Microarray track

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This document explains the underlying data format of the microarray annotation tracks displayed on the UCSC Genome Browser. Note that the Gene Sorter has a somewhat different strategy for displaying microarray data that is not discussed here.

Database Representation

Microarray track data are stored in "BED 15" format. A typical line in a gapped BED file has 12 fields. Microarray, or "expRatio", BEDs, have an additional three fields:expCount, expIds, and expScores.

Here is an example of BED 15 format: 

#chrom	chromStart	chromEnd	name	score	strand	thickStart	thickEnd	reserved	blockCount	blockSizes	chromStarts	expCount	expIds	expScores
chr1	159639972	159640031	2440848	500	-	159639972	159640031	0	1	59,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	0.593000,1.196000,-0.190000,-1.088000,0.093000,-0.731000,0.130000,-0.008000,-1.087000,0.609000,-1.061000,-1.092000,0.807000,0.499000,-0.322000,-0.985000,0.309000,0.000000,0.812000,-0.457000,-0.560000,0.096000,0.186000,-1.092000,0.045000,0.573000,1.170000,1.336000,1.251000,1.919000,-0.056000,-0.189000,0.028000,
chr1	159640161	159640190	2440849	500	-	159640161	159640190	0	1	29,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	-0.906000,-1.247000,0.111000,-0.515000,-0.057000,-0.892000,0.167000,1.278000,0.051000,-0.596000,-0.251000,-0.826000,0.487000,0.714000,0.674000,1.046000,0.694000,0.236000,-0.718000,-1.196000,-1.274000,-1.278000,-1.055000,0.838000,-0.494000,1.137000,0.000000,0.690000,0.166000,-0.232000,0.174000,-1.253000,1.363000,
chr1	159640215	159640242	2440850	500	-	159640215	159640242	0	1	27,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	-0.465000,0.127000,1.215000,-0.073000,-0.465000,-0.141000,0.507000,-0.462000,-0.464000,0.570000,1.356000,0.559000,-0.459000,-0.464000,-0.458000,0.000000,0.322000,-0.454000,0.887000,-0.464000,1.196000,-0.463000,0.376000,-0.461000,0.547000,0.032000,-0.464000,0.066000,0.762000,-0.465000,-0.456000,0.919000,-0.464000,
chr1	159640256	159640309	2440851	500	-	159640256	159640309	0	1	53,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	1.779000,1.039000,-0.068000,-0.066000,-0.050000,0.665000,0.861000,-0.067000,0.000000,0.090000,-0.067000,1.240000,-0.018000,-0.068000,0.122000,0.478000,-0.068000,-0.068000,0.630000,-0.068000,0.092000,0.620000,-0.066000,-0.068000,1.601000,0.537000,1.103000,0.720000,1.959000,1.703000,-0.061000,-0.067000,1.097000,
chr1	159641139	159641250	2440852	300	-	159641139	159641250	0	1	111,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	0.000000,1.569000,-0.090000,0.330000,-0.091000,-0.089000,0.634000,1.319000,-0.090000,-0.092000,-0.048000,-0.091000,-0.091000,0.815000,1.455000,1.127000,1.549000,0.337000,0.432000,-0.092000,-0.092000,-0.090000,0.397000,1.082000,-0.092000,-0.091000,1.780000,2.025000,1.988000,2.575000,0.224000,-0.092000,-0.092000,
chr1	159642074	159642102	2440853	500	-	159642074	159642102	0	1	28,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	2.204000,-1.053000,-0.756000,1.135000,0.393000,0.366000,-0.730000,-0.113000,-1.179000,1.079000,-1.194000,-1.195000,-0.675000,1.124000,0.382000,-1.196000,0.313000,-0.380000,0.244000,-0.258000,2.070000,1.312000,-1.195000,1.901000,-1.191000,0.438000,-1.195000,1.096000,1.635000,0.232000,-0.440000,0.528000,0.000000,
chr1	159642586	159642611	2363661	600	+	159642586	159642611	0	1	25,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	0.486000,0.065000,0.897000,-0.566000,-1.169000,-1.547000,-0.266000,0.370000,-0.864000,-0.802000,1.214000,-0.240000,0.269000,-0.067000,0.375000,0.258000,0.366000,-0.275000,0.965000,0.785000,0.571000,0.511000,0.759000,0.658000,-0.196000,-0.055000,-0.017000,-0.411000,-0.450000,-1.028000,0.104000,-0.228000,-0.000000,
chr1	159642702	159642762	2363662	600	+	159642702	159642762	0	1	60,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	-0.772000,-0.532000,0.061000,-1.913000,-2.283000,-1.696000,-0.062000,0.200000,-0.609000,-2.054000,-1.184000,-2.465000,0.746000,0.673000,0.316000,0.041000,1.147000,0.590000,0.963000,0.919000,0.914000,0.000000,0.224000,-0.077000,0.395000,1.124000,0.690000,-0.369000,-1.427000,-0.970000,0.297000,0.335000,1.471000,
chr1	159642857	159642883	2363663	200	+	159642857	159642883	0	1	26,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	0.434000,-1.679000,-1.102000,0.529000,0.435000,0.614000,0.000000,0.175000,-1.517000,-1.148000,-0.180000,-1.379000,-0.798000,-0.163000,-0.219000,-1.936000,0.183000,0.627000,-0.933000,0.446000,1.150000,-2.162000,-0.018000,0.191000,1.351000,1.378000,-0.941000,0.743000,-1.632000,-0.415000,0.800000,-0.165000,1.257000,
chr1	159642959	159642988	2363664	600	+	159642959	159642988	0	1	29,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	0.000000,0.018000,-0.061000,-1.540000,-1.477000,-1.977000,-0.058000,-0.430000,-0.293000,-1.256000,-1.060000,-1.701000,0.715000,0.978000,0.980000,1.190000,1.248000,0.974000,0.907000,1.145000,0.782000,-0.152000,0.218000,-0.109000,1.550000,1.126000,1.537000,0.023000,-0.140000,-0.728000,0.520000,0.605000,0.597000,
chr1	159643028	159643054	2363665	600	+	159643028	159643054	0	1	26,	0,	33	0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,	0.291000,-0.093000,0.000000,-1.483000,-1.041000,-1.644000,0.219000,-0.189000,-0.466000,-0.942000,-0.832000,-1.026000,0.565000,0.868000,0.813000,0.786000,0.793000,0.574000,0.773000,0.764000,0.568000,-0.291000,-0.055000,-0.103000,0.916000,0.970000,0.993000,-0.106000,-0.230000,-0.402000,0.310000,0.433000,0.563000,

The expCount field indicates how many microarrays the track is using. The expIds field is used to link microarray labels to the microarray measurements in the expScores field. The microarray labeling information is not found in the BED 15 at all; instead, it's part of the microarray configuration. Also, the expCount and expIds fields are constant across all rows. This may seem like a waste of space, but in theory the BED 15 spec allows more flexibility than it is actually given by its implementation in the browser.

trackDb Settings

To display correctly in the Genome Browser, microarray tracks require the setting of several specific trackDb attributes. Here is a sample trackDb entry for a microarray track: 

track affyHumanExon
shortLabel Affy All Exon
longLabel Affymetrix All Exon Chips
group regulation
priority 79.1
visibility hide
type expRatio
expScale 3.0
expStep 0.5
groupings affyHumanExonGroups

Of particular importance to microarray tracks are the type, expScale, expStep, and groupings parameters:

  • type -- setting this parameter to "expRatio" indicates to the browser that the track's data set is a microarray.
  • expScale -- an absolute value that reflects the dynamic range of the data and influences the coloring of the track. For example, an expScale setting of 3.0 indicates that most of the data lie between -3.0 and 3.0; the brightest green will be used for values less than or equal to -3.0 and the brightest red for values greater than or equal to 3.0.
  • expStep -- controls the color key step values on the details page.
  • groupings -- indicates the specific set of configurations to load from the microarrayGroups.ra file(s).

microarrayGroups.ra

The microarrayGroups.ra files are located in kent/src/hg/makeDb/hgCgiData in a directory/file structure similar to trackDb and hgNearData. During compilation and installation, these are copied to the apache cgi-bin where various CGIs read them directly. Like other configuration .ra files, the microarrayGroups.ra files are meant to be relatively small, compared to databases, and flexibly structured. 

name affyHumanExonGroups
type groupings
all affyHumanExonAll
combine affyHumanExonGroupByTissueMean,affyHumanExonGroupByTissueMedian,
subset affyHumanExonSubsetByTissue,affyHumanExonSubsetByReplicate,
combine.default affyHumanExonGroupByTissueMedian 

name affyHumanExonAll
type all
description All Arrays
expIds 0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,
groupSizes 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,
names breast_A,breast_B,breast_C,cerebellum_A,cerebellum_B,cerebellum_C,heart_A,heart_B,heart_C,kidney_A,kidney_B,kidney_C,liver_A,liver_B,liver_C,muscle_A,muscle_B,muscle_C,pancreas_A,pancreas_B,pancreas_C,prostate_A,prostate_B,prostate_C,spleen_A,spleen_B,spleen_C,testes_A,testes_B,testes_C,thyroid_A,thyroid_B,thyroid_C, 

name affyHumanExonGroupByTissueMean
type combine mean
description Arrays Grouped By Tissue Mean
expIds 0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,
groupSizes 3,3,3,3,3,3,3,3,3,3,3,
names breast,cerebellum,heart,kidney,liver,muscle,pancreas,prostate,spleen,testes,thyroid,

name affyHumanExonGroupByTissueMedian
type combine median
description Arrays Grouped By Tissue Median
expIds 0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,
groupSizes 3,3,3,3,3,3,3,3,3,3,3,
names breast,cerebellum,heart,kidney,liver,muscle,pancreas,prostate,spleen,testes,thyroid,

Each paragraph of the microarrayGroups.ra file has the same basic fields name and type. In addition, with the exception of paragraphs with "type groupings", the paragraphs also have the description, expIds, groupSizes, and names fields.

The "type groupings" paragraph is linked to the trackDb setting "groupings" through the "name" setting. This paragraph will also define which other other paragraphs (perhaps subparagraphs) are connected with it. The all setting is required and points to the paragraph defining entirity of the arrays, ungrouped. The combine setting lists the paragraphs defining the other grouping methods for the set of arrays, and the combine.default mentions which of those is the default grouping method.

The "type all" and "type combine" paragraphs have similar formats. The description setting is required and it appears on the label of the track on the browser. The expIds correspond to the same expIds as the BED, but unlike the BED, these are meant to be in any order. The groupSizes and names settings both have the same number of words in the comma-separated lists and the order is important. In the affyHumanExonGroupByTissueMedian paragraph, the first of the names is "breast". The first of the groupSizes is "3", so the "breast" expIds are 0,1,2. Similarly, for cerebellum, the second groupSizes is 3, so those expIds are 3,4,5

Finally, if the paragraph type is "combine", then it requires and additional setting indicating how the arrays are combined. The two valid values are "median" and "mean".

Microarray Custom Tracks

The microarray custom track format is similar to the normal BED custom track format with the addition of three required track line parameters in the header (expNames, expScale, and expStep) that mimic similar settings in the trackDb and microarrayGroups.ra files.

Here is an example of a custom track file that uses the BED 15 data above, but displays only 5 of the 33 arrays: 

track type="array" expScale=3.0 expStep=0.5 expNames="breast_A,breast_B,breast_C,cerebellum_A,cerebellum_B," name="Microarray" description="Microarray custom track"
chr1	159639972	159640031	2440848	500	-	159639972	159640031	0	1	59,	0,	5	0,1,2,3,4,	0.593000,1.196000,-0.190000,-1.088000,0.093000,
chr1	159640161	159640190	2440849	500	-	159640161	159640190	0	1	29,	0,	5	0,1,2,3,4,	-0.906000,-1.247000,0.111000,-0.515000,-0.057000,

In this example:

  • expNames corresponds to the "names" setting in trackDb
  • expScale corresponds to the "expScale" setting in microarrayGroups.ra
  • expStep corresponds to the "expStep" setting in microarrayGroups.ra

These three parameters must be set if the type parameter is set to "array".

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