PRDM9: meiosis and recombination

Introduction

(to be continued)

Comparative genomics of PRDM9 and PRDM7

PRDM9 is one of many human proteins sharing a set of common domains, as well as various multiplicities of the zinc finger domain C2H2. The diagram at left shows an effort at organizing these into phylogenetic tree according to structural considerations of the SET domain these proteins all share.

The traditional SET domain is too small for an enzyme with distinctive substrates so flanking sequence must be added despite its lack of apparent conservation. Using S-adenosyl methionine, PRDM9 places the third methyl group only on the fourth position arginine in histone H3, one of many such epigenetic methylases in the human genome. The histone recognized by such methylases correlates poorly with evolutionary grouping by SET domain (figure).

The upper left corner shows the variability in domain structure. While PRDM9 and PRDM7 share the same domains (an upstream KRAB domain is not shown), of PR-class homologs, PRDM11 shares only the SET domain despite nesting deep within the PRDM9 subtree. PRDM4 has both the SET and C2H2 domains, possibly sharing the early C2H2 domain in an exon beginning with a phase 2 splice acceptor (as shown in reference sequence section). Overall however, PRDM9 and PRDM7 have no full length homologs with matching exon structure. Even the SET domain is intronated differently within PR-class proteins (with the sole exception of PRDM11), suggesting either ancient divergence or unusual evolution. These incongruities may have arisen from domain shuffling, gain and loss.

The human PRDM9 sequence below is annotated in color for domains relative to exon breaks. The protein can be best understood in terms of concatenated domains, not all of which may be present in antecedent and descendant homologs. The first two domains KRAB and SSXRD interact with transcription factors.

Each C2H2 domain -- so named for two cysteines and two histidines liganding to a structural zinc ion -- recognizes a specific trinucleotide (more or less) and so concatenated in a large array recognize specific binding sites along the genome, though tolerance of nucleotide variability and synergistic effects between adjacent units make it difficult to read out these sites precisely, despite immense efforts.

The concatenated C2H2 domains, conserved at the amino acid level so necessarily similar at the dna level, are prone to replication slippage. This process can give rise to point mutations as well as leading to a peaked distribution of repeat number rather than to a single number. Many other unrelated genes with internal repeats (such as the octapeptide region of the prion gene PRNP) are also affected by replication slippage. Such proteins regions are conveniently identified genomewide by mRNA dot plots.

The C2H2 domains generally reside in a long distinctive terminal exon of splicing phase 2 that has been shuffled over mammalian evolutionary time into various contexts. Concepts such as paralogy and orthology need piecewise definitions in these composite proteins. Synteny (gene adjacency) plays a major role in reliably deconstructing events in specific lineages.

Here the unrelated single-copy conserved gene GAS8 plays an important role. PRDM7 occurs immediately distal to it on the negative strand, making the two genes are convergently transcribed). PRDM7 is otherwise the last gene on the q arm of its chromosome in many species which may predispose it to copy number dispersal events. PRDM9 is not consistently located within placental mammals, suggesting independent relocation events.

Both PRDM9 and PRDM7 contain a seldom-mentioned C2H2 domain early in the exon annotated by SwissProt and readily found by the online domain tools regardless of species. This domain conserves the four critical residues needed for zinc binding (and so the associated fold) but lacks the terminal cap TGEKP which otherwise serves to lock down a C2H2 zinc finger after it has scanned along genomic dna to an appropriate trinucleotide. The function of this early domain and the following 112 residues are unknown -- no homologous 3D structure has ever been determined.

The first C2H2 of the main repeat region is proximaly degenerate, beginning in VKY in all species (instead of YCE). The tyrosine cannot plausibly replace the usual cysteine for zinc binding though the other three needed residues are present. This domain ends in a typical cap region TGEKP. Humans are the exception here where the conserved helix-ending proline has been replaced with leucine in the reference human genome with unknown functional consequences.

>PRDM9_homSap Homo sapiens (human) Q9NQV7 10 exons chr5:23,509,579 span 18,301 bp KRAB SSXRD SET C2H2 cap
0 MSPEKSQEESPEEDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMALRVEQRKHQK 0
0 GMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKL 1
2 ELRKKETERKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSVKSDVITHQRTHTGEKL
YVCRECGRGFSWKSHLLIHQRIHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSRQSVLLTHQRRHTGEKP
YVCRECGRGFSRQSVLLTHQRRHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSNKSHLLRHQRTHTGEKP
YVCRECGRGFRDKSHLLRHQRTHTGEKP
YVCRECGRGFRDKSNLLSHQRTHTGEKP
YVCRECGRGFSNKSHLLRHQRTHTGEKP
YVCRECGRGFRNKSHLLRHQRTHTGEKP
YVCRECGRGFSDRSSLCYHQRTHTGEKP YVCREDE* 0
          -1 23  6           traditional numbering of dna recognizing amino acids
HPCPSCCLAFSSQKFLSQHVERNH     alignment of early C2H2 domain
  *  *            *  *       zinc liganding positions

Only in PRDM11 (and PRDM1 to a lesser extent) is the SET domain intronated like PRDM9 and PRDM7:

>PRDM9_homSap 
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1

>PRDM11_homSap intronation of SET domain
2 FCESCQEYFVDECPNHGPPVFVSDTPVPVGIPDRAALTIPQGMEVVKDTSGESDVRCVNEVIPKGHIFGPYEGQISTQDKSAGFFSWL 0
0 IVDKNNRYKSIDGSDETKANWMR 2
1 YVVISREEREQNLLAFQHSERIYFRACRDIRPGEWLRVWYSEDYMKRLHSMSQETIHRNLAR 1

>PRDM1_homSap intronation of SET domain
0 AAPKCNSSTVRFQGLAEGTKGTMKMDMEDADMTLWTEAEFEEKCTYIVNDHPWDSGADGGTSVQAEASLPRNLLFKYATNSEE 0
0 VIGVMSKEYIPKGTRFGPLIGEIYTNDTVPKNANRKYFWR 0
0 IYSRGELHHFIDGFNEEKSNWMRYVNPAHSPREQNLAACQNGMNIYFYTIKPIPANQELLVWYCRDFAERLHYPYPGELTMMNL 1

>PRDM4_homSap intronation of SET domain
2 WCTLCDRAYPSDCPEHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGAEV 1
2 GVWTGETIPVRTCFGPLIGQQSHSMEVAEWTDKAVNHIWK 0
0 IYHNGVLEFCIITTDENECNWMMFVRKAR 2
1 NREEQNLVAYPHDGKIFFCTSQDIPPENELLFYYSRDYAQQI 1

Divergence of SET domains:

Gene duplication, loss and syntenic dispersion

(to be continued)

Structural considerations in C2H2 zinc fingers

High resolution structures of C2H2 zinc finger domains have been available for decades. As the name suggests, the divalent zinc atom locks the two cysteines and two histidines into a rigid geometry providing a core conformation that a small peptide of 28 residues could not otherwise stably assume. Note in the unbound state, finger tips must retain flexibility while the domain ensemble scans its genome for specific dna sequences appropriate to its function. Each finger binds a trinucleotide -- in effect making a zinc finger the protein counterpart to tRNA anticodon. However overall binding is not a simple read-off code because adjacent fingers alter each other's specificities in subtle ways.

The linker region TGEKP plays a key role when the correct DNA sequence is encountered, snap-locking its finger down onto its target by capping the C-terminus of its alpha helix. A hydrogen bond between the first threonine and middle glutamate is key to this binding-induced conformational shift. From comparative genomics, it appears that a serine in first position can also form this hydrogen bond. The role of the glycine is to stay out of the way; the lysine counterbalances the negative charge of the glutamate; the proline terminates any helical propensity, allowing a fresh start in the adjacent finger.

While this motif is immensely conserved within C2H2 zinc finger of PDRM9 homologs, exceptions do occur. It is important to understand these because these loss of dna lock-down could loosen or even eliminate trinucleotide binding specificity. Such steps might represent initial stages of pseudogenization. However many exceptions occur within the first or last fingers. It is also common for fragmentary and imperfect motifs to end the protein, sometimes continuing on in another reading frame past the current stop codon.

Note in aligning zinc finger motifs, the breaks should always be put at the end of the linker region. It is completely illogical to break at the first cysteine as some authors do because capping by the linker region is specific to its zinc finger, not the following one.

Predicting dna binding sites of zinc finger domains

Online References

Open 37 abstracts on PRDM9 and related issues. Or the reverse chronological list below provides free full text for individual articles when that is available:

abs 2011  Neaves       Unisexual reproduction among vertebrates.  Trends Genet. 2011 Mar;27(3):81-8.
abs 2011  Ponting      What are the genomic drivers of the rapid evolution of PRDM9?  Trends Genetics (2011) 1–7
htm 2011  Yanover      Extensive protein and DNA backbone sampling improves structure-based specificity prediction for C2H2 zinc fingers.  Nucleic Acids Res. 2011 Feb 22
pdf 2011  Ubeda        Red Queen theory of recombination hotspots.  J Evol Biol. 2011 Mar;24(3):541-53.
abs 2010  Hochwagen    Meiosis: a PRDM9 guide to the hotspots of recombination.  Curr Biol. 2010 Mar 23;20(6):R271-4.
abs 2010  Klug         The discovery of zinc fingers and practical applications in gene regulation and genome manipulation.  Q Rev Biophys. 2010 Feb;43(1):1-21.
abs 2010  Berg         PRDM9 variation strongly influences recombination hot-spot activity and meiotic instability in humans.  Nat Genet. 2010 Oct;42(10):859-63.
abs 2010  McVean       PRDM9 marks the spot.  Nat Genet. 2010 Oct;42(10):821-2.
pdf 2010  Kong         Fine-scale recombination rate differences between sexes, populations and individuals.  Nature. 2010 Oct 28;467(7319):1099-103.
pmc 2010  Parvanov     Prdm9 controls activation of mammalian recombination hotspots.  Science. 2010 Feb 12;327(5967):835.
pmc 2010  Lorenz       The ancient mammalian KRAB zinc finger gene cluster on human chromosome 8q24.3  BMC Genomics. 2010 Mar 26;11:206. 
pmc 2010  Neale        PRDM9 points the zinc finger at meiotic recombination hotspots.  Genome Biol. 2010;11(2):104.
pmc 2010  Sandovici    PRDM9 sticks its zinc fingers into recombination hotspots and between species.  F1000 Biol Rep. 2010 May 24;2.
pmc 2010  Billings     Patterns of recombination activity on mouse chromosome 11 revealed by high resolution mapping.  PLoS One. 2010 Dec 8;5(12):e15340.
htm 2010  Cheung       Genetic control of hotspots.  Science. 2010 Feb 12;327(5967):791-2.
pdf 2010  Urnov        Highly efficient endogenous human gene correction using designed zinc-finger nucleases.  Nature. 2005 Jun 2;435(7042):646-51.
htm 2010  Zheng        Detecting sequence polymorphisms associated with meiotic recombination hotspots in the human genome.  Genome Biol. 2010;11(10):R103.
htm 2010  Baudat       PRDM9 is a major determinant of meiotic recombination hotspots in humans and mice.  Science. 2010 Feb 12;327(5967):836-40.
htm 2010  Myers        Drive against hotspot motifs in primates implicates the PRDM9 gene in meiotic recombination.  Science. 2010 Feb 12;327(5967):876-9.
pmc 2009  Berglund     Hotspots of biased nucleotide substitutions in human genes.  PLoS Biol. 2009 Jan 27;7(1):e26.
pmc 2009  Thomas       Evolution of C2H2-zinc finger genes revisited.  BMC Evol Biol. 2009 Mar 4;9:51.
pmc 2009  Oliver       Accelerated evolution of the Prdm9 speciation gene across diverse metazoan taxa.  PLoS Genet. 2009 Dec;5(12):e1000753.
pmc 2009  Thomas       Extraordinary molecular evolution in the PRDM9 fertility gene.  PLoS One. 2009 Dec 30;4(12):e8505.
htm 2009  Willis       Origin of species in overdrive.  Science. 2009 Jan 16;323(5912):350-1.
htm 2009  Irie         Single-nucleotide polymorphisms of the PRDM9 (MEISETZ) gene in patients with nonobstructive azoospermia.  J Androl. 2009 Jul-Aug;30(4):426-31.
htm 2009  Mihola       A mouse speciation gene encodes a meiotic histone H3 methyltransferase.  Science. 2009 Jan 16;323(5912):373-5.
abs 2008  Brayer       The protein-binding potential of C2H2 zinc finger domains.  Cell Biochem Biophys. 2008;51(1):9-19.
pmc 2008  Duret        The impact of recombination on nucleotide substitutions in  the human genome.  PLoS Genet. 2008 May 9;4(5):e1000071.
pmc 2008  Miyamoto     Two single nucleotide polymorphisms in PRDM9 (MEISETZ) gene may be a genetic risk factor for Japanese patients with azoospermia by meiotic arrest.  J Assist Reprod Genet. 2008 Nov-Dec;25(11-12):553-7.
htm 2008  Cho          Prediction of DNA binding sites for zinc finger proteins.  BBRC 2008 May 9;369(3):845-8.
pmc 2007  Coop         Live hot, die young: transmission distortion in recombination hotspots.  PLoS Genet. 2007 Mar 9;3(3):e35.
pmc 2007  Fumasoni     Family expansion and gene rearrangements contributed to the functional specialization of PRDM genes in vertebrates.  BMC Evol Biol. 2007 Oct 4;7:187.
pdf 2006  Phillips     A family of zinc-finger proteins is required for chromosome-specific pairing and synapsis during meiosis.  Dev Cell. 2006 Dec;11(6):817-29.
htm 2006  Birtle       Meisetz and the birth of the KRAB motif.  Bioinformatics. 2006 Dec 1;22(23):2841-5. 
pdf 2006  Hayashi      Meisetz, a novel histone tri-methyltransferase, regulates meiosis-specific epigenesis.  Cell Cycle. 2006 Mar;5(6):615-20.
abs 2000  Laity        DNA-induced alpha-helix capping in conserved linker sequences is a determinant of binding affinity in Cys(2)-His(2) zinc fingers.  J Mol Biol. 2000 Jan 28;295(4):719-27.

Curated reference sequences

The sequences below have been compiled from genome projects -- only rarely do validating transcripts exist at GenBank. Sequences with a single frameshift or other glitch have been edited to allow full length proteins on the theory that the error either reflects an aberrant atypical individual chosen for sequencing or simple error in low coverage projects within a difficult repeat region. However such sequences may instead reflect early stages of pseudogenization. Many sequences are in fact clearly pseudogenes; here recognizable exons have been collected to allow rough dating of loss of function.

In the case of more intensively studied species such as human and mouse, the number of C2H2 repeats varies widely. Only the most common representative is shown here. This variation likely occurs in all species but the individual animal chosen for sequencing may or may not be typical. Many clades have distinctive patterns of gene amplification and gene loss, making both orthologous and functional comparisons problematic.

Other useful sequences such as the GAS8 synteny neighbor, other zinc finger quasi-homologs having similar exon and domain structures, and bogus orthologs outside of mammals are also included for reference purposes.

>PRDM9_homSap Homo sapiens (human) Q9NQV7 10 exons chr5:23,509,579 size 18,301 bp KRAB SSXRD SET C2H2
0 MSPEKSQEESPEEDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMALRVEQRKHQK 0
0 GMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKL 1
2 ELRKKETERKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSVKSDVITHQRTHTGEKL
YVCRECGRGFSWKSHLLIHQRIHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSRQSVLLTHQRRHTGEKP
YVCRECGRGFSRQSVLLTHQRRHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSNKSHLLRHQRTHTGEKP
YVCRECGRGFRDKSHLLRHQRTHTGEKP
YVCRECGRGFRDKSNLLSHQRTHTGEKP
YVCRECGRGFSNKSHLLRHQRTHTGEKP
YVCRECGRGFRNKSHLLRHQRTHTGEKP
YVCRECGRGFSDRSSLCYHQRTHTGEKP YVCREDE* 0

>PRDM9_homNea Homo sapiens (neanderthal) variants R HDL S R
0 MSPEKSQEESPEEDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMALRVEQRKHQK 1
0 GMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKL 1
2 ELRKKETERKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 1
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 2
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSVKSDVITHQRTHTGEKL
YVCRECGRGFSWKSHLLIHQRIHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSRQSVLLTHQRRHTGEKP
YVCRECGRGFSRQSVLLTHQRRHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSWQSVLLTHQRTHTGEKP
YVCRECGRGFSNKSHLLRHQRTHTGEKP
YVCRECGRGFRDKSHLLRHQRTHTGEKP
YVCRECGRGFRDKSNLLSHQRTHTGEKP
YVCRECGRGFSNKSHLLRHQRTHTGEKP
YVCRECGRGFRNKSHLLRHQRTHTGEKP
YVCRECGRGFSDRSSLCYHQRTHTGEKP

>PRDM9_panTro Pan troglodytes (chimp) frag assembly glitch *VCREDE*
0 MSPERSQEESPEEDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPLMALRVEQRKHQK 0
0 GMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPPGEASTSGQHSRLKL 1
2 ELKKKETEGKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYKGRITEDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPRSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTAKLFVGVGISRIAK
VKYGECGQGFSVKSDVITHQRTHTGEKP
YVCRECGRGFSWKSHLLSHQRTHTGEKP
YVCRECGRGFSVKSSLLSHRTTHTGEKP
YVCRECGRGFSVKSSLLSHQRTHTGEKP
YVCRECGRGFSQQSNLLSHQRTHTGEKP
YVCRECGRGFSVKSSLLSHQRTHTGEKP
YVCRECGRGFSVKSSLLSHQRTHTGEKP
YVCRECGRGFSKQSHLLSHQRTHTGEKP
YVCRECGRGFSVQSNLLSHQRTHTGEKL
YVCRECGRGFSQQSHLLRHQRTHTGEKP
YVCR   LLSHQRTHTGEKP
YVCRECGRGFSVKSSLLSHQRTHTGEKP
YVCRECGRGFSKQSHLLSHQRTHTGEKP
YVCRECGRGFSQQSHLLSHQRTHTGEKP
YVCRECGRGFSQQSHLLRHQRTHTGEKP
YVCRECGRGFSVKSSLLSHQRTHTGEKP
YVCRECGRGFSVKSSLLSHQRTHTGEKP
YVCRECERGFSQQSHLLRHQRTHTGEKP
YVCRECGRGFSRQSALLIHQRTHTGEKP* 0

>PDRM9_ponAbe Pongo abelii (orangutan) GEKPYVCRECGRGFSVKSNLLSHQRTHTEEKLYVCREDE*
0 MSPERSQEESPEDDTERTERKPT 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMALRVEQRKHQK 0
0 GMPKASFNNESSLKELSETANLLNASGSEQAQKPVSPPGEASTSGQHSRLKL 1
2 ELRSKETEGNTYSLRERKGHAYKEISEPQDDDYL 1
2 CEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALTLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITKDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNHEQQYSDPRSCNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSVKSDVITHQRTHTGEKP
YVCRECGRGFSRQSVLLIHQRTHTGEKP
YVCRECGRGFSRRSVLLIHQRTHTGEKP
YVCRECGRGFSQQSVLLIHQRTHTGEKP
YVCRECGRGFSRRSVLLIHQRTHTGEKP
YVCRECGRGFSWKSVLLRHQRTHTGEKP
YVCRECGRGFSQQSVVFIHQRTHTGEKP
YVCRECGRGFSGKSVLFRHQRTHTGEKP
YVCRECGRGFSDKSGVCYHQRTHTRGEA LCLQGVWAGL* 0      

>PRDM9_macMul Macaca mulatta (rhesus)
0 MSPERSQEESPEEDTERTERKPT 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMAVRVEQSKHQK 0
0 GMPKASFNNESSLKEVSGMANLLNTSGSEQAQKPVSPPGEARTSGQHSRLKL 1
2 ELRRKETEGKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFIKDSAVEKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITQDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSTQNFPGPSARRLFQPENLCSGDQNQEQQYSDPRSCNDKTKGQEIKERSKLLNKRTWPKEISRAFSSPPKGQMGSSRVGERMMEEEYRTGQKVNPENTGKLFVGVGISRIAK
VKYGECGQGFSDKSDVIIHQRTHTGEKP
YLCRECGRGFSQKSSLRRHQRTHTGEKP
YLCRECGRGFRDNSSLRYHQRTHTGEKP
YLCRECGRGFSNNSGLCYHQRTHTGEKP
YLCRECGRGFSDNSSLHRHQRTHTGEKP
YLCRECGRGFSNNSGLRYHQRTHTGEKP
YLCRECGRGFSNNSGLRHHQRTHTGEKP
YLCRECGRGFSQKANLLRHQRTHTGEKP
YLCRECGRGFSQKADLLSHQRTHTGEKP*

>PRDM9_calJac Callithrix jacchus (marmoset) one frameshift in repeat area chr20 terminus
0 MSPERSQEESPEGDTGRTEQKPM 0
0 VKDAFKDISMYFSKEEWAEMGDWEKTRYRNMKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPGMAFRVGQSKHQK 0
0 GMPKASFGNESSLKKLSGTANVLNTSGPEQAQKPVSPPGEASTSGQHSRLKL 1
2 ELRRKDTEEKMYSLRERKGLAYKEVSEPQDDDYL 1
2 yCEICQNFFIDSCAAHGPPTFVKDSAVDKGHPNHAALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRVTEDEEAASSGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 ESKPEIHPCPSCCLAFSSQKFLSHHVERNHSSQNFPGTSTRKLLQPENPCPGKQKEEQQYFDPCNSNDKTKGQETKERSKLLNIRTWQREMARAFSNPPKGQMGSSRVEERMMEEESRTGQKVNPVDTGKLFVGVGISRIAK
AKYGECGQGFSDMSDVTGHQRTHTGEKP
YVCRECGRGFSQKSALLSHQRTHTGEKP
YVCRECGRGFSQKSHLLSHQRTHTGEKP
YVCTECGRGFSQKSVLLSHQRTHTGEKP
YVCTECGRGFSRKSNLLSHQRTHTGEKP
YVCRECGRGFSRKSALLSHQRTHTGEKP
YVCRKCGRGFSQKSNLLSHQGTHTGEKP
YVCTECGRGFSQKSHLLSHQRTHTGEKP
YVCRKCGRGFSQKSNLLSHQRTHTGEKP
YVCRECGRGFSFKSALLRHQRTHTGEKP
YVCRECGRGFSRKSHLLSHQGTHIGEKP
YVCRECGRGFSRKSNLLSHQRIHTGEKP YVRREDE*

>PRDM9_musMus Mus musculus (mouse) Q96EQ9
0 MNTNKLEENSPEEDTGKFEWKPK 0
0 VKDEFKDISIYFSKEEWAEMGEWEKIRYRNVKRNYKMLISI 1
2 GLRAPRPAFMCYQRQAMKPQINDSEDSDEEWTPKQQ 1
2 VSPPWVPFRVKHSKQQK 0
0 ESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKL 1
2 RKKNVEVKMYRLRERKGLAYEEVSEPQDDDYL 1
2 YCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNC 1
2 YEYVDGQDESQANWMR 2
1 YVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKMKKGFTAGR 1
2 ELRTEIHPCLLCSLAFSSQKFLTQHMEWNHRTEIFPGTSARINPKPGDPCSDQLQEQHVDSQNKNDKASNEVKRKSKPRQRISTTFPSTLKEQMRSEESKRTVEELRTGQTTNTEDTVKSFIASEIS
SIERQCGQYFSDKSNVNEHQKTHTGEKP
YVCRECGRGFTQNSHLIQHQRTHTGEKP
YVCRECGRGFTQKSDLIKHQRTHTGEKP
YVCRECGRGFTQKSDLIKHQRTHTGEKP
YVCRECGRGFTQKSVLIKHQRTHTGEKP
YVCRECGRGFTQKSVLIKHQRTHTGEKP
YVCRECGRGFTAKSVLIQHQRTHTGEKP
YVCRECGRGFTAKSNLIQHQRTHTGEKP
YVCRECGRGFTAKSVLIQHQRTHTGEKP
YVCRECGRGFTAKSVLIQHQRTHTGEKP
YVCRECGRGFTQKSNLIKHQRTHTGEKP
YVCRECGWGFTQKSDLIQHQRTHTREK* 0

>PRDM9_ratNor Rattus norvegicus (rat) P0C6Y7
0 MNTNKPEENSTEGDAGKLEWKPK 0
0 VKDEFKDISIYFSKEEWAEMGEWEKIRYRNVKRNYKMLISI 1
2 GLRAPRPAFMCYQRQAIKPQINDNEDSDEEWTPKQQ 1
2 VSSPWVPFRVKHSKQQK 0
0 ETPRMPLSDKSSVKEVFGIENLLNTSGSEHAQKPVCSPEEGNTSGQHFGKKLKL 1
2 RRKNVEVNRYRLRERKDLAYEEVSEPQDDDYL 1
2 YCEKCQNFFIDSCPNHGPPVFVKDSVVDRGHPNHSVLSLPPGLRIGPSGIPEAGLGVWNEASDLPVGLHFGPYKGQITEDEEAANSGYSWLITKGRNC 1
2 YEYVDGQDESQANWMR 2
1 YVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGRELLVWYGDEYGQELGIKWGSKMKKGFTAGR 1
2 ELRTEIHPCFLCSLAFSSQKFLTQHVEWNHRTEIFPGASARINPKPGDPCPDQLQEHFDSQNKNDKASNEVKRKSKPRHKWTRQRISTAFSSTLKEQMRSEESKRTVEEELRTGQTTNIEDTAKSFIASETS
RIERQCGQCFSDKSNVSEHQRTHTGEKP
YICRECGRGFSQKSDLIKHQRTHTEEKP
YICRECGRGFTQKSDLIKHQRTHTEEKP
YICRECGRGFTQKSDLIKHQRTHTGEKP
YICRECGRGFTQKSDLIKHQRTHTEEKP
YICRECGRGFTQKSSLIRHQRTHTGEKP
YICRECGLGFTQKSNLIRHLRTHTGEKP
YICRECGLGFTRKSNLIQHQRTHTGEKP
YICRECGQGLTWKSSLIQHQRTHTGEKP
YICRECGRGFTWKSSLIQHQRTHTVEK* 0

>PRDM9_turTru Tursiops truncatus (dolphin)
0 MSTDRWPEDSTEGDAGRTAWKPT 0
0 VKDAFKDISIYFSKEEWTEMGEWEKIRYRNVKKNYEALVTL 1
2 GLRAPRPAFMCHRRQAIKAQVGDPEDSDEEWTPRQQ 1
2 VKPSWVAFRVEHSKHQK 0
0 AVPPVPLSNESSLKKLPGAAQLQKASGPAQAQSPAPPPGAASTSAWHTRQKL 1
2 ERRAKQIEVKMYSLRERKGHVYQEVSEPQDDDYL 1
2 yCEKCQNFFIDSCAAHGAPTFVKDSAVEKGHPNRSALTLPPGLSIRPSGIPEAGLGVWNEASDLPLGLHFGPYEGQITEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDTSWANWMR 2
1 YVNCARDEEEQNLVAFQYHRQIFYRTCRVVRPGCELLVWYGDEYSQELGIPWGSGWKSQLVAAGR 1
2 DPKPKIQPCGSCSLAFSSQKILSQHVECSHPSQVLPRTSARDRVQPEDPCPGYQNRQQQYSDPHSWSNKPECQEVKERSKPLLKRIRLGRISRAFSSSPKGQMGSSRAHERMMEAGPSTGQKVNPEATGKLLIGAGVSRVVK
VKYRSSGQGSKDRSSLTKHQRTHTGEKP
YVCGECGRDFSLKSDLIRHQRTHTGEKP
YVCGECGRDFSLKSGLISHQRTHTGEKP
YVCGECGRDFSQKSGLIRHQRTHTGEKP
YVCGECGRDFSLKSGLISHQRTHTGEKP
YVCGECGRDFSQKSGLIRHQRTHTGEKP
YVCGECGRDFSLKSGLITHQRTHTGEKP
YVCGECGRDFSQKSNLITHQRTHTGEKP
YVCGECGRDFSRKSSYI* 0

>PRDM9_pteVam Pteropus vampyrus (bat) pseudogene
2 LRRKGVEVKMDSLRERMGRVYQEVSEPQDDDYL 1
2 CEKCQNFFIDSCAAHGSPIFVKDSEVDIGHPNHSALTLPPGLRIGPSGIPEAGLGVWNEASNLPLGLLFGPYEGQVTEDEEAANSKYS*M 0
0 spKGETAEYVDGKDESRANWMR 2
1 YVNCARDDEDQNLVAFQFRRQIFYRTCRVIMPGCELLVWYGDEYGQGLGIKWGSKWKREFTAGR 1
2 EPKPEIHPCPSCSLAFSSRKFLSQHMKRSHPSQSLPGISARKHLQSKEPHPEDQSQQQQQQQHTDPCSWNDKAEGQEVKERSKPMLERNGQRKISRAFSKPPKGQMGSPRECERMMEAEPSTSQKVNPENTGKSSVGVGASRIVR
VKYGGCGHGFDDGSHFIRHQRTHSGEKP
FVCRECERGFNEKSSLTMHQRTHSGEKP
FVCRECE*GFSVKSSLIRHQRTYSGEKP
FVCRECEQGFNEKSSLTMHQRTHSGEKP
FFCRECE*GFSVKSSLIRHQRTHSGQKP
FVCRECKRGFTQKSHLITHQRTHSGEKP
FAGSVSEALHKSHISSSTRGHTQGRSPLFAGSVRESLALNCISTATGQM*

>PRDM9_bosTau1 Bos taurus (cattle) NW_003053109 SRSAHILVAQLLSPKVKACDGSHAHRHQQHRPPGSWPNCEVQQRPVDTL YVCRECE*
0 MRPNTSPEESTERDAGRTEWKPT 0
0 AKDAFKDISVYFSKEEWEEMGEWEKIRYRNVKRNYEALIAI 1
2 GFRATRPAFMHHRRQVIKLQADDTEDSDEEWTPRQQ 1
2 GKLSSMAFRVEHNKHQN 0
0 TMSRAPLSKEFSLKELPGAAKLLKTSGSKQAQKLVPPPGKARTPGQHPRQKV 1
2 ELRRKETEVKRYSLRERKGHVYQEVSEPQDDDYL 1
2 YCEECQSFFIDSCAAHGPPIFVKDCAVEKGHANRSALTLPPGLSIRESSIPEAGLGVWNEVSDLPLGLHFGPYEGQITDDEEAANSGYSWL 0
0 ITKRRNCYEYVDGKDTSLANWMR 2
1 YVNCARDDEEQNLVALQYHGQIFYRTCQVVRPGCELLVWYGDEYGQDLGIKRESSRKSELAGPR 1
2 ESKPKIHPCASCSLAFSSQKFLSQHVQHNHPSQTLLRPSARDYLQPEDPCPGSQNQQQRYSDPHSPSDKPEGREVKDRPQPLLKSIRLKRISRASSYSPRGQMGASGVHERITEEPSTSQKPNPEDTGKLFMGAGVSGIIK
VKYGECGQGSKDRSSLITNQRTHTGEKP
YVCGECGQSFNQKSTLITHQRTHTGEKP
YVCGECGRSFNQKSTLITHQRTHTGEKP
YVCGECGRSFSQKSTLIKHQRTHTGEKP
YVCGECGQSFNQKSTLITHQRTHTGEKP
YVCGECGQSFNQKSTLITHQRTHTGEKP
YVCGECGRSFSRKSTLITHQRTHRGEKL CLQGV* 0

>PRDM9_bosTauX1 Bos taurus (cattle) chrX
0 MSPNRSPENSTEGDAGRTEWKPM 0
0 AKDAFKDISIYFTKEEWAEMGEWEKIQYRNVKRNYEALIAI 1
2 GFRATQPGFMHHGRQVLKSQVDDTEDSDEEWTPRQQ 1
2 GKPSGMAFRGEPSKHPK 0
0 RLSRGPLNKVSSLKKLPGAAKLLKKSGSKQAQKPVPPPREARTPGKHPRHKV 1
2 ELRRKETEVKRYSVRERKGHVYQEVSEPQDDDYL 1
2 YCEECQNFFIDSCAAHGPPTFVKDSAVEKGHANRSALTLPPGLSIRPSGIPEAGLGVWNEASDLPLGLHFGPYEGQIIYNEEDSNSGYCWL 0
0 VTKGRNSYEYVDGKDTSLANWMR 2
1 YVNCARDDEEQNLVALQYHGQIFYRTCRVVRPGCELLVWYGDEYGEELGIKQDKRGKSKLSAQR 1
2 EPKPKIYPCASCCLSFSSQKFLSQHVQRNHPSQILLRPSIGDHLQPEDPCPGSQNQQQRYSDPHSLSDKPEGREPKERPHPLLKGPKLCIRPKRISTASSYPPKGQMGGSEVHERMTEEPSTSQKLNPEDTGKLFMEAGVSGIVR
VNYGDHEQGSKDRSSLITHEKIHTGEKP
YVCKECGKSFNGRSDLTKHKRTHTGEKP
YACGECGRSFSFKKNLITHKRTHTREKP
YVCRECGRSFNEKSRLTIHKRTHTGEKP
YVCGDCGQSFSLKSVLITHQRTHTGEKP
YVCGECGRSFNEKSRLTIHKRTHTGEKP
YVCGDCGQSFSLKSVLITHQRTHTGEKP
YVCGECGQSFNEKSRLTIHKRTHTGEKP
YACGDCGQSFSLKSVLITHQRTHTGEKP YVCMECE* 0

>PRDM9_bosTauX2 Bos taurus (cattle) chrX 93% 81%
0 MSPNRSPENSTEGDAGRTEWKPM 0
0 AKDAFKDISIYFTKEEWAEMGEWEKIRYRNVKRNYEALIAI 1
2 GFRATQPGFMHHRRQVLKPQVDDTEDSDEEWTPRQQ 1
2 GKPSGMAFRGERSKHQK 0
0 RLSRGPLNKVSSLKKLPGAAKLLKKSGSKQAQKPVPPPREARTPGKHPRHKV 1
2 ELRRKETKVKRYSVRERKGHVYQEVSEPQDDDYL 1
2 YCEECQNFFIDSCAAHGPPTFVKDSAVEKGHANRSALTLPPGLSIRPSGIPEAGLGVWNEASDLPLGLHFGPYEGQIIYNEEDSHSGYCWL 0
0 VTKGRNSYEYVDGKDTSLANWMR 2
1 YVNCARDDEEQNLVALQYHGQIFYRTCRVVRPGCELLVWYGDEYGEELGIKQDKRGKSKLSAQR 1
2 EPKPKIYPCASCCLSFSSQKFLSQHVQRNHPSQILLRPSIGDHLQPEDPCPGSQNEQQRYSDPHSLSDKPEGREPKERPHPLLKGPKLCIRLKRISTASSYPPKGQMGGSEVHERMTEEPSTSQKLNPEDTGKLFMEAGVSGIVR
VKYGEHEQDSKDKSSLITHEKIHTGEKP
YVCTECGKSFNWKSDLTKHKRTHSEEKP
YACGECGRSFSFKKNLIIHQRTHTGEKP
YVCGECGRSFSEKSNLTKHKRTHTGEKP
YACGECGQSFSFKKNLITHQRTHTGEKP
YVCGECGRSFSEKSRLTTHKRTHTGEKP
YVCGDCGQSFSLKSVLITHQRTHTGEKP
YVCRECGRSFSVISNLIRHQRTHTGEKP
YVCRECEQSFREKSNLVRHQRTHTGEKP YVCMECE* 0

>PRDM9_bosGru1 Bos grunniens (yak) transcript EF432551 99%
2         NRSALTLPPGLSIRESSIPEAGLGVWNEVSDLPLGLHFGPYEGQITDDEEAANSGYSWL 0
0 ITKRRNCYEYVDGKDTSLANWMR 2
1 YVNCARDDEEQNLVALQYHGQIFYRTCQVVRPGCELLVWYGDEYGQDLGIKRESSRKSELAAPR
2 ESKPKIHPCASCSLAFSSQKFLSQHVQHNHPSQTLLRPSARDYLQPEDPCPGSQNQQPRYSDPHSPSDKPEGREVK

>PRDM9_oviAriX1 Ovis aries (sheep)
0 MSPNRSPENSTEGDAGRTEWKPM 0
0 AKDAFKDISIYFTKEEWAEMGEWEKIRYRNVKRNYEALIAI 1
2 GFRATQPAFMHHHRQVIKPQVDDTEDSEEEWTPRQQ 1
2 GKPSGMAFRGERSKHQK 0
0 RLSRGPLNKVSSLKKLPGAAKLLKKTGSKQAQKPVPPPREARTPGQHPRHKV 1
2 ELRRKETEVKRYSLRERKGHVYQEVSELQDDDYL 1
2 CEECQNFFIDSCAAHGPPTFVKDSAVEKGHANRSALTLPPGLSIRPSGIPEAGLGVWNEASDLPLGLHFGPYEGQVIYNEEASHSGYSWL 0
0 VTKGRNSYEYVDGKDTSLANWMR 2
1 YVNCARDDEEQNLVALQYHGQIFYRTCQVVRPGCELLVWYGDEYGEELGIKQDSRGKSKLSAQR 1
2 EPKPKIHPCASCSLSFSSQKFLSQHVQRSHPSQILLRPSPRDHLQPEDPCPGKQNQQQRYSDPHSPSDKPEGQEPKERPHPLLKGPKLCIRLKRISTASSYTPKGQMGGSEVHEKMTEEPSTSQKLNPENTGKLFMEAGVSGIVR
VKYGEHEQGSKDKSSLITHERIHTGEKP
YVCKECGKSFNGRSNLTRHKRTHTGEKP
YVCRECGQSFSLKSILITHQRTHTGEKP
YVCGECGQSFSEKSNLTRHKRTHTGEKP
YVCRECGQSFSLKSILITHQRTHTGEKP
YVCRECGRSFSVKSNLTRHKMTHTGEKP
YVCGECGQSFSQKPHLIKHQRTHTGEKP
YVCRECGRSFSAMSNLIRHQRTHTGEKP
YVCRECGRSFSAMSNLIRHQRTHTGEKP YVCREC* 0

>PRDM9_oviAriX2 pseudogene
0 MSPNRSPENSTEGDAGRTEWKPM 0
0 AKDAFKDISIYFTKEEWAEMGEWEKIRYRNVKRNYEALIAI 1
2 GFRATQPAFMHHHRQVIKPQVDDTEDSEEEWTPRQQ 1
2 GKPSGMAFRGERSKHQK 0
0 GMSRGPLSKVSSLKKLPGTTKLLKTSGSKQAQKPVPSSREARTSG*HTRQKV 1
2 ELGRKETDMKRYSLRERKGHVYQEVSEPQDDDYL 1
2 CQECQNFFINSCDAHGPPTFVKDSAVEKGHANRSALTLPPGLSIRLSGIPEAGLGVWNEASHLPLGLHFGPYEGQITDDKEAVNSGYSWL 2
1 YVNCARHYEEQNLVAFQYHGQIFYRTCQVVRPGCELLVWYGDEYGEKLGIRCESRGKSMLAAGR 1
2 EPKPKIYPCASCCLSFSSQKFLSQHVQRNHPSQILLRPSIGDHLQPEDPCPGSQNEQQ*YSDPHSPSDKPEGCKAKERPPWLLKSMSV-----RISMASSYSPKGQMRGSETHYRMTEEPSTSQKLNPEDIGKLFMGTGVSGIIK
IKYEECGQVSKDRSSLITHEGTHTREQ

>PRDM9_sorAra Sorex araneus (shrew) AALT01000095
0 MSLNRPAEMNTQGKARKLMLKPM 0
0 SKDAFKDISMYFSKEEWAEMGDWEKIRHRNVKRNYEELISI 1
2 GLRAARPAFMSHRRQAIKTQLDDTEESDEEWTPNQQ 1
2 VKSLRVAFRAEQSKHQK 0
0 GRSRTPISNESSSKELSGTRTLLNTKCTKQAQKPLFPPGEASTSGHYSKPKL 1
2 ELRRKEPEVKMYSLRERKGRAYQEVSEPQDDDYL 1
2 YCENCQNFFINKCSAHGSPIFVKDNAVAKGHSNRSALTLPHGLRIGPSGIPEAGLGIWNEASDLPLGLHFGPYEGQITNDEEAANSGYSWL 0
0 ITKGRNCYEYVDGVDESLANWMR 2
1 YVNCARDYEEQNLVAFQYHRQIFYRTCRIIKPGCELLVWYGDEYGQELGIKWGSKWKSELTADK 1
2 EPKPEIYPCPCCSLAFSNQKFLSRHVEHSHPSLILPGTSARTHPKSVNFCPGDQNQWQQHSDACNDKPDEPWNDKLENHKSKGRSKPLPKRMGQKRISTAFPNLRSSKMGSSNKHETIMDKINTGQKENPKDTYRVFAGIGMPRIIR
DKHVTLRRSFTNRSSPLTHQRTHTGEKP
YVCRECGRGFSQKSHLLTHQRTHTGEKP
YVCRECGRGFTDRSSLLTHQRTHTGEKP
YVCRECGRGFSLKSSLLRHQRTHTGEKP
YVCRECGRGFSLKSSLLTHQRTHTGEKP
YVCRECGRGFTDRSSLLTHQRTHTGEKP
YVCRECGRGFSLKSSLLTHQRTHTGEKP
YVCRECGRGFSRKSSLLRHQRTHTGEKPYVCES* 0

>PRDM9_loxAfr Loxodonta africana (elephant)
0 MSPARAAKKNPRGDVGSAGRTPT 0
0 aKDTFRDISIYFSKEEWAEMGEWEKFRYRNVKRNYEALVTI 1
2 GLRAPRPAFMCHRRQAIKAQVDNTEDSDEEWTPRQQ 1
2 VKPPSVASRAEQSRHQK 0
0 GTPKALLGNESSLKEVSGTAILLNTTGSEQAQKPVSSPGEASTSDQPSRWKL 1
2 EPRRNEVEVKMYNLRERKGLEYQEVSEPQDDDYL 1
2 yCEKCQNFFIDTCAVHGAPMFVKDSPVDRGHPNHSALTLPPGLRIGPSSIPKAGLGVWNEASELPLGLHFGPYEGQVTEDKEAANSGYSWL 0
0 ITKGKNCYEYVDGKDESWANWMR 2
1 YVNCARDEEEQNLVAFQYHRQIFYRTCRTIQPDCELLVWYGDEYGQELGIKWGSRWKKELTSGR 1
2 EPKPEIHPCPSCRLAFSSQKFLSQHMKHSHPSPPFPGTPERKYLQPEDPRPGGRRQQRSEQHMWSDKAEDPEAGDGSRLVFERTRRGCISKACSSLPKGQIGSSREGNRMMETKPSPGQKANPEDAEKLFLGVGTSRIAK
VRCGECGQGFSQKSVLIRHQKTHSGEKP
YVCGECGRGFSVKSVLIKHQRTHSGEKP
YVCGECGRGFSVKSVLITHQRTHSGEKP
YVCGECGRGFSVKSVLITHQRTHSGEKP
YVCGECGRGFSQKSDLIKHQRTHSGEKP
YSCRECGRGFSRKSVLITHQRTHSGEKP
YVCGECGRGFSQKSNLITHQRTHSGEKP
YVCGECGRGFSRKSVLITHQRTHSGEKP
YVCGECGRGFSQKSNLITHQRTHSGEKP
YVCGECGRGFSQKSDLITHQRTHSGEKP
YVCRECGRGFSRKSNLITHQRTHSGEKP
YVCRECRRGFSVKSALIGHGRRKCSKSAEPLHFPRVSRDQK* 0

>PRDM9_macEug Macropus eugenii (wallaby) fragment
2 gFSAPRPTFMCHGKQNKEAKVEESGDFDEEWIRKQP 1
2 CEECQTFFLETCAVHGPPKFVQDSVMVKGHPYCSAITLPPGLRIGLSGIPGAGLGIWNEASNLPLGLHFGPYEGQMTEDDEAANSGYSWM 0
1 YVNCARDEEEQNLVAFQYHRKIFYRTCQIIRPGCELLVWYGDEYGQELGIKWGSKWKRPPITLT 1

>PRDM9_monDom Monodelphis domestica (opossum) not at GAS8 weak C2H2 domain fragment
0 GEDAFKDISTYFSKKQWVKLKEWEKVRLKNVKRNYEAMIKI 1
2 GLSVPRPAFMCRGRQNKKVKVEESGDSDEEWIPKQL 1
2 DCRRKDVEVHIYSLRERKYQVYQEMWDPQDDDYL 1
2 yCEECQIFFLDSCPLHGPPTFVQDSAMVKGHPYCSAITLPPGLRIGLSGIPGAGLGVWNEASTLPLGLHFGPYKGKMTEDDEAANSGYSWM 0
0 ITKGRNCYEYVDGKEESCSNWMR 2
1 YVNCARDEEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKRPLPELTGE 1
2 EARNLSLPFHPLGFSTHTFLNQHTLLKTAPLSVLGFRDRKIMSWEIYSRTSYLQPVPTVK
KECE*GFTHQTNLVTHRWTHSGERPYVCV*

>PRDM9a_ornAna Ornithorhynchus anatinus (platypus) fragment
RIGKKPQVRDFNLRKQKRKIYNENYRPEDDDYL
CEICQTFFLEKCVLHGPPVFVQDLPVEKWRPNRSTITLPPGMQIKVSGIPNAGLGVWNQATSLPRGLHFGPYMGIRTKNEKESHSGYSWMI
IVRGKNYEYLDGKDKAFSNWMR
YVNCARSEREQNLVAIQYQGEIYYRTCRVIPPGQELLVWYGLEYGRHLG
EARPSRYRDVVFSSGVYGSSVTLLTSGVP

>PRDM9b_ornAna Ornithorhynchus anatinus (platypus) tandem fragment
RSGKKPQVRDFNLRKQKRKMYTEESEPEDDDYL
CEDCQTFFLEKCSVHGPPVFVQDCEAKRCQQNRSEVTLPPGLLIKMSGIPNAGLGVWNQATSLPRGLYFGPFVGIRKNNVKDSLSGYSWAV
ILRGRNYEYLDGKNTSFSNWMR
ASDSWTGRERLILLPSWGVGGAASGGKRVREDSGKGGWRESR
KPVSALTTSWDSDGWERAADEVTVSLLPGERPHSSGGSFAPSARSGGVKQRIWSKRRSAA
LQRTRERRNSTHDFPPKHEDTAARQDERQCPDRGRAKQRGVRKSEQIERAKAMGRKKALG
GLSPPRRERLSDEAGQRKKSGHEQFWQKPGPSEAWAGPAEGSTIPRR
HCCDVCGKAFNRLSRLKQHKRVHTGEKP
LVCKICKRAFSDPSNLNRHAKRHTGEKP
FVCRVCGRSFNRSDNMNEHRWKHTSNNIIP NTGHMSATVVENASLCINRNYQIYKERATYL-
HCCDVCRKAFKRLSHLRQHKRIHTGEKP
LVCKVCRRTFSDPSNLNRHSRIHTGLRP
YVCKLCRKAFADPSNLKRHVFSHTGHKP
FVCEKCGKGFNRCDNLKDHSAKHSEDNSTPKP*

>PRDM7_homSap Homo sapiens (human) chr16:90,122,976 TUBB3+ DEFB+ AFG3L1+ DBNDD1- GAS8+ PRDM7- 92% identical
0 MSPERSQEESPEGDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKMNYNALITV 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMAFRGEQSKHQK 0
0 GMPKASFNNESSLRELSGTPNLLNTSDSEQAQKPVSPPGEASTSGQHSRLKL 1
2 ELRRKETEGKMYSLRERKGHAYKEISEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDKSSANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQERQYSDPRCCNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSSRVGERMMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSDKSDVITHQRTHTGGKP
YVCRECGR FSRKSDLLSHQRTHTGEKP
YVCRECERGFSRKSVLLIHQRTHRGDAP VCRKDE*

>PRDM7_panTro Pan troglodytes (chimp) pseudogene GAS8
0 MSPERSQEESPEGDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWgKTRYRiVKMNYNALITi 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMAFRGEQSKHQK 0
0 GMPKASFNNESSLkELSGmPNLLNTSgSEQAQKPVSPPGEASTSGQHSRLKL 1
2 ELRRKETvGKMYSLRERKGHAYKEISEPQDDDYL 1
2 yCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLRVWNEASDPPLGLHSGPYEGQITEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDKSwANWMR 2
1 YENCARDDEEQNLVSFQYHRQS*FYRTCRVIRPGCELLVWYGDE*GQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLQPENP*PGDQNQERQYSDPRCCNDKTKGQEVKERSKLLNKWTWQREISRAFSSLPKGQMGSSRVGERMMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSDKSDVITHQRTHTGGKP
YVCRECGQGFSRKSVLLIHQRTHRGEKP* 0 VCRKDE

>PRDM7_ponAbe Pongo abelii (orangutan) GAS*
0 MSPERSQEESPkGDTERTERKPM 0
0 VKDAFKDISIYFTKEEWTEMGDWEKTRYRNVKRNYKTLITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMAFRGEQSKHQK 0
0 GMPKASFNNESSLKELSGTQNLLNTSGSEQAQKPVSPPGEASTSGQHSTLKI 1
2 ELRRKETEGKTYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDKEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCAWDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMPGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARHLLQAENPCPGDQNQEQQYSDPDCCNDKTKGQEIKERSKLLNKRTWQREISRAFSSSAKGQMGSSRVGERMMEEESGTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSDKSDVITHQRTHTGGRS
YICRESGRGFTQKSGLLSHQRTHTGEKP
YVCRECGWGFSQKSNLLRHQRTHTGEKP
YVCRECGRGFSRKSVLLIHQRTHTGEKP* VCRKDE*

>PRDM7_nomLeu Nomascus leucogenys (gibbon) ADFV01125891 no info GAS8 pseudogene
0 MSPERSQEESPkGDTERTERKPM 0
0 VKDAFKDISIYFTKEEWTEMGDWEKTRYRNVKRNYKTLITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 IKSPWMAVRVEQSKHQK 0
0 GMPKASFNNESGLKELSGTQNLLNTSG*EQARKPVSPPGEASTSGQHSRQKL 1
2 ELRRKETEGKMYSL*ERKGHAYKEVSEPQDDDYL 1
2 yCEMCQNFFTDSCAAHGPPTFVKDSAVDKGHPNHSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKS*ANWMK 2
1 YVNCARDHEEQNLVAFQYHRQIFYRTCQVIRPGCEPLVWYGDEYGQELGIKWGSKWKKELTAER 1
2 EPKPEIHPCPSCCLVFTSQKFLSQHVECNHSSQNFPGPSARKLLQRENPCPGDQNQEQQYSDSRSCNDKTKGQEIKERSKL*NKRIWQRKISRAFSSLPKGQMGSSRVGERMMEEESRTGQKVNPGNTGKLFVGVGISRIAK
VKYGECGQGFSDKSDVIAHQGTHTGGKS
*ICRECGWGFSQESHLLIHQRTHTGEKL
YVCRECGQGFSQKSDLLSHQRTHTGEKP
YVRRECGRGFSQKSNLLSHQRTHTEEKP
YVCRECGWGFSQKSHLLIHQRTHTGKKP* VCRKDE

>PRDM7_macMul Macaca mulatta (rhesus) pseudogene
0 MSPERSQEESPEEDTERTERKPT 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMAFRVEQSKHQK 0
0 EMPKTSFNNESSLKELSGTPNLLSTSDSE*AQKPASPPGEASTSGQHSRLKL 1
2 ELRRKETEGKMYSLRERKRHAYKEASELQHDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFVKDNAVNKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPCEGRITEDKEAANSGYSWL 0
0 ITKGRNCYEYVDGKDKSWAKWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIYPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQSENPCPGDQNQEQQYSDPSSCNDKTKGQEIKERSKLLNKRTWQREILRAFTSPPKGQMGSSRVGERMMEEEFRTGQKANPGNTGKLFVGVEISRIAK
VKYGECGQGFSGKSDVITHQRTHTEGKP
YVCRGCGRRFSQKSSLLRHQRTHTGEKP*

>PRDM7_papHam Papio hamadryas (baboon) GAS* end of scaffold
0 MSPERSQEESPEEDTERTEWKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMAVRVEQSKHQK 0
0 GMPKASFNNESSLKEVSGMANLLNTSGSEQAQKPVSPPGEARTSGQHSRLKL 1
2 ELRRKETEGKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEMCQNFFIDSCAAHGPPTFIKDSAVEKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITQDEEAANNGYSWL 0
0 ITKGRNCYEYVDGKDKSWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGR 1
2 EPKPEIHPCPSCCLAFSSQKFLSQHVERNHSTQNFPGPSARRLLQPENLCSGDQNQEQQYSDPCSCNDKTKGQEIKERSKLLNKRTWQKEISRAFSSPPKGQMGSSRVGERMMEEESRTGQKVNPENIGKLFVEVGISRIAK
VKYGECGQGFSDKSDVVIHQRTHTREKP
YLCRECGRGFSQKSNLLRHQRTHTGEKP
YLCRECGRGFRDNSSLRCHQRTHTGEKP
YLCRECGRGFRDNSSLRCHQRTHTGEKP
YLCRECGRGFSDNSSLRYHQRTHTGEKP
YLCRECGRGFRDNSSLRYHQRTHTGEKP
YLCRECGRGFSVKSNLLSHQRTHTGEKP
YVCRECGRGFSDNSSLRCHQRTHTGEKP
YLCRECGRGFSQMSHLRCHQRTHTGEKP
YLCRECGRGFSVKSNLLSHQRTHTGEKP
YVCRECGRGFSRKANLLSHQRTHTGEKP* 0

>PRDM7_micMur Microcebus murinus (lemur) ABDC01433247
0 MSPEKSQEESPEEDTERTERKPM 0
0 vKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKPPWMALRVEQRKHQK 0
0 GMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKL 1
2 ELRKKETERKMYSLRERKGHAYKEVSEPQDDDYL 1
2 YCEKCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLKIRPSGIPQAGLGVWNEASELPLGLHFGPYEGQVTEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDDSWANWMR 2
1 YVNCARDEEEQNLVAFQYHRQIFYRTCQVIRPGCELLVWYGDEYGQELGIKWGSKWKEELTIRQ 1
2 EPKPEIHPCPSCSLAFSSQKFLSQHVKHTHSSQISPRTSGRKHLQPENPCPGDQNQEQQHSDPHSCNDKAKDQEVKERPKPFHKKTQQRGISRAFSSPPKGKMGSCREGKRIMEEEPRTGQKVGPGDTDKLCAAGGISRISR
VKYGDSGQSFSDKSNVIIHQRTHTGEKP
YVCRECGRGFSQKSDLLKHQRTHTGEKP
YVCRECGRGFSQKSHLLRHQRTHTGEKP
YVCRECGRGFSQKSDLLIHQRTHTGEKP
YVCRECGRGFSCKSHLLIHQRTHTGEKP
YVCRECGRGFSCKSSLLIHQRTHTGEKP
YVCRGVWGEALAESQTSSYTRGHTQGRSP
VFAGRVSKSLALNYISTATGGHLLTSHLP TPALGGASKGSLLTLYISQECKETRNN*

>PRDM7_otoGar Otolemur garnettii (galago) GAS8
0 MSPEKSQEESPEEDTERTERKPM 0
0 VKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITI 1
2 GLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQ 1
2 VKHPWMAFRMEQSKRQK 0
0 ILKKCMLSFNMHLKELSGPASLPNISGSEQHQKHMSSPREASTSGQHSGRKS 1
2 DLRIKEIEVRMYSLRERKGHAYKEVSEPQDDDYL 1
2 yCEKCQNFFIDNCAVHGPPTFVKDTAVEKGHPNRSVLSLPSGLGIRTSGIPQAGFGVWNEASDLQLGLHFGPYEGQVTEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDESQGNWMR 2
1 YVNCARDEEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELTAGQ 1
2 EPKPEIHPCPSCSLAFSTQKFLSQHVERTHPSQISQGTSGRKNLRPQTPCPRDENQEQQHSDPNSRNDKTKGQEVKEMSKTSHKKTQQSRISRIFSCPPKGQMGSSREGERMIEEEPRPDQKVGPGDTEKFCVAIGISGIVK
VKNRECVQSFSNKS NLRHQRTHTGEKP
YMCRDCGRGFSHKSSLFRHQRTHTGEKP
YVCRDCGRGFSLKANLLTHQRTHTGEKP
YVCRDCGQGFSQKAHLLRHQRTHTGEKP
YMCRDCGQGFSRKAYLLTHQRTHTGEKP
YVCRDCGQGFSQKAHLLTHQRTHTGEKP
YVCRDCGRGFSHKSSLFRHQRTHTGEKP YICRDCG*

>PRDM7_bosTau chr Un.004.251 bad XM_598831 + transcript GO353654 no zinc downstream
0 MSQNRSPEERTKGDAGRTEWKLT 0
0 AKDAFKDISIYFSKEEWAEMGEWEKTGYRNVKRNYEVLIAI 1
2 GLRATQPAFMHHRRQVIKPQGDDTEDSDEEWTPQHQ 1
2 GKPSRKAFRMEHRKHQK 0
0 GKSRGPLSKVSSLKKLQGAAKLLNTSGSKWAQKPANPPRETRTLEQHSRQKV 1
2 ELRRKETDMKRYSLRERKGHVYQEVSEPQDDDYL 1
2 YCQECQNFFIDSCDAHGPPTFVKDSAVEKGHANRSVLTLPPGLSIKLSGIPEAGLGVWNEASHLPLGLHFGPYEGQITDDKEAINSGYSWL 0
0 ITKGRNSYEYVDGKDTSLNWMR 2
1 YVNCARHYEEQNLVAFQYHGQIFYRTCQVVRPGCELLVWYGDEYGEKLGIKCESRGKSMFAAGGV 1
2 EGHPSSSTPPHSGELPR* 0

>PRDM7_bosTau Bos taurus (cow) adjacent to GAS8 and in correct orientation pseudogene but missing C2H2.
0 MSPNRSPEESIEGDTGRTEWKPT 0
0 AKDAFKDISIYFCKEEWAQMG*WEKIRYRNVKRNYEALITL 1

>PRDM7_ailMel Ailuropoda melanoleuca (panda) GAS8 synteny no 9
0 MSLNTSPEETPERDSGRTGWKPT 0
0 AKDAFKDISIYFSKEEWTEMGDWEKIRYRNVKRNYEALITI 1
2 GLRAPRPAFMCHRRQAIKPQVDDTEDSDEEWTPRRQ 1
2 VRPSWVAFRMEQSKHQR 0
0 GIPRAPLRNESSLKELSETAKLLNTSGSELGQKPVSLPGEASTSGHDSLQKL 1
2 GFRRKDVEVKMYSLRERKSLAYQEVSEPQDDDYL 1
2 yCEKCQNFFIDSCAVHGPPTFVKDSAVDKGQPNRSALTLPPGLRIRPSGIPQAGLGVWNEASDLPLGLHFGPYEGQITEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDNSWANWMR 2
1 YVNCARDEEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKSELAAGK 1
2 EPKPEIHPCPSCSLAFSSQKFLSQHLEHNHPSQILSRKSASEHFQQEDPCPGHQNQQQQQHSDPHRWNDKAKGQEVKERFKPLLKSIRQRRISRAFSSPCKGQTRSSTVCEGMVEEEPSAGQKLNPEETGKLFMGVGMSGIIR
VKYRGCGRDFSDRSHQSGHQRRHQKKP
SVCKKVKREFSHKSVLITHQRTHTGEKP
YVCRECGRGFTQRSNLIRHQRTHTGEKP
YVCRECGRGFTQRSNLIRHQRTHTGEKP
YVCRECGRGFTQRSSLIRHQRTHTGEKP
YVCRECGRGFTLRPNLIGHQRTHTEALP INYISTTKEQM* 0

>PRDM7_felCat fragment no GAS8 chrUn_ACBE01450406:1-11,793
0 MEPSPASESARGQPGGPGTTSPLRFPEQSAERGSRKARWKPT 0
0 AKDAFKDISIYFSKEEWTEMGDWEKIRYRNVKRNYEALMTI 1
2 gLRAPRPAFMCHRRQAIKPQVDVTEDSDEEWTPRQQ 1
2 VKPSWVASRVDQNKQHKV 0
0 GTHRVPLSKESSLKDFSETAKLLNTSGSEQGQKPVSLPGEASTSGHHSRRKL 1
2 frRRKEIGVKMYSLRERKGFAYQEVSEPQDDDYL 1
2 yCEKCQNFFIDSCAVHGPPTFVKDNAVGKGHPNRSALTLPPGLRIRPSSIPEAGLGVWNEASDLPLGTHFGPYEGQITEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDNSWANWMR 2

>PRDM7_canFam pseudgene
2 VKPSWVAFRMEQSKHQK 0
2 yCEK*QTFFIDSCTVHGPPTFVKDSEVDKGQPNHSALTLPPGLRIRTSSIPQAGLGVWN*ASDLPLGLHFGPYKGQITEDEEAANSGYSCL 0
0 ITKGRNCYEYVDGKDKDNSWANWMR 2
1 YMNCARDDEEQS*WPFNITGR--YSTEPVEQPGHQASCELLVWYGDEYSQELGIKWGSKWKSELTAGK 1
2 EPNPEIHPCPSCSL AFSSQKFLSQHLEHNHPSQILPRISVREHFRPKDPCPGCQNQQQQQHSDPQRWNDRAKGQEGKERFKPLPKSIRQRRISRAFSTPCKGQTTCEGIVKEEPSAGSQKLNPEDTGKLFKGVGMTRIIR
VKYRGCGRGFNDRSHLSRHQRTHTGENP
YVCRECGRGFIHRTNLIIHQRTHTGEKP
YVCRECGQALYRGQISAYIRGHTQGRSPM

>PRDM7_equCab missing front exons, some frameshifts
2 VKPSWVAFRVEQSKQQK 0
2 SGTAKLLKTSSSEQVQKPVSPLGEASSSEQHSRRKLELRRKEVGVKMYSLRERKGHAYQEVSEPQDDDYL 1
2 yCENCQNFFIDSCAAHGPPIFVKDSAVDKGHPNRSALTLPLGLRIRPSGIPEAGLGVWNEASDLPLGLHFGPYEGQITEDEEAANSGYSWL 0
0 ITKGRNCYEYVDGKDISWANWMR 2
1 YVNCARDDEEQNLVAFQYHRQIFYRTCRVVRPGCELLVWYGDEYGQELGIKWGSKWKRELTAGR 1
2 EPKLEIHPCPSCSLAFSSQKFLSQHVERNHPSQILPGTSARNHLQPEDPSPGDQNQQQQHSDPHSWKDKAHSQEVKERSKPLLKKIRQRRIPRAFSYPPKGQMENFRMRERIMEEKPSIGRKVNPEDTGKLFLEMRMSRNVR
VQYGGCGRGFNDRASLIKHQRTHTGEKP
YVCRECEQGFTQKSSLIAHQRTHTGEKP
YVCRECEQGFSEKSHLIRHQRTHTGEKP YVCRECE*

>PRDM7_loxAfr (elephant) pseudogene adjacent to GAS8 in standard orientation many frameshifts
0 MSLNTSPEETPERDSGRTGWKPT 0
0 AKDAFRDIFIYFSKEGYVEMGEWEKLCYRNLKMNYKALVTT 1
2 GLRASHPAFTCHCMQAIKAQMDDTEDSNEEQTPRQ 1
2 VRPSWVAFRMEQSKHQR 0
0 GMLRVPRSNESSLKNLSGTSIMLSRAGSEQAQKLVLPPGKASTSDEHSRQKP 1
2 EHRRKGVEVKMYSF*ERKGLVYQEIS*PQDDDYL 1
2 YCEKCQNFFIDTCESHGVPTFVKNSTTDSGHPNHLALTPSSGLRTRPSSIPKAWLRLWNKAFELLLGLPFSPCEGQVIEDEAVDNSGYSWL 0
0 ITKGRNCYEYVDGKDNSWANWMR 2
1 YVNGTQDEKEQNLVFFQYHRQIFYQTCYAVWPGCQLLVWYRDECGQELGIKWDNRGKKEFe 1
2 EPKPEAHPCPSCPLAFSSEKFLSQHMKHNHPSQSSPETPERKHLQPEDPHPGHQNQQQQQHSDPHRWNDKAEGQQTGDRSKPMFENIRQEVTSRAFSSLPKGQMVCSREGNRMMETEPSPGLKVNPEVTGKLFLGVESSRIAK
VKYRGCGRDFSDRSHQSGHQRRHQKKP
SVCKKVKREFSHKSVLITHQRTHSGEKS
YVCKESGRGFSAKSNLIRPRRTHTGEKP
YVCGERG*GFSV*SGLIIHQRAHSPEKP
YVCREGRRGFGDKSSFIKHQRATLGEKS
YVCKESGRGFSAKSNLIRPRRKKCRHDTTPHPQL* 0

>PRDMx_danRer Danio rerio (zebrafish) Q6P2A1 transcript BC064665 pseudo-homologous
0 MSLSPDLPPSEEQNLEIQGSATNCYSVVIIEEQDDTFNDQPF 1
2 YCEMCQQHFIDQCETHGPPSFTCDSPAALGTPQRALLTLPQGLVIGRSSISHAGLGVFNQGQTVPLGMHFGPFDGEEISEEKALDSANSWV 0
0 ICRGNNQYSYIDAEKDTHSNWMK 2
1 FVVCSRSETEQNLVAFQQNGRILFRCCRPISPGQEFRVWYAEEYAQGLGAIWDKIWDNKCISQ 1
2 GSTEEQATQNCPCPFCHYSFPTLVYLHAHVKRTHPNEYAQFTQTHPLESEAHTPITEVEQCLVASDEALSTQTQPVTESPQEQISTQNGQPIHQTENSDEPDASDIYTAAGEISDEI
HACVDCGRSFLRSCHLKRHQRTIHSKEKP
YCCSQCKKCFSQATGLKRHQHTH QEQEKNIESPDRPSDI
YPCTKCTLSFVAKINLHQHLKRH HHGEYLRLVESGSLTAETEEDHT
EVCFDKQDPNYEPPSRGRKSTKNSLKG
RGCPKKVAVGRPRGRPPKNKNLEVEVQKIS
PICTNCEQSFSDLETLKTHQCPRRDDEGDNVEHPQEASQ
YICGECIRAFSNLDLLKAHECIQQGEGS
YCCPHCDLYFNRMCNLRRHERTIHSKEKP
YCCTVCLKSFTQSSGLKRHQQSHLRRKSHRQSSALFTAAIFPCAYCPFSFTDERYLYKHIRRHHPEMSLKYLSFQEGGVLSVEKP
HSCSQCCKSFSTIKGFKNHSCFKQGEKV
YLCPDCGKAFSWFNSLKQHQRIHTGEKP
YTCSQCGKSFVHSGQLNVHLRTHTGEKP
FLCSQCGESFRQSGDLRRHEQKHSGV
RPCQCPDCGKSFSRPQSLKAHQQLHVGTKL
FPCTQCGKSFTRRYHLTRHHQKMHS* 0

>ZNF133_homSap Homo sapiens (human) NP_001076799 KRAB Krueppel-associated box and zinc fingers
0 MAFRDVAVDFTQDEWRLLSPAQRTLYREVMLENYSNLVSL 1
2 GISFSKPELITQLEQGKETWREEKKCSPATCP 1
2 DPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPGPGDQ EKQQQASEGRPWSDQAEGPE GEGAMPLFGRTKKRTLG AFSRPPQRQPVSSRNGLRGVELEASPAQTGNPEETDKLLKRIEVLGFGT
VNCGECGLSFSKMTNLLSHQRIHSGEKP
YVCGVCEKGFSLKKSLARHQKAHSGEKP
IVCRECGRGFNRKSTLIIHERTHSGEKP
YMCSECGRGFSQKSNLIIHQRTHSGEKP
YVCRECGKGFSQKSAVVRHQRTHLEEKT
IVCSDCGLGFSDRSNLISHQRTHSGEKP
YACKECGRCFRQRTTLVNHQRTHSKEKP
YVCGVCGHSFSQNSTLISHRRTHTGEKP
YVCGVCGRGFSLKSHLNRHQNIHSGEKP
IVCKDCGRGFSQQSNLIRHQRTHSGEKP
MVCGECGRGFSQKSNLVAHQRTHSGERP
YVCRECGRGFSHQAGLIRHKRKHSREKP
YMCRQCGLGFGNKSALITHKRAHSEEKP
CVCRECGQGFLQKSHLTLHQMTHTGEKP
YVCKTCGRGFSLKSHLSRHRKTTSVHHR LPVQPDPEPCAGQPSDSLYSL* 0

>ZNF343_homSap Homo sapiens (human) KRAB Krueppel-associated box and zinc fingers
0 MMLPYPSALGDQYWEEILLPKNGENVETMKKLTQNHKAK 1
2 GLPSNDTDCPQKKEGKAQIV 0
0 VPVTFRDVTVIFTEAEWKRLSPEQRNLYKEVMLENYRNLLSL 1
2 AEPKPEIYTCSSCLLAFSCQQFLSQHVLQIFLGLCAENHFHPGNSSPGHWKQQGQQYSHVSCWFENAEGQERGGGSKPWSARTEERETSRAFPSPLQRQSASPRKGNMVVETEPSSAQRPNPVQLDKGLKELETLRFGA
INCREYEPDHNLESNFITNPRTLLGKKP
YICSDCGRSFKDRSTLIRHHRIHSMEKP
YVCSECGRGFSQKSNLSRHQRTHSEEKP
YLCRECGQSFRSKSILNRHQWTHSEEKP
YVCSECGRGFSEKSSFIRHQRTHSGEKP
YVCLECGRSFCDKSTLRKHQRIHSGEKP
YVCRECGRGFSQNSDLIKHQRTHLDEKP
YVCRECGRGFCDKSTLIIHERTHSGEKP
YVCGECGRGFSRKSLLLVHQRTHSGEKH
YVCRECRRGFSQKSNLIRHQRTHSNEKP
YICRECGRGFCDKSTLIVHERTHSGEKP
YVCSECGRGFSRKSLLLVHQRTHSGEKH
YVCRECGRGFSHKSNLIRHQRTH* 0

>ZNF169_homSap Homo sapiens (human) KRAB Krueppel-associated box and zinc fingers
0 MSPGLLTTRKEALMAFRDVAVAFTQKEWKLLSSAQRTLYREVMLENYSHLVSL 1
2 GIAFSKPKLIEQLEQGDEPWREENEHLLDLCP 1
2 EPRTEFQPSFPHLVAFSSSQLLRQYALSGHPTQIFPSSSAGGDFQLEAPRCSSEKGESGETEGPDSSLRKRPSRISRTFFSPHQGDPVEWVEGNREGGTDLRLAQRMSLGGSDTMLKGADTSESGAVIRGNYRLGLSKKSSLFSHQKH
HVCPECGRGFCQRSDLIKHQRTHTGEKP
YLCPECGRRFSQKASLSIHQRKHSGEKP
YVCRECGRHFRYTSSLTNHKRIHSGERP
FVCQECGRGFRQKIALLLHQRTHLEEKP
FVCPECGRGFCQKASLLQHQSSHTGERP
FLCLECGRSFRQQSLLLSHQVTHSGEKP
YVCAECGHSFRQKVTLIRHQRTHTGEKP
YLCPQCGRGFSQKVTLIGHQRTHTGEKP
YLCPDCGRGFGQKVTLIRHQRTHTGEKP
YLCPKCGRAFGFKSLLTRHQRTHSEEEL
YVDRVCGQGLGQKSHLISDQRTHSGEKP
CICDECGRGFGFKSALIRHQRTHSGEKP
YVCRECGRGFSQKSHLHRHRRTKSGHQL LPQEVF* 0

>ZNF596_homSap Homo sapiens (human) KRAB Krueppel-associated box and zinc fingers
0 MESQESVTFQDVAVDFTQEEWALLDTSQRTLFREVMLENISHLVSV 1
2 GNQLYKSDVISHLEQGEQLSREGLGFLQGQSPVISDREDDPKKQEMLSMQHICKKDAPLISAMQWSHTQEDPLECNNFREKFTEILPLTQYVIPQVGKKPFISQDVGKAISYLPSFNIQKQIHSRSKS
YECHQRRNTFIQSSAHRQHNNTQTGEKT
FECHVCRKAFSKSSNLRRHEMIHTGVKP
HGCHLCGKSFTHCSDLRKHERIHTGEKL
YGCHLCGKAFSKSYNLRRHEVIHTKEKP
NECHLCGKAFAHCSDLRKHERTHFGEKP
YGCHLCGKTFSKTSYLRQHERTHNGEKP
YGCHLCGKAFTHCSHLRKHERTHTGEKP
YECHLCGKAFTESSVLRRHERTHTGEKP
YECHLCWKAFTDSSVLKRHERTHTGEKP
YECHLCGKTFNHSSVLRRHERTHTGEKP
YECNICGKAFNRSYNFRLHKRIHTGEKP
YKCYLCGKAFSKYFNLRQHENSCYKGNK* 0

>HKR1_homSap Homo sapiens (human) KRAB Krueppel-associated box and zinc fingers
0 MRVNHTVSTMLPTCMVHRQTMSCSGAGGITAFVAFRDVAVYFTQEEWRLLSPAQRTLHREVMLETYNHLVSL 1
2 EIPSSKPKLIAQLERGEAPWREERKCPLDLCP 1
2 ESKPEIQLSPSCPLIFSSQQALSQHVWLSHLSQLFSSLWAGNPLHLGKHYPEDQ KQQQDPFCFSGKAEWIQE GEDSRLLFGRVSKNGTSKALSSPPEEQQPAQSKEDNTVVDIGSSPERRADLEETDKVLHGLEVSGFGE
IKYEEFGPGFIKESNLLSLQKTQTGETP
YMYTEWGDSFGSMSVLIKNPRTHSGGKP
YVCRECGRGFTWKSNLITHQRTHSGEKP
YVCKDCGRGFTWKSNLFTHQRTHSGLKP
YVCKECGQSFSLKSNLITHQRAHTGEKP
YVCRECGRGFRQHSHLVRHKRTHSGEKP
YICRECEQGFSQKSHLIRHLRTHTGEKP
YVCTECGRHFSWKSNLKTHQRTHSGVKP
YVCLECGQCFSLKSNLNKHQRSHTGEKP
FVCTECGRGFTRKSTLSTHQRTHSGEKP
FVCAECGRGFNDKSTLISHQRTHSGEKP
FMCRECGRRFRQKPNLFRHKRAHSGA
FVCRECGQGFCAKLTLIKHQRAHAGGKP
HVCRECGQGFSRQSHLIRHQRTHSGEKP
YICRKCGRGFSRKSNLIRHQRTHSG* 0

>PRDM11_homSap Homo sapiens (human) 511 aa 7 exons chr11:45115564 44% id PRDM9 SET
0 MLKMAEPIASLMIVECRACLRCSPLFLYQREK 0
0 DRMTENMKECLAQTNAAVGDMVTVVKTEVCSPLRDQEYGQPC 2
1 SRRPDSSAMEVEPKKLKGKRDLIVPKSFQQVDFW 1
2 FCESCQEYFVDECPNHGPPVFVSDTPVPVGIPDRAALTIPQGMEVVKDTSGESDVRCVNEVIPKGHIFGPYEGQISTQDKSAGFFSWL 0
0 IVDKNNRYKSIDGSDETKANWMR 2
1 YVVISREEREQNLLAFQHSERIYFRACRDIRPGEWLRVWYSEDYMKRLHSMSQETIHRNLAR 1
2 GEKRLQREKSEQVLDNPEDLRGPIHLSVLRQGKSPYKRGFDEGDVHPQAKKKKIDLIFKDVLEASLESAKVEAHQLALSTSLVIRKVPKYQDDAYSQCATTMTHGVQNIGQTQG
EGDWKVPQGVSKEPGQLEDEEEEPSSFKADSPAEASLASDPHELPTTSFCPNCIRLKKKVRELQAELDMLKSGKLPEPPVLPPQVLELPEFSDPAGKLVWMRLLSEGRVRSGLCGG* 0

>PRDM1_homSap Homo sapiens (human) 825 aa 7 exons chr6 106,546,004 3DAL:KMDM..NLTQ SET + 5 C2H2
0 MLDICLEKRVGTTL 0
0 AAPKCNSSTVRFQGLAEGTKGTMKMDMEDADMTLWTEAEFEEKCTYIVNDHPWDSGADGGTSVQAEASLPRNLLFKYATNSEE 0
0 VIGVMSKEYIPKGTRFGPLIGEIYTNDTVPKNANRKYFWR 0
0 IYSRGELHHFIDGFNEEKSNWMRYVNPAHSPREQNLAACQNGMNIYFYTIKPIPANQELLVWYCRDFAERLHYPYPGELTMMNL 1
2 TQTQSSLKQPSTEKNELCPKNVPKREYSVKEILKLDSNPSKGKDLYRSNISPLTSEKDLDDFRRRGSPEMPFYPRVVYPIRAPLPEDFLKASLAYGIERPTYITRSPIPSSTTPSPSARSS
PDQSLKSSSPHSSPGNTVSPVGPGSQEHRDSYAYLNASYGTEGLGSYPGYAPLPHLPPAFIPSYNAHYPKFLLPPYGMNCNGLSAVSSMNGINNFGLFPRLCPVYSNLLGGGSLPHPMLNPTS
LPSSLPSDGARRLLQPEHPREVLVPAPHSAFSFTGAAASMKDKACSPTSGSPTAGTAATAEHVVQPKATSAAMAAPSSDEAMNLIKNKRNMTGYKTLPYPLKKQNGKIKYECNVCAKTFGQLSNLK 0
0 VHLRVHSGERPFKCQTCNKGFTQLAHLQKHYLVHTGEKPHECQ 0
0 VCHKRFSSTSNLKTHLRLHSGEKPYQCKVCPAKFTQFVHLKLHKRLHTRERPHKCSQCHKNYIHLCSLKVHLKGNCAAAPAPGLPLEDLTRINEEIEKFDISD
NADRLEDVEDDISVISVVEKEILAVVRKEKEETGLKVSLQRNMGNGLLSSGCSLYESSDLPLMKLPPSNPLPLVPVKVKQETVEPMDP*

>PRDM4_homSap Homo sapiens (human) 801 aa 11 exons chr12:108126644 3DB5:EHGPV..IGVPE SET + 1 + 6 C2H2 domaians
0 MHHR 2
1 MNEMNLSPVGMEQLTSSSVSNALPVSGSHLGLAASPTHSAIPAP 1
2 GLPVAIPNLGPSLSSLPSALSLMLPMGIGDRGVMCGLPERNYTLPPPPYPHLESSYFRTILP 1
2 GILSYLADRPPPQYIHPNSINVDGNTALSITNNPSALDPYQSNGNVGLEPGIVSIDSRSVNTHGAQSLHPSDGHEVALDTAITMENVSRVTSPISTDGMAEELTMDGVAGEHSQIPNGSRSHEPLSVDSVSN
NLAADAVGHGGVIPMHGNGLELPVVMETDHIASRVNGMSDSALSDSIHTVAMSTNSVSVALSTSHNLASLESVSLHEVGLSLEPVAVSSITQEVAMGTGHVDVSSDSLSFVSPSLQMEDSNSNKENMATLFTI 1
2 WCTLCDRAYPSDCPEHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGAEV 1
2 GVWTGETIPVRTCFGPLIGQQSHSMEVAEWTDKAVNHIWK 0
0 IYHNGVLEFCIITTDENECNWMMFVRKAR 2
1 NREEQNLVAYPHDGKIFFCTSQDIPPENELLFYYSRDYAQQI 1
2 GVPEHPDVHLCNCGKECNSYTEFKAHLTSHIHNHLPTQGHSGSHGPSHSKERKWKCSMCPQAFISPSKLHVHFMGHMGMKPHKCDFCSKAFSDPSNLRTHLKIHT 1
2 GQKNYRCTLCDKSFTQKAHLESHMVIHTGEKNLKCDYCDKLFMRRQDLKQHVLIHTQ 2
1 ERQIKCPKCDKLFLRTNHLKKHLNSHEGKRDYVCEKCTKAYLTKYHLTRHLKTCKGPTSSSSAPEEEEEDDSEEEDLADSVGTEDCRINSAVYSADESLSAHK* 0

>GAS8_homSap Homo sapiens (human) synteny marker right centromeric positive strand C16orf3- in second intron growth arrest-specific del cancer
MAPKKKGKKGKAKGTPIVDGLAPEDMSKEQVEEHVSRIREELDREREERNYFQLERDKIHTFWEITRRQLEEKKAELRNKDREMEEAEERHQVEIKVYKQKVKHLLYEHQNNLTEMKAEG
TVVMKLAQKEHRIQESVLRKDMRALKVELKEQELASEVVVKNLRLKHTEEITRMRNDFERQVREIEAKYDKKMKMLRDELDLRRKTELHEVEERKNGQIHTLMQRHEEAFTDIKNYYNDI
TLNNLALINSLKEQMEDMRKKEDHLEREMAEVSGQNKRLADPLQKAREEMSEMQKQLANYERDKQILLCTKARLKVREKELKDLQWEHEVLEQRFTKVQQERDELYRKFTAAIQEVQQKT
GFKNLVLERKLQALSAAVEKKEVQFNEVLAASNLDPAALTLVSRKLEDVLESKNSTIKDLQYELAQVCKAHNDLLRTYEAKLLAFGIPLDNVGFKPLETAVIGQTLGQGPAGLVGTPT*