Opsin evolution: Melanopsin gene loss: Difference between revisions

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'''See also:''' [[Opsin_evolution|Curated Sequences]] | [[Opsin_evolution:_LWS_PhyloSNPs|LWS]] | [[Opsin_evolution:_Encephalopsin_gene_loss|Encephalopsins]] | [[Opsin_evolution:_Neuropsin_phyloSNPs|Neuropsins]] | [[Opsin_evolution:_Peropsin_phyloSNPs|Peropsins]] | [[Opsin_evolution:_RGR_phyloSNPs|RGR phyloSNPs]] | [[Opsin_evolution:_update_blog|Update Blog]]
== Introduction to Melanopsin Evolution ==
== Introduction to Melanopsin Evolution ==


(more shortly)
Melanopsins form one of the five basic classes of bilateran opsins. Frog dermatocytes provided the index sequence for which the gene family is named, though melanopsins are more typically expressed elsewhere. The opsin research community has been slow to recognize that ecdysozoan imaging opsins do not form a separate opsin class but in fact are orthologous to deuterostomal melanopsins. Here by definition orthologous means vertically descended from a single gene in the last common ancestor (which allows for multiple independent lineage-specific expansions and divergences).
 
The unity of melanopsins follows from agreement of 5 largely independent methods: alignment of all opsins into trees, best-blast clustering, diagnostic conserved residues specific to opsin class, informative indels, and synapomorphic intron positions and phases. It is especially hard to understand, given over a thousand possible intron locations in a protein of opsin length, how introns of insect and octopus melanopsins could otherwise precisely agree (after restricting to ancestral) with those of vertebrate melanopsins.


=== Melanopsin MEL1 retention with exon loss in mammals ===  
=== Melanopsin MEL1 retention with exon loss in mammals ===  


Melanopsin is an ancient rhabodomeric Gq opsin that persists all examined extant deuterostomes (which otherwise use ciliary opsins and the peropsin/neuropsin/RGR opsins). The melanopsins in earlier diverging clades are remarkable for their greatly extended C-terminal extension whose location is presumably cytoplasmic and whose function is quite unknown. This extenstion has undergone rapid change in post-frog species such as birds and lizards resulting in novel exons, diminished distal homology -- and in mammals, complete loss of terminal exons and any functionality that they provided.  
Melanopsin is an ancient rhabodomeric Gq opsin that persists all examined extant deuterostomes (which otherwise use ciliary opsins and the peropsin/neuropsin/RGR opsins). The melanopsins in earlier diverging clades are remarkable for their greatly extended C-terminal extension whose location is presumably cytoplasmic and whose function is quite unknown. This extension has undergone rapid change in post-frog species such as birds and lizards resulting in novel exons, diminished distal homology -- and in mammals, complete loss of terminal exons and any functionality that they provided.  


Less drastic length alterations occur at the amino terminus as well and indels are far more common than in other opsin families. This raises questions about what lies behind these dramatic shifts. The recent determination of the structure of squid melanopsin -- with a big role for its cytoplasmic tail -- won't be replicated in non-cephalopod melanopsins because conservation is observed only to a proline 8 of  127 residue past the FR motif. Even central conservation rapidly drops below 45% even within other lophotrochozoans. Consequently the 25 angstrom cytoplasmic knob of squid melanopsin has no value to annotation transfer and probably has very little to do with Galpha signaling specificity.
Less drastic length alterations occur at the amino terminus as well and indels are far more common than in other opsin families. This raises questions about what lies behind these dramatic shifts. The recent determination of the structure of squid melanopsin -- with a big role for its cytoplasmic tail -- won't see annotation transfer to non-cephalopod melanopsins because conservation is observed only to a proline 8 of  127 residues past the FR motif. Even central conservation rapidly drops below 45% even within other lophotrochozoans. Consequently the 25 angstrom cytoplasmic knob of squid melanopsin has no value to annotation transfer and probably has very little to do with Galpha signaling specificity.


The comparative genomics of a small deletion in exon 4 of bat melanopsin is shown below; its functional significance for the DRY second cytoplasmic loop (much less photoreception) is unknown. The residue otherwise is quite well conserved within mammals and restricted to A, S, or T in all vertebrate melanopsins.
The comparative genomics of a small deletion in exon 4 of bat melanopsin is shown below; its functional significance for the DRY second cytoplasmic loop (much less photoreception) is unknown. The residue otherwise is quite well conserved within mammals and restricted to A, S, or T in all vertebrate melanopsins.
Line 31: Line 35:
  2 GCEFYAFCGALFGITSMITLMAIALDRYLVITHPLATIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_felCat Felis catus (cat)
  2 GCEFYAFCGALFGITSMITLMAIALDRYLVITHPLATIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_felCat Felis catus (cat)
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGVVSKRWAALVLLGIWLYALAWSLPPFFGW 1  MEL1_equCab Equus caballus (horse)
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGVVSKRWAALVLLGIWLYALAWSLPPFFGW 1  MEL1_equCab Equus caballus (horse)
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPL<font color="blue">A-I</font>GVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_myoLuc Myotis lucifugus (microbat)  7/7 traces
  2 GCEFYAFCGALFGITSMITLTAIAL<font color="red">DRYLVITRPLA-IGVVSKRR</font>AALVLLGVWLYALAWSLPPFFGW 1  MEL1_myoLuc Myotis lucifugus (microbat)  7/7 traces
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLAAIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_pteVam Pteropus vampyrus (macrobat) 3/3 traces
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLAAIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_pteVam Pteropus vampyrus (macrobat) 3/3 traces
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_bosTau Bos taurus (cow)
  2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_bosTau Bos taurus (cow)
Line 54: Line 58:
=== Melanopsin MEL2 gene loss in mammals ===  
=== Melanopsin MEL2 gene loss in mammals ===  


A paralogous [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=16856781 second] melanopsin in nonmammalian vertebrates was found quite recently by Bellingham et al. It is still unclear whether this gene is present in chondrichthyes or lamprey because of the limited coverage of current assemblies. This evidently arose prior to divergence of telost fish by a segmental duplication because some evidence of flanking synteny survives. It cannot have arisen by the whole genome duplication in fish because that would not have descended to frog, lizards, and birds. Instead all whole genomic duplication copies of fish melanopsins were lost. No sign of this second gene can be found in platypus, marsupials, or placental mammals -- yet another longstanding functional opsin gene lost in this clade.
A paralogous [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=16856781 second] melanopsin in non-mammalian vertebrates was found quite recently by Bellingham et al. It is still unclear whether this gene is present in chondrichthyes or lamprey because of the limited coverage of current assemblies. This evidently arose prior to divergence of teleost fish by a segmental duplication because some evidence of flanking synteny survives. It cannot have arisen by the whole genome duplication in fish because that would not have descended to frog, lizards, and birds. Instead all whole genomic duplication copies of fish melanopsins were lost. No sign of this second gene can be found in platypus, marsupials, or placental mammals -- yet another longstanding functional opsin gene lost in the mammalian crown group.


<pre>Transmembrane, DRY loop, and Schiff base regions of human MEL1 are very similar to lizard MEL2:
<pre>Transmembrane, DRY loop, and Schiff base regions of human MEL1 are very similar to lizard MEL2:
Line 705: Line 709:
1 NHRRTASTLFVTIVVFLFAWFPYCIVSLWVLIGDANSISKLSTTIPSLFAKSSVIYNPLIYVVLNSKFRKALIQTLSFLKCLSKHELSESS* 0
1 NHRRTASTLFVTIVVFLFAWFPYCIVSLWVLIGDANSISKLSTTIPSLFAKSSVIYNPLIYVVLNSKFRKALIQTLSFLKCLSKHELSESS* 0
</pre>
</pre>
'''See also:''' [[Opsin_evolution|Curated Sequences]] | [[Opsin_evolution:_LWS_PhyloSNPs|LWS]] | [[Opsin_evolution:_Encephalopsin_gene_loss|Encephalopsins]] | [[Opsin_evolution:_Neuropsin_phyloSNPs|Neuropsins]] | [[Opsin_evolution:_Peropsin_phyloSNPs|Peropsins]] | [[Opsin_evolution:_RGR_phyloSNPs|RGR phyloSNPs]] | [[Opsin_evolution:_update_blog|Update Blog]]
[[Category:Comparative Genomics]]
[[Category:Comparative Genomics]]

Latest revision as of 11:24, 23 March 2010

See also: Curated Sequences | LWS | Encephalopsins | Neuropsins | Peropsins | RGR phyloSNPs | Update Blog

Introduction to Melanopsin Evolution

Melanopsins form one of the five basic classes of bilateran opsins. Frog dermatocytes provided the index sequence for which the gene family is named, though melanopsins are more typically expressed elsewhere. The opsin research community has been slow to recognize that ecdysozoan imaging opsins do not form a separate opsin class but in fact are orthologous to deuterostomal melanopsins. Here by definition orthologous means vertically descended from a single gene in the last common ancestor (which allows for multiple independent lineage-specific expansions and divergences).

The unity of melanopsins follows from agreement of 5 largely independent methods: alignment of all opsins into trees, best-blast clustering, diagnostic conserved residues specific to opsin class, informative indels, and synapomorphic intron positions and phases. It is especially hard to understand, given over a thousand possible intron locations in a protein of opsin length, how introns of insect and octopus melanopsins could otherwise precisely agree (after restricting to ancestral) with those of vertebrate melanopsins.

Melanopsin MEL1 retention with exon loss in mammals

Melanopsin is an ancient rhabodomeric Gq opsin that persists all examined extant deuterostomes (which otherwise use ciliary opsins and the peropsin/neuropsin/RGR opsins). The melanopsins in earlier diverging clades are remarkable for their greatly extended C-terminal extension whose location is presumably cytoplasmic and whose function is quite unknown. This extension has undergone rapid change in post-frog species such as birds and lizards resulting in novel exons, diminished distal homology -- and in mammals, complete loss of terminal exons and any functionality that they provided.

Less drastic length alterations occur at the amino terminus as well and indels are far more common than in other opsin families. This raises questions about what lies behind these dramatic shifts. The recent determination of the structure of squid melanopsin -- with a big role for its cytoplasmic tail -- won't see annotation transfer to non-cephalopod melanopsins because conservation is observed only to a proline 8 of 127 residues past the FR motif. Even central conservation rapidly drops below 45% even within other lophotrochozoans. Consequently the 25 angstrom cytoplasmic knob of squid melanopsin has no value to annotation transfer and probably has very little to do with Galpha signaling specificity.

The comparative genomics of a small deletion in exon 4 of bat melanopsin is shown below; its functional significance for the DRY second cytoplasmic loop (much less photoreception) is unknown. The residue otherwise is quite well conserved within mammals and restricted to A, S, or T in all vertebrate melanopsins. 


Myotis indel in cytoplasmic loop between transmembrane segments 3 and 4
.....FYAFCGALFGISSMITLTAIA.......................FVLLGVWLYALAWSLPPFFGW
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1  MEL1_homSap Homo sapiens (human) melanopsin OPN4
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1  MEL1_panTro Pan troglodytes (chimp)
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATIGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1  MEL1_ponpyg Pongo pygmaeus (orang_sumatran)
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATIGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1  MEL1_macMul Macaca mulatta (rhesus)
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLTTVGVASKRRAALVLLGVWLYSLAWSLPPFFGW 1  MEL1_otoGar Otolemur garnettii (bushbaby)
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLASVGTASKRRAGLVLLGVWLYALAWSLPPFFGW 1  MEL1_micMar Microcebus murinus (mouse_lemur)
2 GCEFYAFCGAVFGITSMITLTAIAMDRYLVITRPLATIGRGSKRRTALVLLGVWLYALAWSLPPFFGW 1  MEL1_musMus Mus musculus (mouse)
2 GCKFYAFCGAVFGIVSMITLTAIAMDRYLVITRPLATIGMRSKRRTALVLLGVWLYALAWSLPPFFGW 1  MEL1_ratNor Rattus norvegicus (rat)
2 GCEFYAFCGAVLGITSMITLTAIALDRYLVITRPLATIGMGSKRRTALVLLGIWLYALAWSLPPFFGW 1  MEL1_phoSun Phodopus sungorus (hamster)
2 GCEFYAFCGAVFGISSMITLTAIALDRYLVITRPLATIGMASKKRAAFFLLGVWFYALAWSLPPFFGW 1  MEL1_speTri Spermophilus tridecemlineatus (squirrel)
2 GCEFYAFCGAVSGITSMTTLTAIALDRYLVITRPLATIGVASKRRTALVLLGVWLYALAWSLPPFFGW 1  MEL1_nanSpa Nannospalax ehrenbergi (mole-rat)
2 GCEFYAFCGALFGITSMITLTAITLDRYLVITRPLATIGVASKRQAALVLLGVWLYALAWSLPPFFGW 1  MEL1_cavPor Cavia porcellus (guinea_pig)
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLAAVGMVSKKRAGLVLLGVWLYALAWSLPPLFGW 1  MEL1_oryCun Oryctolagus cuniculus (rabbit)
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLAAVGMVSKRRTGLVLLGVWLYSLACSLPPLFGw 1  MEL1_ochPri Ochotona princeps (pika)
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITHPLAAVGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_canfam Canis familiaris (dog)
2 GCEFYAFCGALFGITSMITLMAIALDRYLVITHPLATIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_felCat Felis catus (cat)
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGVVSKRWAALVLLGIWLYALAWSLPPFFGW 1  MEL1_equCab Equus caballus (horse)
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLA-IGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_myoLuc Myotis lucifugus (microbat)  7/7 traces
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLAAIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_pteVam Pteropus vampyrus (macrobat) 3/3 traces
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_bosTau Bos taurus (cow)
2 GCEFYAFCGAVFGITSMITLTAIALDRYLVITRPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_turTru Tursiops truncatus (dolphin)
2 GCEFYAFCGAVFGITSMITLTAIALDRYLVITYPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_susScr Sus scrofa (pig) VPT
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRVALVLLGVWLYSLAWSLPPFFGW 1  MEL1_eriEur Erinaceus europaeus (hedgehog)
2 GCEFYAFCGALFGITSMMTLTAIALDRYLVITRPLASIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1  MEL1_sorAra Sorex araneus (shrew)
2 GCKFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRAALVLLGIWLYALAWSLPPFFGW 1  MEL1_loxAfr Loxodonta africana (elephant)
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRAALVLLVIWLYALAWSLPPFFGW 1  MEL1_echTel Echinops telfairi (tenrec)
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRTALVLLGTWLYALAWSLPPFFGW 1  MEL1_proCap Procavia_capensis (hyrax)
2 GCEIYAFCGALFGIASMMTLLAISLDRYLVITRPLAATGVVSRRRALLALPGIWLYALAwSLPPFFGW 1  MEL1_dasNov Dasypus novemcinctus (armadillo)
2 ACEFYAFCGALFGITSMITLMAIALDRYFVITRPLASIGVISKKKTGFILLGVWLYSLAWSLPPFFGW 1  MEL1_monDom Monodelphis domestica (opossum)
2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1  MEL1_smiCra Sminthopsis crassicaudata (fat-tailed dunnart)
2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGVVSKKKTGLILLGVWLYSLAWSLPPFFGW 1  MEL1_macEug Macropus eugenii (wallaby)
2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1  MEL1_smiCra Sminthopsis crassicaudata (dunnart)
2 GCQLYAFCGALFGITSMITLTVIALDRYFVITRPLASIGVISKKRALLILTGVWFYSLAWSLPPFFGW 1  MEL1_ornAna Ornithorhynchus anatinus (platypus)
2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVRVMSKKKALIILVGVWLYSLAWSLPPFFGW 1  MEL1_galGal Gallus gallus (chicken)
2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVGVTSKKKALIILVGVWLYSLAWSLPPFFGW 1  MEL1_taeGut Taeniopygia guttata (finch)
2 GCELYAFCGALFGIASMITLTVIALDRYFVITRPLASIGAMSTKKALLILSGVWLYSLAWSLPPFFGW 1  MEL1_anoCar Anolis carolinensis (lizard)
2 GCELYAFCGALFGITSMITLMVIAVDRYFVITRPLTSIGVMSKKRAVLILSGVWLYSLAWSLPPFFGW 1  MEL1_xenTro Xenopus tropicalis (frog)

Melanopsin MEL2 gene loss in mammals

A paralogous second melanopsin in non-mammalian vertebrates was found quite recently by Bellingham et al. It is still unclear whether this gene is present in chondrichthyes or lamprey because of the limited coverage of current assemblies. This evidently arose prior to divergence of teleost fish by a segmental duplication because some evidence of flanking synteny survives. It cannot have arisen by the whole genome duplication in fish because that would not have descended to frog, lizards, and birds. Instead all whole genomic duplication copies of fish melanopsins were lost. No sign of this second gene can be found in platypus, marsupials, or placental mammals -- yet another longstanding functional opsin gene lost in the mammalian crown group. 

Transmembrane, DRY loop, and Schiff base regions of human MEL1 are very similar to lizard MEL2:
LGTVILLVGLTGMLGNLTVIY  CVLVIGSIGITGNLLVLYAFY  DRYLVITRPLATFGVASKRR KASAIHNPIIYAITHPKYR   
FIINLAVSDFLMSFTQAPVFF  FIMNLAASDFLMSATQAPICF  DRYCVITKPLQSIKRSSKKR KASAIYNPIIYAIIHPRYR
FYAFCGALFGISSMITLTAIA  FYVFCGALFGITSMMTLLAIS
FVLLGVWLYALAWSLPPFFGW  IIIAFVWLYSLGWSVCPLFGW
LCCFVFFLPLLIIIYCYIFIF  FVFFIPLIIIFHCYLFMFLAI
IMLLVILLFVLSWAPYSAVAL  IAFVAIVVFVLSWSPYACVTL
SVPAVIAKASAIHNPIIYAIT  SVPAVIAKASAIYNPIIYAII

Reference set of MEL1 melanopsins

>MEL1_homSap Homo sapiens (human) Gq indel -GRID1 -WAPAL +LDB3 +BMPR1A 16961436 NM_033282 melanopsin OPN4 full
0 MNPPSGPRVPPSPTQEPSCMATPAPPSWWDSSQSSISSLGRLPSISPT 0 
0 APGTWAAAWVPLPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGNGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTLTSHTSNLSWISIRRRQESLGSESEV 0
0 GWTHMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGHEAETPGK 0
0 TKGLIPSQDPRM* 0

>MEL1_panTro chimp full
0 MNPPSGPRVPPSPTQEPSCMATPAPPSWWDSSQSSISSLGRLPSISPT 0
0 APGTWAAAWVPLPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGNGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTLISHTSNLSWISIRRRQESLGSESEV 0
0 GWTHMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGHEAETPGK 0
0 TKGLIPSQDPRM* 0

>MEL1_ponpyg Pongo pygmaeus full
0 MNPPSGPRVPPSPTQEPSCMATPAPPSWWDSSQSSISSLGQLPSISPT 0
0 APGTWAAALVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLMVIYTFCR 2
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATIGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGNGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTMISHTSNLSWISGRRRQESLGSESEV 0
0 GWTHMEAAAVWGAAQQANGRFLYDQGLEDLEAKAPPRPQGEEAETPGK 0
0 TKGLIPSQDPRM* 0

>MEL1_rheMac rhesus full
0 MNPPSGPRVPPSPTQEPSCMATPAPPSRWDSSQSSISSLGQLPSVSPT 0
0 AAGTWAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRGLRTPANMFIINLAISDFLMSFTQAPVFFASSLYKHWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATIGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETG 2
1 ALQTFGACKGSGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSHPYPSYRSTHRSTLISHTSNLSWISGRRRQESLGSESEV 0
0 GWTHMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGQEAETPGK 0
0 TKGLLPCKDSRM* 0

>MEL1_calJac marmoset frag
0 MNLPTGSRVLPSPTQEPSCMTTPAPPSRWDSSQSSISSLSQLPSISPT 0
0 AAGTWAAAWIPFPTVDVPDHAHYTLGTVILLVGVTGMLGNLTVIYTFCR 2
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFASSLYKRWLFGET 1
2 IALDRYLVITRPLATIGLASTRRAAFVLLGVWLYALAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGSGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSHPYPSYRSTHRSTLISHTSNLSWISGRRRQESLGSESEV 0
0 GWTHMEAAAAWGAAQQANGRSLYGHGLEDLEAKAPPRPQRQEAETPGK 0
0 TKGLIPSQDPRM* 0

>MEL1_tarSyr Tarsius syrichta poor coverage, ok frag
0 MNPASGPRVSPSSTQEPSCVASPALPSRWDSSQNSLSSLSQLPPISPT 0
0 AAGAWAAALVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTV  2
1 1
2 1
2 sAYVPEGLLTSCSWDYMSFTPSVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQNFGACKDGSESLWQRQRLQSEWKMAKIMFLVILLFVLSWAPYSAVALVAFAG 2
1 2
1 VAIAQHLPCLGVLLGVSRQHSRPYPSYRSTHRSTLSSQTSDLSWISGRRRQESLGSETEV 0
0 GWTDMEAADMWGDAQQVSVRFPYGQGLEDIETKASSRLQGQEAKTPGK 0
0* 0

>MEL1_otoGar bushbaby full
0 mhLPSAPRVPPSPAQVPSCVATPAPHSRWDSSQTSISSLGQLRPVSPT 0
0 ASGAWAAGWIPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRLRTPANMFVINLAVSDFLMSVTQAPVFFASSLYKQWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLTTVGVASKRRAALVLLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYTSFTPSVRTYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGSSESPRQRQRLQNEWKMAKITLLVILFFVLSWAPYTTVALVAFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGLLLGVSRQHSRPYPSYRFTHHSTLSSQASDLSWISGRRRQESLGSESEV 0
0 GWTDMEAAATWGAALQVSGQCPYSQGLEDMEAKGPLRPQGPETKTSGK 0
0 TKGLLPSLDPRM* 0 

>MEL1_micMar Microcebus murinus frag
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLASVGTASKRRAGLVLLGVWLYALAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYMTFTPSVRAYTMLLCCFVFFLPLLVIIYCYIFIFRAIRETGR 2
1 ALQTFGASKGTSESPRQQQRLQNEWKMAKIMLLVILLFLLSWAPYTAVALVAFAG 2
1 YAHILTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCVGVLLGVSRQHSRPYPSYRSTHRSTLSSQASDLSWISGRRRQESLGSE  0

>MEL1_tupBel Tupaia belangeri poor coverage, ok frag
0 AAGAQAAVRAPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 0
1  ACEGGGASPGQRQRQRLQSEWKMAKIALLVILLFVLSWAPYSTVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2

>MEL1_musMus Mus musculus (house mouse) gene models readthru error full
0 MDSPSGPRVLSSLTQDPSFTTSPALQGIWNGTQNVSVRAQLLSVSPT 0
0 TSAHQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 NRGLRTPANMFIINLAVSDFLMSVTQAPVFFASSLYKKWLFGET 1
2 GCEFYAFCGAVFGITSMITLTAIAMDRYLVITRPLATIGRGSKRRTALVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPQVRAYTMLLFCFVFFLPLLIIIFCYIFIFRAIRETGR 2
1 ACEGCGESPLRQRRQWQRLQSEWKMAKVALIVILLFVLSWAPYSTVALVAFAG 2
1 YSHILTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSGQRSHPSLSYRSTHRSTLSSQSSDLSWISGRKRQESLGSESEV 0
0 GWTDTETTAAWGAAQQASGQSFCSQNLEDGELKASSSPQVQRSKTPK 0
0 TKGHLPSLDLGM* 0

>MEL1_ratNor Rattus norvegicus (rat) 18001324,17652756,16687290,16186625,15509757,15073514,12904470,14500998,11834834
0 MNSPSESRVPSSLTQDPSFTASPALLQGIWNSTQNISVRVQLLSVSPT 0
0 TPGLQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 NRGLRTPANMLIINLAVSDFLMSFTQAPVFFASSLYKKWLFGET 1
2 GCKFYAFCGAVFGIVSMITLTAIAMDRYLVITRPLATIGMRSKRRTALVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYVTFTPLVRAYTMLLFCFVFFLPLLIIIFCYIFIFRAIRETGR 2
1 ACEGCGESPLRRRQWQRLQSEWKMAKVALIVILLFVLSWAPYSTVALVGFAG 2
1 YSHILTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 AAIAQHLPCLGVLLGVSGQRSHPSLSYRSTHRSTLSSQSSDLSWISGQKRQESLGSESEV 0
0 GWTDTETTAAWGAAQQASGQSFCSHDLEDGEVKAPSSPQEQKSKTPK 0
0 TKRHLPSLDRRM* 0

>MEL1_phoSun Phodopus sungorus (hamster) AY726733 mrna full
0 MDSPPGPTAPPGLTQGPSFMASTTLHSHWNSTQKVSTRAQLLAVSPT 0
0 ASGPEAAAWVPFPTVDVPDHAHYILGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMLIINLAVSDFLMSFTQAPVFFASSLYKKWLFGET 1
2 GCEFYAFCGAVLGITSMITLTAIALDRYLVITRPLATIGMGSKRRTALVLLGIWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYVTFTPQVRAYTMLLFCFVFFLPLLVIIFCYISIFRAIRETGR 2
1 ACEGWSESPQRRRQWHRLQSEWKMAKVALIVILLFVLSWAPYSTVALVAFAG 2
1 YSHILTPYMSSVPAVIAKASAIHNPIVYAITHPKYR 2
1 AAIAQHLPCLGVLLGVSSQRNRPSLSYRSTHRSTLSSQSSDLSWISAPKRQESLGSESEV 0
0 GWTDTEATAVWGAAQPASGQSSCGQNLEDGMVKAPSSPQAKGQLPS 0
0 LDLGMQDAP* 0

>MEL1_nanSpa Nannospalax ehrenbergi (mole-rat) AM748539 full
0 MNSPSGPRVPPGLAQKPSFMVTPVLPNQWISFQKNVSVGIQLPPASAT 0
0 ATGAQAASWVPFPTVDVPVHAHYTLGTVILLVGLTGMLGNLIVIYTFCR 2
1 SRGLRTRANMFTVNLAVSDFLMSFTQAPVFFASSLYKRWLFGEA 1
2 GCEFYAFCGAVSGITSMTTLTAIALDRYLVITRPLATIGVASKRRTALVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLFCFVFFLPLLIIIFCYIFIFKAIRETGR 2
1 ACEGCGESPQRRRQWQRLQNEWKMAKVALLVIFLFVLSWAPYSTVALVAFAG 2
1 YSHILTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 LAISQHLPCLGVLIGVSSQRSHPSLSYRSTHRSTLSSQASDLSWISGRKRQESLGSESEV 0
0 GWTDTEVTAAWGVAQEASGWSPYRHSLEDGEVKASPSPQGQEAKTSRK 0
0 TKGQLPSLNLRM* 0

>MEL1_cavPor Cavia porcellus frag
0     GLSPQVAGAQAAAWIPFPTVDVPAHAHYTLGAVILLVGLTGMLGNLTVIYTFCR 2
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKRWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAITLDRYLVITRPLATIGVASKRQAALVLLGVWLYALAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYVTFTPSVRAYTMLLFCFVFFLPLLVIIYCYIFIFRAIRETGR 2
1 LRSEWKMAKIALLVILLFVLSWAPYSAVALVAFAG 2
1   YRSTHRSTLSSQASDLSWISGRRRQESLGSESEV 0
1 YAHMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
0 GWTDTEA WGAAQQGSGQSPYDLVLEDWEARAPPKARVWGAGTPGK 0

>MEL1_oryCun rabbit frag
0 MNSPWGSRVPPGPAQEPRSTAPPSRWDGSESSISSPGQLTPGSPT 0
0 APGAQEAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMFIINLAVSDFLMSFTQAPVFFASSLYKRWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLAAVGMVSKKRAGLVLLGVWLYALAWSLPPLFGW 1
2 SAYVPEGLLTSCSWDYVTFTPSVRSYTMLLFCFVFFLPLLVIVYCYIFIFRAIRETGR 2
1  ACQGSHESPRQWRRLQSEWKMAKVALLVILLFLLSWAPYSTVALVAFAG 2
1 YAHSLSPYMNSVPAVIAKASAIHNPIIYAITHPKY 2
0 GWTDTEAAAAWGAALQLSGRYLCGQGLEDGEIKATPRRQGPEAETPRK 0

>MEL1_canfam dog
0 MNPPSGPGAQEPGCVATAASPGRWHGSPRSTVGLDQALPTGPT 0
0 AAGARAAAWAPFPTVDVPDHAHYILGTVILLVGLTGMLGNLMVIYTFCR 2
1 TRGLRTPSNMFIINLAVSDFFMSFTQAPVFFASSLHKRWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITHPLAAVGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMSFTPSVRAYTMLLFCFVFFLPLLVIVYCYVFIFRAIRETGQ 2
1 ALQTFRACEGGARSPRQRQRLQREWKMAKMELLVILLFVLSWAPYSAVALTAFAG 2
1 YSHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAQHLPCLGVLLGVSGQRTGPYASYRSTHRSTLSSQASDLSWISGRRRQASLGSESEV 0
0 GWMDTEAAAVWGAAQPAGGRFLCTQGLEDAEAKAPLRPRGQAVETPGK 0
0 TKGRLPSLDPSREGAHWLPPSPDPG* 0

>MEL1_felCat cat full 
0 MNPPSGPRT QEPSCVATPASPSRWDGYRSSTSSLDQPLPISPT 0
0 AARAQAAAWIPFPTVDVPDHAHYTLGTVILLVGLTGILGNLMVIYTFCR 2
1 SRGLRTPANMFIINLAVSDFFMSFTQAPVFFASSLHKRWLFGEA 1
2 GCEFYAFCGALFGITSMITLMAIALDRYLVITHPLATIGVVSKRRAALVLLGVWLYALAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYMSFTPSVRAYTMLLFCFVFFLPLLVIVYCYIFIFRAIRETGQ 2
1 ALQTFRACEGGGRSPRQRQRLQREWKMAKIELLVILLFVLSWAPYSIVALMAFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAQHLPCLGVLLGVSGQHTGPYASYRSTHRSTLSSQASDLSWISGRRRQASLGSESEV 0
0 GWMDTEAAAVWGAAQQVSGRFPCSQGLEDREAKAPVRPQGREAETPGQ 0
0 TKGLLPSQDPRM*

>MEL1_bosTau (cow) XM_593123 mrna VMPT possible full 
0 MNPPSGPRAPLGPVQESSCLATPASSSRWDSSRSSASSLGHPPSISPT 0
0 AVRAQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFASSLYKQWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYVSFTPSVRAYTMLLFCFVFFLPLLIIIYCYIFIFKAIRETGQ 2
1 ALQTFGTCEGGSECPRQRQRLQNEWKMAKIELLVILLFVLSWAPYSTVALMGFAG 2
1 YAHILTPYMNSVPAVIAKASAIYNPIIYAITHPKYR 2
1 LAIAQHLPCLGVLLGVSGQRTGLYTSYRSTHRSTLSSQASDLSWISGRRRQASLGSESEV 0
0 GWMDTEATAAWGAGQQVSGWSPCSQRLDDVEAKALPRPQGRDSEAPGK 0
0 AKGLLPNLDARM* 0

>MEL1_susScr Sus scrofa (pig) VPT in transcript CX057760? 
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFASSLYKQWLFGEA 1 
2 GCEFYAFCGAVFGITSMITLTAIALDRYLVITYPLATVGMVSKRRAALVLLGVWLYALAWSLPPFFGW 1
2 SAYVPERLLTSCSWDYVSFTPKVRAYTMLLFCFVFLLPLLVIIYSYIFIFKAIRETGr 2

>MEL1_equCab Equus caballus (horse) full
0 MNPPSEPQVPLGLAQEPGCVATPASPSRWSGSRSSTSSLGQPLPVGPT 0
0 AAGAQADAWVPFPTVDVPDHAHYTMGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFASSLYKQWLFGKA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATVGVVSKRWAALVLLGIWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLMCFVFFLPLLVIVYCYVFIFRAIRETGR 2
1 ALQTFGAWEGGGECPRQRQRLQSEWKMAKIVLLVILLFVLSWAPYSVVALVAFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAIIHPKYR 2
1 MAIAQHLPCLGVLLGVSSQRTRPYTSYRSTHRSTLSSQGSDLSWISGRRRQASLGSESEV 0
0 GWMDTEAAAVWGAAQQMSGWSPCGQGLEDMEAKAPPRPQGWEGEALRK 0
0 IKGLLPSLDPRM* 0

>MEL1_eriEur hedgehog frag
0 MALSLGPRVPTSQALDPSCMDTPASPSRWDSSQNSTSSLAQLPLISLT 0
0   QATASAPFPTVNVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMFIINLAVSDFLMSFTQTPVFFASSLYKQWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRVALVLLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMRFTPSFRAYTMLLFCFVFFLPLLVIIYCYIFIFRAIRETGQ 2
1 ALQTFRACEGSCDFPRQQQRLQSEWKMAKIILLVILLFVLSWAPYSTVALMAFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAQHLPCLRVLLGVSGQRDRPYTSYRSTHRSTLSSQISDLSWVSRRRRQASLGSESEV 0
0 GWTDTEVAAVWGT  MSGHFPCGQGLDDMEAKAAHNPRGLEAETPGK 0
0 IKGLLPSLDPQM* 0

>MEL1_loxAfr (elephant) ok frag
0 MNPPWGPRVPSGRAQEPSCVATPASASRWNSSRASASSLGELPPSSPT 0
0 AARAHTAAWDPFPTVDVPDHAHYTLGAVILLVGLMGMLGNLMVIYIFFR 2
1 SRGLRTPANMFIINLAVSDFLMSFTQAPVFFASSLYKRWLFGEA 1
2 GCKFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRAALVLLGIWLYALAWSLPPFFGW 1
2 sAYVPDGLLTSCSWDYMSFTPSFRAYTMLLCCFVFFLPLLVIIYCYIFIFKAIRETGR 2
1 ALQTFGACEGASESPRQWQRLQSEWKMAKIALLAILLYVLSWAPYSTVALVAFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAQHLPCLGVMLGVSGQRTRPYTSYHSTLHSTLSSQASDLSWISGRRRQASLGSESEV 0
0 GWTDTEAAAAWEGAQQVSGQASCSQALQNLEANTPPRPQGWGPETPRK 0
0 * 0
  
>MEL1_echTel (tenrec) frag
0 MNLPSGSRVPSGPAQEPSHVATAASASRNSGSSLDVPPPSSPT 0
0 AARAPTAASAPFPIVDVPDHVHYTLGTVILLVGLTGMLGNLMVIYTFCR 2
1 SRSLWTPANMLIINLAVSDFLMSFTQASVFFTSSLYKQWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRAALVLLVIWLYALAWSLPPFFGW 1
2 SAYVPDGLLTSCSWDYMSFTPSVRAYTMLLFCFVFFLPLLVIIYCYIFIFRAIRETGR 2
1 ALQTFGACEGSRDSPRQRQRLQSEWKMAKIALLVISLFVLSWAPYSTVVLVAFA 2
1  2
1  0
0 GWTDTEVTATSGYTQQVSGRCPRGQDLESMDANPTPRPRGWETETAQK 0
0 IKGLPLSLNPQA* 0

>MEL1_Procavia_capensis (hyrax) ok frag
0 MNPPWGPRVSSRPAQEPSCMSTPASAGRWDNSQATASSLAELPPSSPT 0 
0 EARTQTADWVPFPTVDVPDYAHYTLGTVILLVGLTGVLGNLMVIYIFFR 2
1 SRGLRTPANMFIINLAISDFLMSLTQAPVFFASSLYKRWLFGEA 1
2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRTALVLLGTWLYALAWSLPPFFGW 1
2 SAYVPDGLLTSCSWDYKSFMPSARTYTMLLCCFVFFLPLLVIIYCYVFIFKAIRETGR 2
1 ALQTFGACEGASETPRQWQRLQSEWKMAKIALLAILLYVLSWAPYSTVALVGFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAQHLPCLGVLLGVSDQHTRPYTSYRSTHHSTLSSQASDISWISGRRRQASLGSESEV 0
0 GWTDTEAAAAWEGAQQVSGRASCSQVLESMEANTPPRPQGWGPETPRK 0
0 * 0

>MEL1_dasNov Dasypus novemcinctus so-so frag sloth weak frag
0 MDPPSGPSVLTGGPSWGATPASPSGWDGSRSSTSSLHGRPPTSPL 0
0 AAGARAAAWDPFPTADVPAHAHYTLGSVILLVGLTGTLGNLTVIYTFCR 2
1 SRGLWTTANTFVINLAVSDLLMSITQAPVFFASSLYKQWIFGET 1
2 GCEIYAFCGALFGIASMMTLLAISLDRYLVITRPLAATGVVSRRRALLALPGIWLYALAwSLPPFFGW 1
2 SAYVPEGLLTSCSWDYSSFSPSARAYTMLLFCCVFLLPLLAIVYCYICIFRTIRETRR 2
1 ALQNLRAGEGASPLPGQR LQSEWRTAKITLLVVLLYVLSWAPYSTVALVAFAG 2
1 YAHVLTPYMNSVPAVIAKASAIHNPIVYAITHPEYR 2
1 MAIAQHLPCLGLLLGVLGHRPRPGSSPGSTRCSAHSGQASGLSWISRQRRRASLGSKDEV 0
0 GWEDVEAAAASGAAGQESGRSPRAQDLEHMEAEAARWPSWEAEPEK 0
0 * 0

>MEL1_monDom Monodelphis domestica (opossum) Gq 0.0.1.2.2.1.1.1.0.0 indel -GRID1 -WAPAL +LDB3 +BMPR1A full 
0 MNPSPMLRGLSCPAQDTNCTKIMASMSEWNNTEEDAYHLVDLPSIAPT 0
0 AVVLPPSSQNIFPTADVPDHAHYTIGATILAVGFTGVLGNLLVIYTFCR 2
1 SHSLRTPANMFIINLAISDFFMSFTQAPVFFASSMYKRWIFGEK 1
2 ACEFYAFCGALFGITSMITLMAIALDRYFVITRPLASIGVISKKKTGFILLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFRAIQDTNK 2
1 AVHSIGSGESTASPRHCQRMKNEWKMAKIALVVILLYVLSWAPYSTVALVAFAG 2
1 YSHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2
1 MAIAQNFPCLRALLCVRHPRTRSFSSYRFTRRSTMTSQASDISWLPRGRRQLSLGSESEI 0
0 GWNNMEAGTTSLTSRNQQGSCRMDQETMETRELAAIAKAKGRSWETLEK 0
0 TLEEMDDSSLLEVSVDMEQ* 0

>MEL1_smiCra Sminthopsis crassicaudata (fat-tailed dunnart) 17785267 full
0 MNPSPMLRHLSCPAQDSNCTKIMASISEWNNTEVDAYHLVDLPPITPT 0
0 AVVLPPYSQKVFPTADVPDYAHYTIGATILVVGFTGVLGNLLVIYTFCR 2
1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFASSLYERWIFGEK 1
2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYTTFTPSVRAYTILLFCFVFFIPLTVIIYCYIFIFRAIKDTNK 2
1 AVQNIGSSEHTPSLRHFQRMKNEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2
1 YSHVLTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2
1 MAIAQNFPCLRAVLGIRHPRTQSFSSYRFTHRSTTASQASDISWQSRGRRQLSLGSESEA 0
0 GWNNIETGLTLRSLEGSCGMDEETMDTRELSASTKAKGQSWETLAK 0
0 TLEEMDDLSLLEAGTLLSSLDLQI* 0

>MEL1_macEug Macropus eugenii high id frag
0 AVVLPPHSRNIFPTADVPDHAHYTVGAIILVVGFTGVLGNLLVIYTFCR 2
1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFANSLYKRWIFGEK 2
2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGVVSKKKTGLILLGVWLYSLAWSLPPFFGW 1
2 AYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFKAIQDTNK 2
1 ALQNIRSSESTASPRHFQRMKSEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2
1 SHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2

>MEL1_ornAna (platypus) ok frag
0 0
0 GEASLPSSYHAFPTVDVPDHAHYTIGAIILAVGITGMLGNFLVIYTFCR 2
1 SRSLRTPANMFIINLSISDFFMSLTQAPVFFASSLHKRWIFGEK 1
2 GCQLYAFCGALFGITSMITLTVIALDRYFVITRPLASIGVISKKRALLILTGVWFYSLAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYMTFTPPVRAYTMLLFCFVFFIPLIMIIYCYFFIFRAIRGTNK 2
1 AVETIGSDDCRGSQRQCQRMKNEWKTAKIALMVILLYVISWCPYSVVALVAFAG 2
1 YSHLLTPYMNSVPAVIAKSSAIHNPIIYAITHPKYR 2
1 MAITKYIPCLGPLLRVSRQDSRSSSHYASSRRSTVTSQSLDGSWLPGRRRPLSSASDSES 0
0 GWTDTAADAGSASSRAASRQVSYRMSQGPTEHCDLRAKVKPKSWEVGSFQK 0

>MEL1_galGal Gallus gallus (chicken) -GRID1 -WAPAL +LDB3 +BMPR1A OPN4m  EU124633 EU124632 full
0 MDLPPRAPT 0
0 KMTVKDVRGAFPTVDVPDHAHYTIGTVILIVGITGTLGNFLVIYAFCR 2
1 SRTLQKPANIFIINLAVSDFLMSITQSPVFFTNSLHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVRVMSKKKALIILVGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFEAIKKANK 2
1 SVQTFGCKHGNRELQKQYHRMKNEWKLAKIALIVILLYVISWSPYSVVALVAFAG 2
1 YSHVLTPFMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 TAIATYVPCLGFLLRVSPKESRSFSSYPSSRRTTITSQSSETSGLQKGKRRLSSISDSES 0
0 GCTDTETDITSMISRPASSQVSYEMGEDTTQTSDLGGKPKVKSHDSGIFgk 0
0 aVVDADEIPMVEINDTEHSATST 0
0 CKTSEKCNVEEIQ 0
0 RSESLSGIGLREGESRrRTSASQIPSIIITYSNVQGVELpSGYSAGFLHPKNKSHiQNKSSNS* 0

>MEL1_taeGut Taeniopygia guttata frag 25% melatonin receptor
0 0
0 KMTVQDVPRAFPTVDVPDHAHYTIGVVILIVGITGTLGNFLVFYAFCR 2
1 SRSLQTPANILIINLAISDFLMSITQSPVFFTSSLYKHWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVGVTSKKKALIILVGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLFCFVFFIPLIAIIYSYVSIFEAIKKANK 2
1 SIQTFGCKRGNREFQKQYQRMKNEWKMAKIALIVILFFVISWSPYSVVALVAFAG 2
1 YSHVLTPFMNSIPAVIAKASVIHNPIIYAITHPKYR 2
1 KAIATYVPCLGPLLRVSPKDSRSFSSYHSSRRATISSQSSEISGLQERKRRLSSLSDSES 0
0 GCTETETDTPSMFSRLARRQISYKTDKDTTQTSDIRAKLTSQDSGWGVAK 0
0 0
0 KSESLNSIGQRREFHQGSSSAQIPSITITCSSVQGIELPSRYNSGFLYPKSSSHKQNKKSSS* 0

>MEL1_anoCar diverged frag VG* option supports chicken oddity, mammalian is the one with the weird new terminal exons
0 ERTMFNLPDPFPTVDVPTHAHYTIGAVILVVGITGTLGNLLVIYVFFR 2
2 GCELYAFCGALFGIASMITLTVIALDRYFVITRPLASIGAMSTKKALLILSGVWLYSLAWSLPPFFGW 1
2 sAYVPEGLLTSCSWDYITFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFIAIKNSNR 2
1 RAVQRTNSDNSKEGQKLYQKLKNEWKMAKVALIVILLYVISWSPYSVVALVAFAG 2
1 YSHLLTPYMNSVPAVIAKASVIHNPIIYAIVHPKYR 2
1 MAIAKFLPCLGSLLRVPRKDSSYPSTRRPTVTSQSSDINGVPRGHRRLSSVSDSES 0
0 DWTDTEADISSQNSRVASGSISYRIYEDTTETIKVKSKMRSHDSGIFER 0
0 TSVDADDISMVEVNIRDYCFSI 0
0 TGEDLNAFGWRREESYSGPSTSSQIPSIIVTFSNVQRTDLPLESSSGALCSRNSSYSWEKDSNS 0

>MEL1_xenTro Xenopus tropicalis (frog) Gq 0.0.1.2.2.1.1.1.0.0 indel -GRID1 -WAPAL +LDB3 +BMPR1A 596 aa 000 nm 16856781 DQ384639 melanopsin OPN4m  
0 MNYQSVRKGITCPPQDANCSRILESLNSWNNSEVNSYKLVELPPIVTT 0
0 ETPQYEIHHVYPTVDVPDHVHYVVGAVILAVGITGMLGNFLVIYAFCR 2
1 SRSLRSPANMFIINLAITDFLMSVTQAPVFFATSLHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIAVDRYFVITRPLTSIGVMSKKRAVLILSGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFCFVFFIPLFIIIYCYIFIFKAIKNTNR 2
1 AVQKIGTDNNKESHKQYQKMKNEWKMAKIALIVILLYVVSWSPYSTVALLAFAG 2
1 YASILTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAKYIPCLGSLLRVKRRDSRSYSSYPSSRRSTVTSHCSQSSDVGGHPKLKNHLPSVSDSES 0
0 GWTDTEADSSVNSRPASRQVSYEMGKDTTETNDLKSKAKLKSHDSGIFEK 0
0 TSMDADDISLVELGTVDRSSPIM 0
0 ANKHLNGLGQRKGDSFTRRSPSSRIPSIVVTHSNHQGSPAAVRHNSTLPGIKVSNSQDREKELKRQIEKVKQYVPIVTITSDTENSTGGFSNELLPANTS* 0

>MEL1a_danRer Danio rerio (zebrafish) Gq 0.0.1.2.2.1.1.1.0.0 indel - +USP54 +LDB3 +BMPR1A no_ref AY078161 melanopsin OPN4m  
0 MMSGAAHSVRKGISCPTQDPNCTRIVESLSAWNDSVMSAYRLVDLPPTTTTTTSVA 0
0 MVEESVYPFPTVDVPDHAHYTIGAVILTVGITGMLGNFLVIYAFSR 2
1 SRTLRTPANLFIINLAITDFLMCATQAPIFFTTSMHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLMVIAVDRYFVITRPLASIGVLSQKRALLILLVAWVYSLGWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFIFVFFIPLIVIIYCYFFIFRSIRTTNE 2
1 AVGKINGDNKRDSMKRFQRLKNEWKMAKIALIVILMYVISWSPYSTVALTAFAG 2
1 YSDFLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 LAIAKYIPCLRLLLCVPKRDLHSFHSSLMSTRRSTVTSQSSDMSGRFRRTSTGKSRLSSASDSES 0
0 GWTDTEADLSSMSSRPASRQVSCDISKDTAEMPDFKPCNSSSFKSKLKSHDSGIFEK 0
0 SSSDVDDVSVAGIIQPDRTLTN 0
0 AGDITDVPISRGAIGRIPSIVITSESSSLLPSVRPTYRISRSNVSTVGTNPARRDSRGGVQQGAAHLSNAAETPESGHIDNHRPQYL* 0

>MEL1b_danRer Danio rerio (zebrafish) Gq 0.0.1.2.2.1.1.1.0.0 indel - +USP54 +LDB3 +BMPR1A 4 no_ref genome melanopsin OPN4m  
0 VAMVQDVRHPFPTVDVPDHAHYTIGSVILAVGITGMVGNLLVMYAFCK 2
1 SRSLRTPANMFIINLAVTDFLMCVTQTPIFFTTSLHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLMIIAVDRYFVITRPLASIGVMSRKRALLILSAAWAYSMGWSLPPFFGW 1
2 GAYVPEGLLTSCSWDYMTFSPSVRAYTMLLFTFVFFIPLFVIIYCYFFIFKAIRETNR 2
1 AVGKINGEGGPRDSIKKIHRMKNEWKMAKIALIVILLYVISWSPYSCVALTAF 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 SAIAKYIPCLGVLLCVPRRDRFSSSSFISTRRSTLTSQSSETSSNLHRAGKARLSSVSDSES 0
0 GWTDTEADLSTASSRPASRQVSSEIRKDLCDIKHSSSLRLKVKSRDSGIFDR 0
0 0
0 QNDVSEKADEKRPLVRIPSIIVTSETCPAVLPAGHSSRLIPGAPAVTDS* 0

>MEL1_rutRut Rutilus rutilus (roach minnow)
0 MSHHSSWRGHHCAPGDNNCTAGFKESLGSKNYKLLHVPFHGPT 0
0 HSHHHEPPHPFPTVDVPDHAHYIIGAVILIVGITGVIGNALVIYVFCR 2
1 SRTLRTAGNMFVVNLAVADFFMSLTQSPVFFAASLHRRWIFGER 1
2 ICELYAFCGALFGICSMMTLTAIAADRCLAITQPLALVGNVSRRKAGAVLAVVWLYSLGWSLPPFFGW 1
2 SAYVPEGLQTSCSWDYMTFTPSVRAYTILLFIFVFFIPLGIIVSCYVGIFQAIRAMG 2
1 KEIRELDCGETQKVYERMQNEWKMAKIALLVILLFVISWSPYSVVALTATAG 2
1 YSHLLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 AAIARYIPVLRTILRVKEKELRSSFSSGSVSSRRPTLSSQCSLGVSIGNAARANGRWGKKRLSSASDSDS 0
0 CWTESEADGSSVSSLTFGRRVSTEISTDTVILSPGSSNSTASGQKSEKAHKVVSVPVPSITFETDSADESLSDGKALLLGGN* 0

>MEL1_astBur Astatotilapia burtoni cichlid EU523855
0 MSHIHSSRKSALCPLGGNCTEHHHQVRMSKLLHLPDHAPT 0
0 QSARAQSHLFPTVDVPDHAHYIIGSVILLVGVTGMLGNALVIYVFCR 2
1 SRTLRSPSNLLVVNLAAADFLMSLTQSPVFFVASLHHRWVFGEL 1
2 ACELYAFCGALFGITSMITLTAIAVDRCLAITRPLAMLGGVSHQRVLVVVAVVWLYSLGWSLPPFFGW 1
2 SAYVPEGLQTSCSWDYMSFTAAVRTYTLLLFSFVFFIPLAVIAACYFAIFRAVQQAG 2
1 REIEQLNCGEVNKAYERLRSEWRMAKVALVVILLFIVSWSPYSVVALTATAG 2
1 YAHLLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 AAIGHYVPFLRSVLRLQEKDLRSSFSSSATSSRCTTFTSSPKGRLNASVRTNGHQAQNRLSSVSDSKSCWMESDADGSSRRSERQAFSEATANPLDSTTPRQHVGHTDASSSDGAVLEAKLPL* 0

>MEL1_takRub Takifugu rubripes (teleost) Gq 0.0.1.2.2.1.1.1.0.0 indel - +USP54 +LDB3 +BMPR1A 555 aa 000 nm no_ref genome melanopsin OPN4m  
0 MNFGKSALQPPAQQSVVSCGGGGPEPNCTLRLAVTVMMSVRLAELQLHAST 0
0 LQVAMVRPFPTVDVPDHAHYTIGSVILVIGITGMIGNFLVIYAFCR 2
1 SRSLRTPANMFIINLAVTDLLMCVTQTPIFFTTSMYKRWIFGEK 1
2 GCELYAFCGALFGICSMITLTVIAIDRYFVITRPLTSIGVLSRKRAFVILMTVWIYSLGWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFSPSVRAYTMLLFIFVFFLPLFIIIYCYFFIFRAIRATNK 2
1 AVGKVNGSVHSHSRRRESVKNFQRLQNEWKMAKIALMVILLYVISWSPYSCVALTAFAG 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 LALAKYIPCLGFLLCISPHELQSTSSSFMSLRRSTVTSQTSDISGQFRPQSKPRRSSASDSES 0
0 CLTDTEADLSSMGSRPASRQVSCDISRDTTELPEYKPASSFNSKVKSPDSGIFEK 0
0 TSFDFDASMAASRERSSIPN 0
0 SGEFPEGHVMRRTLARIPSIIITSESSHFLPNGRKASSTTCIANGSDIKVGPR* 0

>MEL1_gasAcu Gasterosteus aculeatus (stickleback) Gq 0.0.1.2.2.1.1.1.0.0 indel - - +LDB3 +BMPR1A 556 aa 000 nm no_ref genome melanopsin OPN4m  
0 MNAGESELLLPTQQSILPCGDHEPNCPVAQAETLALSAASANGSA 0
0 VQVAMVSRAPHPYPTVDVPDHAHYTIGSVILAIGITGIIGNVLVIYAFSK 2
1 SRSLRTPANMFIINLAITDLLMCVTQAPIFFTTSMHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLTVIALDRYFVITRPLTSIGMMSRRRALLILMGAWTYSLGWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFIFVFFLPLFIIIYCYFFIFRAIRVTNR 2
1 AVGKMNGSIHSHGSGRDSTKNFHRLQNEWKMAKIALIVILLYVVSWSPYSAVALTAFAG 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 IALAKYIPFLGVLLCVPPRELRSASSSFRSTRRSTVTSQTSDVSSQQRRQGSRNSRLSSASDSES 0
0 CLTDTEADGSSVGSRPASRQVSCDIGRDTAELPEFKPSSSFKSKMKSHDSGIFEK 0
0 SYDTDISMAGVSERGSIPN 0
0 QTDFAEGRDRRSTIGRIPSIVITSETSPFLPTGRNGSCNGRPKTANSSHPGAGSG* 0

>MEL1_oryLat Oryzias latipes (medaka) Gq 0.0.1.2.2.1.1.1.0.0 indel - +USP54 +LDB3 +BMPR1A 504 aa 000 nm no_ref genome melanopsin OPN4m  
0 LQVAMVPQTFHPFPTVDVPDHAHYTIGSVILAIGITGIIGNFLVIYAFSR 2
1 SRSLRTPANMFIINLAITDLLMCVTQSPIFFTTSMHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLTVIAIDRYFVITRPLTSIGVLSRKRALLILSAAWAYSLGWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFIFVFFLPLFIIIYCYVFIFRAIRSTNR 2
1 AVGKINGNTRDAVKSFNRLQNEWKMAKIALIVILLYVISWSPYSTVALTAFAG 2
1 YADMLTPYMNSIPAVIAKASAIHNPIIYAITHPKYR 2
1 MALAKYIPGLGVLLCIHPKDLRSASSSFVSTRRSTVTSQSSDISSQLRRQSTFKSRLSSLSDSES 0
0 GLTDTEADLSSLSSRPASRQVSCEISRDTAELPDFKHTSSFKAKLKNNDSGIFEK 0
0 TSFDTVSIGGVSEHNSIPS 0
0 NRDFGDGNVTRATIGRIPSIVVTSEMSPFLPVGRNGSRTNRSKMANSSAGAGPV* 0

>MEL1a_calMil Callorhinchus milii (elephantfish) Gq 0.0.1.2.2.1.1.1.0.0 indel - - - - 369 aa 000 nm no_ref genome melanopsin OPN4m  
0 ASVTDAQHHHMFPTVDVPDHAHYIIGATILAVGVTGMVGNFLVIYAFLR 2
1 SRSLRTPANTFIINLAATDFLMSVTQSPIFFITSIHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIALDRYFVITRPLASIGVLSHRRAGLIILSLWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFCFVFFIPLGVIIYCYIFIFRAIKSTNK 2
1 KVGGSTNRESQKQHQRMKNEWKMAKIALIVILLFVISWSPYSTVALTAFAG 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAKYVPLLGLLLRVSRRDSRTSGQYYSTRRSTLTSQTSDLSGYPRGKGRLSSASDSES 0

>MEL1b_calMil Callorhinchus milii (elephantfish) Gq 0.0.1.2.2.1.1.1.0.0 indel x x x x 113 aa 000 nm no_ref EB687868 melanopsin OPN4m  
1 SKSLRTPANMFIINLAISDFFMSATQPPVFFVTSLHKRWIFGEK
2 GCKLYAFCGALFGITSMITLMAISIDRYWVITKPLQSISSTTTKKNTLKVIILVWLYSLAWSLPPLLGW 1

>MEL1_petMar Petromyzon marinus (lamprey) first exon uncertain frag
0 MAPSIVPLMRKLSCPLSRKTPPKHNVSTSMFDGGDGVLGVIGSIP 0
0   VSFLPSQREKFPMLDIPHHVHHTIGSVVIAIGFTGIIGNFLVIYVFCR 2
1 SKSLRSPANIFIINLAFADFFMSITQTPIFFVTSLHKRWIFGEK 1
2 GCELYAFCGALFGIASMVTLMVIATDRYLVLTRPLASIGAMSKRRAMYITAAVWFYSLAWSLPPFFGW 1
2 SAYVPEGLMTSCTWDYVTFTPAVRSYTMLLFCFVFFIPLIVIIFCYVRIFAAIKNTNR 2
1 AVKTLGDAHDSKESQKQQQRMNAEWKLAKIALIVILLYVVSWSPYSCVALVAWAG 2
1 YADMLTPYMNSVPAIIAKASAIHNPIVYAITHPKYR 2

>MEL1a_braFlo Branchiostoma floridae (amphioxus) Gq 0.0.1.2.2.1.1.1.0.0 indel - - - - 709 aa 000 nm no_ref genome melanopsin Amphi-mop 12 exons +tandem dup assembly error  
0 MTELPSFQPPTNSTEEENAVFPTALTEWISE 0
0 VGNQVGEAALKLLSGEGDGMEVTPTPGCTGNASVCNGTDSGGGVVWDIPPLAHYIVGTAVFCVGCCGMFGNAVVVYSFIK 2
1 SKGLRTPANFFIINLALSDFLMNLTNMPIFAVNSAFQRWLLSDF 1
2 ACELYGFAGGLFGCLSINTLMAISMDRYLVITKPFLVMRIVTKQR 0
0 VMFAILLLWIWSLVWALPPLFGWSAYVPEGF 1
2 GTSCTFDYMTPKLSYHIFTYIIFFTMYFIPMGVIIYCYYNIFATVKSGDKQFGKAVKEMAHEDVKNK 0
0 AQQERQRKNEIKTAKIAFIVITLFLSAWTPYAVVSALGTLGYQDLVTPYLQSIPAVFAKSSAVYNPI 1
2 VYAITHPKFRAAVKKHIPCLSGCLPADEEETKTKTRGATTTASMSMTQTTAPTV 0
0 HDPQASVHSGSSVSVDDSSGVSRQDTMMVK 0
0 VEVDNRMEKAGGGAADTAPKDGTSVPTVSAQIEVRPSGNVNTKAEVIPSPQSAAVAHGASASPVPK 0
0 VAELSSSVSLESAAIPGKIPTPLPSQPIAAPIERHMAAMADDPPPKPRGVATTVNVRRSESGYERSQDSLRKK 0
0 AVSETRSRSFNSTKDHFASERQTSTTLNQPRDMYSGDMVKKTRQSPEKQEYDNPAFDAGIAEIDTDSENETEGSYDMLSVRFQAMAEEPPVETYRKASDMSINLGKASLMLTEAHDETVL* 0

>MEL1a_braBel Branchiostoma belcheri (amphioxus) Gq 0.0.1.2.2.1.1.1.0.0 indel x x x x 707 aa 000 nm 15936279 AB205400 melanopsin Amphi-mop  
0 MTEIPSFQPPINATEVEEENAVFPTALTEWFSE 0
0 VGNQVGEVALKLLSGEGDGMEVTPTPGCTGNGSVCNGTDSGGVVWDIPPLAHYIVGTAVFCIGCCGMFGNAVVVYSFIK 2
1 SKGLRTPANFFIINLALSDFLMNLTNMPIFAVNSAFQRWLLSDF 1
2 ACELYGFAGGLFGCLSINTLMAISMDRYLVITKPFLVMRIVTKQR 0
0 VMFAILLLWIWSLVWALPPLFGWSAYVSEGF 1
2 GTSCTFDYMTPKLSYHIFTYIIFFTMYFIPGGVMIYCYYNIFATVKSGDKQFGKAVKEMAHEDVKNK 0
0 AQQERQRKNEIKTAKIAFIVISLFMSAWTPYAVVSALGTLGYQDLVTPYLQSIPAMFAKSSAVYSPI 1
2 VYAITYPKFREAVKKHIPCLSGCLPASEEETKTKTRGQSSASASMSMTQTTAPV 0
0 HDPQASVDSGSSVSVDDSSGVSRQDTMMVK 0
0 VEVDKRMEKAGGGAADAAPQEGASVSTVSAQIEVRPSGKVTTKADVISTPQTAHGLSASPVPK 0
0 VAELGSSATLESAAIPGKIPTPLPSQPIAAPIERHMAAMADEPPPKPRGVATTVNVRRTESGYDRSQDSQRKK 0
0 VVGDTHRSRSFNTTKDHFASEQPAALIQPKELYSDDTTKKMARQSSEKHEYDNPAFDEGITEVDTDSENETEGSYDMLSVRFQAMAEEPPVETYRKASDLAINLGKASLMLSEAHDETVL* 0

>MEL1b_braFlo Branchiostoma floridae (amphioxus) Gq 0.0.1.2.2.1.1.1.0.0 indel - - - - 402 aa 000 nm no_ref genome melanopsin Amphiop6  
0 MSPNLTNTSLLPNRTDRPELSPADVTMQLVFGSMMLVFGLIGVVGNAVALYAFCR 2
1 SRSLRRPKNYLIANLCLTDMVVCLVYSPIIVTRSLSHG 2
1 LPSKESCIVEGFVVGLGSIVSICSLAGIAVERYVTITQPIKSLSILTHRALLGAVSAVWVYAFLLAFPPLVGWGRYVSEESKISCTFDYLSTDDATRAHVIVLVIGAFGLPFS
VITYCYVRSFATVRKCTKERKQMSPLAKSDSRSEVKAAVNSFVITTSFCLCWCPYAVVATMGVSGFTVHSHAVFIAALLAKLSVLFNPVAYVLSIP 1
2 NSNVNIESTELTVPYSASRESCLLSRAATERLAGRSPSLTDIVREFGLQQTASHRE

>MEL1b_braBel Branchiostoma belcheri (amphioxus) Gq 0.0.1.2.2.1.1.1.0.0 indel x x x x 402 aa 000 nm 12435605 AB050611 melanopsin Amphiop6  
0 MSSNLTNVSLVANRTDQTELSPTDVTMQLIFGSMMLVFGLIGVVGNVVALYAFCR 2
1 TRSLRRPKNYVVANLCLTDMFVCLVYCPIVVSRSFSHG 2
1 FPSKESCIVEGFMVGVGSIASICSLAAIAVERYLSVTQPLKSLTILTQRKLLVAVLTVWVYSLLLAFPPLVGWGRYVREETYISCTFDYLSTDDATRAYVITLVMGAFGFPLL
TIAYCYIRVFTTARKHAEERKFMSPLKRPESRTEIKTAVTACVITTSFCLCWCPYAVVATLGISGVSVQQQTVFSAALLAKLTVIINPIVYVLSIPNFRKALFAQEREKYASED
VVLTSLPGKTRRMKKVERSQSSNSNVVIEVKESSMAYSTSRESCLLSRAATKRLAGKTKSIVDLVDEFGLQETAPHKESLV* 0

Reference set of MEL2 melanopsins


>MEL2_galGal Gallus gallus (chicken) Gq 0.0.1.2.2.1.1.1.0.0 indel +GRID2 +SMARCAD1 -PGDS -SEC24B +COL25A1 544 aa 000 nm 17977531 NM_204625 full
0 MGTQPHSVTKSEIPDHVLYTVGTCVLVIGSIGIIGNLLVLYAFYS 2
1 NKKLRTPQNFFIMNLAVSDFLMSASQAPICFVNSLHREWILGDI 1
2 GCDLYAFCGALFGITSMMTLLAISVDRYLVITKPLRSIQWTSKKRTIQIIAAVWLYSLGW 1
2 SVAPLLGWSSYVPEGLMISCTWDYVTYSPANRSYTMILCCCVFFIPLIIILHCYLFMFLAIRSTGR 2
1 DVQKLGSCSRKSFLSQSMKNEWKLAKIAFVVIIVYVLSWSPYACVTLIAWAG 2
1 RGNTLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2
1 KTIHNAVPCLRFLIRISKNDLLRGSINESSFRTSLSSHQSLAGRTKNTCVSSVSTGEA 0
0 NWSDVELDTVEPAHEKLQPRRSHSFSSSLRQKRDLLPDSYSCSEETEEK 0
0 VSLSSSYLEKVLGRSAFPSSPVALVTSSLRAASLPVGLNSSSASRGAGSDISQMKTEESHNNGGLDSIVSNTVPQIIIIPTSETNLFQEEPEEEETELFHFHDKKNNLLDLEGLSSSTEFLEAVEKFLS* 0

>MEL2_taeGut Taeniopygia guttata weak last exon full
0 MSTQPHTVTKAEIPGHILYTVGTCVLIIGSIGIIGNLLVLYAFYS 2
1 NKKLRTPQNYFIMNLAVSDFLMSASQAPICFVNSLHREWILGDI 1
2 GCNLYAFCGALFGITSMMTLLAISVDRYLVITKPLQSIQWTSKRRTVQIIALVWLYSLGW 1
2 SVAPLLGWSSYVPEGLMISCTWDYVTYSPANRSYTMILCCCVFFIPLVIIFHCYLSMFLAIRRTGR 2
1 DVQNLGSCSRKSYVSQSIKNEWKLAKIAFVVIIVFVLSWSPYACVTLIAWAG 2
1 RGNSLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2
1 KTIHQAVPCLRFLIRISKNDLLRGSINESSFRTSLCSHHSLAGKTKSICVSSISTGEA 0
0 TWSNVELDPVEPAQEKLKPRRSNSFSTSLRQEKRDLLPKTCSYDAATAQK 0
0 VSLSSSCLEKVLGQPVLPSPSAGLITSSLRATSLPVRLNSSRATTGDSSISHMATQESP RIIIIST 
QNHLFRKNWKRRD QKGNLLDLEGLSSSMEFFEAVEKFLS* 0

>MEL2_anoCar Anolis carolinensis (lizard) Gq +GRID2 +SMARCAD1 -ATOH1 +PDLIM5 +BMPR1B   
0 MGPHHRTKVDVPDHVLYTVGSCVLVIGCIGITGNLLVLYAFYS 2
1 NKRLRTPPNYFIMNLAVSDFLMSATQAPICFLNSMHKEWVLGDI 1
2 GCNLYAFCGALFGITSMITLLAISVDRYCVITKPLQSIKRTSKKRTCIIIVFVWLYSLGWSVCPLFGW 1
2 SSYIPEGLMISCTWDYVTYSPANRSYTMMLCCCVFFIPLVIIFHCYIFMFLAIRSTGR 2
1 RKSSISHSIKSEWKLAKIAFVAIVVFVLSWSPYACVTLISWAG 2
1 YARTLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2
1 RTIRSAVPCLRFLIPISKSDLSTSSMSESSFRASVSSRHSFSYRNKSTYISSISAKET 0
0 TWCDVELDPVESGHKKLQAYRSNSFSAKGVAEEESGLLLRTNNCNVPARKK 0
0 VALSSISLEESHAQSFGGNSSELLLLSSSLKTSSLPFGLNGSSTGESTDTSQLEGQENQINGSLDSFTSPILPQIIIIPT
SETNLSEGQAEPENAQDENIDLFFSQGKNHLLDIGRLSSSTELLEAIEKLLS* 0

>MEL2_podSic Podarcis sicula (wall lizard) 16688437 Cterm not full
0 MGTQHRIKVDVPDRVLYTVGSCVLVIGSIGITGNLLVLYAFYS 2
1 NKRLRTPANYFIMNLAASDFLMSATQAPICFLNSMHTEWILGDI 1
2 GCNFYVFCGALFGITSMMTLLAISVDRYCVITKPLQSIKRSSKKRSCIIIAFVWLYSLGWSVCPLFGW 1
2 SSYIPEGLMISCTWDYVSYSPANRSYTMMLCCFVFFIPLIIIFHCYLFMFLAIRSTGR 2
1 NVQKLGSTYNRKSNVSQSVKSEWKLAKIAFVAIVVFVLSWSP 2
1 YACVTLIAWAGYAKTLNPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2
1 RTIRSAVPCLRFLIRISPSDLSTSSVNESSFRASMSSRHSFAARNKSSCVSSISAAET 0
0 TWSDMELEPVEAARKKQQPHRSRSFSKQAEEETGLLLKTQSCNVLTGEK 0
0 VAVSSISLHDPFERSFGENAPELLLRPSCLRTSSLPFGLNSSSTEENADTSDMEVQEQHQMEASSH 0

>MEL2_xenLae Xenopus laevis (frog) Gq 0.0.1.2.2.1.1.1.0.0 indel +SMARCAD1 +PDLIM5 +BMPR1B melanopsin Xmop 21 0 0 
0 MDLGKTVEYGTHRQDAIAQIDVPDQVLYTIGSFILIIGSVGIIGNMLVLYAFYR 2
1 NKKLRTAPNYFIINLAISDFLMSATQAPVCFLSSLHREWILGDI 1
2 GCNVYAFCGALFGITSMMTLLAISINRYIVITKPLQSIQWSSKKRTSQIIVLVWMYSLMWSLAPLLGW 1
2 SSYVPEGLRISCTWDYVTSTMSNRSYTMMLCCCVFFIPLIVISHCYLFMFLAIRSTGR 2
1 NVQKLGSYGRQSFLSQSMKNEWKMAKIAFVIIIVFVLSWSPYACVTLIAWAG 2
1 HGKSLTPYSKTVPAVIAKASAIYNPIIYGIIHPKYR 2
1 ETIHKTVPCLRFLIREPKKDIFESSVRGSIYGRQSASRKKNSFISTVSTAET 0
0 VSSHIWDNTPNGHWDRKSLSQTMSNLCSPLLQDPNSSHTLEQTLTWPDDPSPKEILLPSSLKSVTYPIGLESIVKDEHTNNSCVR
NHRVDKSGGLDWIINATLPRIVIIPTSESNISETKEEHDNNSEEKSKRTEEEEDFFNFHVDTSLLNLEGLNSSTDLYEVVERFLS* 0

>MEL2_danRer Danio rerio (zebrafish) Gq 0.0.1.2.2.1.1.1.0.0 indel - +FLJ39155 +PDLIM5 -  
0 MEPQRQIYKRLDVPDHVHYIIAFLILIIGTLGVSGNALVMFAFYR 2
1 NKKLRSLPNYFIMNLAVSDFLMAITQSPIFFINCLYKEWMFGEL 1
2 GCKIYAFCGALFGITSMINLLAISIDRYLVITKPLQTIQWNSKRRTGLAILCIWLYSLAWSLAPLIGW 1
2 GSYIPEGLMTSCTWDYVSPSPANKSYTMMLCCFVFFIPLSIILYCYLFMFLSVRQASR 2
1 QKSSFVKQQSMRSEWKLAKIAAVVIVVYVLSWAPYACVTLVAWAG 2
1 HQDVLTPYSKTLPAVLAKSSAIYNPFIYAIIHNKYR 2
1 RTLAEKVPGLSCLSRSQKDGLSSSTNSDASAQDSSVSRQSSVSKNRLHSTMVQ* 0

>MEL2_tetNig Tetraodon nigroviridis (pufferfish) Gq 0.0.1.2.2.1.1.1.0.0 indel - - - +BMPR1B 404 aa 000 nm no_ref genome melanopsin  
0 MEPKDTHITSSFFSKVDVPDHVHYIIAFFVFVIGILGITGNVLVIFAFYS 2
1 NKKLRSLPNYFIVNLAVSDLLMASTQSPIFFINLYKEWMFGET 1
2 ACKMYAFCGALFGITSMINLLAISVDRYVVITKPLQTIRRSSKRRTALAILMVWLYSLAWSLAPLVGW 1
2 GSYIPEGLMTSCTWDYVTYTLANRSYTMMLCCFVFFIPLAIILCCYLLMFLAIRKTSR 2
1 RKSTLIQQKSIRSEWKLAKIAFVVIVVYVLSWSPYACVTLISWAG 2
1 YGSTLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2
1 KTIRSAVPCLRFLIPISKSDLSTSSMSDSSFRSALSCRHSYRSRSTYISSISAKET 0
0 TWCDVELDPVESGHKKLQAYRSNSFSAKGVAEEESGLLLRTNNCNVPARKK 0

>MEL2_gasAcu Gasterosteus aculeatus (stickleback) Gq 0.0.1.2.2.1.1.1.0.0 indel KNTC2 +FLJ39155 +PDLIM5 +BMPR1B 353 aa 000 nm no_ref genome melanopsin  
0 MEPDNAHTQRSFINKVDVPDHAHYIVAVFVVVIGTLGITGNALVMLAVYS 2
1 NKKLRNLPNYFIMNLAVSDFLMAFTQSPIFFINCLYKEWAFGET 1
2 GCKIYAFCGALFGIASMINLLAISIDRYLVITKPLQAIHWGSKRRTTLAILLVWLYSLAWSLAPLVGW 1
2 GSYIPEGLMTSCTWDYVTYTLANRSYTMMLCCFVFFIPLGIILYCYLFMFLAIRKTSR 2
1 RKSTLIKQKSMKSEWKLAKIAFVVIVVYVLSWSPYACVTLISWAG 2
1 HADILSPYSKAVPAIIAKASAIYNPFIYAIIHNKYR 2
1 MTLAAKFPCLRFLSPTPRKDTSSSISESSYRDSVISRQSTASRTHFITACPDTVN 0

>MEL2_gadMor Gadus morhua (cod)
0 MDMDRGFYRKVDVPDHAHYVIAFFVLIIGVVGVTGNALVMYAFLC 1
2 NKKLRTPPNYFIMNLAVSDFLMAITQSPIFFINSLFKEWIFGET 1
2 GCRMYAFCGALFGITSMINLLAISLDRYIVITKPPQAIRWVSGRRTMVVILLVWLYSLAWSLAPLLGW 1
2 SSYIPEGLMTSCTWDYVTSTPANKGYTLMLCCFVFFIPLGIISYCYLCMFLAIRSAGR 2
1 EIERLGTQVRKSTLMQQQTIKTEWKLTKVAFVVIIVYVHSWSPYACVTLIAWAG 2
1 YGSHLSPYSKAVPAVIAKASAIYNPFIYAIIHSKYR 2
1 DTLAEHVPCLYFLRQPPRKVSMSRAQSECSFRDSMVSRQSSASKTKFHRVSSTSTADTQVWSDVELDPMNHEGQSLRTSHSLGVLGRSKEHRGPP
AQQNRQTRSSDTLEQATVADWRPPLTALRCDRNFLPQPTHPPYKMAAATPLQATTVDNVTPEHWNKHPNNNHKNHNNRHNGNNNNEEHEYSGKGGRHCQN
HPHHIDVKNSISNCKKTCEKDTFSKEPVPCNAADDVRFSPRSAHTIQHAMGTPFRYMPEGDIACQERLSTDRSQRGDPLPDSKSLNCTGDVPVSAQRCSF
PHETSRNLEESFMAL* 0

>MEL1_strPur Stronglyocentrotus purpuratus GLEAN3_22851 opsin4 no cdna losing introns, expressed in larval postoral arm first exons missing
1 WTKSLRTPPNMLIVNLAISDFGMVITNFPLMFASTIYNRWLFGDA 1
2 GCQFYAFCGALFGIMSIANMTAIALDR 2
1 YYVICWSLEAVRSVTHRRSMIIIIIVWCYAIFWSIPPFFGVGSYVLEGYGLGCTFDFMTKDLNHYLHV
SFLFASSFVVPVTIIIVCFTRIAITVRAHRHELNKMRTKLTEDKDKKHKSSIRRANKAKTEFQIAKVGFQVTIFYVLSWM
PYSIVAVIGQYFDSDLLTPLGTVVPVIFAKCSAIWNPIIYCLSHEKFNAALKEKLMGMCGIEIPSKHRSMGSQESSVTGR
RGMHRQNSSTLSESSVTSTVDQDAIELKDRKQGPATVKVQQEKVEGGTYRRNPGDVTFSKDAGVEVDEKRRGDQGQRDDR
VRPQGEGQMDQWSQPPPAPASASAPTPGVNDKEYLTKM* 0

>MEL2_strPur Stronglyocentrotus purpuratus cdna: S.droebachiensis DQ285097 retained intron? WLEKMKTTQILHKPVTFLRLKSSFEPRFKPRFKRRF tube feet
0 MPTTLMENSTPGWMADDSQMEETHPAFPLIGGYLLVVVLLGTAGNSLVIYTFLRFKKLHSPINLLIVNLSASDLLVATTG
TPLSMVSSFYGRWLFGTNACAFYGFVNYYCGCISLNSLAAISVFRYIIVVRGQAQNNKLSLRSSIYAILVIHLYTLIFST
PPLYGWNRFVLAGYHTSCDIDFHTKTPLFVSYICYMFFFLFFLPLGLISWSYFKIYQRVSKHSNSMRTSFTGVTKEINSDEKHA 2
1 NHRRTASTLFVTIVVFLFAWFPYCIVSLWVLIGDANSISKLSTTIPSLFAKSSVIYNPLIYVVLNSKFRKALIQTLSFLKCLSKHELSESS* 0

See also: Curated Sequences | LWS | Encephalopsins | Neuropsins | Peropsins | RGR phyloSNPs | Update Blog

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