Blat Scripts

Here is a collection of Blat-related Perl scripts that perform functions that are frequently requested on the genome mailing list. If anyone finds a problem with these scripts then please notify me.

BlatBot.pl: This is a script that takes a file of FASTA format sequences as input and then submits them to the web-based Blat on the UCSC Genome Browser web site. It obeys the site rules for the number of frequency of hits i.e. Program-driven use of the Genome Browser software is limited to a maximum of one hit every 15 seconds and Blats sequences in batches of 25 sequences at a time. Link title

The script usage is: usage: BlatBot.pl <organism> <db> <searchType> <sortOrder> <input FASTA> <outputType> <output file>

       Specify organism using the common name with first lettercapitalized.
       e.g. Human, Mouse, Rat etc.
       Db is database or assembly name e.g hg17, mm5, rn3 etc.
       searchType can be BLATGuess, DNA, RNA, transDNA or transRNA
       sortOrder can be query,score; query,start; chrom,score;
       chrom,start; score.
       outputType can be pslNoHeader, psl or hyperlink.
       blats will be run in groups of 25 sequences, all
       output going to the specified output file.