DHFR dihydrofolate

Alignment of vertebrate DHFR

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DHFR_homSap   MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_panTro   MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELASKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_gorGor   MVGSLNCIVAVSQNMGIGKNGVLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_ponAbe   MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_nomLeu   MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNYPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEFPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_macMul   MVSSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_papAnu   MARSLNCIVAVSQNMGIGKNGDLPWPPLR--SEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGNSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_calJac   MVQLLNCIVAVSQNMGIGKNGELPWPPLR--NEFRYFQRMTTTSSVEDKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGA-LLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPQYPGVPSDDQEEKGIKYKFEVYEKND---
DHFR_saiBol   MVQLLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSKELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_tarSyr   MVRPLYCIVAVSQNMGIGKDGTLPWPPLR--NEFRYFQRMTTMSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKVPPPGAHFLAKSLDDALKLIEQPELANKVDKVWIVGGNSVYREAMSQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_otogar   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND---
DHF1_micMur   MVRTLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_tupBel   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_musMus   MVRPLNCIVAVSQDMGIGKNGDLPWPPLR--NEWKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD---
DHFR_ratNor   MVRPLNCIVAVSQNMGIGKNGDLPWPLLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEIQEEKGIKYKFEVYEKKD---
DHFR_criGri   MVRPLNCIVAVSQNMGIGKNGDFPWPMLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELADKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFETDTFFPEIDLEKYKLLPEYPRVLPEVQEEKGIKYKFEVYEKKG---
DHFR_perMan   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA---
DHFR_perPol   MVRPLNCIVAVSQNMGIGKNGDLPWPLLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWVVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA---
DHFR_dipOrd   MGRSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELNNKVDMVWIVGGSSVYKEAMNQPGHHKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKID---
DHFR_speTri   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMMTTPSVEGKQNLVIIGRKTWFSIPEKNRPLNDRINIVLSRELKELPKGAHFLAKSLTDALKLTEQPELASKIDMIWIVGGSSVYKEAMIQPGHIKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILSEVQEEKGIKYKFEVYEKTD---
DHFR_cavPor   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFGYFQRMTTTPSVEGKQNLVIMGRKTWFSLPEKSRPLKDRINVVLSRELKEPPQGAHFLAPSLDDALKLIEQPGLANEVDMVWIVGGSSVYKEAMERPGHIKLFVTRIMQEFESDTFFPEIDLKKYRLLPEYPGVPSEIQEEKGIKYKFEVYEKND---
DHFR_oryCun   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLADALKLIEEPELAEKVDMAWIVGGSSVHKEAMNQPGHLRLFVTRIIQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_ochPri   MVRPLNCIVAVSQNMGIGRNGDLPWPPLR--NEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPPGAHFLAKSLDDALKLIEEPELAERVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILPEVQEERGIKYRFEVYEKND---
DHFR_felCat   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALELTEQPELAHKVDMVWVVGGSSVYKEAMNRPGHVRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDIQEEKSIKYKFEVYEKNN---
DHFR_canFam   MVRTLNCIVAVSQNMGIGRNGDLPWPPLR--NEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_vulVul   MVRTLNCIVAVSQNMGIGRNGTVPWPPLR--NEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_musPut   MVRTLNCIAAVSQNMGIGKNGDLPWPPLR--NEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_ailMel   MVRTLNCIAAVSQNMGIGKNGDLPWPPLR--NEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDIVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLDKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKND---
DHFR_equCab   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND---
DHFR_susScr   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEYKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLTEQPELKNKVDMVWIVGGSSVYKEAMNKPGHIRLFVTRIMKEFESDTFFPEIDLEKYKLLSECSGVPSDVQEEKGIKYKFEVYEKNN---
DHFR_turTru   MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEYRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDGALKLIEQPELTNTVDMVWIVGGGSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLIPEYPGVPSDVQEEKGIKYKFEVYEKNN---
DHFR_oviAri   MVRPLNCIVAVSQNMGIGKNGNLPWPPLR--NEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN---
DHFR_capHir   MVRPLNCIVAVSQNMGIGKNGNLPWPPLR--NEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKNN---
DHFR_bosTau   MVRPLNCIVAVSQNMGIGKNGNLPWPPLR--NEFQYFQRMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDVVWIVGGSSVYKEAMNKPGHVRLFVTRIMQEFESDAFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN---
DHFR_myoLuc   MVRTLNCIAAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNQVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE---
DHFR_pteVam   MVLSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_sorAra   MVRPLNCIVAVSQNMGIGKNGELPWPPLR--NEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD---
DHFR_loxAfr   MVRPLNCIVAVSQNMGIGKNGDLP-PPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRGLKEPPQGAHFLAKSLDDALKLIEQPELRNKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKRLSEYPGVLSDVQEEKGIKYKFEVYEKKD---
DHFR_proCap   MVRPLSCIVAVSQNMGIGKNGDLPWPPLR--NEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD---
DHFR_monDom   MVRQLNCIAAVSKNMGIGKDGDLPWPLLR--NEFKYFQKMTTTPSVEGKQNLVIMGKKTWFSIPEKCRPLKDRINVVLSRELKEPPQGAHYLASSLDNALNLLELPELADKVDMVWIVGGSSVYKEAMNKPGHQRLFITRILQEFESDTFFPEIDLKKYKQLQDYPGVSPDVQEEKGIQYKFEVYEKHE---
DHFR_macEug   MAPTINCIVAVAQNMGMGKNGDLPWPPLR--KEFQYFQKMTTTPSVEGKQNLLIMGKKTWFSIPEKNRPLKDRINLVLSRELKDPPLGAHYLAKSLDDALDLLRLPELANTVDMVWVVGGSSVYKEAMNKPGHQRLFVTRILQEFESDTFFPEIDLKRYKLLPEYPGVLTDVQEEKGIQHKCEIHEKHD---
DHFR_sarHar   MVRPINCIAAVSQNMGIGKNGDLPWPPLR--NEFNYFQKMTTTSSVEGKQNLLIMGKKTWFSIPEKHRPLKDRINLVLSRELKEPPPGAHYLAKSLDDALDLLELPELADKVDMIWVIGGSSVYKEAMEKPGHQRLFVTRILQEFECDTFFPEIDLKKYKLLPEYPGVLTDIQEEKGIQYKFEVYEKHD---
DHFR_triVul   MVRTINCIVAVSQNMGIGKNGDLPWPPLR--NDFKHFQKMTTIPSVEGKQNLLITGKKTWFSIPEKSRPLKDRINVVLSRELKEPPPGAHYLAKSLDDALDLLELPELANKVDMIWVVGGSSVYKEAMNKRGHNRLFVTRILQEFESDTFFPEIDLKRYKLLXEYPGVLTDVQEEKGIQYKFEVYEKHD---
DHFR_ornAna   MGPLLNCIVAVAKNGGIGNKGDLPWPALR--NEFRYFQKMTTTPTVKGKQNLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLHLPELASKVDMVWIIGGSSVYKEAMEKGGHQRLFVTRILQDFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI---
DHFR_tacAcu   MGRPLNCIAAVAKNGGIGNKGDLPWPPLR--NEYRYFQKMTTTPTVEGKENLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLNLPELASKVDMVWIIGGSSVYKEAMEKPGHQRLFVTRILQEFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI---
DHFR_galGal   MVRSLNSIVAVCQNMGIGKDGNLPWPPLR--NEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEENGIQYKFEVYQKSVLAQ
DHFR_lagLag   MVRSLNSIVAVCQNMGIGKDGNLPWPPLR--NEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYQKSVLAQ
DHFR_anaPla   MVRSLNSIVAVCQNMGIGKDGNLPWPPLR--NEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYEKTVLAQ
DHFR_taegut   MPRSLNSIVAISQNMGIGKDGRLPWPPLR--NDYKYFQRMTSASPVEGKKNAVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELQSKVCMVWIVGGSSVYKEAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKTVVP-
DHFR_ficHyp   MVRSLNSIVAVSQNMGIGKDGRLPWPPLR--NEYKYFQRMTSTPRVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAVV--
DHFR_melUnd   MVRSLNSIVAVCQNMGIGKDGSLPWPPFR--NEYKYFQRMTSTSQVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELQETPKGAHYLSKNLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILQEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAV---
DHFR_allMis   MVSSLNAIAAVSQNMGIGKNGTLPWPPLR--NEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV---
DHFR_croPor   MVSSLNAIAAVSQNMGIGKNGTLPWPPLR--NEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV---
DHFR_chrPic   MVRPLNCIAAVCQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTTTVEGKQNVLIMGKRTWFSIPEKNRPLKDRINIVLSRELKETPKGAHYLAKSLDDALVLLDSPELKSKIDMVWIIGGSSIYKAAMEKPVHQRLFVTRILHEFESDTFFPEIDLEKYKHLPEYPGVPADVQEENGIRYKYEVYEKTV---
DHFR_anoCar   MVLSLNSIAAVCQNMGIGKNGQLPWPPLR--NEFKYFQKMTMTPTQEGKQNVVIMGKRTWFSIPEKNRPLKDRINIILSREMKEVPKGAHYLARSLDEALDLLQSPELENKIDLVWIVGGSSVYKAAMDKPIHHRLFVTRILHDFESDTFFPEIDLEKYKLIPSYAGVPTDIQEENGIRYRFEVYEKNI---
DHFR_pytMol   MVASLHSIVAVCNNMGIGKDGKLPWPPLR--NEFKHFQKMTMTTKEEGKQNVVIMGKKTWFSIPEKHRPLKNRINVVLSKELKDVPEGAHYLAKSLEEALDLLETSEMERKVDKVWIVGGSSVYKAAMEKPIHHQLFVTRIMHDFESDTFFPEIDLKKYKLLPKYTGIPTDIQEENGIQYKFEVYENTI---
DHFR_ambMex   MGRILNAIVAVCPSMGIGKDGNLPWPILR--NEFKYFQRMTMTATQEGKQNVVIMGRKTWLSIPEKNRPLKGRINIVLSRELTAPPPGAHYLANSLDGALELLETRELAGKVDLLWIIGGSSLYRELIGRPHHQRLFVTRILKEFECDTFLPEFDLEKYRLLPEYPGVSLGIQEENGIQYKFEVYEKVEEA-
DHFR_xenTro   MNPFLHAVVAVCPNQGIGKEGSLPWPLLR--NEFKHFQRLTMTPTVEDKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLAEAIDLLEDPELKDKVDLVWVIGGSSLYQELMEKPVNQRWFVTRILQEFECDTYLPEIDLNSFRLLPEYPGISPELQEENGVQYKFEVYEKIV---
DHFR_xenLae   MNQFLHAVVAVCPNQGIGKGGSLPWPLLR--NEFKHFQRLTMTPTVEGKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLTEAIDLLEDPELKDKVDLVWIIGGSSLYQELMEKPINQRWFVTRILQEFECDTYLPEINLNSFQLLPQYPGISPELQEENGVQYKFEVYEKIV---
DHFR_ranCat   MNQFLHAVVAVCPNQGIGKGGTLPWPLLR--NEFKHFQRLTMTPTTEGKQNVVIMGRRTWFSIPEKNRPLKGRINIVLSRELTEPPNGAHYLAKSLDEAVHLLKTPEMKEKIDLIWIIGGSSLYKEVMDKPVSQRLFITRIQQEFECDTFLPDVDLQSYKLLPEYPGISSEMQEENGIQYKFEVYEKML---
DHFR_latCha   MARLLNSIVAVCPNLGIGKDGNLPWHPKRLSNEFRYFQKMTTTPTVEGKQNVVIMGRKTWFSIPEKNRPLKGRVNVVLSRELQEAPEGAHYLARDLDSALKLLESPELADRVDLVWIIGGSALYKEALEHPSQHRLFVTRVLQQFECDTYLPEINFNKFTLLPEFPGVSSAIQEESGIQYKFEVYEKNS---
DHFR_gadMor   MSRVLNCIVAVCPDAGIGYKGDLPWHPTRLNNEFKHFRRLTVTPGAEDKQNVVIMGRKTWNSIPEKNRPLNDRINIVLSRQLEAPPSGAHHLARDLPSALRLLDVPE-GLLADQVWLIGGSSMYSEVMETPCPRRLFVTRILKQFPSDTFFPDISPAQFRLLPEYPGVSSELQEENGIQYRFQVYQSVDP--
DHFR_tetNig   MARVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTWFSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVET-QLAEQADQVWVIGGSSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQYRYQVYESLEP--
DHFR_hipHip   MSRILNGIVAVCPDLGIGNRGNLPWHPVRLSNEFKHFRSMTATPSEKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKAPPAGAHHLSADFSSALRLVDT-ELADQADQVWVIGGSSLYKEMMESPGTRRLFVTRILKQFDCDTFLPEISPDKYRQLPEFPGVPQELREENGIQYRFEVYESIEP--
DHFR_solSen   MSRVLNGIVAVCPDMGIGMTGNLPWHPVRLSNEFKHFRTMTATSSVKGKKNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKLPPAGAHHLAADFSSALRLVET-ELADEADQIWIIGGSSLYKEMMESPGIKRLFVTRILKQFECDTFLPEISLDKYRLLPEFAGVSQELQEENAIQYRFEVYESVEQ--
DHFR_oreNil   MPRVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTWYSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDT-ELADQADQVWVIGGSSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQYRFKVYESIQE--
DHFR_dicLab   MSRILNGIVAVCPDLGIGMNGNLPWHPVRLGNEFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSKECKVPPAGAHHLAADFNSALRLVET-ELADQADQVWVIGGSSLYKELMESPGTRRLFVTRILKQFECDTFLPEICPDKYQLLPEFPGVSQELQEENGIQYRFEVYESTEQ--
DHFR_perFla   MSRILNGIVAVCPDLGIGNNGNLPWHPVRLNNEFKHFRTMTATPSVNGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRECKAPPAGAHHLARDFSSALRLVDT-ELAQQADTVWVIGGSSLYKEMMESPGTRRLFVTRILKQFECDTFLPEITPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIQQ--
DHFR_spaAur   MSRIVNGIVAVCPDLGIGNNGNLPWHPVRLNREFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPDKFRPLNNRINIVLSRECKVPPAGAHHLAADFSSALSLVDT-ELADQADQVWVIGGSSLYKELMESTRTSRLFVTRILKQFECDTFLPEISPDKYSLLPEFPGVPQGLQEENGIQYKFEVYERIKQ--
DHFR_gasAcu   MSRVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDT-ELAEQADQVWVIGGSSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQYRFEVYESIDV--
DHFR_oryLat   MTRTLNGIVAVCPDLGIGKGGNLPWHPLRLSKDFALFRKMTSTPLVAGRQNVVIMGRKTWFSIPEKNRPLQNRINIVLSRQLKAPPAGAHFVAADFAAALRLVDS-ELAERADQVWIIGGSSIYXEMMESPAPRRLIVTRVQKQFECDTFFPEIRPEQFRLLPEFPGVSQELQEENGVSYKFEVYESIQQ--
DHFR_anoFim   MSRVLNAIVAVCPDLGIGRNGDLPWHPVRLNNEFKHFRRMTSTPSVEGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKTPPAGAHHLAQDFNSALRLVDM-KLAEQVDQVWVIGGSSLYKELMESQGIRRLFVTQILKQFECDTFLPKISPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIEQ--
DHFR_esoLuc   MSRVLNCIVAVCPNMGIGNKGNLPWHPKRLNNEFKYFQKMTMTPFVEGKQNVDIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPPGAHHLASDFSSALRLLDTAEMAERVDQVWVIGGSSLYKEMMESEGIRRLFVTRILQQFESDTFLPEINQDKYRLLPEFPGVPQDVQEESGIQYRFEVYESVDH--
DHFR_salSal   MSRVPNCIVAVCPDVGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNVVIMGRKTWFSIPERNRPLKNRINIVLSRELKKPPAGAHHLASDFRSALCLLDMAEMAERADQVWVIGGSSLYKEMMESQGTRRLFVTRILQQFKSDTFLPEINQDQYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ--
DHFR_oncMyk   MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ--
DHFR_danRer   MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH--
DHFR_cteIde   MSRILNCIVAVCPDMGIGRKGNLPWHPIRLSNEFKHFQKMTMTPSVEGKKNVVIMGRKTWFSIPAQNRPLKNRINIVLSRELKTAPEGAHYLASDLSSALHLLDSSELEKQVDQVWIIGGSALYKEVMERSGPRRLFVTRVLKQFDCDTFIPEINMDKYKLLPEFPGVPTGLQDENGVQYVFECMIVS----
DHFR_cypCar   MSRILNSIVAVCPDMGIGKNGNLPWHPIRLSNEFKHFQKMTMTPLVEGKKNVVLMGRKTWFSIPAANRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSSELEKQVDQVWIIGGSALYKEVMESSGPRRLFVTRVLKQFDCDTFIPDINMDKYKLLPEFPGVPTGLQEENGVQYVFEVYESTDH--
DHFR_ictPun   MGRVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYESVEQ--
DHFR_leuEri   MTRLINSIVAVCPNMGIGNNGNFPWHPIRLSKEFKHFQRMTSTPSVEGKCNAVIMGRKTWHSIPEKNRPLKNRINIVLSRELKEVPEGAHYLAPDLDSALALLDSSDLQDKVDLVWIIGGSALYKEALESPASHRLFVTRILQDFDSDTFIPEINLNKFKLLPEFPDVPADIQEENGIRFKYEVYERISPV-
DHFR_squAca   MPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA-
DHFR_eptBur   MQNPVNVIAAVLPNMGIGWKGGLPWHSKSLVKEMKHFTRLTSAAA-EGKQNAVVMGRKTWESIPEKFRPLRNRINVVLSRTLSHLPLGAHYLATDFSNALAQLSESELCCRIDHIWIIGGSSIYKEALLHPACHRIFITRIMHDFEADTFFPHIDSKRFSLCQSYPGIPEEIQEENGVQYKFEVYEVVAK--
DHFR_petMar   -----------------------------------------------GKQNVVIMGRKTWFSIPEKKRPLKNRINIVLSRELKCLPEGAHYLVPDLNSALSLIDSPAIQEKLDGVWILGGGGVYKEAMEHSACRRLFITRVLQTMEADAFFPDIDADKFKLLP-----------------------------

                      10        20          30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190
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DHFR_homSap   MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND---
DHFR_panTro   .............................................................................................................S..................................................................................
DHFR_gorGor   .....................V..........................................................................................................................................................................
DHFR_ponAbe   .......................................................................D........................................................................................................................
DHFR_nomLeu   .................................................................................................................................Y..................................F...........................
DHFR_macMul   ..S.........................................................................................A.....................I...................................L.........................................
DHFR_papAnu   .AR............................S............................................................A.....................I.....N.............................L.........................................
DHFR_calJac   ..QL.................E.........................D........R..............D...V.............-L.A.....................I..I..........D..................................Q....P..D....................
DHFR_saiBol   ..QL....................................................R..............D...V...K............A.....................I..I..........D...........................K.....S.............................
DHFR_tarSyr   ..RP.Y.............D.T....................M.............R..............D...........V..P.....AK........I..........K......N...R...SQ................................S.............................
DHFR_otogar   ..RP..............................N......K..............R..............D...I........S.......AK........I.E........................Q...V............C.........K.....S.............................
DHF1_micMur   ..RT..................................K.................R.........S....D...V...............VAK........I..................A.......Q..........................K...................................
DHFR_tupBel   ..RP..............................K................I....R..............D....................AK........I.......Q.............R....Q..............................................................
DHFR_musMus   ..RP.........D...................WK.....................R..............D...I..........R.....AK......R.I......S..............Q....Q....R........E............G.............E................K....
DHFR_ratNor   ..RP......................L.......K.....................R..............D...I................AK........I......S......V.......Q....Q....R........E..........................EI...............K....
DHFR_criGri   ..RP..................F...M.......K.....................R..............D...I................AK........I......D...................Q....R........E..T...................R..PE................KG...
DHFR_perMan   ..RP..............................K.....................R..............D...I................AK........I..........................K....R........E........D.................E....E...........KA...
DHFR_perPol   ..RP......................L.......K.....................R..............D...I................AK........I.............V............K....R........E........D.................E....E...........KA...
DHFR_dipOrd   .GR...............................K.........T...........R..............D...I................AK...E....I.....N....................Q...H.........E..........................E................I....
DHFR_speTri   ..RP..............................K.....M..P..........I.R.............ND...I........L.K.....AK..T............S.I..I.............IQ...I.........E.......................I..E................T....
DHFR_cavPor   ..RP..............................G........P............R.....L...S....D...V................AP........I...G...E.................ER...I.........E............K..R........P.EI....................
DHFR_oryCun   ..RP....................................................R..............D...V................AK..A.....I.E....E....A........H.....Q....R......I.E................................................
DHFR_ochPri   ..RP..............R...............K.........T...........R..............D...I..........P.....AK........I.E....ER.................DK....R........E.......................I.PE....R....R...........
DHFR_felCat   ..RP..............................K.....................R..............D...I....D...........AK......E........H......V............R...VR........E...........................I....S...........N...
DHFR_canFam   ..RT..............R...............K.......N.............R..............D...I....D...........AK...................................K....R.......HE................................................
DHFR_vulVul   ..RT..............R..TV...........K.......N.............R..............D...I....D...........AK...................................K....R.......HE................................................
DHFR_musPut   ..RT....A.........................KF....................R..............D........D...........AK........I..........................K....R........E................................................
DHFR_ailMel   ..RT....A.........................KF....................R..............D........D...........AK........I..........I...............K....R........E............D...........P.......................
DHFR_equCab   ..RP..............................K.......A.............R..............D....................AK......S.L..........................K....R........E...........F...............I....................
DHFR_susScr   ..RP.............................YK.....................R..............D...I................AK..............K....................K...IR.......KE..................S.CS..P...................N...
DHFR_turTru   ..RP.............................Y......................R..............D...I................AK...G....I.....T.T.........G........K....R........E.................I......P...................N...
DHFR_oviAri   ..RP.................N............K.......V..E..........R..............D...I..........K.....AK......E.I.D...T....................K....R........E....V......F............PL..................N...
DHFR_capHir   ..RP.................N............K.......V..E..........R..............D...I..........K.....AK......E.I.D...T....................K....R........E....V......F............P...................N...
DHFR_bosTau   ..RP.................N............Q.......V.............R..............D...I..........K.....AK......E.I.D...T....V...............K...VR........E....A......F............PL..................N...
DHFR_myoLuc   ..RT....A.........................K.....................R.......K......D..............K.....AK........I.E...T.Q.................SK...QR........E............................................E...
DHFR_pteVam   ..L...............................K..............R......R.......K......D..............K.....AK........I.....RS...................K...I.....K...E....I.......G...................................
DHFR_sorAra   ..RP.................E............T..RK.................R..............D..............T.....AK........I.....T...............Q.V..K....R........E..C...............TD....V..................K....
DHFR_loxAfr   ..RP....................-.........K.....................R..............D........G...........AK........I.....R....................Q....R........E................R.S........................K....
DHFR_proCap   ..RP.S............................KH.RT..S.P............R..............D....................AK........I.....T....L...I..........SQ....T........E..............F.........P..................K....
DHFR_monDom   ..RQ....A...K......D......L.......K...K....P......................C....D...V..............Y.AS...N..N.L.L....D...................K...QR..I...L.E............K...Q.QD....SP........Q........HE...
DHFR_macEug   .APTI......A....M..............K..Q...K....P........L..................D...........D..L...Y.AK......D.LRL.....T.....V............K...QR......L.E............KR...........T........QH.C.IH..H....
DHFR_sarHar   ..RPI...A.........................N...K.............L.............H....D..............P...Y.AK......D.L.L....D....I.VI..........EK...QR......L.E..C.........K............T.I......Q........H....
DHFR_triVul   ..RTI...........................D.KH..K...IP........L.T...........S....D...V..........P...Y.AK......D.L.L.........I.V............KR..NR......L.E............KR....X......T........Q........H....
DHFR_ornAna   .GPL.......AK.G...NK......A...........K....PT.K...................S....D...I..............YVAK......N.LHL....S.......I..........EKG..QR......L...................I......PA.T......Q.R......HI...
DHFR_tacAcu   .GRP....A..AK.G...NK.............Y....K....PT....E................S....D...I..............YVAK......N.LNL....S.......I..........EK...QR......L.E.................I......PA.T......Q.R......HI...
DHFR_galGal   ..R...S....C.......D.N...........YK......S..H......A...................D...I........A.K...Y..K......A.LDS...KS..........TA...A..EK.INHR......LHE...........YKDF...T.....PA.I...N..Q......Q.SVLAQ
DHFR_lagLag   ..R...S....C.......D.N...........YK......S..H......AL..................D...I........A.E...Y..K......A.LDS...KS..........T....A..EK.INHR......LHE...........YKDF...T.....PA.....N..Q......Q.SVLAQ
DHFR_anaPla   ..R...S....C.......D.N...........YK......S..H......AL..................D...I........A.E...Y..K......A.LDS...KS..........T....A..EK.INHR......LHE...........YKDF...T.....PA.....N..Q........TVLAQ
DHFR_taegut   .PR...S...I........D.R..........DYK......SA.P....K.A....R..............D...I........A.K...Y..K......A.LDS...QS..C...............EK.I.HR......LKE...........HKD....T.....PA.I...N..Q........TVVP.
DHFR_ficHyp   ..R...S............D.R...........YK......S.PR......AL..................D...I........A.E...Y..K......A.LDS...KS..........T....A..EK.I.HR......LKE...........HKD....T.....PA.I...N..Q........AVV..
DHFR_melUnd   ..R...S....C.......D.S.....F.....YK......S..Q......AL..................D...I......Q.T.K...Y..KN.....A.LDS...KS..........T....A..EK.INHR......L.E...........HKD....T.....PA.I...N..Q........AV...
DHFR_allMis   ..S...A.A............T............K........P..P....VL..................D...I......A.V.K...H.A.......V.LDS...K..I.L...........A..EK.INQR......LHE...............R....F...PA.....N..H........TV...
DHFR_croPor   ..S...A.A............T............K........P..P....VL..................D...I......A.V.K...H.A.......V.LDS...K..I.L...........A..EK.INQR......LHE...............R....F...PA.....N..H........TV...
DHFR_chrPic   ..RP....A..C......................K........TT......VL....R.............D...I........T.K...Y.AK......V.LDS...KS.I.....I....I..A..EK.V.QR......LHE................H.......PA.....N..R..Y.....TV...
DHFR_anoCar   ..L...S.A..C.........Q............K...K..M.PTQ.....V.....R.............D...II....M..V.K...Y.A....E..D.LQS...E..I.L...........A..DK.I.HR......LH..................I.S.A..PT.I...N..R.R.......I...
DHFR_pytMol   ..A..HS....CN......D.K............KH..K..M.TKE.....V..............H....N...V...K...DV.E...Y.AK..EE..D.L.TS.MER...K...........A..EK.I.HQ.......H.............K......K.T.IPT.I...N..Q.......NTI...
DHFR_ambMex   .GRI..A....CPS.....D.N....I.......K......M.ATQ.....V....R...L..............I......TA..P...Y.AN...G..E.L.TR...G...LL..I....L.R.LIGR.H.QR......LKE..C...L..F.....R........SLGI...N..Q........VEEA.
DHFR_xenTro   .NPF.HAV...CP.Q....E.S....L.......KH...L.M.PT..D.K.V....R..............E...I...K......T...Y..K..AE.ID.L.D...KD...L..VI....L.Q.L.EK.VNQRW.....L.E..C..YL.....NSFR.......ISPEL...N.VQ........IV...
DHFR_xenLae   .NQF.HAV...CP.Q....G.S....L.......KH...L.M.PT....K.V....R..............E...I...K......T...Y..K..TE.ID.L.D...KD...L...I....L.Q.L.EK.INQRW.....L.E..C..YL...N.NSFQ...Q...ISPEL...N.VQ........IV...
DHFR_ranCat   .NQF.HAV...CP.Q....G.T....L.......KH...L.M.PTT.....V....RR.................I......T...N...Y.AK...E.VH.LKT..MKE.I.LI..I....L...V.DK.VSQR..I...Q.E..C...L.DV..QS.........IS.EM...N..Q........ML...
DHFR_latCha   .ARL..S....CP.L....D.N...H.K.LS.......K....PT......V....R................V.V......Q.A.E...Y.A.D..S....L.S....DR..L...I...AL....LE..SQHR.....VL.Q..C..YL...NFN.FT....F...S.AI...S..Q.........S...
DHFR_gadMor   .SRV.......CPDA...YK.....H.T.LN...KH.R.L.V.PGA.D...V....R...N.........ND...I....Q.EA..S...H.A.D.PS..R.LDV..-GLLA.Q..LI....M.S.V.ET.CPRR......LKQ.P......D.SPAQFR........S.EL...N..Q.R.Q..QSV.P..
DHFR_tetNig   .ARV..A....CPDL...R......H.I.LD...KH.RK..S.P..N....V....R.........H...AN...I....RSR...A...H.AHD.SS..Q.V.T-Q..EQA.Q..VI....L.Q.M.ER..TSR....HVLKQ.DC...L...RPQR.R...QF...PQEL......Q.RYQ...SLEP..
DHFR_hipHip   .SRI..G....CPDL...NR.N...H.V.LS...KH.RS..A.P.EK....V....R.............NN...I....QC.A..A...H..ADFSS..R.VDT-...DQA.Q..VI....L...M.ES..TRR......LKQ.DC...L...SPD..RQ...F...PQELR..N..Q.R.....SIEP..
DHFR_solSen   .SRV..G....CPD....MT.N...H.V.LS...KH.RT..A....K..K.V....R.............NN...I....QC.L..A...H.AADFSS..R.V.T-...DEA.QI..I....L...M.ES..IKR......LKQ..C...L...S.D..R....FA..SQEL...NA.Q.R.....SVEQ..
DHFR_oreNil   .PRV..A....CPDR...NK.N...H.I.LSK..AH.RK..A.P..K....V........Y.........SN...I.....C.V..A...Y.ASDFSS..R.IDT-...DQA.Q..VI....L...M.GST.MRR....Q.LKQ..C...L...S.D..R....F.D.PQEL...N..Q.R.K...SIQE..
DHFR_dicLab   .SRI..G....CPDL...M..N...H.V.LG...KH.RT..A.P..K....V....R.............NN...I...K.C.V..A...H.AADFNS..R.V.T-...DQA.Q..VI....L...L.ES..TRR......LKQ..C...L...CPD..Q....F...SQEL...N..Q.R.....STEQ..
DHFR_perFla   .SRI..G....CPDL...N..N...H.V.LN...KH.RT..A.P..N....V....R.............NN...I.....C.A..A...H.A.DFSS..R.VDT-...QQA.T..VI....L...M.ES..TRR......LKQ..C...L...TPD..R....F...PQEL...N..Q.R.....SIQQ..
DHFR_spaAur   .SRIV.G....CPDL...N..N...H.V.LNR..KH.RT..A.P..K....V....R.......D.F...NN...I.....C.V..A...H.AADFSS..S.VDT-...DQA.Q..VI....L...L.ESTRTSR......LKQ..C...L...SPD..S....F...PQGL...N..Q.......RIKQ..
DHFR_gasAcu   .SRV..G....CPDL...CH.N...H...LS...KH.R...A.A..KD...V....R.............NN...I.......A..A...H.APDFSS..R.VDT-...EQA.Q..VI....L...LLES..TRR......LKQ..C...L...CPDR.R....F...PEEL...N.VQ.R.....SI.V..
DHFR_oryLat   .TRT..G....CPDL....G.N...H...LSKD.AL.RK..S.PL.A.R..V....R.............QN...I....Q..A..A....VAADFAA..R.VDS-...ERA.Q...I....I.X.M.ES.APRR.I...VQKQ..C.......RP.QFR....F...SQEL...N.VS.......SIQQ..
DHFR_anoFim   .SRV..A....CPDL...R......H.V.LN...KH.R...S.P.......V....R.............NN...I.......T..A...H.AQDFNS..R.VDM-K..EQ..Q..VI....L...L.ESQ.IRR....Q.LKQ..C...L.K.SPD..R....F...PQEL...N..Q.R.....SIEQ..
DHFR_esoLuc   .SRV.......CP.....NK.N...H.K.LN...K...K..M.PF......VD...R........R.....N...I..........P...H.ASDFSS..R.LDTA.M.ER..Q..VI....L...M.ESE.IRR......L.Q......L...NQD..R....F...PQ.....S..Q.R.....SV.H..
DHFR_salSal   .SRVP......CPDV...N..N...H.K.LN...K...K..M.........V....R........R.....N...I.......K..A...H.ASDFRS..C.LDMA.M.ERA.Q..VI....L...M.ESQ.TRR......L.Q.K....L...NQDQ.R....F...PQ.L...A.LQ.R.....SI.Q..
DHFR_oncMyk   .SRV.......CPD....N..N...H.K.LN...K...K..M.........A....R........R.....N...I..........A...Q.ASDFRS..R.LDTA.M.ER..Q..VI....L.E.M.ESQ.TRR......L.Q.K....L...NQD..R....F...PQ.L...A.LQ.R.....SI.Q..
DHFR_danRer   .SRI.......CPD.......N...H.I.LS..LKH..K..M.P.D...K.V....R.......AAH....N...I.......TA.E...Y.ASDFSS..H.LDSG..EKL..Q...I....L...V.ERS..RR......LKQ.DC...I.NF.MD.......F...PVGL..DN.VQ.L.....SIKH..
DHFR_cteIde   .SRI.......CPD....RK.N...H.I.LS...KH..K..M.P.....K.V....R.......AQ.....N...I.......TA.E...Y.ASD.SS..H.LDSS..EKQ..Q...I...AL...V.ERS.PRR.....VLKQ.DC...I...NMD.......F...PTGL.D.N.VQ.V..CMIVS-...
DHFR_cypCar   .SRI..S....CPD.......N...H.I.LS...KH..K..M.PL....K.V.L..R.......AA.....N...I.......TA.E...Y.ASDFSS..H.LDSS..EKQ..Q...I...AL...V.ESS.PRR.....VLKQ.DC...I.D.NMD.......F...PTGL...N.VQ.V.....ST.H..
DHFR_ictPun   .GRV.......CPD....R..N...H.I.LSK..KH..K..M.PT....K.V....R.......AQ.....N...V.......TA.E...Y.AADFSS..RIL.NA..EAQA.Q...I....L...V.ESSA.QR......LKQ.DC...I.N...D.FR....F...PAGL...N.LQ.V.....SVEQ..
DHFR_leuEri   .TRLI.S....CP.....N..NF..H.I.LSK..KH.....S.P.....C.A....R...H..........N...I........V.E...Y.APD..S..A.LDSSD.QD...L...I...AL....LES.ASHR......L...D....I...N.N.F.....F.D.PA.I...N..RF.Y....RISPV.
DHFR_squAca   .PRLV......CP......D.NF..H.I.LSK.LKH..K..A.P.....R.A....R.........H....D.L.I........L.H..NY.AHD..S..AHLDSS..QD...L...I...TL.....ES.VSHRI.....LK.......L..M..N.F....KF.D.PTE...DN..Q.......RIAHA.
DHFR_eptBur   .QNPV.V.A..LP.....WK.G...HSKSLVK.MKH.T.L.SAAA-.....A.V..R...E.....F...RN...V....T.SHL.L...Y.ATDFSN..AQLSES..CCRI.HI..I....I....LL..ACHRI.I....H...A.....H..SKRFS.CQS...IPEEI...N.VQ.......VVAK..
DHFR_petMar   -----------------------------..----------------....V....R.........K....N...I.......CL.E...Y.VPD.NS..S.IDS.AIQE.L.G...L..GG......E.SACRR..I..VL.TM.A.A...D..AD.F....--------------------------...

Vertebrate reference sequences

>DHFR_homSap Homo sapiens (human) AC130896 tightly syntentic -+ MSH3 chr5
0 MVGSLNCIVAVSQNMGIGKNGDLPWPPLR 2
1 NEFRYFQRMTTTSSVE 1
2 GKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELK 2
1 EPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYK 0
0 EAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE 2
1 YPGVLSDVQEEKGIKYKFEVYEKND* 0

>DHFR_panTro Pan troglodytes (chimp) XM_001138522
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELASKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_gorGor Gorilla gorilla (gorilla) CU104654
MVGSLNCIVAVSQNMGIGKNGVLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_ponAbe Pongo abelii (orangutan) XM_002815704 syntenic 
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_nomLeu Nomascus leucogenys (gibbon) XM_003261693
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNYPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEFPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_macMul Macaca mulatta (rhesus) XM_001110551 syntenic
MVSSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_papAnu Papio anubis (baboon) FC175564
MARSLNCIVAVSQNMGIGKNGDLPWPPLRSEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGNSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_calJac Callithrix jacchus (marmoset) XM_002745986 tightly syntentic -+ MSH3 4 recent pseudogenes
MVQLLNCIVAVSQNMGIGKNGELPWPPLRNEFRYFQRMTTTSSVEDKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGALLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPQYPGVPSDDQEEKGIKYKFEVYEKND 

>DHFR_saiBol Saimiri boliviensis (squirrel_monkey) AGCE01006205/AGCE01053749 numerous pseudogenes syntentic MSH3
MVQLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSKELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDlkkykllseYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_tarSyr Tarsius syrichta (tarsier) ABRT010238682/ABRT010238686/ABRT010468683
MVRPLYCIVAVSQNMGIGKDGTLPWPPLRNEFRYFQRMTTMSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKVPPPGAHFLAKSLDDALKLIEQPELANKVDKVWIVGGNSVYREAMSQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_otogar Otolemur garnettii (bushbaby) AAQR03040271/AAQR03040268
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND

>DHF1_micMur
MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPE YPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_tupBel Tupaia belangeri (treeshrew) AAPY01231248/AAPY01231253/AAPY01729648/SRA|SRR005484.246484.2
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE
 
>DHFRL1_homSap Homo sapiens (human) NM_001195643 synteny +ARL13B/-DHFRL1/+NSUN3 chr3
MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEGKHIKYKFEVCEKDD

>DHFRL1_panTro Pan troglodytes (chimp) ti|1986883368 syntenic short c-terminal
MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLNDRINLVLSRELKEPPQGAHFFARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEGKHIKYKFEVYEND

>DHFRL1_gorGor Gorilla gorilla (gorilla) ti|2051780408/ti|2033585870 10 bp deletion so frameshift synteny unknown but 97% identical homSap
MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAISLDDALKLTERPKLANKVDMIWIVGGSSVYKEAMNh lKLFVTRIMQDFASDTFFSEIDLEKYKLLPEYPGVLSDVQEgKHIKYKFEAYEKND
 
>DHFRL1_ponAbe Pongo abelii (orangutan) genome/blat syntenic 
MVRLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_nomLeu Nomascus leucogenys (gibbon) ADFV01005477 syntenic 
MVRLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLEDRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNRSGHLKLFVTRIMQDFESDMFFSEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_macMul Macaca mulatta (rhesus) genome/blat syntenic
MVRSLNCIVAVSQNMGIRKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_papHam Papio hamadryas (baboon) ti|1986883368 synteny unknown 
MVHSLNCIVAVSQNMGIGKNGDLPWPPLRNEFGYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFLTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKN

>DHFRL1_calJac Callithrix jacchus (marmoset) genome/blat frameshift and stop codon but syntenic
mvrllCIVAVSQNMGIGKKGDLPWPPLRNEFRYFQRMTTTSSIEGKQNLVIMGRKTWFSIPEKNRPLKDRINFVLSRELQEPPQGAHFLARSLDDALNLTEQPELANKVDMIWIVGGSSVYKEAMNRPGHLKLFVTRIMQDFESDTFFPEIDLEK*KLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_otogar Otolemur garnettii (bushbaby) AAQR03025778 synteny unknown
MVHRLNCIIAVSQNRGIGKNGDLPWPPLRNEFNYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESSQGAHFLAKSLDDALKHIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYRFEVYEKND

>DHFRL2_micMur Microcebus murinus (lemur) ABDC01288917 synteny unknown 
MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL3_micMur Microcebus murinus (lemur) ABDC01470170 synteny unknown two stop codons and a frameshift
MVHTLNCLVAVSQNMGIGKNGDLPWPPLRNEFKYFQKMTTTSSVEGKQNLVIMGKKTWFSIPEKN*PLKDRINVVLSREL*EPPQGAHFIAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAKNQPGHLKLFVTRIMQDFESDTFFPEIDMKKYKLLSECPGVLSDVQEEKGMKYKFEVYERnd

>DHFRL4_tupBel Tupaia belangeri (treeshrew) AAPY01242529 several candidates but synteny unknown retrogene shown two frameshifts
MVRPLDCIVAVSQmGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNTVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPRGAHFLAKSLDDALKLIEQPELANQVDMVWIAGGGSVYKEAMNQPGHLKPFVTRIMQDFESDTFfPEIDLEKYKLLPGYPGVLSDDQEEKGIKYKFEVYEEN

>DHFR_musMus Mus musculus (mouse) AK031241
MVRPLNCIVAVSQDMGIGKNGDLPWPPLRNEWKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD

>DHFR_ratNor Rattus norvegicus (rat) NM_130400
MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEIQEEKGIKYKFEVYEKKD

>DHFR_criGri Cricetulus griseus (hamster) NM_001244016
MVRPLNCIVAVSQNMGIGKNGDFPWPMLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELADKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFETDTFFPEIDLEKYKLLPEYPRVLPEVQEEKGIKYKFEVYEKKG

>DHFR_perMan Peromyscus maniculatus (deermouse) GH506523
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA

>DHFR_perPol Peromyscus polionotus (deermouse) GH462222
MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWVVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA

>DHFR_dipOrd Dipodomys ordii (kangaroo_rat) wgsBlast
MGRSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELNNKVDMVWIVGGSSVYKEAMNQPGHHKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKID

>DHFR_speTri Spermophilus tridecemlineatus (ground_squirrel) ti|1005446275
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMMTTPSVEGKQNLVIIGRKTWFSIPEKNRPLNDRINIVLSRELKELPKGAHFLAKSLTDALKLTEQPELASKIDMIWIVGGSSVYKEAMIQPGHIKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILSEVQEEKGIKYKFEVYEKTD

>DHFR_cavPor Cavia porcellus (guinea_pig) XM_003479518 Hystricognathi
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFGYFQRMTTTPSVEGKQNLVIMGRKTWFSLPEKSRPLKDRINVVLSRELKEPPQGAHFLAPSLDDALKLIEQPGLANEVDMVWIVGGSSVYKEAMERPGHIKLFVTRIMQEFESDTFFPEIDLKKYRLLPEYPGVPSEIQEEKGIKYKFEVYEKND

>DHFR_oryCun Oryctolagus cuniculus (rabbit) FJ687582
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLADALKLIEEPELAEKVDMAWIVGGSSVHKEAMNQPGHLRLFVTRIIQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_ochPri Ochotona princeps (pika) wgsBlast/ti|1497151195
MVRPLNCIVAVSQNMGIGRNGDLPWPPLRNEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPPGAHFLAKSLDDALKLIEEPELAERVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILPEVQEERGIKYRFEVYEKND

>DHFR_felCat Felis catus (cat) wgsBlast
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALELTEQPELAHKVDMVWVVGGSSVYKEAMNRPGHVRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDIQEEKSIKYKFEVYEKNN

>DHFR_canFam Canis familiarus (dog) XM_003432480
MVRTLNCIVAVSQNMGIGRNGDLPWPPLRNEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_vulVul Vulpes vulpes (fox) SRA|SRR094997.876315.2
MVRTLNCIVAVSQNMGIGRNGTVPWPPLRNEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_musPut Mustela putorius (ferret) AEYP01048652/AEYP01048654/AEYP01048651/SRA|SRR071690.159842.2
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEknd

>DHFR_ailMel Ailuropoda melanoleuca (panda) XM_002916218
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDIVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLDKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKND 

>DHFR_ursAme Ursus americanus (bear) GW292063 fragment
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKT

>DHFR_equCab Equus caballus (horse) XM_001504643
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND

>DHFR_vicPac Vicugna paca (vicuna) wgsBlast
MVRPLDCIIAVSQNMDMGKNGDLPWPplrNEFKYFQRMTTASSAEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLANSLDDALKVIEQPELTNKVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLSEYPGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_susScr Sus scrofa (pig) NM_001244064
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEYKYfQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLTEQPELKNKVDMVWIVGGSSVYKEAMNKPGHIRLFVTRIMKEFESDTFFPEIDLEKYKLLSECSGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_turTru Tursiops truncatus (dolphin) DT661408
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEYRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDGALKLIEQPELTNTVDMVWIVGGGSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLIPEYPGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_oviAri Ovis aries (sheep) GO728717
MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN

>DHFR_capHir Capra hircus (goat) JO612108
MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_bosTau Bos taurus (cow) NM_001077883
MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFQYFQRMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDVVWIVGGSSVYKEAMNKPGHVRLFVTRIMQEFESDAFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN

>DHFR_myoLuc Myotis lucifugus (microbat) wgsBlast
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNQVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE
 
>DHFR_pteVam Pteropus vampyrus (macrobat) wgsBlast
MVLSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSveGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_eriEur Erinaceus europaeus (hedgehog) wgsBlast/ti|909141827
MVRPLNCMVAVSQNMGIGKNGELPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDAALKLIDQPELTNKVDRV----------EVMNRPGHIRLFVTRILQEFECDTFFPEIDLKNYRLLP--------------------------

>DHFR_sorAra Sorex araneus (shrew) wgsBlast
MVRPLNCIVAVSQNMGIGKNGELPWPPLRNEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD

>DHFR_loxAfr Loxodonta africana (elephant) XM_003408117 error single trace TTTTTSSV
MVRPLNCIVAVSQNMGIGKNGDLP*PplRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRGLKEPPQGAHFLAKSLDDALKLIEQPELRNKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKRLSEYPGVLSDVQEEKGIKYKFEVYEKKD

>DHFR_proCap Procavia capensis (hyrax) wgsBlast
MVRPLSCIVAVSQNMGIGKNGDLPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD

>DHFR_dasNov Dasypus novemcinctus (armadillo) AAGV020342833
mVRPLSCYDAVYQNMGIGKNGDMPWPPLRNEYKYFQKMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELREPPQGAHFLAKSLDDALKLIEQPELADKVDMIWIVGGSSVYK---------------------------------------YPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_choHof Choloepus hoffmanni (sloth) genome/blat many pseudogenes
-----------------------------NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELK------------------------------------------EAMNRPGHLRLFVTRIMQEFESDTFFPEIDTEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_monDom Monodelphis domestica (opossum) XM_001363581
MVRQLNCIAAVSKNMGIGKDGDLPWPLLRNEFKYFQKMTTTPSVEGKQNLVIMGKKTWFSIPEKCRPLKDRINVVLSRELKEPPQGAHYLASSLDNALNLLELPELADKVDMVWIVGGSSVYKEAMNKPGHQRLFITRILQEFESDTFFPEIDLKKYKQLQDYPGVSPDVQEEKGIQYKFEVYEKHE

>DHFR_macEug Macropus eugenii (wallaby) wgsBlast
MAPTINCIVAVAQNMGMGKNGDLPWPPLRKEFQYFQKMTTTPSVEGKQNLLIMGKKTWFSIPEKNRPLKDRINLVLSRELKDPPLGAHYLAKSLDDALDLLRLPELANTVDMVWVVGGSSVYKEAMNKPGHQRLFVTRILQEFESDTFFPEIDLKRYKLLPEYPGVLTDVQEEKGIQHKCEIHEKHD

>DHFR_sarHar Sarcophilus harrisii (tasmanian_devil) AFEY01286966/AFEY01033989/AFEY01359230
MVRPINCIAAVSQNMGIGKNGDLPWPPLRNEFNYFQKMTTTSSVEGKQNLLIMGKKTWFSIPEKHRPLKDRINLVLSRELKEPPPGAHYLAKSLDDALDLLELPELADKVDMIWVIGGSSVYKEAMEKPGHQRLFVTRILQEFECDTFFPEIDLKKYKLLPEYPGVLTDIQEEKGIQYKFEVYEKHD

>DHFR_triVul Trichosurus vulpecula (brushtail) EG594753
MVRTINCIVAVSQNMGIGKNGDLPWPPLRNDFKHFQKMTTIPSVEGKQNLLITGKKTWFSIPEKSRPLKDRINVVLSRELKEPPPGAHYLAKSLDDALDLLELPELANKVDMIWVVGGSSVYKEAMNKRGHNRLFVTRILQEFESDTFFPEIDLKRYKLLXEYPGVLTDV

>DHFR_ornAna Ornithorhynchus anatinus (platypus) XM_001512186
MGPLLNCIVAVAKNGGIGNKGDLPWPALRNEFRYFQKMTTTPTVKGKQNLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLHLPELASKVDMVWIIGGSSVYKEAMEKGGHQRLFVTRILQDFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI

>DHFR_tacAcu Tachyglossus aculeatus (echidna) SRA:EUGXWLM02GHH9L/EUPZL4S02GL94B
MGRPLNCIAAVAKNGGIGNKGDLPWPPLRNEYRYFQKMTTTPTVEGKENLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLNLPELASKVDMVWIIGGSSVYKEAMEKPGHQRLFVTRILQEFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI

>DHFR_galGal Gallus gallus (chicken) NM_001006584 
MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEENGIQYKFEVYQKSVLAQ

>DHFR_melGal Meleagris gallopavo (turkey) genome/blat
------------QNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVE------------------------------------EAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLKEYPGVPADVQEENGIQYKFEVYQKSVLAQ

>DHFR_lagLag Lagopus lagopus (ptarmigan) GW704257
MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYQKSVLAQ

>DHFR_anaPla Anas platyrhynchos (duck) scaffold675
MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYEKTVLAQ

>DHFR_taegut Taeniopygia guttata (finch) XM_002190440
MPRSLNSIVAISQNMGIGKDGRLPWPPLRNDYKYFQRMTSASPVEGKKNAVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELQSKVCMVWIVGGSSVYKEAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKTVVP

>DHFR_ficHyp Ficedula hypoleuca (flycatcher) SRA|SRR029160.210264.2
MVRSLNSIVAVSQNMGIGKDGRLPWPPLRNEYKYFQRMTSTPRVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAVV*

>DHFR_melUnd Melopsittacus undulatus (budgerigar) SRA|SRR029329.119695.2
MVRSLNSIVAVCQNMGIGKDGSLPWPPFRNEYKYFQRMTSTSQVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELQETPKGAHYLSKNLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILQEFESDTF

>DHFR_allMis Alligator mississippiensis (alligator) genome/blat 
MVSSLNAIAAVSQNMGIGKNGTLPWPPLRNEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKT

>DHFR_croPor Crocodylus porosus (crocodile) genome/blat composite seq with alligator
MVSSLNAIAAVSQNMGIGKNGTLPWPPLRNEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV

>DHFR_chrPic Chrysemys picta bellii (turtle) AHGY01047456 AHGY01047458
MVRPLNCIAAVCQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTTVEGKQNVLIMGKRTWFSIPEKNRPLKDRINIVLSRELKETPKGAHYLAKSLDDALVLLDSPELKSKIDMVWIIGGSSIYKAAMEKPVHQRLFVTRILHEFESDTFFPEIDLEKYKHLPEYPGVPADVQEENGIRYKYEVYEKTV*

>DHFR_anoCar Anolis carolinensis (lizard) genome/blat XM_003216272
MVLSLNSIAAVCQNMGIGKNGQLPWPPLR 2NEFKYFQKMTMTPTQEGKQNVVIMGKRTWFSIPEKNRPLKDRINIILSREMKEVPKGAHYLARSLDEALDLLQSPELENKIDLVWIVGGSSVYK AAMDKPIHHRLFVTRILHDFESDTFFPEIDLEKYKLIPSYAGVPTDIQEENGIRYRFEVYEKNI

>DHFR_pytMol Python molurus (python) AEQU011279324/AEQU010134262/AEQU010204446/AEQU010142865/AEQU010435622/AEQU010656196
MVASLHSIVAVCNNMGIGKDGKLPWPPLRNEFKHFQKMTMTTKEEGKQNVVIMGKKTWFSIPEKHRPLKNRINVVLSKELKDVPEGAHYLAKSLEEALDLLETSEMERKVDKVWIVGGSSVYK AAMEKPIHHQLFVTRIMHDFESDTFFPEIDLKKYKLLPKYTGIPTDIQEENGIQYKFEVYENTI

>DHFR_ambMex Ambystoma mexicanum (axolotl) CN050864
MGRILNAIVAVCPSMGIGKDGNLPWPILRNEFKYFQRMTMTATQEGKQNVVIMGRKTWLSIPEKNRPLKGRINIVLSRELTAPPPGAHYLANSLDGALELLETRELAGKVDLLWIIGGSSLYRELIGRPHHQRLFVTRILKEFECDTFLPEFDLEKYRLLPEYPGVSLGIQEENGIQYKFEVYEKVEEA

>DHFR_xenTro Xenopus tropicalis (frog) CT025223
MRNPFLHAVVAVCPPNQGIGKEGSLPWPLLRNEFKHFQRLTMTPTVEDKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLAEAIDLLEDPELKDKVDLVWVIGGSSLYQELMEKPVNQRWFVTRILQEFECDTYLPEIDLNSFRLLPEYPGISPELQEENGVQYKFEVYEKIV

>DHFR_xenLae Xenopus laevis (frog) NM_001095037
MRNQFLHAVVAVCPPNQGIGKGGSLPWPLLRNEFKHFQRLTMTPTVEGKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLTEAIDLLEDPELKDKVDLVWIIGGSSLYQELMEKPINQRWFVTRILQEFECDTYLPEINLNSFQLLPQYPGISPELQEENGVQYKFEVYEKIV

>DHFR_ranCat Rana catesbeiana (bullfrog) GO463031
---------------------GGTLPWPLLRNEFKHFQRLTMTPTTEGKQNVVIMGRRTWFSIPEKNRPLKGRINIVLSRELTEPPNGAHYLAKSLDEAVHLLKTPEMKEKIDLIWIIGGSSLYKEVMDKPVSQRLFITRIQQEFECDTFLPDVDLQSYKLLPEYPGISSEMQEENGIQYKFEVYEKML

>DHFR_latCha Latimeria chalumnae (coelocanth) AFYH01150805/AFYH01150806/AFYH01150807/AFYH01150815/AFYH01150814
MGAARLLNSIVAVCPNLGIGKDGNLPWHPKRLSNEFRYFQKMTTTPTVEGKQNVVIMGRKTWFSIPEKNRPLKGRVNVVLSRELQEAPEGAHYLARDLDSALKLLESPELADRVDLVWIIGGSALYKEALEHPSQHRLFVTRVLQQFECDTYLPEINFNKFTLLPEFPGVSSAIQEESGIQYKFEVYEKNS
 
>DHFR_gadMor Gadus morhua (cod) FF410604 novel insertion 3 transcripts
MSRVLNCIVAVCPDAGIGYKGDLPWHPTRLNNEFKHFRRLTVTrtPGAEDKQNVVIMGRKTWNSIPEKNRPLNDRINIVLSRQLEAPPSGAHHLARDLPSALRLLDVPEGLLADQVWLIGGSSMYSEVMETPCPRRLFVTRILKQFPSDTFFPDISPAQFRLLPEYPGVSSELQEENGIQYRFQVYQSVDP

>DHFR_tetNig Tetraodon nigroviridis (pufferfish) CR710339
MARVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTWFSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVETQLAEQADQVWVIGGSSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQYRYQVYESLEP

>DHFR_hipHip Hippoglossus hippoglossus (halibut) EB031730 
MSRILNGIVAVCPDLGIGNRGNLPWHPVRLSNEFKHFRSMTATPSEKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKAPPAGAHHLSADFSSALRLVDTELADQADQVWVIGGSSLYKEMMESPGTRRLFVTRILKQFDCDTFLPEISPDKYRQLPEFPGVPQELREENGIQYRFEVYESIEP

>DHFR_solSen Solea senegalensis (sole) FF286406 
MSRVLNGIVAVCPDMGIGMTGNLPWHPVRLSNEFKHFRTMTATSSVKGKKNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKLPPAGAHHLAADFSSALRLVETELADEADQIWIIGGSSLYKEMMESPGIKRLFVTRILKQFECDTFLPEISLDKYRLLPEFAGVSQELQEENAIQYRFEVYESVEQ

>DHFR_oreNil Oreochromis niloticus (tilapia) GR620119
MPRVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTWYSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDTELADQADQVWVIGGSSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQYRFKVYESIQE

>DHFR_dicLab Dicentrarchus labrax (seabass) FM022259 
mSRILNGIVAVCPDLGIGMNGNLPWHPVRLGNEFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSKECKVPPAGAHHLAADFNSALRLVETELADQADQVWVIGGSSLYKELMESPGTRRLFVTRILKQFECDTFLPEICPDKYQLLPEFPGVSQELQEENGIQYRFEVYESTEQ

>DHFR_perFla Perca flavescens (perch) GO655828 
MSRILNGIVAVCPDLGIGNNGNLPWHPVRLNNEFKHFRTMTATPSVNGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRECKAPPAGAHHLARDFSSALRLVDTELAQQADTVWVIGGSSLYKEMMESPGTRRLFVTRILKQFECDTFLPEITPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIQQ

>DHFR_spaAur Sparus aurata (seabream) FM155123 
MSRIVNGIVAVCPDLGIGNNGNLPWHPVRLNREFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPDKFRPLNNRINIVLSRECKVPPAGAHHLAADFSSALSLVDTELADQADQVWVIGGSSLYKELMESTRTSRLFVTRILKQFECDTFLPEISPDKYSLLPEFPGVPQGLQEENGIQYKFEVYERIKQ

>DHFR_gasAcu Gasterosteus aculeatus (stickleback) ENSGACT00000004939 
MSRVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDTELAEQADQVWVIGGSSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQYRFEVYESIDV

>DHFR_oryLat Oryzias latipes (medaka) BJ496706
MTRTLNGIVAVCPDLGIGKGGNLPWHPLRLSKDFALFRKMTSTPLVAGRQNVVIMGRKTWFSIPEKNRPLQNRINIVLSRQLKAPPAGAHFVAADFAAALRLVDSELAERADQVWIIGGSSIYXEMMESPAPRRLIVTRVQKQFECDTFFPEIRPEQFRLLPEFPGVSQELQEENGVSYKFEVYESIQQ

>DHFR_anoFim Anoplopoma fimbria (sablefish) BT082890
MSRVLNAIVAVCPDLGIGRNGDLPWHPVRLNNEFKHFRRMTSTPSVEGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKTPPAGAHHLAQDFNSALRLVDMKLAEQVDQVWVIGGSSLYKELMESQGIRRLFVTQILKQFECDTFLPKISPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIEQ

>DHFR_esoLuc Esox lucius (pike) BT079933
MSRVLNCIVAVCPNMGIGNKGNLPWHPKRLNNEFKYFQKMTMTPFVEGKQNVDIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPPGAHHLASDFSSALRLLDTAEMAERVDQVWVIGGSSLYKEMMESEGIRRLFVTRILQQFESDTFLPEINQDKYRLLPEFPGVPQDVQEESGIQYRFEVYESVDH

>DHFR_salSal Salmo salar (salmon) BT047529
MSRVPNCIVAVCPDVGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNVVIMGRKTWFSIPERNRPLKNRINIVLSRELKKPPAGAHHLASDFRSALCLLDMAEMAERADQVWVIGGSSLYKEMMESQGTRRLFVTRILQQFKSDTFLPEINQDQYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ

>DHFR_oncMyk Oncorhynchus mykiss (trout) EZ786723
MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ

>DHFR_danRer Danio rerio (zebrafish) NM_131775
MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH

>DHFR_cteIde Ctenopharyngodon idella (carp) CK233011 
MSRILNCIVAVCPDMGIGRKGNLPWHPIRLSNEFKHFQKMTMTPSVEGKKNVVIMGRKTWFSIPAQNRPLKNRINIVLSRELKTAPEGAHYLASDLSSALHLLDSSELEKQVDQVWIIGGSALYKEVMERSGPRRLFVTRVLKQFDCDTFIPEINMDKYKLLPEFPGVPTGLQDENGVQYVFECMIVS

>DHFR_cypCar Cyprinus carpio (carp) CA968873 
MSRILNSIVAVCPDMGIGKNGNLPWHPIRLSNEFKHFQKMTMTPLVEGKKNVVLMGRKTWFSIPAANRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSSELEKQVDQVWIIGGSALYKEVMESSGPRRLFVTRVLKQFDCDTFIPDINMDKYKLLPEFPGVPTGLQEENGVQYVFEVYESTDH

>DHFR_ictPun Ictalurus punctatus (catfish) FD330522 
MGRVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYESVEQ

>DHFR_leuEri Leucoraja erinacea (skate) EE989413 
MTRLINSIVAVCPNMGIGNNGNFPWHPIRLSKEFKHFQRMTSTPSVEGKCNAVIMGRKTWHSIPEKNRPLKNRINIVLSRELKEVPEGAHYLAPDLDSALALLDSSDLQDKVDLVWIIGGSALYKEALESPASHRLFVTRILQDFDSDTFIPEINLNKFKLLPEFPDVPADIQEENGIRFKYEVYERISPV

>DHFR_squAca Squalus acanthias (dogfish) DV497998 
mPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA

>DHFR_eptBur Eptatretus burgeri (hagfish) BJ646201
MAQNPVNVIAAVLPNMGIGWKGGLPWHSKSLVKEMKHFTRLTSAAAEGKQNAVVMGRKTWESIPEKFRPLRNRINVVLSRTLSHLPLGAHYLATDFSNALAQLSESELCCRIDHIWIIGGSSIYKEALLHPACHRIFITRIMHDFEADTFFPHIDSKRFSLCQSYPGIPEEIQEENGVQYKFEVYEVVAK

>DHFR_petMar Petromyzon marinus (lamprey) Contig6033:1-27,714
GKQNVVIMGRKTWFSIPEKKRPLKNRINIVLSRELKCLPEGAHYLVPDLNSALSLIDSTPAIQEKLDGVWILGGGGVYKEAMEHSACRRLFITRVLQTMEADAFFPDIDADKFKLLP

Urochordate, cephalochordate, hemichordate and echinoderm reference sequences

>DHFR_cioInt Ciona intestinalis (tunicate) F971791 dozens of transcripts
0 MPAKDIQIHSVVACCNNGGIGFKGRLPWRLP 2
1 KEMKYFKRITTGEVEEFG GRRNaiIIGRKTWESIPKSFKPLKDRINIVISRTLDKDCEGP 0
0 DLVVNSLDELIdLLHSKPWCDKINQVFNIGGNEIYK 0
0 LIHNSQYCGKIYLTRVLADlQCDTFLENLDDNFTKLPTEG 2
1 FPEVPQGIQMDKNDLQWKVEVYEKKTT* 0

>DHFR_cioSav Ciona savignyi reftig_155
0 MPAKELKIHSIVACCNNRGIGNKGRLPWRLP 2
1 KEMKHFTSLTTGDVENLN GKRNAVVVGRKTWESIPKSFKPLKNRVNIVISRSLSKEYEGP 0
0 DAVVNSLEELVQLLQSDHWSNRINQVFNIGGSEVYK 0
0 LINNSKYCGTIYLTRVLADFECDTFLDKFDDTFKQLDTTK 2
1 FPEVPQGEQMDKNDISWRVEVYVKKS* 0

>DHFR_halRor Halocynthia roretzi (squirt) DB618016/EY457317
0 MKIHAVVACCNNRGIGKDGTLPWRLP 2
1 KEMAHFQKITKGSPPK  GKKNAVVMGRKTWEEIK   RPLKDRYNIVMSRSLSEKLKGM 0
0 DALVRSLGELEDLLSSGEWTGEINEVFNIGGAEICR 0
0 LINGSSLCGRLCVTQMVTDFECDAFLPEIDDGEYLR LDE 2
1 YPEDVATLLPDGEVVEANGVKWAVEVYEAI* 0

>DHFR_botSch Botryllus schlosseri (tunicate) HO068783
0 MEIHTVVVCDDNWGISKDGCLPFGTK 2
1 KEAAHFYRFIADTSHLPP GRKNVLIMGRKTWTE ETPADAPQSRSVTYNFLLSKTLKERPEEV 0
0 DGIVSSVEEFVTLMESPRWKDKIGRIYNIGGGQACE 0
0 AIYASKYCGRAYMSLVRGDFHCDTFAPNLD

>DHFR_molTec Molgula tectiformis CJ399083 poor quality fragment
MGRNTWNSIPSKFRPlKnRWNIVLSRTlKDPyDGM
HAVVSSlDQlYKlLQDEAWKDKVNEIFCIGGAQVYE
VIDNSPYIGLiFMTRIRKKYDCDTFYSEVNKDKFILLKSCP

>DHFR_oikDio Oikopleura dioica (tunicate) CABV01002330/FP801526 completely different intronation from Ciona
0 MNKSGWNMILAADIKGGIGLRNDLPWRIPQDLKHFQMLTKGTPNQESVVVMGRNTWQSIPEKFRPLK 0
0 GRVNVVITNTLESENFIKCTSLEAAIAYINENHVNATKWIIGGAtLYNRGLKENFIDEVYLTRIQGDY 1
2 NCDTYVKDLYKILEVDYRLETSPKFDSKEVIGESGIKYAFERYVKNSGI* 0

>DHFR_braFlo Branchiostoma floridae (amphioxus) ABEP02022512/XM_002597864/BW702700 triplicated contig misassembly
0 MKTKKLSLVVAACNNMGIGVDGKIPWTLR 2
1 GDMKFFSRLTSGTEEA 1
2 GKQNAVVMGRKTWFSIPDRFRPLPKRLNVVLSRNLT 2
1 TPPEGAHHLAGSLEEAVKMLTETTVADTIDKVFIIGGNSVYK 0
0 DALSHPCCHRVYLTRVYKDFHCDTFFPNMDDTFKLVS 2
1 DPTIPSEMQEENGIEYKYEVYEKVA* 0

>DHFR_sacKow Saccoglossus kowalevskii (acornworm) ACQM01022605 exons 2-3 fused
0 MKKISLVAAACNNMGIGKNGDLPWRLR 2
1 KEMSFFTKVTSETKED GKQNAVVMGRKTWFSIPEKYRPLAGRYNVILSKNLK 2
1 ECPSGADMLSESLDDAIKQLSEPPLVDKIDKVYIIGGSSVYK 0
0 ESLESAACYRIYLTRVMADFDCDTFFPEFDTNLFNLVS 2
1 DPDVPSEIQEEKGIQFKYEVYEKK* 0

>DHFR_balCla Balanoglossus clavigerus (acornworm) FN986971
mQKISPVAAACNSMGIGKNGNLPWRLRKEMKYFTNVTSETVEEEKQNAVIMGRKTWFSIPEKYRPLNNRFNVILSNTLKECPAGADLLSPSLEEAVKLLSESPLEGKIDKVFIIGGSSVYKESIQSSLCHRIYLTRIMADFDCDTFFPNFDTNIFKLISDPSIPSEIQEEKGIQFKYEVYERQDV

>DHFR_strPur Strongylocentrotus purpuratus (urchin) AAGJ04143548  exons 2-3 fused
0 MAEKKLNLIAAACTSkGKMGIGINGNLPWRLR 2
1 QEMAYFERLTKTAQME GMKNAVIMGRKTWDSIPEKFRPLKDRVNVVLSNSLT 2
1 ECPPGADHLCSSLNEAVKLFSSPPLSETVDMVWITGGSAVYK 0
0 DGIDSPHCHRIYLTRIMKEIECDTFFPEFDLDRFKLVT 2
1 DPAVDQDTQEEKGIQYKFEIYESSS* 0

>DHFR_parLiv Paracentrotus lividus (urchin) AM567148
MADKRLNLIAAACTSNGKMGIGINGNLPWRLRQEMAYFERLTKTSQMEGMKNAVVMGRKTWFSIPEKFRPLKDRVNVVLSSSLTECPPGADHLCSSLSEAVKLLSSPPLSQTVDMVWITGGSSVYKDGIDSPLCHRIYLTRIMKEIECDTFFPEFDLDRFKLVTDDAVSQDIQEEKGIQYKFEIYESS

>DHFR_lytVar Lytechinus variegatus (urchin) JI431194
MAEKKLNLIAAACTSSGKMGIGINGNLPWRLRQEMAYFERLTKTPQLEGMKNAVIMGRKTWFSIPEKFRPLKDRINVVLSNTLTECPSGADHLCSSVSEAVKLLSCPPHSQTVDMLWIIGGSAVYKAGIESPCCHRIYLTRVLKEIECDTFFPEFDLEKFKLVVDPAVSQDIQEEKGIQYKFEIYENSS

>DHFR_patPec Patiria pectinifera (starfish) DB389444/DB385866 also called Asterina
MAGQKQcNLIVAACKCKDSLGIGINGTIPWKLRTDMKFFSTQTSTTAEN DKKNAVIMGRKTWLSIPDKFRPLPNRVNIVLSKTLSECPAGADHLCRSLDHAVKLLSEPPVADGIDVVWIIGGSSVYKEAMESSLCHRIYLTRIHSQFECDTFMPEIDTSKFKVVSDPAVSGERQKENDIEFNFEIHQNIQHTSTS

Protostome reference sequences

>DHFR_droMel Drosophila melanogaster (fruitfly) NM_001043255 Diptera; Muscomorpha 2-6 fused 
MLRFNLIVAVCENFGIGIRGDLPWRIKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILEEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKILEKHS

>DHFR_droSim Drosophila simulans (fruitfly) XM_002103136 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVCENFGIGIKGDLPWRIK 2
1 SELKYFSRTTKRTSDPTKHNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILEEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKVLEKQS
	
>DHFR_droSec Drosophila sechellia (fruitfly) XM_002041171 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVCENFGIGIKGDLPWRIK 2
1 SELKYFSRTTKRTSDPTKHNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILQEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKVLEKQS
	
>DHFR_droEre Drosophila erecta (fruitfly) XM_001980159 Diptera; Muscomorpha 2-6 fused
0 MIRFNLIVAVGENFGIGYKGDLPWRIK 2
1 SELKYFSRITKRTNDPSKHNAVVMGRKTYFGVPESKRPLSNRLNVVLSTTLQENELPEGVLLCSNLETALKVLENKNDVENVWIVGGSGVYKEAMASPRCHRLYITKILHKFECDTFFPAIPDSFREVAPDSDIPLGVQEENGIHFEYKVLEKQSK

>DHFR_droYak Drosophila yakuba (fruitfly) XM_002097560 Diptera; Muscomorpha 2-6 fused
0 MLSFNLIVAVCENFGIGINGNLPWRIR 2
1 SELKYFSRTTKRTSDPSKHNAVVMGRKTYFGVPEGKRPLPDRLNIVLSTTLQENELPKGVLLCRNLEAAMKALEDKNEVENIWIVGGSGVYEEAMASTRCHRLYITKIHEKFECDTFFPAIPDSFREVAPDSDTPLGLQEENGIKFEYKVLEKQ

>DHFR_droAna Drosophila ananassae (fruitfly) XM_001954865 Diptera; Muscomorpha 2-6 fused
0 MRRFSLIVAVSENLGIGIKGDLPWRIK 2
1 SELKYFSRTTKRTSDPSKRNAVIMGRKTYFGVPESKRPLPNRLNIVLSTTLQPEDLPKDVMLCPNLEAAMKHLEGDDQIENVWVVGGSGVYAEAMDSNRCHRLYITRIHQKFECDTFFPTIPESFREVAPDPDTPAGVQEENGIEYEYKVMEKETK

>DHFR_droPse Drosophila pseudoobscura (fruitfly) XM_001359349 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVSENFGIGLKGDLPWRLK 2
1 SELKYFSRTTKRTSDPNKRNVVIMGRKTYFGVPASKRPLPERLNIVLSTTLKPAELPSDVVLCHSLEEAMKHLEEETLRDQIENVWIVGGSGVYEEAMASSRCNRLYITKIQQNFECDTFFPSIPDSFREVPPDADTPVGVQEENGIKYEYKVLEKRE
	
>DHFR_droPer Drosophila persimilis (fruitfly) XM_002017170 Diptera; Muscomorpha 2-6 fused
MLRFNLIVAVSENFGIGLKGDLPWRLK 2
1 SELKYFSRTTKRTSDPNKRNVVIMGRKTYFGVPASKRPLPERLNIVLSTTLQPAELPSDVVLCPSLEEAMKHLEEETLRDQIENVWIVGGSGVYEEAMASSRCNRLYITKIQQNFECDTFFPSIPDCFREVPPDADTPVGVQEENGIKYEYKVLEKRE

>DHFR_droWil Drosophila willistoni (fruitfly) XM_002070058 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVCENSGIGLKGDLPWRIR 2
1 SELKYFSNTTKRRLDSSKRNVVIMGRKTYFGVPPSKRPLPDRVNVILSSTLKSNELPNEVLIYSSLEGAMQELEKQKDQIENVWIVGGSSVYAESMASPRCHRLYLTKIYAEFECDTFFPKIPESFREVSPDKDTPPGIQEENGLKYEYKVFEKQE

>DHFR_droMoj Drosophila mojavensis (fruitfly) XM_001998634 Diptera; Muscomorpha 2-6 fused
0 MLKYNLIVAVCENFGIGFKGDLPWRLK 2
1 SELKYFSRRTKRINDPSKRNVVIMGRKTYFGVPPSKRPLPDRLNIVLSTTLTQKELPEGVLLCGSLDEAMQTLEKKPLCDQVENIWIVGGSGVYAEAMESPRCHRLYITKIQERFECDTFFPHIPASFHEVELTEDTPHGVQEENGIKFEYKILEKREHII

>DHFR_droVir Drosophila virilis (fruitfly) XM_002056347 Diptera; Muscomorpha 2-6 fused
0 MLRYNLIVAVCENFGIGLKGDLPWRLK 2
1 SELKYFSRTTKRTRDPAKRNVVIMGRKTYFGVPPSKRPLPDRLNIVLSTTMSRAELPNDVLLCRSLEEAMKMLESESEPWCDQIENIWIVGGSGVYEEAMASPRCYRLYITKIQEQFECDTFFPNIPADFHEVPLDEDTPHGVQEENGIKYEYKILEKQ

>DHFR_droGri Drosophila grimshawi (fruitfly) XM_001990307 Diptera; Muscomorpha 2-6 fused
0 MLKFNLIVAVCENFGIGFKGNLPWNLK 2
1 SELKYFSRTTKRVCDPSKRNVVIMGRNTYMGVPPSKRPLPDRLNIVLSTTMSAADLPKEVLLCRSLNEAMETIESPEWCKQVENVWIVGGSRVYAEAMDSERFHRLYLTKIHEKFECDTFFPEIPDCFKEIPLDEHTPDGIQEEDGIKYEYKILEKQ

>DHFR_gloMor Glossina morsitans (tsetse_fly) EZ423753 Diptera; Muscomorpha
MLKFNLIVAVSKNFGIGLKGGLPWELKSELRYFSELTKRVFDSTKRNVVIMGRKTYFGIPLNNRPLRNRLNIVLSTTLNKVGELPEEVLLQPNLEAAMKFLEDNNTLKSNIENIWIIGGASVFKEAMASKRCHRLYITEIQSSFESDVFLPTIPNDFEQIIPGPEVPQTVQVENCICFRYKVLEKRE

>DHFR_haeIrr Haematobia irritans (buffalo fly) AY099474 Diptera; Muscomorpha
MLKFSLIVAVCENFGIGIKGDLPWLLKSELKYFSTTTKRVHDPSKRNVVIMGRKTYFGVPESKRPLQQRLNIVLSTTLKQSDLPKDVLLYPNLESAMSFLENEKDINQQIETVWIVGGSGVYKEAMASPRCHRLYLTKIYAKFDCDTFFPDIPADFKEVDLDPETPKGIQEENNITYEYKILEKQDHK

>DHFR_sarCra Sarcophaga crassipalpis (flesh_fly) EZ609788 Diptera; Muscomorpha
MLKFSLIVAVCENFGIGIKGDLPWKIKSELKYFSSTTKRVRDPSKRNVAIMGRKTYFGIPESKRPLPERLNIVLSTTLKPSDLPSDVLLYPNLESAMKMLEENEKLKQEIETVWIVGGSGVYAEAMASQRCHRLYLTKIQASFECDTFFPKIPDDFVEVELDEETPKGIQEENNIKYEYKILEKKT

>DHFR_culQui Culex quinquefasciatus (mosquito) XM_001864570 Diptera; Nematocera exons 2-6 fused
0 MKKFSLIVAVCSNGGIGIKGDLPWRLR 2
1 SELRHFARMTKRVADPGKRNAIIMGRKTYFGIPEGRRPLPDRLNVVLTRDPASNTYPEGVLVCTSLEDALAKIQDLVENIWIIGGSAVYKEAMESRHCHRIYLTEILAPFECDAFFPEIGKEFRLVGNDADLAEEVQEENGVRFQYKIYEKKDID

>DHFR_anoGam Anopheles gambiae (mosquito) XM_319471 Diptera; Nematocera exons 2-6 fused
0 MSKKFSCIVAVCENRGIGINGDLPWKLK 2
1 QELKYFSHTTKKVNDADKRNAVIMGRKTYFGVPESKRPLPERLNIVLTRNAAAYTFPPDVLVCGSLQEALHKLDSTDHGEHIENIWVVGGNSVYKEAMESDRCHRIYLTEIKKQFDCDAFFPEIPNSFMVIDNDPDTPSGVQEENGIQYVYKIYENKQQ

>DHFR_aedAlb Aedes albopictus (mosquito) X60192 Diptera; Nematocera exons 2-6 fused
0 MKKFSLIVAVCANGGIGIKGDLPWRLR 2
1 QELKYFSRMTKKIQDSGKRNAIIMGRKTYFGVPESKRPLPERLNIILTRDPSANAYPSEVMVCTSMQEALKKLDEAPLVNEIENVWIVGGNAVYKEAMQSDRCHRIYLTEIKETFECDAFFPEITSDFQLVKNDDDVPEDIQEENGIQYQYRIYEKVPK

>DHFR_aedAeg Aedes aegypti (mosquito) AY432005 Diptera; Nematocera exons 2-6 fused
MKKFSLIVAVCANGGIGIKGDLPWRLR 2
1 QELKYFSRMTKKITDTSKRNAIIMGRKTYFGVPESKGPLPERLNIILTRDPSANTYPSDVMVCKSMQEALTKLDESPLANEIEKIWIVGGNAVYKEAMQSERCHRIYLTEIKETYECDAFFPEITSEFQLVKNDDDVPEEIQEENGIQYQYRIYEKLPK

>DHFR_armSub Armigeres subalbatus (mosquito) EU205282 Diptera; Nematocera
MKKFSLIVAVCANGGIGIKGDLPWRLKQELKYFSRMTKKKQDTSKRNAIIMGRKTYFGVPESKRPLPERLNIILTRDPSAHVYPPDVMVCTSMQDALNRLDETPLVDEIENIWIVGGNAVYKEAMQSARCHRIYLTEIKKTFECDAFFPEINSDFQLVKND

>DHFR_danPle Danaus plexippus (monarch) AGBW01009470/AGBW01006850 Lepidoptera
0 MSKVKLNLIAAACENMGIGSNGSLPWRLK 2
1 KEMEYFTTMTTKVKDA SKINAVIMGRRTWDCIPNKNRPLENRLNIVLTRHVDVIKDK 0
1 KEMAYFTTMTTSVSDK KKVNAVIMGRRTWDCIPIKYRPLSNRINIVLTHNVDYVKKQ 0
0 VPKDVVVVSGLDEAVSYIIDRNDVESTWVIGGSSVYQ 0
0 ASMDHPNCGKIYLTEIQKTFDCDTFFPNINRQRFHLVDEDDIPRDIQTEGDISYYFRVYKRL* 0

>DHFR_bomMor Bombyx mori (silkworm) BABH01041437+BABH01041438 Endopterygota; Lepidoptera
0 MSRTQLNLIAAACENMGIGINGTLPWKLK 2
1 KEMAYFTTMTTSVSDK KKVNAVIMGRRTWDCIPIKYRPLSNRINIVLTHNVDYVKKQ 0
0 VPEGVIVVPGLDEAIDHIDSRSDIESTWVIGGSSIYK 0
0 AAMEHPNCGKIYLTEIQKSFDCDTFFPDINKQRFHLVNEEEVPGEKQSEGDINYYYRIYKRL* 0

>DHFR_helVir Heliothis virescens (budworm) AF104106 Lepidoptera
MSQVKLNLIAAACDNMGIGVNGALPWRLKKEMAYFTTMTSKVSEPTKVNAVIMGRRTWDCIPDKYRPLQDRVNIVLTHNVDSVKENVPEGVMVFPGLDEAIKYIEGREDIESTWVIGGSSIYRAAMTHPNCGKIYLTEIQKSFDCDTFFPNIDKQQFHLVDEEQIPGEKQVEGNISYYFRVYKKL

>DHFR_triCas Tribolium castaneum (beetle) AAJJ01000197  Coleoptera exons 2-4 fused
0 MVIKFDLIAAACENMGIGKNNDLPWRLK 2
1 SELAFFSQMTTQTSDESKKNVVLMGRKTWDSIPPKFKPLHQRFNFIMSRAGVNLEGYKDCFSFKSLDEVISKLQDEKFQQLYENVWVIGGSYIYE 0
0 ATMASKYFHRLYLTKVLKTFDCDTFFPKIRDDLIEVRYL 2
1 DPRVPEGVQEENGIQFVYHVYQNPDFNKL* 0

>DHFR_denPon Dendroctonus ponderosae (beetle) GO487443 Coleoptera
 MALKLNLIVAASENWGIGKNGTLPWKLKKEMAFFRTMTSATEDKSKKNVVIMGRRTWESIPAQFKPLPNRINFVLSRSGLNLDQYSNTQSFTNFREALDKLEESAFRQLYENVWIIGGSSLYNETFRSPHFHKLYLTKIHKEFDCDTFLP ALPGNLKEINDPAVTED   IQEENDIEYTFQVFEVIN

>DHFR_apiMel Apis mellifera (bee) HP575683 Hymenoptera
0 MNLHLIAATCEGMGIGIKGTLPWKLK 2
1 SELAFFTYMTTNTKNPNKRNVVLMGRRTWESIPKENRPLKNRINIVLTSQSL 2
1 DYGNDVIVCKNIPHAFEVIEKIKDRIENIWVTGGSSVYK 0
0 EAMESPNFYRLYLTRIKKYFECDTFFPTIPNDFVLTE 2
1 DPNIPQGIQEENDIQFVYEVYKKNRFLIWFQNNIFRIL* 0

>DHFR_bomImp Bombus impatiens (bumblebee) JI114531 Hymenoptera
0 MNLNLIAAACEGMGIGVKGTLPWRLK 2
1 SEMAFFTSMTTNTKDPNKKNVVLMGRRTWECIPKKYKPLKNRINIVLTSQSL 2
1 DYGDDAIVCKSIPHALEVIKKPTLKDQIEGIWVIGGSSVYK 0
0 AAMESPNFHRLYLTRIKKHFVCDTFFPTIPNNFVLID 2
1 DPNIPQGVQEENDIQFVYEVYEKNRFLYLKII

>DHFR_eugCor Euglossa cordata (bee) HP973249 Hymenoptera distal frameshift fixed
MSLNLNIIAAVCEGMGIGVKGSLPWKLKSEMAFFTSMTTQTKDPK KNVVIMGRRTWECIPTKYRPLKDRINVVLTSQNLDYGVNAIICKSMSHALELLKKPPFSDEIESVWVIGGNLVYKAAMESPNFHRLYLTRIKSILTVIHFFPPIPNDFALIQDPNIP

>DHFR_nasVit Nasonia vitripennis (wasp) XM_001605731 Hymenoptera
0 MQVKLKLIAAACENMGIGVNGDLPWRLRK 2
1 EMDFFTKMTSTTKDENKKNVVLMGRRTWESIPKKFKPLSNRINMVLTSQPL 2
1 DLGEDAIVCKNLPEAIEKISQSPLKDKVEQVWVIGGSLVYK 0
0 TSMESPNFYRLYLTRVKKDFKCDAFFPEIPNDYILVK 2
1 DPEVPEGIQEEKDIKFEYEVYEKKQN

>DHFR_copFlo Copidosoma floridanum (wasp) JI834972 Hymenoptera
MQVKLKLIAAACDNMGIGINGDLPWRLRKEMDFFTKMTSTTKDPNKQNVVLMGRKTWESIPKKYKPLANRINVVLSSQPLDLGKDAIACNSLPEAIQQISQDHLRDKVEQVWVIGGSSIYQAAMDSPNFYRLYLTRVKKHFDCDTFFPEIPKDYALVKDPEVPEGIQEEKGINFEYEVYQKA

>DHFR_attCep Atta cephalotes (leafcutter-ant) ADTU01012310  Hymenoptera
0 MPPKLELIAAACENMGIGVNGNLPWRLK 2
1 AEMAYFTRMTTNTRNKNKRNVVLMGRRTWECIPEKYRPLKDRINMVLTSQSL 2
1 NYGDDAIACKSILHALDVISEMQNEVERVWVIGGSSVYK 0
0 SAMESPHFGRLYLTRIKKKFECDTFFPSIPNDLVLIE 2
1 DSTVPQGIQEEKGIEFVYEVYERR* 0

>DHFR_camFlo Camponotus floridanus (carpenter_ant) XP_393902 Hymenoptera 
MPPKLELIAAACENMGIGINGGLPWRLK
TEMAFFTRMTTDTKDKDKKNIVLMGRRTWDCIPEKYKPLENRINMVLTSQSL
NYGDKAIICKSIPHAIEVIAEMQNQVERVWVIGGSRVYK
DVMGSPYFGRLYLTRIKKEFKCDTFFPPIPSGFVLIK
DPTVPQHIQEEKGIKFIYEVYEKK 

>DHFR_harSal Harpegnathos saltator (jumping_ant) XP_393902 Hymenoptera 
MLPKLELIAAACENMGIGIKGDLPWRLK
TEMEYFTRMTTETKDKNKKNVVLMGRRTWDCLPETYRPLRNRINMVLTSQL
MNYGDKAIVCTSIPNALEIITKIQNEVERVWVIGGSKVYK
EAMESPYFGRLYLTRIRKRFECDTFFPPIPNNFVLVQ
DPIVPEDIQEEKGIQFVYEVYEKR

>DHFR_linHum Linepithema humile (argentine_ant) LH21356-RA Hymenoptera
MQLKLELIAAACENMGIGNNGALPWRLK
TEMEYFTRMTIDTKDKNKKNVVLMGRRTWDSIPIKYRPLNDRINMVLTSQSL
DYGDKAIVCKSIPHALEVIAGMQNQIERVWIIGGSSVYK
DAMESPHFGRLYLTRIKKEFECDTFFPPIPNDFVLVT
DPAVPQDVQEEKGIKFVYEVYEKR

>DHFR_pogBar Pogonomyrmex barbatus (harvester_ant) B10193-RA Hymenoptera
MSHKLELIAAACENMGIGINGDLPWRLK
TEMAFFTRMTTETNHTNKKNVVLMGRRTWECIPDKYRPLKDRINMVLTSKSL
DYGNETITCKSISHAFDIITQMQNQIERVWVIGGSSVYK
SAMESPYFGRLYLTRIKKKFECDTFFPPIPNDFVLIE
DPTIPQGIQKENGIEFVYEVYERR 

>DHFR_solInv Solenopsis invicta (fire_ant) AEAQ01011575+AEAQ01011576 Hymenoptera
MPPKLELIAAACENMGIGVNGDLPWRLK
TEMAFFTRMTTDTKQNNKKNVVLMGRRTWECIPKKYRPLKDRINMVLSSQSL
DYKDEAIVCKSIPHALDVISGMQNQVERVWVIGGSSVYK
SAIESPYFGRLYLTRIKKKYECDTFFPLIPNDFVLVE
DPTVPQGIQEENGIEFVYEVYERR

>DHFR_bemTab Bemisia tabaci (whitefly) EZ942456 Hemiptera
MSSLRLSIIVAMTADNGIGFKNSLPWSLPNELRNFAKLTKNCKDKSKQNAVVMGRNTWESIPAQHRPLKDRLNVVLSSTLKDAGEGVLVCPRLKDALTLLEENYQNKIENVWIIGGARVYEDALNLSNCHKLHVTHIQEKPECDVFFPTIPRDFQIITTSEMVEENGFHYNYQVYERRTSPDGEANGIEDSTKS

>DHFR_acyPis Acyrthosiphon pisum (aphid) NM_001162022 Hemiptera one exon
MVYSVIAAVSKNGGIGYKGNLPWKIKKEMEYFNLMTTRVNLKGVQNAVIMGRCTWQSIPDKYRPLKGRINVVISKTLNSVPEGVLLYPKLEEALKSLYLNDRIENLWVIGGSGLYKEAVNDKNCKKLFITKIDQEYLCDTFFPDFDTKKFEETSEANVPKGIQEDNGIKYEFKVFKRL

>DHFR_blaGer Blattella germanica (cockroach) FG125641 Orthopteroidea
MNLKLXLIVAVSENMGIGLNGDLPWRLRKELAHFSRLTKRTIDSNKQNAVIMGRRTWESIPIKNRPLPERLNVVLTKQELDFGKEVLVCNSLNSALEKLCEPRLDKTIESIWVIGGSSVYKEAMSSPHCHRIYLTKIFKTFECDTFLPEIPGNSFKIVRDPDVADEIQEENGLTYEYQVYERILV

>DHFR_pedHum Pediculus humanus (louse) XM_002432121 Phthiraptera
0 MVNNFNLIAAVCSNNGIGYKGNLPWNLR 2
1 KELQYFNRMTKDVKNPEKKNAVIMGRKTWDSLPHNWKPLPGRYNFVLTTQSL 2
1 NLDGAVVCSDLNDLIKKINSPDYSNLIETAWVIGGSKVYESVLKYGLCHRFYLTRIKKEFECDCFFNYDFTKDFKEVS 2
1 DDRVPKGVQKENEIEYVFHVYEKVNESKDSSN* 0

>DHFR_onyArc Onychiurus arcticus (springtail) EW753645 Hexapoda; Collembola
MGPKLYIIAAACENMGIGYKNDLPWKLRNEMAYFNRMTQIVENPGSGSELKNAVIMGRRTWDSIPEKFRPLRNRLNIVLTSRPDFKQPNVLSYPGWYEAVKDLATPEKSLQIDKVWVIGGNSTYKLAVESPDCHRIYLTRVLKHFECDAFFPEFDKSKFRLANDPNVPQDIQEEDGIQYHFEI

>DHFRcalCle Caligus clemensi (sea_louse) BT080999 Crustacea; Maxillopoda; Copepoda
MTSLKCVKINVIAAACKSSRGIGKDNDLPWKLPTDMKYFREKTASTTEIRVGVIMGRRTWESVPPKFRPFKNRFNVVLTSREDFEAKGAVIARTLEDAVKKCSNNVDSIWVIGGSGVYAEALNKYPCRVYLTEIDKEFDCDAFFPPLDEKKYKLASVSPDHQENDITFRFKVYDVIEL

>DHFR_lepSal Lepeophtheirus salmonis (sea_louse) BT121746 Crustacea Copepoda
0 MSKSIPTLHVIAAACRPTNGIGKNNTLPWNLPTDLKYFKTTTSSTPDSKVAVIMGRRTWESIPSKPRPLKNRINVVLTTNSNFVAEGAILA
SSLEEGIKSCMDQADKIWIIGGANLYKEAMQYYKCQIFLTEIDKEFECDTFFPSIDSTKYKEVSVSEDHVENGITFRFKRFERLLQENN

>DHFR_litVan Litopenaeus vannamei (shrimp) FE141783 Crustacea Malacostraca 
MSVRLNIIVAACENHGIGINGELPWKLREEMKYFSRMTKATKSLEKQNAVMMGRKTWESIPAKFRPLPGRLNLVISSRAKDPGPEFNGSTVCASFKEAIDEVQSRTDEVESLWAIGGSSIYE
MALESEHLHRIYLTRILKDFECDTFLPLFDPKKFQIVTD
PAVPAELQQEGDITYKYEIYEKVK

>DHFR_celPug Celuca pugilator (crab) JO488787 Arthropoda; Crustacea
MGPRLNIIVAVAENHGIGKGGELPWRLREEMKHFSRMTKRLDSPATQNVVLMGRKTWESIPLKFRPLPGRLNVVLSSQMKQQQDSSGKVIVCQDFESALKSVSTENPEVETVWVIGGHSVYKMALESEHLHRIYLTKVMKEFECDVFFPSFKMNEFKLVEDPDVPGQV

>DHFR_dapPul Daphnia pulex (water_flea) FE400591 Crustacea Branchiopoda
0 MKLNLIVATASNMGIGFQGTIPWRLK 2
1 KDMALFAKLTKNTNDTNKQNAVVMGRKTWESIPEKNRPLSNRLNIVLSSSQQ 2
1 IENTLTCKSLESAMQLLQTPPYLDQIENIWIIGGASVYK 0
0 EAMEHPSCHRIYVTHILKDFECDVFMPAIDPAKFSLVG 2
1 DSKVPEGTEEENGISFNVKVYEKV* 0

>DHFR_ixoSca Ixodes scapularis (tick) EW930272 Chelicerata Arachnida 3 re-intronated exons
0 MCPGVQESLQYFAIAAMCHNRGIGVLNTLPWRLKKEMAYFSRITSQAAEGKTNAVVMGRNTWDSIPPKYKPLPGR 2
1 VNVVVSRTLEKVPDGHHVARSFSDALSLLQPLVDSGK 0
0 VDKTFVVGGAQLYREALADPRCTRVYLTEIDADFECDVFFPELELGQFRLVQEEGVPQEEQTEGGLTYRFNVYQRISSGSV* 0

>DHFR_ambMac Amblyomma maculatum (tick) JO841362 Chelicerata Arachnida
MCPSHKSVVSCFAIAAMCRNRGIGFKNALPWRLKKEMAFFKRMTSEAAEGKQNAVVMGRNTWESIPPKFRPLNNRINVVVSKTLTEVPEGHHVASSFPAALQLLQTLVDTGKVDKVFLVGGAQLYREALESGHCTRIYLTEVDKDFECDVFFPEFDNSVFSPVEEEGVPQEPQQEDGVTFRFRVYERVQN

>DHFR_varDes Varroa destructor (mite) ADDG01075186 Chelicerata; Arachnida one exon
MAVPPLCAIVAVCKASKGIGNKGSLPWGRSLPNEMKHFARVTTNTTDPDKCNAVVMGRRTWESIPENRRPLPRRINIVISSGLCQESVPEGVKVAKSFDEALEIAGTGCCKGQSRVERIFVIGGTQVYVDAMRHPAMDTVYLTEVLGQFECDTFLNIDTRKFTDIEDPAVTNEEQEENGIRYKYRVLKKIPQ

>DHFR_perSed Peripatopsis sedgwicki (velvetworm) FN242195 Onychophora 
MHRYLNLVVAVCNNLGIGIGGELPWRLRGDMKFFSKLTSETKDSEKTNVVIMGRKTWASIPEKFRPLPKRTNIVISSSLNSVPEGVLIARDFEHAMSLAFGLQQDNIVEQVFVIGGSSVYEQALKSDFPCRIYLTRVMGDFKCDTFFPKLNVDKYALVKVPEIPEEVQEENGISYLFQVLQEKVES

>DHFR_milTar Milnesium tardigradum (tardigrade) EZ761524
--------VAACRNGGIGIRGDLPWTLRGDMRFFTKITSQTKDPQKKNAVIMGRKTWFSIPERFRPLSKRINVVISRTMFGIQPNAIQTQSLEEALDLLSKEPLSSEIETVWIIGGESLYKMAIHSPQLYRVYFTNVMADFECDTFFPTQELQRMKVVSDADVNGDVQMENDVKFQHEIYEP 

>DHFR_triSpi Trichinella spiralis (nematode) ES273161 Nematoda Enoplea
MAQVNIIVAICEKYGIGKKNSLPWHLSKEMQHFKKMTTSVSDPNKINAVIMGRNTWYSIPEKYRPLSGRFNIIISGTMPQLSDEDVIVTWHWREAIEIVEHPISKKAIETFWICGGAKLYNDVIESGLWNRLYVTWIMKEFDCDAFFSFPDKNTIKLVEDDRIPSDIQVEKGIHYKYEVYENCMKMQS

>DHFR_xipInd Xiphinema index (nematode) CV509322 Nematoda Enoplea
MNLIVAACDNMGIGHGNELPWPKLPNESKHFLKLTAGTRDPGKQNAVIMGRKTWEIIPVEHRPLKHRLNVVISRTLSRKDVPPGVQLFEDFEPAMRYLLSPQVSQNIETIWNVGGSEIYALGLDHPSFHRLYFTRVSGTYDCDTYFPQVDWTMLKRVEDPNISTGLHEENGVQYKFETYEHAF

>DHFR_caeEle Caenorhabditis elegans (nematode) NM_059963 Nematoda Chromadorea 4 exons re-intronated
0 MRKMNLIVAMDAEGGIGKNGVLPWRIKKDMQYFASVTKNVSDQSK 0
0 RNAVLMGRKCWESIPVTRRPLAGRLNIVLSRQLPAQKSDDYIVV 2
1 NSLEAAMKLLSEPPFVDSIETIWNIGGAEIYDLALRENLVDEIHLTRIFKN 2
1 FEADVHLKSLDFSKMEKVQNAEVSSENSEIFEENGLKFEFCKWKVVENH* 0

>DHFR_melInc Meloidogyne incognita (nematode) CF803120 Nematoda Chromadorea 3 exons re-intronated
MNIIAAVDENFGIGKNNSLPWRLPKEYKHFINLTTTTKNPNKINAVLMGRKCWESIPEKYRPLKNRLNIVMTKTWVTPEF 0
0 VGENLIFINSLDSLNLILESKPYENLIETIW 2
1 NIGGKQIYSLGIEHQNLNKIVLTKIDKNFDCDVKFPEINWNEFIEEENGEIVEEKGLCWKAITYIKK* 0

>DHFR_ascSuu Ascaris suum (roundworm) JI215038/AEUI01062849/AEUI01051380 Nematoda Chromadorea
0 MVSPKLPINIIVAMDSRGGIGKNGALPWHIPEDLKYFQTMTTKTIDPTKQNAIVMGRKVWESLPAKWRPLKNRLNVVLSNS 0
0 MDDPHDGSYIVTRTFESAIDILNGMSDKIETIWDIGGRRPYEEGLKSSQLRQ 0
0 LYVTFVEGDFDADVFFPDVDFKKFSKQNGDKQSSEHHYEGITYRFETFTALHTDTELS* 0

>DHFR_schMed Schmidtea mediterranea (planaria) DN307116 Platyhelminthes Turbellaria 6 standard exons
0 MKRLNLIVAACENQGIGKNGKLPWNLK 2
1 NEMIYFNNITTSVSDS 1
2 NKQNVVIMGRITWESIPNKFRPMPKRINIVISSTLSR 2
1 DHDNLTGTYLFNNLSEALSFTNDELSDKVEEVFVIGGSSLYK 0
0 AALSSSYPLRLYYTKIFESLDCDTFFPAFEWEQFKSVK 2
1 LDHVPSEVQQENGIRYQFMVLDRC* 0

>DHFR_schMan Schistosoma mansoni (tapeworm) XM_002580511 Platyhelminthes Digenea
0 MRLNVVVAVSENWGIGKGGGLPWKIK 2
1 KDMEFFKTVTTKAHP 1
2 GLKNAVVMGRVTWESIPESFKPLKDRINIVVSSTLSH 2
1 APSFVQVVPSLNAAIDLLYNEEFSSIVDEVFIIGGYRLYK 0
0 EALKQS YPVRIYCTHILSEVDCDTYFPKVDWDKLKK 2
1 VDLPDIPADTFTENGFTFKFCVYDVPSEQFI

>DHFR_taeSol Taenia solium (tapeworm) PEL761437 Platyhelminthes Cestoda
MGLRRLNVIAAVAKNGGIGKENKLPWHIREDMAFFSRITSTAQEGKKNAVVLGRRTWLSFPPKFRPLPNRVNVLVSTQLESVPEGAYLVKSFEDSLRLVESLVDGGEVDEVFIIGGHGLYKEALEQEVYPVRLYYTHIMKDFDCDTFFPSVDWERFTPIQLDTVDSNPKYCGEIEFRFAVYEKGPHRFHDN

>DHFR_aplCal Aplysia californica (sea_hare) AASC02008512 Mollusca Gastropoda one exon
MTSTKLNIVVAVCTNMGIGIEGRLPWRLKQDMAFFKQLTVETQDEQKKNMVIMGKKTWMSIPTKFRPLQDRVNVVLSTQLTEPPSGALLASSLKEAVNLAKSDANVENVFVIGGASVYREAVEGDCPCRIYLTRVDKEFECDTFFPKFDTDVFERIQNPNNVPTGINVEGDLSFTFEVYEKNLFPH

>DHFR_lotGig Lottia gigantea (limpet) FC707856 Mollusca Gastropoda one exon 
MCTKRLNIVVAACKNNGIGVNGSIPWRLKKDMAMFRHITSDTVDESKQNAVIMGRKTWMSIPDKFRPLKNRVNIILSNTLSESPDGTYIVKNFQQALSLVNSDKMKQQIESVHIIGGSSVYKEAMESDYDCRVYLTKVDADFECDTFLPPIEESLFQRVKNPSNIPSDVQEENGIQFTYEIYDKTTDQSDRESPVSR

>DHFR_phyAcu Physella acuta (snail) BW985974 Mollusca Gastropoda 
MPKLNIIVAACNNNGIGIEGRLPWRLKSDMSFFKQITLRTQDAEKKNAVIMGKNTWFSIPSKFRPLVGRINVVLSTQMTDAPEGTQLAKSLNQAVSLLESLNVENIFVIGGSTVYKQAMEGEHPCRIFLTRIAADFLRDTFLPDIPN

>DHFR_pinMax Pinctada maxima (oyster) GT283724 Mollusca Bivalvia
MSKCKLNLVVAACNNRGIGIDGQLPWRLRKDMDFFKKITTETNDPEKRNAVIMGRKTWFSIPEKFRPLSKRINIILSREMKEVPENTYVARSLDEAVQLVSDGGELADRVESIHIIGGSSVYKEALASSHPCRIYFTRVLADFKCDTFLPEFDENKYQKLENMENIPTEIMTEKDINF

>DHFR_mytCal Mytilus californianus (mussel) ES395435 Mollusca Bivalvia
MSKTKSTLNLVVAACNSRGIGINGKLPWRIRKDMDFFKKITMETKNPDKKNVVIMGRKTWFSIPEKFRPLPKRINIILSREMKEAPPGVYIARSFDDAITMVNKDLSDKAESIYVIGGSSIYKAAMDSDYDIRIFLTKILADYDCDTFLPQFDENLYKIIENYEGVPKGKQTENDIDFIFEVYEKVR

>DHFR_dreRos Dreissena rostriformis (quagga_mussel) EY433849 Mollusca Bivalvia
MSMPRLNLVVAICNNNGIGINGSLPWKLRKDMDFFKTITMTTTDPEKQNAVIMGRKTWQSIPENFRPLRNRINIILSRDMNAPNGTHVARSLPDALSLLSSEELADRVEQIHIIGGSSVYKEAMTGPHPCRLYLTRILADFECDAFLPEIDDSSFTKIRNPDNVPTDLQEENGLKFRFEVYDKL

>DHFR_alvPom Alvinella pompejana (bristleworm) GO125274 Annelida Polychaeta 
MPPTLNLIVAMCNNNGIGIQGKLPWKIK
GDMAFFRKMTTETKNPAKMNAVIMGRKTWFSIPEKNRPLNKRMNIILSQTMSSVPDGVHLARSFSEALNLISTKPLADIIEGIFVIGGASVYKEALESSYPCRIYLTRVFADVPCDTFMPTFSGFKQIECAEIDNTVQKENDWQYKFEVYEKV

>DHFR_helRob Helobdella robusta (leech) EY386022 Annelida Clitellata six exons
0 MQPKLQIVVALCVKNRGIGLNNSIPWKLP 2
1 GDMTFFRKLTSETSIL 1
2 GSKNAILMGRKTWDSIPSNLKPLKNRLNVVISRTLE 2
1 cPDInGRLHVSKSFEDAVEFVASDKSIDKIFIIGGSSIYD 0
0 LALASSTCYNVFITEISNDFQCDTFFPKFNQDAYKLIK 2
1 YPGHSTSVQFENGIGYQFTCHHKVSK* 0

Cnidarians, ctenophores, trichoplax, sponge and choanoflagellate reference sequences

>DHFR_nemVec Nematostella vectensis (anemone) ABAV01013911 Cnidaria six exons
MAAVGVRFSCIVAMDLKRGIGKNNDLPWKIK
GDMKFFTEKTSEVKTE
GKQNAVIMGRKVWESIPEKFRPLKGRLNIVLSRTLS
EPPQGAQLCRSFEQALTILSTDPYTKKIENVFVCGGSALYK
DAMAHSACTRIYITYIDQEFDCDVFFPEFDQNTYHLVE
DPDVPSVQHEEKGIKYKFCVYDRCQ

>DHFR_acrMil Acropora millepora (coral) EZ020360 GO002175 Cnidaria Anthozoa Hexacorallia 
VAVADNGGIGKENRLPW HLK
GDMKFFSHLTSTVATE
GKQNAVIMGRNTWESIPKKYRPLPRRLNIVLSRKMS
EPPEGAVLCSSLQDALDRLRSAPYTDTVEKVFVIGGATVYE
EAMQHHQCYRLYITHIYKDFECDVKFPEFDKRIYKETR
DIDVSSEMLEEKGIPYEFKVYQKD

>DHFR_hydMag Hydra magnipapillata (hydra) XM_002157178 Cnidaria Hydrozoa one exon
MVQLRKVHCIAAIDSEWGIGLKGKLPWNLPREYKFFQNITTKVISEGKQNAVIMGKNTWFSIPQQHRPLKNRLNVILSSSFIKEDYPKNVLLESSLEAAILRLSDEFYANTVENIFVIGGSRVYKEAMEKFCDKIYLTKIEQDYSCDVFYPIFDTNKFKEIDDEEVDKNKQIENGVSYTFHVYSSNYTV

>DHFR_mneLei Mnemiopsis leidyi (sea_walnut) AGCP01012173 Ctenophora exons 2-3 fused
0 MKLRFNIIVATDLEGGIGKNNNLPWRCR 2
1 KEFRHFTDTTSKLQNGKQGAAVMGRKTWESIPEKFRPLKNRHNFVISSTLS 2
1 VANPGNVPVYKTISDCMTALDQDKD IGSVWIVGGWGIYK 0
0 EAMESERLHRMYITTVQGKFDCEVFFPSFDERNFNLVI 2
1 DPDIPNETVTEDNGISWSVSVYERK

>DHFR_triAdh Trichoplax adhaerens (placozoa) ABGP01000098 same six exons
0 MQKNFRMIAAMTRDRGIGYKNDLPWKLR 2
1 NEYKYFARLTTSTKDS 1
2 AKKNAVLMGRNTWVSIPEKNRPLRNRINIVISSQLR 2
1 AEEVPEGVHVVASIPEVIELVQSPLLKDIVETVWIVGGAPVYK 0
0 GFMSHPQCDRIYLTKLDANYECDVFFPEMSDDFKEIS 2
1 DPDVSQEVQEENGLKYTFHVYQRN* 0

>DHFR_subDom Suberites domuncula (sponge) GH558547 Porifera Demospongiae
MYIMVAKEIKSGIGNKGKIPWPRINTDMNFLETMTKKTRNKTKQNAAIMGRLTWESIPAKYRPLPERFNIVLSSKKFEDIAPGAHMVCSSLSFAMEKLRAPPLSDQIENVWIFGGAGVYEESMKHPDCDKLYVTEVLQEFPCDAFCPPIDSDIYKLIDHSDDPDVPKGEFVENGVKFKFEVYQRQ

>DHFR_monBre Monosiga brevicollis (choanoflagellate) ABFJ01001321
0 MRVALVVAATKAGGIGHQGQLPWHLK 2
1 ADMQHFRDITTKTEQP 1
2 AAINAVIMGRKTFESIPDKFRPLHGRLNVVLSTQKP 2
1 VSLPLHTPHDTNPPQPLPHPACLAHPQPEVIETAYIIGGASVYN 0
0 EALAKPEYSHYIFMTKVHKDVLCDTVMDMKHLDAYEHDPNFPVq 2
1 * 0