PslProtCnv

The Custom Track CGI (hgCustom) is not able to display protein-based psl tracks. This script will convert protein-based psl output into nucleic-acid-based psl output. The resulting output can be used in hgCustom.

#!/usr/bin/awk -f
#
# pslProtCnv protPsl > naLikePsl
#   Convert a protein PSL to have block sizes in units of nucleic acids
#   so it will work as custom track.  Note that this will ocassionally
#   create overlapping blocks, which will still display in the browser.
#
BEGIN {
    FS = OFS = "\t";
}
!/^[0-9]+/ {
    print $0; # pass through track lines, etc
    next;
}
{
    strand=$9;
    qSize=$11;
    qStart=$12;
    qEnd=$13;
    tSize=$15;
    blkCnt=$18;
    blkSizesStr=$19;
    qStartsStr=$20;
    tStartsStr=$21;

    # make strand untranslated, check for rev cmpl
    newStrand = (strand == "+-") ? "-" : "+";
    rc = (strand == "+-");

    split(blkSizesStr, blkSizes, ",");
    split(qStartsStr, qStarts, ",");
    split(tStartsStr, tStarts, ",");

    newBlkSizesStr = newQStartsStr = newTStartsStr = "";

    for (i = 1; i <= blkCnt; i++) {
        # get adjusted block size and starts
        bs = 3*blkSizes[i];
        if (rc) {
            qs = 3*(qSize-(qStarts[i]+blkSizes[i]));
            ts = (tSize-(tStarts[i]+bs));
        } else {
            qs = 3*qStarts[i];
            ts = tStarts[i];
        }
        # add to comma-separated columns
        if (rc) {
            newBlkSizesStr = bs "," newBlkSizesStr;
            newQStartsStr = qs "," newQStartsStr;
            newTStartsStr =   ts "," newTStartsStr;
        } else {
            newBlkSizesStr = newBlkSizesStr bs ",";
            newQStartsStr = newQStartsStr qs ",";
            newTStartsStr = newTStartsStr ts ",";
        }
    }
    $9 = newStrand;
    $11 = 3*qSize
    $12 = 3*qStart;
    $13 = 3*qEnd;
    $19 = newBlkSizesStr;
    $20 = newQStartsStr;
    $21 = newTStartsStr;
    print $0;
}