Opsin evolution: Neuropsin phyloSNPs

Neuropsin backgrounder

Neuropsin (OPN5) is a deeply diverged member of the opsin family with a single publication on it and considerable confusion over the name (mostly used for an unrelated kalikrein serine protease KLK8, not an opsin). There are no known disease associations or described knockout phenotypes; it is expressed primarily in brain, spinal chord, and testes.

Neuropsin has all the classical attributes of a rhodopsin-class GPCR and indeed opsin photoreceptor: Schiff base lysine at expected position, standard tyrosine counterion and DRY motif, seven transmembrane configuration, disulfide at expected position, proximal glycosylation and distal palmitoylation and kinase sites. It is most closely related to peropsin and rgropsin in terms of blast clustering and intron positioning. Its G-protein signalling partner is not known.

The evolution is illuminated by the massive comparative genomics study described here, which extractes and compares over 50 full length sequences from various genomics projects. Neuropsin can be located outside chordates but not outside deuterostomes. However, like peropsin and rgropsin, it must have originated much earlier in pre-Bilaterans. Thus its absence in earlier diverging species must be due either to gene loss or unrecognizability.

Within placental mammals, neuropsin is extraordinarily conserved, with percent identity relative to human protein 96% averaged over 31 species (likely above the 95% percentile proteomewide). That conservation drops considerably at marsupials and monotremes (86%), is less striking at tetrapods (78%), and unremarkable at teleost fish (68%). This pattern suggests neuropsin acquired significant new adaptive functionality on the placental mammal stem, leading to marked resilience to fixation of any further variation.

The structure of the neuropsin gene is rather odd. In human, a weak splice signal appears to have developed that results in a seventh very short exon. However a stop codon is encountered if the splice is not made. This results in two slightly different alternative carboxy termini sequences QVE vs XXX. Very few transcripts exist in this region for any species but it appears that the recent ancestral form of the protein only utilizes the stop codon in exon 6.

Novel neuropsins in amphioxus and sea urchin

The genome of Branchiostoma (amphioxus, lancelet) contains two distinct neuropsins about 75% identical to each other and 42% to human. These cluster unambiguously with vertebrate opsins and share critical conserved residues. An extra intron distinguishes them from the vertebrate neuropsin pattern. Recall Branchiostoma species has three rather diverged (and well-studied) peropsins but no evident Rgr opsin. These raises the question whether neuropsin and peropsin developed substantial visual roles in this species as an alternative to the ciliary imaging opsin pathway seen by lamprey divergence. Sea urchins, but not acornworm Saccoglossus, contain a single neuropsin that is quite diverged.

These neuropsins are newly reported here, meaning they were not localized in recent in situ hybridization studies. That's especially unfortunate in view of the antecedent role the Branchiostoma ancestral node plays in the evolution of chordate eye and the complexities of photoreceptor tissues in the extant species.

PhyloSNPs in vertebrate neuropsins

Alignment analysis coming shortly. Neuropsin has rather few of them.

position  ...................................................................................................1.........1.........1.........1.........1.........1.........1.........1......1..
position  .........1.........2.........3.........4.........5.........6.........7.........8.........9.........0.........1.........2.........3.........4.........5.........6.........7......7..
position  123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567..
excMemCy  eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMcccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeee..
keyResid  ...GLC.................................................................................................diS..Y....................DRY...............................................
exonNumb  111111111111111111111111111111111111111111122222222222222222222222222222222222222223333333333333333333333333333333333333333333333333333333334444444444444444444444444444444444444..
10homSap  MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPE..
11panTro  ...................................................................................................................................................................................
12gorGor  ............................................................................................................................................------------------.....................
13ponPyg  ...................................................................................................................................................................................
14nomLeu  ...................................................................................................................................................................................
15rheMac  .................................I............................................................................................................................................A....
16papHam  .................................I............................................................................................................................................A....
17calJac  ......S.......................................................................................................................................................................A....
18tarSyr  ....................................................................................I.........................................................................................A....
19otoGar  ............LR..........................................................V...................................................................----------------------..M......E.......
20micMur  ......V.................................I..........................................---------------------------------------------------------..............L....V..............A....
21tupBel  ............S.............................................................................................................................................V...................A....
22musMus  ....................E..................................................................................F..................................................V...................A....
23ratNor  ....................E..................................................................................F..................................................V...................A....
24speTri  ............H.......E................................................................................................................................F....V...................A....
25dipOrd  ..F....GT.GQG.....PEE...T........I........................................................................................................................V........................
26cavPor  .......P..N.H..R..Q.E...V.......................V...........................I..................R.............................V.................................V..............A....
27oryCun  ............H......E..............................................................................................................................R.......L...................A....
28ochPri  ....D.......H....F.........................................................................................L....D.....................------......R.......V...................A....
29canFam  .......R...........E........................................................I.............................................................................V...................A....
30felCat  .......P...........E.................................................--------------.......................................................................V...................A....
31bosTau  .......P.P...R.P........................................................V...I................................................................I............V.....A.............A....
32turTru  ......................K..........I.............................................................................V...................A....
33susScr  .......P.P...R.....E....................................................V...I.............................................................................V.....A.............A....
34vicVic  .......P.P...R.RH...............................L..................................................M........................................-------------------------------------..
35equCab  ............................................................................I.............................................................................V........................
36myoLuc  ............G.....Q.............................V.......................V...........................................................................T.....F........................
37pteVam  ......V.....H....V..............................V...........................I.............................................................................V........................
38sorAra  ............N..........................................................................................M.................................................VV...................A....
39eriEur  .S..Q.......G.........................................................................................................................................L...V...................A....
40loxAfr  .T.....P...D...Q..Q.....T.................................C..............................V................................................................V...................A....
41proCap  .T....V..E.D..S.........T................V......C.........Y..............................I...S..........................................H...-------------------------------------..
42echTel  .......P...NS...........V.........G.....I.................Y....................................S.......T..................................................V........................
43dasNov  ...........D...............................................K..............................................................................................V........................
44choHof  ......G....DS....F......................I....................R............................................................................................V.............L..........
45monDom  .....SVS...DYI..............................V...L.....I....K............V......................S.......V................................H....T....H..F....L.....T..A.V.FA.V.S.A....
46macEug  .V...L.....I....K............V..............................V................................H....T....H......VI.....T..A....A...N.A....
47ornAna  MT.YS.PQLGDY......E....V............V..V...V...L.....I.................V..................V...........M................................H....T....H.......I........A........N.A....
48galGal  ..SDCNSSS.E.Y....MQQE........R...II......V......L.....IF...K............V................S...F.S...I...M................................H.A..T....H..F....L.....T..A.V.FA.V.S.A....
49taeGut  ..SEYNNSS.E.YI....QEE........R...II.............L.....IF...K............V................S...F.S...M......C.............................H....T....H..F....I.....M..A.V.FA.V.S.A....
50anoCar  .EQGQNISS..DN----QQEE........V...I...V..LV......L.....I...TQ.....K......V................S..AF.S...I...S.............I..............F...H....T....H.VF...GI..S..A..A.I.FA.F.N.A....
51xenTro  ..G.SSYREESGYI...E..S........R...IF..V..MA......L.....I..ACS........................T....A.V...S.......NA..................L.V..........H.R..T....R..F.A..V.....TL.A.L....V.N.A....
52danRer  .E-NET-SISSGYI....LR.........K...I..A..ILV.....AT.....M..TFK..T..K.P....L...IF.F....S....F.V.S.S...L...Q...Y.................I..F.......H.R..T....H..FLSVVF.....A..A...V..W.N.A....
53pimPro  .E-NDT-SIPSGYV....LR.........K...I..A..ILV..V..AT.....I.QTIK..T..K.P.F..L....F.F....T....F.V.S.S...L...Q...Y.................I..........H.R..T.F..H..FL..VFT.L..A..A...V..W.N.A....
54takRub  .E-NET-WTHSSYV....LR......R..K...I..AL.IC...LM.AT........TFK..T..K.P.L..L...IF.F....T....F.V.SLS...L...E...F.................V..........H.R..A....H..FL...SV....A..A......W.S.A....
54tetNig  .D-NET-RSHPSYV....LR......R..K...I..A..IF...VM.AT........TFK..T..K.P.L..V...IF.F....T....F.V.SLS...L...E...F.................V..........H.R..T....H..FV...LV....A..A......W.S.A....
56gasAcu  .E-NET-WTHPSYI....LR......R..K...II.A..IC....M.AT.....I..TIK..S..K.P.L..V....F.F....T....FVV.S.A...L...E...F.................V..........H.R..T....Q..FL..VFV.M..A..A......W.N.A....
57oryLat  .E-N.S-W.HSSYV....LR......R..K...I..A..IL....M.AT.....I..TIK..S..K.P.L..V....F.F....T....FVV.S.S...L...E...F.................V..........H.R..T....Q.IFL..VFV.I..A..A......W.S.A....
59calMil  .TFDNSTALYSGYWL.DSLH....V........IISAC..IVT.L...L.....I.L.ITQ.R..K.P..LIT...IS.F.M..G.Q..L.....S...I...V....H................V..........H.Q..S..Q.R.VFMS..F..F..A..A......W.N.A....
10homSap  MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPE..
phyloSNP  ..................AA......B......A..A..B.....B.BB.........B......C.A.........B.A....A..........B......................................A...B..A.......A.............A...............
  ..
  ..
position  1.1.........1.........2.........2.........2.........2.........2.........2.........2.........2.........2.........2.........3........3..........3.........3.........3.........3.....3
position  7.8.........9.........0.........1..................3.........4.........5.........6.........7.........8.........9.........0..................2.........3.........4.........5.....6
position  89012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456
excMemCy  eeeeeeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMccccccccccccccccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMcccccccccccccccccccccccccccccccccccccccccccccc*
keyResid  ...diS................................................................................................................K.PP........................................
exonNumb  44444444444444444444444444444444444444444444444444444444444444444444444444455555555555555555555555555555555555555555555555555555555555555555555555555555555556666666666666666666666
10homSap  PFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKS-LEGFRLHTVT-TVRKSSAVLEIHEEV*
11panTro  .................................................................................................................................................E.....-..........-...............*
12gorGor  ...............................................................P.....................................K.................................................-....Q.....-...............*
13ponPyg  .....................................................................................................S.................................................-..........-...............*
14nomLeu  .......................................................................................................................................................-..........-S..............*
15rheMac  .......................................................................................................................................................-..........-...............*
16papHam  .......................................................................................................................................................-..........-...............*
17calJac  .......................................................................................................................................................-..D.......-...............*
18tarSyr  ......................................................G.........T......................................................................................-..D.......-...............*
19otoGar  ..-...........-.L...............................................G................................................................................T.....-..D.......-............Q..*
20micMur  .......................................................I.............................................................................----------------------H......-A...........Q..*
21tupBel  ....................I..................................I..........................................................................................................-............Q..*
22musMus  ................G.......S.................A...........................V...............................................................R.....A...RG.....-..D.......-............Q..*
23ratNor  ................G.......S.................A...........................V................................N..............................R.........R......-..D.......-A...........Q..*
24speTri  .......................-..............E..............E..............-------.......................................S....................................-..D.......-A.......V...Q..*
25dipOrd  ................LA.................S...........................P.......................................................................................-.......................Q..*
26cavPor  ................A...I...H...........M..................I.............................................................................SR.....NA.........-..D.......-.D...-......Q..*
27oryCun  ........................................................................................................................................S..R.S.........-..D.......-............Q..*
28ochPri  ................................................................G.......................................................................S..R.....Q.....-..D.......-............Q..*
29canFam  ................L...I.........................................................................................................................R........-..D...N...-............Q..*
30felCat  ....................I..................................................................................................................................-..D.......-............Q..*
31bosTau  ....................I.................................................V................................................................................-..D.......-..........V.Q..*
32turTru  ....................I..........................................P..........................................................V............................-..D.......-............Q..*
33susScr  .....................................................................................V.................................................................-..D.......-...........RQ..*
34vicVic  ---------------------------------------------------------------------------............................................................................-..D....A..-............Q..*
35equCab  ....................I...........................................G.....V................................................................................-..D.......-............Q..*
36myoLuc  ...............T....I.................................K.........-----------.............................................S..............L........R......-..N.......-............Q..*
37pteVam  ....................I...........................................G..M.........................................................................S..R......-..D.....I.-...EA.......Q..*
38sorAra  ....................I..................................................................................N..............................R.....S...R......-.DD.......-...E........Q..*
39eriEur  ................L...I.................................K............M..............................................................................N....-.KDY...................q..*
40loxAfr  ....................I..................................I...........M............................................................................R......-..........-..K.......V.Q..*
41proCap  ---------------------------------------------------------------------------..............................V.................................R.R..R...E..-...V......-............Q..*
42echTel  .......................................................I...........M............................................................................................I.-...........HQ..*
43dasNov  ................................................................................................................................................R......-..D.......-...E......V.Q..*
44choHof  ...................................................................M.......................................................................R....R......-F.........-............Q..*
45monDom  .................A..A.V.S.......F.............L.....T.....Y.T..QN..I.................................Q.....V.F.......................C......S..Q..A..E.-.RTY......-...R........Q..*
46macEug  .................T..T...............................T..........Q..............................................................................................RHTVSTIRKSSSVSETYQ..*
47ornAna  .................A..A...............................T..........QN....................................Q.......F.......................CRIS..RL..P.TG..E.-.KNS.S.SMS-.I..P...SGP.Q..*
48galGal  .................A..A.V.S.......F.............L.....T.....Y.T..QN..I.................................Q...V...F.......................C.....RS..P.TLQ...S.KES.MY.IS-SH.D.A.LSGTQL..*
49taeGut  .................A....V.S.....................L.....T.....Y.T..QN..I.................L...................V...F......................ECRL...RP..*
50anoCar  ..............G..A..A..........V......M.C........Q..T.....Y.T..QNQ..................MF...................V..KV..I.............V......C.S...RP.N.QPLQ..NSR*
51xenTro  ....T............K..I.V.SM......F..M......A.........A.......T.NQNN.T..I.................F............Q......E......MM....S...........C.P...KKD--.SLQNTT----S.VY.IS-.F...TTSAR*
52danRer  ............T....S..S.VMCM.....IF..VI......M..F.....A...S...T.NKNN.S............................M....E...V..PV..........S............C.KK.VKSCCFQ.WR..KPSKTS.FY.ISGSIKQRPGD-.ASI.I*
53pimPro  .................S..S.VMCM.I...V...G.......M..LQ....AQ..S...TQNKNK.H............................V....D...V..SI..........S............C.KN.AKLSCFQ.WS.RKHYKTS.FYSISASMK.RP.N-.VPT.I*
54takRub  .................S..S.VMA......I...GI......M..F.....A..IS...A..RN..D..I.........................I....E...V..PV..I.......S..........V.V.TS.TNFSCC..L.ERIHFRKS..Y.ISGSL.DPLPPK.A.I.M*
54tetNig  ....A............S..S.VMA......V...GI.....IM..F.....A..IS...A..KN..S..I.........................V....E...V..PV..I.......S..........A.L.TS.TSSSCC..L.ERVLFRKA..Y.ISGSL.DTLPPK.A.I.M*
56gasAcu  .................S..S.VVA......V..AGI......M..F.....A..ISN..A..KN..N..I.........................V....E...V..SV..I.......S..........L.L.NS.MKSSCF.GL..PRHFRKS.FY.ISGS.KDNTTAK.AQI.M*
57oryLat  .................S..S.VMS......V..AGI......M..C.........SS..A..KN..T..I.........................V....E......PV..I.......S.........LV.L.NS.-S.CCA.VIR.RTHFRNS.FY.ISGSLKDTAPAK.A.I.I*
59calMil  .............RV..S.LI.V.T.........III.....I.........A..........QNH.S...N..........................................................................................................*
10homSap  PFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKS-LEGFRLHTVT-TVRKSSAVLEIHEEV*
phyloSNP  ...........................................B............B......CA.......................................................B.................................B...B.BBA............A..*

Curated Set of 51 deuterostome neuropsins

>NEUR_homSap OPN5 1_6 43 0 1  2_6 40 2 1  3_6 57 2 1  4_6 112 2 0  5_6 81 0 2  6_6 19 1 0
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR_panTro
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKETKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR_gorGor
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 12 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 YASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIPSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGKPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFQ 2
1 LHTVTTVRKSSAVLEIHEEv* 0

>NEUR_ponPyg
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGSPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR_nomLeu
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTSVRKSSAVLEIHEEv* 0

>NEUR_macMul
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADIVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR_papHam
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADIVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEv* 0

>NEUR_calJac
0 MALNHTSLPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR_tarSyr
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VIGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKGVAHFDSRIHTSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHEEv* 0

>NEUR_otoGar
0 MALNHTALPQDELRPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 WTMMPLVGLEDYVPEPFTSCTLDWWLAQSLGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKTTKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR_micMur
0 MALNHTVLPQDERLPHYLRDGDPFASKLSWEADLVAGFYLIII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2  1
2 GVWLKRKHAYICLALIWAYVSFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDHR 2
1 LHTVTAVRKSSAVLEIHQEv* 0

>NEUR_tupBel
0 MALNHTALPQDESLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHVLEMKLTK 0
0  2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR_musMus
0 MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQAGGLRGTKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR_ratNor
0 MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 gVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPNSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQTGGLRATKKKSLEDFR 2
1 LHTVTAVRKSSAVLEIHPEv* 0

>NEUR_speTri
0 MALNHTALPQDEHLPHYLRDEDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAFICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFINILFFCLLLPTAVIEFSYVKIIAKVKSSSEEVAHFDSRIHSSHV 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPSLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTAVRKSSAVVEIHQEv* 0

>NEUR_dipOrd
0 MAFNHTAGTQGQGLPHYLPEEDPFTSKLSWEADIVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASLAGQVFILNILFFCLLLPTSVIVFSYVKIIAKVKSSSKEVAHFDSRIPSSHVLEMKLTK 0
0  2
1  * 0

>NEUR_cavPor
0 MALNHTAPPQNEHLPRYLQDEDPFVSKLSWEADLVAGFYLTII 1
2 GILSTVGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 vVGKPFTIISCFRHRWVFGWIGCRWYGWAGFFFGCGSLITMTVVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYVSFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASAGGQIFILHILFFCLLLPTAMIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDSRFACCQNAGLKATKKKSLEDFR 2
1 LHTVTTDRKSAVLEIHQEV* 0

>NEUR_oryCun
0 MALNHTALPQDEHLPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRRHAYICLALIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFSCCRTSGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_ochPri
0 MALNDTALPQDEHLPHYFRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRLYGWADFFFGCGSLITMTAVSLDRYLK 1
2 GVWLKRRHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFSCCRTGGLKQTKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_canFam
0 MALNHTARPQDERLPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASLGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGRLKATKKKSLEDFR 2
1 LNTVTTVRKSSAVLEIhQEV* 0

>NEUR_felCat
0 MALNHTAPPQDERLPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAE 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_bosTau
0 MALNHTAPPPDERRPPYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTVNLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GIWLKRKHAYICLAVIWAYAAFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEVHQEv* 0

>NEUR_turTru
0  1
2 GILSTFGNGYVLYMSSRRKKKLKPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIPSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAVYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_susScr
0 MALNHTAPPPDERRPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTVNLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYAAFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLVAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIRQEV* 0

>NEUR_vicVic
0 MALNHTAPPPDERRPRHLRDGdPFASKLSWEADLVAGFYLTII 1
2 GILSTLGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWMFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2  0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHAVTTVRKSSAVLEIHQEV* 0

>NEUR_equCab
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR_myoLuc
0 MALNHTALPQDEGLPHYLQDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTVGNGYVLYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHTYICLAFIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQATVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKKVAHFDSRIH 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSSAMYNPIIYQVIDYKLACCQTGGLRATKKKSLENFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_pteVam
0 MALNHTVLPQDEHLPHYVRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTVGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTSGLRATKKKSLEDFR 2
1 LHTITTVREASAVLEIHQEV* 0

>NEUR_sorAra
0 MALNHTALPQDENLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLVVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPNSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQSGGLRATKKKSLDDFr 2
1 LHTVTTVRESSAVLEIHQEV* 0

>NEUR_eriEur
0 MSLNQTALPQDEGLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 gVWLKRKHAYLCLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASLGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKKVAHFDSRIHSSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKANKKKSLKDYR 2
1 

>NEUR_loxAfr
0 MTLNHTAPPQDDRLPQYLQDGDPFTSKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSCRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFVIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRATKKKSLEGFR 2
1 LHTVTTVKKSSAVLEVHQEv* 0

>NEUR_proCap
0 MTLNHTVLPEDDRLSHYLRDGDPFTSKLSWEADLVAGFYLTVI 1
2 GILSTCGNGYVLYMSYRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFIIISSFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2  0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSVPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCRTRGLRATKEKSLEGVR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_echTel
0 MALNHTAPPQDNSLPHYLRDGDPFVSKLSWEADLGAGFYLIII 1
2 GILSTFGNGYVLYMSYRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFSHRWVFGWTGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHMLEMKLTK 0
0  2
1 LHTITTVRKSSAVLEIHQEV* 0

>NEUR_dasNov
0 MALNHTALPQDDRLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 gILSTFGNGYVLYMSSKRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRATKKKSLEDFR 2
1 LHTVTTVRESSAVLEVHQEV* 0

>NEUR_choHof
0 MALNHTGLPQDDSLPHYFRDGDPFASKLSWEADLVAGFYLIII 1
2 GILSTFGNGYVLYMSSRRRKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLLGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCRTGGLRATKKKSFEGFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR_monDom
0 MALNHSVSPQDDYIPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKAAKKESLRTYR 2
1 LHTVTTVRRSSAVLEIHQEv* 0

>NEUR_macEug
0  1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSy 1
2 GTWLKRHHAYICLVIIWAYATFWATMPLAGLGNYAPEPFGTSCTLDWWLAQASVTGQTFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQSSHVLEMKLTK 0
0  2
1 RHTVSTIRKSSSVSETYQEV* 0

>NEUR_ornAna
0 MTNYSAPQLGDYLPHYLREGDPFVSKLSWEADLVAGVYLVII 1
2 GVLSTLGNGYVIYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIVSCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAYICLAIIWAYASFWATMPLVGLGNYAPEPFGTSCTLDWWLAQASVAGQAFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPIQFSVVPTLLAKSAAMYNPIIYQVIDCRISCCRLGGPKTGKKESLKNSR 2
1 SHSMSTIRKPSAVSGPHQEV* 0

>NEUR_galGal
0 MASDCNSSSQEEYLPHYMQQEDPFASKLSREADIIAGFYLTVI 1
2 GILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIISFFSHRWIFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLAY 1
2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCRSGGPKTLQKKSSLKESR 2
1 MYTISSHRDSAALSGTQLEV* 0

>NEUR_taeGut
0 MASEYNNSSQEEYIPHYLQEEDPFASKLSREADIIAGFYLTII 1
2 GILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIISFFSHRWMFGWIGCCWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAFICLAIIWAYAMFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQVFVLSILFFCLLLPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLLAWIPYAVVSVWSAFGRPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIECRLACCRPGG 2
1 

>NEUR_anoCar
0 MEQGQNISSQDDNQQEEDPFASKLSVEADIVAGVYLLVI 1
2 GILSTLGNGYVIYMSTQRKKKLKPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIIAFFSHRWIFGWSGCRWYGWAGFFFGIGSLITMTAVSLDRYFKICHLSY 1
2 GTWLKRHHVFICLGIIWSYAAFWATIPFAGFGNYAPEPFGTSCTLDWWLAQGSVAGQAFILNILFFCLVLPTAVIMFCYVKIIAKVQSSTKEVAHYDTRIQNQHVLEMKLTK 0
0 VAMLICAGFMFAWIPYAVVSVWSAFGRPDSVPIKVSVIPTLLAKSAAMYNPVIYQVIDCKSACCRPGNLQPLQKKNSR 2
1 

>NEUR_xenTro
0 MAGNSSYREESGYIPHYERDSDPFASKLSREADIFAGVYLMAI 1
2 GILSTLGNGYVIYMACSRKKKLRPAEIMTINLAVCDLGIS 1
2 VTGKPFAIVSCFSHRWVFGWNACRWYGWAGFFFGCGSLITLTVVSLDRYLKICHLRY 1
2 GTWLKRRHAFIALAVIWAYATLWATLPLVGVGNYAPEPFGTTCTLDWWLAQASVKGQIFVLSMLFFCLLFPTMVIVFSYAKIIAKVKSSAKEVAHFDTRNQNNHTLEIKLTK 0
0 VAMLICAGFLIAWFPYAVVSVWSAFGQPDSIPIELSVVPTMMAKSASMYNPIIYQVIDCKPACCKKDKSLQNTTSR 2
1 VYTISTFRKSTTSAR* 0

>NEUR_danRer
0 MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVI 1
2 GILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGIS 1
2 VSGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTIVSFDRYLKICHLRY 1
2 gTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSR 2
1 FYTISGSIKQRPGDEASIEI* 0

>NEUR_takRub
0 MENDTSIPSGYVPHYLLRGDPFASKLSKEADIVAAFYILVI 1
2 GVLSATGNGYVIYQTIKRKTKLKPPEFMTLNLAVFDFGIS 1
2 VTGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTIVSLDRYLKICHLRY 1
2 GTWFKRHHAFLCLVFTWLYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLAQASVSGQSFVMCMLIFCLVLPTGVIVFSYVMIiLQVKSSAQEVSHFDTQNKNKHHLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGDPDSVPISISVVPTLLAKSSAMYNPIIYQVIDCKKNCAKLSCFQAWSKRKHYKTSR 2
1 FYSISASMKKRPANEVPTEI* 0

>NEUR_tetNig
0 MENETWTHSSYVPHYLLRGDPFASRLSKEADIVAALYICII 1
2 gLMSATGNGYVLYMTFKRKTKLKPPELMTLNLAIFDFGIS 1
2 VTGKPFFIVSSLSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GAWLKRHHAFLCLASVWAYAAFWATMPLVGWGSYAPEPFGTSCTLDWWLAQASVSGQSFVMAILFFCLILPTGIIVFSYVMIIFKVKSSAKEISHFDARIRNSHDLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVISAFGEPDSVPIPVSVIPTLLAKSSAMYNPIIYQVVDVKTSCTNFSCCKALKERIHFRKSR 2
1 LYTISGSLRDPLPPKEAHIEM* 0

>NEUR_gasAcu
0 MDNETRSHPSYVPHYLLRGDPFASRLSKEADIVAAFYIFII 1
2 GVMSATGNGYVLYMTFKRKTKLKPPELMTVNLAIFDFGIS 1
2 VTGKPFFIVSSLSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GTWLKRHHAFVCLALVWAYAAFWATMPLVGWGSYAPEPFGTACTLDWWLAQASVSGQSFVMAILFFCLVLPTGIIVFSYIMIIFKVKSSAKEISHFDARIKNSHSLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGEPDSVPIPVSVIPTLLAKSSAMYNPIIYQVADLKTSCTSSSCCKALKERVLFRKAr 2
1 LYTISGSLRDTLPPKEAHIEM* 0

>NEUR_oryLat
0 MENETWTHPSYIPHYLLRGDPFASRLSKEADIIAAFYICII 1
2 gIMSATGNGYVIYMTIKRKSKLKPPELMTVNLAVFDFGIS 1
2 VTGKPFFVVSSFAHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GTWLKRQHAFLCLVFVWMYAAFWATMPLVGWGNYAPEPFGTSCTLDWWLAQASVSGQSFVVAILFFCLVLPAGIIVFSYVMIIFKVKSSAKEISNFDARIKNSHNLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGEPDSVPISVSVIPTLLAKSSAMYNPIIYQVLDLKNSCMKSSCFKGLKKPRHFRKSR 2
1 FYTISGSVKDNTTAKEAQIEM* 0

>NEUR_pimPro
0 MENTSWPHSSYVPHYLLRGDPFASRLSKEADIVAAFYILII 1
2 GIMSATGNGYVIYMTIKRKSKLKPPELMTVNLAVFDFGIS 1
2 VTGKPFFVVSSFSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GTWLKRQHIFLCLVFVWIYAAFWATMPLVGWGSYAPEPFGTSCTLDWWLAQASVSGQSFVMSILFFCLVLPAGIIVFSYVMIICKVKSSSKEVSSFDARIKNSHTLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGEPDSIPIPVSVIPTLLAKSSAMYNPIIYQLVDLKNSCSTCCAKVIRKRTHFRNSr 2
1 FYTISGSLKDTAPAKEAHIEI* 0

>NEUR_calMil
0 MTAFDNSTALYSGYWLHDSLHGDPFVSKLSWEADIISACYLIVT 1
2 GLLSTLGNGYVIYLSITQKRKLKPPEILITNLAISDFGMS 1
2 VGGQPFLIISCFSHRWIFGWVGCRWHGWAGFFFGCGSLITMTVVSLDRYLKICHLQY 1
2 GSWLQRRHVFMSLAFIWFYAAFWATMPLVGWGNYAPEPFGTSCTLDWWLARVSVSGLIFVLTILFFCLLLPIIIIVFSYIKIIAKVKSSAKEVAHFDSRIQNHHSLEMNLTK 0
0  2
1  * 0

>NEUR1_braFlo from cDNA and genome chrUn  ++  176419943 176431046 11104 FE548698 
0 MATTPADRLDGLTPAGRGATTAETHADDFASKLSREADIVIGVYLILI 1
2 GTGAILGNGRVLWLSYRCRARLRPVEMFVVSLAAADVGLSLVGHPFSAASSLMGRWSFGSAGCTW 1
2 YGFVVFFLGIASIATMALMSIMRFMIVHKRY 1
2 GQYPSRRASCVLVAAAWLYGLFWACAPLA 1
2 GWSQYHPEPYGLSCSVDWGGFSRGAGGSSFIICMLLFCTAVPVVVMVTSYAAIFALYRQAQKGVVLNLQVNATFGGKRQRTER
0 IALAVCGGFLLAWLPYAVVGLWASVAGVDAVPLALASAAPLFAKSNSLWNPIIYLGMNERFR 2

>NEUR2_braFlo from traces and genome chrUn  ++  187375671 187384042 8372 nearly identical chrUn ++ 32271780 32281075 9296
0 MATTPGLPLDGLAPTGRGVTAADTLDDDFASKLSREADIVIGVYLLLI 1
2 GTGSILGNGRVLWLSYRNWAKLRPVELFVVSLAVTDVGISVFGYPFAASSSLLGRWSFGSAGCTW 1
2 YGFTGFFFGLTSIANMALMSIMRFMIVYKGY 1
2 GPYPSRRATSGLIAAAWLYGLFWACAPLA 1
2 GWSQYHVEPFGLSCTVDWGSFSRDAGGMSFIICLLVFCVAIPVTAIMASYVAISAIYRQAKKSIAGHLQDNSAMCKKRNKLE 0
0 MALAVCGGFLLAWLPYAVVGLWSAVAGVDAVPLALASAAPLFAKSSSLWNPIIYLGMNDRFR 2
 
>NEUR_strPur Strongylocentrotus purpuratus XM_001197837 CX694910 CX690664
0 MDVNAKWWTNETLRTRDQFSDDHYTSVLSYEGDIWAGVYLMFI 1
2 SLIAFIGNISVIVISLRKREKLKPIDLLTINLAIADFLICVVSYPLPMISAFRHR 0
0 WSFGKFGCVWYGFTSFLFAVGSMATLMVIALLRYAKLCRENV 1
2 DQYQSRPFVIKVIVAIWGFAFFTTAPPLFGWS 2
1 SYVPEPYHLSCTIDFADTSPSGLSYTYFTTIVVFFMPLMIIVLCYVAIARKMIHHNRRINVGHNAGRMLLEIRLLK 0
0 TACMITMAYTISWTPYAVIAMWVTYIPVNQIPDAFRILPAFCAKTSSVYNPIIYCIFNKSFRQDLSSLICCCACQCYTITINLDINSHAQQQFRRIEERR
DEVGTYKRRPLMICSNPFAWSRDFHETWRQRRIRGIHRNCRNNVRVENINVNFRRDTDMVELNAPTPAEIHRPELNTASTRSGARTKSMATHLPALEEVPSG
APQCSALLHNTPIPRSLQGTPLPYQPQPSTSDLHDEFLNPSVVSRNMCVIVVKPNIEEELSTD* 0

 Curated set of 10 Newropsins
>NEWR2_galGal GenBank 3 garbage 5'UTR translated exons
0 MDPSFANSTFQSKITEAADIVVGTCYMVF 1
2 GICSLCGNSILLYISYKKKHLLKPAEYFIINLAISDLAMTLTLYPLAVTSSLSHR 2
1 WLYGKHICLFYAFCGLFFGICSLSTLTLLSVVCCLKICFPAY 1
2 GNRFRRKHGQILIACAWTYAAIFACSPLAHWGEYGEEPYGTACCIDWQSTNVDVMSMSYTVVLFVLCFILPCGVIVTSYSLILVTVKESRKAVEQHVSGPTRINNVQTITAK 0
0 LSIAVCIGFFAAWSPYAIIAMWAAFGSIDKIPPLAFAIPAVFAKSSTLYNPIIHLLLKPNFRSNIAKDFTVIQQLCVRCCFCVKELQTYRSTFNTGLRTFKGKNESSCNALPIMEG
CSYFPSEKGSHTFECFKSYPNCFQERLSTMGCHLQDCESLENDLQVEVTQGSRNSMKVVEQEEKSTELDNLEITLEAVPVSCTFTDL* 0

>NEWR2_anoCar Anolis carolinensis
0 MESYFANTTFHSKITEAADVIVGVFYIVF 1
2 GICSFCGNSILLYVSYKKKNLLKPAEYFMINLAISDLGMTLTLYPLAVTSSLAHR 2
1 WLFGQQVCLFYAFCGVFFGVCSLTTLTLLSIVCCLKICFPVY 1
1 GNRFRPGHGWILIACAWVYAAIFAFSPLAHWGEYGAEPYGTACCIDWRISNMKKTAMSYTTALFVFCYIIPCGIIITSYTLILITVKDSRKAVEQHALGPTRMSSVHTITAK 0
0 LSIAVCIGFFVAWSPYAIIAMWAAFGSIDMIPPLAFAVPAVFAKSSTLYNPAMYLFLKPNFRSTIAKDLTVLHRLCLKSCFCPRGMQNCSYRSALEAPLKSFKGRNESSSNSVQIVGGCS
YFPCEKCHDPFECFKNYPKCCQGRLNVMDHTPRESISVENNMQSKTKHASEKYIKVVIRGEKNTDIDNLEITLEHIPTDIKFANL* 0

>NEWR2_xenTro abundant transcripts
0 MGNKSDASAFYSSISETDDIVLGVLYSVF 1
2 GLLSLSGNSMLLLVAYRKRSILKPAEFFIVNLSISDLGMTGTLFPLAIPSLFAHR 2
1 WLFDKVTCNYYAFCGMLFGLCSLTNLTVLSSVCCLKVCYPAY 1
2 GNKFSTAHSRILLLGIWAYAGLFATAPLADWGKYGPEPYGTACCLDWEASYRERKALSYTISLFVFCYLIPSSLIFISYTLIFVTVKGARRAVQQHLSPQAKGSSIHSLIIK 0
0 LSIAVCIGFLIAWTPYAIVAMMAAFGDPTKIPSLVFALAAAFAKSSTIYNPVVYLLLKPNFLNVVTKDLTLFQTMCAVVCGWCRTPAVKTPCPHKD
LKTTSKPPSSFKKSQGVCRNCVDTFECFRNYPRCCSVGNVDAAQPMAASLVRIPPANGAPQQTVQLVVSSSRTRSGVETVEVSTEAPMSDFIKDFI* 0

>NEWR2_danRer Danio rerio acquired new intron
0 MGNVSKTALFMSTISRQHDILMGSLYSVF 1
2 FVLSLLGNGMLLFVAYRKRSSLKPAEFFVVNLSVSDLGMTLSLFPLAIPSALAHR 2
1 WLFGEITCLCYAVCGVLFGLCSLTNLTALSSVCCLKVCFPNY 1
2 GNKFSSSHACVMVIGVWCYASVFAVGPLVHWGSFGPEPYGTACCINW 2
1 YTPSHDALAMSYIISLFIFCYVVPCTIIILSYTFILVTVRGSQQAVQQHVSPQTKVTNAHALIVK 0
0 LSVAVCIGFLTAWSPYAIVAMWAAFSANEQVPPTAFALAAIMAKSSTIYNPMVYLLFKPNFRKSLSQDTQMFRHRICLSHSKASPSPGMKDQERQS
SQQCNNKDGSISTPFSSGQAESYGACHVYAEAGPHYQQISRQITARVLEGSVQSEIPVKQLTEKMQNDLL* 0

>NEWR2_tetNig Tetraodon nigroviridis gene mix
0 MGNASDTSDAFNSKISKEHDFLIGSIYSVF 1
2 CVLSLMGNCILLLVAHHKRSTLKPAEFFIVNLSISDLGMTLTLFPLAIPSSFSHR 2
1 WLFGEIACQLYATCGVLFGLCSLTNLTVLSSVCCLKVCLPNL 1
2 GRFSSKFSSSHARLLVAGVWGYASVFAVGPLVQWGHYGPEPYGTACCINWQAPNHELSSLSYIVCLFLFCYVLPCAIIILSYTCILMTVRGSRQAIQQHVSPQTKTANAHALIVK 0
0 LSVSVLSVDRWLFGEIACQLYATCGVLFGLCSLTNLTVLSSVCCLKVCLPNLGEAYQTPHREGDGAQAPRWLQEGAKADFGLLSFQAASSLPLTPGSWWR
ACGVTHQCLLWGPWCSGATTVQNLTAPPAALTGRRQTTSCPLCLTSSASSSSATCCLAPSSSSPTLAS* 0

>NEWR2_takRub Fugu rubripes
0 MGNASEASDIFLSKISKEHDILIGSIYSVF 1
2 GLLSLAGNCILLLVAYHKRSMLKPAEFFIINLSISDLGMTLTLFPLAIPSSFSHR 2
1 WLFGEITCQLYAMCGVLFGLCSLTNLTALSLVCCLKVCFPNH 1
2 GSRFSSSHARLLVVGVWCYASVFAVGPLVQWGHYGPEPYGTACCIDWRAPNHELSSLSYIVCLFFFCYVLPCATIILSYTCILMTVRGSRQAIQQHVSPQTKTANAHSLIVK 0
0 LSVAVCIGFLGAWSPYAIVAMWAAFGDATWVPPDAFAIAAILAKSSTIYNPVVYLLCKPNFRECLYKDTSTLRQRIYRGSPQSEPRERFGGTSQRHKDLSISTR
LSNGQQDSYGTCLHCADDAERGHVTTSQRTACILTGSTFTEVTVGQLSAAPADLL*

>NEWR2_gasAcu Gasterosteus aculeatus
0 MGNASDTSAVFASTISKERDILMGSLYSVF 1
2 GVLSLVGNCILLLVAYHKRSTLKPAEFFIINLSISDLGMTLSLFPLAIPSAFKHR 2
1 WLFGELTCQLYAMCGVLFGLCSLTNLTALSFVCCLKVCFPNH 1
2 GNRFSSSHARLLVVAVWGYASVFAVGPLARWGRYSPEPYGTACCIDWHAPNHELAALSYIVCLFVFCYALPCATIFLSYTFILLTVRGSRQAVQQHVSPQTKTTNTHALIVK 0
0 LSVAVCIGFLGAWTPYAVVAIWAAFGDATLVPPDAFALAAMFAKSSTIYNPVVYLLCKPNFRACLYRDTTLLRQRIYRGSPRSEPKAHFGSTSQRNKDMSVSVRSSNGQQDSYGACTENA
APCHVMTPQRTACILTESTNREVTVSRLADKPQADFL*

>NEWR2_oryLat Oryzias latipes 
0 MGNVSDTSSLFASSISREHDILMGSLYSVF 1
2 GLLSLSGNSMLLLVAYRKRSILKPAEFFIVNLSISDLGMTGTLFPLAIPSLFAHR 2
1 WLFGEITCQLYAMCGVLFGLSSLTNLTALSLVCCLKVCFPNH 1
2 GNKFSFSHARLLVAGVWCYASVFAVGPLARWGRYSAEPYGTACCIDWHAPNHELWALSYILCLFIFCYALPCTIIFLSYAFILLTVRGSRQAVQQHVSPQTKTTNAHTLIVK 0
0 LSVAVCIGFLGAWTPYAVIAMWAAFGDATQVPPTAFALAAVFAKSSTIYNPMVYLLCKPNFRECLCRDTSLLRHMIYRGSPQPQERFGSDSRRNKDITASTRFSNGQQESYGACLNCTEN
TGLCQLASPQNTACILTGSTYAEVTVQQLVDKQQPDFL* 0

>NEWR2_pimPro Pimephales promelas
0 MGNVSETALFVSTISRQHDILMGSLYSVF 1
2 CVLSLLGNGMLLFVAYRKRSSLKPAEFFVINLSVSDLGMTLSLFPLAIPSALAHR 2
1 WLFGEVVCLCYAVCGVLFGLCSLTNLTALSSVCCLKVCCPNY 1
2 GNKFSSNHACVMVIGVWCYASVFAVGPLIRWGSFAPEPYGTACCINWYIPSHDALAMSYIISLFIFCYVVPCTIIILSYTFILLRVRGSRQAVQKHVSPKTKETNAHTLIVK 0
0 LSVAVCIGFVTAWSPYAVVAMWAAFSANEPVPPTAFALAAILAKSSTIYNPMVYLLFKPNFRKILSQDTQNIRHRMCVSHSKASPTPEIK-AQSSQQCKDATISTPFSSGQAESYGTCHIYAEAEPHFQQISPQRTVRILEGIIQSEISVRHMTDRMQNDLL* 0

>NEWR2_calMil frag
0  1
2 GILSLVGNSVLLFVAYRKRQILKPAEYFVANLAVSDISMTVTLLPLAISSNFSHR 2
1 WLFVSKpCMYYGFCSMLFGICSLTNLTVLSTVCCMKVCFPAY 1
2 0
0 MSVVMIVMFLLAWSPYSIVCLWASFGNPKLIPPAMAIIAPLFAKSSTFYNPCIYVISYTMTVIAVNFVVPLSVMFFCYYNV