Opsin evolution: Neuropsin phyloSNPs: Difference between revisions

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A third paralogous family NEUR3 was [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed_pubmed&uid=18570255 reported in July 2008] and characterized by syntenic relations and expression in chicken. However in chicken, NEUR1 actually is most abundant of the three paralogs in developing and early posthatch neural retina, notably in differentiating ganglion cells and amacrine cells.  
A third paralogous family NEUR3 was [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed_pubmed&uid=18570255 reported in July 2008] and characterized by syntenic relations and expression in chicken. However in chicken, NEUR1 actually is most abundant of the three paralogs in developing and early posthatch neural retina, notably in differentiating ganglion cells and amacrine cells.  


Recoverable sequences range from lamprey to shark to fish (where a further lineage-specific tandem duplication has occured) to frog to sauropsids, with the gene again lost in all mammals including platypus. This locus exhibits an ancestral fusion of exon 2-3 relative to neuropsin and newropsin. The tandem duplication in rayfinned fish could easily be mistaken for a whole genome duplication effect -- however there is no sign of this for any of the 3 paralogs in any of the 5 fish with assembled genomes.
Recoverable sequences range from lamprey to shark to fish (where a further lineage-specific tandem duplication has occured) to frog to sauropsids, with the gene again lost in all mammals including platypus. This locus exhibits an ancestral fusion of exon 2-3 relative to neuropsin and newropsin. The tandem duplication in rayfinned fish could easily be mistaken for a whole genome duplication effect -- however there is no sign of such an event for any of the 3 paralogs in any of the 5 fish with assembled genomes.
 
The NEUR3 group could possibly signal through heteromeric G protein. The DRY motif is a bit unusual, consisting of V/I R F/Y whereas the YNPxIY x aliphatic is fairly conventional. The Schiff base K is preserved in both NEUR3a and NEUR3b sequences.


The other oddity is NEUR1 and NEUR3 are on the same chromosome for all species examinable. They are separated by a million or so bp as well as other coding genes. This possibly represents an old tandem duplication that experienced subsequent rearrangements. On the whole, synteny has been quite well preserved for all NEUR genes:
The other oddity is NEUR1 and NEUR3 are on the same chromosome for all species examinable. They are separated by a million or so bp as well as other coding genes. This possibly represents an old tandem duplication that experienced subsequent rearrangements. On the whole, synteny has been quite well preserved for all NEUR genes:
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  NEUR2_anoCar  -DSC1  +DSG2  +TTR  -B4GALT6  -NEUR2  -K1012  +RNF138  .....  .....
  NEUR2_anoCar  -DSC1  +DSG2  +TTR  -B4GALT6  -NEUR2  -K1012  +RNF138  .....  .....


[[Image:NeurAll.jpg]]


A proposed revised terminology for this family follows. Note NEUR2 and NEUR3 will never receive official HGCN nomenclature because (like thousands of amniote ancestral genes) they are absent in human and mouse. Here lower case a and b are used in the case of lineage-specific duplications, with a reserved for the copy with higher blastp score to human (or, if absent, nearest species).
A proposed revised terminology for this family follows. Note NEUR2 and NEUR3 will never receive official HGCN nomenclature because (like thousands of amniote ancestral genes) they are absent in human and mouse. Here lower case a and b are used in the case of lineage-specific duplications, with a reserved for the copy with higher blastp score to human (or, if absent, nearest species).


  Gene  Protein    HGCN  Synonyms  Lineage-specific duplicate  DRY NPxxY K Accessions  
  Gene  Protein    HGCN  Synonyms  Lineage-specific duplicate  DRY YNPxxY K Accessions  
              
              
  NEUR1  neuropsin  OPN5  cOpn5m    NEUR1a/b cephalochordate    DRY NPIIY K NM_181744 NM_001130743
  NEUR1  neuropsin  OPN5  cOpn5m    NEUR1a/b cephalochordate    DRY YNPIIY K NM_181744 NM_001130743
  NEUR2  newropsin  ----  cOpn5L1                              VCC NPxxY K          XM_419178
  NEUR2  newropsin  ----  cOpn5L1                              VCC YNPxIY K          XM_419178
  NEUR3  newtopsin  ----  cOpn5L2  NEUR3a/b actinopterygii    DRY NPxxY K          XM_420056
  NEUR3  newtopsin  ----  cOpn5L2  NEUR3a/b actinopterygii    vRf YNPxIY K          XM_420056
 
[[Image:NeurAll.jpg]]


== Curated Set of 52 deuterostome neuropsins ==
== Curated Set of 52 deuterostome neuropsins ==

Revision as of 14:11, 19 January 2009

Neuropsin backgrounder

Neuropsin (OPN5) is a deeply diverged member of the opsin family with a single publication on it and considerable confusion over the name (mostly used for an unrelated kalikrein serine protease KLK8, not an opsin). There are no known disease associations or described knockout phenotypes; it is expressed primarily in brain, spinal chord, and testes.

Neuropsin has all the classical attributes of a rhodopsin-class GPCR and indeed opsin photoreceptor: Schiff base lysine at expected position, standard tyrosine counterion and DRY motif, seven transmembrane configuration, disulfide at expected position, proximal glycosylation and distal palmitoylation and kinase sites. It is most closely related to peropsin and rgropsin in terms of blast clustering and intron positioning. Its G-protein signalling partner is not known.

Its evolution is illuminated by the massive comparative genomics study described here, which extractes and compares over 50 full length sequences from various genomics projects. Neuropsin can be located outside chordates but not outside deuterostomes. However, like peropsin and rgropsin, it must have originated much earlier in pre-Bilaterans. Thus its absence in earlier diverging species must be due either to gene loss or unrecognizability. Some role in deuterostomes however has persisted in deuterostomes over billions of years of branch length.

Within placental mammals, neuropsin is extraordinarily conserved, with percent identity relative to human protein 96% averaged over 31 species (likely above the 95% percentile of all coding genes proteomewide). That conservation drops considerably at marsupials and monotremes (86%), is less striking at tetrapods (78%), and not especially remarkable at teleost fish (68%). This pattern suggests neuropsin acquired significant new adaptive functionality on the placental mammal stem, leading to marked resilience to fixation of any further variation.

Opsin NeurStop.png

The structure of the neuropsin gene is rather odd at the 3' end. In human, a weak splice signal appears to have developed that results, after an intron of 12,244 bp, in a seventh very short exon followed by a long 3'UTR. However a stop codon is soon encountered if the splice is not taken (which it is in 6 of 7 transcripts). This results in two slightly different alternative carboxy termini sequences QEV* vs QEWE*. Very few transcripts exist in this region for any species but it appears that the recent ancestral form of the protein only utilized the inital stop codon in exon 6.

This feature GTatga is conserved in all species back to platypus; indeed an unexplained conservation in nucleotide sequence extends well beyond this. However the splice acceptor and WE* appear an option only back to rhesus, whereas the QEV* option is available in all 38 available mammal genomic sequences. The Q itself is a strong phyloSNP characteristic of mammals from platypus through lemurs.

A single mouse transcript also terminates early; four others continue on. No species other than rat has an available transcript in this region. No other rodent genomes could have an orthologous exon: kangaroo rat and ground squirrel have frame shifts, pika lacks the splice acceptor, and rabbit and guinea pig have no homologous sequence.

This can be explained by independent origins of splice acceptors in various clades. However far more comparative transcripts are needed to understand the 3' end of this gene. It may be that the conserved intronic sequence following exon 6 is an accident waiting to happen as it conserves -- for whatever reason -- half of a splice site.


Novel neuropsins in amphioxus and sea urchin

The genome of Branchiostoma (amphioxus, lancelet) contains two distinct neuropsins about 75% identical to each other and 42% to human. These cluster unambiguously with vertebrate opsins and share critical conserved residues. An extra intron distinguishes them from the vertebrate neuropsin pattern. Recall Branchiostoma species has three rather diverged (and well-studied) peropsins but no evident Rgr opsin. These raises the question whether neuropsin and peropsin developed substantial visual roles in this species as an alternative to the ciliary imaging opsin pathway seen by lamprey divergence. Sea urchins, but not acornworm Saccoglossus, contain a single neuropsin that is quite diverged.

These neuropsins are newly reported here, meaning they were not localized in recent in situ hybridization studies. That's especially unfortunate in view of the antecedent role the Branchiostoma ancestral node plays in the evolution of chordate eye and the complexities of photoreceptor tissues in the extant species.

PhyloSNPs in vertebrate neuropsins

Alignment analysis coming shortly. Neuropsin has rather few of them. 

position  ...................................................................................................1.........1.........1.........1.........1.........1.........1.........1......1..
position  .........1.........2.........3.........4.........5.........6.........7.........8.........9.........0.........1.........2.........3.........4.........5.........6.........7......7..
position  123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567..
excMemCy  eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMcccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeee..
keyResid  ...GLC.................................................................................................diS..cIon.................DRY...............................................
exonNumb  111111111111111111111111111111111111111111122222222222222222222222222222222222222223333333333333333333333333333333333333333333333333333333334444444444444444444444444444444444444..
10homSap  MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPE..
11panTro  ...................................................................................................................................................................................
12gorGor  ............................................................................................................................................------------------.....................
13ponPyg  ...................................................................................................................................................................................
14nomLeu  ...................................................................................................................................................................................
15rheMac  .................................I............................................................................................................................................A....
16papHam  .................................I............................................................................................................................................A....
17calJac  ......S.......................................................................................................................................................................A....
18tarSyr  ....................................................................................I.........................................................................................A....
19otoGar  ............LR..........................................................V...................................................................----------------------..M......E.......
20micMur  ......V.................................I..........................................---------------------------------------------------------..............L....V..............A....
21tupBel  ............S.............................................................................................................................................V...................A....
22musMus  ....................E..................................................................................F..................................................V...................A....
23ratNor  ....................E..................................................................................F..................................................V...................A....
24speTri  ............H.......E................................................................................................................................F....V...................A....
25dipOrd  ..F....GT.GQG.....PEE...T........I........................................................................................................................V........................
26cavPor  .......P..N.H..R..Q.E...V.......................V...........................I..................R.............................V.................................V..............A....
27oryCun  ............H......E..............................................................................................................................R.......L...................A....
28ochPri  ....D.......H....F.........................................................................................L....D.....................------......R.......V...................A....
29canFam  .......R...........E........................................................I.............................................................................V...................A....
30felCat  .......P...........E.................................................--------------.......................................................................V...................A....
31bosTau  .......P.P...R.P........................................................V...I................................................................I............V.....A.............A....
32turTru  ......................K..........I.............................................................................V...................A....
33susScr  .......P.P...R.....E....................................................V...I.............................................................................V.....A.............A....
34vicVic  .......P.P...R.RH...............................L..................................................M........................................-------------------------------------..
35equCab  ............................................................................I.............................................................................V........................
36myoLuc  ............G.....Q.............................V.......................V...........................................................................T.....F........................
37pteVam  ......V.....H....V..............................V...........................I.............................................................................V........................
38sorAra  ............N..........................................................................................M.................................................VV...................A....
39eriEur  .S..Q.......G.........................................................................................................................................L...V...................A....
40loxAfr  .T.....P...D...Q..Q.....T.................................C..............................V................................................................V...................A....
41proCap  .T....V..E.D..S.........T................V......C.........Y..............................I...S..........................................H...-------------------------------------..
42echTel  .......P...NS...........V.........G.....I.................Y....................................S.......T..................................................V........................
43dasNov  ...........D...............................................K..............................................................................................V........................
44choHof  ......G....DS....F......................I....................R............................................................................................V.............L..........
45monDom  .....SVS...DYI..............................V...L.....I....K............V......................S.......V................................H....T....H..F....L.....T..A.V.FA.V.S.A....
46macEug  .V...L.....I....K............V..............................V................................H....T....H......VI.....T..A....A...N.A....
47ornAna  MT.YS.PQLGDY......E....V............V..V...V...L.....I.................V..................V...........M................................H....T....H.......I........A........N.A....
48galGal  ..SDCNSSS.E.Y....MQQE........R...II......V......L.....IF...K............V................S...F.S...I...M................................H.A..T....H..F....L.....T..A.V.FA.V.S.A....
49taeGut  ..SEYNNSS.E.YI....QEE........R...II.............L.....IF...K............V................S...F.S...M......C.............................H....T....H..F....I.....M..A.V.FA.V.S.A....
50anoCar  .EQGQNISS..DN----QQEE........V...I...V..LV......L.....I...TQ.....K......V................S..AF.S...I...S.............I..............F...H....T....H.VF...GI..S..A..A.I.FA.F.N.A....
51xenTro  ..G.SSYREESGYI...E..S........R...IF..V..MA......L.....I..ACS........................T....A.V...S.......NA..................L.V..........H.R..T....R..F.A..V.....TL.A.L....V.N.A....
52danRer  .E-NET-SISSGYI....LR.........K...I..A..ILV.....AT.....M..TFK..T..K.P....L...IF.F....S....F.V.S.S...L...Q...Y.................I..F.......H.R..T....H..FLSVVF.....A..A...V..W.N.A....
53pimPro  .E-NDT-SIPSGYV....LR.........K...I..A..ILV..V..AT.....I.QTIK..T..K.P.F..L....F.F....T....F.V.S.S...L...Q...Y.................I..........H.R..T.F..H..FL..VFT.L..A..A...V..W.N.A....
54takRub  .E-NET-WTHSSYV....LR......R..K...I..AL.IC...LM.AT........TFK..T..K.P.L..L...IF.F....T....F.V.SLS...L...E...F.................V..........H.R..A....H..FL...SV....A..A......W.S.A....
54tetNig  .D-NET-RSHPSYV....LR......R..K...I..A..IF...VM.AT........TFK..T..K.P.L..V...IF.F....T....F.V.SLS...L...E...F.................V..........H.R..T....H..FV...LV....A..A......W.S.A....
56gasAcu  .E-NET-WTHPSYI....LR......R..K...II.A..IC....M.AT.....I..TIK..S..K.P.L..V....F.F....T....FVV.S.A...L...E...F.................V..........H.R..T....Q..FL..VFV.M..A..A......W.N.A....
57oryLat  .E-N.S-W.HSSYV....LR......R..K...I..A..IL....M.AT.....I..TIK..S..K.P.L..V....F.F....T....FVV.S.S...L...E...F.................V..........H.R..T....Q.IFL..VFV.I..A..A......W.S.A....
59calMil  .TFDNSTALYSGYWL.DSLH....V........IISAC..IVT.L...L.....I.L.ITQ.R..K.P..LIT...IS.F.M..G.Q..L.....S...I...V....H................V..........H.Q..S..Q.R.VFMS..F..F..A..A......W.N.A....
10homSap  MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPE..
phyloSNP  ..................AA......B......A..A..B.....B.BB.........B......C.A.........B.A....A..........B......................................A...B..A.......A.............A...............
  ..
  ..
position  1.1.........1.........2.........2.........2.........2.........2.........2.........2.........2.........2.........2.........3........3..........3.........3.........3.........3.....3
position  7.8.........9.........0.........1.........2.........3.........4.........5.........6.........7.........8.........9.........0........1..........2.........3.........4.........5.....6
position  89012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456
excMemCy  eeeeeeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMccccccccccccccccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMcccccccccccccccccccccccccccccccccccccccccccccc*
keyResid  ...diS................................................................................................................K...........................................
exonNumb  44444444444444444444444444444444444444444444444444444444444444444444444444455555555555555555555555555555555555555555555555555555555555555555555555555555555556666666666666666666666
10homSap  PFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKS-LEGFRLHTVT-TVRKSSAVLEIHEEV*
11panTro  .................................................................................................................................................E.....-..........-...............*
12gorGor  ...............................................................P.....................................K.................................................-....Q.....-...............*
13ponPyg  .....................................................................................................S.................................................-..........-...............*
14nomLeu  .......................................................................................................................................................-..........-S..............*
15rheMac  .......................................................................................................................................................-..........-...............*
16papHam  .......................................................................................................................................................-..........-...............*
17calJac  .......................................................................................................................................................-..D.......-...............*
18tarSyr  ......................................................G.........T......................................................................................-..D.......-...............*
19otoGar  ..-...........-.L...............................................G................................................................................T.....-..D.......-............Q..*
20micMur  .......................................................I.............................................................................----------------------H......-A...........Q..*
21tupBel  ....................I..................................I..........................................................................................................-............Q..*
22musMus  ................G.......S.................A...........................V...............................................................R.....A...RG.....-..D.......-............Q..*
23ratNor  ................G.......S.................A...........................V................................N..............................R.........R......-..D.......-A...........Q..*
24speTri  .......................-..............E..............E..............-------.......................................S....................................-..D.......-A.......V...Q..*
25dipOrd  ................LA.................S...........................P.......................................................................................-.......................Q..*
26cavPor  ................A...I...H...........M..................I.............................................................................SR.....NA.........-..D.......-.D...-......Q..*
27oryCun  ........................................................................................................................................S..R.S.........-..D.......-............Q..*
28ochPri  ................................................................G.......................................................................S..R.....Q.....-..D.......-............Q..*
29canFam  ................L...I.........................................................................................................................R........-..D...N...-............Q..*
30felCat  ....................I..................................................................................................................................-..D.......-............Q..*
31bosTau  ....................I.................................................V................................................................................-..D.......-..........V.Q..*
32turTru  ....................I..........................................P..........................................................V............................-..D.......-............Q..*
33susScr  .....................................................................................V.................................................................-..D.......-...........RQ..*
34vicVic  ---------------------------------------------------------------------------............................................................................-..D....A..-............Q..*
35equCab  ....................I...........................................G.....V................................................................................-..D.......-............Q..*
36myoLuc  ...............T....I.................................K.........-----------.............................................S..............L........R......-..N.......-............Q..*
37pteVam  ....................I...........................................G..M.........................................................................S..R......-..D.....I.-...EA.......Q..*
38sorAra  ....................I..................................................................................N..............................R.....S...R......-.DD.......-...E........Q..*
39eriEur  ................L...I.................................K............M..............................................................................N....-.KDY...................q..*
40loxAfr  ....................I..................................I...........M............................................................................R......-..........-..K.......V.Q..*
41proCap  ---------------------------------------------------------------------------..............................V.................................R.R..R...E..-...V......-............Q..*
42echTel  .......................................................I...........M............................................................................................I.-...........HQ..*
43dasNov  ................................................................................................................................................R......-..D.......-...E......V.Q..*
44choHof  ...................................................................M.......................................................................R....R......-F.........-............Q..*
45monDom  .................A..A.V.S.......F.............L.....T.....Y.T..QN..I.................................Q.....V.F.......................C......S..Q..A..E.-.RTY......-...R........Q..*
46macEug  .................T..T...............................T..........Q..............................................................................................RHTVSTIRKSSSVSETYQ..*
47ornAna  .................A..A...............................T..........QN....................................Q.......F.......................CRIS..RL..P.TG..E.-.KNS.S.SMS-.I..P...SGP.Q..*
48galGal  .................A..A.V.S.......F.............L.....T.....Y.T..QN..I.................................Q...V...F.......................C.....RS..P.TLQ...S.KES.MY.IS-SH.D.A.LSGTQL..*
49taeGut  .................A....V.S.....................L.....T.....Y.T..QN..I.................L...................V...F......................ECRL...RP..*
50anoCar  ..............G..A..A..........V......M.C........Q..T.....Y.T..QNQ..................MF...................V..KV..I.............V......C.S...RP.N.QPLQ..NSR*
51xenTro  ....T............K..I.V.SM......F..M......A.........A.......T.NQNN.T..I.................F............Q......E......MM....S...........C.P...KKD--.SLQNTT----S.VY.IS-.F...TTSAR*
52danRer  ............T....S..S.VMCM.....IF..VI......M..F.....A...S...T.NKNN.S............................M....E...V..PV..........S............C.KK.VKSCCFQ.WR..KPSKTS.FY.ISGSIKQRPGD-.ASI.I*
53pimPro  .................S..S.VMCM.I...V...G.......M..LQ....AQ..S...TQNKNK.H............................V....D...V..SI..........S............C.KN.AKLSCFQ.WS.RKHYKTS.FYSISASMK.RP.N-.VPT.I*
54takRub  .................S..S.VMA......I...GI......M..F.....A..IS...A..RN..D..I.........................I....E...V..PV..I.......S..........V.V.TS.TNFSCC..L.ERIHFRKS..Y.ISGSL.DPLPPK.A.I.M*
54tetNig  ....A............S..S.VMA......V...GI.....IM..F.....A..IS...A..KN..S..I.........................V....E...V..PV..I.......S..........A.L.TS.TSSSCC..L.ERVLFRKA..Y.ISGSL.DTLPPK.A.I.M*
56gasAcu  .................S..S.VVA......V..AGI......M..F.....A..ISN..A..KN..N..I.........................V....E...V..SV..I.......S..........L.L.NS.MKSSCF.GL..PRHFRKS.FY.ISGS.KDNTTAK.AQI.M*
57oryLat  .................S..S.VMS......V..AGI......M..C.........SS..A..KN..T..I.........................V....E......PV..I.......S.........LV.L.NS.-S.CCA.VIR.RTHFRNS.FY.ISGSLKDTAPAK.A.I.I*
59calMil  .............RV..S.LI.V.T.........III.....I.........A..........QNH.S...N..........................................................................................................*
10homSap  PFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKS-LEGFRLHTVT-TVRKSSAVLEIHEEV*
phyloSNP  ...........................................B............B......CA.......................................................B.................................B...B.BBA............A..*

Neuropsin (NEUR1 compared to newropsin (NEUR2)

Newropsins are a new opsin gene family -- first reported here -- most closely related to neuropsins (42% percent identity) and next to melanopsins and peropsins. Like so many opsin families, they persist from chondrichthyes to archeosaurs but vanish without a trace in platypus, marsupials, and placentals. (The syntenic order B4GALT6 NEUR2 KIAA1012 remains conserved in mammals but no NEUR2 debris remains.) Newropsins retain many key attributes of GPCR signaling proteins and indeed opsins such as the seven transmembrane arrangement, Schiff base lysine, counterion tyrosine, amino terminal glycosylation site, and disulfide but have a very odd replacement of the G-protein binding site DRY with (invariantly conserved) VCC.

This motif must be an ancient derived feature that followed the gene duplication event with neuropsin since the much older DRY could not plausibly have re-evolved in neuropsin from VCC. Newropsins very likely link covalently via their orthologous Schiff base lysine with a retinal and interact with light according to some action spectrum. The VCC motif has been conserved over billions of years of branch length so cannot reflect simple loss of DRY functionality; however its signaling capabilities if any are unclear.

Below, conserved residues are shown for NEUR2 relative to human neuropsin (which repesents that family accurately). Newropsin orthologs are rather rapidly diverging, especially in teleost fish. Transmembrane domains on newropsins were assigned by homology to neuropsin (taken from SwissProt ab initio annotation consistent with experimentally determined bovine rod rhodopsin). Newropsin is relatively truncated amino terminally but very extended in a highly variable manner carboxy terminally. That extension would lie in the cytoplasm and possibly be removed endoproteolytically. The early glycosylation is present in all species but appears shifted distally by four residues in tetrapods relative to fish.

According to transcript annotations, newropsin is expressed in zebrafish anterior segment (minus lens), fish brain and testes (Pimephales and Oncorhynchus), embryo, oviduct and fat body (Xenopus). These, while familiar sites from other opsins, provide only meagre constraints on possible newropsin functionality and association with photoreceptive tissues.

The intronation pattern is not a perfect match to neuropsin as might be expected. Some of the difference is lineage-specific, such as a gain in zebrafish, but other differences may be much older. Unless homologs have been retained recognizably in earlier diverging species, it won't be feasible to date the original gene duplication. No NEUR2 could be located in lamprey, tunicate, or lancelet.

Newropsin is further evidence that the neuropsin/peropsin/rgropsin group played a much greater role in ancestral vertebrate photoreception (which persisted into contemporary species), roles which were lost in stem mammals. That is quite similar to the ciliary opsin story. Overall mammals have retained less than half (7 of 17) of the vertebrate opsin repertoire. Such widespread gene loss is fully consistent with an old inference of a nocturnal era during which no selective pressure existed to maintain these photoreceptors.

Opsin NEWR.png 


position     ...................................................................................................1.........1.........1.........1.........1.........1.........1.........1........1
position     .........1.........2.........3.........4.........5.........6.........7.........8.........9.........0.........1.........2.........3.........4.........5.........6.........7........7
position     12345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
excMemCy     eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMcccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeee
keyResid     ...GLC.........glc.glc.................................................................................diS..cIon.................DRY?..............................................
NEUR2_galG                MDPSFANST-FQSKITEAADIVVGTCYMVFGICSLCGNSILLYISYKKKHLLKPAEYFIINLAISDLAMTLTLYPLAVTSSLSHRWLYGKHICLFYAFCGLFFGICSLSTLTLLSVVCCLKICFPAYGNRFRRKHGQILIACAWTYAAIFACSPLAHWGEYGEEPY
NEUR2_anoC                MESYFANTT-FHSKITEAADVIVGVFYIVFGICSFCGNSILLYVSYKKKNLLKPAEYFMINLAISDLGMTLTLYPLAVTSSLAHRWLFGQQVCLFYAFCGVFFGVCSLTTLTLLSIVCCLKICFPVYGNRFRPGHGWILIACAWVYAAIFAFSPLAHWGEYGAEPY
NEUR2_xenT                MGNKSDASA-FYSSISETDDIVLGVLYSVFGLLSLSGNSMLLLVAYRKRSILKPAEFFIVNLSISDLGMTGTLFPLAIPSLFAHRWLFDKVTCNYYAFCGMLFGLCSLTNLTVLSSVCCLKVCYPAYGNKFSTAHSRILLLGIWAYAGLFATAPLADWGKYGPEPY
NEUR2_danR                MGNVSKTAL-FMSTISRQHDILMGSLYSVFFVLSLLGNGMLLFVAYRKRSSLKPAEFFVVNLSVSDLGMTLSLFPLAIPSALAHRWLFGEITCLCYAVCGVLFGLCSLTNLTALSSVCCLKVCFPNYGNKFSSSHACVMVIGVWCYASVFAVGPLVHWGSFGPEPY
NEUR2_pimP                MGNVSETAL-FVSTISRQHDILMGSLYSVFCVLSLLGNGMLLFVAYRKRSSLKPAEFFVINLSVSDLGMTLSLFPLAIPSALAHRWLFGEVVCLCYAVCGVLFGLCSLTNLTALSSVCCLKVCCPNYGNKFSSNHACVMVIGVWCYASVFAVGPLIRWGSFAPEPY
NEUR2_tetN                MGNASDTSDAFNSKISKEHDFLIGSIYSVFCVLSLMGNCILLLVAHHKRSTLKPAEFFIVNLSISDLGMTLTLFPLAIPSSFSHRWLFGEIACQLYATCGVLFGLCSLTNLTVLSSVCCLKVCLPNLGSKFSSSHARLLVAGVWGYASVFAVGPLVQWGHYGPEPY
NEUR2_takR                MGNASEASDIFLSKISKEHDILIGSIYSVFGLLSLAGNCILLLVAYHKRSMLKPAEFFIINLSISDLGMTLTLFPLAIPSSFSHRWLFGEITCQLYAMCGVLFGLCSLTNLTALSLVCCLKVCFPNHGSRFSSSHARLLVVGVWCYASVFAVGPLVQWGHYGPEPY
NEUR2_gasA                MGNASDTSAVFASTISKERDILMGSLYSVFGVLSLVGNCILLLVAYHKRSTLKPAEFFIINLSISDLGMTLSLFPLAIPSAFKHRWLFGELTCQLYAMCGVLFGLCSLTNLTALSFVCCLKVCFPNHGNRFSSSHARLLVVAVWGYASVFAVGPLARWGRYSPEPY
NEUR2_oryL                MGNVSDTSSLFASSISREHDILMGSLYSVFGLLSLSGNSMLLLVAYRKRSILKPAEFFIVNLSISDLGMTGTLFPLAIPSLFAHRWLFGEITCQLYAMCGVLFGLSSLTNLTALSLVCCLKVCFPNHGNKFSFSHARLLVAGVWCYASVFAVGPLARWGRYSAEPY
NEUR2_calM                                              GILSLVGNSVLLFVAYRKRQILKPAEYFVANLAVSDISMTVTLLPLAISSNFSHRWLFVSKPCMYYGFCSMLFGICSLTNLTVLSTVCCMKVCFPAYMSVVMIV-MFLLAWSPYSIVCLWASFGNPKLIPPAMAII
NEUR1_homSa   MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPF
NEUR1_canFa   MALNHTARPQDERLPHYLREGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPF
NEUR1_musMu   MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPF
NEUR1_loxAf   MTLNHTAPPQDDRLPQYLQDGDPFTSKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSCRRKKKLRPAEIMTINLAVCDLGISVVGKPFVIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPF
NEUR1_monDo   MALNHSVSPQDDYIPHYLRDGDPFASKLSWEADLVAGFYLTIIGVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGISVVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSYGTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPF
NEUR1_ornAn    MTNYSAPQLGDYLPHYLREGDPFVSKLSWEADLVAGVYLVIIGVLSTLGNGYVIYMSSRRKKKLRPAEIMTVNLAVCDLGISVVGKPFTIVSCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSYGTWLKRHHAYICLAIIWAYASFWATMPLVGLGNYAPEPF
NEUR1_calMi  MTAFDNSTALYSGYWLHDSLHGDPFVSKLSWEADIISACYLIVTGLLSTLGNGYVIYLSITQKRKLKPPEILITNLAISDFGMSVGGQPFLIISCFSHRWIFGWVGCRWHGWAGFFFGCGSLITMTVVSLDRYLKICHLQYGSWLQRRHVFMSLAFIWFYAAFWATMPLVGWGNYAPEPF
NEUR1_galGa   MASDCNSSSQEEYLPHYMQQEDPFASKLSREADIIAGFYLTVIGILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGISVVGKPFSIISFFSHRWIFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLAYGTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPF
NEUR1_xenTr   MAGNSSYREESGYIPHYERDSDPFASKLSREADIFAGVYLMAIGILSTLGNGYVIYMACSRKKKLRPAEIMTINLAVCDLGISVTGKPFAIVSCFSHRWVFGWNACRWYGWAGFFFGCGSLITLTVVSLDRYLKICHLRYGTWLKRRHAFIALAVIWAYATLWATLPLVGVGNYAPEPF
NEUR1_danRe     MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVIGILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGISVSGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTIVSFDRYLKICHLRYGTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPF
NEUR1_takRu     MENDTSIPSGYVPHYLLRGDPFASKLSKEADIVAAFYILVIGVLSATGNGYVIYQTIKRKTKLKPPEFMTLNLAVFDFGISVTGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTIVSLDRYLKICHLRYGTWFKRHHAFLCLVFTWLYAAFWATMPVVGWGNYAPEPF
NEUR1_tetNi     MENETWTHSSYVPHYLLRGDPFASRLSKEADIVAALYICIIGLMSATGNGYVLYMTFKRKTKLKPPELMTLNLAIFDFGISVTGKPFFIVSSLSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRYGAWLKRHHAFLCLASVWAYAAFWATMPLVGWGSYAPEPF
NEUR1_gasAc     MDNETRSHPSYVPHYLLRGDPFASRLSKEADIVAAFYIFIIGVMSATGNGYVLYMTFKRKTKLKPPELMTVNLAIFDFGISVTGKPFFIVSSLSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRYGTWLKRHHAFVCLALVWAYAAFWATMPLVGWGSYAPEPF
NEUR1_oryLa     MENETWTHPSYIPHYLLRGDPFASRLSKEADIIAAFYICIIGIMSATGNGYVIYMTIKRKSKLKPPELMTVNLAVFDFGISVTGKPFFVVSSFAHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRYGTWLKRQHAFLCLVFVWMYAAFWATMPLVGWGNYAPEPF
NEUR1_pimPr     MENTSWPHSSYVPHYLLRGDPFASRLSKEADIVAAFYILIIGIMSATGNGYVIYMTIKRKSKLKPPELMTVNLAVFDFGISVTGKPFFVVSSFSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRYGTWLKRQHIFLCLVFVWIYAAFWATMPLVGWGSYAPEPF
NEUR1_anoCa       MEQGQNISSQDDNQQEEDPFASKLSVEADIVAGVYLLVIGILSTLGNGYVIYMSTQRKKKLKPAEIMTVNLAVCDLGISVVGKPFSIIAFFSHRWIFGWSGCRWYGWAGFFFGIGSLITMTAVSLDRYFKICHLSYGTWLKRHHVFICLGIIWSYAAFWATIPFAGFGNYAPEPF

position    1.........1.........2.........2.........2.........2.........2.........2.........2.........2.........2.........2.........3........3..........3.........3.........3.........3.....3
position    8.........9.........0.........1.........2.........3.........4.........5.........6.........7.........8.........9.........0........1..........2.........3.........4.........5.....6
position    012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456
excMemCy    eeeeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMccccccccccccccccccccccccccccccccccMMMMMMMMMMMMMMMMMMMMMeeeeeeeeeeeeeeeMMMMMMMMMMMMMMMMMMMMMcccccccccccccccccccccccccccccccccccccccccccccc*
keyResid    .diS................................................................................................................K............................................................
NEUR2_galG  GTACCIDWQSTNVDVMSMSYTVVLFVLCFILPCGVIVTSYSLILVTVKESRKAVEQHVSGPTRINNVQTITAKLSIAVCIGFFAAWSPYAIIAMWAAFGSIDKIPPLAFAIPAVFAKSSTLYNPIIHLLLKPNFRSNIAKDFTVIQQLCVR---CCFCVKELQ--TYRSTFNTGLRTFKG
NEUR2_anoC  GTACCIDWRISNMKKTAMSYTTALFVFCYIIPCGIIITSYTLILITVKDSRKAVEQHALGPTRMSSVHTITAKLSIAVCIGFFVAWSPYAIIAMWAAFGSIDMIPPLAFAVPAVFAKSSTLYNPAMYLFLKPNFRSTIAKDLTVLHRLCLK---SCFCPRGMQNCSYRSALEAPLKSFKG
NEUR2_xenT  GTACCLDWEASYRERKALSYTISLFVFCYLIPSSLIFISYTLIFVTVKGARRAVQQHLSPQAKGSSIHSLIIKLSIAVCIGFLIAWTPYAIVAMMAAFGDPTKIPSLVFALAAAFAKSSTIYNPVVYLLLKPNFLNVVTKDLTLFQTMCAV---VCGWCR-----TPAVKTPCPHKDLKT
NEUR2_danR  GTACCINWYTPSHDALAMSYIISLFIFCYVVPCTIIILSYTFILVTVRGSQQAVQQHVSPQTKVTNAHALIVKLSVAVCIGFLTAWSPYAIVAMWAAFSANEQVPPTAFALAAIMAKSSTIYNPMVYLLFKPNFRKSLSQDTQMFRHRICLSHSKASPSPGMKDQERQSSQQCNNKDGSI
NEUR2_pimP  GTACCINWYIPSHDALAMSYIISLFIFCYVVPCTIIILSYTFILLRVRGSRQAVQKHVSPKTKETNAHTLIVKLSVAVCIGFVTAWSPYAVVAMWAAFSANEPVPPTAFALAAILAKSSTIYNPMVYLLFKPNFRKILSQDTQNIRHRMCVSHSKASPTPEIK---AQSSQQC--KDATI
NEUR2_tetN  GTACCINWQAPNHELSSLSYIVCLFLFCYVLPCAIIILSYTCILMTVRGSRQAIQQHVSPQTKTANAHALIVKLSVAVCIGFLGAWSPYAVVAMWASFGDATWVPPDAFAIAAILAKSSTIYNPLVYLLCKPNFRECLYKDTSTLRQRIY----RGSPLSGPRDRSGGVTQR--HKDLSV
NEUR2_takR  GTACCIDWRAPNHELSSLSYIVCLFFFCYVLPCATIILSYTCILMTVRGSRQAIQQHVSPQTKTANAHSLIVKLSVAVCIGFLGAWSPYAIVAMWAAFGDATWVPPDAFAIAAILAKSSTIYNPVVYLLCKPNFRECLYKDTSTLRQRIY----RGSPQSEPRERFGGTSQR--HKDLSI
NEUR2_gasA  GTACCIDWHAPNHELAALSYIVCLFVFCYALPCATIFLSYTFILLTVRGSRQAVQQHVSPQTKTTNTHALIVKLSVAVCIGFLGAWTPYAVVAIWAAFGDATLVPPDAFALAAMFAKSSTIYNPVVYLLCKPNFRACLYRDTTLLRQRIY----RGSPRSEPKAHFGSTSQR--NKDMSV
NEUR2_calM  APLFAKSSTFYNPCIYVISYTMTVIAVNFVVPLSVMFFCYYNV   
NEUR2_oryL  GTACCIDWHAPNHELWALSYILCLFIFCYALPCTIIFLSYAFILLTVRGSRQAVQQHVSPQTKTTNAHTLIVKLSVAVCIGFLGAWTPYAVIAMWAAFGDATQVPPTAFALAAVFAKSSTIYNPMVYLLCKPNFRECLCRDTSLLRHMIY----RGSP--QPQERFGSDSRR--NKDITA
NEUR1_homSa GTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKA-TKKKSLEGFRLHTVT-TVRKSSAVLEIHEEV    
NEUR1_calJa GTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKA-TKKKSLEDFRLHTVT-TVRKSSAVLEIHEEV    
NEUR1_canFa GTSCTLDWWLAQASLGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGRLKA-TKKKSLEDFRLNTVT-TVRKSSAVLEIHQEV    
NEUR1_musMu GTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQAGGLRG-TKKKSLEDFRLHTVT-TVRKSSAVLEIHQEV    
NEUR1_loxAf GTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHMLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRA-TKKKSLEGFRLHTVT-TVKKSSAVLEVHQEV    
NEUR1_monDo GTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKA-AKKESLRTYRLHTVT-TVRRSSAVLEIHQEV    
NEUR1_ornAn GTSCTLDWWLAQASVAGQAFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPIQFSVVPTLLAKSAAMYNPIIYQVIDCRISCCRLGGPKT-GKKESLKNSRSHSMS-TIRKPSAVSGPHQEV    
NEUR1_calMi GTSCTLDWWLARVSVSGLIFVLTILFFCLLLPIIIIVFSYIKIIAKVKSSAKEVAHFDSRIQNHHSLEMNLTK                                                                                                          
NEUR1_galGa GTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGQPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCRSGGPKTLQKKSSLKESRMYTIS-SHRDSAALSGTQLEV    
NEUR1_xenTr GTTCTLDWWLAQASVKGQIFVLSMLFFCLLFPTMVIVFSYAKIIAKVKSSAKEVAHFDTRNQNNHTLEIKLTKVAMLICAGFLIAWFPYAVVSVWSAFGQPDSIPIELSVVPTMMAKSASMYNPIIYQVIDCKPACCKK------DKSLQNTTSRVYTIS-TFRKSTTSAR         
NEUR1_danRe GTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTKVAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSRFYTISGSIKQR-PGDEASIEI    
NEUR1_takRu GTSCTLDWWLAQASVSGQSFVMCMLIFCLVLPTGVIVFSYVMIILQVKSSAQEVSHFDTQNKNKHHLEMKLTKVAMLICAGFLIAWIPYAVVSVVSAFGDPDSVPISISVVPTLLAKSSAMYNPIIYQVIDCKKNCAKLSCFQAWSKRKHYKTSRFYSISASMKKR-PANEVPTEI    
NEUR1_tetNi GTSCTLDWWLAQASVSGQSFVMAILFFCLILPTGIIVFSYVMIIFKVKSSAKEISHFDARIRNSHDLEIKLTKVAMLICAGFLIAWIPYAVVSVISAFGEPDSVPIPVSVIPTLLAKSSAMYNPIIYQVVDVKTSCTNFSCCKALKERIHFRKSRLYTISGSLRDPLPPKEAHIEM    
NEUR1_gasAc GTACTLDWWLAQASVSGQSFVMAILFFCLVLPTGIIVFSYIMIIFKVKSSAKEISHFDARIKNSHSLEIKLTKVAMLICAGFLIAWIPYAVVSVVSAFGEPDSVPIPVSVIPTLLAKSSAMYNPIIYQVADLKTSCTSSSCCKALKERVLFRKARLYTISGSLRDTLPPKEAHIEM    
NEUR1_oryLa GTSCTLDWWLAQASVSGQSFVVAILFFCLVLPAGIIVFSYVMIIFKVKSSAKEISNFDARIKNSHNLEIKLTKVAMLICAGFLIAWIPYAVVSVVSAFGEPDSVPISVSVIPTLLAKSSAMYNPIIYQVLDLKNSCMKSSCFKGLKKPRHFRKSRFYTISGSVKDNTTAKEAQIEM    
NEUR1_pimPr GTSCTLDWWLAQASVSGQSFVMSILFFCLVLPAGIIVFSYVMIICKVKSSSKEVSSFDARIKNSHTLEIKLTKVAMLICAGFLIAWIPYAVVSVVSAFGEPDSIPIPVSVIPTLLAKSSAMYNPIIYQLVDLKNSC-STCCAKVIRKRTHFRNSRFYTISGSLKDTAPAKEAHIEI    
NEUR1_anoCa GTSCTLDWWLAQGSVAGQAFILNILFFCLVLPTAVIMFCYVKIIAKVQSSTKEVAHYDTRIQNQHVLEMKLTKVAMLICAGFMFAWIPYAVVSVWSAFGRPDSVPIKVSVIPTLLAKSAAMYNPVIYQVIDCKSACCRPGNLQPLQKKNSR

Neuropsin (NEUR1 compared to newtopsin (NEUR3)

A third paralogous family NEUR3 was reported in July 2008 and characterized by syntenic relations and expression in chicken. However in chicken, NEUR1 actually is most abundant of the three paralogs in developing and early posthatch neural retina, notably in differentiating ganglion cells and amacrine cells.

Recoverable sequences range from lamprey to shark to fish (where a further lineage-specific tandem duplication has occured) to frog to sauropsids, with the gene again lost in all mammals including platypus. This locus exhibits an ancestral fusion of exon 2-3 relative to neuropsin and newropsin. The tandem duplication in rayfinned fish could easily be mistaken for a whole genome duplication effect -- however there is no sign of such an event for any of the 3 paralogs in any of the 5 fish with assembled genomes.

The NEUR3 group could possibly signal through heteromeric G protein. The DRY motif is a bit unusual, consisting of V/I R F/Y whereas the YNPxIY x aliphatic is fairly conventional. The Schiff base K is preserved in both NEUR3a and NEUR3b sequences.

The other oddity is NEUR1 and NEUR3 are on the same chromosome for all species examinable. They are separated by a million or so bp as well as other coding genes. This possibly represents an old tandem duplication that experienced subsequent rearrangements. On the whole, synteny has been quite well preserved for all NEUR genes: 

NEUR3_galGal    +CRISP   +RHAG  .....  +MUT  -NEUR3   .....  +CDC5L  -SUPT3H  +RUNX2
NEUR3_anoCar    -CRISP2  +RHAG  .....  +Mut  -NEUR3   .....  +CDC5L  -SUPT3H  +RUNX2
NEUR3_xenTro    -CRISP2  -RHAG  -PPHLN +MUT  -NEUR3   .....  +CDC5L  .....    .....
NEUR3a_danRer   +XRN2  -TSTA3  +MGST3  +MUT  -NEUR3a  -NEUR3b -NAPB  +DNMT3A  .....
NEUR3a_tetNig   .....   .....   .....  +MUT  -NEUR3a  -NEUR3b .....   .....   +RUNX2
                  
NEUR1_galGal    .....  +TNFRSF2  +CD2AP  +GPR111  +NEUR1    .....  +MRPL19  .....  .....
NEUR1_anoCar  -GPR111  -TNFRSF2  +CD2AP    .....  +NEUR1  -SPATS1  +MRPL19  .....  +ITSN2
NEUR1_xenTro  .....    +TNFRSF21 +CD2AP  +GPR111  +NEUR1  -PTCHD1  +MRPL19  .....  +ITSN2
NEUR1_danRer  +GPR111  -TNFRSF21 +CD2AP  .....    +NEUR1  +CNIH2    -LBR    -ENAH  +ITSN2
                   
NEUR2_galGal  -DSC1  +DSG2  +TTR  -B4GALT6  -NEUR2  -K1012  +RNF138  .....  .....
NEUR2_anoCar  -DSC1  +DSG2  +TTR  -B4GALT6  -NEUR2  -K1012  +RNF138  .....  .....

NeurAll.jpg

A proposed revised terminology for this family follows. Note NEUR2 and NEUR3 will never receive official HGCN nomenclature because (like thousands of amniote ancestral genes) they are absent in human and mouse. Here lower case a and b are used in the case of lineage-specific duplications, with a reserved for the copy with higher blastp score to human (or, if absent, nearest species). 

Gene   Protein    HGCN  Synonyms  Lineage-specific duplicate  DRY YNPxxY K Accessions 
            
NEUR1  neuropsin  OPN5  cOpn5m    NEUR1a/b cephalochordate    DRY YNPIIY K NM_181744 NM_001130743
NEUR2  newropsin  ----  cOpn5L1                               VCC YNPxIY K           XM_419178
NEUR3  newtopsin  ----  cOpn5L2   NEUR3a/b actinopterygii     vRf YNPxIY K           XM_420056

Curated Set of 52 deuterostome neuropsins

>NEUR1_homSap OPN5 1_6 43 0 1  2_6 40 2 1  3_6 57 2 1  4_6 112 2 0  5_6 81 0 2  6_6 19 1 0
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR1_panTro
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKETKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR1_gorGor
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 12 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 YASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIPSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGKPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFQ 2
1 LHTVTTVRKSSAVLEIHEEv* 0

>NEUR1_ponPyg
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGSPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR1_nomLeu
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTSVRKSSAVLEIHEEv* 0

>NEUR1_macMul
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADIVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR1_papHam
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADIVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEEv* 0

>NEUR1_calJac
0 MALNHTSLPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHEEV* 0

>NEUR1_tarSyr
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VIGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKGVAHFDSRIHTSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHEEv* 0

>NEUR1_otoGar
0 MALNHTALPQDELRPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 WTMMPLVGLEDYVPEPFTSCTLDWWLAQSLGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKTTKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR1_micMur
0 MALNHTVLPQDERLPHYLRDGDPFASKLSWEADLVAGFYLIII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2  1
2 GVWLKRKHAYICLALIWAYVSFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDHR 2
1 LHTVTAVRKSSAVLEIHQEv* 0

>NEUR1_tupBel
0 MALNHTALPQDESLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHVLEMKLTK 0
0  2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR1_musMus
0 MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQAGGLRGTKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR1_ratNor
0 MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 gVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPNSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQTGGLRATKKKSLEDFR 2
1 LHTVTAVRKSSAVLEIHPEv* 0

>NEUR1_speTri
0 MALNHTALPQDEHLPHYLRDEDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAFICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFINILFFCLLLPTAVIEFSYVKIIAKVKSSSEEVAHFDSRIHSSHV 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPSLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTAVRKSSAVVEIHQEv* 0

>NEUR1_dipOrd
0 MAFNHTAGTQGQGLPHYLPEEDPFTSKLSWEADIVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASLAGQVFILNILFFCLLLPTSVIVFSYVKIIAKVKSSSKEVAHFDSRIPSSHVLEMKLTK 0
0  2
1  * 0

>NEUR1_cavPor
0 MALNHTAPPQNEHLPRYLQDEDPFVSKLSWEADLVAGFYLTII 1
2 GILSTVGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 vVGKPFTIISCFRHRWVFGWIGCRWYGWAGFFFGCGSLITMTVVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYVSFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASAGGQIFILHILFFCLLLPTAMIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDSRFACCQNAGLKATKKKSLEDFR 2
1 LHTVTTDRKSAVLEIHQEV* 0

>NEUR1_oryCun
0 MALNHTALPQDEHLPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRRHAYICLALIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFSCCRTSGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_ochPri
0 MALNDTALPQDEHLPHYFRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRLYGWADFFFGCGSLITMTAVSLDRYLK 1
2 GVWLKRRHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFSCCRTGGLKQTKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_canFam
0 MALNHTARPQDERLPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASLGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGRLKATKKKSLEDFR 2
1 LNTVTTVRKSSAVLEIhQEV* 0

>NEUR1_felCat
0 MALNHTAPPQDERLPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAE 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_bosTau
0 MALNHTAPPPDERRPPYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTVNLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GIWLKRKHAYICLAVIWAYAAFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEVHQEv* 0

>NEUR1_turTru
0  1
2 GILSTFGNGYVLYMSSRRKKKLKPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIPSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAVYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_susScr
0 MALNHTAPPPDERRPHYLREGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTVNLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYAAFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLVAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIRQEV* 0

>NEUR1_vicVic
0 MALNHTAPPPDERRPRHLRDGdPFASKLSWEADLVAGFYLTII 1
2 GILSTLGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWMFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2  0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHAVTTVRKSSAVLEIHQEV* 0

>NEUR1_equCab
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHVLEVKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEDFR 2
1 LHTVTTVRKSSAVLEIHQEV* 0

>NEUR1_myoLuc
0 MALNHTALPQDEGLPHYLQDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTVGNGYVLYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHTYICLAFIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQATVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKKVAHFDSRIH 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSSAMYNPIIYQVIDYKLACCQTGGLRATKKKSLENFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_pteVam
0 MALNHTVLPQDEHLPHYVRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTVGNGYVLYMSSRRKKKLRPAEIMTINLAICDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHGSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTSGLRATKKKSLEDFR 2
1 LHTITTVREASAVLEIHQEV* 0

>NEUR1_sorAra
0 MALNHTALPQDENLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLVVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPNSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQSGGLRATKKKSLDDFr 2
1 LHTVTTVRESSAVLEIHQEV* 0

>NEUR1_eriEur
0 MSLNQTALPQDEGLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 gVWLKRKHAYLCLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASLGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKKVAHFDSRIHSSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKANKKKSLKDYR 2
1 * 0

>NEUR1_loxAfr
0 MTLNHTAPPQDDRLPQYLQDGDPFTSKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSCRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFVIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASVGGQIFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRATKKKSLEGFR 2
1 LHTVTTVKKSSAVLEVHQEv* 0

>NEUR1_proCap
0 MTLNHTVLPEDDRLSHYLRDGDPFTSKLSWEADLVAGFYLTVI 1
2 GILSTCGNGYVLYMSYRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFIIISSFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2  0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSVPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCRTRGLRATKEKSLEGVR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_echTel
0 MALNHTAPPQDNSLPHYLRDGDPFVSKLSWEADLGAGFYLIII 1
2 GILSTFGNGYVLYMSYRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFSHRWVFGWTGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEIAHFDSRIHSSHMLEMKLTK 0
0  2
1 LHTITTVRKSSAVLEIHQEV* 0

>NEUR1_dasNov
0 MALNHTALPQDDRLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 gILSTFGNGYVLYMSSKRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRATKKKSLEDFR 2
1 LHTVTTVRESSAVLEVHQEV* 0

>NEUR1_choHof
0 MALNHTGLPQDDSLPHYFRDGDPFASKLSWEADLVAGFYLIII 1
2 GILSTFGNGYVLYMSSRRRKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAVIWAYASFWTTMPLLGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHMLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCRTGGLRATKKKSFEGFR 2
1 LHTVTTVRKSSAVLEIHQEv* 0

>NEUR1_monDom
0 MALNHSVSPQDDYIPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKAAKKESLRTYR 2
1 LHTVTTVRRSSAVLEIHQEv* 0

>NEUR1_macEug
0  1
2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSy 1
2 GTWLKRHHAYICLVIIWAYATFWATMPLAGLGNYAPEPFGTSCTLDWWLAQASVTGQTFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQSSHVLEMKLTK 0
0  2
1 RHTVSTIRKSSSVSETYQEV* 0

>NEUR1_ornAna
0 MTNYSAPQLGDYLPHYLREGDPFVSKLSWEADLVAGVYLVII 1
2 GVLSTLGNGYVIYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFTIVSCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAYICLAIIWAYASFWATMPLVGLGNYAPEPFGTSCTLDWWLAQASVAGQAFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPIQFSVVPTLLAKSAAMYNPIIYQVIDCRISCCRLGGPKTGKKESLKNSR 2
1 SHSMSTIRKPSAVSGPHQEV* 0

>NEUR1_galGal
0 MASDCNSSSQEEYLPHYMQQEDPFASKLSREADIIAGFYLTVI 1
2 GILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIISFFSHRWIFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLAY 1
2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCRSGGPKTLQKKSSLKESR 2
1 MYTISSHRDSAALSGTQLEV* 0

>NEUR1_taeGut
0 MASEYNNSSQEEYIPHYLQEEDPFASKLSREADIIAGFYLTII 1
2 GILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIISFFSHRWMFGWIGCCWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1
2 GTWLKRHHAFICLAIIWAYAMFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQVFVLSILFFCLLLPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0
0 VAMLICAGFLLAWIPYAVVSVWSAFGRPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIECRLACCRPGG 2
1 

>NEUR1_anoCar
0 MEQGQNISSQDDNQQEEDPFASKLSVEADIVAGVYLLVI 1
2 GILSTLGNGYVIYMSTQRKKKLKPAEIMTVNLAVCDLGIS 1
2 VVGKPFSIIAFFSHRWIFGWSGCRWYGWAGFFFGIGSLITMTAVSLDRYFKICHLSY 1
2 GTWLKRHHVFICLGIIWSYAAFWATIPFAGFGNYAPEPFGTSCTLDWWLAQGSVAGQAFILNILFFCLVLPTAVIMFCYVKIIAKVQSSTKEVAHYDTRIQNQHVLEMKLTK 0
0 VAMLICAGFMFAWIPYAVVSVWSAFGRPDSVPIKVSVIPTLLAKSAAMYNPVIYQVIDCKSACCRPGNLQPLQKKNSR 2
1 

>NEUR1_xenTro
0 MAGNSSYREESGYIPHYERDSDPFASKLSREADIFAGVYLMAI 1
2 GILSTLGNGYVIYMACSRKKKLRPAEIMTINLAVCDLGIS 1
2 VTGKPFAIVSCFSHRWVFGWNACRWYGWAGFFFGCGSLITLTVVSLDRYLKICHLRY 1
2 GTWLKRRHAFIALAVIWAYATLWATLPLVGVGNYAPEPFGTTCTLDWWLAQASVKGQIFVLSMLFFCLLFPTMVIVFSYAKIIAKVKSSAKEVAHFDTRNQNNHTLEIKLTK 0
0 VAMLICAGFLIAWFPYAVVSVWSAFGQPDSIPIELSVVPTMMAKSASMYNPIIYQVIDCKPACCKKDKSLQNTTSR 2
1 VYTISTFRKSTTSAR* 0

>NEUR1_danRer
0 MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVI 1
2 GILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGIS 1
2 VSGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTIVSFDRYLKICHLRY 1
2 gTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSR 2
1 FYTISGSIKQRPGDEASIEI* 0

>NEUR1_takRub
0 MENDTSIPSGYVPHYLLRGDPFASKLSKEADIVAAFYILVI 1
2 GVLSATGNGYVIYQTIKRKTKLKPPEFMTLNLAVFDFGIS 1
2 VTGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTIVSLDRYLKICHLRY 1
2 GTWFKRHHAFLCLVFTWLYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLAQASVSGQSFVMCMLIFCLVLPTGVIVFSYVMIiLQVKSSAQEVSHFDTQNKNKHHLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGDPDSVPISISVVPTLLAKSSAMYNPIIYQVIDCKKNCAKLSCFQAWSKRKHYKTSR 2
1 FYSISASMKKRPANEVPTEI* 0

>NEUR1_tetNig
0 MENETWTHSSYVPHYLLRGDPFASRLSKEADIVAALYICII 1
2 gLMSATGNGYVLYMTFKRKTKLKPPELMTLNLAIFDFGIS 1
2 VTGKPFFIVSSLSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GAWLKRHHAFLCLASVWAYAAFWATMPLVGWGSYAPEPFGTSCTLDWWLAQASVSGQSFVMAILFFCLILPTGIIVFSYVMIIFKVKSSAKEISHFDARIRNSHDLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVISAFGEPDSVPIPVSVIPTLLAKSSAMYNPIIYQVVDVKTSCTNFSCCKALKERIHFRKSR 2
1 LYTISGSLRDPLPPKEAHIEM* 0

>NEUR1_gasAcu
0 MDNETRSHPSYVPHYLLRGDPFASRLSKEADIVAAFYIFII 1
2 GVMSATGNGYVLYMTFKRKTKLKPPELMTVNLAIFDFGIS 1
2 VTGKPFFIVSSLSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GTWLKRHHAFVCLALVWAYAAFWATMPLVGWGSYAPEPFGTACTLDWWLAQASVSGQSFVMAILFFCLVLPTGIIVFSYIMIIFKVKSSAKEISHFDARIKNSHSLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGEPDSVPIPVSVIPTLLAKSSAMYNPIIYQVADLKTSCTSSSCCKALKERVLFRKAr 2
1 LYTISGSLRDTLPPKEAHIEM* 0

>NEUR1_oryLat
0 MENETWTHPSYIPHYLLRGDPFASRLSKEADIIAAFYICII 1
2 gIMSATGNGYVIYMTIKRKSKLKPPELMTVNLAVFDFGIS 1
2 VTGKPFFVVSSFAHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GTWLKRQHAFLCLVFVWMYAAFWATMPLVGWGNYAPEPFGTSCTLDWWLAQASVSGQSFVVAILFFCLVLPAGIIVFSYVMIIFKVKSSAKEISNFDARIKNSHNLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGEPDSVPISVSVIPTLLAKSSAMYNPIIYQVLDLKNSCMKSSCFKGLKKPRHFRKSR 2
1 FYTISGSVKDNTTAKEAQIEM* 0

>NEUR1_pimPro
0 MENTSWPHSSYVPHYLLRGDPFASRLSKEADIVAAFYILII 1
2 GIMSATGNGYVIYMTIKRKSKLKPPELMTVNLAVFDFGIS 1
2 VTGKPFFVVSSFSHRWLFGWEGCRFYGWAGFFFGCGSLITMTVVSLDRYLKICHLRY 1
2 GTWLKRQHIFLCLVFVWIYAAFWATMPLVGWGSYAPEPFGTSCTLDWWLAQASVSGQSFVMSILFFCLVLPAGIIVFSYVMIICKVKSSSKEVSSFDARIKNSHTLEIKLTK 0
0 VAMLICAGFLIAWIPYAVVSVVSAFGEPDSIPIPVSVIPTLLAKSSAMYNPIIYQLVDLKNSCSTCCAKVIRKRTHFRNSr 2
1 FYTISGSLKDTAPAKEAHIEI* 0

>NEUR1_calMil
0 MTAFDNSTALYSGYWLHDSLHGDPFVSKLSWEADIISACYLIVT 1
2 GLLSTLGNGYVIYLSITQKRKLKPPEILITNLAISDFGMS 1
2 VGGQPFLIISCFSHRWIFGWVGCRWHGWAGFFFGCGSLITMTVVSLDRYLKICHLQY 1
2 GSWLQRRHVFMSLAFIWFYAAFWATMPLVGWGNYAPEPFGTSCTLDWWLARVSVSGLIFVLTILFFCLLLPIIIIVFSYIKIIAKVKSSAKEVAHFDSRIQNHHSLEMNLTK 0
0  2
1  * 0

>NEUR1_petMar Petromyzon marinus (lamprey) frag
0 VAMMICAGFLLAWIPYAVVSVWSAFGAPDSVPVAVSMVPTMFAKSAAMYNPLIYQLLSRRGTGAHCCRCRKARGTLRRPR 2

>NEUR1a_braFlo from cDNA and genome chrUn  ++  176419943 176431046 11104 FE548698 
0 MATTPADRLDGLTPAGRGATTAETHADDFASKLSREADIVIGVYLILI 1
2 GTGAILGNGRVLWLSYRCRARLRPVEMFVVSLAAADVGLSLVGHPFSAASSLMGRWSFGSAGCTW 1
2 YGFVVFFLGIASIATMALMSIMRFMIVHKRY 1
2 GQYPSRRASCVLVAAAWLYGLFWACAPLA 1
2 GWSQYHPEPYGLSCSVDWGGFSRGAGGSSFIICMLLFCTAVPVVVMVTSYAAIFALYRQAQKGVVLNLQVNATFGGKRQRTER
0 IALAVCGGFLLAWLPYAVVGLWASVAGVDAVPLALASAAPLFAKSNSLWNPIIYLGMNERFR 2

>NEUR2b_braFlo from traces and genome chrUn  ++  187375671 187384042 8372 nearly identical chrUn ++ 32271780 32281075 9296
0 MATTPGLPLDGLAPTGRGVTAADTLDDDFASKLSREADIVIGVYLLLI 1
2 GTGSILGNGRVLWLSYRNWAKLRPVELFVVSLAVTDVGISVFGYPFAASSSLLGRWSFGSAGCTW 1
2 YGFTGFFFGLTSIANMALMSIMRFMIVYKGY 1
2 GPYPSRRATSGLIAAAWLYGLFWACAPLA 1
2 GWSQYHVEPFGLSCTVDWGSFSRDAGGMSFIICLLVFCVAIPVTAIMASYVAISAIYRQAKKSIAGHLQDNSAMCKKRNKLE 0
0 MALAVCGGFLLAWLPYAVVGLWSAVAGVDAVPLALASAAPLFAKSSSLWNPIIYLGMNDRFR 2
 
>NEUR1_strPur Strongylocentrotus purpuratus XM_001197837 CX694910 CX690664
0 MDVNAKWWTNETLRTRDQFSDDHYTSVLSYEGDIWAGVYLMFI 1
2 SLIAFIGNISVIVISLRKREKLKPIDLLTINLAIADFLICVVSYPLPMISAFRHR 0
0 WSFGKFGCVWYGFTSFLFAVGSMATLMVIALLRYAKLCRENV 1
2 DQYQSRPFVIKVIVAIWGFAFFTTAPPLFGWS 2
1 SYVPEPYHLSCTIDFADTSPSGLSYTYFTTIVVFFMPLMIIVLCYVAIARKMIHHNRRINVGHNAGRMLLEIRLLK 0
0 TACMITMAYTISWTPYAVIAMWVTYIPVNQIPDAFRILPAFCAKTSSVYNPIIYCIFNKSFRQDLSSLICCCACQCYTITINLDINSHAQQQFRRIEERR
DEVGTYKRRPLMICSNPFAWSRDFHETWRQRRIRGIHRNCRNNVRVENINVNFRRDTDMVELNAPTPAEIHRPELNTASTRSGARTKSMATHLPALEEVPSG
APQCSALLHNTPIPRSLQGTPLPYQPQPSTSDLHDEFLNPSVVSRNMCVIVVKPNIEEELSTD* 0

Curated Set of 11 vertebrate newropsins (Neur2)

>NEUR2_galGal Gallus gallus GenBank 5'UTR mistranslated as coding -B4GALT6 -NEUR2_galGal -KIAA1012
0 MDPSFANSTFQSKITEAADIVVGTCYMVF 1
2 GICSLCGNSILLYISYKKKHLLKPAEYFIINLAISDLAMTLTLYPLAVTSSLSHR 2
1 WLYGKHICLFYAFCGLFFGICSLSTLTLLSVVCCLKICFPAY 1
2 GNRFRRKHGQILIACAWTYAAIFACSPLAHWGEYGEEPYGTACCIDWQSTNVDVMSMSYTVVLFVLCFILPCGVIVTSYSLILVTVKESRKAVEQHVSGPTRINNVQTITAK 0
0 LSIAVCIGFFAAWSPYAIIAMWAAFGSIDKIPPLAFAIPAVFAKSSTLYNPIIHLLLKPNFRSNIAKDFTVIQQLCVRCCFCVKELQTYRSTFNTGLRTFKGKNESSCNALPIMEG
CSYFPSEKGSHTFECFKSYPNCFQERLSTMGCHLQDCESLENDLQVEVTQGSRNSMKVVEQEEKSTELDNLEITLEAVPVSCTFTDL* 0

>NEUR2_anoCar Anolis carolinensis
0 MESYFANTTFHSKITEAADVIVGVFYIVF 1
2 GICSFCGNSILLYVSYKKKNLLKPAEYFMINLAISDLGMTLTLYPLAVTSSLAHR 2
1 WLFGQQVCLFYAFCGVFFGVCSLTTLTLLSIVCCLKICFPVY 1
1 GNRFRPGHGWILIACAWVYAAIFAFSPLAHWGEYGAEPYGTACCIDWRISNMKKTAMSYTTALFVFCYIIPCGIIITSYTLILITVKDSRKAVEQHALGPTRMSSVHTITAK 0
0 LSIAVCIGFFVAWSPYAIIAMWAAFGSIDMIPPLAFAVPAVFAKSSTLYNPAMYLFLKPNFRSTIAKDLTVLHRLCLKSCFCPRGMQNCSYRSALEAPLKSFKGRNESSSNSVQIVGGCS
YFPCEKCHDPFECFKNYPKCCQGRLNVMDHTPRESISVENNMQSKTKHASEKYIKVVIRGEKNTDIDNLEITLEHIPTDIKFANL* 0

>NEUR2_xenTro Xenopus tropicalis abundant transcripts
0 MGNKSDASAFYSSISETDDIVLGVLYSVF 1
2 GLLSLSGNSMLLLVAYRKRSILKPAEFFIVNLSISDLGMTGTLFPLAIPSLFAHR 2
1 WLFDKVTCNYYAFCGMLFGLCSLTNLTVLSSVCCLKVCYPAY 1
2 GNKFSTAHSRILLLGIWAYAGLFATAPLADWGKYGPEPYGTACCLDWEASYRERKALSYTISLFVFCYLIPSSLIFISYTLIFVTVKGARRAVQQHLSPQAKGSSIHSLIIK 0
0 LSIAVCIGFLIAWTPYAIVAMMAAFGDPTKIPSLVFALAAAFAKSSTIYNPVVYLLLKPNFLNVVTKDLTLFQTMCAVVCGWCRTPAVKTPCPHKD
LKTTSKPPSSFKKSQGVCRNCVDTFECFRNYPRCCSVGNVDAAQPMAASLVRIPPANGAPQQTVQLVVSSSRTRSGVETVEVSTEAPMSDFIKDFI* 0

>NEUR2_danRer Danio rerio acquired new intron
0 MGNVSKTALFMSTISRQHDILMGSLYSVF 1
2 FVLSLLGNGMLLFVAYRKRSSLKPAEFFVVNLSVSDLGMTLSLFPLAIPSALAHR 2
1 WLFGEITCLCYAVCGVLFGLCSLTNLTALSSVCCLKVCFPNY 1
2 GNKFSSSHACVMVIGVWCYASVFAVGPLVHWGSFGPEPYGTACCINW 2
1 YTPSHDALAMSYIISLFIFCYVVPCTIIILSYTFILVTVRGSQQAVQQHVSPQTKVTNAHALIVK 0
0 LSVAVCIGFLTAWSPYAIVAMWAAFSANEQVPPTAFALAAIMAKSSTIYNPMVYLLFKPNFRKSLSQDTQMFRHRICLSHSKASPSPGMKDQERQS
SQQCNNKDGSISTPFSSGQAESYGACHVYAEAGPHYQQISRQITARVLEGSVQSEIPVKQLTEKMQNDLL* 0

>NEUR2_tetNig Tetraodon nigroviridis gene mix
0 MGNASDTSDAFNSKISKEHDFLIGSIYSVF 1
2 CVLSLMGNCILLLVAHHKRSTLKPAEFFIVNLSISDLGMTLTLFPLAIPSSFSHR 2
1 WLFGEIACQLYATCGVLFGLCSLTNLTVLSSVCCLKVCLPNL 1
2 GSKFSSSHARLLVAGVWGYASVFAVGPLVQWGHYGPEPYGTACCINWQAPNHELSSLSYIVCLFLFCYVLPCAIIILSYTCILMTVRGSRQAIQQHVSPQTKTANAHALIVK 0
0 LSVAVCIGFLGAWSPYAVVAMWASFGDATWVPPDAFAIAAILAKSSTIYNPLVYLLCKPNFRECLYKDTSTLRQRIYRGSPLSGPRDRSGGVTQRHKDLSVSTR
LSNGQQDSYGTCLHCAEDAELGHVTGSRRTACILTGSTFTEVTLSQLSATPADLL* 0

>NEUR2_takRub Fugu rubripes
0 MGNASEASDIFLSKISKEHDILIGSIYSVF 1
2 GLLSLAGNCILLLVAYHKRSMLKPAEFFIINLSISDLGMTLTLFPLAIPSSFSHR 2
1 WLFGEITCQLYAMCGVLFGLCSLTNLTALSLVCCLKVCFPNH 1
2 GSRFSSSHARLLVVGVWCYASVFAVGPLVQWGHYGPEPYGTACCIDWRAPNHELSSLSYIVCLFFFCYVLPCATIILSYTCILMTVRGSRQAIQQHVSPQTKTANAHSLIVK 0
0 LSVAVCIGFLGAWSPYAIVAMWAAFGDATWVPPDAFAIAAILAKSSTIYNPVVYLLCKPNFRECLYKDTSTLRQRIYRGSPQSEPRERFGGTSQRHKDLSISTR
LSNGQQDSYGTCLHCADDAERGHVTTSQRTACILTGSTFTEVTVGQLSAAPADLL*

>NEUR2_gasAcu Gasterosteus aculeatus
0 MGNASDTSAVFASTISKERDILMGSLYSVF 1
2 GVLSLVGNCILLLVAYHKRSTLKPAEFFIINLSISDLGMTLSLFPLAIPSAFKHR 2
1 WLFGELTCQLYAMCGVLFGLCSLTNLTALSFVCCLKVCFPNH 1
2 GNRFSSSHARLLVVAVWGYASVFAVGPLARWGRYSPEPYGTACCIDWHAPNHELAALSYIVCLFVFCYALPCATIFLSYTFILLTVRGSRQAVQQHVSPQTKTTNTHALIVK 0
0 LSVAVCIGFLGAWTPYAVVAIWAAFGDATLVPPDAFALAAMFAKSSTIYNPVVYLLCKPNFRACLYRDTTLLRQRIYRGSPRSEPKAHFGSTSQRNKDMSVSVRSSNGQQDSYGACTENA
APCHVMTPQRTACILTESTNREVTVSRLADKPQADFL*

>NEUR2_oryLat Oryzias latipes 
0 MGNVSDTSSLFASSISREHDILMGSLYSVF 1
2 GLLSLSGNSMLLLVAYRKRSILKPAEFFIVNLSISDLGMTGTLFPLAIPSLFAHR 2
1 WLFGEITCQLYAMCGVLFGLSSLTNLTALSLVCCLKVCFPNH 1
2 GNKFSFSHARLLVAGVWCYASVFAVGPLARWGRYSAEPYGTACCIDWHAPNHELWALSYILCLFIFCYALPCTIIFLSYAFILLTVRGSRQAVQQHVSPQTKTTNAHTLIVK 0
0 LSVAVCIGFLGAWTPYAVIAMWAAFGDATQVPPTAFALAAVFAKSSTIYNPMVYLLCKPNFRECLCRDTSLLRHMIYRGSPQPQERFGSDSRRNKDITASTRFSNGQQESYGACLNCTEN
TGLCQLASPQNTACILTGSTYAEVTVQQLVDKQQPDFL* 0

>NEUR2_pimPro Pimephales promelas
0 MGNVSETALFVSTISRQHDILMGSLYSVF 1
2 CVLSLLGNGMLLFVAYRKRSSLKPAEFFVINLSVSDLGMTLSLFPLAIPSALAHR 2
1 WLFGEVVCLCYAVCGVLFGLCSLTNLTALSSVCCLKVCCPNY 1
2 GNKFSSNHACVMVIGVWCYASVFAVGPLIRWGSFAPEPYGTACCINWYIPSHDALAMSYIISLFIFCYVVPCTIIILSYTFILLRVRGSRQAVQKHVSPKTKETNAHTLIVK 0
0 LSVAVCIGFVTAWSPYAVVAMWAAFSANEPVPPTAFALAAILAKSSTIYNPMVYLLFKPNFRKILSQDTQNIRHRMCVSHSKASPTPEIK-AQSSQQCKDATISTPFSSGQAESYGTCHIYAEAEPHFQQISPQRTVRILEGIIQSEISVRHMTDRMQNDLL* 0

>NEUR2_oncMyk Oncorhynchus mykiss no glycosylation site, anomalous agreement with chicken
0 MGVLASIDDIAFLSNIPVAADITVAIVYAVF 1
2 GMCSLFSNSTLLYISYKKKHLLKPAEFFIINLAISDMSLTLSLYPMAITSSIYHR 2
1 WLFGKTVCLIYAFCGMLFGVCSLTTLTLLSMVCFVKVCYPLY 1
2 GNRFNAVHGRLLIACAWAWALVFACSPLAHWGEYGPEPYGTACCIDWRLSNLHPVARSYTAALFVLCYIVPCCVIVASYTGILMTVRASHKAMEHHEARQTKMSNIQDVIVK 0
0 LSVAVCIGFFAAWSPYAVVSMWAAFGHMDNIPPLAFAVPAMFAKSSTIYNPIIYLLLRPNFRRVMYRDLVSLCRAFLKGCLCSCSQGAVGKCHSHLVVRVSLQSFCRLPGHGQ
SCSPTSSARQALGESRGCTSPGEKCSDAFECFRHYPRGCHGGTNIPSSSARVYAPQDQLSTEPQLQSMTQKQMRKQEACHKKSLRATKHSKRTSEIDNLRINFEMVPGHAKVAWP* 0

>NEUR2_calMil frag
0 1
2 GILSLVGNSVLLFVAYRKRQILKPAEYFVANLAVSDISMTVTLLPLAISSNFSHR 2
1 WLFVSKpCMYYGFCSMLFGICSLTNLTVLSTVCCMKVCFPAY 1
2 0
0 MSVVMIVMFLLAWSPYSIVCLWASFGNPKLIPPAMAIIAPLFAKSSTFYNPCIYVISYTMTVIAVNFVVPLSVMFFCYYNV

Curated Set of 16 vertebrate newtopsins (NEUR3, NEUR3a/b)

>NEUR3_galGal Gallus gallus cOpn5L2 mRMA for Opsin 5-like 2 AB368183 chr3 XM_420056 CN231992 testis exon 2^3 rel NEUR1/2
0 MEEQYISKLHPVVDYGAGVFLLII 1
2 AILTILGNSAVLATAVKRSSLLKSPELLTVNLAVADIGMAISMYPLAIASAWNHAWLGGDASCIYYALMGFLFGVCSMMTLCAMAVIRFLVTNSSKSN 1
2 SNKISKNTVHILITFIWLYSLLWAILPLVGWGYYGPEPFGISCTIAWSKFHSSSNGFSFILSMFLLCTVLPALTIVACYLGIAWKVHKAYQEIQNINRIPHAAKLEKKLTL 0
0 MAVLISVGFLSAWTPYAAASFWSIFNSSDSLQPIVTLLPCLFAKSSTAYNPFIYYIFSKTFRHEIKQLQCCWGWRVHFFSADNSAENSVSMMWSGRDNIRLSPTAKVESQGAARH*

>NEUR3_taeGut Taeniopygia guttata ABQF01025032 
0 MEEQYISKLHPVVDYGAGVFLLII 1
2 AILTILGNSAVLATAVKRSSLLKPPELLTVNLAVADIGMALSMYPLAIASAWSHAWLGGDASCVYYALMGFLLGVCSMMTLCAMAVIRFLVTNSPKSN 1
2 sNKITKNTVCILIAFIWLYSLLWAILPLVGWGYYGPEPFGISCTIAWSKFHNSSNGFSFILSMFLLCTVLPALTIVACYLGIAWKVHKAYQEIQNIDRIPNAAKLEKKLTL 0
0 MAVLISVGFLSSWTPYAATSFWSIFNSSHSLQPVVTLLPCLFAKSSTAYNPFIYYVFSKTFRCEVKRLQCCCAWRVHYFSSDNSVENPLSTMWSGRDNIRLSAAPQVQNPGAAAP* 0

>NEUR3_anoCar Anolis carolinensis AAWZ01001057
0 MEEHYISKVHPVWDYGMGVFLLII 1
2 AILTILGNSMVLAVAVKRSSCLRSPELLTVNLAATDLGMGLSMYPLAIASAWNHAWLGGEATCIYYALMGFLFGVSSIMTLSAMAVIRFLVTFSSKPA 1
2 GHKINRKVMHICIMLIWAYAVLWAILPLLGWGHYGPEPFGTSCTIAWGQFHNSQKGFAFILSMFILCTFLPAITIIMCYLGIAWKFHKTHQEMQNLNRISSAAKLEKKLIL 0
0 VAVLISVGFLGAWTPYAIVSFWSVFHSSESIPYIVTLLPCLFAKSSTAYNPFIYYTFSKTFRHEVKHLRCYSGQRAQENMKNSINSNVSFMWHGGGNICLSTRQIEMREIPNQ* 0

>NEUR3_xenTro Xenopus tropicalis cdna ovary embryo
0 MEERYLSKLHPLVDFGSGVFLLLV 1
2 AILTVLGNCAVLATAVKCSSHLKAPDLLSINLAVADLGMAISMYPLAIASAWNHAWLGGDASCLYYALMGFFFGVSSMMTLTVMAIIRYRVTSSFKYS 1
2 GCTIEKKAVCILIMCIWLYALLWAVLPLLGWGRYGPEPFGTSCTIAWGDFHHSSNGFSFIISMFILCTISPAVTIVVCYSGIAWKLHKAYQEIKNQDKIPNSTKVEKKLTL 0
0 LAILVSFGFLISWTPYAAVSFWSLFHSSKYIPPVVSLLPCLFAKSSTAFNPMIYYAFSKTFRRKVKHLKCCCGWRVHFLQSENSVENPRVSVIWTGKENVMVSSVPKLMKGVPGTPTGTQ* 0

>NEUR3a_danRer Danio rerio
0 MDRYTSKLSPAVDYSAGTFLLVI 1
2 AILSILGNAAVLLTAAWRHSVLKAPELLTVNLAVTDIGMALSMYPLSIASAFNHAWIGGDPSCLYYGLMGMIFSVASIMTLAVMGLVRYLVTGNPPK 1
2 SGSKFRRKTISILIGVIWMYSLLWAVFPILGWGGYGPEPFGLACSVDWMGYQHSLNRSSFIMALAILCTLMPCVVILFSYSGIAWKLHKAYQSIQSNDNLPNSGAVERKVTL 0
0 MGILISTGFIVSWAPYVFVSLWTMFRSEGEDSVVPIVSLLPCLFAKCSTVYNPLVYYVFRKSFRREIHQIRICCFQGCWDAVSKMTRGDGPEETSGTHETDNI* 0

>NEUR3a_tetNig Tetraodon nigroviridis
0 MDDKYMSKLSPPVDLWAGIYLVVI 1
2 ALLSVLGNASVLFSASRRLTPLKAPELLTVNLAVTDIGMALSMYPLSIASAFNHAWMGGDTACLYYGLMGMIFSITSIMTLAVMGMIRYLVTGSPPR 1
2 SGVQFQKKTICVVICAIWLYACLWAAFPLLGWGSYGPEPFGTACSIDWTGYGDSLNNATFIVAMSVLCTFLPCLVIFFTYFGIAWKLHKAYKSIKSSDFQYASVERRITL 0
0 IAVLISVGFLGSWAPYGLVSLWSILKDSSSIPPQVSLLPCLFAKSSTVYNPVIYYIFSQSFKLEVQQLFLCC* 0

>NEUR3a_takRub Fugu rubripes
0 MDDKFTSKLSPAVDLWAGTYLVFI 1
2 ALLSVLGNASVLFSAGRRLSMLKAPELLTVNLAVTDIGMALSMYPLSIASAFNHAWMGGDASCLYYGLMGMIFSITSIMTLAVMGMIRFLVTGTPPR 1
2 SGIKFQKKTISVVISAIWLYACLWAVFPILGWGSYGPEPFGIACSVDWMGYGESLNNATFISTLSVLCTFLPYLVIVFTYFGIAWKLHRAYRSIKSSDIQYTNVERRITL 0
0 MAVMISSGFLIAWTPYVAVSFWSMRNSQRQGHMAPSVTLLPCLFAKSSTAYNPFIYFFFQRNTGHKLLPFHRHAFSCSDRADSSREGEKEE * 0

>NEUR3a_gasAcu Gasterosteus aculeatus
0 MEDKYVSKLSPAVDFWAGTYLIII 1
2 AVLSIFGNTAILVSAARRSGPLKAPELLTVNLAVTDLGMALSMYPLSIASAFNHAWIGGDASCLYYSLMGMIFSITSIVTLAVMGMVRYLVTGNPPR 1
2 SGLRLQRKTVSMVIGAVWLYSGLWALFPLLGWGSYGPEPFGLACSIDWSSYGESLNRSTFIMTLSVLCTFLPCLVIFFTYFGIAWKLRRAYQSIRSSDFQHGKVEQKITL 0
0 MAAMISSGFLFSWTPYVAVSLWSMFRSREHIPPLVALLPCLFAKSSTVYNPFIYFIFQRSSWRELLRLHRHLLCCWHRASPPAEGRRSQRGSEGGSWGGACESDDAFGLVHVMKSNATCQTISWA* 0

>NEUR3_calMil frag
2 AILSIFGNSVVLLVAAKKSSQLKPPELLTVNLAITDFCSAVTMYPLAVGSAWKHTWLGGDASCKYYGFMDFFFGIASIGTLTVMAIVRFLVTSTTQN 1
2               LVGLGLYGPEPFGISCTLAWSELYSSANSMSFIVASFILCTLLPTVIIATCYTGIAWKLHKAYHEIHNNQQVIISVKIDRKITQ 0

>NEUR3_petMar new exon frag
0 MAEQGEDDQFRSKLSPTADIAAGTFLLAV 1
2 AVLSLAGNGAVLGVAARRWAKLKAPELLSVNLALTDLGIAASIYPLAVASAWNHRWLGGQPVCTYYAFAGFFFGTASMGTLTAMAGVRYKGTSTQVH 1
2 VKQITKRAMLAVIVAVWAYALLWSCLPLLGWGR 2 1 YGVEPFGVSCTLAWAELQLTPGGVAFLYAMFVLCLLLPAIAIGLCYAGIVCKLRRAYREGRSKRRTPTARHVESRLTK 0

>NEUR3b_danRer Danio rerio
0 MDIYSSKLSSAVDYGIGAFLLLI 1
2 TILSILGNLMVLVMAYKRSNHMKPPELLSVNLAVTDLGAAVTMYPLAVASAWNHHWIGGDVSCVYYGLMGFLFGAASMMTLTIMAIVRFIVSLTLQSP 1
2 KEKISKRNAKILVATTWLYALLWAIFPLIGWGKYGPEPFGLSCTLDWRDMKEHSQSFVITIFLMNLILPAIIIVSCYCGIALRLYVTYKSMDDSNHVPNMIKMQRRLMV 0
0 IAVLISIGFVGCWAPYGIVSLWSIYRPGDSIPAEVSMLPCLFAKTSTVYNPFIYYIFSKTFKREVNQLSRFCGRSNICRPTDAKNRPENTIYLVCDVNKSKPGVEDLSLARSKENETQMLPNQDLHE* 0
 
>NEUR3b_tetNig assembly errs in exon 2 frameshift, used traces
0 MDMYTSALSPALDIGTGCYLLVI 1
2 AVLSFIGNLLVIITAVKKSSKMKPPELLCVNLAVTDLGAAVTMYPLSVASAWSHRWIGGDVTCVYYGLVGFLFEVASIMNLTVLAIVRFTVSLNLQSP 1
2 EEKISWKSVKIMCLLIWLYGVIWAMFPVLGWGRYGPEPFGISCSLAWGQMKNEGFSFVVAMFSFNLAVPALIIVSCYFGMAINLYFTHKKMVNTGNRIPAVIKLHRRLLR 0
0 IAVLISVGFLGSWAPYGLVSLWSILKDSSSIPPQVSLLPCLFAKSSTVYNPVIYYIFSQSFKLEVQQLFLCCLSFRSSRTNNCKSNESSIFMVSNGKNLTPALTQQNTSHAVIMN* 0
 
>NEUR3b_takRub 
0 MDIYSSTLSPALDIGTGCFILVV 1
2 GVLSIIGNLLVIITAVKRSSKMKPPELLCVNLAVTDLGAAVTMYPLSVASAWSHRWIGGDATCIYYGLVGFLFGVASIMNLTILAIVRFTVSLNLQSP 1
2 eEKITWKSVKIMCMWVWLYSIMWAMFPILGWGRYGPEPFGISCSLAWGQMKDEGFSFVVTIFSLNFAVPAVIIICCYFGIAIKLYFTYKKTVNTNQIPVIIKLHRRLLM 0
0 IAVLISVGFLGCWAPYGLVSLWSILKDSSSIPPEVSLLPCMFAKSSTVYNPIIYYMFSQSFKMEVQQLFLWCPSFEFCRTSSNNGNETTIYMVSTGKT* 0

>NEUR3b_gasAcu
0 MDIYASTLSPAVDVGAGCYLLFV 1
2 AVFSIVGNLLVLVMAVKRSSRMKPPELLSVNLAVTDLGAAVTMYPLAVASAWRHRWLGGDATCVYYAVAGFFFGLASIMSLTGLAIVRFIVSLNLQSP 1
2 NEKISWRKVKLLCACTWLYALAWAAFPFLGWGRYGPEPYGLSCSLAWGQMKHEGFSFVVSMFSLNLVLPCVIIAGCYFGIAFKLYFTYRKSNNNSNRLPNVVRRHRRLLA 0
0 IAVLISLGFVVCWSPYAVVSLWSIFHDSGSIPPEVSLLPCMFAKSSTVYNPLIYYIFSQSFRREVKQLWRHLGSTLCSVSNSVNDAAVSNTGKSN* 0

>NEUR3b_oryLat
0 MDIYASALSPALDIGTGCYLLVL 1
2 TVLSIIGNLLVVIMAFKRSSRMKPPELLSVNLALTDLGAAVFMYPLAVASAWSHHWLGGDVSCIYYGLAGFFFGSASVMNLTALAVVRFIVSLNLHSP 1
2 KEKVSWRKVKILCLWSWLYALIWALFPILGWGRYGPEPFGLSCSLAWGEMKQEGPSFVISLFSFNLVLPSVVIICCYFGIAMKLYFTYKKSANSNHVPNIIKLHRRLLIIA 0
0 ILISIGFIGCWTPYGLVSLWSIFNDSSKIPPEVSLLPCMFAKSSTVYNPMIYYFFSKSFQREVKQLSWLCVGSNPCHVSNSVNDNNIYMVSVNVKSKETRRETLQEITESRQ* 0
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