Marsupial phyloSNPs: Difference between revisions
Tomemerald (talk | contribs) m (→Case of XYLT1) |
Tomemerald (talk | contribs) m (→Case of XYLT1) |
||
Line 1,287: | Line 1,287: | ||
'''Known variations:''' Not a known disease gene. Natural human polymorphisms in XYLT1 have been observed, P325R, P766A, V8391 and R892Q but these do not include changes near the locus under consideration here A424. | '''Known variations:''' Not a known disease gene. Natural human polymorphisms in XYLT1 have been observed, P325R, P766A, V8391 and R892Q but these do not include changes near the locus under consideration here A424. | ||
'''Structural significance:''' The region enveloping the key residue has a weak 30% match encompassing residues 328-535 (thus including the A-->D residue at 424) that nonetheless is adequate for structural modeling to PDB structure [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=UAEKAVWX016&log%24=protalign&blast_rank=1&list_uids=112490721 2GAK] -- our residue is part of a short type I beta turn connecting strands 4 and 4' of the donor domain. The [http://www.jbc.org/cgi/pmidlookup?view=long&pmid=16829524 determined structure] match to residues 86-289 is a somewhat similar enzyme, 6-N-Acetylglucosaminyltransferase, a product of the GCNT1 gene. A glycine has replaced the alanine, showing the latter is not a deep invariant critical to this class of enzyme. | '''Structural significance:''' The region enveloping the key residue has a weak 30% match encompassing residues 328-535 (thus including the A-->D residue at 424) that nonetheless is adequate for structural modeling to PDB structure [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=UAEKAVWX016&log%24=protalign&blast_rank=1&list_uids=112490721 2GAK] -- our residue is part of a short type I beta turn connecting strands 4 and 4' of the donor Rossmann domain. The [http://www.jbc.org/cgi/pmidlookup?view=long&pmid=16829524 determined structure] match to residues 86-289 is a somewhat similar enzyme, 6-N-Acetylglucosaminyltransferase, a product of the GCNT1 gene. A glycine has replaced the alanine, showing the latter is not a deep invariant critical to this class of enzyme. | ||
[[Image:XYLT1align.jpg]] | [[Image:XYLT1align.jpg]] |
Revision as of 10:12, 26 February 2009
Introduction to Marsupial phyloSNPs
In this project, new genomic data from the Tasmanian devil (Sarcophilus harrisii), Tasmanian tiger (Thylacinus cynocephalus), and echidna (Tachyglossus aculeatus) are analyzed for significant changes at the protein coding level. The goal is to find single amino acid changes in one of these species at a highly invariant residue in a well-conserved exon in a gene with known or predictable tertiary structure. Such changes are thought to enrich for genetic changes with significant, adaptive biochemical or phenotypic consequences (1,2,3,4), in contrast to ordinary SNPs at positions of low conservation. Thus phyloSNPs are informative to the distinctive biology of the species carrying them and suggest a focus for subsequent experiment.
It is also of particular interest to determine the levels of variation within the Tasmanian devil population as a whole because the number of individuals have become low and possibly inbreed with adverse sequelae. For this it will be necessary to first determine sites of variation and then to genotype them across a large number of individuals.
Marsupial genomic and cDNA data to date has been quite limited compared to placental mammal. Yet as outgroup, metatheran animals provide important context to placentals and represent important context in understanding human protein evolution. The monotheres are inevitably limited by the paucity of extant species (basically platypus and echidna) and dim prospects for fossil DNA. Consequently echidna provides an important adjunct to the existing but incomplete platypus assembly. While extant birds and reptiles -- the preceding divergence node -- are abundant it must be remembered that a very considerable time elapsed (from 310 mry to 175 mry) prior to divergence of mammals with living representatives. This gap of 135 myr is comparable to the whole evolutionary record of theran mammals.
Assumed vertebrate phylogenetic tree
Marsupial relationships are taken from a 2009 paper establishing the mitochondrial genome sequences of the Tasmanian tiger (Thylacinus cynocephalus) and numbat (Myrmecobius fasciatus).
Newick tree that generates a marsupial-centric vertebrate phylogenetic tree: ((((((((((((sarHar,smiCra),myrFas),thyCyn),(macEug,triVul)),monDom), ((((loxAfr,proCap),echTel),(dasNov,choHof)), ((((((bosTau,turTru),susScr),vicPac),((equCab,(felCat,canFam)),(myoLuc,pteVam))),(eriEur,sorAra)), (((((((((homSap,panTro),gorGor),ponPyg),macMul),calJac),tarSyr),(micMur,otoGar)),tupBel), (((((musMus,ratNor),dipOrd),cavPor),speTri),(oryCun,ochPri)))))), (ornAna,tacAcu)), ((galGal,taeGut),anoCar)), xenTro), (((tetNig,takRub),(gasAcu,oryLap)),danRer)), calMil), petMar); Newick tree that generates the homo-centric vertebrate phylogenetic tree: ((((((((((((((((((homSap,panTro),gorGor),ponPyg),macMul),calJac),tarSyr),(micMur,otoGar)),tupBel), (((((musMus,ratNor),dipOrd),cavPor),speTri),(oryCun,ochPri))), (((((vicPac,susScr),turTru),bosTau),((equCab,(felCat,canFam)),(myoLuc,pteVam))),(eriEur,sorAra))), (((loxAfr,proCap),echTel),(dasNov,choHof))), (monDom,((macEug,triVul),(sarHar,thyCyn)))), (ornAna,tacAcu)), ((galGal,taeGut),anoCar)), xenTro), (((tetNig,takRub),(gasAcu,oryLap)),danRer)), calMil), petMar);
Phylo-sorting data
This tab-delimited table enables four different sort orders. These are needed because data can be missing from species in a manner that varies by gene, making data alignment difficult. Some alignment tools also lose input order, so that needs to be recovered. The ordering here flattens the phylogenetic tree by taking human (arbitrarily) at the top and resolving ambiguous situations (eg mouse, rat) by putting species with the best assemblies first.
The first two columns provide sort order number for the 44 species alignment at UCSC as phylogenetic and alphabetic order respectively. The third and fourth columns do this for a larger set of 53 species for which data is commonly available (notably in marsupials). The fifth column supplies the genSpp acronym and the sixth the Newick tree format syntax. These two columns by themselves will correctly draw the vertebrate phylogenetic tree in all online software without further editing. The final columns provide genus, species, and common name.
.. .. .. .. ...... (((((((((((( 46 10 54 10 anoCar )), Anolis carolinensis (lizard) 29 11 22 11 bosTau , Bos taurus (cow) 15 12 38 12 calJac ), Callithrix jacchus (marmoset) 62 54 61 13 calMil ), Callorhinchus milii (elephantfish) 32 13 28 14 canFam )),( Canis familiaris (dog) 23 14 46 15 cavPor ), Cavia porcellus (guinea_pig) 41 15 21 16 choHof )),(((((( Choloepus hoffmanni (sloth) 52 16 60 17 danRer )), Danio rerio (zebrafish) 40 17 20 18 dasNov , Dasypus novemcinctus (armadillo) 22 18 45 19 dipOrd ), Dipodomys ordii (kangaroo_rat) 39 19 19 20 echTel ),( Echinops telfairi (tenrec) 30 20 26 21 equCab ,( Equus caballus (horse) 35 21 31 22 eriEur , Erinaceus europaeus (hedgehog) 31 22 27 23 felCat , Felis catus (cat) 44 23 52 24 galGal , Gallus gallus (chicken) 50 24 58 25 gasAcu , Gasterosteus aculeatus (stickleback) 12 25 35 26 gorGor ), Gorilla gorilla (gorilla) 10 26 33 27 homSap , Homo sapiens (human) 37 27 17 28 loxAfr , Loxodonta africana (elephant) 58 56 14 29 macEug , Macropus eugenii (wallaby) 14 28 37 30 macMul ), Macaca mulatta (rhesus) 17 29 40 31 micMur , Microcebus murinus (mouse_lemur) 42 30 16 32 monDom ),(((( Monodelphis domestica (opossum) 20 31 43 33 musMus , Mus musculus (mouse) 33 32 29 34 myoLuc , Myotis lucifugus (microbat) 56 57 12 35 myrFas ), Myrmecobius fasciatus (numbat) 26 33 49 36 ochPri )))))),( Ochotona princeps (pika) 43 34 50 37 ornAna , Ornithorhynchus anatinus (platypus) 25 35 48 38 oryCun , Oryctolagus cuniculus (rabbit) 51 36 59 39 oryLap )), Oryzias latipes (medaka) 18 37 41 40 otoGar )), Otolemur garnettii (bushbaby) 11 38 34 41 panTro ), Pan troglodytes (chimp) 53 39 62 42 petMar ) Petromyzon marinus (lamprey) 13 40 36 43 ponPyg ), Pongo pygmaeus (orang) 38 41 18 44 proCap ), Procavia capensis (hyrax) 34 42 30 45 pteVam ))),( Pteropus vampyrus (macrobat) 21 43 44 46 ratNor ), Rattus norvegicus (rat) 54 58 10 47 sarHar , Sarcophilus harrisii (tasmanian_devil) 55 59 11 48 smiCra ), Sminthopsis crassicaudata (dunnart) 36 44 32 49 sorAra )),((((((((( Sorex araneus (shrew) 24 45 47 50 speTri ),( Spermophilus tridecemlineatus (squirrel) 60 60 24 51 susScr ), Sus scrofa (pig) 61 61 51 52 tacAcu )),(( Tachyglossus aculeatus (echidna) 45 46 53 53 taeGut ), Taeniopygia guttata (finch) 49 47 57 54 takRub ),( Takifugu rubripes (fugu) 16 48 39 55 tarSyr ),( Tarsius syrichta (tarsier) 48 49 56 56 tetNig , Tetraodon nigroviridis (pufferfish) 57 62 13 57 thyCyn ),( Thylacinus cynocephalus (tasmanian_tiger) 59 63 15 58 triVul )), Trichosurus vulpecula (bushytail_possum) 19 50 42 59 tupBel ),((((( Tupaia belangeri (tree_shrew) 28 51 23 60 turTru ), Tursiops truncatus (dolphin) 27 52 25 61 vicPac ),(( Vicugna pacos (lama) 47 53 55 62 xenTro ),((( Xenopus tropicalis (frog) 44 44 53 53 genSpp tree_syntax genus species common ph al ph al
Candidate analysis
The first issue is error within the primary reads themselves; the second is whether the default 454 Newbler assembler correctly identified overelapping reads and put them together properly to give exon-spanning reads. Those issues are discussed elsewhere -- here it is assumed the reads at the PSU blast site are correct, so the entire focus is on subsequent bioinformatics. In some cases that results in retrospective identification and correction of errors, notably introduced frameshifts that are far too common.
After thorough evaluation, candidates are given a final heuristic score based on awarding 0,1,or 2 points for the following 13 critera:
- the change is real: multiple reads support each of the two amino acid values
- quality coverage: the entire exon can be recovered from multiple reads without manual frameshift correction
- processed pseudogenes can be recognized by reads long enough at flanks to identify neighoring exons now adjacent (resp GT-AG splice donors)
- non-processed pseudogenes can be distinguished by recovery of additional exons of the gene with expected levels of conservation
- paralogs and internal repeats are readily distinguishable from the exon under stead
- phylogenetic depth: multiple marsupials, monotremes, all placental branches, fish, chondrichthyes, possibly lamprey available
- homoplasy: the reduced alphabet consists of a single amino acid with the exception of Sarcophilus
- appropriate character of the change in amino acid properties
- amenability to accurate rapid scoring in many individual animals
- interpretability of structural significance of change within 3D structure or characterized domain
- interpretability of functional role of overall gene and of region containing the amino acid change
- previous relevant publications, animal kockout models, known human ortholog disease SNPs
- plausible relevancy of the change to cancer or facial tumor
When scoring is finished, the dummy table below will be filled in with real data and genes will become sorted by highest overall score (or by preferred columns appropriate to specialized purposes).
..... valid cover psgen paral depth alpha AAcha popul struc funct pubmd tumor ERN2 1 1 1 1 1 1 1 1 1 1 1 1 12 MGAT5 1 1 1 1 1 1 1 1 1 1 1 1 12 ACTL6B 1 1 1 1 1 1 1 1 1 1 1 1 12 IPO7 1 1 1 1 1 1 1 1 1 1 1 1 12 PPFIA3 1 1 1 1 1 1 1 1 1 1 1 1 12 WDFY3 1 1 1 1 1 1 1 1 1 1 1 1 12 XYLT1 1 1 1 1 1 1 1 1 1 1 1 1 12 ATP4A 1 1 1 1 1 1 1 1 1 1 1 1 12 VPS72 1 1 1 1 1 1 1 1 1 1 1 1 12 ABCC1 1 1 1 1 1 1 1 1 1 1 1 1 12 ACOT12 1 1 1 1 1 1 1 1 1 1 1 1 12 FLI1 1 1 1 1 1 1 1 1 1 1 1 1 12 SPON1 1 1 1 1 1 1 1 1 1 1 1 1 12 ..... 13 13 13 13 13 13 13 13 13 13 13 13 .....
Case of ERN2
chr6_5971 ERN2 4 contig00001 length=355 numreads=5 KLPFTIPELVHASPCRSSDGVLYT .....................F.. ^ 15 R=3(75) H=2(50 Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5 and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in tasmanian devil (in this case, both individuals differ from Monodelphis by L->F), then differences between the two tasmanian devils (here one individual has R at position 15, the other has H), and finally the number of experimental reads that confirm the nucleotide difference and the sum of the quality scores. The sequences were assembled by Newbler (the official 454 assembler) which uses lower-case letters for less confident calls.
Pseudogene issues: ERN2 has not generated potentially confusing recent processed pseudogenes in mammals (lack of human, opossum or platypus genome Blat matches to ERN2 query). The variation observed here between individual tasmanian devils is implausibly an early stage in the loss of parent gene because of ERN2 functional essentiality; the exon cannot come from a decaying segmental duplication because coverage is high enough to also detect the main gene.
Paralog issues: The GeneSorter tool at UCSC shows a single significant full-length paralog in human, ERN1, also with 22 coding exons. The genes reside on different chromosomes but in regions with local homology of synteny. However this particular exon is a good match (3 differences out of 23), so there is potential for experimental difficulties in distinguishing them in short reads (including the following exon readily resolves them bioinformatically). In any event, at positions 15 and 20, ERN1 is identical at the amino acid level to ERN2. The gene duplication appears to have occured subsequent to amphioxus divergence earlier diverging metazoans are single-copy.
Homoplasy (recurrent mutation) issues: This exon is very conserved and does not exhibit repetitive sequence, compositional simplicity, or indels in any species in either paralog that could foster experimental error or alignment ambiguity. At position 15, the ancestral value is arginine in both paralogs. The G--> A transition to histidine in one individual is conservative under most circumstances (still basic) and arises from an arginine codon CpG hotspot conserved back to lamprey in 30 of 32 species with available data, yet histidine is not observed part of a reduced alphabet (ie R/H) at this position over many billions of years of branch length. Consequently R-->H is a significant change in this individual tasmanian devil.
Known variations: No human disease variants have been reported for either ERN2 or ERN1, probably attributable to essentiality. Site-specific mutation close to the exon here have been generated for K121P, D123P, W125A, and Q105E but only for ERN1. Naturally occuring coding SNPs in the human population relevent to the ERN2 exon are not known but low frequency alleles could emerge from the 1000 Genomes Project.
Side issues: a very ancient conserved leucine at position 21 appears to be transitioning to phenylalanine at marsupial node but has not been fixed, so settles out as L or F depending on lineage-sorting on each terminal marsupial leaf whereas placentals are all changed to phenylalanine (a phyloSNP caught in mid-air). While L and F might seem about the 'same' as amino acids, the branch length conservation totals say both are important but for different reasons: this is not a waffle codon nor reduced alphabet situation. This raises the question -- given the extreme conservation of this exon otherwise -- of whether the L-->F change at position 21 in both individuals has 'enabled' (made neutral or adaptive) an otherwise unfavorable R-->H change at position 15 in one individual.
Structural significance: By good fortune, the crystal structure of ERN1 (alternately called IRE1) has been published. The PDB 2HZ6 structure has good coverage of this particular exon. Consequently the marsupial ERN2 could be very accurately modelled and the structural effects of L-->F with or without R-->H computed by submission to online SwissProt modelling service.
Monodelphis ERN2 (key exon: sarHar2) aligned to human ERN1 luminal domain Expect = 5.8e-65 Identities = 109/180 (60%), Positives = 141/180 (78%) ERN2_monDom 1 PESLLFISTLDGSLHAVSKKTGDIQWTLKDDPIIQGPVYATEPAFLPDPSDGSLYILGEE 60 PE+LLF+STLDGSLHAVSK+TG I+WTLK+DP++Q P + EPAFLPDP+DGSLY LG + ERN1_homSap 8 PETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSK 67 ERN2_monDom 61 SKQGLMKLPFTIPELVHASPCHSSDGVFYTGRKQDTWFMVDPKSGKKQTMLSTETWDGLY 120 + +GL KLPFTIPELV ASPCRSSDG+LY G+KQD W+++D +G+KQ LS+ D L ERN1_homSap 68 NNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLC 127 ERN2_monDom 121 PSAPLLYIGRTQYTVTMYDPRSQALRWNTTYRGYSAPLLDHLPGYQVGHFTCSGEGLVVT 180 PS LLY+GRT+YT+TMYD +++ LRWN TY Y+A L + Y++ HF +G+GLVVT ERN1_homSap 128 PSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVT 187
Functional significance: A considerable amount is known about the paralog ERN1. Annotation transfer is likely applicable to ERN2. The two gene products differ primarily in expression -- ERN1 ubiquitious but ERN2 restricted to intestinal epithelial cells:
"The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1 or ERN1) and PKR-related ER kinase (PERK) are two type I transmembrane ER-localized protein kinase receptors that signal the UPR through a process that involves homodimerization and autophosphorylation... The monomer of the luminal domain comprises a unique fold of a triangular assembly of beta-sheet clusters. Structural analysis identified an extensive dimerization interface stabilized by hydrogen bonds and hydrophobic interactions... Mutations that disrupt the dimerization interface produced ERN1 protein that failed to either dimerize or activate the UPR upon ER stress."
"ERN1 is a type I transmembrane protein kinase receptor that also has a site-specific RNase activity that, upon activation, initiates a site-specific unconventional splicing reaction. The substrate for IRE1 RNase in metazoans is Xbp1 mRNA, which encodes a basic leucine zipper transcription factor of the ATF/CREB family. XBP1 controls expression of genes containing an X-box element or a UPR element in their promoter regions. The IRE1-mediated splicing reaction introduces into XBP1 an alternative C terminus, thereby generating an XBP1 molecule that is a more potent transcriptional activator. Therefore, activation of IRE1 and its RNase increases the transcription of genes encoding ER chaperones and folding catalysts... the ERN1 N-terminal luminal domain (NLD) functions as an ER stress sensor... under normal conditions IRE1 is maintained in a monomeric state through interaction of the NLD with the ER resident chaperone BiP. Upon ER stress, Grp78 binds to unfolded proteins as they accumulate, permitting the released NLD to form homodimers. Dimerization of the NLD in turn leads to the activation of the protein kinase and RNase activities in the cytosolic domain of ERN1."
ENR2 is readily distinguished from its ERN1 paralog at tBlastn by including the two following exons which bring percent identity to 62%: ERN2_monDom KLPFTIPELVHASPCRSSDGVLYTGRKQDTWFMVDPKSGKKQTMLSTETWDGLYPSAPLLYIGRTQYTVTMYDPRSQALRWNTTYRGYSA KLPFTIPELV ASPCRSSDG+LY G+KQD W++VD +G+KQ LS+ + L PS LLY+GRT+YT+TM+D +S+ LRWN TY Y+A ERN1_monDom KLPFTIPELVQASPCRSSDGILYMGKKQDIWYVVDLMTGEKQQTLSSAFAESLCPSTSLLYLGRTEYTITMFDTKSRELRWNATYFDYAA The first alignment shows ERN2 orthologs in vertebrates, the second as difference relative to opossum, the third ERN1 orthologs. The ancestral nature of the CpG hotspot is shown in nucleotides in the final columns. ^ * ^ * ^ * ERN2_homSap KLPFTIPELVHASPCRSSDGVFYT ERN2_homSa .....................F.. ERN1_homSap KLPFTIPELVQASPCRSSDGILYM CG Human ERN2_panTro KLPFTIPELVHASPCRSSDGVFYT ERN2_panTr .....................F.. ERN1_panTro KLPFTIPELVQASPCRSSDGILYM CG Chimp ERN2_ponAbe KLPFTIPELVHASPCRSSDGVFYT ERN2_ponAb .....................F.. ERN1_ponAbe KLPFTIPELVQASPCRSSDGILYM -- Gorilla ERN2_rheMac KLPFTIPELVHASPCRSSDGVFYT ERN2_rheMa .....................F.. ERN1_rheMac KLPFTIPELVQASPCRSSDGILYM CG Orangutan ERN2_calJac KLPFTIPELVHASPCRSSDGVFYT ERN2_calJa .....................F.. ERN1_calJac KLPFTIPELVQASPCRSSDGILYM CG Rhesus ERN2_tarSyr KLPFTIPELVHASPCRSSDGVFYT ERN2_tarSy .....................F.. ERN1_tarSyr KLPFTIPELVQASPCRSSDGILYM CG Marmoset ERN2_micMur KLPFTIPELVHASPCRSSDGVFYT ERN2_micMu .....................F.. ERN1_micMur KLPFTIPELVQASPCRSTDGILYM CG Tarsier ERN2_tupBel KLPFTIPELVHASPCRSSDGVFYT ERN2_tupBe .....................F.. ERN1_otoGar KLPFTIPELVQASPCRSSDGILYM CG Mouse_lemur ERN2_musMus KLPFTIPELVHASPCRSSDGVFYT ERN2_musMu .....................F.. ERN1_tupBel KLPFTIPELVQASPCRSSDGILYM -- Bushbaby ERN2_ratNor KLPFTIPELVHASPCRSSDGVFYT ERN2_ratNo .....................F.. ERN1_musMus KLPFTIPELVQASPCRSSDGILYM CG TreeShrew ERN2_cavPor KLPFTIPELVHTSPCRSSDGVFYT ERN2_cavPo ...........T.........F.. ERN1_ratNor KLPFTIPELVQASPCRSSDGILYM CG Mouse ERN2_speTri KLPFTIPELVHASPCRSSDGVFYT ERN2_speTr .....................F.. ERN1_dipOrd KLPFTIPELVQASPCRSSDGILYM CG Rat ERN2_oryCun KLPFTIPELVHASPCRSSDGVFYT ERN2_oryCu .....................F.. ERN1_cavPor KLPFTIPELVQASPCRSSDGILYM -- Kangaroo_rat ERN2_ochPri KLPFSIPELVHASPCRSSDGVFYT ERN2_ochPr ....S................F.. ERN1_speTri KLPFTIPELVQASPCRSSDGILYM CG Guinea_pig ERN2_turTru RLPFTIPELVHASPCRSSDGVFYT ERN2_turTr R....................F.. ERN1_oryCun KLPFTIPELVQASPCRSSDGILYM CG Squirrel ERN2_bosTau RLPFTIPELVHASPCRSSDGVFYT ERN2_bosTa R....................F.. ERN1_vicPac KLPFTIPELVQASPCRSSDGILYM CG Rabbit ERN2_equCab KLPFTIPELVHASPCRSSDGVFYT ERN2_equCa .....................F.. ERN1_turTru KLPFTIPELVQASPCRSSDGILYM CG Pika ERN2_felCat RLPFTIPELVHASPCRSSDGVFYT ERN2_felCa R....................F.. ERN1_bosTau KLPFTIPELVQASPCRSSDGILYM -- Alpaca ERN2_canFam KLPFTIPELVHASPCRSSDGVFYT ERN2_canFa .....................F.. ERN1_equCab KLPFTIPELVQASPCRSSDGILYM CG Dolphin ERN2_myoLuc KLPFTIPELVHASPCRSSDGVFYT ERN2_myoLu .....................F.. ERN1_canFam KLPFTIPELVQASPCRSSDGILYM CG Cow ERN2_eriEur KLPFTVPELVHTSPCRSSDGVFYT ERN2_eriEu .....V.....T.........F.. ERN1_myoLuc KLPFTIPELVQASPCRSSDGILYM CG Horse ERN2_sorAra KLPFTIPELVHASPCRSSDGVFYT ERN2_sorAr .....................F.. ERN1_pteVam KLPFTIPELVQASPCRSSDGILYM CG Cat ERN2_loxAfr KLPFTIPELVHASPCRSSDGVFYT ERN2_loxAf .....................F.. ERN1_eriEur KLPFTIPELVQASPCRSSDGILYM CG Dog ERN2_echTel KLPFTIPELVLASPCRSSDGVFYT ERN2_echTe ..........L..........F.. ERN1_sorAra KLPFTIPELVQASPCRSSDGILYM CG Microbat ERN2_dasNov KLPFTIPELVHTSPCRSSDGIFYT ERN2_dasNo ...........T........IF.. ERN1_loxAfr KLPFTIPELVQASPCRSSDGILYM -- Megabat ERN2_monDom KLPFTIPELVHASPCRSSDGVLYT ERN2_monDo KLPFTIPELVHASPCRSSDGVLYT ERN1_proCap KLPFTIPELVQASPCRSSDGILYM CG Hedgehog ERN2_macEug KLPFTIPELVHASPCRSSDGVFYT ERN2_macEu .....................F.. ERN1_echTel KLPFTIPELVQASPCRSSDGILYM CG Shrew ERN2_sarHar1 KLPFTIPELVQASPCRSSDGIFYM ERN2_sarHa ..........Q.........IF.M ERN1_dasNov KLPFTIPELVQASPCRSSDGILYM -- Elephant ERN2_sarHar2 KLPFTIPELVQASPCHSSDGIFYM ERN2_sarHa ..........Q....H....IF.M ERN1_choHof KLPFTIPELVQASPCRSSDGILYM -- Rock_hyrax ERN2_ornAna KLPFTIPELVQSSPCRSSDGILYT ERN2_ornAn ..........QS........I... ERN1_monDom KLPFTIPELVQASPCRSSDGILYM CG Tenrec ERN2_anoCar KLPFTIPELVQSSPCRSSDGIIYT ERN2_anoCa ..........QS........II.. ERN1_ornAna KLPFTIPELVHASPCRSSDGILYM CG Armadillo ERN2_taeGut KLPFTIPELVQSSPCRSSDGVLYT ERN2_taeGu ..........QS............ ERN1_galGal KLPFTIPELVQASPCRSSDGILYM CG Opossum ERN2_galGal KLPFTIPELVQASPCRSSDGILYM ERN2_galGa ..........Q.........I..M ERN1_taeGut KLPFTIPELVQASPCRSSDGILYM CG Platypus ERN2_xenTro KLPFTIPELVQSSPCRSSDGILYT ERN2_xenTr ..........QS........I... ERN1_anoCar KLPFTIPELVQASPCRSSDGILYM CG Lizard ERN2_xenLae KLPFTIPELVQSSPCRSSDGILYT ERN2_xenLa ..........QS........I... ERN1_xenTro KLPFTIPELVQSSPCRSSDGILYT CG Tetraodon ERN2_tetNig KLPFTIPELVQASPCRSSDGVLYM ERN2_tetNi ..........Q............M ERN1_tetNig KLPFTIPELVQASPCRSSDGVLYM CG Fugu ERN2_takRub KLPFTIPELVQASPCRSSDGVLYM ERN2_takRu ..........Q............M ERN1_takRub KLPFTIPELVQASPCRSSDGVLYM CT Stickleback ERN2_gasAcu KLPFTIPDLVQSAPCRSSDGILYT ERN2_gasAc .......D..QSA.......I... ERN1_gasAcu KLPFTIPELVQASPCRSSDGVLYM CT Medaka ERN2_oryLat KLPFTIPELVQSAPCRSSDGILYT ERN2_oryLa ..........QSA.......I... ERN1_oryLat KLPFTIPELVQASPCRSSDGVLYM CG Lamprey ERN2_calMil KLPFTIPELVQSSPCRSSDGILYT ERN2_calMi ..........QS........I... ERN1_danRer KLPFTIPELVQASPCRSSDGILYM ERN2_petMar KLPFTIPELVHASPCRTSDGVLYT ERN2_petMa ................T....... ERN_braFlo KLPFTIPELVNASPCKSSDGILYT ERN_braFlo ..........N....K....I...
Case of MGAT5
chr4_4859 MGAT5 12 >contig00001 length=538 numreads=5 21 C=2(61) Y=2(56) LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE ................................................. ^ Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5 and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in two tasmanian devil (here one is identical and the other differs from Monodelphis by C->Y) and finally the number of experimental reads that confirm the nucleotide difference and the sum of the quality scores. The sequences were assembled by Newbler (the official 454 assembler).
Pseudogene issues: No processed pseudogenes relevent to this exon are seen by Blat of human and opossum sequence. Some questionable sequence occurs in tarsier and sloth but may be due to low coverage read or assembly error. These fragmentary sequences also have cysteine at the position in question.
Paralog issues: This gene has a moderately similar paralog, MGAT5B, with a similar enzymatic role (beta1,6-N-acetylglucosaminyltransferase). The opossum MGAT5B protein differs at 12 positions out of 49 from opossum MGAT5, whereas human and marsupial MGAT5A differ at one residue. Consequently the two paralogs are readily distinguished within vertebrates. This is moot because 33 of 33 available MGAT5B also have cysteine at the position in question (data not shown).
Homoplasy (recurrent mutation) issues: The alignments below show tyrosine has never replaced cysteine in any other species. This cysteine is extremely invariant in both paralogs, tracing back to lophotrochozoa and cnidaria.
Known variations: No human disease alleles have been mapped to either paralog. None of 9 SNP tracks at the UCSC browser show human variation in this exon.
Side issues: The column marked with an asterisk in the difference alignment below indicates a non-conservative phyloSNP K-->I that occured in the theran mammal stem after platypus divergence. All three marsupial sequences including tasmanian devil have isoleucine in this position as do all 30 of the available placental mammal sequences, suggesting that both the lysine and the isoleucine continue to be under strong selection. No comparable shift occured in the theran stem for MGAT5B where the residue is arginine in all species, a basic residue similar to lysine.
Structural significance: The MGAT5 gene supposedly encodes a conventional enzyme, mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Yet surprisingly, no determined 3D structure exists at PDB relevent to the configuration of this exon -- nor indeed the large 741 residue protein. This is very peculiar because glycosyl transerfases are a well-studied group of enzymes (nearly 100 loci in human) and might be expected to bind UDP-GlcNAc (like MGAT4A or MGAT3).
Only a small region of the protein have a prediction at ModBase using 2f9fA, a remote mannosyltransferasee from Archaeoglobus fulgidus. Luckily the model covers the cysteine at issue, showing two helices and a beta sheet.
SwissProt does not annotate the cysteine at position 532 as part of a disulfide or active site; the predicted location (Golgi) can have homodimer disulfides of similar enzymes, though this is a complex topic. Although all 20 cysteines in this protein are conserved human to opossum, this could be a consequence of the overall sequence identity of 90%. Twelve of the cysteines, not including the Sarcophilus variant, are found in the last 140 residues, perhaps forming a disulfide knot. All but 1 of these cysteines is conserved in the pre-Bilateran anemone Nematostella (which enriches relative to overall percent identity of 43%).
Highest MGAT5 expression occurs in brain, heart, kidney, and placenta. No domains other than a signal peptide and 6 of its own glycosylation target sites are found by online tools such as SMART.
Although the bulky tyrosine substitution is conservative in the sense of polar nature and perhaps hydrogen-bonding capacity, it cannot replace these specialized functions of cysteine. Considering the extreme conservation of this cysteine, this substitution must have a substantial-- perhaps even disabling -- impact on enzymatic function.
Functional significance: In view of the facial tumor situation in tasmanian devils, OMIM's account of prior research in mouse on this gene is quite interesting. Less is known about MGAT5B though it also functions in the synthesis of complex cell surface N-glycans.
" Malignant transformation is accompanied by increased beta-1,6-GlcNAc branching of N-glycans attached to Asn-X-Ser/Thr sequences in mature glycoproteins... The amount of MGAT5 products correlates with disease progression... Mgat5-deficient mice, which are born healthy but develop various abnormalities as adults...Mgat5-deficient mice showed kidney autoimmune disease, enhanced delayed-type hypersensitivity, and increased susceptibility to experimental autoimmune encephalomyelitis...The Golgi enzyme beta1,6 N-acetylglucosaminyltransferase V (Mgat5) is up-regulated in carcinomas and promotes the substitution of N-glycan with poly N-acetyllactosamine, the preferred ligand for galectin-3 (Gal-3)...inhibitors of MGAT5 might be useful in the treatment of malignancies by targeting their dependency on focal adhesion signaling for growth and metastasis."
^ ^ * MGAT5_homSap LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE homSap MGAT5_panTro LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. panTro MGAT5_gorGor LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. gorGor MGAT5_ponAbe LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. ponAbe MGAT5_rheMac LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. rheMac MGAT5_calJac LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. calJac MGAT5_micMur LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE .............................S................... micMur MGAT5_otoGar LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. otoGar MGAT5_tupBel LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. tupBel MGAT5_musMus LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. musMus MGAT5_ratNor LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. ratNor MGAT5_criGri LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE .............................S................... criGri MGAT5_dipOrd LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. dipOrd MGAT5_cavPor LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. cavPor MGAT5_speTri LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. speTri MGAT5_oryCun LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. oryCun MGAT5_ochPri LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. ochPri MGAT5_vicPac LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. vicPac MGAT5_susScr LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. susScr MGAT5_turTru LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. turTru MGAT5_bosTau LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. bosTau MGAT5_equCab LFAGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ..A.............................................. equCab MGAT5_felCat lfvgLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE .............................S................... felCat MGAT5_canFam LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE .............................S................... canFam MGAT5_myoLuc LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. myoLuc MGAT5_eriEur LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. eriEur MGAT5_sorAra LFVGLGFPYEGPAPLEAIANGCAFLNPKFSPPKSSKNTDFFIGKPTLRE .............................S................... sorAra MGAT5_loxAfr LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. loxAfr MGAT5_proCap LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. proCap MGAT5_echTel LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRE ................................................. echTel MGAT5_monDom LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE ..........................V...................... monDom MGAT5_macEug LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE ..........................V...................... macEug MGAT5_sarHar1 LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE ..........................V...................... sarHar1 MGAT5_sarHar2 LFVGLGFPYEGPAPLEAIANGYAFLNVKFNPPKSSKNTDFFIGKPTLRE .....................Y....V...................... sarHar2 MGAT5_ornAna LFVGLGFPYEGPAPLEAIANGCAFLNLKFNPPKSSKNTDFFKGKPTLRE ..........................L..............K....... ornAna MGAT5_galGal LFVGLGFPYEGPAPLEAIANGCAFLNLRFNPPKSSKNTEFFKGKPTLRE ..........................LR..........E..K....... galGal MGAT5_taeGut LFVGLGFPYEGPAPLEAIANGCAFLNLRFNPPKSSKNTDFFKGKPTLRE ..........................LR.............K....... taeGut MGAT5_anoCar LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFKGKPTLRE .........................................K....... anoCar MGAT5_xenTro LFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSRNTDFFKGKPTLRE ...................................R.....K....... xenTro MGAT5_tetNig VFVGLSFPYEGPAPLEALANGCIFLNPRLKPPQSSLNSEFFKEKPNIRE V....S...........L....I....RLK..Q..L.SE..KE..NI.. tetNig MGAT5_takRub LFVGLSFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFKGKPTLRE .....S...................................K....... takRub MGAT5_gasAcu LFVGLSFPYEGPAPLEAIANGCAFLNPKFSPAKSSKNTDFFKGKPTLRE .....S.......................S.A.........K....... gasAcu MGAT5_oryLat LFVGLSFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFKGKPTLRE .....S...................................K....... oryLat MGAT5_danRer LFVGLSFPYEGPAPLEAIANGCAFLNPRFDPAKSSKNTDFFKGKPTLRE .....S.....................R.D.A.........K....... danRer MGAT5_oncMyk LFVGLSFPYEGPAPLEAIANGCAFLNPKFTPPKSSKNTDFFKGKPTLRE .....S.......................T...........K....... oncMyk MGAT5_pimPro LFVGLSFPYEGPAPLEAIANGCAFLNPRFDPSKSSKNTDFFKGKPTLRE .....S.....................R.D.S.........K....... pimPro MGAT5_calMil LFVGLGFPYEGPAPLEAIANGCAFLNPRFNPPKSSKNTEFFKGKPTLRE ...........................R..........E..K....... calMil MGAT5_petMar LFVGLGFPYEGPAPLEAIANGCVFLNPRFRPPKSSKNTDFFKGKPTLRE ......................V....R.R...........K....... petMar MGAT5_braFlo LFVGLGFPYEGPAPLEAIASGCVFLNPKFTQPKSRLNTKFFEGKPTFRE ...................S..V......TQ...RL..K..E....F.. braFlo MGAT5_strPur LFIGLGFPYEGPAPLEAVANGCVFLNPKFNPPKNYQNTKFFQGKPTSR. MGAT5_helRob LFIGLGFPYEGPAPLEAIAAGCVFINPKFNPPHSSLNTKFFKGKPTARE MGAT5_nemVec VFIGLGFPYEGPAPLEAIQSGCVFLNAKFDPPHDRVNTPFFKNKPTLRK
Note: the species with unfamiliar genSpp acronyms are Cricetulus griseus, Oncorhynchus mykiss, Pimephales promelas , Callorhinchus milii, Branchiostoma floridae, Strongylocentrotus purpuratus, Helobdella robusta, Nematostella vectensis, and Acropora millepora.
Here the opossum protein is broken into its 16 coding exons with phases (base overhangs at split codons) shown: >MGAT5_monDom length=743 0 MAFFAPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQHESSSMLREQILDLSKRYIKALAEENRNVVDGPYVGVMTAY 1 2 DLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTTTTAAPSSIAAFEKISVA 1 2 DIINGAQEKCELPPMDGFPHCEGKIK 0 0 WMKDMWRTDPCYANYGVDGSTCSFFIYLSE 0 0 VENWCPHLPWRAKNPYEEPDQNSM 0 0 AEIRTDFNLLYGMMKRHEEFRWMILRIRRMADAWIEAIKSLAEKQNLEKRKRKK 0 0 ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE 2 1 IMKRVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQ 2 1 CMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMF 1 2 PHTPDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDSFWK 0 0 NKKEYLDIIHTYMEVHATVYGSSTNHMPSYVKNHGILSGRDLQFLLRETK 0 0 LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE 0 0 LTSQHPYAEVFIGRPHVWTVNPTDHREVENAVKAILNQK 0 0 IEPYMPYEFTCEGMLQRMNAFIEKQ 0 0 DFCHGQVMWPPLNALQVKLSEPGKSCKQVCQENQLICEPSFFQHLNKDKDVLK 2 1 YEVICHTTELANDILVPSYDDKKKHCVFQGDLLLFSCAGAHPKHKRICPCRDYIKGQVALCQDCL* 0 >MGAT5_sacHar Sarcophilus harrisii (tasmanian_devil) one match to exon 1: FPUIIJ301C96S1 0 MAFFAPWKLSSQN*GFSWLTFGFIWGMMLLHFTIQQRTQHESSSMLREQILDLSKRYIKALAEENRNVVDGPYVGVMTAY 1 2 DLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTTTTAVPSSIAAFEKIsVA 1 2 DIINGAQEKCELPPMDGFPHCEGKIK 0 0 0 0 0 0 AEIRTDFHLLYGMMKRHEEFRWMILRIRRMADAWIEAIKSLAEKQNLEKRKRKK 0 0 ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE 2 1 IMKRVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQ 2 1 CMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMF 1 2 AHTPDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDNFWK 0 0 NKKEYLDIIHTYMEVHATVYGSSTNHMPSYVKNHGILSGRDLQFLLRETK 0 0 LFVGLGFPYEGPAPLEAIANGYAFLNVKFNPPKSSKNTDFFIGKPTLRE 0 0 0 0 IEPYMPYEFTCEGMLQRMNAFIEKQ 0 0 2 1 YEVVCHTTELANDILVPSYDDRKKHCVFQGDLLLFSCAGAHPKHKRICPCRDYIKGQVALCQDCL* 0 The premature stop codon in the first exon is likely read error (1 bp dropped, 1 bp later added): atggctttctttgctccatggaaattatcctctcagaaactagggtttttcctggtgact M A F F A P W K L S S Q K L G F F L V T correct monDom frame W L S L L H G N Y P L R N - G F S W - L 6 residue observed frameshifts in sarHar N*GFSWL G F L C S M E I I L S E T R V F P G D F irrelevent 3rd reading frame MGAT5 has 16 exons. The key one here is 12. Alignment of MGAT5_sarHar to opossum shows only 5 differences in 589 residues available for comparison. Alignment of Monodelphis to human establishes that MGAT5 is better conserved than the average gene: Identities = 673/744 (90%), Positives = 708/744 (95%), Gaps = 2/744 (0%) monDo 1 MAFFAPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQHESSSMLREQILDLSKRYIKA 60 MA F PWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQ ESSSMLREQILDLSKRYIKA homSa 146 MALFTPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSSMLREQILDLSKRYIKA 325 monDo 61 LAEENRNVVDGPYVGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTT 120 LAEENRNVVDGPY GVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLV+ G + +T homSa 326 LAEENRNVVDGPYAGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVV--NGTGTNSTN 499 monDo 121 TTAAPSSIAAFEKISVADIINGAQEKCELPPMDGFPHCEGKIKWMKDMWRTDPCYANYGV 180 +T A S+ A EKI+VADIINGAQEKC LPPMDG+PHCEGKIKWMKDMWR+DPCYA+YGV homSa 500 STTAVPSLVALEKINVADIINGAQEKCVLPPMDGYPHCEGKIKWMKDMWRSDPCYADYGV 679 monDo 181 DGSTCSFFIYLSEVENWCPHLPWRAKNPYEEPDQNSMAEIRTDFNLLYGMMKRHEEFRWM 240 DGSTCSFFIYLSEVENWCPHLPWRAKNPYEE D NS+AEIRTDFN+LY MMK+HEEFRWM homSa 680 DGSTCSFFIYLSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWM 859 monDo 241 ILRIRRMADAWIEAIKSLAEKQNLEKRKRKKILVHLGLLTKESGFKIAENAFSGGPLGEL 300 LRIRRMADAWI+AIKSLAEKQNLEKRKRKK+LVHLGLLTKESGFKIAE AFSGGPLGEL homSa 860 RLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGEL 1039 monDo 301 VQWSDLITSLYLLGHDIRISASLAELKEIMKRVVGNRSGCPTVGDRIVELIYIDIVGLAQ 360 VQWSDLITSLYLLGHDIRISASLAELKEIMK+VVGNRSGCPTVGDRIVELIYIDIVGLAQ homSa 1040 VQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQ 1219 monDo 361 FKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHT 420 FKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHT homSa 1220 FKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHT 1399 monDo 421 PDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDSFWKNKKEYLDIIHTYMEVHAT 480 PDNSFLGFVVEQHLNSSDI HINEIKRQNQSLVYGKVDSFWKNKK YLDIIHTYMEVHAT homSa 1400 PDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHAT 1579 monDo 481 VYGSSTNHMPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNVK 540 VYGSST ++PSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLN K homSa 1580 VYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPK 1759 monDo 541 FNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVNPTDHREVENAVKAILNQK 600 FNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTV+ + EVE+AVKAILNQK homSa 1760 FNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQK 1939 monDo 601 IEPYMPYEFTCEGMLQRMNAFIEKQDFCHGQVMWPPLNALQVKLSEPGKSCKQVCQENQL 660 IEPYMPYEFTCEGMLQR+NAFIEKQDFCHGQVMWPPL+ALQVKL+EPG+SCKQVCQE+QL homSa 1940 IEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQL 2119 monDo 661 ICEPSFFQHLNKDKDVLKYEVICHTTELANDILVPSYDDKKKHCVFQGDLLLFSCAGAHP 720 ICEPSFFQHLNKDKD+LKY+V C ++ELA DILVPS+D K KHCVFQGDLLLFSCAGAHP homSa 2120 ICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHP 2299 monDo 721 KHKRICPCRDYIKGQVALCQDCL* 744 +H+R+CPCRD+IKGQVALC+DCL homSa 2300 RHQRVCPCRDFIKGQVALCKDCL* 2371
Full length genes appear available from GenBank and genome projects for mouse, rat (NM_001107068), dog (wgs exons), horse (XM_001489091), wallaby (wgs exons), and platypus (XM_001520380). Because this gene is 90% conserved at marsupial, placental mammals will not be informative -- indeed it is necessary to go to greater phylogenetic depth than lamprey to define the ultra-conserved residues in this protein:
>MGAT5_macEug nearly identical to monDom; 3 exons are missing, 2 partial exons, exon 4 has frameshifts 0 MAFFAPWKLSSQKLGFFL 1 2 DLKKTLAVLLDNILQRIGKLESKVDNLVINGTGANSTNTTTTAVPSSIAAFEKISVA 1 2 DIINGAQEKCELPPMDGFPHCEGKIK 0 0 WMKDiWRTDPCYANYGVDGSTCSFFIYLSE 0 0 VENWCPHLPWRAKNPYEEPDQNSM 0 0 0 0 ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE 2 1 2 1 GTEPEFNHANYAQSKGHKTP 1 2 aHTPDNSFLGFVVEQHLNSSDIKHINEIKRQNQSLVYGKVDSFWK 0 0 NKKEYLDIIHTYMEVHATVYGSSTNHMPSYVKNHGILSGRDLQFLLRETK 0 0 LFVGLGFPYEGPAPLEAIANGCAFLNVKFNPPKSSKNTDFFIGKPTLRE 0 0 LTSQHPYAEVFIGRPHVWTVNPTDHREVENAVKAILNQK 0 0 IEPYMPYEFTCEGMLQRMNAFIEKQ 0 0 DFCHGQVMWPPLNALQVKLSEPGKSCKQVCQENQLICEPSFFQHLNKDKDVLK 2 1 YEVICHTTELANDILVPSYDDKKKHCVFQGDLLLFSCAGAHPKHKRICPCRDYIKGQVALCQDCL* 0 >MGAT5_galGal 87% identical to opossum MAFPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQQTQHESSSVLREQILDLSKRYIKALAEENKNVVDGPYVGTVTAY DLKKTLAVLLDNILQRIGKLESKVENLVLNGTGANSTNTTTPAPSLGAVEKLNVA DLINGAQEQCELPPMDGFPHCEGKIK WMKDMWRSDPCYASYGVDGSTCSFFIYLSE VENWCPRLPWRAKNPNEETDQKTV AEIRINFDPLYKMMSRHEEFRWMTLRIRRMADTWIEAIKSLAEKQNLENRKRKK ILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKE IMKKVVGNRSGCPTQGDKVVELIYIDIVGLTQFKKTLGPSWVHYQ CMLRVLDSFGTEPEFNHAHYAQSKGHKTPWGKWNLNPQQFYTMF PHTPDNSFLGFVVEQHLNSSDIKHINDIKRQNQSLVYGKVDNFWK DKKAYLDVIHTYMEVHGTVHGTSTIYIPGYVKNHGILSGRDLQFLLRETK LFVGLGFPYEGPAPLEAIANGCAFLNLRFNPPKSSKNTEFFKGKPTLRE LTSQHPYAEVYIGKPHVWTVDINNLSEVEKAVKSILNQK IDPYLPYEFTCEGMLQRMNAFIERQ DFCHGQVMWPPLSALQVKLAEPGKSCKQVCQESQLICEPSFFQHLNKDKALLK HNIECLTTESANDILVPSFDGRRKHCVFQGDLLLFSCAGSHPTHRRICPCRDYIKGQVALCKDCL* >MGAT5_nemVec Nematostella vectensis (sea anemone) XM_001641404 43% identical to opossum 19 of 20 cysteines conserved MIATKGRPTFKLSAHRIGIVFIIISFIWGLYLIKIQLDERNSQPDYLKGRIIHLSKEYIRALAREKGVYGIDGQPSTQQGVGDLKKATAVLLQSMLERIHVL EKQVEGVIVNSTLEFEILASQIKSLNTTFSLHLSNHSYVSANSCVIPDDPSYPECRQKVMWMRNFWKTHECYAKDHGVNGTICSFLVYLSEVENWCPKFPGRMKPTSRATTEGADL HRSDVQGLLGLLNDQDPIKFKWIKNRINQMWPQWLSALEDLKKKRDLKKIKQKKILVHIGLLANERALHFAANADKGGPLGELVQWSDLIASLYLLGHDVTVTADIPRLQGIFGKL RGPAKKPCPTTIKNDYDLIYLDYYGVKQMQTKVGQFTQSFKCKFRIVDSFGTEAQFNYAGFTEKVPGGSMALWGRHNLNLKQFMTMFPHSPDNSFLGFVVGEEPTPDPHPKKKKAR ALVYGKHYYMWKDLKQRSFLDVINKYMEIHATVGGGIKKWVPSYVINHGVLPSLEVQKLLQDSMIFVGLGFPYEGPAPLEAIAHGCFFLNTKYHPPRNRINTPFFKDKPTLRQITS QHPYAEDYIGQPYVYTVDINDLNKIEAVMKEIMMAEPVSPYLPYEFTHKGMLERLHVFIENQNFCGQNLWPPLNALQARKGAMGSSCKETCHSLGLVCEPQYFPAINTKERMTRSG FPCNTTRVEDMPSLVAPGYRDDPPVCLRQAQNLLFSCTANSPTTKRLCPCRDFKKGQVALCSKC*
Case of ACTL6B
chr2_18546 ACTL6B 11 >contig00001 length=502 numreads=11 GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ^ 3 G=4(94) R=7(213) Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5 and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in tasmanian devil (in this case, one individual differs from Monodelphis by G->R), then differences between the two devils, and finally the number of experimental reads that confirm the nucleotide difference and the sum of the quality scores. The sequences were assembled by Newbler.
The change from small non-polar glycine to bulky positively charged lysine is highly non-conservative, especially at a highly conserved residue such as this. Again the change in Sarcophilus is at a CpG hotspot, this time with a mildly unusual transversion of the C to the purine G.
The well-studied protein here is a member of a family of actin-related proteins (ARPs) which have significant homology to conventional actins, in particular sharing the actin fold (an ATP-binding cleft) as common feature. ACTL6B and its 83% identical paralog ACTL6A are involved in diverse cellular processes such as vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. Both have 14 coding exons. The entire exon containing the G-->R is highly conserved including the glycine.
Pseudogene issues: Blat of full length sequence to human shows no recent processed or segmental pseudogenes. However more sensitive methods show a half dozen processed pseudogenes on different chromosomes plus one for ACTL6A. And opossum assembly, which has all 14 exons, also contains a fairly recent processed pseudogene with 91.5% identity. This locus has internal stop codons and ELSD in place of GLSG for the key glycine. This pseudogene arose from ACTL6A, not ACTL6B.
Retroposed Genes, Including Pseudogenes (retroMrnaInfo UCSC track): ACTL6B at chrX:53188763-53189824 ACTL6B at chr9:110656744-110657692 ACTL6A at chr14:49217726-49219292 ACTL6B at chr7:5533936-5535808 ACTL6B at chr6:46280879-46281761 ACTL6B at chr17:77092347-77093972 ACTL6B at chr1:227633849-227635482
Sarcophilus also has one or more processed pseudogenes which considerably complicates the interpretation of tblastn output. However reads FP1I63R01ARR6N etc show two consecutive exons, the first of which is the G-->R version of the exon and the second identical to the following exon from opossum. The spacing between the two exons is 132 bp, more than adequate for a mammalian intron (whose lower limit is about 78 bp). Other reads span two exon for the normal version of the exon such as FKUJDAX01DZSZO etc again with same intron spacing. (Processed pseudogenes may later acquire pseudo-introns in the form of retroposons so RepeatMaskers needs to be run on the intervening sequence.)
>FP1JAYN01EIJD3 length=493 xy=1734_1049 region=1 run=R_2009_01_29_12_22_00_ monDo: 37 VKGLSGNTMLGVGHVVTTSIGMCDIDIRP 65 ++GLS NTMLGVGHVVTTSIGMCDIDIRP sacHa: 386 LQGLSRNTMLGVGHVVTTSIGMCDIDIRP 300 monDo: 66 GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP 97 GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP sacHa: 168 GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP 73 Newbler has a bad tendency to create faux frameshifts: Query: 82 ggtctctacggcagtgtcattgtcactggagggaacacactcttgcaaggctttactgac 141 |||||||||||||||||||||| |||||||||||||||||| |||||||| ||||||||| Sbjct: 167 ggtctctacggcagtgtcattg-cactggagggaacacactgttgcaaggttttactgac 109 FP1I63R01APY7E Query: 82 ggtctctacggcagtgt-cattgtcactggagggaacacactcttgcaaggctttactga 140 ||||||||||||||||| |||||||||||||||||||||||| |||||||| |||||||| Sbjct: 268 ggtctctacggcagtgttcattgtcactggagggaacacactgttgcaaggttttactga 327 FKUJDAX01AWWZ3 Query: 82 ggtctctacggcagtgtcattgtcactggagggaac-acactcttgcaagg 131 |||||||||||||||||||||||||||||||||||| ||||| |||||||| Sbjct: 268 ggtctctacggcagtgtcattgtcactggagggaacgacactgttgcaagg 318 FKUJDAX01DZSZO
Paralog issues: There is potential for confusion with the paralog ACTL6A. This wouldn't normally matter because all species in this gene too have glycine at the arginine-substituted site. However its pseudogene could present problems because its decay may have taken a different path in Sarcophilus than in Monodelphis giving the R (instead of D), assuming the pseudogene was formed prior to divergence of these species. Indeed, Macropus eugenii appears to have two processed pseudogenes; one of this has R in place of a glycine 4 residues earlier. It will prove necessary to consider adjacent regions in Sarcophilus reads to determine whether the feature is a pseudogene.
To summarize, this appears to be a valid coding SNP but the situation with paralogs, pseudogenes, and errors intrinsic to the 454 platform makes it unfavorable for rapid screening. It would be necessary to require matches of flanking intronic regions on both sides to be sure that the right locus is being investigated.
Comparison of gene to pseudogene in opossum: 000000889 E R L R I P E G L F D P S N V K G L S G 000000948 <<<<<<<<< | X | K | | | | | | | | | | | | E | | D <<<<<<<<< 250390825 gagtgactcaagattcctgaagggttatttgacccatctaatgtgaaggaattgtcagac 250390766 000000949 N T M L G V G H V V T T S I G M C D I D 000001008 <<<<<<<<< | | | | | | S | | | | | | F | | | | | | <<<<<<<<< 250390765 aacacaatgttgggagtcagtcatgttgttaccacaagctttgggatgtgtgacattgac 250390706 000001009 I R P G L Y G S V I V T G G N T L 000001059 <<<<<<<<< F | | | | | D N M L G A | | | I | <<<<<<<<< 250390705 tttagaccgggactttatgacaatatgttaggggcgggaggaaacattctg 250390655 Comparison of ACTL6A_homSap gene to pseudogenes in wallaby: macEu: 1063 FPVGYNCNFGVEQLKITERLFDPSNVKRLSGNPMLGVSHVVTTRIGMCDIDIRPGLYGTV 1242 FP GYNC+FG E+LKI E LFDPSNVK LSGN MLGVSHVVTT +GMCDIDIRPGLYG+V homSa: 289 FPNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDIRPGLYGSV 348 macEu: 48 PNVYKCGFGAEHFKIPEGLFDRSNMKGLSGNTMLGISHVVTKSTGMCDIDIRPGFYISVI 227 PN Y C FGAE KIPEGLFD SN+KGLSGNTMLG+SHVVT S GMCDIDIRPG Y SVI homSa: 290 PNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDIRPGLYGSVI 349
Homoplasy (recurrent mutation) issues:
Known variations:
Side issues:
Structural significance:
Functional significance:
* * * ACTL6B_homSap GLSGNTMLGVGHVVTTSIGMCDIDIRP GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_homSap GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_panTro GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_panTro GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_gorGor GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_gorGor GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_ponAbe GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_ponAbe GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_rheMac GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_rheMac GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_calJac GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_calJac GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_tarSyr GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_tarSyr GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_micMur GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_micMur GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_otoGar GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_otoGar GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_tupBel GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_tupBel GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_musMus GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_musMus GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_ratNor GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_ratNor GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_dipOrd GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_dipOrd GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_cavPor GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_cavPor GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_ochPri GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_ochPri GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_turTru GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_turTru GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_bosTau GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_bosTau GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_equCab GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_equCab GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_felCat GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_felCat GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_canFam GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_canFam GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_myoLuc GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_myoLuc GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_pteVam GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_pteVam GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_eriEur GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_eriEur GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_loxAfr GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_loxAfr GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_proCap GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_proCap GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_echTel GLSGNTMLGVGHVVTTSIGMCDNDIRP ......................N.... ACTL6B_echTel GLSGNTMLGVGHVVTTSIGMCDNDIRP ACTL6B_monDom GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_monDom GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_ornAna GLSGNTMLGVSHVVTTSVGMCDIDIRP ..........S......V......... ACTL6B_ornAna GLSGNTMLGVSHVVTTSVGMCDIDIRP ACTL6B_galGal GLSGNTMLGVSHVVTTSVGMCDIDIRP ..........S......V......... ACTL6B_galGal GLSGNTMLGVSHVVTTSVGMCDIDIRP ACTL6B_taeGut GLSGNTMLGVSHVVTTSVGMCDIDIRP ..........S......V......... ACTL6B_taeGut GLSGNTMLGVSHVVTTSVGMCDIDIRP ACTL6B_anoCar GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_anoCar GLSGNTMLGVGHVVTTSIGMCDIDIRP ACTL6B_xenTro GLSGNTMLGVSHVVTTSVGMCDIDIRP ..........S......V......... ACTL6B_xenTro GLSGNTMLGVSHVVTTSVGMCDIDIRP ACTL6B_tetNig GLSGNTMLGVSHVVTTSVGMCDIDIRP ..........S......V......... ACTL6B_tetNig GLSGNTMLGVSHVVTTSVGMCDIDIRP ACTL6B_takRub GLSGNTMLGVSHVVTTSVGMCDIDIRP ..........S......V......... ACTL6B_takRub GLSGNTMLGVSHVVTTSVGMCDIDIRP ACTL6B_gasAcu GLSGNTMLGVGHVVTTSVGMCDIDIRP .................V......... ACTL6B_gasAcu GLSGNTMLGVGHVVTTSVGMCDIDIRP ACTL6B_oryLat GLSGNTMLGVGHVVTTSVGMCDIDIRP .................V......... ACTL6B_oryLat GLSGNTMLGVGHVVTTSVGMCDIDIRP ACTL6B_danRer GLSGNTMLGVGHVVTTSIGMCDIDIRP ........................... ACTL6B_danRer GLSGNTMLGVGHVVTTSIGMCDIDIRP * * * Consensus gLsGnTMlgvgHVVTts!g$CDi.Ir. gLsGnTMlgvgHVVTts!g$CDi.Ir. >ACTL6B_homSap MSGGVYGG DEVGALVFDIGSFSVRAGYAGEDCPK ADFPTTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHVPRDGAEVMSPLKNGM IEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAP WNTRAKREKLTELMFEQYNIPAFFLCKTAVLTA FANGRSTGLVLDSGATHTTAIPVHDGYVLQQ GIVKSPLAGDFISMQCRELFQEMAIDIIPPYMIAAK EPVREGAPPNWKKKEKLPQVSKSWHNYMCN EVIQDFQASVLQVSDSPYDEQ VAAQMPTVHYEMPNGYNTDYGAERLRIPEGLFDPSNVK GLSGNTMLGVGHVVTTSIGMCDIDIRP GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP SMRLKLIASNSTMERKFSPWIGGSILASL GTFQQMWISKQEYEEGGKQCVERKCP* >ACTL6B_monDom MSGGVYGG DEVGALVFDIGSFSVRAGYAGEDCPK ADFPTTVGLLTLEEGGGLELDGEKEKKGKTFHIDTNALHVPRDGAEVMSPLKNGM IEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAP WNTRAKREKLTELMFEQYNIPAFFLCKTAVLTA FANGRSTGLVLDSGATHTTAIPVHDGYVLQQ GIVKSPLAGDFISMQCRELFQEMAIDIIPPYMIAAK EPVREGAPPNWKKKEKLPQVSKSWHNYMCN EVIQDFQASVLQVSDSPYDEQ VAAQMPTVHYEMPNGYNTDYGAERLRIPEGLFDPSNVK GLSGNTMLGVGHVVTTSIGMCDIDIRP GLYGSVIVTGGNTLLQGFTDRLNRELSQKTPP SMRLKLIASNSTMERKFSPWIGGSILASL GTFQQMWISKQEYEEGGKQCVERKCP*
Case of IPO7
chr5_9037 IPO7 23 >contig00001 length=680 numreads=8 SSQVEKHSCSLTEELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ ....*N.....................................................F..................... ^ 59 F=2(72) S=3(53) Read data format: the top row gives project gene name, HGNC gene name and exon number from ENSEMBL monDom5 and human orthology predictions, then Monodelphis amino-acid segment, then sequence differences in tasmanian devil (in this case, both individuals differ from Monodelphis by -> ), then differences between the two devils (here one individual has S at position 59, the other has F), and finally the number of experimental reads that confirm the nucleotide difference and the sum of the quality scores.
Here the Ensembl-predicted sequence for opossum IPO7 is wrong. The exon begins with EELGSD... and the preceding residues are rubbish. The stop codon and N are thus extraneous.
Pseudogene issues: Human has 4 processed pseudogenes originating at various dates. However opossum lacks any detectable by Blat.
Retroposed Genes, Including Pseudogenes (from pseudoGeneLink and retroMrnaInfo UCSC tracks) IPO7 at chr1:209097616-209101414 IPO7 at chr13:23593176-23594670 IPO7 at chr20:25520871-25521227 IPO7 at chrX:51680122-51682234
Paralog issues: IPO8 is somewhat similar but not sufficiently in this exon to engender confusion.
monDom7 EEIPSDEEDTNEARQALHE---RGGGEDEEEDDDDWDEEVLEETALEGFSTPLDLDDG-VDEYQFFT---QALLSRS EE+ SDE+D +E Q E + GED DD++W+E+ EETALEG+ST +D ++ VDEYQ F QA+ SR+ monDom8 EELGSDEDDIDEDGQEYLEILAKQAGEDG--DDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQAIQSRN sacHar7 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIF EE+ SDE+D +E Q E ++ +D++W+ED EETALEG+ST +D E++ VDEYQ F sacHar8 EEIPSDEEDTNETSQTMHENNGGGDEDEEEDDDWDEDVLEETALEGFSTPLDLEDS-VDEYQFF
Homoplasy (recurrent mutation) issues: It can be seen from the 44-species alignment that the serine here is quite invariant, being conserved in all amniotes. In frog and all earlier diverging species, threonine is utilized. However serine is used in all vertebrates at the comparable position in the paralog IPO8 except for tetraodon which again uses threonine, as do weaker homologs in the protostomes Tribolium and Ixodes and cnidarians Nematostella and Acropora. This could be described as a reduced alphabet situation where the residue is strongly restricted to a small residue with hydroxyl side chain. Phenylalanine here, as in Sarcophilus, is thus an immensely non-conservative change as it is bulkly, unable to hydrogen bond, and unsuitable for the protein surface.
Query 2 ELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYFTIIDDEENPVDEYQIFKAIFQ IPO7_sarHar EL SDED+I+ED +Y+E LA +A E DD++ E EETALE + T +D EE +DE+ F+ Q Sbjct 1788 ELASDEDEINEDDVQYIESLALKAAEHLDDDDVCE---EETALENFTTSVDTEE--IDEFIAFRTSLQ Acropora millepora Query 3 LGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYFTIIDDEENPVDEYQIFK IPO7_sarHar L SDED+ +ED EY+E LAK+A D D+E ++DD EET LE Y T ID E +DEY FK Sbjct 2611 LASDEDEFNEDDVEYIENLAKKAA-DHFDDEDDDDDDEETPLEEYTTSIDGEN--MDEYIAFK Nematostella vectensis
Known variations: No disease variants are known according to OMIM for either IPO7 or IPO8. No relevent structure at PDB has been determined for the central or distal region of the protein. The protein is quite large and thus it will be very difficult to predict the environment of the serine, much less the impact of phenylalanine substitution.
Side issues: Importin IPO7 has a broad and extremely important function in nuclear protein import, either autonomously as nuclear transport receptor or as an adapter in association with KPNB1. Havilng a receptor for nuclear localization signals, it can promote translocation of import substrates through the nuclear pore complex (NPC) by the energy requiring RAN-dependent mechanism. It mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5, but in association with KPNB1 the import of five histones. The role of the paralog IPO8 is similar.
The question here is to what extent could IPO8 compensate for the S-->F change observed in Sarcophilus. It seems implausible given the divergence of the two proteins and the great conservation of IPO7 in the enveloping exon -- what selective force could maintain this if an auxillary gene is available to take on the nuclear import role?
^ ^
IPO7_homSap EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ IPO7_panTro EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_ponAbe EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_rheMac EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_calJac EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_tarSyr EELGSDEDDIDEDGQEYLEILAKQAGEDGDEEEWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ................................E.....................E.............. IPO7_micMur EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_tupBel EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_musMus EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_ratNor EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_dipOrd EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_cavPor EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_speTri EELGSDEDDIDEDGQEYLEILAKQAGEDGDDDDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ...............................D..................................... IPO7_oryCun EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_ochPri EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_vicPac EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_turTru EELGSDEDDIDVDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ...........V......................................................... IPO7_bosTau EELGSDEDDIDEDGQEYLEILAKQAGEDGDEEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..............................E...................................... IPO7_equCab EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_canFam EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_myoLuc EELGSDEDDIDEDGQEYLEILAKQAGEDGDDDEWEENDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ...............................DE...N................................ IPO7_pteVam EELGSDEDDIDEDGQEYLEILAKQA-EDGDDEDWR-DDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ .........................-........R-................................. IPO7_eriEur EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_sorAra EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_loxAfr EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_proCap EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_echTel EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_dasNov EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_choHof EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_monDom EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ ................................E.....................E.............. IPO7_sarHar1 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ ................................E.....................E.............. IPO7_sarHar2 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYFTIIDDEENPVDEYQIFKAIFQ ................................E..............F......E.............. IPO7_ornAna EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ ..................................................................... IPO7_galGal EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKTIFQ .................................................................T... IPO7_taeGut EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPIDEYQIFKTIFQ .........................................................I.......T... IPO7_anoCar EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPIDEYQIFKAIFQ .........................................................I........... IPO7_xenTro AELGSDEDDIDEEGQEYLEILAKQAGEDGDDEDWEDDDAEETALEGYTTLIDDEDTPIDEYQIFKAIFQ A...........E......................D...........T.L.....T.I........... IPO7_tetNig AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDEDWEEDDAEETALEGYTTTVDDEDNFVDEYQIFKAILQ A...........E......M...........................T.TV.....F..........L. IPO7_takRub AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDEDWEDDDAEETALEGYTTNIDDEDNFVDEYQIFKAILQ A...........E......M...............D...........T.N......F..........L. IPO7_gasAcu AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDEDWEEDDAEETALEGYTTAVDDEDNLVDEYQIFKAILQ A...........E......M...........................T.AV.....L..........L. IPO7_oryLat AELGSDEDDIDEEGQEYLEMLAKQAGEDGDDDDWEEDDAEETALEGYTTAIDDEDNFVDEYQIFKAVLQ A...........E......M...........D...............T.A......F.........VL. IPO7_danRer AELGSDEDDIDDEGQEYLEMLAKQAGEDGDDEDWEEDDAEETALEGYTTLVDDEDNLVDEYQIFKAIMQ A..........DE......M...........................T.LV.....L..........M. ^ ^ ^ IPO8_hg18_23 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI IPO8_panTro2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI IPO8_gorGor1 -EISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI IPO8_ponAbe2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI IPO8_rheMac2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI IPO8_calJac1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDEDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALI IPO8_tarSyr1 EEISSDEEETTVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPIDLDHSVDEYQFFTQALL IPO8_micMur1 -EIASDEEEMNVNAQAMQSSNGRGEDEEEDDDDWDDEVVEETALEGFSTPLDLDSSVDEYQFFTQALL IPO8_otoGar1 KEISSDEEESNVKAQAMQSNNGRGDDEEEEEDDWDEEVLEETALEGFSTPLDLDSSVDEYQFFTQALL IPO8_dipOrd1 EEISSDEEEKSVSVQAMQSVNRRGADEEDEDEDWEEEILEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_cavPor3 EEISSDEEETNANAQAMQSNTRKG--EEEEDDDWDEEVLEETALEGFSTPLDLDDSVDEYQFFTQALL IPO8_speTri1 EEISSDEEDTNITAQAMQANNGRSGDEEEEQDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_oryCun1 EEISSDEEETNVASQAVQSSSGRGEDEEEDDDDWADEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_ochPri2 -EISSDEEETNPSTQAMQSSTGRGEDEDEEEEEWDDEVLEETALESFSTP----ECVDEYQFFTQALL IPO8_vicPac1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_turTru1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_bosTau4 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_equCab2 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_felCat3 EEISSDEEETNVTAQAMQSNNGRGEDEEEEEDDWDEEVLEETALEGFSTPLDLDNSVDEYQIFTQALL IPO8_canFam2 EEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_myoLuc1 EEISSDEEEANITAQAMQSKNGRGEEEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALL IPO8_pteVam1 E-ISSDEE-ANVTAQAMQPNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYLFFTQALL IPO8_eriEur1 EEISSDEEETTVGVQAKQPSNGRVEAEEDDDDDWEEELLEETTLEGFSTPLDLDGSVDEYQFFTQALL IPO8_loxAfr2 -EISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDSSVDEYQFFTQALL IPO8_proCap1 EEISSDEEETNVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDSSVDEYQFFTQALL IPO8_echTel1 EEISSDEEETNVTAQAMQSTNGRGDNEEEEEDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFAQALL IPO8_choHof1 EEISSDEEETSVTAQAMQSNNGRGEDEEEDDDDWDEEVLEETALEGFSTPLDLDSNVDEYQFFTQALL IPO8_monDom4 EEIPSDEEDTNEARQAL--S-GGGEDEEEDDDDWDEEVLEETALEGFSTPLDLDDGVDEYQFFTQALL IPO8_ornAna1 EEIPSDEEETNETGQLMQENLGGDEEEDDEDDDWDEDVLEETALEGFSTPLDLENSVDEYQFFTQALL IPO8_galGal3 EEIPSDEEETNEVSQAMQENHGEEEDDDDDDDDWDEDALEETALEGFSTPLDLENGVDEYQFFTQALL IPO8_taeGut1 EEIPSDEDETNEVSQAMQENHGEEEDEDDDDDDWDEDALEETALEGFSTPLDLENGVDEYQFFTQALL IPO8_anoCar1 EEIPSDEEEANEVTQEMQENHVGDEDDDDDDDDWDDDALEETALEGFSTPIDLEDAVDEYQFFTQALI IPO8_xenTro2 EEIASDEEEAN---QAMQQN---GEDAEEEDEDWDDEVLEETALEGFSTPLDCEDALDEYQFFTNALL IPO8_tetNig1 QEIPSDEDEVNENH-A-QQASRNGAEDEEEDDYWEDDCFEGTALEEYTTPLDFDNGEDEYLFFTSTLL IPO8_fr2_23_ QEIPSDEDEVSENHSA-PLPNMSGEDDEEEDDYWDDDGFEGTPLEEYSTPLDFENGEDEFHFFTSTLL IPO8_gasAcu1 QEIPSDEDEVTENRKAVQHANR-EEEEEDDEDDWDNDCFEGTPLEEYSTPLDYDNGEDEYQFFASALL IPO8_oryLat2 EEIPSDEDEVNENREAVQHHSR-EDDDDDEEDYWEEDGFEGTPLEEYSTSLDYDNGEDEYEFFTCALL IPO8_danRer5 EEIPSDEDEVGEKGVAIRRSHREDDDDEDDDEYWDDEGLEGTPLEEYSTPLDCDNGEDEYQFFTASLL ^ ^ >IPO7_homSap MDPNTIIEALRGTMDPALREAAERQLNE AHKSLNFVSTLLQITMSEQLDLPVRQA GVIYLKNMITQYWPDRETAPGDISPYTIPEEDRHCIRENIVEAIIHSPELIR VQLTTCIHHIIKHDYPSRWTAIVDKIGFYLQSDNSACWLGILLCLYQLVKNYE YKKPEERSPLVAAMQHFLPVLKDRFIQLLSDQSDQSVLIQKQIFKIFYALVQ YTLPLELINQQNLTEWIEILKTVVNRDVPN ETLQVEEDDRPELPWWKCKKWALHILARLFER YGSPGNVSKEYNEFAEVFLKAFAVGVQQ VLLKVLYQYKEKQYMAPRVLQQTLNYINQGVSHALTWKNLKPHIQ GIIQDVIFPLMCYTDADEELWQEDPYEYIRMKF DVFEDFISPTTAAQTLLFTACSKRKE VLQKTMGFCYQILTEPNADPRKKDGALHMIGSLAEILLK KKIYKDQMEYMLQNHVFPLFSSELGYMRAR ACWVLHYFCEVKFKSDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEK AKEYITPFIRPVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHL AMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKE ITQQLEGICLQVIGTVLQQHVL EFYEEIFSLAHSLTCQQVSPQMWQLLPLVFEVFQQDGFDYFT DMMPLLHNYVTVDTDTLLSDTKYLEMIYSMCKK VLTGVAGEDAECHAAKLLEVIILQCKGRGIDQ CIPLFVEAALERLTREVKTSELRTMCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLG 1 LHDRKMCVLGLCALIDMEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDDDDEAEDDDET 1 2 EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQ 1 2 TIQNRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAH 1 ESKMIEKHGGYKFSAPVVPSSFNFGGPAPGMN* >IPO7_monDom MDPNTIIEALRGTMDPALREAAERQLNE AHKSVNFVSTLLQITMSEQLDLPVRQA GVIYLKNMITQYWPDRETTPGEIPPYTIPEEDRHCIRENIVEAIIHSPELIR VQLTTCIHHIIKHDYPSRWTAVVDKIGFYLQSENSACWLGILLCLYQLVKNYE YKKPEERSPLVAAMQHFLPVLKDRFIQLLPDQSDQSVLIQKQIFKIFYALVQ YTLPLELINQANLTEWIEILKTVVNRDVPP ETLQVEEDDRPELPWWKCKKWALHILARLFER YGSPGNVSKEYNEFAEVFLKAFAVGVQQ VLLKVLYQYKEKQYMAPRVLQQTLNYINQGVSHAVTWKNLKPHIQ GIIQDVIFPLMCYTDADEELWQEDPYEYIRMKF DVFEDFISPTTAAQTLLFTACSKRKE VLQKTMGFCYQILTEPNADPRKKDGALHMIGSLAEILLK KKIYKDQMEYMLQNHVFPLFSSDLGYMRAR ACWVLHYFCEVKFKSDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEK AKEYITPFIRPVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHL AMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKE ITQQLEGICLQVIGTVLQQHVL EFYEEIFSLAHSLTCQQVSPQMWQLLPLVFEVFQQDGFDYFT DMMPLLHNYVTVDTDTLLSDTKYLEMIYSMCKK VLTGVAGEDAECHAAKLLEVIILQCKGRGIDQ CIPLFVEAALERLTREVKTSELRTMCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLG LHDRKMCVLGLCALIDLEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDEDDEADDDEET EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ AIQSRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAH ESKMIEKHGGYKFNAPVVPSSFNFGGPAPGMN* >IPO7_sarHar LHDRKMCVLGLCALIDLEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDEDDEADDDEET EELGSDEDDIDEDGQEYLEILAKQAGEDGDDEEWEEDDAEETALEGYSTIIDDEENPVDEYQIFKAIFQ AIQSRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAH >IPO8_sarHar EEIPSDEEDTNETSQTMHENNGGGDEDEEEDDDWDEDVLEETALEGFSTPLDLEDS-VDEYQFF
Case of PPFIA3
chr4_22002 PPFIA3 15 'anomalous mapping from monDom5 to human' >contig00001 length=298 numreads=4 LIQEEKETTEQRAEELESRVSGSGLDSLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNTAEKP ........................................................F..................G.V. ^ 56 F=2(43) S=2(37) Here both individuals differed from Monodelphis by S->F at position 56 of PPFIA3 with a confusing end to the exon.
Pseudogene issues: Not applicable.
Paralog issues: PPFIA3 (liprin) has 3 paralogs with considerable (but readily differentiable) sequence identity in this exon. These latter genes are more similar to each other than to PPFIA3, yet all 4 have S at the position occupied by F in tasmanian devil. The ancestral gene duplications must be quite old because lamprey has at least two copies and PPFIA3 itself is readily traced to shark.
PPFIA3 is missing in chicken and finch (proving it is not an essential gene in vertebrates) though present in lizard and frog. These latter species have a one residue mid-exon insert relative to mammals compensated for well past the key residue with a one residue deletion. All three species of marsupials with available data have an 8 residue insert three residues from the end of the exon (which still ends in phase 0 like all other orthologs). These indels have seriously affected the UCSC 44-species alignment quality. The batch of sequences immediately below are hand-curated directly from trace reads but otherwise are provided 'as is.'
The S-->F changed observed in tasmanian devil is likely very significant to protein function given the immense conservation of this residue and its flanking environment. However given the numerous independent indels still within this exon -- especially the 8 residue insert in the marsupial stem -- it would be difficult to argue that S-->F could not somehow be compensated with material impact on function. The complete loss of the gene in two birds (together these species have overwhelming trace coverage and many transcripts) establishes either that PPFIA3 lost its importance important or that one of its three paralogs can assume its function.
No structural data relevent to this exon exists at PDB. The entry at SwissProt shows two predicted phosphoserines within the exon but not at the serine here. Predicted domains and secondary structure coils are not applicable to this exon either. The function is somewhat understood: it may regulate the disassembly of focal adhesions, localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane and forms homodimers and heterodimers with other liprins.
p * p homSap LIQEEKETTEQRAEELESRVSSSGLD-SLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGT--------DKA sarHar LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRASCSLPPSLTTSTLASPSPPSSGHFTPRPAPPSPAREAPANSTGNVADKP monDom LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNTAEKP macEug LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNAADKP ornAna LIQEEKETTEQRAEELESRVSGSGLD-SLGRYRGGSALPASLTSSTLASPSPPSSGHSTPRLAPPSPAREGS--------EKT anoCar LIQEEKESTEQRAEEIESRVTSASLDGSLGRYRSGASIPPSVTSSTLASPSPPSSGHSTPRLAPHSPARDG---------EKM xenTro LIQEEKETTELRAEEIESRVTSGTLDGSLGRYRSASSIPTSVTTSTLASPSPPSSGHSTPRITPHSPAREG---------DKF PPFIA3 monDom LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTL PPFIA2 monDom LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSLSSLPPHPSSCLSG--SSPPGSGRSTPRRHPHSPAREVDRLGIMTL PPFIA1 monDom MIQEEKESTELRAEEIETRVTSGSMEALNLQLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4
Homoplasy (recurrent mutation) issues:
Known variations:
Side issues:
* >PPFIA3_sarHar FHGHM9L01BYK1T length=435 xy=0686_3455 region=1 aggcttatccaggaggaaaaggagaccacggagcagcgggccgaggaactggagagccgc L I Q E E K E T T E Q R A E E L E S R gtgtccggctctggcctggactccctgggacgctaccgggccagctgctccctcccgcct V S G S G L D S L G R Y R A S C S L P P tccctgaccacgtccaccctggctagcccttccccccccagctctgggcactccacgccc S L T T S T L A S P S P P S S G H S T P cgccctgctccccccagtcccgcccgggaagccccggccaacagcaccggcaacgtggca R P A P P S P A R E A P A N S T G N V A gataagcccgtgagt D K P >PPFIA3_monDom phase 0 ggccactccacccctcgccctgccccgcccagccctgctcgggaagctccagccaacagcactagcaacactgcagaaaagcctgtgagt G H S T P R P A P P S P A R E A P A N S T S N T A E K P V S >PPFIA3_macEug Macropus eugenii phase 0, assembly has early frameshift due to extra G aggctcatccaggaggagaaggagacgacggaacagcgggcagaggagctggagagccgg R L I Q E E K E T T E Q R A E E L E S R gtgtctggctctggcctggactccttgggacgctaccgggccagctgctcccttccacct V S G S G L D S L G R Y R A S C S L P P tccctgactacatccaccctggccagcccttcaccccccagctctggtcactccacaccc S L T T S T L A S P S P P S S G H S T P cgccctgccccacccagccctgcccgagaagccccagccaacagcactagcaacgctgca R P A P P S P A R E A P A N S T S N A A gataagcctgtgagt D K P V S >PPFIA3_xenTro aggttaatccaagaggaaaaggagacaacagagttgcgggctgaagaaatagagagtcga L I Q E E K E T T E L R A E E I E S R gtgaccagcggcactctggacggatcactgggacgctaccgttctgccagttccatcccc V T S G T L D G S L G R Y R S A S S I P acctccgtcaccacatcaactctagccagtccctcaccacccagcagtgggcattccacc T S V T T S T L A S P S P P S S G H S T ccgcgcatcacgccacacagccctgccagagaaggagacaaatttgtaagttcctttcaa P R I T P H S P A R E G D K F V S >PPFIA3_ornAna platypus phase 0 ctgatccaggaggaaaaggagacgacagagcagcgggccgaggagctggagagccgggtg L I Q E E K E T T E Q R A E E L E S R V tccggctcggggttggactccctgggccggtaccggggcggcagtgccctgcccgcctcc S G S G L D S L G R Y R G G S A L P A S ctcacctcctccaccctggccagcccctctccccccagcagcggccactccaccccccgc L T S S T L A S P S P P S S G H S T P R ctggcgccccccagccccgcccgcgaggggtccgaaaaaaccgtaagtggaaaaggccgc L A P P S P A R E G S E K T >PPFIA3_anoCar aggttgatccaggaggaaaaagaatccacagaacaacgggcagaggaaatcgagagccga L I Q E E K E S T E Q R A E E I E S R gtgactagtgccagcttggacggttccctcggccgctaccgctcaggcgcttccatccct V T S A S L D G S L G R Y R S G A S I P ccctccgtcaccagctccaccctggccagcccttctccccccagcagtggccactccacc P S V T S S T L A S P S P P S S G H S T ccccgcttggcgccccatagccctgctcgcgatggggaaaaaatggtatgtcatgactgt P R L A P H S P A R D G E K M >PPFIA3_homSap phase 0 aggctgatccaagaggagaaggagacaacagaacagagggcagaggagctggagagtcgg R L I Q E E K E T T E Q R A E E L E S R gtgtccagctctggcttggactcgttgggccgctaccgcagcagctgctccctgcccccc V S S S G L D S L G R Y R S S C S L P P tccctcaccacctctacccttgccagcccctcccctcccagctctggccactcaacaccc S L T T S T L A S P S P P S S G H S T P cgcctggcaccccctagccctgcccgtgagggcaccgacaaggctgtgagtgctctgaag R L A P P S P A R E G T D K A V S A L K tctccccagcctagt S P Q P S >PPFIA3_calMil Callorhinchus milii MIQEEKETNELRAEEIESRVGSGTLEGPQGGGYRSAASLSHSVTASTLASPSPPNSGHSPRMAPHSPAREGDRVGIGNTVS atggcacctcacagcccagccagggagggggacagggtcggcatcggcaacacagtgagt M A P H S P A R E G D R V G I G N T V S PPFIA3_homSap LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_panTro LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_rheMac LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_calJac LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_tarSyr LIQEEKDTTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT PPFIA3_micMur LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_mm9_15_ LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT PPFIA3_rn4_15_ LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPARE-TDKT del verified PPFIA3_dipOrd LIQEEKETTEQRAEELESRVSSSGLDSLSRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_cavPor LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_ochPri LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_turTru LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_bosTau LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_equCab LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_canFam LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_sorAra LIQEEKETTEQRAEELESRVSGSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT PPFIA3_loxAfr LIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKA PPFIA3_proCap LIQZEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKT PPFIA3_monDom LIQEEKETTEQRAEELESRVSGSGLDSLGRYRASCSLPPSLTTSTLASPSPPSSGHSTPRPAPPSPAREAPANSTSNTAEKP PPFIA3_ornAna LIQEEKETTEQRAEELESRVSGSGLDSLGRYRGGSALPASLTSSTLASPSPPSSGHSTPRLAPPSPAREGSEKT PPFIA3_anoCar LIQEEKESTEQRAEEIESRVTSASLGSLGRYRSGASIPPSVTSSTLASPSPPSSGHSTPRLAPHSPARDGEKM PPFIA3_galGal missing PPFIA3_taeGut missing PPFIA3_xenTro LIQEEKETTELRAEEIESRVTSGTLGSLGRYRSASSIPTSVTTSTLASPSPPSSGHSTPRITPHSPARE--DKF PPFIA3_tetNig LIQEEKENTELRAEEIENR--SVALATLGRDAAGRFLPSSITSSTLASPSPPSSGHSTPRL-PHSPAREPSDR- PPFIA3_fr2_15 LIQEEKESTELRAGEIESRVSSVALASLGRDSIGRYMTPSITSSTLASPSPPSSGHSTPRL-PHSPARETTDR- PPFIA3_gasAcu LIQEEKENTELRAEEIESRVSSVALASLGGDSVGRYMTPSITSSTLASPSPPSSGHSTPRL-PHSPARETTDR- PPFIA3_oryLat LIQEEKENTELRAEEIESR--SVALASLGRDSAGRFIPSSITSSTLASPSPPSSGTSTPRL-PHSPAREMTDR- PPFIA3_danRer LIQEEKESTELRAEEIESRVSSVALASLGRDSTGRFIPPSLTSSTLASPSPPSSGHSTPRL-PHSPARETTDR- PPFIA2_homSap LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_panTro LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_ponAbe LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_rheMac LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_calJac LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_tarSyr LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_micMur LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_otoGar LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_tupBel LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_mm9_16 LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_rn4_16 LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_cavPor LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_speTri LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_oryCun LIQEEKESTELRAEEIENRVASVSLEGLNLARVHQGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_vicPac LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_turTru LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_bosTau LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_equCab LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_felCat LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_canFam LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTTITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_pteVam LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_eriEur LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_sorAra LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_proCap LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_echTel LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_dasNov LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_choHof LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_monDom LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTL PPFIA2_ornAna LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_galGal LIQEEKESTELRAEEIENRVASVSLEGLNLARVHQGTSITGSVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_taeGut LIQEEKESTELRAEEIENRVASVSLEGLNLARVHQGTSITGSVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_anoCar LIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASITASSLASSSPPSGHSTPKLTPRSPAREMDRMGIMTL PPFIA2_xenTro LIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTL PPFIA2_tetNig LIQEEKESTELRAEEIEHRVASVSLEGLNLARIHHGASITASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL PPFIA2_fr2_16 LIQEEKESTELRAEEIENRVASVSLEGLNLARIHHGASITASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL PPFIA2_gasAcu LIQEEKESTELRAEEIENRVASVSLEGLNLARIHHGASITASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL PPFIA2_oryLat LIQEEKESTELRAEEIENRVASVSLEGLNLARIHHGVSMTASATASSLASSSPPSGHSTPKLDPRSPARDMERMGVMTL PPFIA2_danRer LIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASATASSLASSSPPSGHSTPKLTPRSPARDMERMGVMTL PPFIA1_homSap LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_panTro LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_gorGor LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_ponAbe LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_rheMac LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_calJac LIQEEKENTEQRAEEIENRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPSSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_tarSyr LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRPVSSIPPCPASSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_micMur LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLGGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_otoGar LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_tupBel LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRMPHSPAREVDRLGIMTL PPFIA1_mm9_15_ LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTL PPFIA1_rn4_15_ LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTL PPFIA1_dipOrd LIQEEKESTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRVPHSPAREVDRLGVMTL PPFIA1_cavPor LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRSTPRMVPHSPAREVDRLGVMTL PPFIA1_speTri -------------EEIESRVGSGSLDNLGRFRSMSSLPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTL PPFIA1_turTru LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSVSSIPPYPASSRASSSPPSSGRPTPRRAPHSPAREVDRLGVMTL PPFIA1_bosTau LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSMSSIPPYPASSLAGSSPPSSGRSTPRRMPHSPAREVDRLGIMTL PPFIA1_equCab LIQEEKENTEQRAEEIESRVGSGSFGNLR-FRSVSSIPLYPASSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_felCat LIQEEKESAEQRAEEIESRVGSVFLDSPGRFRPAGSGAPHPASPLAGPSPPHSGRSTPRRGPHSPAREVDRLGVMTL PPFIA1_canFam LIQEEKESTEQRAEEIESRVGSGSLDSPGRFRSLGDAPPHPTSVLTGPSPPHSGRSTPRRGPHSPAREVDRLGVMTL PPFIA1_myoLuc LIQEEKESTEQRAEEIESRVGSGSLDNLGRFRSMSS--PYPGSSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_pteVam LIQEEKENTEQRAEEIESRVGSGSLDSLGRFRSMSAIPPYPASSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_sorAra LIQEEKESTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPNSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_loxAfr LIQEEKESAEQRAEEIESRVGSGSLDNLDRFRSMSSIPPYPAPSLAGSSPPGSGRSTPRRIPQSPAREVDRLGIMTL PPFIA1_proCap LIQEEKESAEQRAEEIESRVGSGSLDNLDRFRSVSSIPPYPASSLAGSSPPGSGRSTPRRIPQSPAREVDRLGIMTL PPFIA1_echTel LIQEEKENAEQRAEEIESRVGSGSLSDLGHFRPLGSVPPHPSSALAGSSPPGSGRSTPRRIPQSPSREVDQLGIMTL PPFIA1_dasNov LIQEEKENTEQRAEEIESRVGSGTLDNLGRFRSLSSIPPYPASSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_choHof LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSLSAIPPYPASSLASSSPPGSGRSTPRRMPHSPAREVDRLGVMTL PPFIA1_monDom LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSLSSLPPHPSSCLSGSSPPGSGRSTPRRHPHSPAREVDRLGIMTL PPFIA1_ornAna LIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPGSSLAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTL PPFIA1_galGal LIQEEKENTEQRAEEIESRVGSGSLDAHGRFRSMSSIPPPYGGSLAGSSPPGSGRSTPRRIPHSPTREVDRLGIMTL PPFIA1_taeGut LIQEEKENTEQRAEEIESRVGSGSLEAHGRFRSLGSIAPALGGALAGSSPPGSGRSTPRRIPHSPAREVDKLGIMTL PPFIA1_anoCar LIQEEKENTEQRAEEIESRVGSGSLENLGRFRSMSSLPAPFRGSLSGTSPPGSGRSTPRRMPHSPAREVDRLGIMTL PPFIA1_xenTro LIQEEKETTEQRAEEIESRVGSGSLDNLGRFRSITSIPPFTGTSLAGSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA1_tetNig MIQEEKESTAIRAEEIECRVGSEGLG--GRFRSMSSIPPCMGSSLGG-SPPGSGHSTPRRIPCSPNRELDRMGVMTL PPFIA1_fr2_15 MIQEEKESTAIRAEEIECRVGSDGLG--GRFRSMSSIPPCMGSSVGG-SPPGSGHSTPRRIPRSPNRELDRMGVMTL PPFIA1_gasAcu MIQEEKENTVIRAEEIECRVGSDSLG--GRFRSMGSIPPCPGSSLGG-SPPGSGHSTPRRVPRSPNRELDRMGVMTL PPFIA1_oryLat MIQEEKESTAIRAEEIECRVGSDSIG--GRFRSLSSIPPCAGSSLGG-SPPSSGHSTPRRIPRSPNRELDRMGVMTL PPFIA1_danRer LIQEEKESTELRAEEIENRVASVSLE--GRIWHESTIPPSTASSLAS-SSPPSGHSTPKLTPRSPARDMERMGVMTL PPFIA1_petMar LIQEEKESTEQLAEEIEIRVGGSSGGGGGRLRSARSIPGSATATLATNSAPVSGYATPKRLTHSPAHDPDRHGAMTL PPFIA4_homSap MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_gorGor MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTAKLTSRSAAQDLDRMGVMTL PPFIA4_ponAbe MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_rheMac MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_calJac MIQEEKESTELRAEEIETRVTSGSMEALNLKQLRKRGSIPTSLTALSLASTSPPLSGRSTPKLTSRSAAQGLDRMGVM-- PPFIA4_micMur MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_tupBel MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_musMus MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_ratNor MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_dipOrd MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPSLSGRSTPKLTSRSPAQDLDRMGVMTL PPFIA4_cavPor MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGIMTL PPFIA4_ochPri MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSTAQDLDRMGVMTL PPFIA4_vicPac M-QEEKESTELRA-EIDTEVTSGSLEVLKLXLKLQCGGI------------SPPLSGRSAPKLTSRSAAQDLDRMGVMTL PPFIA4_turTru MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPFSGRSTPKLTSRSATQDLDRMGVMTL PPFIA4_bosTau MIQEEKESTELRAEELETRVTSGSMEALDLTQLHKRGSIPTSLTALSLASASPPLSGRATPKLTSRSAAQDLDRMGVMTL PPFIA4_equCab MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_felCat MIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_canFam MIQEEKESTELRAEEIETRVSSGSVEALNLTQLRKRGSIPTSLTALSLASASPPLSGRSTPKLASRSAAQDLDRMGVMTL PPFIA4_myoLuc MIQEEKESTELRAEEIETRVTSGSMEALNLTQPHRRGPIPTSLTALSLASGSPAFSGRSTAKCASRSAVQDLDRMGVMTL PPFIA4_eriEur MIQEEKESTELRAEENETRVTSGSMEALNLSQRRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_sorAra MILEEKEATELRAEEIETRMNSASIE-LDSSQLRKRASITTPZMPLSLARASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_loxAfr MIQEEKESTELRAEEIETQVTSGSMEALNL-QLRKRASIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_proCap MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRASIPTSLTALSLASTSPQLSGRSTPKLTSRSTAQDLDRMGVMTL PPFIA4_echTel MIQEEKESAELRAEEIETRVTSGSMEALNL-QLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_dasNov MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_choHof MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRGSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_monDom MIQEEKESTELRAEEIETRVTSGSMEALNL-QLRKRSSIPTSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTL PPFIA4_ornAna LIQEEKESTELRAEEIENRVASVSLEGLNL-RVHPGTSITASVTASSLASSSP--SGHSTPKLTPRSPAREMDRMGVMTL PPFIA4_galGal MIQEEKESTELRAEELETRVTSGSMEGLNL-QLCKRASIPTSLTALSLASSSPPLSGRSTPKLTSRSAAQDLDRMGIMTL PPFIA4_taeGut MIQEEKESTELRAEELETRVTSGSMEGLNL-QLCKRASIPTSLTALSLASSSPPLSGRSTPKLSSRSAAQDLDRMGIMTL PPFIA4_anoCar MIQEEKESTELRAEQLESRVTSGSMEALNL-QLRKRASIPTSLTALSLASSSPPISGRSTPKLSSRSAAQDLDRICSMTL PPFIA4_xenTro LIQEEKETTEQRAEEIESRVGSGSLDNLG---FQVHHFNSPFZVV-SLAGSSPPGSGRSTPRRIPHSPAREVDRLGVMTL PPFIA4_tetNig LIQEEKESTELRAEEIEHRVASVSLEGLNL--PPPRR--PASATASSLASSSP--SGHSTPKLDPRSPARDMERMGVMTL PPFIA4_takRub MIQVERESADLRSDEIESRVNSGSMDGLNV--LRPRA--PTSATAQSLASSCSPHSGHSTPKHHSRNAGHH---LGIMTL PPFIA4_gasAcu MIQVERESADLRSDEIESRVNSGSMDGLNV--LRPRA--PTSATAQSLASSSSPPSGHSTPKHHSRNASHH---LGIMTL PPFIA4_oryLat MIQVERESADLRSGDIESRVNSGSMDGLNV--LRPRA--PTSATAQSLASSSSPHSGHSTPKHHGRNASHH---LGIMTL PPFIA4_danRer MIQVERESAELRADEIESRVNSGSMDGLNV--LRPRSSIPTSVTALSLASSSP--SGRSTPKLTSGSTAHE---LGIMTL PPFIA4_petMar LIQ-EKESTEQRAEEIESRVGSGSLDSLSL-QQRDGGSLPVSLTGSSLASSSPPVSGRSTPKFTPRSPARDADRAGA---
Case of WDFY3
chr5_2532 WDFY3 19 >contig00001 length=482 numreads=8 DDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK ................T..............................T..L.....N... ^ 16 T=3(117) A=5(138) Tasmanian devil differs from Monodelphis by A->T at position 16. The variation observed later in the exon is largely in opossum rather than other marsupials and occurs at positions with tight reduced alphabets.
Pseudogene issues: None.
Paralog issues: Some weak paralogs exist in human. These are poorly conserved in the region in question even about the key residue. While this weakens the overall invariance of the key residue, it also eliminates any possibility of cross-alignment to inappropriate homologs.
WDFY3 0 WD repeat and FYVE domain containing 3 isoform WDFY4 0 WDFY family member 4 LYST 3e-114 lysosomal trafficking regulator NBEAL1 6e-109 neurobeachin-like 1 isoform 1 NBEAL2 7e-109 neurobeachin-like 2 LRBA 5e-100 LPS-responsive vesicle trafficking, beach and NBEA 3e-98 eurobeachin NSMAF 1e-78 neutral sphingomyelinase (N-SMase) activation
Homoplasy (recurrent mutation) issues: None.
Known variations: Not a known disease gene; no relevent human variants known.
Side issues: None.
Structural significance: WDFY3 encodes a very large peripheral membrane protein of 3526 aa and 65 codinbg exons containing two leucine-rich repeats, a BEACH doman, five WD domains, a FYVE-domain,3 phosphotyrosines, 2 phosphoserine, and 1 phosphothreonine. However none of these are immediately relevent to the three exons centered on the SNP-containing exon. SuperFamily identifies the key exon significant matches (4e-09) as a concanavalin A-like lectin/glucanase domain. It co-localizes with autophagic structures in starved cells. The few transcripts that cover this region arise from testes (Xenopus), heart (chicken), early embryo (pig), and colon and hypothalamus (human), not informative as to function.
Functional significance: The substitution of threonine for alanine in proteins in general has quite mild effects. Alanine is the most generic amino acid and never catalytically active; threonine is polar but not charged and only somewhat bulkier. However the comparative genomics of this alanine in WDFY3 says this alanine is very different -- it is completely invariant over immense branch length back to chondrichthyes with the sole exception of Sarcophilus.
The embedding exon has more nearby variability than some of the other candidates. Its rather diverged paralog WDFY4 has leucine in place of alanine; this leucine is quite well conserved but has some exceptions. Note the alanine is not one of the better conserved residue patches in the overall region. Thus it appears that the substitution A-->T will have a significant effect on function but not a catastrophic one on core properties.
WDFY3 VSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVKLHYVHSTPG VST+E+ Q +D E C + RCG+L+ GQWHHL +V++K M ++ T + +DGQ++ + K+ Y+ + PG WDFY4 VSTEEKEFQP-LDVMEPEDDSEPSAGCQLQVRCGQLLACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAKMLYIQALPG * * homSap VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK homSap panTro VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. panTro gorGor VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. gorGor ponPyg VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. ponPyg macMul VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. macMul calJac VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ............................................................. calJac otoGar VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK .......................D..V.................................. otoGar musMus VDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYLDGQLVSTVK .........................VV.......A.L..R...........L.....S... musMus ratNor VDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYIDGQLVSTVK .........................VV.......A.L..R.................S... ratNor dipOrd VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYLDGQLVSTVK ..........................V........................L.....S... dipOrd cavPor VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLALVMSKGMLKNSTATLYIDGQLVSTVK ................................................T........S... vicPac speTri VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. speTri ochPri VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK ..........................V..............................S... ochPri vicPac VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTATLYIDGQLVSTVK ..........................V.....................T........S... bosTau turTru VDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSRGMLKNSTAALYIDGQLVSTVK .......................................R.................S... turTru bosTau VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTATLYIDGQLVSTVK .......................D..V........................L......I.. taeGut equCab VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. equCab felCat VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. felCat canFam VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. canFam echTel VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVK ..........................V.................................. echTel dasNov VDDFSEESSFYEILPCCARFRCGELIVEGQWHHLVLVMSKGMLKNSTAALYIDGQPVTTVK ..........................V............................P.T... dasNov choHof VDDFSEESSFYEILPCCAHFRCGELIVEGQWHHLVLVMSRGMLKNSTAALYIDGQLVNTVK ..........................V.......A.............T........S... cavPor monDom VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK .......................D..V..............................S... monDom macEug VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYLDGQLVNTVK .......................D..V........................L......... macEug sarHa1 VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTATLYLDGQLVNTVK .......................D..V.....................T..L......... sarHar sarHa2 VDDFSEESSFYEILPCCTRFRCGDLIVEGQWHHLVLVMSKGMLKNSTATLYLDGQLVNTVK .................T.....D..V.....................T..L......... sarHar ornAna ADDFSEESSFYELLPCCAHFRCGDLIAEGQWHHLVLVMSKGMLKNSTATLYIDGQLVNTVK A...........L.....H....D..A.....................T............ ornAna galGal VDDFSEESSFYEILPCCARFRCGELIAEGQWHHLVLVMSKGMLKNSTAALYLDGQLVNTVK ..........................A........................L......... galGal taeGut VDDFSEESSFYEILPCCARFRCGDLIVEGQWHHLVLVMSKGMLKNSTAALYLDGQLVNIVK ..................H.......V............R..................... choHof anoCar VDDFGEESSCYEILPCCARFRCGDHIVEGQWHHMVLVMSKGMLKNSTAALYIDGQLINTVK ....G....C.............DH.V......M......................I.... anoCar xenTro VDDFSEEASFYEILPCCARFRCSDLIMEGQWHHLVLVMSKGMLKNSTAALYIDGQLVSTVK .......A..............SD..M..............................S... xenTro tetNig SDESSEEASFYEILPCCARFRCGEAIAEGQWHHLVLVMSKGMLKNSMATLYIDGQLINTVK S.ES...A................A.A...................M.T.......I.... tetNig takRub SDESSEEASFYEILPCCARFRCGEVIAEGQWHHLVLVMSKGMLKNSMATLYLDGQLINTVK S.ES...A................V.A...................M.T..L....I.... takRub gasAcu SDDSREDSFFYEILPCCARFRCGELIAEGQWQHLVLVMSKGMLKNSMATLYLDGQLVNTVK S..SR.D.F.................A....Q..............M.T..L......... gasAcu oryLap SDESSEEASFYEILPCCARFRCADLIAEGQWHHLVLVMSKGMLKNSMATLYIDGQLVNTVK S.ES...A..............AD..A...................M.T............ oryLap danRer VDDFSEESSFYEILPCCARFRCADLITEGQWHHLLLVMSKGMLKNSMATLYIDGQMVSTVK ......................AD..T.......L...........M.T......M.S... danRer calMil VDDFSEESSFYEILPCCARFRCTDLINEGQWHHLVLVMSKGMLKNSTATLYVDGQHVNTVK ......................TD..N.....................T..V...H..... calMil * * Less conseervation of this position in paralog WDFY4: WDFY4_hg18_18 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK WDFY4_panTro2 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK WDFY4_gorGor1 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK WDFY4_ponAbe2 DVMEPEDDSEPSAGCQLQVRCGQ L LTCGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK WDFY4_rheMac2 DVMEPEDDSEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLDGQVIGSAK WDFY4_calJac1 DVMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRHCTVSTCLNGQVIGSAK WDFY4_micMur1 DVMEPEDDSEPSGGRQLLVRWSQ L LTWGQGHHLGGVVTKEMKRHCTISTYLDGQGIGSAK WDFY4_otoGar1 -IMEPEDDSEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGSAK WDFY4_tupBel1 -VMEPEDDAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEVKRSCTVSTYLDGQGIGSAK WDFY4_mm9_18_ DAMEPEDEAEPSAGRQLQVRCSQ L LTCGQWYHLAVVVSKEMKRNCSVTTYLDGQAIGSAK WDFY4_rn4_18_ DIMEPEDEAEPSAGRQLQVRCSQ L LACGQWYHLAVVVSKEMKRNCTVTMYLDGQAIGSAK WDFY4_dipOrd1 DIMEPEDEGEPSAGRQLQVRCGQ H LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGLAK WDFY4_cavPor3 DFMEPEDTIEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGSAK WDFY4_speTri1 DIMEPEDESEPSAGCQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCIISTYLDGQVIGSAK WDFY4_oryCun1 DVMEPEDDAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQLTGSAK WDFY4_ochPri2 DVMEPEDDAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQLTGSAK WDFY4_turTru1 DVMEPEGDPEPSAGRQLRVRCGQ M LACAQWHHLAVVVTKEMKRNCTVSTYLDGQVVGSAK WDFY4_bosTau4 DVMELEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVFTYLDGQVIGSAK WDFY4_equCab2 DIMEPEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAVGSAK WDFY4_felCat3 DIMEPEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQIVGSAK WDFY4_canFam2 DVMEPEDDPEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQITGSAK WDFY4_myoLuc1 DVMEPEDNAEPSAGRQLQVRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQAIGSAK WDFY4_pteVam1 DVMEPEDDSEPSAGRQLRVRCGQ L LACGQWHHLAVVVTKEMKKNCTVSTYLDGQVIGSAK WDFY4_sorAra1 DVMEPEEDFEPSAGRQLRVRCGQ L LTCGQWHHLTVVVTKEMKRNCTISAYLDGQVIGSAK WDFY4_loxAfr2 -AMEPEDVAEPSAGRQLQIRCGQ L LACGQWHHLAVVVTKEMKRNCTVSTYLDGQIIGSAK WDFY4_proCap1 DTMEPEDVAEPSAGCQLQVRCGQ L LACGQWHHLAVVVNKEMKRNCTVSTYLDGQIIGSAK WDFY4_echTel1 DAMEPEGDAEPSAGCQLQVKCGQ L LACGQWHHLAVVITKEMKRNCIVSTYLDGQIIGSAK WDFY4_dasNov2 -AMEPEDAAEPSAGCQLQVRCGQ Q LTCGKWYHLVVVVTKEMKRNCTVSTYLDGQIIGSAK WDFY4_choHof1 DVMEPEDDTEPSAGRQLQVRCGQ L LACGQWYHLVVVVTKEMKRNC-ISTYLDGQLIGSAK WDFY4_monDom4 DVMEPEDIHEPSAGSRLQFHCGN L LSSGQWHHLAVVVSKEMKRNCAVSTYINGQLIGSAK WDFY4_ornAna1 DIMEPEETSEPPAGSRVQFKCVK L ITTGQWHHLAIVVAKEMKRTCVVRAFIDGQLVGSAK WDFY4_galGal3 DIMEPEGEVQPFPE-QVQFGCGK L LVTGQWHHLTVTVAKEAKKNCTVSAFINGQMLGSAK WDFY4_taeGut1 DIMEPEGEVLPFPG-QVKFGCGK L LVTGQWHHLTVTVAKEAKKSCIVAAYINGQMLGSAK WDFY4_tetNig1 DIMEAEVYSDITA-R-LRFRCSS M LIPGQWHHLVVVMTKDVKKSCVTSVYFNGKAFGSGK WDFY4_fr2_18_ NIMEPEVHSYITP-R-LRFRCSN M LVPGQWHHLAVVMSKDVKKSCVTSVYFNGKAFGSRK WDFY4_gasAcu1 DMMEPEVLPHPFD-R-LRFQCSS M LVPGQWHHLAVVLSKDVKKSCIASAYFNGKAVGTGK
Case of XYLT1
chr6_2360 XYLT1 5 61 D=3(110) A=5(107) >contig00001 length=488 numreads=10 RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPI ....L........................................................D..... ^
This non-conservative change A-->D is backed by three Sarcophilus reads. However all three are fairly near the end of a minus strand read so none cover the whole exon (raising mild concerns over read quality, given the unusual c-->a base transversion), yet none are long enough to span the next intron to reach the short following exon (leaving some mild pseudogene and paralog issues). Although blastn of extended opossum dna shows that the expected downstream phase 2 splice donor is present, that would also be expected in a close paralog or segmental duplication.
Pseudogene issues: None observed in any mammal using tblastn at wgs database. The detection technique here is a multi-exon query. Because the target database is genomic, recent processed pseudogenes actually give stronger matches because of longer contiguous matches, whereas ortholog matches are weakened by the attempt by blast to extend them. Hence processed pseudogenes surface at the top of match list.
Only a fragment of the gene can be recovered from current Sarcophilus reads, about 8 of 12 exons. However it cannot be determined without genomic assembly which exons 'belong' to the D containing exon, nor can the risk of including matches from the paralog be excluded. This gene has so-so conservation between human and opossum (270 myr roundtrip), 78% identity. which is somewhat puzzling in view of its enzymatic importance. However within marsupials conservation of most exons is in the mid-90's.
Paralog issues: XYLT2 (xylosyltransferase II) gives a moderate match but is not an issue in terms of accurately scoring tasmanian devil populations for the A-->D change. It does create problems in conserved exons in recovering full length genes in species where reads span only single exons. Note XYLT2 also has a conserved A at this position in all 34 available species back to lamprey, proving it an important invariant. Adjacent residues however are only moderately conserved.
XYLT1_homSap RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNAR RS+YLHR+V+++++ Y NVRVTPWRM TIWGGASLL+ YL+SMRDLLE+ W WDFFINLSA DYP RTN++LVAFLS+ RD NFLKSHGRDN+R XYLT2_homSap RSDYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYPTRTNEELVAFLSKNRDKNFLKSHGRDNSR
Homoplasy (recurrent mutation) issues: The sole homolog in Drosophila (CG17771, 41% identity) has been previously studied. Here the 424 residue is large and charged E in a motif SESD conserved within arthropods but not lophotrochozoa nor cnidarians such as Hydra magnipapillata (where the corresponding fragment has 63% identity) or Nematostella where A424 are A and G respectively. This is not the drosophila DxD motif however -- this occurs much later in the protein. A further very remote crystallographic paralog MGAT1 also has D here as discussed later.
XYLT1_homSap WRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDM R +TIWGGASLL+ LQ M DLL+ ++W WDF INLS +D+P++T D+LV FLS OXT_droMel KRFSTIWGGASLLTMLLQCMEDLLQ-SNWHWDFVINLSESDFPVKTLDKLVDFLSANPGR XYLT_hydMag WRMATIWGGASLLSMLLQMMEDTLKIKEWKWDFFINLSASDYPVQ XYLT_nemVec WSMATIWGGATLLQMLLKSMEDLIARKEWKWDFFINLSGNDFPIKVNT
Known variations: Not a known disease gene. Natural human polymorphisms in XYLT1 have been observed, P325R, P766A, V8391 and R892Q but these do not include changes near the locus under consideration here A424.
Structural significance: The region enveloping the key residue has a weak 30% match encompassing residues 328-535 (thus including the A-->D residue at 424) that nonetheless is adequate for structural modeling to PDB structure 2GAK -- our residue is part of a short type I beta turn connecting strands 4 and 4' of the donor Rossmann domain. The determined structure match to residues 86-289 is a somewhat similar enzyme, 6-N-Acetylglucosaminyltransferase, a product of the GCNT1 gene. A glycine has replaced the alanine, showing the latter is not a deep invariant critical to this class of enzyme.
This region has been compared in the structural overlay below to yet another glycosylating enzyme, rabbit MGAT1 beta-1,2-N-acetylglucosaminyltransferase. In this enzyme, this short beta turn carries the critical DxD motif that provides bound Mn++ for the UDP of incoming substrate. Comparing GCNT1 and XYLT1 aligns CGMD to SAAD (SDAD in Sarcophilus) in XYLT1. These residues are EDDL (DxD motif) in MGAT1. In other words, A424D of Sarcophilus is in fact physically realizable by D in functional MGAT1. This middle D is invariant throughout vertebrate MGAT1 even as the 'x' residue. However XYLT1 and MGAT1 have no significant alignment at the amino acid level and A-->D (or any other residue) is never observed in XYLT1.
The size of XYLT1 presents an unresolved mystery requiring a crystallographic determination. A simply glycosylation reaction could be accomplished in a bacterium with perhaps 250 residues, yet here the enzyme is 959 residues long, almost 4x the minimum even allowing for targeting peptides and a transmembrane segment.
A second puzzling aspect of glycosylases generally is their lack of homology -- 91 families exist of which only 29 have determined representatives (as tracked at the CAZy database. XYLT1 and XYLT2 are typical in belonging to a small isolated glycosyltransferase family 14 sharing no real sequence homology with other glycosylases (other than the DxD divalent cation coordination motif which could have arisen convergently). Structurally, known glycosylase folds are classified as GT-A (DxD plus single Rossmann-like UDP-binding fold) or GT-B (double).
Note the immediately preceding residues NLS constitute a potential glycosylation site, plausibly realized given the localization of the enzyme (Golgi or extracellular matrix) yet completely consistency with the beta role is required. NLS is invariantly conserved in both XYLT1 (even in drosophila and cnidaria) and XYLT2. While adjacent residues are not normally considered relevant to the NxT/S motif, potentially the substitution of D could interfer with this post-translational modification, were it to occur. This would require the glycosylated serine would be at the surface of the protein, contrary to the best PDB fit. Clearly a large attached carbohydrate would block interactions of immediately adjacent residues.
Functional significance: The protein has been the subject of about a dozen publications. Xylosyltransferases I and II are the chain-initiating enzymes in the biosynthesis of glycosaminoglycans. XYLT1 is the initial and rate-limiting enzyme, transfering UDP-xylose to specific serine residues of a target protein. It is localized to the endoplasmic reticulum and Golgi apparatus as a single-pass membrane protein, but with some fraction also secreted to the extracellular space. The domain match is pfam02485, defined as 'core-2/I-branching' reflecting the branch the added carbohydrate introduces to the growing chain in chondroitin and heparan sulfate and post-translational proteoglycan production. The precise function of XYLT2 has not been established.
Some 19 residues have been subject to experimental mutation though none of the glycosylation sites. Only 8 of the 19 induced mutations affected enzymatic activity (yet without lowering UDP=xylose binding), even though the comparative genomics at bottom shows all 19 sites are equally invariant back to lamprey. Thus residues can be under tremendous selection for a variety of reasons other than substrate binding or direct or indirect role in catalysis.
It is known that formation of abdominal aortic aneurysms can be caused by a destructive remodeling of the extracellular matrix in the vascular wall -- A115S enhances this risk. This bears no apparent relation to the A424D allele (human numbering) in tasmanian devil. The 745DWD747 motif has been shown essential to catalytic activity but again lacks immediate relevence. Reduced XYLT1 activity is a known contributor to male sterility. XYLT1 is elevated in connective tissue diseases such as systemic sclerosis, osteoarthritis, and pseudoxanthoma elasticum.
The connection to tumors or cancers is tenuous. GCNT1 expression is highly correlated with tumor progression in a number of cancers. It is overexpressed in colorectal, lung, and prostate cancer. It is a very weak paralog. Similarly the proteoglycans produced by XYLT1 are important regulators in extracellular matrix deposition, cell membrane signal transfer, morphogenesis, cell migration, normal and tumor cell growth. Mouse knockouts of XYLT2 produce polycystic liver and kidney disease.
In summary, this putative change in tasmanian devil could use additional sequences validation. While not likely linked to facial tumors, the A-->D allele is very undesirable in an inbreed population in view of its role in aortic aneurisms and male sterility. The several billion years of branch length invariance of the alanine argues for no tolerance for variation at this position.
exon 5 ^ exon 6 homSap RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR panTro2 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR gorGor1 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSXGRDNA- ponAbe2 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR rheMac2 RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR calJac1 RSNYLHRQVLQFSRQYGNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR tarSyr1 RSNYLHRQVLQFARQYDNIRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNA- otoGar1 RSNYLHRQVLQFARQYGNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR --------------------------- tupBel1 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR mm9_5_1 RSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR rn4_5_1 RSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR dipOrd1 RSNYLHRQVLQFATQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLQMPDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR cavPor3 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMQDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR speTri1 RSNYLHRQVLQFAGQYGNVRVTPWRMATIWGGA SLLATYLQSMRDLLEMTDWPWDFFINLSAADYPIR --------------------------- ochPri2 RSNYLHRQVLQMARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMPDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR vicPac1 rSDYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR --------------------------- bosTau4 rSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR equCab2 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR felCat3 RSNYLHRQVLQFARQYDNVRVTPWRMATIWGGA SLLSTYLQGMRDLLEMTDWPWDFFINLSAADYPIR --------------------------- canFam2 RSNYLHRQVLQFARQYGNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR myoLuc1 RSNYLHRQVLQFARQYSNVRVTPWRMATIWGGA SLLATYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR pteVam1 RSNYLHRQVVQVARQYDNVRVTPWRRATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR --------------------------- loxAfr2 RSNYLHRQVLZFARQYANVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR proCap1 RSNYLHRQVLQLARQYPNVRVTPWRMATIWGGA SLLSTYLQSMRDLLEMTSWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR echTel1 RSNYLHRQVLQFTGQYDNVRVTPWRMATIWGGA SLLTTYLQSMRDLLEMADWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR dasNov2 RSNYLHRQVLQFARQYANVRITPWRMATIWGGA SLLSTYLQSMRDLLEMSDWPWDFFINLSAADYPIR --------------------------- monDom4 RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR macEug RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR sacHar1 rSNYLHRQVLQFAGQYQNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIr TNDQLVAFLSRYRDMNFLKSHGRDNAR sacHar2 SLLSTYLQSMRDLMEMTDWPWDFFINLSDADYPIr TNDQLVAFLSRYRDMNFLKSHGRDNAR ornAna1 RSNYLYRQVLQFAGQYPNVRVTSWRMATIWGGA SLLTTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYREMNFLKSHGRDNAR galGal3 RSNYLHRQVLQFANQYPNVRVTSWRMATIWGGA SLLSTYLQSMRDLMEMNDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR taeGut1 RSNYLHRQVLQFASQYPNVRVTSWRMATIWGGA SLLTTYLQTMKDLMEMSDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR xenTro2 RSHYLHRQVLQFASQYPNVRVTSWRMSTIWGGA SLLSTYLQSMRDLLEMSDWSWDFFINLSAADYPVR --------------------------- tetNig1 RSNYLHRQVQALAALYPNVRVTPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR --------------------------- fr2_5_1 RSNYLHRQVQALAALYPNVRVTPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR TNDQLVAFLSKYRNMNFIKSHGRDNAR gasAcu1 RSNYLHRQVLSLAAQYSNVRATPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR --------------------------- oryLat2 -SNYLHRQVQIMAMKYPNVRVTPWRMATIWGGA SLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR --------------------------- danRer5 RSNYLHRQMVALAHQYPNVRVTSWRMSTIWGGA SLLTMYLQSMKDLLAMRDWSWDFFINLSAADYPIR --------------------------- squAca1 RSNYLHREAMQLAQRYSNIRITPWRMVTIWGGA SLLKMYLHCMKDLLEMTDWQWDYFINLSATDYPTR TNDELMGFLSKYRGKNFLKSHGRDNAR leuEri1 RSNYLHREVMQLAQQYPNVRVTPWRMVTIWGGA SLLKMYLNCMKDLLEMTDWHWDYFINLSATDYPTR TNDELVGFLSRYREKNFLKISR----- petMar1 RSNYLQRQVLQVAERYPNVRVTPWRMATIWGGA SLLTMYLRTMKDLLDMADWAWDFFINLSATDYPIR TNDQLVAFLTKYRDKNFLKSHGRDNNR
The A is also conserved in the paralog XYLT2: ^ XYLT2_hg18_4_ RSDYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_gorGor1 RSNYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_ponAbe2 RSNYLHREVVELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_rheMac2 RSDYLHREVVELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_calJac1 RSNYLHREVAELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_tupBel1 RSNYLHREVVELAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_mm9_4_1 RSNYLYREVVELAQHYENVRVTPWRMVTIWGGASLLRMYLRSMKDLLEIPGWTWDFFINLSATDYPTR XYLT2_rn4_4_1 RSNYLYREVVELAQHYDNVRVTPWRMVTIWGGASLLRMYLRSMKDLLEIPGWTWDFFINLSATDYPTR XYLT2_dipOrd1 RSDYLHREVVELAKQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR XYLT2_cavPor3 RSNYLHREVVALAQRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_speTri1 RSNYLHREVVELAQRYENVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_ochPri2 ---YLHREVVELAQQYENVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWTWDFFINLSATDYPTR XYLT2_turTru1 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPDWAWDFFINLSATDYPTR XYLT2_bosTau4 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR XYLT2_equCab2 RSNYLHREVVELARQYDNVQVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR XYLT2_felCat3 RSNYLHREVVELARRYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_canFam2 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR XYLT2_myoLuc1 RSNYLHREVVELARQYDNIRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR XYLT2_pteVam1 RSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_eriEur1 RSNYLHREVVELARHYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTR XYLT2_proCap1 RSNYLHREVVELARQYDNMRVTPWRMVTIWGGASLLRMYLRSMRDLLEVPGWAWDFFINLSATDYPTR XYLT2_monDom4 RSNYLHREVVALAQHYANVRVTPWRMGTIWGGASLLKMYLRSMQDLLEAPGWTWDFFINLSATDYPTR XYLT2_macEug RSNYLHREVVALAQHYANVRVTPWRMGTIWGGASLLKMYLRSMQDLLEAPGWTWDFFINLSATDYPTR XYLT2_sarHar RSNYLHREVVALAQHYANVRVTPWRMGTIWGGASLLKMYLRSMQDLLEAPGWTWDFFINLSATDYPTR XYLT2_galGal3 RSNYLHREAVELAQHYPNIRVTPWRMVTIWGGASLLKMYLRSMKDLLELTEWPWDFFINLSATDYPTR XYLT2_taeGut1 RSSYLHREAVELARHYPNIRVTPWRMVTIWGGASLLKMYLRSMKDLLELSEWPWDFFINLSATDYPTR XYLT2_anoCar1 RSTYLHREVVEMAQHYPNIRVTPWRMVTIWGGASLLKMYLHSMKDLLEMTDWTWDYYINLSATDYPTR XYLT2_xenTro2 RSNYLHREVVRLAQSYENMRVTPWRMVTIWGGASLLTMYLRSMKDLLEVPDWPWDFFINLSATDYPTR XYLT2_tetNig1 RSGYMHREVLQVAQQYPNIRATPWRMVTIWGGASLLKAYLHSMQDLLSMLDWKWDFFINLSATDFPTR XYLT2_fr2_4_1 RSNYLHRQVQALAALYPNVRVTPWRMATIWGGASLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR XYLT2_gasAcu1 RSNYLHRQVLSLAAQYSNVRATPWRMATIWGGASLLTMYLRSMADLLAMRDWSWDFFINLSAADYPIR XYLT2_oryLat2 RCSYMHREVLQMAKHYPNIRATPWRMVTIWGGASLLKAYLRSMQDLLSMAEWKWDFFINLSATDFPTR XYLT2_danRer5 RSNYLHRQMVALAHQYPNVRVTSWRMSTIWGGASLLTMYLQSMKDLLAMRDWSWDFFINLSAADYPIR XYLT2_petMar1 RSNYLQRQVLQVAERYPNVRVTPWRMATIWGGASLLTMYLRTMKDLLDMADWAWDFFINLSATDYPIR
The comparative genomics of the 19 XYLT1_homSap residues replaced by experimental mutagenesis. The key residue columns have been sliced out of intact protein accompanied by a few residues of flanking context and then concatenated to make a compact display (dots used if identical to human).
C257A none C542A none D745G enz- C276A enz- C561A enz- D745E none C285A none C563A none W746DNG enz- C301A none C572A enz- D747GE enz- D314G none C574A enz- C920A none D316G none C675A none C927A none C471A enz- C933A none * * * * * * * * * *** * * * homSap CDISGKEAISALSRAKSKHCRQEIGETYCRHKLGLLMPEKVTRFCPLEDEDECDCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSSCRVGTDWDAKERDICATGPTACPVMQTCSQ panTro2 ....................................................................................................................... gorGor1 .....................................................................X................................................. ponAbe2 ....................................................................................................................... rheMac2 ...............................................................................................................R...I... calJac1 ....................................................................................................................... tarSyr1 ................................H...................................I.................................V.....S.......... micMur1 ........V.................................P....D....................................................................... otoGar1 ........................................................................................................T.............. tupBel1 .E............S........................................H................................................T.............. musMus1 ............T...........A................A..............................................I.V.............T.............. ratNor ............T...........A................A............................................................V................ dipOrd1 ........................A.........Q..................................................................EV.SV.LS...T.PA... cavPor3 ..............S..............................................................................................S......... speTri1 ..............S..R...........Q.........RL..L..........................................................V................ oryCun1 .E............S.......Q................R..............................................................V.S..........S..H ochPri2 .E.....................................R................................................................T...S.......... turTru1 ..............S.............................................................................................S.......... bosTau4 ..............S............................L.................................................................V......... equCab2 ..............S.................................................................................E...........S.......... felCat3 ......................................K................................................................................ canFam2 .........................D........M...K......S..........................................................T.............. myoLuc1 ..............S......................................................................................................TH pteVam1 ..............S...........-............................................................................................ proCap1 ..............S.........V................A................................................L...........-GGA.AR...MLPAG.. echTel1 ..............S.........A.A..S....R......A.L............................................................S..........A... dasNov2 ..............S.........A..................L...........G...................................I..E.......V.T........L.-... monDom4 ..............S...Q.....A.I..Q..V.K.....................S...............................R..I..E.........T..........A... ornAna1 ..............S...Q.....A....Q..Y.K.....................S..................................I..E........................ galGal3 .EVT.......M......P.....ADV..Q..H.K..........T.............................................I..E..........I............. taeGut1 .EVT.......M.....QQ.....ADV..Q....K....Q.....A.........ES...............................T.....E..........A...S.....A... anoCar1 .E......L.........P.....A......RQ.K....Q...L...Q.D.....ES..................................I.AE.........S..........A.T. xenTro2 .E.T..............Q.....A.V..Q..Q.K........L...........ES..................................I.A........V.SV.......L.G.A. tetNig1 .E..........A.....E...Q.A.V.....E.Q....R...Y...........ES..................................I..E..P......T...SS.....S.A. fr2_3_1 .E..........A.....E...Q.A.VF....E.Q........Y...........ES.....................................E..........V.........A.PK gasAcu1 .E............V...E...Q.A.V.....E.Q....T...Y......H....GSL.................................I.............V.........A.PK oryLat2 .E................D...Q.A.V....RE.R........Y....E......GSL..............................A..IS....P....V..V.........A.PK danRer5 .E..............T.E...Q.V.V.....EHQ........Y..V...V....GSL..............................A..IS....P....V..V...S.....A.AK petMar1 .E.A....L......R.AQ.K...ADVV.L.QE.K....SLP....I...V....GSL..............................A..IS....P....V.S...SG.......RE
>XYLT1_homSap MVAAPCARRLARRSHSALLAALTVLLLQTLVVWNFSSLDSGAGERRGGAAVGGGEQPPPAPAPRRERRDLPAEPAAARGGGGGGGGGGGGRGPQARARGGGPGEPRGQQPASRGALPARAL DPHPSPLITLETQ DGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPPSRQKELLKRKLEQQEKGKGHTFPGKGPGEVLPPGDRAAANSSHGKDVSRPPHARKTGGSSPETKYDQPPKCDISGKEAISALSRAKSKHCRQEIGETYCRHKLGLLMPEKVTRFCPLE GKANKNVQWDEDSVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDK RSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR FIRKQGLDRLFLECDAHMWRLGDRRIPEGIAVDGGSDWFLLNRRFVEYVTFSTDDLVTKMKQFYSYTLLPAE SFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQ QTARPTFFARKFEAVVNQEIIGQLDYYLYGNYPAGTPGLRSYWENVYDEPDGIHSLSDVTLTLYHSFARLGLRRAETSLHTDGENSCR YYPMGHPASVHLYFLADRFQGFLIKHHATNLAVSKLETLETWVMPKKVFKIASPPSDFGRLQFSE VGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSNRQPIKP EEALKLHNGPLRNAYMEQSFQSLNPVLSLPINPAQVEQARRNAASTGTALEGWLDSLVGGMWTAMDICATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR* >XYLT1_monDom MVAALCARRLARRSHSALIAALTVLLLQTLIVWNFSSLDSGAGDHRGGAAAGGPPPAPRRERRDLPLEPAAAGEGERGPAGGQLLRERGGGHGEHRAQHPPRRGGLPGRAL EPPPSPFTSLETQ DGYFSHRPKEKMRTDSNNENSVPKDFENIDNSNFAPRTQRQKHQPDLGKKPLSKQKEHLKKKLEQDEKVKENSLLGKGSNEALQYSNQAAQNSSQGKKSSRLPHSRKNGAGSPELKYDQPPRCDISGKEAISALSRSKSKQCRQEIAEIYCQHKVGKLMPEKVTRFCPLE GKANNNVRWDEDSVEYMPANPVRIVFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDK RSNYMHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR FIRKQGLDRLFLECDTHMWRLGDRKIPEGITVDGGSDWFLLNRKFVEYVTFSNDDLVTKMKQFYSYTLLPAE SFFHTVLENSPHCGTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPADFHRFQ QTARPTFFARKFEAVVNQEIIGQLDYYLYGNYPSGTPGLRSYWENVYDEPDGIHSISDVVLTMYHSFTRLGLRRAETSLHTDGENSCR YYPMGHPVSVHLYFLADHFQGFLIKHHATNLAVSKLETLETWVMPKKVFKIANPPSDFGRLQFSE IGTEWDAKERIFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNIIAATYDILIESSAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVTPLTFSNKQPIKP DESLKLHNGPPRNAYMEQSFQGLNPVLNIPINLAHVEQARRNAATTGAKLESWVDSLVGGIWSAVDICAIGPTACPVMQTCSQTSWSSLSPDPKSELGAIKPDGRLR* >XYLT1_macEug fragment MVAALCARRLARRSHSALIAALTVLLLQTLIVWNFSSLDSGAGDHRGGEQHAGGEPPPAPRRERRDLAPESRAAAGEEGGGGGRGPQPRGYKLPLERGGGGGGGHREHRPQQTPRRGGPAAGAAQLPGQAL ... DGYFSHRPKEKMRTDSNNENSVPKDFENIDNSNFAPRTQRQKHQPDLGKKSLSKQKEQLKKKLEQEEKAKENSLLGKSSNEAMQYSNQAAQNSSAAKASPKSSKQPHTRKNGAGSPELKYDQLPRCDISGKEAISALSRSKSKQCRQEIAEIYCQHKVGKLMPEKVTRFCSLE GKANNNVRWDEDSVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDK RSNYLHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR ... FIRKQGLDRLFLECDTHMWRLGDRKIPEGITVDGGSDWFLLNRKFVEYVTFSNDDLVTKMK... SFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPADFHRFQ ... YYPMGHPVSVHLYFLADRFQGFLIKHHATNLAVSKLETLETWVMPKKVFKIANPPSDFGRLQFSE IGTDWDAKERIFRNFGGLLGPKDEPVGMQKWGKGP... DESLKLHGGPPHNAYMEQSFQGLNPVLNIPINLAHVEQARRNAATTGPKLESWVDSLVGGVWSAMDICAIGPTACPVMQTCSQTSWSSLSPDPKSELGAVKPDGRLR* >XYLT1_sarHar fragment missing 5-6 exons ... ... DGYFSHRPKEKMRTDSNNENSVPKDFENIDNSNFAPRTQRQKHQPDLG...PHVRKNGVGSPELKYDQPPRCDISGKEAISALSRSKSKQCRQEIAEIYCQHKVGKLMPEKVTRFCPL. rSNYLHRQVLQFAGQYQNVRVTSWRMATIWGGASLLSTYLQSMRDLMEMTDWPWDFFINLSAADYPIR TNDQLVAFLSRYRDMNFLKSHGRDNAR FIKKQGLDRLFHECDSHMWRLGERQIPEGIVVDGGSDWFALTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAE SFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPADFHRFQ ... YYPMGHPVSVHLYFLADRFQGFLIKHHATNLAVS... IGTDWDAKERIFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESSAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVTPLTFSNRQPIKP DESLKLHNGPPRNAYMEQSFQGLNPVLNIPINLAHVEQARRNAAITGPKLENWVDSLVGGIWSAVDICAIGPTACPVMQTCSQTSWSSLSPDPKSELGAIKPDGRLR*
Case of ATP4A
chr4_18550 ATP4A 6 16 C=4(130) R=3(74) >contig00001 length=906 numreads=10 TAQGLVVNTGDRTIIGRIASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFIVAMCIGYTFLRAMVFFMAIVVAYVPEGLLATVT ................C........................................................................ ^
Case of VPS72
chr2_30280 VPS72 5 15 R=3(59) K=2(51) >contig00001 length=591 numreads=6 NYERLEADKKKQVHKKRKCPGPVITYHSMTVPLLAEPGPKEENVDVE ...............R..................T............ ^
Case of ABCC1
chr6_5144 ABCC1 23 4 Q=2(69) P=2(80) looks like a frame-shift problem in monDom5 >contig00001 length=802 numreads=10 HLCFPRLHLDLLHNVLRSPMSFFERTPSGNLVNRFSKEMDTVDSMIPQIIKMFMGSLFNVIGACIIILLATPIAAIIIPPLGLIYFFVQ ....Q.................................................................................... ^
Discarded candidates
Below are three initial candidates that had to be discarded without detailed followup. One arose from repeated frameshifts in the critical region, another exhibited homoplasy with marsupials, and the third too extensive of an accepted reduced alphabet at the site. Thus while these three genes do not meeet the search criteria, they are nonetheless instructive in illustrating those criteria and making clear these are quite restrictive.
Case of ACOT12
chr3_5872 ACOT12 14 14 I=3(95) V=3(110) 'wobbly' >contig00001 length=472 numreads=6 NTYVVAVKSVTLASIPPSPQYNRSEITCAGFLIRAVDSNSCT .................................Q....S... ^
Here an I-->V change is seen in some tasmanian devils reads relative to opossum and wallaby. Here V is more typical of a theran mammal. Note I is also seen in armadillo, a placental, and A occurs in platypus and various other mammals. ACOT12, a acyl-CoA thioesterase, does not track back well in earlier diverging species. Because of the observed homoplasy, this locus is an unsuitable example of a significant amino acid change in Sarcophilus. However it illuminates the nature of suitable candidates and so is retained here.
^ ACOT12_hg18_14 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI ACOT12_panTro2 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI ACOT12_gorGor1 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI ACOT12_ponAbe2 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSNSCI ACOT12_rheMac2 NTYTVAVKSVILPS V PPSPQYIRSEIICAGFLIHAIDSSSCI ACOT12_calJac1 NTYTVAVKSVMLPS V PPSPQYIRSEIICAGFLIHAIDSNSCI ACOT12_micMur1 NTYTVAVKSVILPS V PPSPQHVRSEIICAGFLIHAADSNSCT ACOT12_otoGar1 NTYMVAAKSVILPS V PPSPQYIRSEIICAGFLIHTIDSTSCT ACOT12_tupBel1 NTYTVAVKSVTLPS V PPSPQYIRSDIICAGFLIRPVDSSSCT ACOT12_mm9_14_ NTYTVALRSVVLPS V PSSPQYIRSEVICAGFLIQAVDSNSCT ACOT12_rn4_14_ NTYIVALMSVVLPS V PPSPQYIRSQVICAGFLIQPVDSSSCT ACOT12_dipOrd1 NTYVVATKSVILPS V PPSPAYIRSEAVCSGFLIKAVDSSSCT ACOT12_cavPor3 DTYLVAVKSVVLPA V PPSPGYTRSEVALAGFLIQPTDHSSCT ACOT12_oryCun1 HAYTVAAKSVMLPS A PPSPDHTRSEIICAGFLIHAIDSHSCT ACOT12_ochPri2 HAYVVAVKSVVLPS A PPSPEYIRGEIVCAGFLIHAIDSHACT ACOT12_vicPac1 NTYTVAVKSVILPS V PPSPQYVRSEITCAGFLIHAIDNSSCT ACOT12_turTru1 HTYTVAVRSVILAS V PPSPQYSRSEIISAGFLIRAIDSSSCT ACOT12_bosTau4 HTYVVAVRSVILPS V PPSPQYVRSEIECAGFLIHATDSSSCT ACOT12_equCab2 KTFSVAAKSVILPS V PPSPQYMRSEIRCAGFLICAIDNSSCT ACOT12_felCat3 STYTVAVKSVLLPS V PPCPHYIRSEIICAGFLIRAIDSSSCT ACOT12_canFam2 NTYTVAVKSVTLPS V PPSPQYSRSEILCAGFLIHAIDSSSCT ACOT12_myoLuc1 NTYTVAVKSVILPS V PPSPQYVRSEIICAGFLIHAIDSSSCT ACOT12_pteVam1 NTYTVAVKSVILPS V PPSPZYVRSEIVCAGFLIHAIDGSLCI ACOT12_eriEur1 STFTVAMKSVLLAS V PSSPQYIRSEITCAGFVIHAVSSNSCI ACOT12_sorAra1 NAFTVAVKSVILPS V PPSPQYMRSEIICAGFLIHATDSNSCI ACOT12_loxAfr2 D--TVAVKSVLLPS V PPCPQYIRSEIIRAGFLIHTIDSNSCT ACOT12_echTel1 TTYTVALRSVLLPS V PSSPNYVRGEIICAGFLVHPIDSSACT ACOT12_dasNov2 NTYTVAVKSVVLPS I PPSPQYIRSEIICAGFLIHAIDSSSCT ACOT12_choHof1 NSYTVAAKSVVLPS V PPSPQYIRSETICAGFLINAIDSSSCT ACOT12_monDom4 NTYVVAVKSVTLAS I PPSPQYNRSEITCAGFLIRAVDSNSCT ACOT12_macEug NTYVVAMKSVTLAS I PPSPQYNRSEITSAGFLIQAVDSNSCT ACOT12_sacHar1 NTYVVAVKSVTLAS I PPSPQYNRSEITCAGFLIQAVDSSSCT ACOT12_sacHar2 NTYVVAVKSVTLAS V PPSPQYNRSEITCAGFLIQAVDSSSCT ACOT12_ornAna1 DSYLVAVKSVILAS A PPSHQYIRSEIPCAGFLVEALDSSSCK
Case of FLI1
chr4_11174 FLI1 3 32 N=2(63) K=3(47) >contig00001 length=575 numreads=9 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEKNGPPPNMTTNERRVIVPA .................................................. ^
Here the N-->K change is a non-conservative substitution in the sense asperagine is merely polar whereas lysine is bulkier and negatively charged. The N is highly invariant at this position back to teleost fish. FLI1 is a transcription factor associated with a leukemia virus integration site and Ewing sarcoma.
This would be a promising candidate except for the fact that the three reads establishing K clearly are plagued by frameshifts at the critical region. Possibly anomalous base composition is responsible here (ggatgagaagaacggcccccctcc) -- which is no doubt giving rising to transcriptional slippage generating homoplasic deletions of polyP -- or perhaps low coverage. This change is unlikely to be validated upon additional bulk or targeted sequencing because these lack motivating evidence.
>FP1JAYN01BA7O5 and FP5M7SR01ERAQP Frame = +1 Frame = +2 Query: 1 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEKNGPP 36 KNGPPPNMTTNERRVIVPA 50 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK P KNGPPPNMTTNERRVIVPA Sbjct: 37 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEKKRSP 144 KNGPPPNMTTNERRVIVPA 187 >FP1JAYN01DX0A1 length=254 Query: 1 ESPVDCSVNKCSKLVGGNESNPMN-YNTYMDEKNGPPPNMTTNERRVIVPA 50 ESPVDCSVNKCSKLVGGNESNPMN + + EK PPPNMTTNERRVIVPA Sbjct: 37 ESPVDCSVNKCSKLVGGNESNPMNLQHLHG*EKTVPPPNMTTNERRVIVPA 189 N P M N Y N T Y M D E K N G P P P N M T | | | | | | | | | | | | | | | | | | | | aatcctatgaattacaatacctacatggatgagaagaacggcccccctcctaacatgacc FLI1_hg18_3_ ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_panTro2 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_gorGor1 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_ponAbe2 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_rheMac2 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_calJac1 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_tarSyr1 ESPVDCSVSKCSKLVGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_micMur1 ESPVDCSVSKCGKLIGGGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_otoGar1 ESPVDCSVSKCSKLIGGGEANPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_mm9_3_9 ESPVDCSVSKCNKLVGGGEANPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_rn4_3_9 ESPVDCSVSKCNKLVGGGEANPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_dipOrd1 ESPVDCSVSKCSKLVGGGESNPMNYNSYIDEK N GPPPPNMTTNERRVIVPA FLI1_cavPor3 ESPVDCSVSKCSKLVGTGESNPMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_speTri1 ---VDCSVSKCSKLVFGGESNPMNYNSYLDEK N GPPPPNMTTNERRVIVPA FLI1_oryCun1 ESPVDCSISKCGKLVGGGEANAMSYNNYMDEK N GPPPPNMTTNERRVIVPA FLI1_vicPac1 ESPVDCSVSKCGKLVGGGESNTMSYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_turTru1 ESPVDCSVSKCGKLVGGGESNAMSYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_bosTau4 ESPVDCSVSKCGKLVGGGESNTMSYTSYVDEK N GPPPPNMTTNERRVIVPA FLI1_equCab2 ESPVDCSVSKCSKLVGGGESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_canFam2 ESPVDCSVSKCSKLVGGSESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_myoLuc1 ESPVDCSVSKCSKLVGGGESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_pteVam1 ESPVDCSVSKCSKLVGGGESNAMNYNSYIDEK N GPPPPNMTTNERRVIVPA FLI1_eriEur1 ESPVDCSVSKCSKLVGGGESNAMNYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_proCap1 ESPVDCSVSKCSKLAGGGESNPMNYNTYMDEK N GPPPPNMTTNERRVIVPA FLI1_dasNov2 ESPVDCSVSKYSKLVGGGESNPMTYSTYMDEK N GPPPPNMTTNERRVIVPA FLI1_choHof1 ESPVDCSVSKCSKLVGGGEATPMTYNTYMDEK N GPP-PNMTTNERRVIVPA FLI1_monDom4 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK N GPP-PNMTTNERRVIVPA FLI1_macEug ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK N GPP-PNMTTNERRVIVPA FLI1_sarHar1 ESPVDCSVNKCSKLVGGNESNPMNYNTYMDEK N GPP-PNMTTNERRVIVPA FLI1_ornAna1 ESPVDCSVSKCGKLVGSGESNPMNYNSYMEEK N GPPPPNMTTNERRVIVPA FLI1_galGal3 ESPVDCSVNKCSKLVGAGESNPMSYSTYMDEK N GPP-PNMTTNERRVIVPA FLI1_taeGut1 ESPVDCSMNKCGKLVGAGESNPMSYSTYMDEK N GPP-PNMTTNERRVIVPA FLI1_anoCar1 ESPVDCSVSKCNKLVPAGESNSLNYGTYMDEK N GPP-PNMTTNERRVIVPA FLI1_xenTro2 ESPVDCSISKCSKLIGGSENNAVTYNSYMDEK N GPPPPNMTTNERRVIVPA FLI1_tetNig1 ESPVDCSVGKCNKLVGGNDVSQMSYGSYMDEK N APP-PNMTTNERRVIVPA FLI1_fr2_3_9 ESPVDCSVGKCNKLVGGNDVSQMNYGSYMDEK N APP-PNMTTNERRVIVPA FLI1_gasAcu1 ESPVDCSVGKCNKLVGSNDTSQMNYGNYMDEK N APP-PNMTTNERRVIVPA FLI1_oryLat2 ESPVDCSVGKCNKLVGGNDTSQMTYGNYMDEK S APP-PNMTTNERRVIVPA FLI1_danRer5 ESPVDCSVGKCNKMVGGTEASQMNYTGYMDEK C APP-PNMTTNERRVIVPA
Csae of SPON1
chr5_8347 SPON1 11 20 V=3(65) I=2(66) wobbly >contig00001 length=433 numreads=5 GSTCTMSEWITWSPCSISCGVGMRSRERYVKQFPEDGSVCTVPTEETEKCTVNEEC ......................................I.N............... ^
Here two Sarcophilus reads show V-->I following residue 20 while three are V like opossum. It quickly emerges that wallaby also has I. Thus the change in tasmanian devil is within the normal reduced alphabet of this residue position. Various placentals show that T and M and even P are also accepted substituents here. Note too these are used clade-incoherently (eg primates alone are variable). Consequently this site is not under strong selection for V to begin with so SPON1 does not meet the selection criteria being used here.
^ SPON1_hg18_13 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_panTro2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_gorGor1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_ponAbe2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_rheMac2 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_calJac1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_tarSyr1 -GSTCTMSEWITWSPCCLSCV P GMRSREYYLK-FFEDGSVCSLTPKKTQNRTV-EZC SPON1_micMur1 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_otoGar1 DGSTCTMSEWITW-PCSISCG T GMRSRERYVKQFPEDVSVCTLPTEETEKCTVNEEC SPON1_tupBel1 EGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_mm9_13_ DGSTCTMSEWITWSPCSVSCG M GMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEEC SPON1_rn4_13_ DGSTCTMSEWITWSPCSVSCG M GMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEEC SPON1_dipOrd1 -GSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_cavPor3 DGSTCTMSEWIIWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_speTri1 EHSTCTMSEWITWSPCCISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_oryCun1 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_ochPri2 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_turTru1 DGSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPT-ETEKCTVNEEC SPON1_bosTau4 DGSTCTMSEWITWSPCSISCG T GTRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_equCab2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_canFam2 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_myoLuc1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_pteVam1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_eriEur1 DGSACTMSEWITWSPCSLSCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_sorAra1 DGSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_proCap1 -GSTCTMSEWITWSPCSISCG T GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_echTel1 ----CPMSEWITWSPRSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_dasNov2 -GSTCTMSEWITWSPCSISCG M GMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEEC SPON1_monDom4 DGSTCTMSEWITWSPCSISCG V GMRSRERYVKQFPEDGSVCTVPTEETEKCTVNEEC SPON1_macEug GSTCTMSEWMTWSPCSISCG I GMRSRERYVKQFPEDGSVCTVPTEETEK SPON1_sacHar1 GSTCTMSEWITWSPCSISCG V GMRSRERYVKQFPEDGSICNVPTEETEKCTVNEEC SPON1_sacHar2 GSTCTMSEWITWSPCSISCG I GMRSRERYVKQFPEDGSICNVPTEETEKCTVNEEC SPON1_ornAna1 DGSTCTMSEWITWSPCSVSCG M GMRSRERYVKQFPDDGSMCKVPTEETEKCVVNEDC SPON1_anoCar1 DGSTCMMSEWITWSPCSVSCG M GMRSRERYVKQFPDDGSMCKVPTEETEKCIVNEEC SPON1_xenTro2 EASTCMMSEWITWSPCSASCG M GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEEC SPON1_tetNig1 DASTCMMSEWITWSPCSASCG M GSRSRERYVKQFPDDGSICTLPTEETEDCVVNEEC SPON1_fr2_13_ DASTCMMSEWITWSPCSASCG M GSRSRERYVKQFPDDGSICTLPTEETEDCVVNEEC SPON1_gasAcu1 DASTCMLSEWITWSPCSLSCG M GTRSRERYVKQFPDDGSLCSLPTEETDNCVVNEEC SPON1_oryLat2 DGSTCMMSEWITWSPCSMSCG A GIRSRERYVKQFPDDGSICTLPTEETENCVVNEEC SPON1_danRer5 DSSTCMMSEWITWSPCSVSCG S GLRSRERYVKQFPDDGFACTHPTEETEPCTVNEEC
Other marsupial genes of interest
The collections below contain well-understood genes with very extensive comparative genomics. They can serve as a test bed for Sarcophilus assembly quality, a place where genuine anomalies or distinct adaptive features might surface (perhaps as phyloSNPs) and where marsupial phylogeny might be refined using rare genomic events in nuclear genes. The gene sets contain all available marsupial orthologs plus for context one flanking gene each from placentals and monotremes. These genes are available in much broader hand-curated sets elsewhere on this site.
Scattered new data is available for other marsupials and monotremes from 454 reads, Sanger trace data and transcripts:
Didelphis virginiana 88,207 traces 248 nuc Trichosurus vulpecula 169,115 traces 321 nuc 147,199 ests Sminthopsis crassicaudata 59 nuc 1,669 ests Sminthopsis macroura 3,411 traces 89 nuc Isoodon macrourus 6,144 traces, 70 nuc 1,319 ests Tachyglossus aculeatus 93,653 traces 243 nuc SRX000015 Baylor 454 sequencing of Monodelphis domestica genomic fragment library SRX000086 WUGSC 454 sequencing of Macropus eugenii genomic fragment library SRX000186 WUGSC 454 sequencing of Ornithorhynchus anatinus transcript SRX000122 WUGSC 454 sequencing of Tachyglossus aculeatus transcript SRX000121 WUGSC 454 sequencing of Tachyglossus aculeatus transcript
The running estimate of coverage of Sarcophilus genome combining all runs for 11 expected genes on different chromosomes:
59 of 68 exons found (87%) 3883 of 4339 amino acids available (89%)
Newbler has a bad tendency to create non-existent frameshifts as seen in these three reads for the same query gene:
Query: 82 ggtctctacggcagtgtcattgtcactggagggaacacactcttgcaagg |||||||||||||||||||||| |||||||||||||||||| |||||||| Sbjct: 167 ggtctctacggcagtgtcattg-cactggagggaacacactgttgcaagg FP1I63R01APY7E Query: 82 ggtctctacggcagtgt-cattgtcactggagggaacacactcttgcaagg ||||||||||||||||| |||||||||||||||||||||||| |||||||| Sbjct: 268 ggtctctacggcagtgttcattgtcactggagggaacacactgttgcaagg FKUJDAX01AWWZ3 Query: 82 ggtctctacggcagtgtcattgtcactggagggaac-acactcttgcaagg |||||||||||||||||||||||||||||||||||| ||||| |||||||| Sbjct: 268 ggtctctacggcagtgtcattgtcactggagggaacgacactgttgcaagg FKUJDAX01DZSZO
IRBP (96 marsupials)
Interphotoreceptor retinol-binding protein, poorly named by IGNC as RBP3 despite its complete lack of paralogs, is a 4 exon 1247 residue glycoprotein that shuttles retinoids between the photoreceptor cells and the retinal pigment epithelium. The protein's size results from four ancient internal tandem duplictions that became established prior to the intronation era.
The first three homology domains and part of the fourth are all encoded by the first large exon of 1090 amino acids. This exon has been much used in marsupial phylogeny (along with the first intron of transthyretin). Indeed the 96 marsupial species in 51 genera having determined IRBP sequences at GenBank include a Dec 2008 partial sequence for Thylacinus cynocephalus, as well as for Sarcophilus harrisii.
The closest matches to the thylacine IRBP are shown in the difference alignment of the first 60 residues below. These species all lie with the Dasyuromorphia. The indicated E-->K may be one of several phyloSNPs breaking this group into blue and green subclades.
The numbat Myrmecobius fits implausibly (its amino terminal sequence EF028750 needs verification) -- its affinities seem to lie with the Didelphimorphia. Thylacinus is not basal within Dasyuromorphia relative to Myrmecobius using IRBP. However this may be a case of mis-comparison of genes.
* * * STSKAPQHDSKFTNATQEELLALFQQIIKYQVLEGNVGYLRVDYIPGREMIEEVGEFLVN EU091365 0 Thylacinus cynocephalus .........P..A..................I............................ AY532676 3 Myoictis wallacei ........NP..A............................................... AY532687 3 Neophascogale lorentzii ........NP..A........T...................................... AY532686 4 Phascolosorex dorsalis .........P..V............................................... AY532670 2 Parantechinus apicalis ....V....P..A..................I.....................L...... AY532675 5 Myoictis melas .........P..A...................................D........... AY532679 3 Dasyurus hallucatus ...E.....P..A............K........D.............D........... AY532685 6 Sarcophilus harrisii ...E.......RA..........L............................Q..K.... EF028748 6 Sminthopsis crassicaudata .......R.P.LA.........SL.......................Q....Q....... EF028749 8 Planigale ingrami ..A......P.LA.V.....................................K....... EF028736 6 Antechinus stuartii ..A......P.L..V.....................................K....... EF028743 5 Micromurexia habbema ..A......P.LA.V.....................................K....... EF028744 6 Murexchinus melanurus ..A......P.L..V....V................................K....... EF028746 6 Paramurexia rothschildi ..A......P.LA.V.....................................K....... EF028747 6 Phascogale calura ..A......P.LA.V.....................................K....... EF028745 6 Phascomurexia naso .SA......P.LA.V.....................................K....... AY532667 7 Murexia longicaudata ......K..PNLA........T.L..R....................Q.VV.K....... EF028750 12 Myrmecobius fasciatus ..PET...VP..A.V........L..M....................Q.VV.K....... AY233765 13 Caluromys philander ..PET...VP.LA.V.......QL..M....................Q.VV.K....... AF257675 15 Caluromysiops irrupta ..PET...VP.LA.V......T.L..M....................Q.VV.K....... AF257688 15 Glironia venusta .IPET...VP..A.V.R....T.L..M....................Q.VV.K....... AF257683 16 Didelphis albiventris .IPE....VP.LA.I......T.L..M....................Q.VV.K....... AF257686 15 Gracilinanus microtarsus .IPET...VP..A.V......T.L..M....................Q.VV.K....... AF257676 15 Marmosops noctivagus .IPET...VP.LA.V........L..M....................Q.VV.K....... AY233788 15 Philander opossum .IPET...VP.LA.I......T.L..M....................Q.VV.K....... AF257689 16 Thylamys pallidior
Using Sarcophilus as probe in a different region, 721-900, we find this peculiar outcome: what appears to be a second very odd gene, XY difference, pseudogene, weird balanced polymorphism, nonhomologous recombination, sequence submission error, frameshifts, or systemic experimental error (eg Dasyurus maculatus AY532680 is identical to AY243439 outside the 15 amino acid block). However the genomic reads from individual Sarcophilus used in this project show no sign of this gene despite excellent coverage of the second type of gene.
Macropus and Monodelphis genomes only contain the second type of gene. All Didelphimorphia and Diprotodontia are of this type, as are platypus and all placentals. With the Sarcophilus genome, this can be resolved as it should have both and be the such first genome. Perhaps the alignment above is a mixture of type 1 and type 2 genes (resp. alleles). The Myrmecobius anomaly makes it more likely two distinct genes are present.
A definite pecularity seen in blast searches is the occurence earlier in the sequence of a very homologous segment for this very block, likely the homologous part of another of the internal tandem repeats. It is seen in both types of genes. Possibly internal non-homologus recombination or gene conversion has inserted first repeat sequence again in this distal block in place of what was relatively diverged sequence. Internal gene conversion would make IRBP extremely difficult to use in alignment-based phylogeny. As rare genomic event, it unites the species that have it but species that don't have it would have to be re-examined to exclude the possiblity that only the type 2 gene happened to be sequenced.
It emerges from direct tblastn that the Sacrophilus individual sequenced was female. That is, ATRX is well represented but not ATRY (though the situation is somewhat confused due to additional paralogs). Marsupial XY are quite different from placentals:
"Many or most genes on the mammal Y chromosome evolved a testis-specific function after diverging from an X-borne copy with a general function in both sexes. In marsupial but not eutherian mammals, a testis-specific orthologue (ATRY) of the widely expressed X-borne ATRX gene lies on the Y chromosome. Since mutations in human ATRX cause sex reversal, it is possible that one function of ATRY in marsupials is testicular differentiation. We report here the isolation and sequencing of the tammar wallaby (Macropus eugenii) ATRY cDNA, and comparison of its sequence with that of tammar ATRX. The evolution of a testis-specific function for the ATRY protein distinct from the general role of ATRX in both sexes has been accompanied by sequence changes in many protein domains that would alter protein binding partners. A large open reading frame encodes a 1771 amino acid ATRY protein that has diverged extensively from ATRX. The conservation and loss of particular motifs identify those required for testicular function (ATRY) and function in other tissues (ATRX)."
AY532685 MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE Sarcophilus harrisii AY532684 ....E................................S....................P. Dasyurus geoffroii AY532681 ....E................................S....................P. Dasyurus albopunctatus AY532683 ....E................................S....................P. Dasyurus viverrinus AY532682 ....E........................P.......SE...................P. Dasyurus spartacus AY532680 ....E..............R.................SR...................P. Dasyurus maculatus AY532678 ..V..................................S....................P. Dasycercus cristicauda AY532669 ..V..................................S....................P. Dasykaluta rosamondae AY532676 ..V..................S...............S....................P. Myoictis wallacei AY532675 ..V..................S...............S....................P. Myoictis melas AY532687 ..V........N.L.......................S....................P. Neophascogale lorentzii AY532671 ..V..................................S....................P. Parantechinus bilarni AY532670 ..V.................................TS.........RG.........P. Parantechinus apicalis AY532686 ..V..................................S........P...........p. Phascolosorex dorsalis AY532674 ..V.......................................................P. Pseudantechinus ningbing AY532672 ..V..................................S....................P. Pseudantechinus woolleyae AY532673 ..V........N..R......................S...................SP. Pseudantechinus roryi 454 read MEILQKYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAMLQAVSEDP Sarcophilus harrisii EF028739 ............................V.TEEDLAAKLNAMLQA.............P. Antechinus minimus AY243439 ....E..............R........V.TEEDLAAKLNAMLQA.............P. Dasyurus maculatus EF028750 ....K................KT.....I.TEEDLAAKLNAILQA.............P. Myrmecobius fasciatus EF028737 ..V.........................V.TEEDLAAKINAMLQA.............P. Antechinus flavipes EF028748 ..V.........................V.TEEDLAAKLNA.LQA.............P. Sminthopsis crassicaudata AY243438 ..V.........................V.TEEDLAAKLNA.LQA.............P. Planigale sp. EF028749 ..V.........................V.TEEDLAAKLNA.LQA.............P. Planigale ingrami AY532679 ..V.........................V.TEEDLAAKLNAMLQA............... Dasyurus hallucatus AF025382 ..V.........................V.TEEDLAAKLNAMLQA.............P. Phascogale tapoatafa EF028741 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus godmani AY532666 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus swainsonii EF028736 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus stuartii EF028742 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus agilis EF028738 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus bellus EF028740 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus leo EF028747 ..V.........................V.TEEDLAAKLNAMLQA.............P. Phascogale calura EF028744 ..V.........................V.TEEDLAAKLNAMLQA.............P. Murexchinus melanurus EF028743 ..V.........................V.TEEDLAAKLNAMLQA.............P. Micromurexia habbema EU086688 ..V.........................V.TEEDLAAKLNAMLQA.............P. Pseudantechinus macdonnellensis EU086689 ..V.........................V.TEEDLAAKLNAMLQA.............P. Pseudantechinus roryi EU086686 ..V.........................V.TEEDLAAKLNAMLQA............SP. Pseudantechinus macdonnellensis EU086687 ..V.........................V.TEEDLAAKLNAMLQA..........G..P. Pseudantechinus mimulus AY532667 ..V.........................V.TEEDLAAKLNAMLQA.............P. Murexia longicaudata EF028746 ..V.........................V.TEEDLAAKLNAMLQA.............P. Paramurexia rothschildi AY532677 ..V.........................V.TEEDLAAKLNAMLQA.............P. Dasyuroides byrnei EF028745 ..V..........I..............V.TEEDLAAKLNAMLQA.............P. Phascomurexia naso Macropus eugenii assembly sacHar MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE ME+LQ YYTLVDRVPALLHHLTAIDYSS L + ++ VSEDPRLLVRVLR E macEug MEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPE Monodelphis domestica assembly TSSLVLDLQHSSGGEISG sacHar MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE ME+LQ YYTLVDRVPALLHHLTAIDYSS L + ++ VSEDPRLLVRVLR E monDom MEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPE Ornithorhynchus anatinus assembly sacHar EILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE ++L+ YY LVDRVPALL HL A+D SS L + SR SEDPRLLVR L E ornAna DLLRDYYALVDRVPALLRHLAALDLSSVLSEEDLTSRLNAGLQAASEDPRLLVRRLEPE Equus caballus assembly sacHar EILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE E LQ YYTLVDRVPALLHHL ++D+SS + D ++ VSEDPRLLV V+RS+ equCab EALQDYYTLVDRVPALLHHLASMDFSSVVSEDDLVAKLNAGLQAVSEDPRLLVWVVRSK
Rod rhodopsin RHO1 (4+ marsupials)
The optimal wavelength for scotopic (dim light) vision of Sarcophilus is easily predictable provided key tuning residues are covered by the assembly. The 97% match to Sminthopsis and agreement at tuning residues suggests this aspect of vision will be nearly identical between the two species.
>RHO1_homSap Homo sapiens (human) 0 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 1 2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSR 2 1 YIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKE 0 0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQ 0 0 FRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA* 0 >RHO1_monDom Monodelphis domesticus (opossum) Didelphimorphia 0 MNGTEGPNFYVPFSNKTGTVRSPFEEPQYYLADPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTMTLYTSLHGYFVFGPTGCNLEGFFATLG 1 2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIIGVAFTWVMALACAFPPLIGWSR 2 1 YIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPLIVIFFCYGQLVFTVKE 0 0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPIFMTIPAFFAKSSSVYNPVIYIMMNKQ 0 0 FRTCMITTLCCGKNPLGDDEASATASKTETSQVAPA* 0 >RHO1_macEug Macropus eugenii (wallaby) Diprotodontia frag, traces not yet consulted 0 MNGTEGPNFYVPFSNKTGVVRSPFEEPQYYLAEPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLADADLFMDFGGFT 1 2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACSTPPLLGWSR 2 1 0 0 ESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTLPAFFAKTSAVYNPVIYIMMNKQ 0 0 FRNCMITTLCCGKNPLGDDEASATTSKTETSQVAPA* 0 >RHO1_smiCra Sminthopsis crassicaudata (fat-tailed dunnart) Dasyuromorphia 0 MNGTEGPNFYVPYSNKSGVVRSPYEEPQYYLAEPWMFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAVADLFMVICGFTTTLVTSLNGYFVFGTTGCLVEGFFATTG 1 2 GEVALWALVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWIMALACSVPPIFGWSR 2 1 YIPEGMQCSCGIDYYTLNPEFNNESFVIYMFVVHFIIPLTVIFFCYGQLVFTVKE 0 0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQ 0 0 FRNCMITTLCCGKNPLGDDEASTTASKTETSQVAPA* 0 >RHO1_sacHar Sarcophilus harrisii (tasmanian_devil) 97% identity Sminthopsis crassicaudata 0 MNGTEGPNFYVPHSNKTGVVRSPYEEPQYYLAEPWMFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAVADLFMVICGFTTTLVTSLNGYFVFGTTGCQIEGFFATTG 1 2 GEVALWALVVLAIERYIVVCKPMSNFRFGENHAIMGVVFTWIMALACSVPPLFGWSR 2 1 YIPEGMQCSCGIDYYTLNPEFNNESFVIYMFVVHFTIPLTVIFFCYGQLVFTVKE 0 0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQ 0 0 FRTCMITTLCCGKNPLGDDEASATVSKTETSQVAPA* 0 >RHO1_calPhi Caluromys philander (woolly opossum) Didelphimorphia abstract:14659889 0 MNGTEGPNFYVPFSNKTGVVRSPFEEPQYYLAEPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTTTLYTSLHGYFVFGPTGCDLEGFFATLG 1 2 GEIALWSLVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSR 2 1 YIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMVVIFFCYGQLVFTVKE 0 0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPILMTLPAFFAKTSAVYNPVIYIMLNKQ 0 0 FRTCMLTTLCCGKIPLGDDEASATASKTETSQVAPA* >RHO1_ornAna Ornithorhynchus anatinus (platypus) 0 MNGTEGQDFYIPMSNKTGVVRSPFEYPQYYLAEPWQYSVLAAYMFMLIMLGFPINFLTLYVTIQHKKLRTPLNYILLNLAFANHFMVLGGFTTTLYTSLHGYFVFGPTGCNIEGFFATLG 1 2 GEIALWSLVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWIMALACALPPLVGWSR 2 1 YIPEGMQCSCGIDYYTLRPEVNNESFVIYMFVVHFTIPMTIIFFCYGRLVFTVKE 0 0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTVPAFFAKSSAIYNPVIYIMMNKQ 0 0 FRNCMLTTICCGKNPLGDDEASATASKTEQSSVSTSQVSPA* 0
Cone rhodopsin SWS2 (9+ marsupials)
Cone rhodopsin RHO2 has been lost in all mammals and no debris from this gene is expected in Sacrophilus). The short wavelength cone opsin SWS2, while still present in platypus, has also been lost in all theran opsin too long ago to leave detectable remnants in syntenic position. Cone opsin SWS1 has this turned around, being present in theran mammals but only as debris in platypus. A nearly full length gene, most simiilar to Sminthopsis, can be recovered from Sarcophilus read coverage.
>SWS1_homSap Homo sapiens (human) Gt -FAM137A -CALU -NAG6 -FLNC 1385866 NP_990769 cone short 0 MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVA 1 2 GLVTGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSR 2 1 FIPEGLQCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKA 0 0 VAAQQQESATTQKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNPIIYCFMNKQ 0 0 FQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN* 0 >SWS1_monDom Monodelphis domesticus (opossum) Didelphimorphia 0 MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHFQTVFMGFVFCAGTPLNAVVLVATLRYKKLRQPLNYILVNVSLCGFIFCIFAVFTVFISSSQGYFIFGRHVCAMEAFLGSVA 1 2 GLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWVIGIGVSIPPFFGWSR 2 1 FIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIMPLFLICFSYSQLLRALRA 0 0 VAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNQNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0 0 FHACIMEMVCRKPMTDDSDVSSSQKTEVSAVSSSQVGPT* 0 >SWS1_thyEle Thylamys elegans (fat-tailed opossum) Didelphimorphia MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHLQTVFMGFVFC AGTPLNAVVLVATLRYKKLRQPLNYILVNVSFSGFIFCIFAVFTVFISSSQGYFIFGH HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWVIGI GVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLFLICFSY SQLLGALRAVAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNRNH GLDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMVCRKPMTDDSDVSSSQKTE VSAVSSSQVGPS >SWS1_didAur Didelphis aurita (big-eared opossum) Didelphimorphia MSGDEEFYLFKNISSVGPWDGPQHHIAPAWAFHFQTVFMGFVFC AGTPLNAVVLVATLRYKKLRQPLNYILVNVSLSGFIFCIFAVFTVFISSSRGYFVFGR HVCAMEAFLGSVAGLVMGWSLAFLAFERFVVICKPFGNFRFNAKHAMMVVLATWVIGI GVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYAWFLFLSCFIGPLFLICFSY AQLLGALRAVAAQQQESTTTQKAEREVSRMVVMMVGSFCLCYVPYAALGMYMINNRNH GLDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMVCRKPMADDSDITSSQKTE VSTVSSSQVGPS >SWS1_macEug Macropus eugenii (wallaby) Diprotodontia MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHCQTVFMGFVFFAGTPLNAVVLIATFRYKKLRQPLNYILVNISLAGFIYCIFSVFTVFISSSQGYFIFGR HVCAMEGFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFNSKHSMMVVLATWVIGIGVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFILCFIMPLSLICFSY SQLLGALRAVAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNRNHGIDLRLVTIPAFFSKSSCVYNPIIYCFMNKQFHACIMEMVCRKPMTDDSEASSSQKTEVSTVSSSQVGPS* >SWS1_setBra Setonix brachyurus (quokka) Diprotodontia MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHCQTVFMGFVFF AGTPLNAVVLIATFRYKKLRQPLNYILVNISLAGFIYCIISVFTVFISSSQGYFIFGR HVCAMEGFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFNSKHSMMVVLATWVIGI GVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYHSEYYTWFLFILCFIMPLSLICFSY SQLLGALRAVAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNRNH GIDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMVCRKPMTDDSEASSSQKTE VSTVSSSQVGPS >SWS1_tarRos Tarsipes rostratus (honey possum) Diprotodontia MSGDEEFYLFKDISSVGPWDGPQYHIAPAWAFHFQTTFMGFVFF AGTPLNAVVLIATLRYKKLRQPLNYILVNISLAGFIFCVISVFTVFISSSQGYFIFGR HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFSSKHAMMVVLATWVIGI GVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYHSEYYTGFLFIFCFIVPLSLICFSY SQLLGALRAVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNH GLDLRLVTIPAFFSKSACVYNPIVYWFMNKQFHACIMEMVCRKPMTDDSEISSSQKTE VSTVSSSQVGPS >SWS1_cerCon Cercartetus concinnus (pygmy possum) Diprotodontia MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHFQTAFMGFVFF VGTPLNAVVLVATLCYKKLRQPLNYILVNVSLAGFIFCIISVFTVFISSSQGYFIFGR HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFSSKHAMMVVLATWVIGI GVSIPPFFGWSRYIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLICFSY SQLLGALRAVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNH GLDLRLVTIPACFSK >SWS1_smiCra Sminthopsis crassicaudata (dunnart) Dasyuromorphia 0 MSGDEEFYLFKNISLVGPWDGPQYHLAPAWAFHFQTAFMGFVFFAGTSLNGVVLIATLRYKKLRQPLNYILVNISLAGFIFCVFSVFTVFVSSSQGYFVFGRHVCAMEGFLGSVA 1 2 GLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWIIGIGVSIPPFFGWSR 2 1 YIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLICFSYSQLLGALRA 0 0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAAMAMYMVNNRNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0 0 FHACIMEMICKKPMTDDSETTSSQKTEVSTVSSSQVGPS* 0 >SWS1_sacHar Sarcophilus harrisii (tasmanian_devil) part of last exon missing 96% identity Sminthopsis crassicaudata 0 MSGDEEFYLFKNISPVGPWDGPQYHIAPAWAFHLQTAFMGFVFFAGTPLNGVVLIATLRYKKLRQPLNYILVNISLAGFIFCVFSVFTVFVSSSQGYFVFGRHVCAMEGFLGSVA 1 2 SGLVTGWSLAFLAFERFIVICKPFGNFRFHSKHATMVVLATWVIGIGVSIPPFFGWSR 2 1 YIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLICFSYSQLLGALRAVS 0 0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNQ 0 0 KPMTDDSETTSSQKTEVSTVSSSQVGPS* 0 >SWS1_isoObe Isoodon obesulus (bandicoot) Peramelemorphia MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHCQTVFMGFVFF AGTPLNAVVLIATLRYKKLRQPLNYILVNISLAGFIFCIFSVFTVFISSSQGYFIFGR HVCAMEAFLGSVAGLVTGWSLAFLAFERFIVICKPFGNFRFHSKHAMMVVLATWVIGI GVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIIPLSLICFSY SQLLRALRTVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNH GLDLRLVTIPAFFSKSACVYNPIIYCFMNKQFHACIMEMICRKPMTDDSETSSSQKTE VSTVSSSQVSPS >SWS1_galGal Gallus gallus (chicken) Gt 0...2.1.0.0 indel x x x x 348 aa 000 nm no_ref genome cone short1 violet 0 MSSDDDFYLFTNGSVPGPWDGPQYHIAPPWAFYLQTAFMGIVFAVGTPLNAVVLWVTVRYKRLRQPLNYILVNISASGFVSCVLSVFVVFVASARGYFVFGKRVCELEAFVGTHG 1 2 GLVTGWSLAFLAFERYIVICKPFGNFRFSSRHALLVVVATWLIGVGVGLPPFFGWSR 2 1 YMPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRA 0 0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0 0 FRACIMETVCGKPLTDDSDASTSAQRTEVSSVSSSQVGPT* 0
Cone rhodopsin LWS (9+ marsupials)
This basal long wavelength imaging opsin is available from 97 vertebrates and has already been analyzed for phyloSNPs and rare genomic events. The Didelphimorphia experienced a 3-4 residue insert in exon 1 that separates them from all other marsupials. Note this region has quite a complicated indel history. The extra residues have repeat character DVNE DDND suggesting replication slippage. The gene is present and intact in Sarcophilus though two exons are not currently available. LWS in tasmanian devil is identical to the Sminthopsis ortholog.
LWS_loxAfr MAQQWGPHRLTGARLQDASE---DSTQASIFVYTNTNT elephant LWS_echTel MAQRWGAHRLTGGQLQDTYE---GSTRTSIFVYTNSTS tenrec LWS_monDom MTQAWDPAGFLARRRDVNEDDNDETTRSSLFVYTNSNN Didelphimorphia LWS_didAur MTQAWDPVGFLARRRDENEDDHDDTTRASLFVYTNSNN Didelphimorphia LWS_tarRos MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Diprotodontia LWS_macEug MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Diprotodontia LWS_smiCra MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Dasyuromorphia LWS_sacHar MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Dasyuromorphia LWS_setBra MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Diprotodontia LWS_cerCon MTQAWDPAGFLAWQEDENE----ETTRASLFVYTNSNN Diprotodontia LWS_myrFas MTQAWDPAGFLAWRREENE----ETTRASLFTYTNSNN Dasyuromorphia LWS_isoObe MTQAWDPAGFLAWRRDENE----ETTRASLFVYTNSNN Peramelemorphia LWS_ornAna MTPAWNSGVYAARRRFEDEE---DTTRTSVFVYTNSNN platupus LWS_tacAcu MTQAWDPAGFLAWRRDENEE---TTRASLFVYTNSNNT echidna
>LWS_homSap Homo sapiens (human) 0 MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTR 1 2 GPFEGPNYHIAPRWVYHLTSVWMIFVVIASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISVVNQVYGYFVLGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWMVVCKPFGNVRFDAKLAIVGIAFSWIWAAVWTAPPIFGWSR 2 1 YWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCITPLSIIVLCYLQVWLAIRA 0 0 VAKQQKESESTQKAEKEVTRMVVVMVLAFCFCWGPYAFFACFAAANPGYPFHPLMAALPAFFAKSATIYNPVIYVFMNRQ 0 0 FRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA* 0 >LWS_monDom Monodelphis domesticus (opossum) Didelphimorphia 4aa insert 0 MTQAWDPAGFLARRRDVNEDDNDETTRSSLFVYTNSNNTR 1 2 GPFEGPNYHIAPRWVYNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETVIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAAVWTAPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMATCCIFPLSIILLCYVQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYSFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSKTEGSSVSSVAPA* 0 >LWS_didAur Didelphis aurita (big-eared opossum) Didelphimorphia 4aa insert 0 MTQAWDPVGFLARRRDENEDDHDDTTRASLFVYTNSNNTR 1 2 GPFEGPNYHIAPRWVYNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETVIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAAVWTSPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDLGVQSYMIVLMATCCIFPLSIILLCYIQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSKTEVSSVSSVAPA* 0 >LWS_tarRos Tarsipes rostratus (honey possum) Diprotodontia ENED insert 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTR 1 2 GPFEGPNYHIAPRWVFNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAIWTSPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGNSDPGIQSYMIVLMSTCCILPLSIILLCYVQVWRAIRA 2 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0 >LWS_macEug Macropus eugenii (wallaby) Diprotodontia 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1 2 GPFEGPNYHIAPRWVFNLTSLWMIFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGNSDPGVQSYMIVLMSTCCILPLSVIFLCYIQVWLAIRS 2 0 VAKQQKESESTQKAEKEVSRMVVVMILAFCFCWGPYAIFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0 >LWS_setBra Setonix brachyurus (quokka) Diprotodonti 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1 2 GPFEGPNYHIAPRWVFNLTSLWMIFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETMIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGNSDPGVQSYMIVLMSTCCILPLSVILLCYIQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYAIFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVPA* 0 >LWS_cerCon Cercartetus concinnus (pygmy possum) Diprotodontia 0 MTQAWDPAGFLAWQEDENEETTRASLFVYTNSNNTK 1 2 GPFEGPNYHIAPRWVFNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAIADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAIWTSPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGNSDPGIQSYMIVLMSTCCILPLSIILLCYIQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTFFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* >LWS_smiCra Sminthopsis crassicaudata (dunnart) Dasyuromorphia 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1 2 GPFEGPNYHIAPRWVYNLTSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIILCYIQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVMVMILAFCFCWGPYALFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0 >LWS_sacHar Sarcophilus harrisii (tasmanian_devil) half of exon 2, all of exon 4 missing frag 100% identical to Sminthopsis 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTK 1 2 FKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIILCYIQVWLAIRA 0 0 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0 >LWS_myrFas Myrmecobius fasciatus (numbat) Dasyuromorphia 0 MTQAWDPAGFLAWRREENEETTRASLFTYTNSNNTK 1 2 GPFEGPNYHIAPRWVYNLTSFWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSVILLCYIQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYAIFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0 >LWS_isoObe Isoodon obesulus (bandicoot) Peramelemorphia 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTR 1 2 GPFEGPNYHIAPRWVYNLTSFWMFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMTTCCILPLSIILLCYVQVWLAIRA 0 0 VAKQQKDSESTQKAEKEVSRMVVVMIRAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSGTSRTEVSSVSSAPA* 0 >LWS_ornAna Ornithorhynchus anatinus (platypus) 0 MTPAWNSGVYAARRRFEDEEDTTRTSVFVYTNSNNTR 1 2 DPFEGPNYHIAPRWAYNVTSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIFGYFILGHPMCVLEGYTVSLC 1 2 GITGLWSLSIISWERWIVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIVLCYLQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTIFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0 0 FRNCIMQLFGKKVDDGSELSSTSRTEVSSVSSVSPA* 0 >LWS_tacAcu Tachyglossus aculeatus (echidna) 0 MTQAWDPAGFLAWRRDENEETTRASLFVYTNSNNTR 1 2 GPFEGPNYHIAPRWVFNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETIIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1 2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAVWTSPPLFGWSR 2 1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMATCCIFPLSIILLCYIQVWLAIRA 0 0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYAFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0 0 FRTCILQLFGKKVDDGSEVSSTSKTEVSSVSSVAPA* 0
Encephalopsin (2+ marsupials)
Pinopsin, parapinopsin, parietopsinand VA opsin all terminate in sauropods and are missing in all mammals. Encephalopsin has a very peculiar history of gene loss in tetrapods, requiring some seven independent and asynchronous events including platypus. While this limits the phylogenetic utility of any gene loss within marsupials, the status of the gene within Sarcophilus is still informative. A full length gene can be recovered with 94% identity to opossum, strongly indicating that encephalopsin is fully functional within Sarcophilus.
>ENCEPH_homSap Homo sapiens (human) OPN3 0 MYSGNRSGGHGYWDGGGAAGAEGPAPAGTLSPAPLFSPGTYERLALLLGSIGLLGVGNNLLVLVLYYKFQRLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLF 1 2 GIVSIATLTVLAYERYIRVVHARVINFSWAWRAITYIWLYSLAWAGAPLLGWNRYILDVHGLGCTVDWKSKDANDSSFVLFLFLGCLVVPLGVIAHCYGHILYSIRM 0 0 LRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVNGHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRK 0 0 FRRSLLQLLCLRLLRCQRPAKDLPAAGSEMQIRPIVMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVDDSDKTNGSKVDVIQVRPL* 0 >ENCEPH_monDom Monodelphis domestica (opossum) 0 MYSDNSSDDGGGGYWGSGRAGGASGTGVTGEPGPEGSPRQAPLFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFNDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF 1 2 GIVSIMTLTVLAYERYNRIVHAKVINFSWAWRAITYIWLYSLVWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVIFLFFGCLMLPVGVMAYCYGHILYAIRM 0 LRCVEELQTIQVIKILRYEKKVAKMCFLMIAIFLFCWMPYAVICLLVANGYGSLVTPTVAIIASLFAKSSTAYNPIIYIFMSRK 0 0 FRRCLLQLLCFRLLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDENDKNSGTKVNVIQVRPL* 0 >ENCEPH_sacHar Sarcophilus harrisii (tasmanian_devil) 94% identity monDom 0 MYSGNSSDDAGGGYWGSGGTGGAGGTGVAGEPAPEGSPRPAPLFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFSDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF 1 2 GIVSIMTLTVLAYERYNRIVHAKVINFSWAWRAITYIWLYSLIWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVLFLFLGCLVLPVGVMAYCYGHILYAIRM 0 0 FRCVEELQTLQVIKILRYEKKVAKMCFLMIATFLFCWMPHAVICFLVANGYGSLVTPTVAIIPSLFAKSSTAYNPIIYIFMSRK 0 0 FRRCLLQLLcFRQLKFQQPKKDRAIIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDDNDKNSETKVNVIQVRPL* 0 >ENCEPH_macEug Macropus eugenii frag 0 GALGCREPGQREPSSSAPFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLLLVNISFSDLLVSLFGVTFTFVSCLRSGWVWHTVGCAWDGFSNSLF 1 2 GIVSIMTLTVLAYERYHRIVHAKVINFSWTWRAITYIWLYSLVWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVLFLFLGCLVLPVGVMAYCYGHILYAIRM 0 0 0 0 FRRCLLQLLCFRQLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDDNDKNNGTKVNVIQVRPL* 0 >ENCEPH_ornAna Ornithorhynchus anatinus pseudo 0 MVPWNGS-GRHLGAVR---GPE--SLPATPGAARPSRPGAGDGRL--LGLF-P-GVGGNLLVLLL--ALPGPPTTTDLYLASVAVSDLL--LL---LPFVYRLWRSRPWVFVCRLLGE-GGSLA 1 2 GIVSLISLAVLSYERYTLTLHPKQSNYQKAVLAVGASWIYSLIWTIPPLLGWSSYGTEGAGTSCSVHWSSKSVC-SYIVCLFI--CLVIPVLVMIYCYGRLLYAVKQ 0 0 LHCVKELQNIQVIGSLRYER*VTEMYFFTIAQFLVCQSPSALVSYPAAH-----VSPVVAKISPVFANSSFVYNPVISIFVRRK 0 0 KASR*KVNVIQVQPPS* 0 >ENCEPH_galGal Gallus gallus (chicken) 71%=homSap encephalopsin OPN3 full 0 MHSGNGTGATSRPQLAAAGHEVPGERPLFSAGTYELLALLIATIGTLGVCNNLLVLVLYYKFKRLRTPTNLFLVNISLSDLLVSVCGVSLTFMSCLRSRWVWDAAGCVWDGFSNSLF 1 2 GIVSIMTLTVLAYERYIRVVHAKVIDFSWSWRAITYIWLYSLAWTGAPLLGWNRYTLEIHGLGCSMDWKSKDPNDTSFVLLFFLGCLVAPVVIMAYCYGHILYAVRM 0 0 LRCVEDFQTSQVIKLLKYEKKVAKMCFLMISTFLICWMPYAVVSLLVTYGYSNLVTPTVAIIPSFFAKSSTAYNPVIYIFMSRK 0 0 FRQCLLQLLCFRLMRFQRIMKEPSGAGNVKPIRPIVMSQKVGDRPKKKVTFSSSSIIFIIASDDTQQIDDNSKHNGTKVNVIQVKPL* 0
TMT opsin (2+ marsupials)
TMT is an ancient locus that is present in monotremes and marsupials but lost in all placentals.
>TMT_monDom Monodelphis domestica shortened final exon 0 MSNNLTTNLSLEALLSASEDKQRNGLSRTGHTIVAVFLGIILIFGSISNFIVLVLFCKFKVLRNPVNMLLLNISISDMLVCLSGTTLSFASSIQGRWIGGKHGCRWYGFANSCF 1 2 GIVSLISLAILSYERYRTLTLCPGQGADYQKALLAVAGSWLYSLVWTVPPLIGWSSYGTEGAGTSCSVHWTSKSVESVSYIMCLFIFCLVIPILVMVYFYGRLLYAVKQ 0 0 VGKIRKTAARKREYHVLFMVVTAVICYLICWVPYGMIALLATFGPPGVVSPVANVVPSILAKSSTVCNPIIYVLMNKQ 0 0 FYKCFLILFHCQPAQSGPDVSLCPSNVTVIQLGQRKNKDAPGSI* >TMT_macEug Macropus eugenii frag 0 MSINLTANLSFGTLLPDSEEKQRSGLSRTGHTVTAVFLGLILILGVINNFIVLVLFCKFKVLRNPVNMLLLNISISDMLVCLTGTTLSFASSIRGRWIAGYHGCRWYGFANSCF 1 2 GIVSLISLAVLSYERYRTLTLCPRQGTDYHKALLAVAGSWLYSLIWTVPPLIGWSSYGTEGAGTSCSVHWTSKSVESVSYIMCLFIFCLVIPILFMVYFYGRLLYTVKQ 0 0 VGKIRKSAARKREYHVLFMVVTAVICYLICWVPYGMIALLATFGPPGVVSPVANVVPSILAKSSTVCNPIIYILMNKQ 0 0 FYKCFLILFHCQPASSASDASLCPSKMTVIQLGQRKDKEVPCAIQDLPEVSKKQLCLLSPESNVAPSSGHPQEKMEEKPLSE* 0 >TMT_sacHar Sarcophilus harrisii (tasmanian_devil) FP5MBH101BETOZ needed to finish 0 MSINLTTNLSFGPLLIDSEEKPRSGLSRTGHTVVAVFLGIILILGFINNFIVLILFCKFKVLRNPVNMLLLNISISDMLVCLSGTTLSFASSIRGRWIGGYHGCRWYGFANSCF 1 2 GIVSLISLAILSYERYRTLTLCPRRGADYQKALLAVAGSWLYSLIWTVPPLIGWSSYGTEGAGTSCSVHWTSKSVQSVSYIMCLFIFCLVIPILIMIYFYGRLLYTVKQ 0 0 VGKIRKTAARKREYHVLFMVVTAVICYLICWVPYGLIALVATFGPPGVVSPVANIVPSILAKSSTVCNPIIYILMNKQ 0 0 FYKCFLILFHCQPASSAPDASLCPSKVTVIQLGQR * 0 >TMT_ornAna Ornithorhynchus anatinus frag 0 GLSRTGHTMVAVFLGIILVFGFMNNLIVLILFCKFKALRNPVNMIMLNISASDMLVCVSGTTLSFASNISGRWIGGDPGCRWYGFVNSCL 1 2 GIVSLISLAVLSYERYRTLTLHPKQSTDYQKAVLAVGASWIYSLIWTIPPLLGWSSYGTEGAGTSCSVHWSSKSPVSVSYIVCLFIFCLVIPVLVMIYCYGRLLYAVKQ 0 0 IGKARKTAARKREYHVLFMVITTVICYLVCWMPYGVTALLATFGQPGTVSPEASVIPSILAKSSTVCNPIIYILMNKQ 0 0 FYKCFLILFHCQPPRAADAPSTYPSQVMVIQLNQRRSRETAGAPQVLLEMKHQTLHLLGPQLHETPSWERSTPVHPE* 0 >TMT_galGal Gallus gallus 0 MNHTWTYNLSFGAPTDPVEPRAGLSRNGHTVVAVFLGFILFFGFLNNLIVLILFCKFKTLRNPVNMLLLNIhttp://genomewiki.ucsc.edu/index.php?title=Opsin_evolution:_Encephalopsin_gene_loss&action=editSISDMLVCISGTTLSFASNIHGKWIGGEHGCRWYGFVNSCF 1 2 GIVSLISLAVLSYERYSTLTLCNKRSDDYRKALLAVGGSWVYSLLWTVPPLLGWSSYGIEGAGTSCSVRWSSETAESTSYIICLFIFCLVIPVMVMMYCYGRLLYAVKQ 0 0 VGKIHKNTARKREYHVLFMVITTVICYLVCWIPYGVIALLATFGKPGVVTPVASIIPSILAKSSTVCNPIIYILMNKQ 0 0 FYKCFRQLFHCQPPSSTDGEPTCHSKVTVIQLNQKTDGGKLCNNKPRPETDNKVTSLLHPEPGLEPAAKTVPPM* 0 >TMT_taeGut Taeniopygia guttata 0 MNHTWMYNLSFGAPAHPVEPRAGLSRSGHTVVAVFLGLILFFGFLNNLIVLILFCKFKTLRNPVNMLLLNISVSDMLVCISGTTLSFASNIRGKWIGGDHACRWYGFVNSCF 1 2 GVVSLISLAVLSYERYNTLTLCHKRSDDFRKALLAVAGSWIYSLVWTVPPLLGWSSYGVEGAGTSCSVRWSSESAESTSYIICLFVFCLVVPVMVMMYCYGRLLYAVKQ 0 0 VGKIHKNAARKREYHVLFMVIPTVICYLVCWIPYGVIALLATFGKPGAVTPITSIIPSILAKSSTVCNPIIYILMNKQ 0 0 FYKCFRQLFHCQPPSSTDGEPTCHSKVTVIQLDQRADGGNMCNNEPHPETDSKMTSLLCPETTSKATPPTS* 0 >TMT_anoCar full +TMT -ST6GAL2 (overlap) +SLC5A7 0 MSELSSNLTFNMSTSIEEPGSGLSRMGHNIVAVFLGLILVFGFLNNLVVLILFCKFKTLRNPVNMLLLNISASDMLVCISGTTLSFVSNIYGRWIGGEHGCRWYGFVNSCF 1 2 GIVSLISLAILSYERYSTLTQTNKRGSDYQKALLGVGGSWLYSLIWTVPPLIGWSSYGLEGAGTSCSVRWTSETLESVTYIICLFIFCLAIPVLVMIYCYARLFYAVKQ 0 0 VGKLRKTSARKREFHVLFMIITTIICYLICWMPYGVIALLATFGRPGLVSPVASVIPSILAKSSTVFNPIIYILMNKQ 0 0 FYKCFLMLLHCQPSSVADGETICQSKVMAIHQNQKAQGGVILKSQVVPQMDEKAICLLSPESSLDPVLESTPQLSKENSFL* 0 >TMT_xenTro full -UXS1 +TMT -ST6GAL2 (overlap) +SLC5A7 0 MSTIKNWTTNISVENSMSYIENDLSLPTEAVLSRTGHTVVAIFLGFILIFGFLNNFVVLILFCKFKTLRTPVNMMLLNISASDMLVCVSGTTLSFTSSIKGKWIGGEYGCQWYGFVNSCF 1 2 GIVSLISLAILSYERYSTLTLYNKGGPNFKKALLAVASSWLYSLVWTVPPLLGWSSYGREGAGTSCSVRWTSESVESVSYIICLFIFCLALPVFVMLYCYGRLLYAVKQ 0 0 VGKIRKIAARKREYHVLFMVITTVICYLLCWLPYGVVALLATFGRPGVISPVASVVPSILAKSSTVFNPIIYILMNKQ 0 0 FYKCFLILFHCHPTSSADGKSICQSNYTVIQLNQKLNNIVAIPGQTQIPESVDKMPCIHRQNNESPSDQMPQSTTEHLISGT* 0
RGR opsin (0 marsupials)
This gene has apparently been lost specifically in the marsupial clade, though support for that is only provided by the Monodelphis and Macropus genome projects. It would be of considerable interest to find the gene or a fragment thereof in syntenic position in Sarcophilus. However nothing can be found with tblastn of current reads.
>RGR1_homSap Homo sapiens (human) +PCDH21 -LRIT1 -GRID1 -WAPAL NM_001012720 retinal epithelium Mueller 0 MAETSALPTGFGELEVLAVGMVLLVE 1 2 ALSGLSLNTLTIFSFCKTPELRTPCHLLVLSLALADSGISLNALVAATSSLLR 2 1 RWPYGSDGCQAHGFQGFVTALASICSSAAIAWGRYHHYCT 1 2 RSQLAWNSAVSLVLFVWLSSAFWAALPLLGWGHYDYEPLGTCCTLDYSKGDR 2 1 NFTSFLFTMSFFNFAMPLFITITSYSLMEQKLGKSGHLQ 0 0 VNTTLPARTLLLGWGPYAILYLYAVIADVTSISPKLQM 0 0 VPALIAKMVPTINAINYALGNEMVCRGIWQCLSPQKREKDRTK* 0 >RGR_dasNov Dasypus novemcinctus (armadillo) 0 MAGSGVLPPGFGELEVLAVGTVLLVE 1 2 ALSGLVLNGLAIISFCKTPELRSPSRLLVLSLALADSGVSLNALVAATSSLLR 2 1 RWPYGSGGCQAHGFQGFVTALASISSSAAIAWERCHRHCI 1 2 GRRLAWSTAGCLVLCLWMAAAFWAALPLLGWGLYDYEPLGTCCTLDYSRGDR 2 1 NFISFLVTLALFNFFLPLLIMLTSYRLMAQKLKRSGHVQ 0 0 VSTALPGRLLLLGWGPYALLYLYAAVADATSLSPRLQM 0 0 VPALIAKTMPTVNALYYALGRESVHRNA* 0 >RGR_loxAfr Loxodonta africana (elephant) 0 MAEPGHLPAGFQELEVLTVGTVLLLE 1 2 ALSGLSLNGLTILSFCKIPELRTPGHLLVLSLALADSGISLNALVAAMSSLRR 2 1 RWPYGSDGCQAHGFQGFVTALASICSCAAIAWERYHHYCT 1 2 RSRLAWSSASALVLFVWLSSAFWAALPLLGWGRYNYEPLGTCCTLDYSRGDR 2 1 NSTSFLLTMAFFNFLLPLFITLTSYRLMEQKLKKKGPLQ 0 0 VNTTLPARTLLLGWGPYALLYLCAAATDMTSISPRLQM 0 0 VPALVAKAVPVINACHYALGSEVVRGGIWQYLSRQRGESPLRARDRTH* 0 >RGR1_ornAna Ornithorhynchus anatinus (platypus) missing exon 1 DRY motif, afros ERY, other placentals GRY 0 1 2 ALLGLCLNGLTIASFRKIKELRTPSNLLVVSLALADSGICLNALMAALSSFLR 2 1 HWPYGAEGCRLHGFQGFATALASISLSAAIGWDRYLRHCS 1 2 RSKPQWGTAVSTVLFAWGFSAFWSMMPILGWGQYDYEPLRTCCTLDYSKGDR 2 1 NFTTYLFAVAFFNFVIPLFIMLTSYQSIEQRFKKSGLFK 0 0 LNTRLPTRTLLFCWGPYALLCFYATVENVTFISPKLRM 0 0 IPALIAKTVPVIDAFTYALRNEDYRGGIWQFLTGQKIERVEVENKIK* 0 >RGR1_galGal Gallus gallus (chicken) +PCDH21 -LRIT1 +CHAT -PARG 14985289 NM_001031216 0 MVTSHPLPEGFTEIEVFAIGTALLVE 1 2 ALLGFCLNGLTIISFRKIKELRTPSNLLVLSIALADCGICINAFIAAFSSFLR 2 1 YWPYGSEGCQIHGFQGFLTALASISSSAAVAWDRYHHYCT 1 2 RSKLQWSTAISMMVFAWLFAAFWATMPLLGWGEYDYEPLRTCCTLDYSKGDR 2 1 NYITFLFALSIFNFMIPGFIMMTAYQSIHQKFKKSGHYK 0 0 FNTGLPLKTLVICWGPYCLLSFYAAIENVMFISPKYRM 0 0 IPAIIAKTVPTVDSFVYALGNENYRGGIWQFLTGQKIEKAEVDSKTK* 0 >RGR1_xenTro Xenopus tropicalis (frog) ?? 0.2.1.2.1.0.0 indel +PCDH21 -LRIT1 +CHAT -PARG 296 BC135113 0 MVTSYPLPEGFTETEVFAIGTTLLVE 0 0 ALLGLLLNGLTLLSFYKIRELRTPSNLFIISLAVADTGLCLNAFVAAFSSFLR 2 1 YWPYGSEGCQIHGFQGFVAALSSIGSCAAIAWDRYHQYCT 1 2 RSKLHWSTAVSVVFFIWGFSAFWSAMPLFGWGEYDYEPLRTCCTLDYSKGDR 2 1 NYISYLFTMAFFEFLVPLFILMTAYQSIYQKMKKSGQIR 0 0 FNTSMPVKSLVFCWGPYCLLCFYAVIQDATILSPKLRM 0 0 IPALLAKTSPAVNAYVYGLGNENYRGGIWQYLTGQKLEKAETDNKTK* 0
Peropsin (2+ marsupials)
Sarophillus can be expected to have this gene. Further, the protein sequence should substantiate the 4 previously defined phyloSNPs characteristic of the marsupial/placental transition.
>PER_homSap Homo sapiens (human) 0 MLRNNLGNSSDSKNEDGSVFSQTEHNIVATYLIMA 1 2 GMISIISNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQ 0 0 VYAGLNIFFGMASIGLLTVVAVDRYLTICLPDV 1 2 GRRMTTNTYIGLILGAWINGLFWALMPIIGWASYAPDPTGATCTINWRKNDR 2 1 SFVSYTMTVIAINFIVPLTVMFYCYYHVTLSIKHHTTSDCTESLNRDWSDQIDVTK 0 0 MSVIMICMFLVAWSPYSIVCLWASFGDPKKIPPPMAIIAPLFAKSSTFYNPCIYVVANKK 2 1 FRRAMLAMFKCQTHQTMPVTSILPMDVSQNPLASGRI* 0 >PER_loxAfr Loxodonta africana (elephant) 0 MLRNSLDNSSDSKNEDASVFSQTEHNIVATYLIMA 1 2 GMISILSNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLHGRWKFGYTGCQ 0 0 IYAGLNIFFGMASIGLLTVVAVDRYLTICHPHI 1 2 GRRMTSNTYVSMILGAWINGLLWALLPITGWASYAPDPTGATCTINWRKNDA 2 1 SFVSYTMTVIVINFVVPLAVMFYCYYHVTRSIKRHTASNCAEYLNRDWSDQLDVTK 0 0 MSVIMILMFLVAWSPYSIVCLWASFGDSKKIPPSMAIIAPLFAKSSTFYNPCIYVVANKK 2 1 FRRAMFAMFKCQTHQAEPVTCILPMNVSQNPLAAGRI* 0 >PER_monDom Monodelphis domestica (opossum) 0 MFKNNSVKTLAPEKEGPSVFSPIEHKIVAAYLITA 1 2 GVISIVSNVIVLGIFVKYKALRTATNTIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYDGCQ 0 0 IYAGLNIFFGMASIGLLTAVAIDRYLTICQPDL 1 2 GGRMTSYNYTLMILTAWVNGFFWALMPIVGWAGYAPDPTGATCTINWRKNDV 2 1 SFVSYTMTVITINFAMPLGVMFYCYYNVSQKMKQYSPSNCPDHINRDWSNQVAVTK 0 0 MSVVMILMFLLAWSPYSIVCLWASFGDPKEIPPAMAIVAPLFAKSSTFYNPCIYVAANKK 2 1 FRRAISAMIRCQTHQSMPISNALPMN* 0 >PER_macEug Macropus eugenii (wallaby) 0 MFQNDSLEPEKESYSVFSPTEHNIVAAYLITA 1 2 GVISIPSNIIVLGIFVKYKELRTATNTIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQ 0 0 IYAGLNIFFGMASIGLLTAVAIDRYLTICQPDL 1 2 2 1 SFVSYTMTVIAINFVMPLVVMFYCYYNVSLKMKQYTRSSCPEHINRDWSNQVDVTK 0 0 MSVIMILMFLLAWSPYSVVCLWASFGDPKEIPPAMAIIAPLFAKSSTFYNPCIYVAANKK 2 1 FRRAISAMMRCETHQSMPVSNALPLNLT* 0 >PER_sarHar Sarcophilus harrisii (tasmanian_devil) 5.5 of 7 exons 0 MFKNDSFRSLEPEKEGHSVFSPAEHNIVAAYLITA 1 2 SILSNVIVLGIFVKFKELRTATNAIIINLA 0 0 1 2 GRRMTSFNYTIMILTAWVNGFFWALMPIVGWASYAPDPTGA 2 1 SFVSYTVTVIAINFVMPLVVMIYCYYNVSQKIKQYTPSNCPEYINRDWSNEVAVTK 0 0 MSVIMILMFLLAWSPYSVVCLWASFGDPKEIPPAMAIIAPLFAKSSTFYNPCIYVAANKK 2 1 FRrAISAMIQCQTHQSMSVSKALPMN* 0 >PER_ornAna Ornithorhynchus anatinus (platypus) 0 MRRNDSANLLESEHHDRSAFSQTDHNIVAAYLITA 1 2 GIMSIVSNVIVLGIFVKFEELRTATNAIIINLAVTDIGVSGIGYPMSAASDLHGSWKFGHAGCQ 0 0 IYAGLNIFFGMSSIGLLTVVAVDRYLTICRPAI 1 2 GRKMTRSNYTAMILAAWMNGFFWASMPLLGWASYASDPTGATCTINWRKNDA 2 1 SFISYTMTVIAVNFAVPLIVMFYCYYNVSKAMRQYPASRVLENLNIDWSEQVDVTK 0 0 MSVVMILMFLMAWSPYSIVCLWSSFGDPKKISPAVAIMAPLFAKSSTFYNPCIYVVANKK 2 1 FRRAMLSMVQCQTHREITITDVLPMNRSRSPLTL* 0 >PER_galGal Gallus gallus (chicken) 0 MHWNDSANSSESDAEAHSVFTQTEHNIVAAYLITA 1 2 GVISIFSNIVVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0 0 IYAALNIFFGMASIGLLTVVAVDRYLTICRPDI 1 2 GRRMTTRNYAALILAAWINAVFWASMPTVGWAGYASDPTGATCTANWRKNDV 2 1 SFVSYTMSVIAVNFVVPLTVMFYCYYNVSRTMKQYTSSNCLESINMDWSDQVDVTK 0 0 MSVVMIVMFLVAWSPYSIVCLWSSFGDPKKISPAMAIIAPLFAKSSTFYNPCIYVIANKK 2 1 FRRAILAMVRCQTRQEITISNALPMTVSLSALTS* 0 >PER_taeGut Taeniopygia guttata (finch) 0 MHWNDSSNSSESDDEAHSAFTQTEHNIVAAYLITA 1 2 GVISIFSNIVVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0 0 IYAALNIFFGMASIGLLTVVAVDRYLTICRPDI 1 2 GRRMTTRSYATLILAAWINAVFWSSMPTAGWASYAPDPTGATCTVNWRKNDA 2 1 SFISYTMSVIAVNFVVPLTVMFYCYYNVSRTMKQYASSNCLESINIDWSDQVDVTK 0 0 MSVVMIIMFLVAWSPYSIVCLWSSFGDPKKISPAMAIIAPLFAKSSTFYNPCIYVIANKK 2 1 FRRAILAMVRCQTRQEITINNALPMSVSQSALTSQNSSHLPA* 0 >PER_anoCar Anolis carolinensis (lizard) 0 MFLNDSANSSESDDEPHSAFSQAEHNIVAAYLITA 1 2 GVISLLSNIVVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0 0 IYAALNIFFGMASIGLLTVVAIDRYLTICKPHI 1 2 GSRLTATNYTTLILAAWINALFWASMPVVGWASYAPDPTGATCTVNWRKNDT 2 1 SFVSYTMSVIAVNFVIPLSVMFYCYYNVSKTMKYYMRNSCLENINIDWSDQVDVTK 0 0 MSVVMIIMFLLAWSPYSIVCLWSSFGDPKKISPAMAIVAPLFAKSSTFYNPCIYVIANKR 2 1 FRRAILAMIRCQTRQEITINNVLPMSVSQSTIA* 0 >PER_xenTro Xenopus tropicalis (frog) 0 METLAEVSTLLPAGTGTVNISDASSEVHSVFSQSEHNIVAAYLITA 1 2 GVISILSNIIVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYVGCQ 0 0 IYAGLNIFFGMASIGLLTVVAIDRYLTICRPDI 1 2 GRRISGRHYTAMILAAWINAVFWSVMPVVGWSSYAPDPTGATCTINWRKNDV 2 1 SFVSYTMSVVAVNFVVPLMVMFYCYYNVSRTMKGYGSRSSLGGINADWSDQTDVTK 0 0 MSMVMIVMFLVAWSPYSIVCLWSSFGDPRKIPPAMAIIAPLFAKSSTFYNPCIYVIANKK 2 1 FRRAILSMVQCKSRQEVTLDNHFPMNVSQSTLTT* 0
Neuropsin (2+ marsupials)
Here Sarcophilus can be predicted to contain only NEUR1 because the ancient vertebrate genes NEUR2 and NEUR3 appear to terminate in sauropods and NEUR4 in platypus.
>NEUR1_homSap Homo sapiens (human) OPN5 0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1 2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1 2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1 2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0 0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFR 2 1 LHTVTTVRKSSAVLEIHEEV* 0 >NEUR1_dasNov 0 MALNHTALPQDDRLPHYLRDGDPFASKLSWEADLVAGFYLTII 1 2 gILSTFGNGYVLYMSSKRKKKLRPAEIMTINLAVCDLGIS 1 2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1 2 GVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0 0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLRATKKKSLEDFR 2 1 LHTVTTVRESSAVLEVHQEV* 0 >NEUR1_monDom 0 MALNHSVSPQDDYIPHYLRDGDPFASKLSWEADLVAGFYLTII 1 2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1 2 VVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1 2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0 0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKAAKKESLRTYR 2 1 LHTVTTVRRSSAVLEIHQEv* 0 >NEUR1_macEug 0 1 2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1 2 VVGKPFTIISCFCHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSy 1 2 GTWLKRHHAYICLVIIWAYATFWATMPLAGLGNYAPEPFGTSCTLDWWLAQASVTGQTFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQSSHVLEMKLTK 0 0 2 1 RHTVSTIRKSSSVSETYQEV* 0 >NEUR1_sarHar Sarcophilus harrisii (tasmanian_devil) 4 of 6 exons 0 1 2 GVLSTLGNGYVIYMSSKRKKKLRPAEIMTVNLAVCDLGIS 1 2 VVGKPFTIISCFSHRWVFGWVGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1 2 GTWLKRHHAYICLVIIWAYATFWATMPLAGLGNYAPEPFGTSCTLDWWLAQASVTGQTFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHSHVLEMKLTK 0 0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPVQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCQSGGQKAAKKESLRDYR 2 1 * 0 >NEUR1_ornAna 0 MTNYSAPQLGDYLPHYLREGDPFVSKLSWEADLVAGVYLVII 1 2 GVLSTLGNGYVIYMSSRRKKKLRPAEIMTVNLAVCDLGIS 1 2 VVGKPFTIVSCFCHRWVFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLSY 1 2 GTWLKRHHAYICLAIIWAYASFWATMPLVGLGNYAPEPFGTSCTLDWWLAQASVAGQAFILNILFFCLLLPTAVIVFSYVKIIAKVKSSTKEVAHFDSRIQNSHVLEMKLTK 0 0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSIPIQFSVVPTLLAKSAAMYNPIIYQVIDCRISCCRLGGPKTGKKESLKNSR 2 1 SHSMSTIRKPSAVSGPHQEV* 0 >NEUR1_galGal 0 MASDCNSSSQEEYLPHYMQQEDPFASKLSREADIIAGFYLTVI 1 2 GILSTLGNGYVIFMSSKRKKKLRPAEIMTVNLAVCDLGIS 1 2 VVGKPFSIISFFSHRWIFGWMGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICHLAY 1 2 GTWLKRHHAFICLALIWAYATFWATVPFAGVGSYAPEPFGTSCTLDWWLAQASVAGQAFVLSILFFCLLFPTAVIVFSYVKIILKVKSSTKEVAHYDTRIQNSHILEMKLTK 0 0 VAMLICAGFLIAWIPYAVVSVWSAFGQPDSVPIQFSVVPTLLAKSAAMYNPIIYQVIDCKFACCRSGGPKTLQKKSSLKESR 2 1 MYTISSHRDSAALSGTQLEV* 0 >NEUR2_galGal Gallus gallus GenBank 5'UTR mistranslated as coding -B4GALT6 -NEUR2_galGal -KIAA1012 0 MDPSFANSTFQSKITEAADIVVGTCYMVF 1 2 GICSLCGNSILLYISYKKKHLLKPAEYFIINLAISDLAMTLTLYPLAVTSSLSHR 2 1 WLYGKHICLFYAFCGLFFGICSLSTLTLLSVVCCLKICFPAY 1 2 GNRFRRKHGQILIACAWTYAAIFACSPLAHWGEYGEEPYGTACCIDWQSTNVDVMSMSYTVVLFVLCFILPCGVIVTSYSLILVTVKESRKAVEQHVSGPTRINNVQTITAK 0 0 LSIAVCIGFFAAWSPYAIIAMWAAFGSIDKIPPLAFAIPAVFAKSSTLYNPIIHLLLKPNFRSNIAKDFTVIQQLCVRCCFCVKELQTYRSTFNTGLRTFKGKNESSCNALPIMEG CSYFPSEKGSHTFECFKSYPNCFQERLSTMGCHLQDCESLENDLQVEVTQGSRNSMKVVEQEEKSTELDNLEITLEAVPVSCTFTDL* 0 >NEUR3_galGal Gallus gallus cOpn5L2 mRMA for Opsin 5-like 2 AB368183 chr3 XM_420056 CN231992 testis exon 2^3 rel NEUR1/2 0 MEEQYISKLHPVVDYGAGVFLLII 1 2 AILTILGNSAVLATAVKRSSLLKSPELLTVNLAVADIGMAISMYPLAIASAWNHAWLGGDASCIYYALMGFLFGVCSMMTLCAMAVIRFLVTNSSKSN 1 2 SNKISKNTVHILITFIWLYSLLWAILPLVGWGYYGPEPFGISCTIAWSKFHSSSNGFSFILSMFLLCTVLPALTIVACYLGIAWKVHKAYQEIQNINRIPHAAKLEKKLTL 0 0 MAVLISVGFLSAWTPYAAASFWSIFNSSDSLQPIVTLLPCLFAKSSTAYNPFIYYIFSKTFRHEIKQLQCCWGWRVHFFSADNSAENSVSMMWSGRDNIRLSPTAKVESQGAARH* >NEUR4_ornAna Ornithorhynchus anatinus (platypus) XM_001508128 0 MSLSHSLQVPWRNNLTFLNKEAQVSEQGETIIGIYLLAL 1 2 GWMSWFGNSMVIFILHRQRGILNPTDYLTFNLAVSDASVSVFGYSRGIIEIFNVFRDDGFLITSIWTCQ 0 0 VDGFLTLLFGLASINTLAMISVTRYIKGCHPHR 1 2 GHFINTANISVALILIWVSALFWSAGPVLGWGSYT 1 2 DRMYGTCEIDWAEANFSSICKSYIISIFFCCFFLPVSIMFFSYVSIIKMVKSSHTLAGADDPTDRQRRLDRDVTR 0 0 VSVVICTAFIVAWSPYAVISMWSAFGHSVPNLTSVLASLFAKSASFYNPIIYFGMNSKFRKDILVLLPCAKESKEPVKLKKFKNLRQKQGFTLQKPEKAHVLQVPDSGPMSLINTPPLGNRNSFDLACDNSDFECVRL* 0
Melanopsin (3+ marsupials)
Here Sarcophilus can be expected to have the main melanopsin but not the paralog MEL2 which terminates in sauropods
>MEL1_homSap Homo sapiens (human) Gq -GRID1 -WAPAL +LDB3 +BMPR1A 483 aa NM_033282 melanopsin OPN4 0 MNPPSGPRVPPSPTQEPSCMATPAPPSWWDSSQSSISSLGRLPSISPT 0 0 APGTWAAAWVPLPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2 1 SRSLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGET 1 2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1 2 SAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2 1 ALQTFGACKGNGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2 1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2 1 VAIAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTLTSHTSNLSWISIRRRQESLGSESEV 0 0 GWTHMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGHEAETPGK 0 0 TKGLIPSQDPRM* 0 >MEL1_proCap 0 MNPPWGPRVPSRPAQEPSCMSTPASAGRWDSSQATASSLAELPPSSPT 0 0 EARTQTADWVPFPTVDVPDYAHYTLGTVILLVGLTGVLGNLMVIYIFFR 2 1 SRGLRTPANMFIINLAISDFLMSLTQAPVFFASSLYKRWLFGEA 1 2 GCEFYAFCGALFGITSMITLTAIALDRYLVITRPLATIGVVSKRRTALVLLGTWLYALAWSLPPFFGW 1 2 SAYVPDGLLTSCSWDYKSFMPSARTYTMLLCCFVFFLPLLVIIYCYVFIFKAIRETGR 2 1 ALQTFGACEGASETPRQWQRLQSEWKMAKIALLAILLYVLSWAPYSTVALVGFAG 2 1 YAHVLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2 1 MAIAQHLPCLGVLLGVSDQHTRPYTSYRSTHHSTLSSQASDISWISGRRRQASLGSESEV 0 0 GWTDTEAAAAWEGAQQVSGRASCSQVLESMEANTPPRPQGWGPETPRK 0 0 VKGLPLLDPRA* 0 >MEL1_smiCra Sminthopsis crassicaudata (dunnart) DQ383281 0 MNPSPMLRHLSCPAQDSNCTKIMASISEWNNTEVDAYHLVDLPPITPT 0 0 AVVLPPYSQKVFPTADVPDYAHYTIGATILVVGFTGVLGNLLVIYTFCR 2 1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFASSLYERWIFGEK 2 1 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1 2 SAYVPEGLLTSCSWDYTTFTPSVRAYTILLFCFVFFIPLTVIIYCYIFIFRAIKDTNK 2 1 AVQNIGSSEHTPSLRHFQRMKNEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2 1 YSHVLTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2 1 MAIAQNFPCLRAVLGIRHPRTQSFSSYRFTHRSTTASQASDISWQSRGRRQLSLGSESEA 0 0 GWNNIETGLTLRSLEGSCGMDEETMDTRELSASTKAKGQSWETLAKTLEE 0 0 MDDLSLLEAGTLLSSLDLQI* 0 >MEL1_sarHar Sarcophilus harrisii (tasmanian_devil)96% identity smiCra last exon missing FKUJDAX01C1KMN needed 0 MNPSPMLRHLSCSAQDTNCTKIMASISEWNNTEVDAYHLVDLPPITPT 0 0 AVVLPPYSQNVFPTADVPDYAHYTIGATILVVGFTGVLGNLLVIYTFCR 2 1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFASSLYKRWIFGEK 2 1 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGMISKKKTGLILLGVWLYSLAWSLPPFFGW 1 2 sAYVPEGLLTSCSWDYTTFTPSVRAYTILLFCFVFFIPLIVIIYCYIFIFRAIKDTNK 2 1 AVQNIGSRASTPSPRHFQRMKNEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2 1 YSHVLTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2 1 MAIAQNFPCLRAVLGIRHPRTQSFSSYRFTHRSTTASQASDISWQSRGRRQLSLGSESEA 0 0 GWNNIEAGIEGLTLRSLEGYCGMDEETMETREPSASAKAKGQ 0 0 * 0 >MEL1_macEug Macropus eugenii frag 0 AVVLPPHSRNIFPTADVPDHAHYTVGAIILVVGFTGVLGNLLVIYTFCR 2 1 SRSLRTPANMFIINLAISDFFMSFTQAPVFFANSLYKRWIFGEK 2 2 GCEFYAFCGALFGITSMITLMVIALDRYFVITRPLASIGVVSKKKTGLILLGVWLYSLAWSLPPFFGW 1 2 AYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFKAIQDTNK 2 1 ALQNIRSSESTASPRHFQRMKSEWKMAKIALVVILLFVLSWAPYSTVALVAFAG 2 1 SHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2 >MEL1_monDom Monodelphis domestica (opossum) Gq -GRID1 -WAPAL +LDB3 +BMPR1A 0 MNPSPMLRGLSCPAQDTNCTKIMASMSEWNNTEEDAYHLVDLPSIAPT 0 0 AVVLPPSSQNIFPTVDVPDHAHYTIGAIILAVGITGMLGNFLVIYTFCR 2 1 SHSLRTPANMFIINLAISDFFMSFTQAPVFFASSMYKRWIFGEK 1 2 ACEFYAFCGALFGITSMITLMAIALDRYFVITRPLASIGVISKKKTGFILLGVWLYSLAWSLPPFFGW 1 2 SAYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFRAIQDTNK 2 1 AVHSIGSGESTASPRHCQRMKNEWKMAKIALVVILLYVLSWAPYSTVALVAFAG 2 1 YSHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2 1 MAIAQNFPCLRALLCVRHPRTRSFSSYRFTRRSTMTSQASDISWLPRGRRQLSLGSESEI 0 0 GWNNMEAGTTSLTSRNQQGSCRMDQETMETRELAAIAKAKGRSWETLEK 0 0 TLEEMDDSSLLEVSVDMEQ* 0 >MEL1_ornAna Ornithorhynchus anatinus (platypus) fragment 0 0 0 FPTADVPDHAHYTIGATILAVGFTGVLGNLLVIYTFCR 2 1 SRSLRTPANMFIINLSISDFFMSLTQAPVFFASSLHKRWIFGEK 1 2 GCQLYAFCGALFGITSMITLTVIALDRYFVITRPLASIGVISKKRALLILTGVWFYSLAWSLPPFFGW 1 2 sAYVPEGLLTSCSWDYMTFTPPVRAYTMLLFCFVFFIPLIMIIYCYFFIFRAIRGTNK 2 1 AVETIGSDDCRGSQRQCQRMKNEWKTAKIALMVILLYVISWCPYSVVALVAFAG 1 YSHLLTPYMNSVPAVIAKSSAIHNPIIYAITHPKYR 2 1 MAITKYIPCLGPLLRVSRQDSRSSSHYASSRRSTVTSQSLDGSWLPGRRRPLSSASDSES 0 0 0 0 * 0 >MEL1_anoCar Anolis carolinensis diverged frag 0 0 0 ERTMFNLPDPFPTVDVPTHAHYTIGAVILVVGITGTLGNLLVIYVFFR 2 1 IRGLRTPANMFVINLAVSDFL 1 2 GCELYAFCGALFGIASMITLTVIALDRYFVITRPLASIGAMSTKKALLILSGVWLYSLAWSLPPFFGW 1 2 sAYVPEGLLTSCSWDYITFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFIAIKNSNR 2 1 AVQRTNSDNSKEGQKLYQKLKNEWKMAKVALIVILLVISWSPYSVVALVAFAG 2 1 YSHLLTPYMNSVPAVIAKASVIHNPIIYAIVHPKYR 2 1 MAIAKFLPCLGSLLRVPRKDSSYPSTRRPTVTSQSSDINGVPRGHRRLSSVSDSES 0 0 DWTDTEADISSQNSRVASGSISYRIYEDTTETIKVKSKMRSHDSGIFER 0 0 0 0 TGEDLNAFGWRREESYSGPSTSSQIPSIIVTFSNVQRTDLPLESSSGALCSRNSSYSWEKDSNS* 0 >MEL1_galGal Gallus gallus (chicken) Gq short exon 1 -GRID1 -WAPAL +LDB3 +BMPR1A 529 aa 16856781 AY88294 melanopsin OPN4m 0 MDLPPRAPT 0 0 KMTVKDVRGAFPTVDVPDHAHYTIGTVILIVGITGTLGNFLVIYAFCR 2 1 SRTLQKPANIFIINLAVSDFLMSITQSPVFFTNSLHKRWIFGEK 1 2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVRVMSKKKALIILVGVWLYSLAWSLPPFFGW 1 2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFEAIKKANK 2 1 SVQTFGCKHGNRELQKQYHRMKNEWKLAKIALIVILLYVISWSPYSVVALVAFAG 2 1 YSHVLTPFMNSVPAVIAKASAIHNPIIYAITHPKYR 2 1 TAIATYVPCLGFLLRVSPKESRSFSSYPSSRRTTITSQSSETSGLQKGKRRLSSISDSES 0 0 GCTDTETDITSMISRPASSQVSYEMGEDTTQTSDLGGKPKVKSHDSGIFRK 0 0 TVVDADEIPMVEINDTEHSATSTCKTSEKCNVEEIQ 0 0 RSESLSGIGLREGESRHRTSASQIPSIIITYSNVQGVELHSGYSAGFLHPKNKSHKQNKSSNS* 0 >MEL2_galGal Gallus gallus (chicken) Gq 0.0.1.2.2.1.1.1.0.0 indel +GRID2 +SMARCAD1 -PGDS -SEC24B +COL25A1 544 aa 000 nm 17977531 NM_204625 full 0 MGTQPHSVTKSEIPDHVLYTVGTCVLVIGSIGIIGNLLVLYAFYS 2 1 NKKLRTPQNFFIMNLAVSDFLMSASQAPICFVNSLHREWILGDI 1 2 GCDLYAFCGALFGITSMMTLLAISVDRYLVITKPLRSIQWTSKKRTIQIIAAVWLYSLGW 1 2 SVAPLLGWSSYVPEGLMISCTWDYVTYSPANRSYTMILCCCVFFIPLIIILHCYLFMFLAIRSTGR 2 1 DVQKLGSCSRKSFLSQSMKNEWKLAKIAFVVIIVYVLSWSPYACVTLIAWAG 2 1 RGNTLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYR 2 1 KTIHNAVPCLRFLIRISKNDLLRGSINESSFRTSLSSHQSLAGRTKNTCVSSVSTGEA 0 0 NWSDVELDTVEPAHEKLQPRRSHSFSSSLRQKRDLLPDSYSCSEETEEK 0 0 VSLSSSYLEKVLGRSAFPSSPVALVTSSLRAASLPVGLNSSSASRGAGSDISQMKTEESHNNGGLDSIVSNTVPQIIIIPTSETNLFQEEPEEEETELFHFHDKKNNLLDLEGLSSSTEFLEAVEKFLS* 0
PRNP (3+ marsupials)
The Sarcophilus repeat region is of considerable interest -- the high GC content of this region makes it difficult to sequence and so provides a test of the 454 technology and Newbler assembler. This region consists in placentals a five octapeptide repeat, in marsupials and platypus a five nona- or decapeptide residue repeat that may resolve fine details of the marsupial phylogenetic tree, which in birds, lizards, turtles, frogs and fish is a hexapeptide repeat with trimeric internal substructure. Even though the single exon gene is clearly orthologous in all these species, the repeat regions within it are not directly comparable because they have expanded and contracted through replication slippage, plus experienced the odd repeat length change in marsupials and another in placentals.
The Sarcophilus prion gene has very high coverage that overcomes the occasional problem with frameshifts and allows the gene to be accurately tiled. However familiarity with the gene and reliable fiducial sequences are key to rapid assembly of the full length gene. No sequencing difficulties were observed in the high GC repeat region. The gene is very normal and has no indications whatsoever of abnormal numbers of repeats (4) or prion disease disposition.
Dasypus MVRSRVGCWLLLLFVATWSELGLC KK.RPKPGGGWNTGG SRYPGQ GSPGG NRYP PQGGG WGQ PHGGG WGQ PHGGG WGQ PHGGG WGQ PHGGG WGQ GGAHGQ Trichosurus MGKIQLGYWILVLFIVTWSDLGLC KKPKPRPGGGWNSGGS NRYPGQPGSPGG NRYPGWGH PQGGGTNWGQ PHPGGSNWGQ PHPGGSSWGQ PH GGSNWGQ GG YN Sarcophilus MGKIRLGYWILALFIVTWSDLGLC KKPKPRPGGGWNSGGS NRYPGQPGSAGG NRYPGWGH PQGGGTNWGQ PHPGGSSWGQ PHAGGSNWGQ PH.GGSNWGQ SGSSYNQ Monodelphis MGKIHLGYWFLALFIMTWSDLTLC KKPKPRPGGGWNSGG NRYPGQ SG GWGH PQGGGTNWGQ PHAGGSNWGQ PRPGGSNWGQ PHPGGSNWGQ PHPGGSNWGQ AGSSYNQ Macropus MAKIQLGYWILALFIVTWSELGLC KKPKTRPGGGWNSGGS NRYPGQPGSPGG NRYPGWGH PQGGGTNWGQ PHPGGSSWGQ PHAGGSNWGQ PH.GGSNWGQ GGGSYG Ornithorhynchus ------------------------ -------GGGWNSG NRYPGQPANPG GWGH PQGGGASWGH PQGGGASWGH PQGGGSNWGH PQGGGASWGH PQ GGGYS Dasypus WNKPSKPKTNM KHVAGAAAAGAVVG LGGYLVGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRSVEQYSSEKNFVHD CV MERVVEQMCITQYQ Trichosurus KWKPDKPKTNL KHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFGNEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN Sarcophilus KWKPDKPKTNM KHMAGAAAAGAVLGSLGGYVLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN Monodelphis KWKPDKPKTNM KHVAGAAAAGAVVGGLGGYMLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYNNQNNFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN Macropus KWKPDKPKTNL KHVAGAAAAGAVVGGLGGYMLGSAMSRPVMHFGNEYEDRYYRENQYRYPNQVMYRPIDQYGSQNSFVHD CVNITVKQHTTTTTTKGENFTETDIKIMERVVEQMCITQYQN Ornithorhynchus KYKPDKPKTGM KHVAGAAAAGAVVGGLGGYMIGSAMSRPPMHFGNEFEDRYYRENQNRYPNQVYYRPVDHFCSQDGFVRD CVNITVTQHTVTTT.EGKNLNETDVKIMTRVLEQMC
The signal region of Sarcophilus PRNP is expected to show the same length as the other 3 known marsupial sequences, which is confirmed by the sequence. Placentals exhibit a one residue deletion relative to this ancestral length.
MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Homo sapiens MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Pan troglodytes MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Gorilla gorilla MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Pongo pygmaeus MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Nomascus leucogenys MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Hylobates lar MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Symphalangus syndactylus MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca arctoides MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca fascicularis MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca fuscata MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca mulatta MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Macaca nemestrina MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Papio hamadryas MA--NLGCWMLFLFVATWSDLGLCKK--RPKPG Callithrix jacchus MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Cebus apella MA--NLGCWMLVVFVATWSDLGLCKK--RPKPG Cercopithecus aethiops MA--NLGCWMLVVFVATWSDLGLCKK--RPKPG Cercopithecus dianae MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Colobus guereza MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Presbytis francoisi MA--NLGCWMLVLFVATWSDLGLCKK--RPKPG Saimiri sciureus MA--KLGYWLLVLFVATWSDVGLCKK--RPKPG Tarsius syrichta MA--NLGCWMLVVFVATWSDVGLCKK--RPKPG Microcebus murinus MA--RLGCWMLVLFVATWSDIGLCKK--RPKPG Otolemur garnettii ME--NLGCWMLILFVATWSDIGLCKK--RPKPG Cynocephalus variegatus MA--QLGCWLMVLFVATWSDVGLCKK--RPKPG Tupaia belangeri MA--NLGYWLLALFVTMWTDVGLCKK--RPKPG Mus musculus MA--NLGYWLLALFVTTCTDVGLCKK--RPKPG Rattus norvegicus MA--NAGCWLLVLFVATWSDTGLCKK--RPKPG Cavia porcellus MA--NLGCWLLVLFVATWSDLGLCKK--RTKPG Dipodomys ordii MV--NPGCWLLVLFVATLSDVGLCKK--RPKPG Spermophilus tridecemlineatus MA--HLGYWMLLLFVATWSDVGLCKK--RPKPG Oryctolagus cuniculus MA--HLSYWLLVLFVAAWSDVGLCKK--RPKPG Ochotona princeps MVKSHIGSWILVLFVAMWSDVGLCKK--RPKPG Bos taurus MVKSHIGGWILVLFVAAWSDIGLCKK--RPKPG Sus scrofa MVKSHMGSWILVLFVVTWSDMGLCKK--RPKPG Vicugna vicugna MVKSHVGGWILVLFVATWSDVGLCKK--RPKPG Equus caballus MVRSHVGGWILVLFVATWSDVGLCKK--RPKPG Diceros bicornis MVKSLVGGWILLLFVATWSDVGLCKK--RPKPG Myotis lucifugus MVKNYIGGWILVLFVATWSDVGLCKK--RPKPG Pteropus vampyrus MVKSHIANWILVLFVATWSDMGFCKK--RPKPG Tursiops truncatus MVKSHIGGWILLLFVATWSDVGLCKK--RPKPG Canis lupus familiaris MVKSHIGSWILVLFVAMWSDVGLCKK--RPKPG Felis catus MVKSHIGSWLLVLFVATWSDIGFCKK--RPKPG Mustela putorius MVKSHIGSWLLVLFVATWSDIGFCKK--RPKPG Mustela vison MVKSHIGSWILVLFVAMWSDVGLCKK--RPKPG Ailuropoda melanoleuca MVKNHVGCWLLVLFVATWSEVGLCKK--RPKPG Erinaceus europaeus MVTGHLGCWLLVLFMATWSDVGLCKK--RPKPG Sorex araneus MVKSHLGCWIMVLFVATWSEVGLCKK--RPKPG Cyclopes didactylus MVRSRVGCWLLLLFVATWSELGLCKK--RPKPG Dasypus novemcinctus MVKGTVSCWLLVLVVAACSDMGLCKK--RPKPG Echinops telfairi MVKSSLGCWILVLFVATWSDMGLCKK--RPKPG Loxodonta africana MVKSSLGCWMLVLFVATWSDVGLCKK--RPKPG Procavia capensis MAKIQLGYWILALFIVTWSELGLCKKP-KTRPG Macropus eugenii MGKIHLGYWFLALFIMTWSDLTLCKKP-KPRPG Monodelphis domestica MGKIRLGYWILALFIVTWSDLGLCKKP-KPRPG Sacophilus harrisii MGKIQLGYWILVLFIVTWSDLGLCKKP-KPRPG Trichosurus vulpecular MARLLTTCCLLALLLAACTDVALSKKG-KGKPS Gallus gallus MAKLPGTSCLLLLLLLLGADLASCKKG-KGKPG Taeniopygia guttata MARLLTTCCLLALLLAACTDVALSKKG-KGKPG Meleagris gallopavo MGKHQMTCWLAIFLLLIQANVSLAKK--KPKPS Anolis carolinensis MRRFLVTCWIAVFLILLQTDVSLSKKG-KNKPG Gekko gekkko MGRYRLTCWIVVLLVVMWSDVSFSKKG-KGKGG Trachemys scripta (turtle) MGRHLISCWIIVLFVAMWSDVSLAKKG-KGKTG Pelodiscus sinensis (turtle) MPQSLWTCLVLISLICTLTVSSKKSGGGKSKTG Xenopus laevis MLRSLWTSLVLISLVCALTVSSKKSGSGKSKTG Xenopus topicalis
>PRNP_sacHar Sarcophilus harrisii (tasmanian_devil) single exon gene YVLG like Dasypus MGKIRLGYWILALFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGSAGGNRYPGWGHPQGGGTNWGQPHPGGSSWGQPHAGGSNWGQPHGGSNWGQ SGSSYNQKWKPDKPKTNMKHMAGAAAAGAVLGGVGGYVLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTTTT KGENFTETDIKIMERVVEQMCITQYQNEYRAAQYSYNMAFFSAPPVTLLLLGFLIFLIVS* >PRNP_mdo Monodelphis domestica opossum, from frameshifted genomic MGKIHLGYWFLALFIMTWSDLTLCKKPKPRPGGGWNSGGNRYPGQSGGWGHPQGGGTNWGQPHAGGSNWGQPRPGGSNWGQPHPGGSNWGQPHPGGSNWG QAGSSYNQKWKPDKPKTNMKHVAGAAAAGAVVGGLGGYMLGSAMSRPIMHFGNDYEDRYYRENQYRYPNQVMYRPIDQYNNQNNFVHDCVNITVKQHTTT TTTKGENFTETDIKIMERVVEQMCITQYQNEYRSAYSVAFFSAPPVTLLLLSFLIFLIVS* >PRNP_tvu Trichosurus vulpecular brushtail opossum MGKIQLGYWILVLFIVTWSDLGLCKKPKPRPGGGWNSGGSNRYPGQPGSPGGNRYPGWGHPQGGGTNWGQPHPGGSNWGQPHPGGSSWGQPHGGSNWGQGGY NKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVIHFGNEYEDRYYRENQYRYPNQVMYRPIDQYSSQNNFVHDCVNITVKQHTTTTTTKGENFTETDIKIMERVVEQM CITQYQAEYEAAAQRAYNMAFFSAPPVTLLFLSFLIFLIVS* >PRNP_meu Macropus eugenii (tammar wallaby) MAKIQLGYWILALFIVTWSELGLCKKPKTRPGGGWNSGGSNRYPGQPGSPGGNRYPGWGHPQGGGTNWGQPHPGGSSWGQPHAGGSNWGQPHGGSNWGQ GGGSYGKWKPDKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPVMHFGNEYEDRYYRENQYRYPNQVMYRPIDQYGSQNSFVHDCVNITVKQHTTTTTT KGENFTETDIKIMERVVEQMCITQYQNEYQAAQRYYNMAFFSAPPVTLLLLSFLIFLIVS* >PRNP_oan Ornithorhynchus anatinus platypus fragment PHWGKSPVHHWIIDICVVHLERRCRGHLHPNPCPGGRCVQQQPNRYPGQPATPGGWGHPQGGGASWGHPQGGGSNWGHPQGGGASWGHPQGGGYSKYKPDKPKTG MKHVAGAAAAGAVVGGLGGYMIGSAMSRPPMHFGNEFEDRYYRENQNRYSNQVYYRPVDQYGSQDGFVRDCVNITVTQHTVTTTEGKNLNETDVKIMTRVLEQMCVNLY
PRND (2+ marsupials)
Sarcophilus sequence for this intronless gene is a welcome addition to a limited existing set of early-diverging mammalian orthologs. With more data, the relative rates of divergence of PRND from its parental paralog PRNP could be compared in marsupial and placentals. It appears from the mere 75% identity between tasmanian devil and wallaby that doppels are diverging quite rapidly both from PRNP and from each other in the marsupial lineage, indicating some selectional pressure but not a hugely important function (that is, many residue positions have an increased reduced alphabet).
>PRND_hsa Homo sapiens (human) full MRKHLSWWWLATVCMLLFSHLSAVQTRGIKHRIKWNRKALPSTAQITEAQVAENRPGAFIKQGRKLDIDFGAEGNRYYEANYWQFPDGIHYNGCSEANVTKEAFVTGCINATQAANQGEFQKPDNKLHQQVLWRLVQELCSLKHCEFWLERGAGLRVTMHQPVLLCLLALIWLTVK* >PRND_dno Dasypus novemcinctus MRKHLGGWRLAIVCVLLSGHLSMVKARGIKHRIKWNRKAAPGAAQVTEARVAEQRPGAFVRQGRRLDIDFGAEGNRYYEANYWQLPDGILYDGCAEANVTKEALVAGCVNATQLANQAELAHEGQDTLHRRVLGRLIRELCALKRCKFWPDRAAGPRLVRGAPVFGGLLLLIWLLVR* >PRND_laf Loxodonta africana African elephant Afrotheria 176 aa revised/corrected MRKHLGAWWLAIAFVLLLSHLSMVTARGIKHRIKWNRKALPNTGHVTAAQVTETRPGAFIRHGRKLDIDFGAEGNRYYEANYWQFPDGIHYDGCSEANVTKEMFVTSCINTTQAANQEEFSRKQDNKVYQRILWRLIRELCSVKHCDFWLDRGGGLRVSLDQPVMLCLLVFIWFMVK* >PRND_sacHar Sarcophilus harrisii (tasmanian_devil) single exon gene 77% macEug MRTPLETWWIAIFFTLLFSDLSLVKAKGIRQRNKSNRKSLQTNRANPTREQPSKILQGTFIRKGRKLSINFGEEGNSYYEAHYKLFPDEIHYVGCAESSVTKDVFISNCVNVTHTANKLEPPEERNSSAIYSRVLEQLIKELCALKYCEFGMQIGAGFRLSLDQSMMVYLMILAFFIVK* >PRND_mdo Monodelphis domestica doppel genomic revised +rassf2 -prnd -prnp MRRHLGICWIAIFFALLFSDLSLVKAKTTRQRNKSNRKGLQTNRTNPTTVQPSEKLQGTFIRNGRKLVIDFGEEGNSYYATHYSLFPDEIHYAGCAESNVTKEVFISNCVNATRVINKLEPLEEQNISDIYSRILEQLIKELCALNYCEFRTGKGTGLRLSLDQYVMVYLVILTCLIVK* >PRND_meu Macropus eugenii wallaby MRRHLGTWWTAIFFALLFSDLSLVKAKGTRQRNKSNRKSLQTNRVNPTTAQPSEILQGAFIRQGRKLSIDFGEEGNSYYETHYQLFPDEIHYVGCTESNVTKDIFISNCMNATHAVNNLETLEEKNASDIHSRVLEQLIKELCALKYCELETETGAGLKLSLDQSVMVYLVILTCLIVK* >PRND_oan Ornithorhynchus anatinus platypus 42% to opposum 187 aa 4 cys in register MMTVRRRRRSGGARWLLVFLVLLSGDLSSLQARGPRPRNKAGRKPPPSNAGPDSPAPRPPAGARGTFIRRGGRLSVDFGPEGNGYYQANYPLLPDAIVYPDCPTANGTREAFFGDCVNATHEANRGELTAGGNASDVHVRVLLRLVEELCALRDCGPALPTGPAPRPGPPGPPAALALLTLVLLGAQ* >PRND_aca Anolis carolinensis weak but real! scaffold_1221:78,884-117,121 syntenic, oriented like PRNP but no larger MMQRPLVVAILLTALWSEVCLCRRVSGSANRRNKKTSTTTSAPKLQSSTTATTFQGNLCRGGQMIDNMDLEPNDKVYYKANLKIFPDGLYYPNCSLLLQPNTTKEELVGECVNFTIASNKLNLSKGKDLSNTKERVMWVLIHHLCANESCGQPCPLLQNSGNLHYIGQVLTVFVGLIGCSFLSAK*