWPDRAAGPRLVRGAPVFGGLLLLIWLLVR*

>PRND_laf Loxodonta africana African elephant Afrotheria 176 aa revised/corrected
MRKHLGAWWLAIAFVLLLSHLSMVTARGIKHRIKWNRKALPNTGHVTAAQVTETRPGAFIRHGRKLDIDFGAEGNRYYEANYWQFPDGIHYDGCSEANVTKEMFVTSCINTTQAANQEEFSRKQDNKVYQRILWRLIRELCSVKHCDFWLDRGGGLRVSLDQPVMLCLLVFIWFMVK*  

>PRND_sacHar Sarcophilus harrisii (tasmanian_devil) single exon gene 77% macEug
MRTPLETWWIAIFFTLLFSDLSLVKAKGIRQRNKSNRKSLQTNRANPTREQPSKILQGTFIRKGRKLSINFGEEGNSYYEAHYKLFPDEIHYVGCAESSVTKDVFISNCVNVTHTANKLEPPEERNSSAIYSRVLEQLIKELCALKYCEFGMQIGAGFRLSLDQSMMVYLMILAFFIVK*

>PRND_mdo Monodelphis domestica doppel genomic revised +rassf2 -prnd -prnp
MRRHLGICWIAIFFALLFSDLSLVKAKTTRQRNKSNRKGLQTNRTNPTTVQPSEKLQGTFIRNGRKLVIDFGEEGNSYYATHYSLFPDEIHYAGCAESNVTKEVFISNCVNATRVINKLEPLEEQNISDIYSRILEQLIKELCALNYCEFRTGKGTGLRLSLDQYVMVYLVILTCLIVK*

>PRND_meu Macropus eugenii wallaby 
MRRHLGTWWTAIFFALLFSDLSLVKAKGTRQRNKSNRKSLQTNRVNPTTAQPSEILQGAFIRQGRKLSIDFGEEGNSYYETHYQLFPDEIHYVGCTESNVTKDIFISNCMNATHAVNNLETLEEKNASDIHSRVLEQLIKELCALKYCELETETGAGLKLSLDQSVMVYLVILTCLIVK*

>PRND_oan Ornithorhynchus anatinus  platypus 42% to opposum 187 aa 4 cys in register
MMTVRRRRRSGGARWLLVFLVLLSGDLSSLQARGPRPRNKAGRKPPPSNAGPDSPAPRPPAGARGTFIRRGGRLSVDFGPEGNGYYQANYPLLPDAIVYPDCPTANGTREAFFGDCVNATHEANRGELTAGGNASDVHVRVLLRLVEELCALRDCGPALPTGPAPRPGPPGPPAALALLTLVLLGAQ*

>PRND_aca Anolis carolinensis weak but real! scaffold_1221:78,884-117,121 syntenic, oriented like PRNP but no larger
MMQRPLVVAILLTALWSEVCLCRRVSGSANRRNKKTSTTTSAPKLQSSTTATTFQGNLCRGGQMIDNMDLEPNDKVYYKANLKIFPDGLYYPNCSLLLQPNTTKEELVGECVNFTIASNKLNLSKGKDLSNTKERVMWVLIHHLCANESCGQPCPLLQNSGNLHYIGQVLTVFVGLIGCSFLSAK*